CN103194388A - Double-layer cell co-culture device with adjustable gap - Google Patents
Double-layer cell co-culture device with adjustable gap Download PDFInfo
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- CN103194388A CN103194388A CN2013100935870A CN201310093587A CN103194388A CN 103194388 A CN103194388 A CN 103194388A CN 2013100935870 A CN2013100935870 A CN 2013100935870A CN 201310093587 A CN201310093587 A CN 201310093587A CN 103194388 A CN103194388 A CN 103194388A
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Abstract
The invention discloses a double-layer cell co-culture device with an adjustable gap. The double-layer cell co-culture device comprises a cell culture box, a cell culture groove and a driving part, wherein the cell culture box is used for culturing a first cell; the cell culture groove is used for culturing a second cell, and is arranged in the cell culture box in a removable manner; and the driving part drives the cell culture groove to move to the bottom of the cell culture box in the cell culture box, and can adjust the gap between the bottom of the cell culture box and the bottom of the cell culture groove to the predetermined distance. According to the double-layer cell co-culture device provided by the invention, the driving part drives the cell culture groove to move to the bottom of the cell culture box, and thus, the distance between the double layers of cells is adjusted in an on-line manner, the gap can adjust the growth and the function of the cells, so that the adjustable growing environment is provided for cell co-culture, and the feasible device is provided for exploring a cell co-culture technology.
Description
Technical field
The present invention relates to a kind of for cytobiology, biomedicine, and the cell co-culture device of biomechanics Research.
Background technology
Altogether culture technique be with two kinds and two or more cell co-cultivation in the isometric growth environment, need different cellular layers to cultivate at interval, so that different cell interaction is grown jointly.For example, when the human hepatocyte is studied, need various kinds of cell to cultivate altogether.
At present, what mainly adopt is the intercellular cultivation altogether of fixed spacing, has the single problem of experimental result in the co-culture of cells experiment.
Summary of the invention
Problem at the prior art existence, the object of the present invention is to provide a kind of double-deck cell co-culture device of adjustable gaps, distance between two confluent monolayer cells can onlinely be regulated, realize the regulating effect of gap cell growth and function thereof, for co-culture of cells provides a kind of adjustable growing environment.
The double-deck cell co-culture device of a kind of adjustable gaps of the present invention comprises:
Cell culture case is used for cultivating first cell;
The cell cultures groove is used for cultivating second cell, and described cell cultures groove is removably set in the described cell culture case;
Driving part drives described cell cultures groove bottom to described cell culture case in described cell culture case and moves, with the gap of the bottom of the bottom of adjusting described cell culture case and described cell cultures groove to predetermined distance.
Preferably, be formed with the spring plectrum of the described cell cultures groove of resiliency supported on the described cell culture case.
Preferably, described driving part is to be arranged on the sliding sash that can move along described cell culture case length direction on the described cell culture case and to be arranged on the push rod that described sliding sash moves that promotes on the described cell culture case, be formed with the inclined-plane at described cell cultures groove, described sliding sash be formed with described inclined-plane suitable be used for to promote described cell cultures groove to the mobile skewback in described cell culture case bottom.
The present invention drives the cell cultures groove by driving part and moves to the bottom of cell culture case, like this, distance between two confluent monolayer cells can onlinely be regulated, realize the regulating effect of gap cell growth and function thereof, for co-culture of cells provides a kind of adjustable growing environment, provide a kind of feasible device for exploring the co-culture of cells technology, but the cell cultures of this device simulated animal internal milieu, and realize the growing environment unanimity of vivo and vitro as much as possible, thereby make iuntercellular exchange of information mutually, the growth increment of mutually promoting.Two kinds and two or more cell co-cultivation in the isometric growth environment, are impelled cell proliferation, kept cell biological function, and utilize different products of cellular metabolism regulating cell functions.
Description of drawings
Fig. 1 is structure partition synoptic diagram of the present invention;
Fig. 2 is installed in structural representation in the cell culture case for cell cultures groove among Fig. 1;
Fig. 3 is use view of the present invention.
Embodiment
As shown in Figure 1, the present invention includes: cell culture case 1, cell cultures groove 2 and driving part 3, cell culture case 1 is used for cultivating first cell.Cell cultures groove 2 is used for cultivating second cell, and cell cultures groove 2 is removably set in the cell culture case 1.Driving part 3 is used for driving cell cultures groove 2 and moves to the bottom of cell culture case 1 at cell culture case 1, with the gap of the bottom of adjusting cell culture case 1 and the bottom of cell cultures groove 2 to the distance of being scheduled to.
As shown in Figure 1, in embodiments of the present invention, be provided with sidewall 11 in the cell culture case 1, be formed with the spring plectrum 12 of resiliency supported cell cultures groove 2 on the sidewall 11.One end of spring plectrum 12 is formed on the sidewall 11, and the other end is formed be used to the block tongue 13 of holding cell cultures groove 2.Be formed with for the draw-in groove 22 against block tongue 13 at cell cultures groove 2.
As shown in Figure 1, driving part 3 is for being arranged on the sliding sash 31 that can move along cell culture case 1 length direction on the cell culture case 1 and being arranged on the push rod 33 that the promoted sliding sash 31 on the cell culture case 1 moves, and push rod 33 is installed on the cell culture case 1 by axle sleeve 34.Be formed with inclined-plane 21 at cell cultures groove 2, be formed with suitable being used in inclined-plane 21 at sliding sash 31 and promote the skewback 32 that cell cultures groove 2 moves to cell culture case 1 bottom.
Like this, as shown in Figure 1, 2, move promotion sliding sash 31 by push rod 33 and move, the skewback 32 on the sliding sash 31 is just exerted pressure to the inclined-plane, moves to the bottom of cell culture case 1 thereby drive cell cultures groove 2.Spring plectrum 12 can play the effect in gap between the bottom of the bottom of being convenient to support and adjust cell cultures groove 2 and cell culture case 1.
During use, at the bottom surface of the cell cultures groove 2 bonding liquid film of silica gel, the bottom is vesicular, and bonding liquid film is smooth.Cell is grown on the film.Another kind of cell is cultivated in cell culture case 1 bottom surface.Cell cultures groove 2 bottom surfaces and cell culture case 1 bottom surface adjustable distance can be from 0～4mm.
As shown in Figure 3, the present invention is installed to cell culture case 1 on the fixed frame 4 in use, and fixed frame 4 is provided with stepper-motor 5, also is provided with the pushing block 6 and the screw mechanism 7 that movably are used for push rod 33 on fixed frame 4.Stepper-motor 5 moves pushing block 6 by driving screw mechanism 7, and pushing block 6 promotion push rods 33 move like this, thereby has realized the adjustment in the gap between the bottom of the bottom of cell cultures groove 2 and cell culture case 1.In addition, as shown in Figure 3, can many group cell culture cases 1 be installed at fixed frame 4, carry out the adjustment in gap by pushing block 6 simultaneously.
The double-deck cell co-culture device of adjustable gaps of the present invention is the device of the cell cultures of simulated animal internal milieu.Cell cultures realizes the growing environment unanimity of vivo and vitro as much as possible, thereby makes iuntercellular exchange of information mutually, the growth increment of mutually promoting.Altogether culture technique be with two kinds and two or more cell co-cultivation in the isometric growth environment, impel cell proliferation, keep cell biological function, and utilize different products of cellular metabolism regulating cell functions.
Spacing is the key factor of regulating cell growth and function between the double-deck cell.The characteristics of co-culture device of the present invention are that the distance between two confluent monolayer cells can onlinely be regulated, realize the regulating effect of gap cell growth and function thereof, for co-culture of cells provides a kind of adjustable growing environment, provide a kind of method for exploring the co-culture of cells technology.
The above only is preferred embodiment of the present invention, is not limited to the present invention, and for a person skilled in the art, the present invention can have various changes and variation.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. the double-deck cell co-culture device of an adjustable gaps is characterized in that, comprising:
Cell culture case is used for cultivating first cell;
The cell cultures groove is used for cultivating second cell, and described cell cultures groove is removably set in the described cell culture case;
Driving part drives described cell cultures groove bottom to described cell culture case in described cell culture case and moves, with the gap of the bottom of the bottom of adjusting described cell culture case and described cell cultures groove to predetermined distance.
2. device as claimed in claim 1 is characterized in that, is formed with the spring plectrum of the described cell cultures groove of resiliency supported on the described cell culture case.
3. device as claimed in claim 2, described driving part is to be arranged on the sliding sash that can move along described cell culture case length direction on the described cell culture case and to be arranged on the push rod that described sliding sash moves that promotes on the described cell culture case, be formed with the inclined-plane at described cell cultures groove, described sliding sash be formed with described inclined-plane suitable be used for to promote described cell cultures groove to the mobile skewback in described cell culture case bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310093587.0A CN103194388B (en) | 2013-03-22 | 2013-03-22 | Double-layer cell co-culture device with adjustable gap |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310093587.0A CN103194388B (en) | 2013-03-22 | 2013-03-22 | Double-layer cell co-culture device with adjustable gap |
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| CN103194388A true CN103194388A (en) | 2013-07-10 |
| CN103194388B CN103194388B (en) | 2014-06-11 |
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| CN201310093587.0A Active CN103194388B (en) | 2013-03-22 | 2013-03-22 | Double-layer cell co-culture device with adjustable gap |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232485A (en) * | 2014-09-28 | 2014-12-24 | 浙江大学常州工业技术研究院 | Cell co-culture device |
| CN110804548A (en) * | 2019-11-20 | 2020-02-18 | 重庆医药高等专科学校 | Cell co-culture equipment |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1407853A (en) * | 1999-07-23 | 2003-04-02 | 耶路撒冷希伯来大学伊森姆研究发展公司 | Device and method for performing biological modification of fluid |
| CN1678909A (en) * | 2002-08-28 | 2005-10-05 | 法国原子能委员会 | Device for measuring the electrical activity of biological elements and its applications |
| CN202175680U (en) * | 2011-04-18 | 2012-03-28 | 北京航空航天大学 | Coculture double-channel mechanical force loading flow chamber device for in vitro cells |
-
2013
- 2013-03-22 CN CN201310093587.0A patent/CN103194388B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1407853A (en) * | 1999-07-23 | 2003-04-02 | 耶路撒冷希伯来大学伊森姆研究发展公司 | Device and method for performing biological modification of fluid |
| CN1678909A (en) * | 2002-08-28 | 2005-10-05 | 法国原子能委员会 | Device for measuring the electrical activity of biological elements and its applications |
| CN202175680U (en) * | 2011-04-18 | 2012-03-28 | 北京航空航天大学 | Coculture double-channel mechanical force loading flow chamber device for in vitro cells |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232485A (en) * | 2014-09-28 | 2014-12-24 | 浙江大学常州工业技术研究院 | Cell co-culture device |
| CN110804548A (en) * | 2019-11-20 | 2020-02-18 | 重庆医药高等专科学校 | Cell co-culture equipment |
| CN110804548B (en) * | 2019-11-20 | 2023-05-30 | 重庆医药高等专科学校 | Cell co-culture equipment |
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| CN103194388B (en) | 2014-06-11 |
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