CN220724199U

CN220724199U - Cell culture plate

Info

Publication number: CN220724199U
Application number: CN202321644496.7U
Authority: CN
Inventors: 郭浩; 王文静
Original assignee: Shandong Ouruopu Biotechnology Co ltd
Current assignee: Shandong Ouruopu Biotechnology Co ltd
Priority date: 2023-06-27
Filing date: 2023-06-27
Publication date: 2024-04-05
Anticipated expiration: 2033-06-27

Abstract

The utility model discloses a cell culture plate which comprises a culture seat, a culture tank, a culture cover and a connecting shaft, wherein the culture seat is movably connected with the culture cover through the connecting shaft, clamping grooves are formed in the top of the culture seat, the surface of the culture seat is provided with the connecting seat, the top of the culture cover is provided with the connecting cover, one side of the connecting seat is fixedly connected with the surface of the culture seat, the bottom of the connecting cover is fixedly connected with the top of the culture cover, a groove is formed in the top of the connecting seat, a fixed block is arranged on the surface of the connecting cover, the connecting cover is fixedly clamped with the inner wall of the groove of the connecting seat through the fixed block, sealing gaskets are arranged on the surface of the culture seat, the sealing gaskets are the same as the clamping grooves in number, the culture cover is pulled downwards, the culture cover is fixedly clamped with the culture seat at the moment, the sealing gaskets are positioned in the clamping grooves, each culture groove is separated through the sealing gaskets, and pollution caused by cell overflow in each culture groove is prevented.

Description

Cell culture plate

Technical Field

The utility model belongs to the technical field of cell culture, and particularly relates to a cell culture plate.

Background

The cell culture plate is a biological appliance and is mainly used for culturing cells in biological experiments, the cell culture plate can be divided into a flat bottom and a round bottom according to different bottom shapes, and different cell culture plates are selected according to different cultured cells.

In order to prevent the contamination of the bacteria during the cell culture, the operation is generally performed on the super clean bench, when the cell culture plate is required to be transferred to other devices and other positions after being processed on the super clean bench, the cover plate of the cell culture plate is required to be covered, and in order to ensure the sealing performance between the cell culture plate and the cover plate, sealing strips are usually arranged at the joints of the cell culture plate and the cover plate, so that the cell culture plate and the cover plate can generate elastic force during the sealing, the sealing strips or sealing strips are used for sealing, the tension of the sealing strips is limited, the tension is possibly smaller than the elastic force, the cover plate is provided with openings due to the elastic force of the sealing strips, the cell culture plate is directly exposed to the air and is contaminated by the bacteria, and in addition, when the single culture tank is operated, the whole plate cover is required to be opened, so that the culture plate is exposed to the outside in a large area, and the culture of other culture tanks is easy to be contaminated.

Disclosure of Invention

The present utility model provides a cell culture plate to solve the above-mentioned problems in the prior art.

In order to solve the technical problems, the utility model adopts the following technical scheme:

the utility model provides a cell culture board, includes culture dish, culture tank, cultivates lid and connecting axle, the bottom fixed mounting of culture tank is in the inside of culture dish, culture dish passes through connecting axle swing joint with the culture lid, the draw-in groove has been seted up at the top of culture dish, the surface of culture dish is equipped with the connecting seat, the top of cultivateing the lid is equipped with the connecting lid, one side of connecting seat and the fixed surface connection of culture dish, the bottom of connecting lid and culture lid top fixed connection, the recess has been seted up at the top of connecting seat, the surface of connecting lid is equipped with the fixed block, the connecting lid passes through the fixed block and is fixed with the recess inner wall joint of connecting seat, the culture dish surface is equipped with sealed pad, the quantity of sealed pad is the same with the quantity of draw-in groove, and is fixed with the culture dish joint of cultivateing the lid down, and sealed pad is in the draw-in groove inside this moment, separates each culture dish through sealed pad, prevents that the inside cell overflow of each culture dish from causing pollution.

The technical scheme of the utility model is further improved as follows: through the cooperation of connecting seat, connecting cover, draw-in groove and sealed pad, prevent that each inside cell of culture tank from overflowing and causing the pollution, cultivate the lid joint simultaneously and fix and can prevent that sealed pad from will cultivateing the lid bullet and open at the culture seat surface.

Adopt above-mentioned scheme, in this scheme cultivate the fixed surface of lid installs the sleeve pipe, sheathed tube top is equipped with sealed lid, sheathed tube inside is the fretwork state, sheathed tube top fixedly connected with spliced pole, the top of spliced pole and sealed bottom fixed connection of lid, the surface active mounting of spliced pole has the loose axle, the spout has been seted up to sealed inside, one side fixedly connected with fly leaf of loose axle, the fly leaf is transparent glass material, one side fixedly connected with push rod of fly leaf, one side of push rod is located the inside of spout, when needs carry out single culture tank liquid change, liquid feeding, makes the fly leaf control at the sleeve pipe top through rotating the push rod, and the sleeve pipe top is in the open state this moment, carries out single liquid change liquid feeding operation.

The technical scheme of the utility model is further improved as follows: through the cooperation of sleeve pipe, spliced pole, loose axle, spout, push rod and fly leaf, reached and to be able to trade liquid feeding operation to single culture tank, the culture plate is exposed in the external world in a large scale when avoiding trading the liquid.

By adopting the scheme, the bayonet is arranged on two sides of the fixed block in the scheme, the button is movably arranged at the bottom of the connecting seat, the top of the button is positioned in the connecting seat, the spring is fixedly connected to the bottom of the button, the top of the spring is fixedly connected to the inner wall of the connecting seat, the connecting seat is internally provided with the connecting rod, the movable shaft II, the connecting rod II and the spring II, one end of the connecting rod is movably connected with the top of the button through the movable shaft II, the top of the movable shaft II is movably connected with the tail of the connecting rod II, one side of the connecting rod II is fixedly connected with the spring II, one side of the spring II is fixedly connected with the clamping block, the clamping block is triangular, the diameter of the clamping block is equal to the inner diameter of the bayonet, when the fixed block moves downwards, the clamping block is inwards contracted through the spring, and is sprung up when positioned in the bayonet, so that the culturing seat is fixedly connected with the culturing cover, the button is pressed upwards, the top of the connecting rod II moves rightwards through the spring, and the clamping block is positioned outside the bayonet, and the culturing cover can be taken down from the surface of the culturing seat.

The technical scheme of the utility model is further improved as follows: through the cooperation of button, spring two, connecting rod two, fixture block and bayonet socket, reached the effect of conveniently connecting fixedly and taking off sealed lid.

By adopting the technical scheme, compared with the prior art, the utility model has the following technical progress:

1. the utility model provides a cell culture plate, which is characterized in that a culture cover is pulled downwards through the cooperation of a connecting seat, a connecting cover, a clamping groove and a sealing gasket, so that the culture cover is clamped and fixed with the culture seat, the sealing gasket is positioned in the clamping groove at the moment, each culture groove is separated by the sealing gasket to prevent pollution caused by cell overflow in each culture groove, meanwhile, the clamping and fixing of the culture cover on the surface of the culture seat can prevent the sealing gasket from bouncing the culture cover, and when liquid exchange and liquid adding of a single culture groove are needed, the movable plate is moved left and right at the top of the sleeve by rotating the push rod, the top of the sleeve is in an opening state at the moment, and the large-area exposure of the culture plate to the outside during liquid exchange is avoided.

2. The utility model provides a cell culture plate, which is characterized in that a button, a spring II, a connecting rod II, a clamping block and a bayonet are matched, when a fixed block moves downwards, the clamping block is inwards contracted through the spring and is sprung up when positioned in the bayonet, so that a culture seat and a culture cover are clamped and fixed, the button is upwards extruded, the top end of the connecting rod moves rightwards through inwards contracted spring, the connecting rod II moves rightwards simultaneously, and at the moment, the clamping block is positioned outside the bayonet, so that a sealing cover can be taken off from the surface of the sealing seat.

Drawings

FIG. 1 is a schematic perspective view of a culture plate according to the present utility model;

FIG. 2 is a schematic view showing a closed perspective structure of the culture plate of the present utility model;

FIG. 3 is a schematic cross-sectional view of a connector according to the present utility model;

FIG. 4 is a schematic view of a fly leaf attachment configuration of the present utility model;

in fig. 1-4: 1. a culture base; 11. a clamping groove; 2. a culture tank; 3. a culture cover; 31. a sealing gasket; 32. a sleeve; 33. sealing cover; 331. a chute; 34. a connecting column; 35. a movable shaft; 36. a movable plate; 37. a push rod; 4. a connecting seat; 41. a button; 42. a spring; 43. a connecting rod; 44. a movable shaft II; 45. a second connecting rod; 46. a second spring; 47. a clamping block; 5. a connection cover; 51. a fixed block; 52. a bayonet; 6. and a connecting shaft.

Detailed Description

In order to better understand the above technical solutions, the following detailed description will refer to the accompanying drawings and specific embodiments.

Example 1

As shown in fig. 1-4, a cell culture plate comprises a culture seat 1, a culture tank 2, a culture cover 3 and a connecting shaft 6, wherein the bottom of the culture tank 2 is fixedly arranged in the culture seat 1, the culture seat 1 and the culture cover 3 are movably connected through the connecting shaft 6, a clamping groove 11 is formed in the top of the culture seat 1, the surface of the culture seat 1 is provided with the connecting seat 4, the top of the culture cover 3 is provided with the connecting cover 5, one side of the connecting seat 4 is fixedly connected with the surface of the culture seat 1, the bottom of the connecting cover 5 is fixedly connected with the top of the culture cover 3, a groove is formed in the top of the connecting seat 4, a fixing block 51 is arranged on the surface of the connecting cover 5, the connecting cover 5 is fixedly clamped with the inner wall of the groove of the connecting seat 4 through the fixing block 51, the number of the sealing gaskets 31 is the same as that of the clamping grooves 11, the culture cover 3 is pulled downwards, the culture cover 3 is fixedly clamped with the culture seat 1, the sealing gaskets 31 are positioned in the clamping groove 11 at the moment, each culture tank 2 is separated through the sealing gaskets 31, and cell overflow inside each culture tank 2 is prevented.

Example 2

As shown in fig. 1-4, on the basis of embodiment 1, the surface of the culture cover 3 is fixedly provided with the sleeve 32, the top of the sleeve 32 is provided with the sealing cover 33, the inside of the sleeve 32 is in a hollowed-out state, the top of the sleeve 32 is fixedly connected with the connecting column 34, the top of the connecting column 34 is fixedly connected with the bottom of the sealing cover 33, the surface of the connecting column 34 is movably provided with the movable shaft 35, the inside of the sealing cover 33 is provided with the sliding groove 331, one side of the movable shaft 35 is fixedly connected with the movable plate 36, the movable plate 36 is made of transparent glass, one side of the movable plate 36 is fixedly connected with the push rod 37, one side of the push rod 37 is positioned in the sliding groove 331, when the liquid is required to be changed and fed in the single culture tank 2, the movable plate 36 is moved left and right at the top of the sleeve 32, at the moment, the top of the sleeve 32 is in an opening state, and the single liquid changing operation is performed, and the large area of the culture plate is prevented from being exposed to the outside during the liquid changing.

Example 3

As shown in fig. 1-4, on the basis of embodiment 1, bayonet 52 is opened at both sides of fixed block 51, bottom movable mounting of connecting seat 4 has button 41, the top of button 41 is located the inside of connecting seat 4, the bottom fixedly connected with spring 42 of button 41, the top fixed connection of spring 42 is at the inner wall of connecting seat 4, the inside of connecting seat 4 is equipped with connecting rod 43, loose axle two 44, connecting rod two 45 and spring two 46, the one end of connecting rod 43 passes through loose axle two 44 swing joint with the top of button 41, the top of loose axle two 44 and the afterbody swing joint of connecting rod two 45, one side fixed connection spring two 46 has fixture block 47, fixture block 47 is triangle-shaped, and the diameter of fixture block 47 equals with the inside diameter length of bayonet 52, when fixed block 51 moves down, spring 47 is through spring two 46 inwards shrink, when being located the inside of bayonet 52, make culture seat 1 and culture lid 3 joint fixed, upwards extrude button 41, inwards shrink through spring 42, connecting rod 43 top right fixture block moves, connecting rod two 45 moves right, at the same time, fixture block 47 is located on the outside of culture seat 1, can be taken down from the outside surface 3.

The following is a specific description of the cell culture plate.

As shown in fig. 1-4, cells to be cultured are dripped into the culture tanks 2, then the culture cover 3 is pulled downwards, so that the culture cover 3 is clamped and fixed with the culture seat 1, the sealing gasket 31 is positioned in the clamping groove 11 at the moment, each culture tank 2 is separated by the sealing gasket 31, pollution caused by cell overflow in each culture tank 2 is prevented, when liquid and liquid are required to be replaced and fed in the single culture tank 2, the movable plate 36 is moved left and right at the top of the sleeve 32 by rotating the push rod 37, at the moment, the top of the sleeve 32 is in an opening state, single liquid and liquid feeding operation is performed, the large-area exposure of the culture plate in the outside during liquid replacement is avoided, the clamping block 47 is contracted inwards by the spring II 46 when the fixed block 51 moves downwards, the culture seat 1 is clamped and fixed with the culture cover 3, the button 41 is pressed upwards, the top end of the connecting rod 43 is contracted inwards by the spring 42, the clamping block II 45 is moved rightwards at the moment, and the clamping block 47 is positioned outside the clamping block 52, so that the culture cover 3 can be removed from the surface of the culture seat 1.

The foregoing utility model has been generally described in great detail, but it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, it is intended to cover modifications or improvements within the spirit of the inventive concepts.

Claims

1. Cell culture board, including culture dish (1), culture tank (2), cultivation lid (3) and connecting axle (6), its characterized in that: the bottom fixed mounting of culture tank (2) is in the inside of cultivateing seat (1), cultivate seat (1) and cultivate lid (3) through connecting axle (6) swing joint, draw-in groove (11) have been seted up at the top of cultivateing seat (1), the surface of cultivateing seat (1) is equipped with connecting seat (4), the top of cultivateing lid (3) is equipped with connecting cover (5), one side of connecting seat (4) and the fixed surface of cultivateing seat (1) are connected, the bottom of connecting cover (5) and the fixed surface of cultivateing lid (3) are connected, the recess has been seted up at the top of connecting seat (4), the surface of connecting cover (5) is equipped with fixed block (51), the connecting cover (5) is fixed through the recess inner wall joint of fixed block (51) and connecting seat (4), the surface of cultivateing seat (1) is equipped with sealed pad (31), the quantity of sealed pad (31) is the same with the quantity of draw-in groove (11).

2. A cell culture plate according to claim 1, wherein: the surface fixed mounting of cultivateing lid (3) has sleeve pipe (32), the top of sleeve pipe (32) is equipped with sealed lid (33), the inside of sleeve pipe (32) is the fretwork state, the top fixedly connected with spliced pole (34) of sleeve pipe (32), the top of spliced pole (34) and the bottom fixed connection of sealed lid (33), the surface movable mounting of spliced pole (34) has loose axle (35).

3. A cell culture plate according to claim 2, wherein: the inside of sealed lid (33) has seted up spout (331), one side fixedly connected with fly leaf (36) of loose axle (35), fly leaf (36) are transparent glass material, one side fixedly connected with push rod (37) of fly leaf (36), one side of push rod (37) is located the inside of spout (331).

4. A cell culture plate according to claim 1, wherein: bayonet sockets (52) are formed in two sides of the fixed block (51), a button (41) is movably mounted at the bottom of the connecting seat (4), the top of the button (41) is located inside the connecting seat (4), a spring (42) is fixedly connected to the bottom of the button (41), the top of the spring (42) is fixedly connected to the inner wall of the connecting seat (4), and a connecting rod (43), a movable shaft II (44), a connecting rod II (45) and a spring II (46) are arranged inside the connecting seat (4).

5. A cell culture plate according to claim 4, wherein: one end of the connecting rod (43) is movably connected with the top of the button (41) through a movable shaft II (44), the top of the movable shaft II (44) is movably connected with the tail of the connecting rod II (45), one side of the connecting rod II (45) is fixedly connected with a spring II (46), one side of the spring II (46) is fixedly connected with a clamping block (47), the clamping block (47) is triangular, and the diameter of the clamping block (47) is equal to the length of the inner diameter of the bayonet (52).