CN214300193U

CN214300193U - Novel suspension cell culture bottle

Info

Publication number: CN214300193U
Application number: CN202023116139.2U
Authority: CN
Inventors: 孙旭燕; 张学娟; 高宗圣; 王帅; 刁小娟; 孙健; 宋芳; 张文丽; 马鑫鑫
Original assignee: Shandong Qilu Stem Cell Engineering Co ltd
Current assignee: Shandong Qilu Stem Cell Engineering Co ltd
Priority date: 2020-12-22
Filing date: 2020-12-22
Publication date: 2021-09-28
Anticipated expiration: 2030-12-22

Abstract

The utility model discloses a novel suspension cell culture bottle, including bottle, body, bottle top, filtration and filter tube, body upper end and bottle top fixed connection, and the upper right side bottleneck is installed on the bottle top, and the upper left side of bottle top installs the left side bottleneck, and left side bottleneck tip spiro union has the liquid lid of getting simultaneously to right side bottleneck tip spiro union has the liquid lid of trading, filter tube fixed connection is on the inside right side in bottle top, and the inside fixed chamber of having seted up of filter tube, and the installation cavity has been seted up to the filter tube bottom simultaneously to the inside fixedly connected with nylon filter screen in installation cavity. According to the novel suspension cell culture bottle, the nylon filter screen is fixedly connected to the lower end of the filter pipe at the bottom of the right bottleneck, waste liquid can be directly poured out through the nylon filter screen when suspension cell liquid is changed, cells are left in the bottle, and then fresh cell culture liquid is directly supplemented, so that the cell centrifugation process is omitted, the loss of the cells is avoided, and the operation time is saved; the experiment cost is reduced, and the experiment time is shortened.

Description

Novel suspension cell culture bottle

Technical Field

The utility model relates to a cell culture equipment science and technology technical field specifically is a novel suspension cell culture bottle.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function; cell types are classified into an adhesion type and a suspension type according to the characteristics of growth on the support; suspension type cells grow without attaching to a support, the cell bodies are circular, the growth space in a culture solution is large, the cells can grow for a long time, and the cells can propagate vigorously, so that the solution is changed for 2-3 days in the suspension cell culture process, the main solution changing method is centrifugation solution changing at present, and the centrifugation solution changing has the following defects:

1. in the process of centrifugal liquid change, partial cells are lost in the processes of suction by a suction tube, centrifugation by a centrifuge tube and the like, and the centrifugation can cause certain damage to the cells, so that the growth of the cells is slowed down, and the experimental efficiency and the success rate are reduced;

2. the centrifugal liquid changing process needs to repeatedly enter and exit the sterile operating platform, so that the risk of cell pollution is increased, and the experiment progress is influenced;

3. the centrifugation liquid changing process needs to use experimental consumables such as centrifuge tubes, so that the experimental cost is increased, and the experimental time is prolonged.

In view of the above drawbacks, the present invention has been made by intensive studies.

Therefore, we propose a novel suspension cell culture flask to solve the above problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a novel suspension cell culture bottle to solve the defect mentioned in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a novel suspension cell culture bottle, includes bottle, body, bottle top, filtration and filter tube, body upper end and bottle top fixed connection, and the upper right side of bottle top installs the right side bottleneck, and the upper left side of bottle top installs the left side bottleneck, and left side bottleneck tip spiro union has the liquid lid of getting simultaneously to right side bottleneck tip spiro union has the liquid lid of trading, filter tube fixed connection is on the inside right side of bottle top, and the inside fixed chamber of having seted up of filter tube, and the installation cavity has been seted up to the filter tube bottom simultaneously to the inside fixedly connected with nylon filter screen of installation cavity.

Preferably, the bottle body is composed of a bottle body, a bottle top, a left side bottle neck and a right side bottle neck, and the bottle body is of a rectangular structure made of a polypropylene material.

Preferably, the nylon filter screen is concentric circle structure with the filter tube, and the aperture of nylon filter screen is 1 ~ 2 um.

Preferably, the slope installation end has all been seted up to the both sides of bottle top, and two sets of slope installation end surfaces have been seted up respectively and have been got the liquid mouth and trade the liquid mouth, get the liquid mouth and trade the inside left side bottleneck and the right side bottleneck of installing respectively of liquid mouth.

Preferably, the left side bottleneck and the right side bottleneck are the slope setting, and the contained angle between left side bottleneck and the bottle lateral wall is 120, and the contained angle between right side bottleneck and the bottle lateral wall is 120 simultaneously.

Preferably, the liquid changing cover and the liquid taking cover are hydrophobic and breathable bottle caps, and the end parts of the liquid changing cover and the liquid taking cover on the right bottle neck and the left bottle neck are of detachable structures.

Preferably, the filtering structure is composed of a nylon filter screen and a filtering pipe, a cavity is arranged inside the bottle body, the cavity is communicated with the right bottle neck through the nylon filter screen, the fixed cavity and the liquid exchange port, and the lower end of the right bottle neck is in threaded connection with the surface of the inner wall of the fixed cavity.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the right bottle neck and the left bottle neck are both arranged obliquely to the bottle body; due to the design, when the bottle body is horizontally placed, the positions of the right bottle neck and the left bottle neck are higher, and the culture medium in the bottle body cannot overflow when more culture medium exists;

2. the nylon filter screen is fixedly connected to the lower end of the filter pipe at the bottom of the right bottleneck, waste liquid can be directly poured out through the nylon filter screen when the suspension cell is changed, cells are left in the bottle, and then fresh cell culture solution is directly supplemented, so that the cell centrifugation process is omitted, the loss of the cells is avoided, and the operation time is saved; the experiment cost is reduced, and the experiment time is shortened.

Drawings

FIG. 1 is a front view of the structure of the present invention;

fig. 2 is a schematic side plan view of the structure of fig. 1 of the present invention;

FIG. 3 is a schematic view of the internal structure of the bottle top of the present invention;

fig. 4 is the cross-sectional view of the nylon filter net and the filter tube of the present invention.

Reference numbers in the figures: 1. a bottle body; 2. a bottle body; 3. a liquid changing cover; 4. a right bottleneck; 5. bottle top; 6. a liquid taking cover; 7. a left bottleneck; 8. a liquid taking port; 9. a cavity; 10. a filter structure; 11. a nylon filter screen; 12. a mounting cavity; 13. a filter tube; 14. an inclined mounting end; 15. a liquid changing port; 16. and fixing the cavity.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: a novel suspension cell culture bottle comprises a bottle body 1, a bottle body 2, a bottle top 5, a filtering structure 10 and a filtering pipe 13, wherein the upper end of the bottle body 2 is fixedly connected with the bottle top 5, a right side bottleneck 4 is installed on the right upper side of the bottle top 5, a left side bottleneck 7 is installed on the left upper side of the bottle top 5, a liquid taking cover 6 is screwed on the end part of the left side bottleneck 7, a liquid changing cover 3 is screwed on the end part of the right side bottleneck 4, the liquid changing cover 3 and the liquid taking cover 6 are both hydrophobic and breathable bottle caps, and the liquid changing cover 3 and the liquid taking cover 6 are of detachable structures at the end parts of the right side bottleneck 4 and the left side bottleneck 7; the filter pipe 13 is fixedly connected to the right side inside the bottle top 5, a fixed cavity 16 is formed inside the filter pipe 13, an installation cavity 12 is formed in the bottom of the filter pipe 13, and a nylon filter screen 11 is fixedly connected inside the installation cavity 12; the nylon filter screen 11 and the filter pipe 13 are of concentric circle structures, and the aperture of the nylon filter screen 11 is 1-2 um; the bottle body 1 consists of four parts, namely a bottle body 2, a bottle top 5, a left bottle neck 7 and a right bottle neck 4, and the bottle body 1 is of a rectangular structure made of a polypropylene material; the two sides of the bottle top 5 are both provided with inclined mounting ends 14, the surfaces of the two groups of inclined mounting ends 14 are respectively provided with a liquid taking port 8 and a liquid changing port 15, and a left bottle neck 7 and a right bottle neck 4 are respectively arranged in the liquid taking port 8 and the liquid changing port 15; the left bottle neck 7 and the right bottle neck 4 are obliquely arranged, the included angle between the left bottle neck 7 and the side wall of the bottle body 1 is 120 degrees, and the included angle between the right bottle neck 4 and the side wall of the bottle body 1 is 120 degrees; the filtering structure 10 is composed of a nylon filter screen 11 and a filtering pipe 13, a cavity 9 is arranged inside the bottle body 1, the cavity 9 is communicated with the right bottle neck 4 through the nylon filter screen 11, a fixed cavity 16 and a liquid changing port 15, and the lower end of the right bottle neck 4 is screwed on the surface of the inner wall of the fixed cavity 16.

As shown in fig. 3: the nylon filter screen 11 is fixedly connected to the lower end of the filter pipe 13 at the bottom of the right bottleneck 4, waste liquid can be directly poured out through the nylon filter screen 11 when the suspension cell is changed, cells are left in the bottle, and then fresh cell culture solution is directly supplemented, so that the cell centrifugation process is omitted, the loss of the cells is avoided, and the operation time is saved; the aperture of the nylon filter screen 11 is 1 um-2 um; by the design, the cell waste liquid can be ensured to flow out smoothly, and all cells can be remained in the cell bottle.

As shown in fig. 1-2: the right bottle neck 4 and the left bottle neck 7 are both arranged obliquely to the bottle body 1; so design, when bottle 1 is kept flat, the position of right side bottleneck 4 and left side bottleneck 7 is higher, also can not overflow when the culture medium in the bottle 1 is more, still conveniently draws the cell simultaneously from the blake bottle, adds or abandons the culture medium.

The working principle is as follows: when the novel suspension cell culture bottle is used, the left liquid taking cover 6 is opened, cell suspension is added into the bottle body 1 from the left bottle neck 7, the liquid taking cover 6 is screwed, and the bottle body is placed into a carbon dioxide incubator for culture; when the culture solution needs to be replaced, the solution replacing cover 3 is opened, the waste solution is poured out from the right bottleneck 4, the cells are remained in the bottle body 1 because the diameter of the suspended cells is larger than the aperture of the nylon filter screen 11, and then the new culture solution is added from the left bottleneck 7, so that the process of centrifugal solution replacing is omitted; when the sampling or the cell harvesting is needed, the liquid taking cover 6 is opened, and the sampling is needed; the liquid changing cover 3 and the liquid taking cover 6 are arranged, so that cell culture liquid can be conveniently changed, the risk of cell pollution and cell loss is reduced, the liquid changing cover 3 and the liquid taking cover 6 are both hydrophobic and air-permeable bottle covers, the air permeability of cell culture is increased, and the cell culture efficiency is improved; this is the whole process of the work of the novel suspension cell culture flask.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a novel suspension cell culture bottle, includes bottle (1), body (2), bottle top (5), filtration (10) and filter tube (13), its characterized in that: body (2) upper end and bottle top (5) fixed connection, and bottle top (5) upper right side installs right side bottleneck (4), and left side bottleneck (7) are installed to the upper left side of bottle top (5), and left side bottleneck (7) tip spiro union has liquid lid (6) of getting simultaneously to right side bottleneck (4) tip spiro union has liquid lid (3) of trading, filter tube (13) fixed connection is on the inside right side of bottle top (5), and filter tube (13) inside has seted up fixed chamber (16), and installation cavity (12) have been seted up to filter tube (13) bottom simultaneously to installation cavity (12) inside fixedly connected with nylon filter screen (11).

2. The novel suspension cell culture flask of claim 1, wherein: the bottle body (1) is composed of a bottle body (2), a bottle top (5), a left side bottle neck (7) and a right side bottle neck (4), and the bottle body (1) is of a rectangular structure made of a polypropylene material.

3. The novel suspension cell culture flask of claim 1, wherein: nylon filter screen (11) are concentric circle structure with filter tube (13), and the aperture of nylon filter screen (11) is 1 ~ 2 um.

4. The novel suspension cell culture flask of claim 1, wherein: the both sides of bottle top (5) have all been seted up slope installation end (14), and two sets of slope installation end (14) surfaces have been seted up respectively and have been got liquid mouth (8) and trade liquid mouth (15), get liquid mouth (8) and trade liquid mouth (15) inside and install left side bottleneck (7) and right side bottleneck (4) respectively.

5. The novel suspension cell culture flask of claim 1, wherein: left side bottleneck (7) and right side bottleneck (4) are the slope setting, and the contained angle between left side bottleneck (7) and bottle (1) lateral wall is 120, and the contained angle between right side bottleneck (4) and bottle (1) lateral wall is 120 simultaneously.

6. The novel suspension cell culture flask of claim 1, wherein: the liquid changing cover (3) and the liquid taking cover (6) are hydrophobic and breathable bottle caps, and the liquid changing cover (3) and the liquid taking cover (6) are of detachable structures at the end parts of a right bottle neck (4) and a left bottle neck (7).

7. The novel suspension cell culture flask of claim 1, wherein: the filter structure (10) comprises a nylon filter screen (11) and a filter pipe (13), a cavity (9) is arranged inside the bottle body (1), the cavity (9) is communicated with the right bottle neck (4) through the nylon filter screen (11), a fixed cavity (16) and a liquid changing port (15), and the lower end of the right bottle neck (4) is screwed on the inner wall surface of the fixed cavity (16).