CN220335212U - Organ-like culture device - Google Patents
Organ-like culture device Download PDFInfo
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- CN220335212U CN220335212U CN202320979563.4U CN202320979563U CN220335212U CN 220335212 U CN220335212 U CN 220335212U CN 202320979563 U CN202320979563 U CN 202320979563U CN 220335212 U CN220335212 U CN 220335212U
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- culture
- organoid
- box body
- culture tank
- culture device
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- 210000002220 organoid Anatomy 0.000 claims abstract description 65
- 239000000463 material Substances 0.000 claims description 14
- 238000009434 installation Methods 0.000 claims description 10
- 210000000056 organ Anatomy 0.000 claims description 5
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- 239000002982 water resistant material Substances 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 5
- 238000005192 partition Methods 0.000 abstract description 4
- 239000012531 culture fluid Substances 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 46
- 238000000034 method Methods 0.000 description 11
- 238000004113 cell culture Methods 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000004927 fusion Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
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- 238000011156 evaluation Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
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- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- 230000001988 toxicity Effects 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
The utility model discloses an organoid culture device which comprises a box body, a culture tank and a cover plate, wherein the cover plate is detachably covered on a box opening of the box body, a plurality of partition boards are arranged in the box body, an inner cavity of the box body is divided into a plurality of mounting cavities through the partition boards, the culture tank is detachably mounted in the mounting cavities, an insert is arranged in the culture tank, a plurality of small chambers with upper ends open are arranged in the insert, a plurality of first small holes and second small holes are respectively formed in the chamber wall of the small chambers and the outer wall of the insert, and the diameters of the first small holes and the second small holes are smaller than the diameter of organoids to be cultured. The utility model provides an organoid culture device, wherein an insert is arranged in a culture tank, culture fluid can be freely exchanged through a first small hole on the wall of a small chamber, fresh culture fluid can be exchanged through the first small hole, the operation is convenient, and normal development and growth of organoids can be ensured.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to an organoid culture device.
Background
Organoids are a "micro-organ" formed by stem cells cultured in an in vitro environment, which contain a variety of tissue-specific cell types and self-organize into three-dimensional tissue structures and can partially mimic the structure and function of the native organ. At present, organoids are widely applied to the fields of toxicity detection, drug effect evaluation and screening, disease model establishment and research on genetic diseases, infectious diseases, cancers and the like of drugs.
Organoid culture techniques are an important group of organoids. At present, organoid culture techniques are mainly classified into three-dimensional culture techniques that rely on exogenous scaffolds and three-dimensional culture techniques that do not rely on exogenous scaffolds. The three-dimensional culture which does not depend on an exogenous bracket is a simple and convenient cell culture method, and the cell bracket does not need to be additionally added, but a cell culture vessel treated by a cell rejection material or a special system such as hanging drops, microfluidics and the like is used for carrying out cell culture, so that the attachment of cells on the surface of the vessel is prevented, and the cells are forced to be aggregated to form cell microspheres. The 3D cell model obtained by the method has a complete sphere structure, can spontaneously form an extracellular matrix, and well simulate oxygen and nutrition gradients in an in-vivo environment.
The low adhesion surface method independent of the exogenous scaffold is the most common method of the organoid culture technology because of the convenience of operation, but cells which fall off during organoid differentiation are not dispersed during culture, are still in close contact with or around the organoid, and can adhere to the organoid, possibly affecting the normal development and growth of the organoid. Meanwhile, in the process of liquid exchange operation, the damage of a three-dimensional structure is easy to cause.
Disclosure of Invention
In view of the defects existing at present, the utility model provides the organoid culture device, wherein the plug-in is arranged in the culture tank, the culture solution can be freely exchanged through the first small hole on the wall of the small chamber, and fresh culture solution can be exchanged through the first small hole, so that the organoid culture device is convenient to operate and can ensure normal development and growth of the organoid.
In order to achieve the above purpose, the embodiment of the present utility model adopts the following technical scheme:
the utility model provides a kind organ culture apparatus, includes box body, culture tank and apron, the apron can dismantle the lid in the box mouth of box body, the inside a plurality of baffles that are equipped with of box body, the inner chamber of box body passes through the baffle separates into a plurality of installation chambeies, installation intracavity demountable installation the culture tank, the inside plug-in components that set up of culture tank, the inside cell that includes a plurality of upper end open-ended of plug-in components, the outer wall of the chamber wall of cell and plug-in components sets up a plurality of first aperture and second aperture respectively, the diameter of first aperture and second aperture is less than the diameter of waiting to cultivate kind organ.
According to one aspect of the utility model, a first clamping hole is formed in the side wall of the mounting cavity, a first clamping block is arranged on the outer wall of the culture tank, and the first clamping block is clamped in the first clamping hole.
According to one aspect of the utility model, a second clamping block is arranged on the cover plate, a corresponding second clamping hole is formed in the box body, and the second clamping block is clamped in the second clamping hole.
According to one aspect of the utility model, the chamber is open at both ends, and the opening at the lower end communicates with the culture tank.
According to one aspect of the utility model, the insert is a low adhesion material.
According to one aspect of the utility model, the low adhesion material is polystyrene.
According to one aspect of the utility model, the culture tank, the box body, the cover plate and the plug-in unit are all made of light-transmitting materials.
According to one aspect of the utility model, the walls of the cells are of a water barrier material.
The implementation of the utility model has the advantages that: the utility model provides a organoid culture apparatus, the apron detachable lid in the box mouth of box body, the dismouting of being convenient for plays sealed box body's effect simultaneously, and then guarantees organoid spheroid culture process's airtight environment and prevents that it from being polluted. The inside plug-in components that set up of culture tank, the inside cell that includes a plurality of upper end open-ended of plug-in components, the outer wall of the chamber wall of cell and plug-in components sets up a plurality of first aperture and second aperture respectively, the diameter of first aperture and second aperture is less than the diameter of waiting to cultivate organoid, and the culture solution can realize the free exchange of culture solution through the first aperture on the cell pore wall, and can change fresh culture solution through first aperture, and it is more convenient to operate, saves time. Meanwhile, the organoid cell mass cannot pass through the first small hole and the second small hole, and the single organoid cell is singly placed in a small chamber, so that the fusion of the organoid cell can be effectively blocked. And small molecules, cells and the like which fall off during the differentiation of the organoids can pass through the first small hole and the second small hole and are sucked away when the new culture solution is replaced, so that the organoids can be ensured to develop and grow normally.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of an organoid culture device according to the present utility model;
FIG. 2 is an exploded view of an organoid culture device according to the present utility model;
FIG. 3 is a schematic illustration of the structure of an insert for an organoid culture device according to the present utility model;
FIG. 4 is a schematic diagram showing the cell structure of an organoid culture device according to the present utility model.
In fig. 1-4, 1, a box body; 2. a cover plate; 3. a culture tank; 4. an insert; 5. a mounting cavity; 6. a second clamping block; 7. a first clamping block; 8. a cell; 9. a first aperture; 10. and a second aperture.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in fig. 1, fig. 2, fig. 3 and fig. 4, an organoid culture device comprises a box body 1, a culture tank 3 and a cover plate 2, wherein the cover plate 2 is detachably covered on a box opening of the box body 1, a plurality of partition boards are arranged inside the box body 1, an inner cavity of the box body 1 is divided into a plurality of installation cavities 5 through the partition boards, the culture tank 3 is detachably installed in the installation cavities 5, an insert 4 is arranged inside the culture tank 3, a small chamber 8 with a plurality of upper ends open is arranged inside the insert 4, a plurality of first small holes 9 and second small holes 10 are respectively arranged on a chamber wall of the small chamber 8 and an outer wall of the insert 4, and diameters of the first small holes 9 and the second small holes 10 are smaller than diameters of organoids to be cultured.
In practical applications, the number of the cells 8 in the insert 4 may be determined by the sizes of the culture tanks 3, and the insert 4 including different numbers of the cells 8 may be designed according to the area sizes of the culture tanks 3. When in use, the cover plate 2 is opened to take out the culture tank 3, the plug-in 4 is inserted into the culture tank 3, then the organoids are transferred into the small chamber 8 for culture, and then the culture tank 3 is put into the box body 1, and the cover plate 2 is covered to ensure that the seal is not interfered by the external environment. And simultaneously, a worker observes the growth and development states of the organoids through a microscope. Adjacent cells 8 communicate through first apertures 9 in the chamber walls, allowing the culture fluid to freely exchange nutrient molecules within each cell 8. Because the diameters of the first small hole 9 and the second small hole 10 are smaller than those of the organoid cells, organoid cell clusters cannot pass through the first small hole 9 and the second small hole 10, and the organoid cells are singly placed in the small chamber 8, so that fusion of the organoid cells can be effectively blocked. And small molecules, cells and the like which fall off during the differentiation of the organoids can pass through the first small hole 9 and the second small hole 10 and are sucked away when a new culture solution is replaced, so that the organoids can be ensured to develop and grow normally. When fresh culture solution needs to be replaced, a worker takes out the culture tank 3, inclines the culture tank 3, waits for liquid in all the cells 8 to be gathered to the lowest position, and can suck old culture solution and shed cells through a pipetting gun, so that the time for replacing fresh culture solution is saved, and meanwhile, the shed cells can be prevented from adhering to the periphery of organoids to influence the development and growth of the organoids.
In practical application, because in the organoid culture environment, the bottom of the culture tank 3 is generally prepared from a low adsorption material, and is used for ensuring that organoid cells can grow in suspension in liquid, even if the organoid cells sink to the bottom of the culture dish, the organoid cell clusters are ensured not to be adsorbed to the bottom of the culture dish, and then the organoid cells are prevented from growing on the wall. Therefore, the lower end of the chamber 8 may be designed to be opened or the lower end of the chamber 8 may be designed to be closed, and the opening is communicated with the culture tank 3 when the lower end of the chamber 8 is designed to be opened. When the lower end of the chamber 8 is closed, the bottom is made of a low adsorption material, which also prevents organoid cells from adsorbing to the bottom of the chamber 8.
In practical application, be provided with first joint hole on the lateral wall of installation cavity 5, be provided with first fixture block 7 on the outer wall of culture tank 3, first fixture block 7 joint in the first joint hole to make culture tank 3 pass through the mode of joint and dismantle fixed mounting and be in on the installation cavity 5, thereby guarantee culture tank 3 stability in the use. The cover plate 2 is provided with a second clamping block 6, the box body 1 is provided with a corresponding second clamping hole, the second clamping block 6 is clamped in the second clamping hole, the cover plate 2 is detachably connected to the box body 1 in a clamping manner, so that the disassembly and assembly are convenient, the box body 1 is sealed, and the sealed environment of the organoid ball culturing process can be guaranteed and prevented from being polluted.
In practice, the insert 4 is of a low adhesion material. The low adhesion material may be selected from polystyrene.
In practical application, the walls of the cells 8 are made of water-proof materials, and nutrient substances can be exchanged between adjacent cells 8 through the first small holes 9.
In practical application, the culture tank 3, the box body 1, the cover plate 2 and the plug-in components 4 are all made of light-transmitting materials, so that the growth conditions of the organoids can be observed and recorded conveniently.
The implementation of the utility model has the advantages that: the utility model provides a organoid culture apparatus, the apron detachable lid in the box mouth of box body, the dismouting of being convenient for plays sealed box body's effect simultaneously, and then guarantees organoid spheroid culture process's airtight environment and prevents that it from being polluted. The inside plug-in components that set up of culture tank, the inside cell that includes a plurality of upper end open-ended of plug-in components, the outer wall of the chamber wall of cell and plug-in components sets up a plurality of first aperture and second aperture respectively, the diameter of first aperture and second aperture is less than the diameter of waiting to cultivate organoid, and the culture solution can realize the free exchange of culture solution through the first aperture on the cell pore wall, and can change fresh culture solution through first aperture, and it is more convenient to operate, saves time. Meanwhile, the organoid cell mass cannot pass through the first small hole and the second small hole, and the single organoid cell is singly placed in a small chamber, so that the fusion of the organoid cell can be effectively blocked. And small molecules, cells and the like which fall off during the differentiation of the organoids can pass through the first small hole and the second small hole and are sucked away when the new culture solution is replaced, so that the organoids can be ensured to develop and grow normally.
The foregoing is merely illustrative of the present utility model, and the present utility model is not limited thereto, and any changes or substitutions easily contemplated by those skilled in the art within the technical scope of the present utility model should be included in the scope of the present utility model. Therefore, the protection scope of the present utility model shall be subject to the protection scope of the claims.
Claims (8)
1. The utility model provides a kind organ culture apparatus, includes box body, culture tank and apron, the apron can dismantle the lid in the box mouth of box body, its characterized in that, the inside a plurality of baffles that are equipped with of box body, the inner chamber of box body passes through the baffle separates into a plurality of installation chambeies, installation intracavity demountable installation the culture tank, the inside plug-in components that set up of culture tank, the inside cell that includes a plurality of upper end open-ended of plug-in components, the outer wall of the chamber wall of cell and plug-in components sets up a plurality of first aperture and second aperture respectively, the diameter of first aperture and second aperture is less than the diameter of waiting to cultivate kind organ.
2. The organoid culture device of claim 1, wherein a first clamping hole is provided in a side wall of the mounting cavity, and a first clamping block is provided in an outer wall of the culture tank, and the first clamping block is clamped in the first clamping hole.
3. The organoid culture device of claim 2, wherein a second clamping block is provided on the cover plate, and a corresponding second clamping hole is provided on the box body, and the second clamping block is clamped in the second clamping hole.
4. The organoid culture device of claim 1 wherein the chamber is open at both ends and the opening at the lower end communicates with the culture tank.
5. The organoid culture device of claim 1 wherein the insert is a low adhesion material.
6. The organoid culture device of claim 5 wherein the low adhesion material is polystyrene.
7. The organoid culture device of claim 1 wherein the culture tank, cassette, cover and insert are all light transmissive materials.
8. The organoid culture device of claim 1 wherein the walls of the chamber are of a water-resistant material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320979563.4U CN220335212U (en) | 2023-04-26 | 2023-04-26 | Organ-like culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320979563.4U CN220335212U (en) | 2023-04-26 | 2023-04-26 | Organ-like culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220335212U true CN220335212U (en) | 2024-01-12 |
Family
ID=89447785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320979563.4U Active CN220335212U (en) | 2023-04-26 | 2023-04-26 | Organ-like culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220335212U (en) |
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2023
- 2023-04-26 CN CN202320979563.4U patent/CN220335212U/en active Active
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