CN208694541U - A kind of high efficiency cell culture bottle air filter and Tissue Culture Flask - Google Patents

A kind of high efficiency cell culture bottle air filter and Tissue Culture Flask Download PDF

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CN208694541U
CN208694541U CN201820972964.6U CN201820972964U CN208694541U CN 208694541 U CN208694541 U CN 208694541U CN 201820972964 U CN201820972964 U CN 201820972964U CN 208694541 U CN208694541 U CN 208694541U
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filter
filter membrane
membrane
concave station
cell culture
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CN201820972964.6U
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章新道
康安锋
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Cage (xiamen) Biotechnology Co Ltd
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Cage (xiamen) Biotechnology Co Ltd
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Abstract

The utility model provides a kind of high efficiency cell culture bottle air filter and Tissue Culture Flask, wherein high efficiency cell culture bottle air filter, including lid and filter body;Embedded hole is equipped at the top of lid;Embedded hole is connected with concave station;Concave station is equipped with opening;The inner cavity of concave station and lid passes through open communication;Filter body is equipped with the first filter membrane, the second filter membrane, third filter membrane and the 4th filter membrane;Filter core intracoelomic cavity is equipped with demarcation plate, so that the first filter membrane is connected to third filter membrane, the second filter membrane is connected to the 4th filter membrane;Filter body is on concave station;Filter body top matches with embedded hole;End face with the 4th filter membrane matches with opening;Gap is respectively kept between concave station and the second filter membrane and third filter membrane.The Tissue Culture Flask air filter increases gas and flows stroke, so that air filtration is more thorough by the cooperation of filter body and concave station;In addition, filter body is equipped with level Four filter membrane, air passes through multistage filtering, and clean-up effect becomes apparent from, is more efficient.

Description

A kind of high efficiency cell culture bottle air filter and Tissue Culture Flask
Technical field
The utility model relates to cell cultivation equipment, in particular to a kind of high efficiency cell culture bottle air filter and thin Born of the same parents' culture bottle.
Background technique
Cell culture refers to all in vitro cultures, and meaning, which refers to, takes out tissue in driven object living body body, in analogue body Physiological environment etc. it is specific in vivo under the conditions of, carry out incubation culture, be allowed to survive and grow.Cell culture work is answered extensively For every field such as biology, medicine, new drug developments, become one of most important basic science.Medicine and biology in China It learns and container of the vial as cell culture is commonly used in research, this container is referred to as Tissue Culture Flask.Tissue Culture Flask It is usually made of bottle body and bottle cap, wherein bottle cap includes sealing cover, ventilating cover and polyester lid etc. there are many type.Cell culture item Part needs the ventilating cover that generallys use of gas exchanges, and ventilating cover has air filtering function.Gas is passing through existing ventilating cover When, usually straight directly to go out, it is shorter that gas flows stroke, it is difficult to guarantee that gas filtration is thorough, influence cell culture fluid.
Utility model content
When carrying out gas exchanges to solve the Tissue Culture Flask mentioned in background technique, because gas flowing stroke is shorter, In the presence of filtering halfway problem, the utility model provides a kind of high efficiency cell culture bottle air filter and cell culture Bottle, wherein high efficiency cell culture bottle air filter, including lid and filter body;
Embedded hole is equipped at the top of the lid;The embedded hole is connected with concave station;The concave station is equipped with opening;The concave station Pass through the open communication with the inner cavity of the lid;
The filter body top, bottom end and two sides are successively arranged the first filter membrane, the second filter membrane, third filter membrane and the 4th filter Film;The filter core intracoelomic cavity is equipped with demarcation plate so that first filter membrane is connected to the third filter membrane, second filter membrane and The 4th filter membrane connection;
The filter body is on the concave station;The filter body top matches with the embedded hole;With described The end face of four filter membranes matches with the opening;It is respectively kept between the concave station and second filter membrane and the third filter membrane Gap.
Further, the filter body is cube shape.
Further, the end surface side with the 4th filter membrane is along equipped with notch;End face with the 4th filter membrane is logical It is chimeric with the edge of the opening to cross the notch.
Further, first filter membrane, second filter membrane, the third filter membrane, the 4th filter membrane are detachable It is connected on the end face of shown filter body.
Further, first filter membrane, second filter membrane, the third filter membrane and the 4th filter membrane are respectively set In on filter frame, the end face of the filter body is equipped with installation window, and the filter frame is on the installation window.
A kind of Tissue Culture Flask, using above-mentioned high efficiency cell culture bottle air filter;The lid spiral cover in Bottle mouth position.
High efficiency cell culture bottle air filter provided by the utility model is increased by the cooperation of filter body and concave station Gas flows stroke, so that air filtration is more thorough;In addition, filter body is equipped with level Four filter membrane, air passes through multistage filtering, only Change effect become apparent from, is more efficient.Tissue Culture Flask provided by the utility model, using above-mentioned high efficiency cell culture bottle air filtration Device has efficient air filtration effect as bottle cap, and leads to cell culture fluid once maloperation occurs in experimentation Leaching touching bottle cap, since the preceding three-level filter membrane of the bottle cap almost filters out most impurity of air, the 4th filter membrane itself is not With any gas filtration impurity, avoids cell culture fluid and polluted by bottle cap.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is Some embodiments of the utility model, for those of ordinary skill in the art, in the premise of not making the creative labor property Under, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is the schematic cross-section of Tissue Culture Flask air filter provided by the utility model;
Fig. 2 is the semi-cutaway of lid;
Fig. 3 is the structural schematic diagram of filter body;
Fig. 4 is the structural schematic diagram of filter frame;
Fig. 5 is the structural schematic diagram of Tissue Culture Flask.
Appended drawing reference:
10 lid, 11 embedded hole, 12 concave station
13 opening 20 filter body, 21 first filter membranes
The 4th filter membrane of 22 second filter membrane, 23 third filter membrane 24
25 demarcation plate, 26 notch, 27 filter frame
28 installation 30 bottle bodies of window
Specific embodiment
It is practical new below in conjunction with this to keep the objectives, technical solutions, and advantages of the embodiments of the present invention clearer Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched The embodiment stated is the utility model a part of the embodiment, instead of all the embodiments.Based on the implementation in the utility model Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to The range of the utility model protection.
In the description of the present invention, it should be noted that term " center ", " longitudinal direction ", " transverse direction ", "upper", "lower", The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of describing the present invention and simplifying the description, rather than indicate Or imply that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore cannot understand For limitations of the present invention.In addition, term " first ", " second " are used for description purposes only, and should not be understood as instruction or Imply relative importance.
A kind of high efficiency cell culture bottle air filter, including lid 10 and filter body 20;
Embedded hole 11 is equipped at the top of the lid 10;The embedded hole 11 is connected with concave station 12;The concave station 12 is equipped with and opens Mouth 13;The concave station 12 is connected to the inner cavity of the lid 10 by the opening 13;
20 top of filter body, bottom end and two sides are successively arranged the first filter membrane 21, the second filter membrane 22,23 and of third filter membrane 4th filter membrane 24;20 inner cavity of filter body is equipped with demarcation plate 25, so that first filter membrane 21 and the third filter membrane 23 connect Logical, second filter membrane 22 is connected to the 4th filter membrane 24;
The filter body 20 is on the concave station 12;20 top of filter body matches with the embedded hole 11;Band There is the end face of the 4th filter membrane 24 to match with the opening 13;The concave station 12 and second filter membrane 22 and the third Gap is respectively kept between filter membrane 23.
Preferably, the filter body 20 is cube shape.
When it is implemented, as shown in Figure 1-Figure 3, the bottom of lid 10 is lid mouth, the housing cross-sectional of lid 10 is in character cut in bas-relief Type;The centre of 10 top shell of lid is equipped with embedded hole 11, the top end of embedded hole 11 size and shape and filter body 20 Face size and shape are identical;The inner cavity of lid 10 is molded with and the integrally formed concave station 12 of shell;Preferably, the shape of concave station 12 It is identical as the shape of filter body 20;
More excellent filter body 20 is cube shape, for convenience it will be understood by those skilled in the art that the utility model, concave station 12 are excellent Cube shape is selected as to be described;The top edge of 12 3 sides of concave station is connect with 11 edge of embedded hole, and the another side of concave station 12 is set There is an opening 13;Concave station 12 is connected to by opening 13 with 10 inner cavity of lid;
20 top end face of filter body is equipped with the first filter membrane 21 being detachably connected;20 bottom face of filter body is equipped with and is detachably connected The second filter membrane 22;The opposite both sides of the face of filter body 20 are respectively equipped with third filter membrane 23 and the 4th filter membrane 24;First filter membrane 21 For 0.22 μm of the chlorinated polyvinyl chloride fibre filter membrane in aperture, there is hydrophobicity, for ventilating, can cross solid particle in air filtering and Most of bacterium;Second filter membrane 22 and third filter membrane 23 are the teflon membrane filter in 0.02 μm of aperture can for ventilating Most of virus in air filtering is effectively crossed, prevents cell culture fluid from exosmosing;4th filter membrane 24 is the polytetrafluoroethyl-ne in 0.22 μm of aperture There is alkene filter membrane hydrophobicity can prevent cell culture fluid from exosmosing for ventilating;20 boring of filter body, inner cavity is equipped with Demarcation plate 25, demarcation plate 25 separate the first filter membrane 21 and third filter membrane 23 with the second filter membrane 22 and the 4th filter membrane 24;First filter Film 21 is connected to third filter membrane 23 by the cavity of 25 side of demarcation plate, and the second filter membrane 22 and the 4th filter membrane 24 pass through demarcation plate 25 The cavity of the other side is connected to;
For filter body 20 through snap fitting structures such as buckle or card slots on concave station 12, filter body 20 and concave station 12 are detachable Connection;The edge of 20 top end surface of filter body matches with the edge of embedded hole 11, good airproof performance, 20 top end surface of filter body Edge turns up, and the edge of embedded hole 11 is equipped with corresponding chamfering, improves the leakproofness of filter body top end face and embedded hole 11;Band The edge of the end face and opening 13 that have the 4th filter membrane 24 coincide, and the two concordantly connects, good airproof performance;The size of concave station 12 is greater than filter The size of core 20, so that there are gaps between concave station 12 and the second filter membrane 22 and third filter membrane 23;In use, gas passes through First filter membrane 21 flows into the inner cavity of filter body 20, flows out to concave station 12 from the second filter membrane 22, flows into filter from third filter membrane 23 The other side inner cavity of core 20, the lid mouth of lid 10 is flowed out to from the 4th filter membrane 24;The flowing stroke of whole process, gas is compared It is longer in ventilating cover, and pass through multistage filtering, filter effect is more significant.In addition, filter body 20, the first filter membrane 21, the second filter membrane 22, third filter membrane 23 and the 4th filter membrane 24 are all detachably connected, and are conveniently replaceable and are cleaned.
Preferably, the end surface side with the 4th filter membrane 24 is along equipped with notch 26;End with the 4th filter membrane 24 Face is chimeric with the edge of the opening 13 by the notch 26.For improving filter body 20 and 12 connection sealing of concave station.
When it is implemented, as shown in figures 1 and 3, end bottom edge and two sides edge with the 4th filter membrane 24 are all provided with There is notch 26, the width of notch 26 matches with the edge width of opening 13;13 edges be open in notch 26, filtering core 20 are fixedly connected on concave station 12 by the edge of opening 13 and the inlay card of notch 26, and carry out encapsulation process.
Preferably, first filter membrane 21, second filter membrane 22, the third filter membrane 23, the 4th filter membrane 24 are equal It is removably connected on the end face of shown filter body 20.
Preferably, first filter membrane 21, second filter membrane 22, the third filter membrane 23 and the 4th filter membrane 24 divide It is not set on filter frame 27, the end face of the filter body 20 is equipped with installation window 28, and the filter frame 27 is embedded in the installation window On 28.So that the first filter membrane 21, the second filter membrane 22, third filter membrane 23 and the 4th filter membrane 24 are detachably connected with filter body 20, just In replacement filter membrane.
When it is implemented, as shown in Figure 3 and Figure 4, the first filter membrane 21, the second filter membrane 22, third filter membrane 23 and the 4th filter membrane 24 edge is individually fixed on four filter frames 27.Four end faces that filter body 20 is used to install filter membrane are respectively equipped with installation window 28, filter frame 27 is connected by the installation window 28 of the structures such as buckle or card slot and filter body.
A kind of Tissue Culture Flask, using above-mentioned high efficiency cell culture bottle air filter;20 spiral cover of lid In bottle mouth position.
When it is implemented, as shown in figure 5, a kind of Tissue Culture Flask, using above-mentioned high efficiency cell culture bottle air mistake Device is filtered, lid 10 is equipped with internal screw thread, and the bottleneck of bottle body 30 is equipped with external screw thread, and 10 spiral cover of lid is in bottle mouth position.
High efficiency cell culture bottle air filter provided by the utility model is increased by the cooperation of filter body and concave station Gas flows stroke, so that air filtration is more thorough;In addition, filter body is equipped with level Four filter membrane, air passes through multistage filtering, only Change effect become apparent from, is more efficient.Tissue Culture Flask provided by the utility model, using above-mentioned high efficiency cell culture bottle air filtration Device has efficient air filtration effect as bottle cap, and leads to cell culture fluid once maloperation occurs in experimentation Leaching touching bottle cap, since the preceding three-level filter membrane of the bottle cap almost filters out most impurity of air, the 4th filter membrane itself is not With any gas filtration impurity, avoids cell culture fluid and polluted by bottle cap.
Although more herein used such as lid, embedded hole, concave station, opening, filter body, the first filter membrane, second Filter membrane, third filter membrane, the 4th filter membrane, demarcation plate, notch, filter frame, the installation terms such as window and bottle body, but be not precluded and use it A possibility that its term.The use of these terms is merely for the convenience of describing and explaining the nature of the invention;It To be construed to any additional limitation all be contrary to the spirit of the present invention.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited System;Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should Understand: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of Technical characteristic is equivalently replaced;And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical new The range of each embodiment technical solution of type.

Claims (6)

1. a kind of high efficiency cell culture bottle air filter, it is characterised in that: including lid (10) and filter body (20);
Embedded hole (11) are equipped at the top of the lid (10);The embedded hole (11) is connected with concave station (12);The concave station (12) Equipped with opening (13);The concave station (12) is connected to the inner cavity of the lid (10) by the opening (13);
Filter body (20) top, bottom end and two sides are successively arranged the first filter membrane (21), the second filter membrane (22), third filter membrane (23) and the 4th filter membrane (24);Filter body (20) inner cavity is equipped with demarcation plate (25), so that first filter membrane (21) and institute Third filter membrane (23) connection is stated, second filter membrane (22) is connected to the 4th filter membrane (24);
The filter body (20) is on the concave station (12);Filter body (20) top and the embedded hole (11) kissing It closes;End face with the 4th filter membrane (24) matches with the opening (13);The concave station (12) and second filter membrane (22) gap is respectively kept between the third filter membrane (23).
2. high efficiency cell culture bottle air filter according to claim 1, it is characterised in that: the filter body (20) For cube shape.
3. high efficiency cell culture bottle air filter according to claim 1, it is characterised in that: with the 4th filter The end surface side of film (24) is along equipped with notch (26);End face with the 4th filter membrane (24) by the notch (26) with it is described The edge of opening (13) is chimeric.
4. high efficiency cell culture bottle air filter according to claim 1, it is characterised in that: first filter membrane (21), second filter membrane (22), the third filter membrane (23), the 4th filter membrane (24) are removably connected to shown filter core On the end face of body (20).
5. high efficiency cell culture bottle air filter according to claim 4, it is characterised in that: first filter membrane (21), second filter membrane (22), the third filter membrane (23) and the 4th filter membrane (24) are respectively arranged on filter frame (27), The end face of the filter body (20) is equipped with installation window (28), and the filter frame (27) is on the installation window (28).
6. a kind of Tissue Culture Flask, it is characterised in that: use the described in any item high efficiency cell culture bottles of Claims 1 to 5 Air filter;Lid (10) spiral cover is in bottle mouth position.
CN201820972964.6U 2018-06-24 2018-06-24 A kind of high efficiency cell culture bottle air filter and Tissue Culture Flask Active CN208694541U (en)

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Application Number Priority Date Filing Date Title
CN201820972964.6U CN208694541U (en) 2018-06-24 2018-06-24 A kind of high efficiency cell culture bottle air filter and Tissue Culture Flask

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Application Number Priority Date Filing Date Title
CN201820972964.6U CN208694541U (en) 2018-06-24 2018-06-24 A kind of high efficiency cell culture bottle air filter and Tissue Culture Flask

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CN208694541U true CN208694541U (en) 2019-04-05

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109943485A (en) * 2019-04-11 2019-06-28 广州赛泊泰生物技术有限公司 Culture bacterium/cell shaking flask of active swap air

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109943485A (en) * 2019-04-11 2019-06-28 广州赛泊泰生物技术有限公司 Culture bacterium/cell shaking flask of active swap air
CN109943485B (en) * 2019-04-11 2023-11-21 江苏谱新生物医药有限公司 Shaking bottle for actively exchanging air for culturing bacteria/cells

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