CN113881547B - Anti-pollution scribing culture box - Google Patents

Anti-pollution scribing culture box Download PDF

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Publication number
CN113881547B
CN113881547B CN202111213268.XA CN202111213268A CN113881547B CN 113881547 B CN113881547 B CN 113881547B CN 202111213268 A CN202111213268 A CN 202111213268A CN 113881547 B CN113881547 B CN 113881547B
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culture
tank
shell
window
protective shell
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CN202111213268.XA
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CN113881547A (en
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肖军
刘伟国
熊军
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Well Care Wuhan Medical Technology Co ltd
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Well Care Wuhan Medical Technology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention belongs to the field of structural design of microorganism culture devices, and particularly relates to an anti-pollution streak culture box. Comprises a culture dish, a box cover and a movable cover plate; the middle part of the culture dish is provided with a culture tank, the rear side of the culture tank is provided with a retaining tank, and the bottom of the retaining tank is provided with a fixed adsorbent; a protective shell matched with the shape of the culture tank is arranged in the middle of the lower side of the box cover; the protective shell is tightly buckled on the culture tank; the left side and the right side of the front of the protective shell or the culture tank are provided with shell walls, the shell walls on the left side and the right side extend forwards and enclose a window, and the movable cover plate is arranged in the window in an openable manner; the anti-pollution streak culture box has the advantages of compact and simple structure, convenient use, convenient operation of the traditional culture dish, self-sealing structure, special inoculation, streak and sampling channels arranged in the culture box, convenient use and good sealing property.

Description

Anti-pollution scribing culture box
Technical Field
The invention belongs to the field of structural design of microorganism culture devices, and particularly relates to an anti-pollution streak culture box.
Background
The culture dish is an essential basic experimental apparatus in the fields of medical test, biological cultivation and the like, and is mainly used for forming a relatively stable and proper cultivation environment, isolating an internal environment and an external environment, preventing external interference and avoiding internal culture leakage. In the process of culturing, various bacteria and the like need to be inoculated onto substances such as culture mediums and the like in a culture dish by using tools such as an inoculating loop/an inoculating needle and the like, and are limited by the structure and the operation mode of the traditional culture dish, in the process of inoculating, the culture dish is often required to be opened to cause that the internal culture medium, biological materials and the like are exposed in an external space, when the external environment is not sterile, the problem that the biological materials or the culture mediums are polluted easily occurs, the problem also exists in the process of scribing, and the surface of the inoculating needle or the inoculating loop is adhered with the culture mediums and the biological materials after scribing, and the culture mediums and the biological materials need to be cleaned or recovered again, so that the conventional inoculating culture working procedure is complicated, the operation environment and the operation requirement are relatively high, and the efficiency is low.
Disclosure of Invention
The invention aims to provide the anti-pollution streaking culture box which has the advantages of compact and reliable structure, good sealing performance and strong anti-pollution capability, is beneficial to improving the sealing performance of inoculation and streaking operation, reducing the operation content and improving the operation efficiency.
In order to achieve the above purpose, the present invention adopts the following technical scheme.
An anti-pollution streaking culture box comprises a culture dish 1, a box cover 2 and a movable cover plate 4;
the culture dish 1 is a shell with an open upper side; a culture tank 10 is arranged in the middle of the culture dish 1, a guide part 1c is arranged at the front side of the culture tank 10, a retaining tank 11 is arranged at the rear side of the culture tank 10, and a fixed adsorbent 12 is arranged at the bottom of the retaining tank 11;
the box cover 2 is buckled on the culture dish 1, and the box cover 2 is a shell with an open lower side; the middle part of the lower side of the box cover 2 is provided with a protective shell 20 which is matched with the shape of the culture tank 10;
the protective shell 20 is tightly buckled on the culture tank 10, an inlet 1a is arranged at the front side of the wall of the protective shell 20 or the culture tank 10, and an outlet 1b is arranged at the rear side of the wall of the protective shell 20 or the culture tank 10;
the shell wall 21 is arranged on the left and right sides of the front of the protective shell 20 or the culture tank 10, the shell wall 21 on the left and right sides extends forwards and surrounds the window 2b, and the movable cover plate 4 is arranged in the window 2b in an openable and closable manner;
the inlet 1a is opposed to the guide portion 1c and the window 2b, and the outlet 1b is opposed to the culture vessel 10.
A further improvement or embodiment of the aforementioned contamination-preventive streaking cassette, wherein the inlet port 1a is provided in the front side wall of the culture tank 10; the guide part 1c is arranged at the front side of the introduction port 1a, and a guide plane 1e is arranged at the top of the guide part 1 c; the outlet 1b is provided in the rear side wall of the culture vessel 10, and the outlet 1b and the indwelling vessel 11 are connected by an inclined guide surface 1 d.
In a further improvement or specific embodiment of the aforementioned anti-contamination streaking culture cassette, a front bayonet 2a for abutting against the inlet 1a is provided on the front side of the protective housing 20, and a rear bayonet 2c for abutting against the outlet 1b is provided on the rear side of the protective housing 20.
A further improvement or specific embodiment of the aforementioned anti-contamination streaking culture cassette, wherein the housing wall 21 is provided on the protective housing 20, and the lower side of the housing wall 21 abuts against the upper side of the guide portion 1 c;
the left and right case walls 21 form an introduction passage, the front side of which communicates with the window 2b, and the rear side of which communicates with the introduction port 1 a.
A further improvement or embodiment of the aforementioned anti-contamination streaking cartridge, further comprising a magnetic streaking head 3, movably arranged in the culture dish;
the magnetic scribing head 3 comprises: a support cylinder 30 which can pass through the window 2b, the inlet 1a and the outlet 1b; a sliding scribing head 31 which is sleeved at the lower end of the supporting cylinder 30 and can slide up and down; a movable adsorbent 32 embedded in the upper end of the support cylinder 30; a compression spring 33 with both ends abutting against the movable absorber 32 and the sliding scribing head 31; the movable adsorbent 32 and the fixed adsorbent 12 are magnetically attracted to each other.
In a further improvement or a specific embodiment of the aforementioned anti-pollution streaking culture box, the opening at the lower end of the support cylinder 30 is provided with a step hole 30a, and the upper end of the sliding streaking head 31 is larger than the hole diameter of the step hole 30a so as to be blocked on the step of the limit step hole 30 a;
the upper end of the sliding scribing head 31 is provided with a pressing groove 31a which can accommodate the lower end of the compression spring 33, and the lower end of the sliding scribing head 31 is provided with an inoculating loop 31b.
In a further improvement or a specific embodiment of the anti-pollution streaking culture box, the upper end and the lower end of the movable cover plate 4 are provided with short shafts, and the movable cover plate 4 is rotatably connected with the upper side and the lower side of the window 2b through the short shafts at the two ends; a torsion spring is arranged between the movable cover plate 4 and the box cover 2 so as to cover the window 2b; the movable cover plate 4 is provided with a transversely extending extrusion strip 3a on one side facing the culture tank 10, and an arc-shaped contact tank 3b is arranged in the middle of the extrusion strip 3 a.
In a further improvement or embodiment of the aforementioned anti-contamination streaking culture cassette, the bottom of the culture dish 1 is provided with a plurality of positioning posts 15 and positioning slots 16.
In a further improvement or a specific embodiment of the anti-pollution streaking culture box, the bottom of the culture dish 1 is provided with an adsorbent mounting groove 17, the adsorbent mounting groove 17 is opposite to the retaining groove 11, and the fixed adsorbent 12 is embedded in the adsorbent mounting groove 17.
The beneficial effects are that:
the anti-pollution streak culture box has the advantages of compact and simple structure, convenient use, convenient operation of the traditional culture dish, self-sealing structure, special inoculation, streaking and sampling channels arranged in the culture box, non-contact inoculation streaking operation realized by means of the magnetic adsorption device matched with the sampling piece, storage structure attached in the culture box, storage of the sampling piece after use, no need of reprocessing, convenient use and good sealing property.
Drawings
FIG. 1 is a schematic view of a structure of an anti-contamination streaking incubator;
FIG. 2 is a schematic diagram of a second embodiment of an anti-contamination streaking incubator;
FIG. 3 is a schematic diagram III of the structure of an anti-contamination streaking incubator;
FIG. 4 is an assembly view of the anti-contamination streaking cassette;
FIG. 5 is a schematic structural view of a magnetic line marking head;
wherein the reference numerals include:
culture dish 1, inlet 1a, outlet 1b, guide 1c, inclined guide surface 1d, guide plane 1e, culture tank 10, retention tank 11, fixed adsorbent 12, positioning column 15, positioning tank 16, adsorbent mounting groove 17, lid 2, front bayonet 2a, window 2b, rear bayonet 2c, protective case 20, case wall 21, magnetic scribing head 3, support cylinder 30, limit stepped hole 30a, sliding scribing head 31, pressing tank 31a, inoculating loop 31b, movable adsorbent 32, compression spring 33, movable cover plate 4.
Detailed Description
The present invention will be described in detail with reference to specific examples.
The anti-pollution scribing culture box is mainly used as a culture box part in a closed scribing culture device, has the characteristics of high sealing performance, convenient use, simple structure and low production cost, and can realize scribing operation without opening the culture box by being matched with an internal magnetic scribing head.
The basic structure is shown in figures 1, 2, 3 and 4, and comprises a culture dish 1, a box cover 2 and a movable cover plate 4;
wherein the culture dish 1 is a shell with an open upper side; the middle part of the culture dish 1 is provided with a culture tank 10, wherein the front side of the culture tank 10 is provided with a guide part 1c, the rear side of the culture tank 10 is provided with a retaining tank 11, and the bottom of the retaining tank 11 is provided with a fixed adsorbent 12;
wherein the box cover 2 is buckled on the culture dish 1, and the box cover 2 is a shell with an open lower side; the middle part of the lower side of the box cover 2 is provided with a protective shell 20 which is matched with the shape of the culture tank 10;
wherein the protective shell 20 is tightly buckled on the culture tank 10, the front side of the wall of the protective shell 20 or the culture tank 10 is provided with an inlet 1a, and the rear side of the wall of the protective shell 20 or the culture tank 10 is provided with an outlet 1b;
wherein the shell wall 21 is arranged on the left and right sides of the front of the protective shell 20 or the culture tank 10, the shell wall 21 on the left and right sides extends forwards and encloses a window 2b, and the movable cover plate 4 is arranged in the window 2b in an openable and closable manner;
wherein the introduction port 1a is opposed to the guide portion 1c and the window 2b, and wherein the introduction port 1b is opposed to the culture vessel 10.
Wherein the introduction port 1a is located at the front side tank wall of the culture tank 10; the guide part 1c is arranged at the front side of the introduction port 1a, and a guide plane 1e is arranged at the top of the guide part 1 c; wherein the outlet port 1b is provided in the rear side wall of the culture vessel 10, and wherein the outlet port 1b is connected to the indwelling vessel 11 by an inclined guide surface 1 d.
In this embodiment, since the culture dish needs to be separately operated before and after the use and preparation process so as to be convenient for pouring the culture medium, etc., the lid portion does not need to be operated, but only assembled at the end, in order to simplify the operation, in this embodiment, a complex structure is designed on the lid 2, specifically:
a front bayonet 2a for abutting against the inlet 1a is provided on the front side of the protective case 20, and a rear bayonet 2c for abutting against the outlet 1b is provided on the rear side of the protective case 20.
The front buckle, the leading-in port, the leading-out port and the rear buckle are utilized to respectively form a moving passage port of the scribing head, so that the scribing head can be moved and positioned conveniently.
As a more concise structural design scheme, the shell wall 21 is arranged on the protective shell 20, wherein the lower side of the shell wall 21 is propped against the upper side of the guide part 1 c; the left and right case walls 21 form an introduction passage, the front side of which communicates with the window 2b, and the rear side of which communicates with the introduction port 1 a.
In particular, in order to facilitate the scribing operation, the present application further provides a magnetic scribing head 3 capable of performing an actual scribing operation in cooperation with the above-mentioned culture box, which adopts a movable arrangement manner, and can utilize an external magnetic adsorption device to drive the magnetic scribing head to act so as to realize the transfer and scribing operation of microorganisms or reagents; specifically:
as shown in fig. 4, wherein the magnetic scribing head 3 comprises: a support cylinder 30 which can pass through the window 2b, the inlet 1a and the outlet 1b; a sliding scribing head 31 which is sleeved at the lower end of the supporting cylinder 30 and can slide up and down; a movable adsorbent 32 embedded in the upper end of the support cylinder 30; a compression spring 33 with both ends abutting against the movable absorber 32 and the sliding scribing head 31; wherein the movable adsorbent 32 and the fixed adsorbent 12 are magnetically attracted to each other.
In particular, to simplify the installation and use of the magnetic scribing head,
wherein the lower end opening of the supporting cylinder 30 is provided with a step hole 30a, and the upper end of the sliding scribing head 31 is larger than the aperture of the step hole 30a so as to be clamped on the step of the limiting step hole 30 a;
wherein the upper end of the sliding scribing head 31 is provided with a pressing groove 31a which can accommodate the lower end of the compression spring 33, and the lower end of the sliding scribing head 31 is provided with an inoculating loop 31b.
Wherein the upper and lower ends of the movable cover plate 4 are provided with short shafts, and the movable cover plate 4 is rotatably connected with the upper and lower sides of the window 2b through the short shafts at the two ends; wherein, one side of the movable cover plate 4 facing the culture tank 10 is provided with a squeezing strip 3a extending transversely, and the middle part of the squeezing strip 3a is provided with an arc-shaped contact tank 3b.
Further, the device is mainly used in combination with an automatic culture marking device, and in order to ensure the precision of marking and transferring operations, the culture box needs to be fixed in relative position on the device, and for this purpose, in this embodiment, a plurality of positioning columns 15 and positioning slots 16 are provided at the bottom of the culture dish 1. The culture box is fixed by the positioning column and the positioning groove, so that the relative position of the culture box is kept fixed.
In order to facilitate the installation of the fixed adsorbent, an adsorbent installation groove 17 is arranged at the bottom of the box of the culture dish 1, wherein the adsorbent installation groove 17 is opposite to the retaining groove 11, and the fixed adsorbent 12 is embedded in the adsorbent installation groove 17.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention.

Claims (5)

1. An anti-pollution streaking culture box is characterized by comprising a culture dish (1), a box cover (2) and a movable cover plate (4);
the culture dish (1) is a shell with an open upper side; the middle part of the culture dish (1) is provided with a culture tank (10), the front side of the culture tank (10) is provided with a guide part (1 c), the rear side of the culture tank (10) is provided with a retaining tank (11), and the bottom of the retaining tank (11) is provided with a fixed adsorbent (12);
the box cover (2) is buckled on the culture dish (1), and the box cover (2) is a shell with an open lower side; a protective shell (20) matched with the shape of the culture tank (10) is arranged in the middle of the lower side of the box cover (2);
the protective shell (20) is tightly buckled on the culture tank (10), an introduction port (1 a) is arranged at the front side of the protective shell (20) or the tank wall of the culture tank (10), and an outlet port (1 b) is arranged at the rear side of the protective shell (20) or the tank wall of the culture tank (10);
the left side and the right side of the front of the protective shell (20) or the culture tank (10) are provided with shell walls (21), the shell walls (21) on the left side and the right side extend forwards and enclose a window (2 b), and the movable cover plate (4) is arranged in the window (2 b) in an openable manner;
the leading-in port (1 a) is opposite to the guide part (1 c) and the window (2 b), and the leading-out port (1 b) is opposite to the culture tank (10);
the introducing port (1 a) is positioned at the front side groove wall of the culture groove (10); the guide part (1 c) is arranged at the front side of the introduction port (1 a), and a guide plane (1 e) is arranged at the top of the guide part (1 c); the outlet (1 b) is arranged on the rear side groove wall of the culture groove (10), and the outlet (1 b) is connected with the retaining groove (11) through an inclined guide surface (1 d);
the shell wall (21) is arranged on the protective shell (20), and the lower side of the shell wall (21) is propped against the upper side of the guide part (1 c); the left and right shell walls (21) form an introduction channel, the front side of the introduction channel is communicated with the window (2 b), and the rear side of the introduction channel is communicated with the introduction port (1 a);
the culture dish (1) further comprises a magnetic scribing head (3) movably arranged in the culture dish (1); the magnetic scribing head (3) comprises: a support cylinder (30) which can pass through the window (2 b), the inlet (1 a) and the outlet (1 b); a sliding scribing head (31) sleeved at the lower end of the supporting cylinder (30) and capable of sliding up and down; a movable adsorption body (32) embedded at the upper end of the supporting cylinder (30); a compression spring (33) with two ends abutting against the movable adsorbing body (32) and the sliding scribing head (31); the movable adsorbent (32) and the fixed adsorbent (12) can be mutually adsorbed by magnetism;
the lower end opening of the supporting cylinder (30) is provided with a step hole (30 a), and the upper end of the sliding scribing head (31) is larger than the aperture of the step hole (30 a) so as to be clamped on the step of the limit step hole (30 a);
the upper end of the sliding scribing head (31) is provided with an extrusion groove (31 a) which can accommodate the lower end of the compression spring (33), and the lower end of the sliding scribing head (31) is provided with an inoculating loop (31 b).
2. The anti-pollution streaking culture box according to claim 1, wherein a front bayonet (2 a) which is in butt joint with the inlet (1 a) is arranged on the front side of the protective shell (20), and a rear bayonet (2 c) which is in butt joint with the outlet (1 b) is arranged on the rear side of the protective shell (20).
3. The anti-pollution streaking culture box according to claim 1, wherein the upper and lower ends of the movable cover plate (4) are provided with short shafts, and the movable cover plate (4) is rotatably connected to the upper and lower sides of the window (2 b) through the short shafts at the two ends; a torsion spring is arranged between the movable cover plate (4) and the box cover (2) so as to cover the window (2 b);
one side of the movable cover plate (4) facing the culture tank (10) is provided with a transversely extending extrusion strip (3 a), and the middle part of the extrusion strip (3 a) is provided with an arc-shaped contact groove (3 b).
4. An anti-contamination streaking culture cassette as claimed in claim 1, wherein the cassette bottom of the culture dish (1) is provided with a plurality of positioning posts (15) and positioning slots (16).
5. The anti-pollution streaking culture box according to claim 1, wherein an adsorbent mounting groove (17) is formed in the bottom of the culture dish (1), the adsorbent mounting groove (17) is opposite to the retaining groove (11), and the fixed adsorbent (12) is embedded in the adsorbent mounting groove (17).
CN202111213268.XA 2021-10-19 2021-10-19 Anti-pollution scribing culture box Active CN113881547B (en)

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Application Number Priority Date Filing Date Title
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CN113881547B true CN113881547B (en) 2024-03-26

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Publication number Priority date Publication date Assignee Title
CN114525199A (en) * 2022-01-19 2022-05-24 武汉旭德喜生物科技有限公司 Automatic marking-off culture apparatus culture dish locating component

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105695316A (en) * 2016-04-13 2016-06-22 中国科学院苏州生物医学工程技术研究所 Microorganism scratching device
CN209619349U (en) * 2019-01-18 2019-11-12 深圳市优圣康生物科技有限公司 A kind of antipollution culture dish
CN210261744U (en) * 2019-06-27 2020-04-07 魏小玲 Microbial cultivation box
CN210945585U (en) * 2019-06-11 2020-07-07 福建万邦清源环保科技有限公司 Anti-pollution culture dish convenient to operate

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105695316A (en) * 2016-04-13 2016-06-22 中国科学院苏州生物医学工程技术研究所 Microorganism scratching device
CN209619349U (en) * 2019-01-18 2019-11-12 深圳市优圣康生物科技有限公司 A kind of antipollution culture dish
CN210945585U (en) * 2019-06-11 2020-07-07 福建万邦清源环保科技有限公司 Anti-pollution culture dish convenient to operate
CN210261744U (en) * 2019-06-27 2020-04-07 魏小玲 Microbial cultivation box

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