CN105695316A - Microorganism scratching device - Google Patents
Microorganism scratching device Download PDFInfo
- Publication number
- CN105695316A CN105695316A CN201610226902.6A CN201610226902A CN105695316A CN 105695316 A CN105695316 A CN 105695316A CN 201610226902 A CN201610226902 A CN 201610226902A CN 105695316 A CN105695316 A CN 105695316A
- Authority
- CN
- China
- Prior art keywords
- culture dish
- upper cover
- twisted steel
- groove
- face
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
Abstract
The invention discloses a microorganism scratching device. The microorganism scratching device comprises a culture dish, an upper cover and a coating brush, wherein the culture dish is in the shape of a disk with an upward opening; a culture medium covers a bottom end surface of the inside of the culture dish; a liquid storage groove is formed in the bottom end surface of the inside of the culture dish; the upper cover is matched with the culture dish and is in the shape of a disk with a downward opening; a sample adding opening which corresponds to the liquid storage groove is formed in an end surface of the upper cover; the top of the coating brush is connected to the end surface of the upper cover; and when the upper cover covers the culture dish, the bottom of the coating brush is attached to the bottom end surface of the inside of the culture dish. The microorganism scratching device is safe and reliable, and convenient and fast to operate, and the upper cover does not need to be opened when microorganisms are scratched.
Description
Technical field
The present invention relates to the separation and Culture of microorganism and identification technology field, be specifically related to a kind of microbial streaking device。
Background technology
At present, in the field of Isolation and identification of microorganism, it mostly is multiple different strain or the mixture of different subtype microorganism due to detected sample, to carry out sample identifying accurately or subsequent experimental, needing to obtain purer microbe colony, therefore, microorganism list bacterium colony separates most important。
In actual mechanical process, the separation of bacterium colony is generally by technical staff's manual operations: aseptically, technical staff opens the upper cover of culture dish, the line in the first direction on culture medium flat plate surface with metal or plastics inoculating loop, formed and the first cut band produces multiple cut, then by inoculating loop sterilize after or use new ring stride across described first cut band different directions rule, produce the second cut band, same aforementioned operation, general also need to mark the 3rd even the 4th cut band, inoculation for substantial amounts of sample, substantial amounts of repetitive operation is easily caused by maloperation, to diagnosis, identify and bring the consequence that error is even serious。
Further, since need in operating process to open the upper cover of culture dish, the time that upper cover is lifted is more long, and the risk of cross-contamination is more big, and during for isolation identification pathogenic microorganism, such operation also adds operator virtually and infects the risk of relevant disease。
Summary of the invention
For the weak point existed in above-mentioned technology, the invention provides a kind of safe and reliable, simple operation, and without opening the microbial streaking device of upper cover when microorganism is carried out scoring operation。
The technical solution adopted for the present invention to solve the technical problems is: a kind of microbial streaking device, including: culture dish, it is the discoid of upward opening, and the inner bottom end face overlying of described culture dish is stamped culture medium, and offers a reservoir；Upper cover, it, in being mutually matched with described culture dish and downwardly open discoid, the end face of described upper cover offers an application of sample opening corresponding with described reservoir；Coating brush, its top is connected on the end face of described upper cover。
Preferably, described coating brush is fixing with described upper cover is connected or one-body molded。
Preferably, the upper side in described reservoir of inner bottom end face of described culture dish is provided with storing district, and when the relatively described culture dish of described upper cover is positioned at initial position and final position, described coating brush is respectively positioned in described storing district。
Preferably, the top, lateral wall of described culture dish is provided with limited block, and described limited block offers the first thread groove, is provided with and keeps out bar bottom the lateral wall of described culture dish, described in keep out bar offer the first groove being positioned at below described limited block；The medial wall of described upper cover is provided with the first twisted steel being mutually matched with described first thread groove, inside the end face of described upper cover, is equidistantly arranged at intervals with some projections。
Preferably, when the relatively described culture dish of described upper cover is positioned at initial position, the bottom of described first twisted steel is supported mutually with described bar of keeping out, and to cause the top end face of described projection and described culture dish to be, a determining deviation is arranged；When the relatively described culture dish of described upper cover is positioned at final position, the top of described first twisted steel is supported mutually with described limited block, and the bottom of described first twisted steel is positioned at the top of described first groove；When the relatively described culture dish of described upper cover is positioned at back-out position, the top of described first twisted steel is right against the screw terminal of described first thread groove, and the bottom of described first twisted steel is in described first groove, to cause the top end face setting bonded to each other of described projection and described culture dish。
Preferably, the top, lateral wall of described culture dish is additionally provided with locating piece, and described first twisted steel offers the breach matched with described locating piece；When the relatively described culture dish of described upper cover is positioned at initial position, described locating piece is just to described breach。
Preferably, described keep out article is additionally provided with second groove spaced apart with described first groove, the 3rd groove and the 4th groove, and the medial wall of described upper cover is additionally provided with second twisted steel spaced apart with described first twisted steel, the 3rd twisted steel and the 4th twisted steel；When the relatively described culture dish of described upper cover is positioned at final position, the bottom of described second twisted steel is positioned at the top of described second groove, the bottom of described 3rd twisted steel is positioned at the top of described 3rd groove, and the bottom of described 4th twisted steel is positioned at the top of described 4th groove。
The present invention is compared with prior art, it provides the benefit that: microbial streaking device provided by the invention, by being added from application of sample opening by sample the reservoir of culture dish, and rotary upper cover is to drive coating brush to rotate, so that the sample in reservoir is carried out automatic cut by coating brush；This microbial streaking device, safe and reliable, simple operation, and without opening upper cover when microorganism is carried out scoring operation。
Accompanying drawing explanation
Fig. 1 is the decomposing schematic representation of microbial streaking device of the present invention；
Fig. 2 is the structural representation of upper cover of the present invention；
Fig. 3 is the structural representation of culture dish of the present invention；
Fig. 4 is the structural representation of culture dish of the present invention；
Fig. 5 is the structural representation of coating brush of the present invention；
Fig. 6 is the structural representation that coating brush of the present invention is connected to when covering；
Fig. 7 is upper cover of the present invention relatively described culture dish schematic diagram when being positioned at initial position；
Fig. 8 is upper cover of the present invention relatively described culture dish schematic diagram when being positioned at final position；
Fig. 9 is the schematic diagram that the relatively described culture dish of upper cover of the present invention is positioned at when screwing out position；
In figure: 01 upper cover；011 application of sample opening；012 fixing hole；013 projection；014 first twisted steel；0141 breach；The top of 0142 first twisted steel；The bottom of 0143 first twisted steel；015 second twisted steel；016 the 3rd twisted steel；017 the 4th twisted steel；The end face of 018 upper cover；The medial wall of 019 upper cover；02 culture dish；021 puts district；022 reservoir；023 locating piece；024 first thread groove；025 limited block；The lateral wall of 026 culture dish；027 keeps out bar；0271 first groove；0272 second groove；0273 the 3rd groove；0274 the 4th groove；The inner bottom end face of 028 culture dish；The top end face of 029 culture dish；03 coating brush；The top of 031 coating brush；The bottom of 032 coating brush；033 card column。
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described in further detail, to make those skilled in the art can implement according to this with reference to description word。
As it is shown in figure 1, the invention provides a kind of microbial streaking device, including: culture dish 02, upper cover 01 and coating brush 03。
As shown in Fig. 34, it is the discoid of upward opening that described culture dish 02 is arranged, the inner bottom end face 028 of described culture dish is coated with culture medium, and offer a reservoir 022, described reservoir 022 is in fan shape or oblong-shaped, and the length of described reservoir 022 is less than the radius of described culture dish 02；The side being positioned at described reservoir 022 on the inner bottom end face 028 of described culture dish is provided with storing district 021, described storing district 021 is in fan shape, its corresponding central angle range for 5 °～180 °, when the relatively described culture dish 02 of described upper cover 01 is positioned at initial position and final position, described coating brush 03 is respectively positioned in described storing district 021；The top, lateral wall 026 of described culture dish is provided with limited block 025, described limited block 025 offers the first thread groove 024, it is provided with bottom the lateral wall 026 of described culture dish and keeps out bar 027, described keeping out offers the first groove 0271 being positioned at below described limited block 025 on bar 027, described in keep out and be additionally provided with second groove 0272, threeth groove 0273 and fourth groove 0274 spaced apart with described first groove 0271 on article 027；The top, lateral wall 026 of described culture dish is additionally provided with locating piece 023。
As in figure 2 it is shown, described upper cover 01 is arranged in being mutually matched with described culture dish 02 and downwardly open discoid, the end face 018 of described upper cover offers an application of sample opening 011 corresponding with described reservoir 022；The medial wall 019 of described upper cover is provided with the first twisted steel 014 being mutually matched with described first thread groove 024, described first twisted steel 014 offers the breach 0141 matched with described locating piece 023；Some projections 013 equidistantly it are arranged at intervals with inside the end face 018 of described upper cover；The medial wall 019 of described upper cover is additionally provided with second twisted steel 015, threeth twisted steel 016 and fourth twisted steel 017 spaced apart with described first twisted steel 014；Wherein, the central angle at described first corresponding described upper cover 01 center of twisted steel 014 is 27 °, described second twisted steel 015, the central angle at the 3rd twisted steel 016 and corresponding described upper cover 01 center of the 4th twisted steel 017 is 17 °, and corresponding central angle of being separated by between described first twisted steel 014 and the 4th twisted steel 017 is 38 °, corresponding central angle of being separated by between described first twisted steel 014 and the second twisted steel 015 is 73 °, corresponding central angle of being separated by between described second twisted steel 015 and the 3rd twisted steel 016 is 63 °, corresponding central angle of being separated by between described 3rd twisted steel 016 and the 4th twisted steel 017 is 108 °。
As shown in Fig. 56, the top 031 of described coating brush is connected on the end face 018 of described upper cover, described coating brush 03 is fixing with described upper cover 01 to be connected or one-body molded, the top 031 of described coating brush is provided with some card columns 033, and the end face 018 of described upper cover offers the fixing hole 012 that some and described card column 033 one_to_one corresponding is arranged；When described upper cover 01 cover cap is on described culture dish 02, the bottom 032 of described coating brush is affixed with the inner bottom end face 028 of described culture dish。
The work process of this microbial streaking device is as follows: by upper cover 01 cover cap on culture dish 02, and make the relatively described culture dish 02 of described upper cover 01 be positioned at initial position (as shown in Figure 7), now described locating piece 023 is just to described breach 0141, the bottom 0143 of described first twisted steel is supported with described bar 027 phase of keeping out, the top end face 029 of described projection 013 and described culture dish is separated by regular intervals, sample is added to the reservoir 022 of culture dish from application of sample opening 011, and rotary upper cover 01 is to drive coating brush 03 to rotate, so that the sample in reservoir 022 is carried out automatic cut by coating brush 03；When described upper cover 01 turns to when relatively described culture dish 02 is positioned at final position (as shown in Figure 8), the top 0142 of described first twisted steel is supported with described limited block 025 phase, the bottom 0143 of described first twisted steel is positioned at the top of described first groove 0271, the bottom of described second twisted steel 015 is positioned at the top of described second groove 0272, the bottom of described 3rd twisted steel 016 is positioned at the top of described 3rd groove 0273, the bottom of described 4th twisted steel 017 is positioned at the top of described 4th groove 0274, now coating brush 03 completes the scoring operation to sample, and described upper cover 01 cannot be rotated further；Upper cover 01 is pressed under the axial direction of culture dish 02, until described projection 013 is bonded to each other with the top end face 029 of described culture dish, now the bottom 0143 of described first twisted steel is in described first groove 0271, the bottom of described second twisted steel 015 is positioned at described second groove 0272, the bottom of described 3rd twisted steel 016 is positioned at described 3rd groove 0273, the bottom of described 4th twisted steel 017 is positioned at described 4th groove 0274, and the top 0142 of described first twisted steel is right against the screw terminal of described first thread groove 024, so that the relatively described culture dish 02 of described upper cover 01 is positioned at back-out position (as shown in Figure 9), it is rotated further upper cover 01, first twisted steel 014 screws out along the first thread groove 024, so that upper cover 01 departs from culture dish 02, complete whole operating process；This microbial streaking device, safe and reliable, simple operation, and without opening upper cover 01 when microorganism is carried out scoring operation。
Although embodiment of the present invention are disclosed as above, but listed utilization that it is not limited in description and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, it is easily achieved other amendment, therefore, under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited to specific details and shown here as the legend with description。
Claims (7)
1. a microbial streaking device, it is characterised in that including:
Culture dish, it is the discoid of upward opening, and the inner bottom end face overlying of described culture dish is stamped culture medium, and offers a reservoir；
Upper cover, it, in being mutually matched with described culture dish and downwardly open discoid, the end face of described upper cover offers an application of sample opening corresponding with described reservoir；
Coating brush, its top is connected on the end face of described upper cover。
2. microbial streaking device as claimed in claim 1, it is characterised in that described coating brush is fixing with described upper cover to be connected or one-body molded。
3. microbial streaking device as claimed in claim 1, it is characterized in that, the upper side in described reservoir of inner bottom end face of described culture dish is provided with storing district, and when the relatively described culture dish of described upper cover is positioned at initial position and final position, described coating brush is respectively positioned in described storing district。
4. microbial streaking device as claimed in claim 1, it is characterized in that, the top, lateral wall of described culture dish is provided with limited block, described limited block offers the first thread groove, be provided with bottom the lateral wall of described culture dish and keep out bar, described in keep out bar offer the first groove being positioned at below described limited block；The medial wall of described upper cover is provided with the first twisted steel being mutually matched with described first thread groove, inside the end face of described upper cover, is equidistantly arranged at intervals with some projections。
5. microbial streaking device as claimed in claim 4, it is characterized in that, when the relatively described culture dish of described upper cover is positioned at initial position, the bottom of described first twisted steel is supported mutually with described bar of keeping out, and to cause the top end face of described projection and described culture dish to be, a determining deviation is arranged；When the relatively described culture dish of described upper cover is positioned at final position, the top of described first twisted steel is supported mutually with described limited block, and the bottom of described first twisted steel is positioned at the top of described first groove；When the relatively described culture dish of described upper cover is positioned at back-out position, the top of described first twisted steel is right against the screw terminal of described first thread groove, and the bottom of described first twisted steel is in described first groove, to cause the top end face setting bonded to each other of described projection and described culture dish。
6. microbial streaking device as claimed in claim 5, it is characterised in that the top, lateral wall of described culture dish is additionally provided with locating piece, and described first twisted steel offers the breach matched with described locating piece；When the relatively described culture dish of described upper cover is positioned at initial position, described locating piece is just to described breach。
7. microbial streaking device as claimed in claim 6, it is characterized in that, described keep out article is additionally provided with second groove spaced apart with described first groove, the 3rd groove and the 4th groove, and the medial wall of described upper cover is additionally provided with second twisted steel spaced apart with described first twisted steel, the 3rd twisted steel and the 4th twisted steel；When the relatively described culture dish of described upper cover is positioned at final position, the bottom of described second twisted steel is positioned at the top of described second groove, the bottom of described 3rd twisted steel is positioned at the top of described 3rd groove, and the bottom of described 4th twisted steel is positioned at the top of described 4th groove。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610226902.6A CN105695316B (en) | 2016-04-13 | 2016-04-13 | Microbial streaking device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610226902.6A CN105695316B (en) | 2016-04-13 | 2016-04-13 | Microbial streaking device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105695316A true CN105695316A (en) | 2016-06-22 |
| CN105695316B CN105695316B (en) | 2018-04-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610226902.6A Active CN105695316B (en) | 2016-04-13 | 2016-04-13 | Microbial streaking device |
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| CN (1) | CN105695316B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628281A (en) * | 2019-02-14 | 2019-04-16 | 严清园 | A kind of dedicated scoring devices of microbial culture dish |
| CN113881547A (en) * | 2021-10-19 | 2022-01-04 | 微尔创（武汉）医疗科技有限公司 | Anti-pollution marking-out culture box |
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| CN1918280A (en) * | 2004-01-22 | 2007-02-21 | 默德威特科技有限公司 | Microbial streaking device |
| US20110097790A1 (en) * | 2009-10-26 | 2011-04-28 | Biomerieux, Inc | Lockable cell growth chamber with aintilocking feature |
| JP2011211990A (en) * | 2010-04-01 | 2011-10-27 | Dainippon Printing Co Ltd | Petri dish for culture medium preparation |
| CN102559476A (en) * | 2010-12-30 | 2012-07-11 | 苏州生物医学工程技术研究所 | Microorganism seperation culture device |
| CN203582845U (en) * | 2013-09-29 | 2014-05-07 | 中国人民解放军第三军医大学 | Microorganism isolated culture device |
| CN205576141U (en) * | 2016-04-13 | 2016-09-14 | 中国科学院苏州生物医学工程技术研究所 | Microorganism mar device |
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2016
- 2016-04-13 CN CN201610226902.6A patent/CN105695316B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1918280A (en) * | 2004-01-22 | 2007-02-21 | 默德威特科技有限公司 | Microbial streaking device |
| US20110097790A1 (en) * | 2009-10-26 | 2011-04-28 | Biomerieux, Inc | Lockable cell growth chamber with aintilocking feature |
| JP2011211990A (en) * | 2010-04-01 | 2011-10-27 | Dainippon Printing Co Ltd | Petri dish for culture medium preparation |
| CN102559476A (en) * | 2010-12-30 | 2012-07-11 | 苏州生物医学工程技术研究所 | Microorganism seperation culture device |
| CN203582845U (en) * | 2013-09-29 | 2014-05-07 | 中国人民解放军第三军医大学 | Microorganism isolated culture device |
| CN205576141U (en) * | 2016-04-13 | 2016-09-14 | 中国科学院苏州生物医学工程技术研究所 | Microorganism mar device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628281A (en) * | 2019-02-14 | 2019-04-16 | 严清园 | A kind of dedicated scoring devices of microbial culture dish |
| CN113881547A (en) * | 2021-10-19 | 2022-01-04 | 微尔创（武汉）医疗科技有限公司 | Anti-pollution marking-out culture box |
| CN113881547B (en) * | 2021-10-19 | 2024-03-26 | 微尔创（武汉）医疗科技有限公司 | Anti-pollution scribing culture box |
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| Publication number | Publication date |
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| CN105695316B (en) | 2018-04-17 |
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