CN105695316B - Microbial streaking device - Google Patents
Microbial streaking device Download PDFInfo
- Publication number
- CN105695316B CN105695316B CN201610226902.6A CN201610226902A CN105695316B CN 105695316 B CN105695316 B CN 105695316B CN 201610226902 A CN201610226902 A CN 201610226902A CN 105695316 B CN105695316 B CN 105695316B
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- Prior art keywords
- upper cover
- culture dish
- groove
- twisted steel
- face
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of microbial streaking device, including：Culture dish, it is discoid in upward opening, and the inner bottom end face overlying of the culture dish is stamped culture medium, and offers a reservoir；Upper cover, it is in be mutually matched with the culture dish and downwardly open discoid, and one is offered on the end face of the upper cover and is open with the corresponding sample-adding of the reservoir；Coating brush, its top are connected on the end face of the upper cover；Wherein, when the upper cover the cover is when on the culture dish, the bottom of the coating brush and the inner bottom end face of the culture dish are affixed.The microbial streaking device, safe and reliable, simple operation, and to upper cover need not be opened during microorganism progress scoring operation.
Description
Technical field
The present invention relates to being separately cultured and identification technology field for microorganism, and in particular to a kind of microbial streaking device.
Background technology
At present, in the field of Isolation and identification of microorganism, since detected sample is mostly a variety of different strains or different Asias
The mixture of type microorganism, to sample carry out accurately identification or subsequent experimental, it is necessary to obtain purer microbial bacteria
Fall, therefore, the separation of microorganism single bacterium colony is most important.
In actual mechanical process, the separation of bacterium colony is usually by technical staff's manual operations：Aseptically, technology people
The upper cover of member's opening culture dish, with the line of metal or plastics oese in the first direction on culture medium flat plate surface, shape
Multiple cuts are produced on into the first cut band, are existed after then oese is sterilized or using new ring across the first cut band
Different directions are rule, and produce the second cut band, same to aforementioned operation, generally also needs to mark the 3rd or even the 4th cut band, for big
For the inoculation of the sample of amount, substantial amounts of repetitive operation easily causes maloperation, brings error even serious to diagnosing, identifying
Consequence.
Further, since needing to open the upper cover of culture dish in operating process, the time that upper cover is lifted is longer, cross contamination
Risk it is bigger, for separation identify pathogenic microorganism when, it is such operation also virtually add operating personnel infect phase
The risk of related disorders.
The content of the invention
For shortcoming present in above-mentioned technology, the present invention provides a kind of safe and reliable, simple operation, and to micro-
Biology need not be opened during scoring operation the microbial streaking device of upper cover.
The technical solution adopted by the present invention to solve the technical problems is：A kind of microbial streaking device, including：Culture
Ware, it is discoid in upward opening, and the inner bottom end face overlying of the culture dish is stamped culture medium, and offers a liquid storage
Groove；Upper cover, it is in be mutually matched with the culture dish and downwardly open discoid, offered on the end face of the upper cover one with
The corresponding sample-adding opening of reservoir；Coating brush, its top are connected on the end face of the upper cover.
Preferably, the coating brush is fixedly connected or is integrally formed with the upper cover.
Preferably, upper be provided with the side of the reservoir in the inner bottom end face of the culture dish puts area, works as institute
When stating the relatively described culture dish of upper cover and being located at initial position and final position, the coating brush is respectively positioned in the storing area.
Preferably, limited block is provided with the top of the lateral wall of the culture dish, the first screw thread is offered on the limited block
Groove, the lateral wall bottom of the culture dish, which is provided with, keeps out bar, and described keep out is offered on bar below the limited block
First groove；Be provided with the first twisted steel being mutually matched with first thread groove on the madial wall of the upper cover, it is described on
Some convex blocks are equidistantly arranged at intervals with the inside of the end face of lid.
Preferably, when the relatively described culture dish of the upper cover is located at initial position, the bottom of first twisted steel with
The bar of keeping out mutually is supported, to cause the top end face of the convex block and the culture dish to be set in a determining deviation；When the upper cover
When relatively described culture dish is located at final position, top and the limited block of first twisted steel are mutually supported, and described
The bottom of one twisted steel is located at the top of first groove；When the relatively described culture dish of the upper cover is positioned at back-out position,
The top of first twisted steel is right against the screw terminal of first thread groove, and the bottom of first twisted steel is in institute
State in the first groove, to cause the convex block and the top end face of the culture dish to be bonded to each other setting.
Preferably, be additionally provided with locating piece at the top of the lateral wall of the culture dish, offered on first twisted steel with
The notch that the locating piece matches；When the relatively described culture dish of the upper cover is located at initial position, the locating piece face
The notch.
Preferably, described keep out article is additionally provided with the second groove being spaced apart with first groove, the 3rd groove
With the 4th groove, the second twisted steel being spaced apart with first twisted steel, are additionally provided with the madial wall of the upper cover
Three twisted steels and the 4th twisted steel；When the relatively described culture dish of the upper cover is located at final position, second twisted steel
Bottom is located at the top of second groove, and the bottom of the 3rd twisted steel is located at the top of the 3rd groove, and described
The bottom of four twisted steels is located at the top of the 4th groove.
Compared with prior art, the present invention its advantage is：Microbial streaking device provided by the invention, by that will try
Sample is open from sample-adding and adds in the reservoir of culture dish, and rotary upper cover to be to drive coating brush to rotate, so that coating brush is to liquid storage
Sample in groove carries out automatic cut；The microbial streaking device, safe and reliable, simple operation, and cut is carried out to microorganism
Upper cover need not be opened during operation.
Brief description of the drawings
Fig. 1 is the decomposition diagram of microbial streaking device of the present invention；
Fig. 2 is the structure diagram of upper cover of the present invention；
Fig. 3 is the structure diagram of culture dish of the present invention；
Fig. 4 is the structure diagram of culture dish of the present invention；
Fig. 5 is the structure diagram of coating brush of the present invention；
Fig. 6 is that coating brush of the present invention is connected to structure diagram when covering；
Fig. 7 is schematic diagram when the relatively described culture dish of upper cover of the present invention is located at initial position；
Fig. 8 is schematic diagram when the relatively described culture dish of upper cover of the present invention is located at final position；
Fig. 9 is the relatively described culture dish of upper cover of the present invention positioned at schematic diagram when screwing out position；
In figure：01 upper cover；011 sample-adding opening；012 mounting hole；013 convex block；014 first twisted steel；0141 notch；0142
The top of first twisted steel；The bottom of 0143 first twisted steel；015 second twisted steel；016 the 3rd twisted steel；017 the 4th screw thread
Muscle；The end face of 018 upper cover；The madial wall of 019 upper cover；02 culture dish；021 puts area；022 reservoir；023 locating piece；024
One thread groove；025 limited block；The lateral wall of 026 culture dish；027 keeps out bar；0271 first groove；0272 second groove；0273
3rd groove；0274 the 4th groove；The inner bottom end face of 028 culture dish；The top end face of 029 culture dish；03 coating brush；031 applies
The top of cloth brush；The bottom of 032 coating brush；033 card column.
Embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art with reference to specification text
Word can be implemented according to this.
As shown in Figure 1, the present invention provides a kind of microbial streaking device, including：Culture dish 02, upper cover 01 and coating brush
03。
As shown in Fig. 3-4, the culture dish 02 sets discoid, the inner bottom of the culture dish in upward opening
Covered with culture medium on face 028, and a reservoir 022 is offered, the reservoir 022 is in fan shape or oblong-shaped, and institute
The length for stating reservoir 022 is less than the radius of the culture dish 02；It is located at the storage on the inner bottom end face 028 of the culture dish
The side of liquid bath 022 be provided with put area 021, it is described storing area 021 be in fan shape, its correspond to central angle scope be 5 °~
180 °, when the relatively described culture dish 02 of the upper cover 01 is located at initial position and final position, the coating brush 03 is respectively positioned on
In the storing area 021；The top of lateral wall 026 of the culture dish is provided with limited block 025, is opened up on the limited block 025
There is the first thread groove 024,026 bottom of lateral wall of the culture dish, which is provided with, keeps out bar 027, and described keep out opens up on bar 027
There is the first groove 0271 positioned at the lower section of limited block 025, described keep out is additionally provided with and first groove on bar 027
0271 the second groove 0272, the 3rd groove 0273 and the 4th groove 0274 being spaced apart；The lateral wall 026 of the culture dish pushes up
Portion is additionally provided with locating piece 023.
As shown in Fig. 2, the upper cover 01 is set in being mutually matched with the culture dish 02 and downwardly open discoid, institute
State and one and the corresponding sample-adding opening 011 of the reservoir 022 are offered on the end face 018 of upper cover；The madial wall of the upper cover
The first twisted steel 014 being mutually matched with first thread groove 024 is provided with 019, is opened up on first twisted steel 014
There is the notch 0141 to match with the locating piece 023；The inner side of end face 018 of the upper cover is equidistantly arranged at intervals with some convex
Block 013；The second twisted steel being spaced apart with first twisted steel 014 is additionally provided with the madial wall 019 of the upper cover
015th, the 3rd twisted steel 016 and the 4th twisted steel 017；Wherein, first twisted steel 014 corresponds to the circle at 01 center of upper cover
Heart angle is 27 °, and second twisted steel 015, the 3rd twisted steel 016 and the 4th twisted steel 017 correspond to 01 center of upper cover
Central angle is 17 °, and is separated by corresponding central angle between first twisted steel 014 and the 4th twisted steel 017 for 38 °, described
It is 73 ° to be separated by corresponding central angle between first twisted steel 014 and the second twisted steel 015, second twisted steel 015 and the 3rd
It is separated by corresponding central angle between twisted steel 016 for 63 °, is separated by between the 3rd twisted steel 016 and the 4th twisted steel 017 pair
The central angle answered is 108 °.
As shown in Fig. 5-6, the top 031 of the coating brush is connected on the end face 018 of the upper cover, the coating brush
03 is fixedly connected or is integrally formed with the upper cover 01, and the top 031 of the coating brush is provided with some card columns 033, it is described on
Some mounting holes 012 that setting is corresponded with the card column 033 are offered on the end face 018 of lid；When 01 the cover of upper cover
When on the culture dish 02, the bottom 032 of the coating brush is affixed with the inner bottom end face 028 of the culture dish.
The course of work of the microbial streaking device is as follows：By 01 the cover of upper cover on culture dish 02, and cause on described
The relatively described culture dish 02 of lid 01 is located at initial position (as shown in Figure 7), at this time notch described in 023 face of locating piece
0141, the bottom 0143 of first twisted steel is supported with 027 phase of bar of keeping out, the convex block 013 and the culture dish
Top end face 029 be separated by it is at regular intervals, by sample from sample-adding opening 011 add into the reservoir 022 of culture dish, and rotate on
Lid 01 is to drive coating brush 03 to rotate, so that coating brush 03 carries out automatic cut to the sample in reservoir 022；When the upper cover
01 turns to (as shown in Figure 8) when relatively described culture dish 02 is located at final position, the top 0142 of first twisted steel with
025 phase of limited block is supported, and the bottom 0143 of first twisted steel is located at the top of first groove 0271, and described
The bottom of two twisted steels 015 is located at the top of second groove 0272, and the bottom of the 3rd twisted steel 016 is positioned at described the
The top of three grooves 0273, the bottom of the 4th twisted steel 017 are located at the top of the 4th groove 0274, at this time coating brush
03 completes the scoring operation to sample, and the upper cover 01 can not be rotated further；By upper cover 01 to the axial direction close to culture dish 02
Direction pushes, until the top end face 029 of the convex block 013 and the culture dish is bonded to each other, first twisted steel at this time
Bottom 0143 is in first groove 0271, and the bottom of second twisted steel 015 is located in second groove 0272,
The bottom of 3rd twisted steel 016 is located in the 3rd groove 0273, and the bottom of the 4th twisted steel 017 is positioned at described
In 4th groove 0274, and the top 0142 of first twisted steel is right against the screw terminal of first thread groove 024, so that
The relatively described culture dish 02 of the upper cover 01 is obtained positioned at position (as shown in Figure 9) is screwed out, is rotated further upper cover 01, the first twisted steel
014 screws out along the first thread groove 024, so that upper cover 01 departs from culture dish 02, completes whole operation process；The microorganism is drawn
Trace device, safe and reliable, simple operation, and to upper cover 01 need not be opened during microorganism progress scoring operation.
Although embodiment of the present invention is disclosed as above, it is not limited in listed fortune in specification and embodiment
With it can be applied to various suitable the field of the invention completely, can be easily real for those skilled in the art
Now other modification, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited to
Specific details and shown here as the legend with description.
Claims (2)
- A kind of 1. microbial streaking device, it is characterised in that including：Culture dish, it is discoid in upward opening, and the inner bottom end face overlying of the culture dish is stamped culture medium, and offers One reservoir；Upper cover, it is in be mutually matched with the culture dish and downwardly open discoid, and one is offered on the end face of the upper cover It is open with the corresponding sample-adding of the reservoir；Coating brush, its top are connected on the end face of the upper cover；The coating brush is fixedly connected or is integrally formed with the upper cover；Limited block is provided with the top of the lateral wall of the culture dish, the first thread groove, the culture are offered on the limited block The lateral wall bottom of ware, which is provided with, keeps out bar, described to keep out the first groove offered on bar below the limited block；Institute State the first twisted steel for being provided with the madial wall of upper cover and being mutually matched with first thread groove, the end face inner side of the upper cover Equidistantly it is arranged at intervals with some convex blocks；When the relatively described culture dish of the upper cover is located at initial position, bar phase is kept out in the bottom of first twisted steel with described Support, to cause the top end face of the convex block and the culture dish to be set in a determining deviation；When the relatively described culture of the upper cover When ware is located at final position, top and the limited block of first twisted steel are mutually supported, and the bottom of first twisted steel End is positioned at the top of first groove；When the relatively described culture dish of the upper cover is positioned at back-out position, first screw thread The top of muscle is right against the screw terminal of first thread groove, and the bottom of first twisted steel is in first groove It is interior, to cause the convex block and the top end face of the culture dish to be bonded to each other setting；Locating piece is additionally provided with the top of the lateral wall of the culture dish, is offered on first twisted steel and the locating piece phase Matched notch；When the relatively described culture dish of the upper cover is located at initial position, notch described in the locating piece face；Described keep out article is additionally provided with the second groove being spaced apart with first groove, the 3rd groove and the 4th groove, The second twisted steel being spaced apart with first twisted steel, the 3rd twisted steel and are additionally provided with the madial wall of the upper cover Four twisted steels；When the relatively described culture dish of the upper cover is located at final position, the bottom of second twisted steel is positioned at described The top of second groove, the bottom of the 3rd twisted steel are located at the top of the 3rd groove, the bottom of the 4th twisted steel End is positioned at the top of the 4th groove.
- 2. microbial streaking device as claimed in claim 1, it is characterised in that the inner bottom end face of the culture dish it is upper in The side of the reservoir, which is provided with, puts area, when the relatively described culture dish of the upper cover is located at initial position and final position When, the coating brush is respectively positioned in the storing area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610226902.6A CN105695316B (en) | 2016-04-13 | 2016-04-13 | Microbial streaking device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610226902.6A CN105695316B (en) | 2016-04-13 | 2016-04-13 | Microbial streaking device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105695316A CN105695316A (en) | 2016-06-22 |
| CN105695316B true CN105695316B (en) | 2018-04-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610226902.6A Active CN105695316B (en) | 2016-04-13 | 2016-04-13 | Microbial streaking device |
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| CN (1) | CN105695316B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628281A (en) * | 2019-02-14 | 2019-04-16 | 严清园 | A kind of dedicated scoring devices of microbial culture dish |
| CN113881547B (en) * | 2021-10-19 | 2024-03-26 | 微尔创（武汉）医疗科技有限公司 | Anti-pollution scribing culture box |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101160240B1 (en) * | 2004-01-22 | 2012-06-26 | 메드벳 싸이언스 피티와이 엘티디 | Microbial streaking device |
| US8143053B2 (en) * | 2009-10-26 | 2012-03-27 | Biomerieux, Inc. | Lockable cell growth chamber with antilocking feature |
| JP5707724B2 (en) * | 2010-04-01 | 2015-04-30 | 大日本印刷株式会社 | Petri dish for medium preparation |
| CN102559476B (en) * | 2010-12-30 | 2015-10-14 | 苏州生物医学工程技术研究所 | A kind of microorganism seperation culture device |
| CN203582845U (en) * | 2013-09-29 | 2014-05-07 | 中国人民解放军第三军医大学 | Microorganism isolated culture device |
| CN205576141U (en) * | 2016-04-13 | 2016-09-14 | 中国科学院苏州生物医学工程技术研究所 | Microorganism mar device |
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2016
- 2016-04-13 CN CN201610226902.6A patent/CN105695316B/en active Active
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| CN105695316A (en) | 2016-06-22 |
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