CN113881547A - Anti-pollution marking-out culture box - Google Patents

Anti-pollution marking-out culture box Download PDF

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Publication number
CN113881547A
CN113881547A CN202111213268.XA CN202111213268A CN113881547A CN 113881547 A CN113881547 A CN 113881547A CN 202111213268 A CN202111213268 A CN 202111213268A CN 113881547 A CN113881547 A CN 113881547A
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culture
tank
shell
window
culture tank
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Granted
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CN202111213268.XA
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CN113881547B (en
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肖军
刘伟国
熊军
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Well Care Wuhan Medical Technology Co ltd
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Well Care Wuhan Medical Technology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention belongs to the field of structural design of microbial culture devices, and particularly relates to an anti-pollution lineation culture box. Comprises a culture dish, a box cover and a movable cover plate; a culture tank is arranged in the middle of the culture dish, a retention tank is arranged on the rear side of the culture tank, and a fixed adsorbent is arranged at the bottom of the retention tank; a protective shell matched with the culture tank in shape is arranged in the middle of the lower side of the box cover; the protective shell is tightly buckled on the culture tank; the left side and the right side in front of the protective shell or the culture tank are provided with shell walls, the shell walls on the left side and the right side extend forwards and enclose a window, and the movable cover plate is arranged in the window in an openable manner; the anti-pollution lineation culture box has the advantages of compact and simple structure, convenience in use, convenience in operation of the traditional culture dish, self-sealing structure, convenience in use and good sealing performance, and is provided with a special inoculation, lineation and sampling channel inside.

Description

Anti-pollution marking-out culture box
Technical Field
The invention belongs to the field of structural design of microbial culture devices, and particularly relates to an anti-pollution lineation culture box.
Background
The culture dish is the essential basic experiment utensil in fields such as medical test, biological cultivation, and mainly used constitutes relatively stable and suitable cultivation environment, keeps apart inside and outside environment simultaneously, prevents external disturbance and avoids inside culture to leak. In the culture process, various bacteria and the like need to be inoculated to substances such as a culture medium and the like in a culture dish by using tools such as an inoculating loop/an inoculating needle and the like, the inoculation process is limited by the structure and the operation mode of a traditional culture dish, the culture dish is often required to be opened to cause the internal culture medium, the biological material and the like to be exposed in an external space, when the external non-sterile environment exists, the biological material or the culture medium is easily polluted, the problem also exists in the streaking process, and the culture medium and the biological material can be adhered to the surface of the inoculating needle or the inoculating loop after streaking and need to be cleaned or recovered again, so the conventional inoculation culture process is complicated, the operation requirements on the operation environment and the operation are high, and the efficiency is low.
Disclosure of Invention
The invention aims to provide an anti-pollution streak culture box which has compact and reliable structure, good sealing performance and strong anti-pollution capacity, is beneficial to improving the sealing performance of inoculation and streak operation, reduces the operation content and improves the operation efficiency.
In order to achieve the purpose, the invention adopts the following technical scheme.
An anti-pollution lineation culture box comprises a culture dish 1, a box cover 2 and a movable cover plate 4;
the culture dish 1 is a shell with an open upper side; a culture tank 10 is arranged in the middle of the culture dish 1, a guide part 1c is arranged on the front side of the culture tank 10, a retention tank 11 is arranged on the rear side of the culture tank 10, and a fixed adsorption body 12 is arranged at the bottom of the retention tank 11;
the box cover 2 is buckled on the culture dish 1, and the box cover 2 is a shell with an open lower side; the middle part of the lower side of the box cover 2 is provided with a protective shell 20 matched with the shape of the culture tank 10;
the protective shell 20 is tightly buckled on the culture tank 10, an inlet 1a is arranged at the front side of the protective shell 20 or the wall of the culture tank 10, and an outlet 1b is arranged at the rear side of the protective shell 20 or the wall of the culture tank 10;
shell walls 21 are arranged on the front left and right sides of the protective shell 20 or the culture tank 10, the shell walls 21 on the left and right sides extend forwards and enclose a window 2b, and the movable cover plate 4 is arranged in the window 2b in an openable and closable manner;
the introduction port 1a faces the guide portion 1c and the window 2b, and the discharge port 1b faces the culture tank 10.
In a further modification or specific embodiment of the foregoing anti-contamination streak culture cassette, the introduction port 1a is located in a front side tank wall of the culture tank 10; the guide part 1c is arranged at the front side of the introduction port 1a, and the top of the guide part 1c is provided with a guide plane 1 e; the outlet port 1b is provided in the rear side wall of the culture tank 10, and the outlet port 1b and the retention tank 11 are connected by an inclined guide surface 1 d.
In a further modification or embodiment of the above-described contamination-preventive streak culture cassette, the front side of the protective case 20 is provided with a front bayonet 2a which is in abutment with the introduction port 1a, and the rear side of the protective case 20 is provided with a rear bayonet 2c which is in abutment with the discharge port 1 b.
In a further modification or specific embodiment of the foregoing contamination-preventive streak culture cassette, the casing wall 21 is provided on the protective case 20, and the lower side of the casing wall 21 abuts on the upper side of the guide portion 1 c;
the left and right side housing walls 21 form an introduction passage, the front side of which communicates with the window 2b and the rear side of which communicates with the introduction port 1 a.
In a further improvement or specific embodiment of the above-described anti-contamination streaking culture cassette, the cassette further includes a magnetic streaking head 3 movably disposed in the culture dish;
the magnetic scribing head 3 includes: a support cylinder 30 that can pass through the window 2b, the inlet 1a, and the outlet 1 b; a sliding scribe head 31 which is sleeved on the lower end of the support cylinder 30 and can slide up and down; a movable adsorption body 32 embedded at the upper end of the supporting cylinder 30; a compression spring 33 with two ends abutting against the movable adsorption body 32 and the sliding mark head 31; the movable adsorbent 32 and the fixed adsorbent 12 may be magnetically attracted to each other.
In a further improvement or specific embodiment of the anti-pollution lineation culture box, the opening at the lower end of the supporting cylinder 30 is provided with a step hole 30a, and the size of the upper end of the sliding lineation head 31 is larger than the aperture of the step hole 30a so as to be clamped on the step of the limiting step hole 30 a;
the upper end of the sliding marking head 31 is provided with a pressing groove 31a capable of accommodating the lower end of the compression spring 33, and the lower end of the sliding marking head 31 is provided with an inoculating loop 31 b.
In a further improvement or specific embodiment of the aforementioned anti-contamination streak culture cassette, the upper and lower ends of the movable cover plate 4 are provided with short shafts, and the movable cover plate 4 is rotatably connected to the upper and lower sides of the window 2b through the short shafts at both ends; a torsion spring is arranged between the movable cover plate 4 and the box cover 2 to cover the window 2 b; one side of the movable cover plate 4 facing the culture tank 10 is provided with a transversely extending extrusion strip 3a, and the middle part of the extrusion strip 3a is provided with an arc-shaped contact groove.
In a further development or embodiment of the aforementioned contamination-preventive streak culture cassette, the cassette bottom of the culture dish 1 is provided with a plurality of positioning posts 15 and positioning grooves 16.
In a further improvement or specific embodiment of the foregoing anti-contamination streak culture cassette, the cassette bottom of the culture dish 1 is provided with an adsorbent mounting groove 17, the adsorbent mounting groove 17 is aligned with the retention groove 11, and the fixed adsorbent 12 is embedded in the adsorbent mounting groove 17.
The beneficial effects are that:
the anti-pollution lineation culture box is compact and simple in structure and convenient to use, has the characteristic of convenient operation of a traditional culture dish, is additionally provided with a self-sealing structure, is internally provided with a special inoculation, lineation and sampling channel, can realize non-contact inoculation lineation operation by means of the cooperation of the magnetic adsorption device and the sampling piece, is additionally provided with a storage structure, is used for storing the culture box body after the sampling piece is used, does not need to be processed again, is convenient to use, and has good sealing property.
Drawings
FIG. 1 is a first schematic view of the structure of a contamination-preventive streak culture cassette;
FIG. 2 is a second schematic view showing the structure of the contamination preventive streak culture cassette;
FIG. 3 is a third schematic view of the structure of the contamination-preventive streak culture cassette;
FIG. 4 is an assembly view of the contamination-preventive streak culture cassette;
FIG. 5 is a schematic view of the structure of a magnetic scribing head;
wherein the reference numerals include:
the culture dish comprises a culture dish 1, an introduction port 1a, a discharge port 1b, a guide part 1c, an inclined guide surface 1d, a guide plane 1e, a culture tank 10, an indwelling tank 11, a fixed adsorption body 12, a positioning column 15, a positioning groove 16, an adsorption body installation groove 17, a box cover 2, a front bayonet 2a, a window 2b, a rear bayonet 2c, a protective shell 20, a shell wall 21, a magnetic scribing head 3, a supporting cylinder 30, a limit step hole 30a, a sliding scribing head 31, an extrusion groove 31a, an inoculating ring 31b, a movable adsorption body 32, a compression spring 33 and a movable cover plate 4.
Detailed Description
The present invention will be described in detail with reference to specific examples.
The anti-pollution lineation culture box is mainly used as a culture box part in a closed lineation culture device, has the characteristics of high closure, convenient use, simple structure and low production cost, and can realize lineation operation without opening the culture box by matching with an internal magnetic lineation head.
The basic structure is shown in fig. 1, fig. 2, fig. 3 and fig. 4, and comprises a culture dish 1, a box cover 2 and a movable cover plate 4;
wherein the culture dish 1 is a shell with an open upper side; a culture tank 10 is arranged in the middle of the culture dish 1, wherein a guide part 1c is arranged at the front side of the culture tank 10, a retention tank 11 is arranged at the rear side of the culture tank 10, and a fixed adsorbent 12 is arranged at the bottom of the retention tank 11;
wherein the box cover 2 is buckled on the culture dish 1, and the box cover 2 is a shell with an open lower side; the middle part of the lower side of the box cover 2 is provided with a protective shell 20 matched with the shape of the culture tank 10;
wherein the protective shell 20 is tightly buckled on the culture tank 10, the front side of the protective shell 20 or the wall of the culture tank 10 is provided with an inlet 1a, and the rear side of the protective shell 20 or the wall of the culture tank 10 is provided with an outlet 1 b;
wherein, the front left and right sides of the protective shell 20 or the culture tank 10 are provided with shell walls 21, the left and right shell walls 21 extend forwards and enclose a window 2b, and the movable cover plate 4 is arranged in the window 2b in an openable and closable way;
wherein the introduction port 1a is opposed to the guide portion 1c and the window 2b, and wherein the discharge port 1b is opposed to the culture tank 10.
Wherein the introduction port 1a is located in the front side wall of the culture tank 10; the guide part 1c is arranged at the front side of the introduction port 1a, and the top of the guide part 1c is provided with a guide plane 1 e; wherein the outlet port 1b is provided in the rear side tank wall of the culture tank 10, and wherein the outlet port 1b and the retention tank 11 are connected by an inclined guide surface 1 d.
In this embodiment, since the culture dish needs to be operated separately before and after the use and preparation process, so as to pour the culture medium, the box cover part does not need to be operated, and only is assembled at the end, in this embodiment, the complex structure is designed on the box cover 2, specifically:
a front bayonet 2a is provided on the front side of the protective case 20 to be in contact with the inlet 1a, and a rear bayonet 2c is provided on the rear side of the protective case 20 to be in contact with the outlet 1 b.
The front buckle, the introduction port, the outlet and the rear buckle are respectively utilized to form a marking head moving channel port, so that the marking head can be conveniently moved and positioned.
As a simpler structural design, the shell wall 21 is disposed on the protective casing 20, wherein the lower side of the shell wall 21 abuts against the upper side of the guiding portion 1 c; the left and right side housing walls 21 form an introduction passage, the front side of which communicates with the window 2b and the rear side of which communicates with the introduction port 1 a.
Particularly, in order to facilitate the scribing operation, the application also provides a magnetic scribing head 3 which can be matched with the culture box to carry out the actual scribing operation, and the magnetic scribing head is movably arranged and can be driven to act by an external magnetic adsorption device so as to realize the transfer of microorganisms or reagents and the scribing operation; specifically, the method comprises the following steps:
as shown in fig. 4, the magnetic scribing head 3 includes: a support cylinder 30 that can pass through the window 2b, the inlet 1a, and the outlet 1 b; a sliding scribe head 31 which is sleeved on the lower end of the support cylinder 30 and can slide up and down; a movable adsorption body 32 embedded at the upper end of the supporting cylinder 30; a compression spring 33 with two ends abutting against the movable adsorption body 32 and the sliding mark head 31; wherein the movable adsorbent 32 and the fixed adsorbent 12 can be magnetically adsorbed to each other.
In particular, to simplify the installation and use of the magnetic line marking head,
the lower opening of the supporting cylinder 30 is provided with a step hole 30a, wherein the size of the upper end of the sliding marking head 31 is larger than the aperture of the step hole 30a so as to be clamped on the step of the limiting step hole 30 a;
wherein the upper end of the sliding marking head 31 is provided with a pressing groove 31a which can accommodate the lower end of the compression spring 33, and the lower end of the sliding marking head 31 is provided with a connecting ring 31 b.
Wherein, the upper and lower ends of the movable cover plate 4 are provided with short shafts, and the movable cover plate 4 is rotatably connected with the upper and lower sides of the window 2b through the short shafts at the two ends; wherein, one side of the movable cover plate 4 facing the culture tank 10 is provided with a transversely extending extrusion strip 3a, and the middle part of the extrusion strip 3a is provided with an arc-shaped contact groove.
Further, the device is mainly used for matching with an automatic culture marking device, and in order to ensure the precision of marking and transferring operations, the culture box needs to be capable of keeping a relative position fixed on the device, and for this reason, in the embodiment, a plurality of positioning columns 15 and positioning grooves 16 are arranged at the box bottom of the culture dish 1. So as to utilize the positioning column and the positioning groove to fix the culture box, and keep the relative position thereof fixed.
In order to facilitate the installation of the fixed adsorbent, an adsorbent installation groove 17 is provided at the bottom of the culture dish 1, wherein the adsorbent installation groove 17 is directly opposite to the retention groove 11, and wherein the fixed adsorbent 12 is embedded in the adsorbent installation groove 17.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (9)

1. An anti-pollution lineation culture box is characterized by comprising a culture dish (1), a box cover (2) and a movable cover plate (4);
the culture dish (1) is a shell with an open upper side; a culture tank (10) is arranged in the middle of the culture dish (1), a guide part (1c) is arranged on the front side of the culture tank (10), a retention tank (11) is arranged on the rear side of the culture tank (10), and a fixed adsorption body (12) is arranged at the bottom of the retention tank (11);
the box cover (2) is buckled on the culture dish (1), and the box cover (2) is a shell with an open lower side; a protective shell (20) matched with the culture tank (10) in shape is arranged in the middle of the lower side of the box cover (2);
the protective shell (20) is tightly buckled on the culture tank (10), an inlet (1a) is arranged at the front side of the protective shell (20) or the wall of the culture tank (10), and an outlet (1b) is arranged at the rear side of the protective shell (20) or the wall of the culture tank (10);
shell walls (21) are arranged on the left side and the right side in front of the protective shell (20) or the culture tank (10), the shell walls (21) on the left side and the right side extend forwards and enclose a window (2b), and the movable cover plate (4) is arranged in the window (2b) in an openable and closable manner;
the introduction port (1a) is opposite to the guide part (1c) and the window (2b), and the outlet (1b) is opposite to the culture tank (10).
2. The contamination-preventive streak culture cassette according to claim 1, wherein said introduction port (1a) is provided in a front side wall of the culture vessel (10); the guide part (1c) is arranged at the front side of the introduction port (1a), and the top of the guide part (1c) is provided with a guide plane (1 e); the leading-out port (1b) is arranged on the rear side groove wall of the culture tank (10), and the leading-out port (1b) is connected with the retention groove (11) through an inclined guide surface (1 d).
3. The contamination-preventive streak culture cassette according to claim 2, wherein the front side of the protective case (20) is provided with a front bayonet (2a) which is butted against the introduction port (1a), and the rear side of the protective case (20) is provided with a rear bayonet (2c) which is butted against the discharge port (1 b).
4. The contamination-preventive streak culture cassette according to claim 3, wherein the shell wall (21) is provided on a protective case (20), and the lower side of the shell wall (21) abuts against the upper side of the guide portion (1 c);
the left and right side housing walls (21) form an introduction passage, the front side of which communicates with the window (2b), and the rear side of which communicates with the introduction port (1 a).
5. The contamination-preventive streaking culture cassette as claimed in claim 1, further comprising a magnetic streaking head (3) movably disposed in the culture dish;
the magnetic scribing head (3) comprises: a support cylinder (30) which can pass through the window (2b), the inlet (1a) and the outlet (1 b); a sliding marking head (31) which is sleeved at the lower end of the supporting cylinder (30) and can slide up and down; a movable adsorption body (32) embedded at the upper end of the supporting cylinder (30); a compression spring (33) with two ends abutting against the movable adsorption body (32) and the sliding mark head (31); the movable adsorbent (32) and the fixed adsorbent (12) can be mutually adsorbed by magnetism.
6. The contamination-preventive scribing culture box according to claim 5, wherein the lower end opening of the supporting cylinder (30) is provided as a stepped hole (30a), and the upper end of the sliding scribing head (31) is larger in size than the bore diameter of the stepped hole (30a) so as to be caught on the step of the limit stepped hole (30 a);
the upper end of the sliding marking head (31) is provided with an extrusion groove (31a) capable of accommodating the lower end of the compression spring (33), and the lower end of the sliding marking head (31) is provided with an inoculating ring (31 b).
7. The contamination-preventive streak culture cassette according to claim 1, wherein the upper and lower ends of the movable lid plate (4) are provided with short shafts, and the movable lid plate (4) is rotatably attached to the upper and lower sides of the window (2b) through the short shafts at both ends; a torsion spring is arranged between the movable cover plate (4) and the box cover (2) to cover the window (2 b);
one side of the movable cover plate (4) facing the culture tank (10) is provided with a transversely extending extrusion strip (3a), and the middle part of the extrusion strip (3a) is provided with an arc-shaped contact groove (3 c).
8. An anti-contamination streaking cassette as claimed in claim 1, in which the cassette bottom of the culture dish (1) is provided with a plurality of locating posts (15) and locating slots (16).
9. The anti-contamination streak culture cassette according to claim 1, wherein the bottom of the culture dish (1) is provided with an adsorbent mounting groove (17), the adsorbent mounting groove (17) is opposite to the retention groove (11), and the fixed adsorbent (12) is embedded in the adsorbent mounting groove (17).
CN202111213268.XA 2021-10-19 2021-10-19 Anti-pollution scribing culture box Active CN113881547B (en)

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CN113881547B CN113881547B (en) 2024-03-26

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114525199A (en) * 2022-01-19 2022-05-24 武汉旭德喜生物科技有限公司 Automatic marking-off culture apparatus culture dish locating component

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105695316A (en) * 2016-04-13 2016-06-22 中国科学院苏州生物医学工程技术研究所 Microorganism scratching device
CN209619349U (en) * 2019-01-18 2019-11-12 深圳市优圣康生物科技有限公司 A kind of antipollution culture dish
CN210261744U (en) * 2019-06-27 2020-04-07 魏小玲 Microbial cultivation box
CN210945585U (en) * 2019-06-11 2020-07-07 福建万邦清源环保科技有限公司 Anti-pollution culture dish convenient to operate

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105695316A (en) * 2016-04-13 2016-06-22 中国科学院苏州生物医学工程技术研究所 Microorganism scratching device
CN209619349U (en) * 2019-01-18 2019-11-12 深圳市优圣康生物科技有限公司 A kind of antipollution culture dish
CN210945585U (en) * 2019-06-11 2020-07-07 福建万邦清源环保科技有限公司 Anti-pollution culture dish convenient to operate
CN210261744U (en) * 2019-06-27 2020-04-07 魏小玲 Microbial cultivation box

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114525199A (en) * 2022-01-19 2022-05-24 武汉旭德喜生物科技有限公司 Automatic marking-off culture apparatus culture dish locating component

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