CN220724164U - PCR tube assembly and nuclear lifting and amplifying integrated machine using same - Google Patents

Abstract

The utility model provides a PCR tube component and a nuclear extraction and amplification all-in-one machine using the same, wherein the PCR tube component comprises: the tube tray can be fixedly connected with a tube bracket of the nuclear lifting and amplifying integrated machine; the multi-connected PCR tube comprises a plurality of accommodating tubes, wherein different reagents or samples are respectively accommodated in each accommodating tube, and the multi-connected PCR tube is arranged on the bearing surface of the tube tray; the pipe cover comprises a pressing cap, a plurality of pipe columns are arranged on the first end face of the pressing cap, each pipe column is arranged corresponding to each containing pipe respectively, the pipe opening of each containing pipe can be sealed after the pipe cover and the multi-connected PCR pipes are assembled, and an inserting connection groove is formed in the second end face of the pressing cap. The utility model realizes automatic cover opening and closing between the pipe cover and the multi-connected PCR pipe, does not need manual cover opening and closing, improves the working efficiency, has simple structure and low manufacturing cost, and is suitable for batch detection working conditions.

Description

PCR tube assembly and nuclear lifting and amplifying integrated machine using same

Technical Field

The utility model belongs to the technical field of nucleic acid detection instrument design, and particularly relates to a PCR tube assembly and a nuclear lifting and amplifying integrated machine using the same.

Background

With the development of PCR technology, integrated equipment for extracting and amplifying nucleic acid has become a great trend in the development of molecular diagnosis, and the sample size for detecting nucleic acid has increased rapidly, and the requirements on the degree of automation and the detection efficiency of nucleic acid detection are also raised. Sample in-out nuclear amplification (i.e., nucleic acid extraction amplification) integrated devices are becoming increasingly popular in the market.

The development of the nuclear lifting and amplification integrated equipment provides requirements for the PCR tube, and the PCR tube can pre-store PCR reaction system reagents, is convenient to transport and is required to be easy to automatically integrate and realize instruments. At present, when a PCR instrument is used for PCR, a conventional qPCR standard tube with the volume of 0.1ml or 0.2ml or a conventional qPCR eight-connecting tube is mostly adopted, and before the conventional qPCR tube is added into a reagent system for PCR reaction, a manual cover is needed, the cover action is difficult to be completed through an automatic structure, the instrument is extremely complicated, the reliability cannot be ensured, and the automatic operation of the instrument is not suitable.

Disclosure of Invention

The utility model provides a PCR tube assembly and a nuclear lifting and amplifying all-in-one machine using the same, which can solve the technical problems that a PCR tube adopted in the prior art nuclear lifting and amplifying all-in-one machine needs to be manually covered before a reagent system is added for PCR reaction, and the structure of the prior PCR tube is not suitable for automatic operation.

In order to solve the above problems, the present utility model provides a PCR tube assembly comprising: the tube tray can be fixedly connected with a tube bracket of the nuclear lifting and amplifying integrated machine; the multi-connected PCR tube comprises a plurality of accommodating tubes, wherein different reagents or samples are respectively accommodated in each accommodating tube, and the multi-connected PCR tube is arranged on the bearing surface of the tube tray in a sitting manner; the pipe cover comprises a pressing cap, a plurality of pipe columns are arranged on a first end face of the pressing cap, each pipe column is arranged corresponding to each containing pipe respectively, the pipe mouth of each containing pipe can be sealed after the pipe cover and the multi-connected PCR pipes are assembled, a plug-in connection groove is formed in a second end face of the pressing cap, and the plug-in connection groove is used for being matched and plugged with a plug of a gun head adapter of the nuclear lifting and amplifying all-in-one machine.

In some embodiments, the second end surface of the press cap is further provided with a weight-reducing groove, and the weight-reducing groove is positioned at two opposite areas of the plug-in connection groove.

In some embodiments, the press cap is a cavity structure with one side open, reinforcing ribs are arranged in the cavity structure at opposite intervals, a gap between two reinforcing ribs forms the plug-in connection groove, and a gap between two reinforcing ribs and a wall body of the press cap forms the weight-reducing groove.

In some embodiments, the tube tray is configured with a detent on one side wall.

In some embodiments, each of the tubular strings is a hollow cylinder.

The utility model also provides a nuclear lifting and amplifying integrated machine, and the PCR tube assembly is applied.

In some embodiments, the nuclear lift amplification all-in-one machine includes a gun head adapter including a main body having one end formed with a cartridge head on which an O-ring is assembled.

In some embodiments, the shape of the cartridge is further adapted to house a piercing head, a pipette tip.

The PCR tube assembly and the nuclear extraction and amplification integrated machine using the same provided by the utility model have the following beneficial effects:

the pipe cover is characterized in that an inserting connection groove is formed in the top end face of the pipe cover, namely, the end face of one side far away from the multi-connected PCR pipe, when the pipe cover is required to be covered at the pipe orifice of the multi-connected PCR pipe, a plug of the control gun head adapter is inserted into the inserting connection groove, position control of the pipe cover is achieved through movement of the control gun head adapter, automatic cover opening and cover closing between the pipe cover and the multi-connected PCR pipe are achieved, manual cover closing is not needed, and working efficiency is improved.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those skilled in the art from this disclosure that the drawings described below are merely exemplary and that other embodiments may be derived from the drawings provided without undue effort.

The structures, proportions, sizes, etc. shown in the present specification are shown only for the purposes of illustration and description, and are not intended to limit the scope of the utility model, which is defined by the claims, so that any structural modifications, changes in proportions, or adjustments of sizes, which do not affect the efficacy or the achievement of the present utility model, should fall within the ambit of the technical disclosure.

FIG. 1 is a schematic diagram showing a three-dimensional structure of a PCR module according to an embodiment of the present utility model;

FIG. 2 is a cross-sectional view of the PCR assembly of FIG. 1;

FIG. 3 is a schematic perspective view of the tube cover in FIG. 1;

FIG. 4 is a top view of FIG. 1;

FIG. 5 is a schematic view of a gun head adapter according to another embodiment of the present utility model;

fig. 6 is a schematic view showing the state of the adaptor for gun head, the puncture head, the tube cap and the pipette tip of the present utility model.

The reference numerals are expressed as:

1. a tube cover; 101. a plug-in connection groove; 102. pressing the cap; 103. a tubular column; 104. reinforcing rib plates; 2. a tube tray; 21. a positioning groove; 3. a multiplex PCR tube; 4. a gun head adapter; 401. a main body; 402. an O-ring; 5. a puncture head; 6. pipette tip.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the utility model, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.

It should be understood that the term "and/or" as used herein is merely one relationship describing the association of the associated objects, meaning that there may be three relationships, e.g., a and/or B, may represent: a exists alone, A and B exist together, and B exists alone. In addition, the character "/" herein generally indicates that the front and rear associated objects are an "or" relationship.

The relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present utility model unless it is specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective parts shown in the drawings are not drawn in actual scale for convenience of description. Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but should be considered part of the specification where appropriate. In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further discussion thereof is necessary in subsequent figures.

In the description of the present utility model, it should be understood that the azimuth or positional relationships indicated by the azimuth terms such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal", and "top, bottom", etc., are generally based on the azimuth or positional relationships shown in the drawings, merely to facilitate description of the present utility model and simplify the description, and these azimuth terms do not indicate and imply that the apparatus or elements referred to must have a specific azimuth or be constructed and operated in a specific azimuth, and thus should not be construed as limiting the scope of protection of the present utility model; the orientation word "inner and outer" refers to inner and outer relative to the contour of the respective component itself.

Spatially relative terms, such as "above … …," "above … …," "upper surface at … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial location relative to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the exemplary term "above … …" may include both orientations of "above … …" and "below … …". The device may also be positioned in other different ways (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

In addition, the terms "first", "second", etc. are used to define the components, and are only for convenience of distinguishing the corresponding components, and the terms have no special meaning unless otherwise stated, and therefore should not be construed as limiting the scope of the present utility model.

Referring to fig. 1 to 6, according to an embodiment of the present utility model, there is provided a PCR tube assembly including:

a tube tray 2 which can be fixedly connected with a tube bracket (not shown in the figure) of the nuclear lifting and amplifying integrated machine;

the multiple PCR tubes 3 include a plurality of accommodating tubes, each accommodating tube accommodates different reagents or samples, the multiple PCR tubes 3 are seated on a bearing surface (not labeled in the figure, and referring to the bearing surface shown in fig. 1, that is, an upper surface of a bearing space of the tube tray 2) of the tube tray 2, and the multiple PCR tubes are reasonably selected according to actual detection requirements, and in a specific embodiment of the present utility model, four tubes are provided, that is, four accommodating tubes are provided at the same time;

the pipe cover 1 comprises a press cap 102 which is used as a structural main body of the pipe cover 1, a plurality of pipe columns 103 are arranged on a first end face (taking the direction shown in fig. 1 as a reference, namely, a bottom end face) of the press cap 102, the number of the pipe columns 103 is matched and corresponds to the number of containing pipes of the multi-connected PCR pipes, each pipe column 103 is respectively arranged corresponding to each containing pipe so as to be capable of sealing a pipe orifice of each containing pipe after the pipe cover 1 and the multi-connected PCR pipes 3 are assembled, and a plug-in connection groove 101 is formed on a second end face (taking the direction shown in fig. 2 as a reference, namely, a top end face) of the press cap 102, and the plug-in connection groove 101 is used for being matched and plugged in with a plug of a gun head adapter 4 of the nuclear lifting and amplifying all-in machine; it can be understood that, in the original state of the multi-connected PCR tube 3, the tube opening of each accommodating tube is subjected to sealing treatment so as to encapsulate each reagent preset in each accommodating tube therein, and when the multi-connected PCR tube 3 is transferred into the nuclear lifting and amplifying integrated machine for subsequent use, the sealing membrane of the tube opening of each accommodating tube should be pierced, and then the tube cover 1 is covered on the multi-connected PCR tube 3.

In the technical scheme, the plug-in connection groove 101 is formed in the top end face of the pipe cover 1, namely, the end face of one side far away from the multi-connected PCR pipe 3, when the pipe cover 1 is required to be covered at the pipe orifice of the multi-connected PCR pipe 3, the plug of the control gun head adapter 4 is inserted into the plug-in connection groove 101, the position control of the pipe cover 1 is realized by controlling the movement of the gun head adapter 4, and further, the automatic cover opening and the cover closing between the pipe cover 1 and the multi-connected PCR pipe 3 are realized, the manual cover opening and closing are not required, the working efficiency is improved, and in addition, the pipe cover 1 disclosed by the utility model is simple in structure, low in manufacturing cost and suitable for batch detection working conditions.

Referring to fig. 3, in some embodiments, the second end surface of the press cap 102 further has a weight-reducing groove (not shown in the drawings), where the weight-reducing groove is located at two opposite sides of the plug-in connection groove 101, and in a preferred embodiment, the weight-reducing groove is symmetrically disposed about the plug-in connection groove 101. By arranging the weight-reducing grooves, on one hand, the whole weight of the pipe cover 1 can be relatively light, and smooth driving control of the gun head adapter 4 is facilitated; on the other hand, the manufacturing cost is further reduced.

Referring further to fig. 3, in some embodiments, the press cap 102 has a cavity structure with an opening at one side (for example, an opening at the top side), and reinforcing ribs 104 are disposed in the cavity structure at opposite intervals, a gap between two reinforcing ribs 104 forms the plug-in connection groove 101, and a gap between two reinforcing ribs 104 and a wall body of the press cap 102 forms the weight-reducing groove.

In this technical scheme, the aforementioned cartridge connection groove 101 and the weight-reducing groove are formed by the two reinforcing rib plates 104, so that the structure is simple, and the structural rigidity of the whole gland 1 can be improved.

The cross-sectional shape of the aforementioned mating recess 101 should match the cross-sectional shape of the gun head adapter 4, for example, in one embodiment, the cross-sectional shape of the mating recess 101 is circular, and the cross-section of the mating gun head adapter 4 is also circular, so that the two achieve a reliable mating connection through static friction forces formed by mating contact surfaces that are shaped.

In some embodiments, a positioning groove 21 is configured on a side wall of one side of the tube tray 2, referring specifically to fig. 4, the positioning groove 21 is a semicircular groove, and the positioning groove can be matched with a positioning column on a tube support of the nuclear lifting and amplifying integrated machine, so that the position of the tube tray 2 is reliable, stable and accurate.

In some embodiments, each pipe column 103 is a hollow cylinder, and it can be understood that the bottom surface of the hollow cylinder is a closed end, so that each accommodating tube is closed when the tube cover 1 and the multi-connected PCR tube 3 are covered, and the material consumption of the tube cover 1 can be further reduced and the manufacturing cost can be reduced by adopting the structure of the hollow cylinder.

According to the embodiment of the utility model, a nuclear lifting and amplifying integrated machine is further provided, and the PCR tube assembly is applied.

In a preferred embodiment, the nuclear lifting and amplifying integrated machine comprises a gun head adapter 4, wherein the gun head adapter 4 comprises a main body 401, one end of the main body 401 is provided with a plug-in head (not marked in the figure), and an O-ring 402 is assembled on the plug-in head.

In this technical solution, the O-ring 402 is disposed on the insertion head, so that good tightness between the O-ring 402 and the corresponding insertion object is achieved, for example, the gun head adapter 4 is further suitable for inserting the puncture head 5 and the pipette gun head 6, especially when the O-ring 402 is inserted into the pipette gun head 6, the tightness of the matching position between the O-ring 402 and the corresponding insertion object is ensured, and the accuracy of the amount of the transferred liquid is ensured; in addition, the aforementioned O-ring 402 can also objectively increase the static friction force between the O-ring and the inserting object, so as to ensure the reliable and stable inserting between the O-ring and the inserting object.

In the present utility model, when the nucleic acid extraction and amplification integrated machine is used for automatic nucleic acid extraction, a pipetting mechanism (generally, the pipetting mechanism comprises a pump, a gun head adapter 4 between the pump and the gun head, and a pipetting gun head 6). The gun head adapter 4 is used for grabbing the pipetting gun head 6, and when the pipette head adapter is connected with the pipetting gun head 6, tightness between the pipette head adapter and the gun head is required to be ensured so as to ensure accuracy and precision of pipetting operation. The design of the tube cover 1 skillfully utilizes the structure of the gun head adapter 4, so that the gun head adapter is utilized to the maximum extent, and the tube cover 1, the film sealing puncture head 5 of the multi-connected PCR tube 3 and the pipette head 6 can be simultaneously matched, thereby greatly reducing the integration difficulty and the cost of the instrument.

The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the utility model. The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that it will be apparent to those skilled in the art that modifications and variations can be made without departing from the technical principles of the present utility model, and these modifications and variations should also be regarded as the scope of the utility model.

Claims (8)

1. A PCR tube assembly, comprising:

the tube tray (2) can be fixedly connected with a tube bracket of the nuclear lifting and amplifying integrated machine;

the multi-connected PCR tube (3) comprises a plurality of accommodating tubes, wherein different reagents or samples are respectively accommodated in each accommodating tube, and the multi-connected PCR tube (3) is arranged on the bearing surface of the tube tray (2);

the pipe cover (1) comprises a pressing cap (102), a plurality of pipe columns (103) are arranged on the first end face of the pressing cap (102), the pipe columns (103) are arranged corresponding to the accommodating pipes respectively, the pipe openings of the accommodating pipes can be sealed after the pipe cover (1) and the multi-connected PCR pipes (3) are assembled, a plug-in connection groove (101) is formed in the second end face of the pressing cap (102), and the plug-in connection groove (101) is used for being matched and plugged with a plug of a gun head adapter (4) of the nuclear lifting and amplifying all-in-one machine.

2. The PCR tube assembly as claimed in claim 1, wherein the second end face of the press cap (102) further has a weight-reducing groove in opposite regions of both sides of the cartridge connecting groove (101).

3. The PCR tube assembly as claimed in claim 2, wherein the pressure cap (102) has a cavity structure with one side open, reinforcing ribs (104) are arranged in the cavity structure at opposite intervals, a gap between the two reinforcing ribs (104) forms the insertion connection groove (101), and a gap between the two reinforcing ribs (104) and a wall body of the pressure cap (102) forms the weight reduction groove.

4. PCR tube assembly according to claim 1, characterized in that a positioning groove (21) is constructed on one side wall of the tube tray (2).

5. The PCR tube assembly according to claim 1, wherein each of the tube columns (103) is a hollow cylinder.

6. A nuclear lifting and amplifying integrated machine, which is characterized in that the PCR tube assembly as claimed in any one of claims 1 to 5 is applied.

7. The nuclear lifting and amplifying integrated machine according to claim 6, comprising a gun head adapter (4), wherein the gun head adapter (4) comprises a main body (401), a plug is formed at one end of the main body (401), and an O-ring (402) is assembled on the plug.

8. The integrated machine for nuclear lifting and amplification according to claim 7, wherein the shape of the insertion head is further suitable for inserting a puncture head (5) and a pipetting gun head (6).