CN116121039A

CN116121039A - Sample detection consumable and sample detection method

Info

Publication number: CN116121039A
Application number: CN202111343338.3A
Authority: CN
Inventors: 陈海东; 解亚平; 曾波; 吴常; 戴立忠
Original assignee: Sansure Biotech Inc
Current assignee: Sansure Biotech Inc
Priority date: 2021-11-13
Filing date: 2021-11-13
Publication date: 2023-05-16

Abstract

The invention relates to a sample detection consumable and a sample detection method. The sample detection consumable comprises: the shell is provided with a mixing cavity, a sample adding port and a mounting port, wherein the sample adding port is communicated with the mixing cavity, and the mounting port is communicated with the mixing cavity; the reaction tube is detachably arranged at the mounting port; and an air extraction assembly disposed in the housing and configured to operatively extract air from the reaction tube such that liquid from the mixing chamber is drawn into the reaction tube. Thus, the sample is added into the mixing cavity from the sample adding port, so that the sample and the reagent liquid preset in the mixing cavity are mixed and subjected to cracking reaction. Then, the operation bleed subassembly is in order to take out the air in the reaction tube, and then makes the liquid inflow reaction tube in the mixing chamber to carry out PCR to the liquid in the reaction tube and detect, need not the operator and carry out actions such as opening light lid and extrusion to a plurality of tubular consumptive materials, simplified the operation flow greatly, reduced the risk of error occurrence, reduced the study cost.

Description

Sample detection consumable and sample detection method

Technical Field

The invention relates to the technical field of biological detection, in particular to a sample detection consumable and a sample detection method.

Background

The development of isothermal amplification technology has been very rapid in recent years due to the relatively simple requirements for practical operation and instrumentation. In general, it is necessary to contain a sample and a reagent with consumables to accomplish extraction and amplification of nucleic acids, so that the sample can be detected by a isothermal amplification apparatus.

However, the consumable materials used in the prior art are more, such as a cracking tube, a dilution tube, a reaction tube and the like, and an operator needs to perform actions of opening and closing a cover, extruding and the like on each consumable material in the use process, so that the operation is very complicated, errors are easy to occur in the operation process, and the learning cost is increased.

Disclosure of Invention

Based on the above, it is necessary to provide a sample detection consumable and a sample detection method for improving the above-mentioned defects, aiming at the problems of complicated operation and high learning cost of operators caused by a large number of consumable types in the prior art.

A sample testing consumable comprising:

the shell is provided with a mixing cavity, a sample adding port and a mounting port, wherein the sample adding port is communicated with the mixing cavity, and the mounting port is communicated with the mixing cavity;

the reaction tube is detachably arranged at the mounting opening; and

And the air extraction assembly is arranged on the shell and is configured to extract the air in the reaction tube in an operable way so as to suck the liquid in the mixing cavity into the reaction tube.

In one embodiment, the sample testing consumable further comprises a sample collector detachably mounted at the loading port.

In one embodiment, the sample detection consumable further comprises a reagent liquid cover, wherein the reagent liquid cover and the sample collector are alternatively and detachably arranged at the sample adding port.

In one embodiment, the housing has a connection portion disposed around the sample application port, and the sample collector and the reagent cap are both threadably connected to the connection portion.

In one embodiment, the reagent liquid cover is provided with a containing cavity for containing reagent liquid and an opening communicated with the containing cavity, and the opening is sealed by a sealing film;

the shell is provided with a puncture part arranged around the sample adding port, and the puncture part is positioned between the connecting part and the sample adding port; when the reagent liquid cover is in threaded connection with the connecting part, the puncture part punctures the sealing film.

In one embodiment, the housing has a liquid channel, one end of the liquid channel is communicated with the mixing cavity, and the other end of the liquid channel is communicated with the mounting port.

In one embodiment, the housing further comprises a suction chamber in communication with the mounting port;

the air extraction assembly comprises a piston, the piston is in sliding fit with the inner wall of the suction cavity, and air in the reaction tube can be extracted into the suction cavity in the process that the piston slides along the inner wall of the suction cavity.

In one embodiment, the housing has a gas passage, one end of which communicates with the mounting port, and the other end of which communicates with the suction chamber.

In one embodiment, the pumping chamber has opposed closed and open ends, the gas passage extending through the closed end, one end of the piston extending into the pumping chamber from the open end.

In one embodiment, the sample detection consumable further comprises a sealing ring sleeved outside the piston to seal a gap between the piston and an inner wall of the suction cavity.

In one embodiment, the air extraction assembly comprises a valve core and a piston, wherein the valve core is provided with a communication channel, a piston cavity and a communication hole;

the valve core is movably connected to the shell and comprises a first position and a second position in the process of moving relative to the shell; when the valve core is positioned at the first position, at least one of the mixing cavity and the mounting port is not communicated with the communication channel; when the valve core is positioned at the second position, the communication channel is communicated with both the mixing cavity and the mounting port, and the communication hole is communicated with the mounting port;

the piston is in sliding fit with the inner wall of the piston cavity, and when the valve core is located at the second position, air in the reaction tube can be pumped into the piston cavity through the communication hole in the process that the piston slides along the inner wall of the piston cavity.

In one embodiment, the valve core has a stop portion, and the housing has a stop mating portion, and the stop portion abuts against the stop mating portion when the valve core moves to the second position.

In one embodiment, the reaction tube is threadably connected to the mounting port.

A sample testing method employing the sample testing consumable of any of the above embodiments, comprising the steps of:

adding a sample into the mixing cavity through the sample adding port;

shaking the sample detection consumable to uniformly mix the sample with the reagent liquid preset in the uniformly mixing cavity;

operating the air extraction assembly to extract air in the reaction tube so that liquid in the mixing cavity flows into the reaction tube;

and detecting the liquid in the reaction tube.

According to the sample detection consumable and the sample detection method, the sample is added into the mixing cavity through the sample adding port, so that the sample is mixed with the reagent liquid preset in the mixing cavity, and a cracking reaction occurs. Then, the operation bleed subassembly to take out the air in the reaction tube, and then make the liquid inflow reaction tube of mixing intracavity, so that follow-up carry out PCR (polymerase chain reaction) fluorescence detection to the liquid in the reaction tube, need not the operator to carry out actions such as opening light lid and extrusion to a plurality of pipe class consumptive materials, simplified the operation flow greatly, reduced the introduction of the risk and the pollution that appear wrong, reduced study cost.

Drawings

FIG. 1 is a cross-sectional view of a sample testing consumable (sample collector mounted at a sample application port of a housing) according to one embodiment of the present invention;

FIG. 2 is a cross-sectional view of a sample testing consumable (a reagent cap is mounted at a sample inlet of a housing) according to an embodiment of the present invention;

FIG. 3 is a cross-sectional view of a sample testing consumable in accordance with another embodiment of the present invention (with a sample collector mounted at the sample application port of the housing and in an open position);

FIG. 4 is a cross-sectional view of a sample testing consumable in accordance with another embodiment of the present invention (the sample collector is mounted at the sample application port of the housing and in a closed state);

FIG. 5 is a cross-sectional view of a sample testing consumable (a reagent cap is mounted at a sample application port of a housing) according to another embodiment of the present invention;

FIG. 6 is a cross-sectional view of the sample testing consumable shown in FIG. 5 (showing a pusher bar);

FIG. 7 is a cross-sectional view of the sample sensing consumable shown in FIG. 6 (with the cartridge in a first position);

FIG. 8 is a cross-sectional view of the sample sensing consumable shown in FIG. 7 (with the cartridge in a second position);

FIG. 9 is a cross-sectional view of the sample sensing consumable shown in FIG. 8 (pulling the piston to move relative to the cartridge);

FIG. 10 is a flowchart illustrating steps of a method for detecting a sample according to an embodiment of the present invention.

Detailed Description

In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the invention, whereby the invention is not limited to the specific embodiments disclosed below.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present invention, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.

In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.

Referring to fig. 1 and 2, a sample testing consumable according to an embodiment of the present invention includes a housing 10, a reaction tube 20, and an air extraction assembly.

The housing 10 has a mixing chamber 11, a sample inlet 12 and a mounting port 13. The sample adding port 12 is communicated with the mixing cavity 11, and the mounting port 13 is communicated with the mixing cavity 11. The reaction tube 20 is detachably mounted to the mounting port 13. The air extraction assembly is disposed on the housing 10 and is configured to operatively extract air from the reaction tube 20 such that the air pressure in the reaction tube 20 is lower than the air pressure in the mixing chamber 11, i.e., an air pressure difference is formed between the mixing chamber 11 and the reaction tube 20, so that the liquid in the mixing chamber 11 is sucked into the reaction tube 20 under the air pressure difference, i.e., the transfer of the liquid is realized.

Above-mentioned sample detects consumptive material, when in actual use, adds the sample by the application of sample mouth 12 in mixing chamber 11 for sample mixes and takes place the cleavage reaction with the reagent liquid of predetermineeing in mixing chamber 11. Then, the air extraction assembly is operated to extract the air in the reaction tube 20, so that the liquid in the mixing cavity 11 flows into the reaction tube 20, the subsequent PCR (polymerase chain reaction) fluorescence detection of the liquid in the reaction tube 20 is conveniently performed, the actions of opening the light cover, extruding and the like of a plurality of tube consumables are not required by an operator, the operation flow is greatly simplified, the risks of errors and the introduction of pollution are reduced, and the learning cost is reduced.

It should be noted that, since the liquid in the mixing chamber 11 is sucked into the reaction tube 20 by using the air sucking device to suck the air in the reaction tube 20, the amount of the liquid sucked into the reaction tube 20 can be controlled by controlling the amount of the air sucked by the air sucking device, so that the accurate control of the amount of the liquid sucked into the reaction tube 20, i.e., the quantitative transfer of the liquid can be realized.

It should be noted that, a reagent solution is preset in the mixing chamber 11, and the reagent solution may be a lysis solution. The reaction tube 20 is also provided with a reagent solution, which may be a PCR reaction solution.

In the embodiment, the reaction tube 20 is in threaded connection with the mounting port 13 of the housing 10, on one hand, the reaction tube 20 is detachably connected with the mounting port 13 of the housing 10, the reaction tube 20 can be conveniently detached, different nucleic acid detecting instruments can be matched, and the compatibility is strong; on the other hand, the sealing of the gap between the reaction tube 20 and the inner wall of the mounting port 13 is realized, and the phenomenon that external air enters the reaction tube 20 through the gap between the reaction tube 20 and the inner wall of the mounting port 13, so that liquid in the mixing cavity 11 cannot be sucked into the reaction tube 20 is avoided. More specifically, the mounting port 13 has a second female screw, and the reaction tube 20 has a second male screw that can be screwed with the second female screw.

In an embodiment of the present invention, the sample testing consumable further comprises a sample collector 40 (see FIG. 1), the sample collector 40 being detachably mounted at the loading port 12. Thus, in practical use, the sample collector 40 is mounted at the sample loading port 12 of the housing 10, the sample collector 40 is used for collecting a sample to be tested (such as saliva), and the collected sample to be tested is introduced into the mixing chamber 11 through the sample loading port 12.

In particular embodiments, the sample testing consumable further comprises a reagent fluid cap 50 (see FIG. 2), the reagent fluid cap 50 and the sample collector 40 alternatively and detachably mounted at the loading port 12. Thus, after the sample to be detected in the sample collector 40 is introduced into the mixing cavity 11, the sample collector 40 is detached, and the reagent liquid cover 50 is mounted at the sample adding port 12 of the housing 10, so that the reagent liquid in the reagent liquid cover 50 flows into the mixing cavity 11 from the sample adding port 12, and the reagent liquid and the sample are uniformly mixed. Alternatively, the reagent liquid in the reagent liquid cover 50 may be a diluent.

In some embodiments, the housing 10 has a connection portion 15 disposed around the loading port 12, and the sample collector 40 and the reagent cap 50 are both threadably connected to the connection portion 15. Further, the connection portion 15 has a first external thread, and the sample collector 40 and the reagent cap 50 each have a first internal thread that can be screwed with the first external thread.

In particular, in the embodiment, the sample collector 40 has a collection chamber 41, and a collection port 42 and an outflow port 43 communicating with the collection chamber 41. When the sample collector 40 is screwed to the connecting portion 15, the flow outlet 43 communicates with the sample application port 12, so that the sample introduced into the collection chamber 41 from the collection port 42 sequentially passes through the flow outlet 43 and the sample application port 12 to enter the mixing chamber 11.

Referring to fig. 3 and 4, in another embodiment, the collecting opening 42 of the sample collector 40 may be closed by a cover 44, and a push rod 45 is connected to a side of the cover 44 facing the collecting cavity 41. The outflow opening 43 of the sample collector 40 is provided with a first plug 431. When the cover 44 is pressed, the ejector rod 45 can be driven to push out the first plug 431, so that the sample in the collection cavity 41 can flow out of the outflow port 43 and further enter the mixing cavity 11 through the sample adding port 12. More specifically, the ejector 45 has a liquid pushing portion 451 that engages with the inner wall of the collection chamber 41, and the sample is accommodated in a space between the liquid pushing portion 451 and the first stopper 431. When the cap 44 is pressed, the plunger 45 pushes out the first stopper 431, and the pusher 451 pushes out the sample from the outlet 43.

Further, a cover 44 is movably coupled to the sample collector 40 and is capable of opening or closing the collection port 42. Alternatively, the cover 44 may be attached to the sample collector 40 by a flexible portion 441, and the cover 44 may be switched between a state of opening the collection port 42 and a state of closing the collection port 42 by deformation of the flexible portion 441.

Referring to fig. 2, in one embodiment, the reagent solution cover 50 has a receiving chamber 51 for receiving the reagent solution and an opening 52 communicating with the receiving chamber 51, and the opening 52 is sealed by a sealing film, so as to prevent the reagent solution in the receiving chamber 51 from leaking out of the opening 52. The housing 10 has a lancing portion 16 disposed around the loading port 12, the lancing portion 16 being located between the connecting portion 15 and the loading port 12. When the reagent cap 50 is screwed to the connecting portion 15, the piercing portion 16 pierces the sealing film sealing the opening 52, so that the reagent in the accommodating cavity 51 can sequentially enter the mixing cavity 11 through the opening 52 and the sample inlet 12, so as to be fully mixed with the sample in the mixing cavity 11, and a cleavage reaction occurs.

In order to facilitate the puncturing of the sealing film, further, the height of the puncturing part 16 at one side of the sample loading port 12 is different from the height at the other side of the sample loading port 12, so that the puncturing part 16 can puncture the sealing film easily in the process of screwing the reagent liquid cover 50.

The reagent liquid cap 50 is not limited to the one in which the sealing film is pierced to fill the reagent liquid. Referring to fig. 5 to 7, in another embodiment, the reagent liquid cover 50 has a receiving chamber 51 for receiving the reagent liquid and oppositely disposed inlet and outlet ports each communicating with the receiving chamber 51. The inlet is provided with a second plug 53 to close the inlet by the second plug 53. The outlet is provided with a third plug 54 to close the outlet by the third plug 54. Thus, when the reagent cap 50 is screwed onto the connecting portion 15, a push rod 55 can be inserted from the inlet of the reagent cap 50 to push the reagent in the second plug 53, the third plug 54 and the accommodating cavity 51 from the outlet into the mixing cavity 11.

Referring to fig. 1 and 2, in some embodiments of the present invention, the housing 10 further includes a suction chamber 14 in communication with the mounting port 13. The air extraction assembly comprises a piston 30, the piston 30 is in sliding fit with the inner wall of the suction cavity 14, and air in the reaction tube 20 can be pumped into the suction cavity 14 in the process of sliding the piston 30 along the inner wall of the suction cavity 14, so that the air pressure in the reaction tube 20 is lower than the air pressure in the mixing cavity 11, namely, an air pressure difference is formed between the mixing cavity 11 and the reaction tube 20, and liquid in the mixing cavity 11 is sucked into the reaction tube 20 under the action of the air pressure difference, namely, liquid transfer is realized. Thus, the amount of liquid sucked into the reaction tube 20 can be controlled by controlling the moving distance of the piston 30, thereby realizing accurate control of the amount of liquid sucked into the reaction tube 20, i.e., realizing quantitative transfer of liquid.

In particular, in the embodiment, the housing 10 has a liquid passage 17, one end of the liquid passage 17 communicates with the mixing chamber 11, and the other end of the liquid passage 17 communicates with the mounting port 13. Thus, when the piston 30 is pulled, the air in the reaction tube 20 is pumped into the pumping cavity 14, so that the air pressure in the reaction tube 20 is smaller than the air pressure in the mixing cavity 11, and the liquid in the mixing cavity 11 flows into the reaction tube 20 from the liquid channel 17 under the action of the air pressure difference, so that the quantitative transfer of the liquid is realized.

In order to ensure that a pressure difference is generated between the mixing chamber 11 and the reaction tube 20 when the piston 30 is pulled, the position of the liquid passage 17 penetrating one end of the mixing chamber 11 is located below the liquid level of the liquid in the mixing chamber 11. In order to avoid that the liquid in the reaction tube 20 flows back to the mixing chamber 11, the position of the liquid passage 17 penetrating the mounting opening 13 is located at the top of the reaction tube 20.

In particular to the embodiment, the housing 10 has a gas passage 18, one end of the gas passage 18 communicates with the mounting port 13, and the other end of the gas passage 18 communicates with the suction chamber 14. Thus, when the piston 30 is pulled, the gas in the reaction tube 20 mounted in the mounting port 13 enters the suction chamber 14 through the gas passage 18, so that the gas pressure in the reaction tube 20 is lower than the gas pressure in the mixing chamber 11.

In order to prevent the liquid in the reaction tube 20 from entering the pumping chamber 14 through the gas channel 18, further, one end of the gas channel 18 penetrating the mounting opening 13 is positioned at the top of the reaction tube 20, thereby preventing the liquid in the reaction tube 20 from entering the pumping chamber 14 through the gas channel 18.

In particular embodiments, the pumping chamber 14 has opposed closed ends 141 and an open end 142 through which the gas channel 18 extends, and one end of the piston 30 extends from the open end 142 into the pumping chamber 14 to ensure that gas in the reaction tube 20 is drawn into the pumping chamber 14 as the piston 30 is pulled toward the open end 142.

Further, the sample testing consumable further comprises a sealing ring 60, and the sealing ring 60 is sleeved on the outer side of the piston 30 to seal a gap between the piston 30 and the inner wall of the suction cavity 14. In this way, air is prevented from entering the pumping chamber 14 from the gap between the piston 30 and the inner wall of the pumping chamber 14, so that air in the reaction tube 20 cannot be pumped into the pumping chamber 14. Alternatively, the sealing ring 60 may be a rubber ring, and of course, other sealing materials may be used as long as the sealing effect is achieved.

In the embodiment, the mixing chamber 11 and the pumping chamber 14 are respectively located at two longitudinal ends of the casing 10, and the mounting opening 13 is located in the middle of the casing 10, so that the sample collector 40 or the reagent liquid cover 50 mounted at the sample adding opening 12, the reaction tube 20 mounted at the mounting opening 13, and the piston 30 mounted at the pumping chamber 14 are sequentially arranged along the longitudinal direction of the casing 10, which is beneficial to matching with various instruments to complete nucleic acid extraction, amplification, detection, and the like.

It should be noted that the air extraction assembly is not limited to the structure of the above embodiment. Referring to fig. 8 and 9, in other embodiments, the pumping assembly includes a valve spool 31 and a piston 30. The valve body 31 has a communication passage 311, a piston chamber 314, and a communication hole 312. The spool 31 is movably coupled to the housing 10 and includes a first position (see fig. 8) and a second position (see fig. 9) during movement relative to the housing 10. When the valve body 31 is located at the first position, at least one of the mixing chamber 11 and the mounting port 13 is not communicated with the communication passage 311, so that the mixing chamber 11 is not communicated with the reaction tube 20 mounted to the mounting port 13. When the valve element 31 is located at the second position, the communication passage 311 communicates with both the mixing chamber 11 and the mounting port 13, so that the mixing chamber 11 communicates with the reaction tube 20 mounted to the mounting port 13 through the communication passage 311. At this time, the communication hole 312 communicates with the mounting port 13 such that the piston chamber 314 communicates with the reaction tube 20 mounted to the mounting port 13 through the communication hole 312.

When the valve core 31 is located at the second position, air in the reaction tube 20 can be pumped into the piston cavity 314 through the communication hole 312 in the process that the piston 30 slides along the inner wall of the piston cavity 314, so that the air pressure in the reaction tube 20 is lower than the air pressure in the mixing cavity 11, namely, an air pressure difference is formed between the mixing cavity 11 and the reaction tube 20, and liquid in the mixing cavity 11 enters the reaction tube 20 through the communication channel 311 under the action of the air pressure difference, namely, transfer of the liquid is realized. Thus, the amount of liquid sucked into the reaction tube 20 can be controlled by controlling the moving distance of the piston 30, thereby realizing accurate control of the amount of liquid sucked into the reaction tube 20, i.e., realizing quantitative transfer of liquid.

In particular, in the embodiment, the valve core 31 has a stop portion 313, and the housing 10 has a stop mating portion 19. When the valve element 31 moves from the first position to the second position, the stopper 313 abuts against the stopper fitting 19, so that the valve element 31 stays in the second position.

It should be noted that, the retarding force of the movement of the piston 30 relative to the valve core 31 is greater than the retarding force of the movement of the valve core 31 relative to the housing 10. Thus, when the valve element 31 is in the first position, a pulling force is applied to the piston 30, and the piston 30 does not move relative to the valve element 31 at first, but the piston 30 moves relative to the housing 10 together with the valve element 31 until it moves to the second position. When the valve body 31 is moved to the second position, if a pulling force is continuously applied to the piston 30, the piston 30 is moved relative to the valve body 31 (the valve body 31 is fixed relative to the housing 10), thereby exhausting the air in the reaction tube 20.

Further, the sample testing consumable further includes a sealing ring 60, and the sealing ring 60 is sleeved on the outer side of the piston 30 to seal a gap between the piston 30 and the inner wall of the piston cavity 314. In this manner, air is prevented from entering the piston chamber 314 from the gap between the piston 30 and the inner wall of the piston chamber 314, so that air in the reaction tube 20 cannot be drawn into the piston chamber 314. Alternatively, the sealing ring 60 may be a rubber ring, and of course, other sealing materials may be used as long as the sealing effect is achieved.

In the embodiment, the mixing cavity 11 is located at the top of the casing 10, the mounting opening 13 is located at the bottom of the casing 10, and the valve core 31 is disposed at a position between the mixing cavity 11 and the mounting opening 13 of the casing 10, so that the sample collector 40 or the reagent liquid cover 50 mounted at the sample adding opening 12, the valve core 31 and the reaction tube 20 mounted at the mounting opening 13 are sequentially arranged along the vertical direction of the casing 10, which is beneficial to the processing and forming of the casing 10.

Based on the sample detection consumable, the invention also provides a sample detection method using the sample detection consumable in any embodiment, and the sample detection method comprises the following steps:

s10, adding the sample to be detected into the mixing cavity 11 through the sample adding port 12. Optionally, the sample to be detected may be saliva, and a lysis solution may be preset in the mixing chamber 11. Specifically, the sample collector 40 is mounted at the sample loading port 12 of the housing 10, so that the sample to be detected in the sample collector 40 is introduced into the mixing chamber 11 through the sample loading port 12.

S20, shaking the sample detection consumable material to enable the sample and the lysis solution preset in the mixing cavity 11 to be uniformly mixed, and further enable the sample and the lysis solution to undergo a lysis reaction.

S30, operating the air extraction assembly to extract air in the reaction tube 20, so that the air pressure in the reaction tube 20 is reduced, and the liquid in the mixing cavity 11 flows into the reaction tube 20, so that quantitative transfer of the liquid is realized. In particular to the embodiment shown in the drawings, the particular action of operating the pumping assembly is pulling the piston 30 to the right.

S40, detecting the liquid in the reaction tube 20. Alternatively, the liquid in the reaction tube 20 is subjected to fluorescent PCR detection.

In an embodiment, the method further includes the steps of:

the sample collector 40 is detached, and the reagent liquid cover 50 is mounted at the sample loading port 12 of the housing 10, so that the piercing part 16 pierces the sealing film on the reagent liquid cover 50 or pushes out the second plug 53 and the third plug 54 through the push rod, and then the reagent liquid in the reagent liquid cover 50 flows into the mixing cavity 11 through the sample loading port 12. Alternatively, the reagent liquid in the reagent liquid cover 50 may be a diluent. The sample detection consumable and the sample detection method have the following advantages:

in actual use, the sample is added into the mixing cavity 11 through the sample adding port 12, so that the sample and the reagent solution preset in the mixing cavity 11 are mixed and subjected to a cracking reaction. Then, the air extraction assembly is operated to pump air in the reaction tube 20 into the suction cavity 14, so that liquid in the uniform mixing cavity 11 flows into the reaction tube 20, the liquid in the reaction tube 20 is detected PCR (polymerase chain reaction) later, actions such as opening a light cover and extruding a plurality of tube consumables are not needed by an operator, the operation flow is greatly simplified, the risk of errors is reduced, and the learning cost is reduced;

because the liquid in the mixing cavity 11 is pumped into the reaction tube 20 by pulling the piston 30, the amount of the liquid pumped into the reaction tube 20 can be controlled by controlling the moving amount of the piston 30, so that the accurate control of the amount of the liquid pumped into the reaction tube 20, namely the quantitative transfer of the liquid, is realized;

when the sample collector 40 is in threaded connection with the connecting part 15, the outflow port 43 is communicated with the sample adding port 12, so that the sample entering the collecting cavity 41 from the collecting port 42 sequentially enters the mixing cavity 11 through the outflow port 43 and the sample adding port 12, namely, the collection of the sample to be detected is realized; alternatively, by pressing the cap body, the ejector rod is caused to eject the first plug and push the sample in the sample collector 40 into the mixing chamber 11.

When the reagent liquid cover 50 is in threaded connection with the connecting part 15, the piercing part 16 pierces the sealing film of the sealing opening 52, so that the reagent liquid in the accommodating cavity 51 can sequentially enter the mixing cavity 11 through the opening 52 and the sample adding port 12 to be fully mixed with the sample in the mixing cavity 11, and the reagent liquid is added; alternatively, the second plug and the third plug are pushed open by the push rod, and the reagent liquid in the reagent liquid cover 50 is pushed into the mixing chamber 11.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

Claims

1. A sample testing consumable, comprising:

the shell (10) is provided with a mixing cavity (11), a sample adding port (12) and a mounting port (13), wherein the sample adding port (12) is communicated with the mixing cavity (11), and the mounting port (13) is communicated with the mixing cavity (11);

a reaction tube (20) detachably mounted to the mounting port (13); and

And a pumping assembly provided to the housing (10) and configured to pump air inside the reaction tube (20) so that the liquid inside the mixing chamber (11) is sucked into the reaction tube (20).

2. The sample testing consumable according to claim 1, further comprising a sample collector (40), said sample collector (40) being detachably mounted at said loading port (12).

3. The sample detection consumable according to claim 2, further comprising a reagent liquid cover (50), the reagent liquid cover (50) and the sample collector (40) being alternatively and detachably mounted at the sample application port (12).

4. A sample testing consumable according to claim 3, wherein the housing (10) has a connection (15) arranged around the loading port (12), the sample collector (40) and the reagent liquid cap (50) both being screwed to the connection (15).

5. The sample detection consumable according to claim 4, wherein the reagent liquid cover (50) has a housing chamber (51) for housing a reagent liquid and an opening (52) communicating with the housing chamber (51), the opening (52) being sealed by a sealing film;

the housing (10) has a piercing portion (16) disposed around the loading port (12), the piercing portion (16) being located between the connecting portion (15) and the loading port (12); when the reagent liquid cover (50) is screwed to the connecting portion (15), the piercing portion (16) pierces the sealing film.

6. Sample detection consumable according to claim 1, characterized in that the housing (10) has a liquid channel (17), one end of the liquid channel (17) being in communication with the mixing chamber (11), the other end of the liquid channel (17) being in communication with the mounting opening (13).

7. The sample detection consumable according to claim 1, characterized in that the housing (10) further comprises a suction cavity (14) communicating with the mounting port (13);

the air extraction assembly comprises a piston (30), the piston (30) is in sliding fit with the inner wall of the suction cavity (14), and air in the reaction tube (20) can be pumped into the suction cavity (14) in the process that the piston (30) slides along the inner wall of the suction cavity (14).

8. The sample detection consumable according to claim 7, characterized in that the housing (10) has a gas channel (18), one end of the gas channel (18) being in communication with the mounting port (13), the other end of the gas channel (18) being in communication with the suction chamber (14).

9. The sample detection consumable according to claim 7, characterized in that the pumping chamber (14) has opposite closed end (141) and open end (142), the gas channel (18) extending through the closed end (141), one end of the piston (30) protruding from the open end (142) into the pumping chamber (14).

10. The sample detection consumable according to claim 7, further comprising a sealing ring (60), the sealing ring (60) being sleeved outside the piston (30) to seal a gap between the piston (30) and an inner wall of the pumping chamber (14).

11. The sample detection consumable according to claim 1, wherein the pumping assembly comprises a valve core (31) and a piston (30), the valve core (31) having a communication channel (311), a piston cavity (314) and a communication hole (312);

the valve core (31) is movably connected with the shell (10) and comprises a first position and a second position in the process of moving relative to the shell (10); when the valve core (31) is positioned at the first position, at least one of the mixing cavity (11) and the mounting port (13) is not communicated with the communication channel (311); when the valve core (31) is positioned at the second position, the communication channel (311) is communicated with both the mixing cavity (11) and the mounting port (13), and the communication hole (312) is communicated with the mounting port (13);

the piston (30) is in sliding fit with the inner wall of the piston cavity (314), and when the valve core (31) is located at the second position, air in the reaction tube (20) can be pumped into the piston cavity (314) through the communication hole (312) in the process that the piston (30) slides along the inner wall of the piston cavity (314).

12. Sample detection consumable according to claim 11, characterized in that the cartridge (31) has a stop (313), the housing (10) has a stop mating portion (19), the stop (313) being in abutment with the stop mating portion (19) when the cartridge (31) is moved to the second position.

13. Sample detection consumable according to claim 1, characterized in that the reaction tube (20) is screwed to the mounting port (13).

14. A sample testing method using the sample testing consumable of any one of claims 1 to 13, comprising the steps of:

adding a sample into the mixing cavity (11) through the sample adding port (12);

shaking the sample detection consumable to mix the sample with a reagent solution preset in the mixing cavity (11);

operating the air extraction assembly to extract air from the reaction tube (20) so that liquid in the mixing chamber (11) flows into the reaction tube (20);

detecting the liquid in the reaction tube (20).