CN116606731B - Drawing type nucleic acid detection quantitative transfer consumable - Google Patents
Drawing type nucleic acid detection quantitative transfer consumable Download PDFInfo
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- CN116606731B CN116606731B CN202310896038.0A CN202310896038A CN116606731B CN 116606731 B CN116606731 B CN 116606731B CN 202310896038 A CN202310896038 A CN 202310896038A CN 116606731 B CN116606731 B CN 116606731B
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- 238000012546 transfer Methods 0.000 title claims abstract description 45
- 238000001514 detection method Methods 0.000 title claims abstract description 44
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 29
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 29
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 208
- 239000012530 fluid Substances 0.000 claims abstract description 49
- 230000006835 compression Effects 0.000 claims abstract description 44
- 238000007906 compression Methods 0.000 claims abstract description 44
- 238000006243 chemical reaction Methods 0.000 claims abstract description 29
- 238000003780 insertion Methods 0.000 claims description 43
- 230000037431 insertion Effects 0.000 claims description 43
- 238000011084 recovery Methods 0.000 claims description 12
- 230000001133 acceleration Effects 0.000 claims description 5
- 238000009423 ventilation Methods 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 4
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 238000007689 inspection Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 38
- 238000013461 design Methods 0.000 description 21
- 238000010586 diagram Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The application discloses a drawing type quantitative transfer consumable for nucleic acid detection, belonging to the fields of medical examination and inspection instruments and molecular diagnosis and detection instruments; the technical key points are as follows: comprising the following steps: the device comprises a detection device body, a reaction tube, a piston control rod and a liquid adjusting moving component; the piston control rod can move up and down in the vertical movement space of the piston; the liquid adjusting moving component can move along the middle moving space; in an initial state, the lower port of the quantitative liquid storage space is communicated with the upper port of the diversion quantitative liquid storage pipeline, and the upper port of the quantitative liquid storage space is communicated with the vertical movement space of the piston; after the liquid adjusting moving member moves for a certain distance, the lower port of the quantitative liquid storage space can be communicated with the lower port of the fluid channel, and the upper port can be communicated with the lower port of the third vertical channel; the upper port of the air compression space is communicated with the vertical movement space of the piston, and the horizontal port is communicated with the horizontal port of the fluid channel.
Description
Technical Field
The application relates to the field of medical examination and inspection instruments and molecular diagnosis and detection instruments, in particular to a drawing type nucleic acid detection quantitative transfer consumable.
Background
For infectious diseases caused by viruses, PCR fluorescence detection is a conventional detection method.
For PCR fluorescence detection, related consumables are one of the key factors for ensuring the quality of PCR detection:
first, whether the sample is contaminated during transfer. For example: CN216525846U, CN112877193A, CN115141745A, CN115125129a, etc., and the whole process from nucleic acid extraction to PCR reaction is fully automatic, thus avoiding external pollution.
Second, quantitative control of the sample during transfer. The amplification reaction of PCR is essentially a chemical reaction, and excessive or insufficient sample size re-transfer results in failure of the amplification reaction. Meanwhile, the application range of sample size transfer is wide and narrow for different viruses. For narrow test ranges, accurate quantitative transfer of the sample is required.
For most consumables, the first point requirement is essentially fulfilled, while the second point requirement is relatively poor. Typically, as in CN112877192a, the sample can better prevent the sample in the sample tube from leaking out and avoid infection when flowing into the reaction tube from the sample tube, but in the transfer process, the solution in the sample tube needs to be introduced into the flow channel through the sample puncture tube by inversion, shaking or centrifugation, and then the sample solution in the flow channel flows into the reaction tube by inversion, shaking or centrifugation. The operations of inversion, shaking and centrifugation are extremely subjective, so that the solutions flowing into the reaction tube from the sample tube are inevitably greatly different under different forces, and accurate transfer cannot be achieved.
Therefore, the above-mentioned problems are a technical problem to be solved in the IVD detection industry.
Disclosure of Invention
The application aims to overcome the defects of the prior art and provide a drawing type nucleic acid detection quantitative transfer consumable.
The technical scheme of the application is as follows:
a pull type nucleic acid detection quantitative transfer consumable comprising: the device comprises a detection device body, a reaction tube, a piston control rod and a liquid adjusting moving component;
wherein, transfer liquid moving member includes: the quantitative liquid storage space and the air compression space are respectively provided with ports on the upper surface and the lower surface of the liquid adjusting moving component; the air compression space is provided with ports on the upper surface and one side surface of the liquid adjusting moving component, which are respectively called an upper port of the air compression space and a horizontal port of the air compression space;
wherein, detection device body includes: a solution introduction zone, a solution transfer zone;
the solution introduction zone comprises: a piercing member, a solution introduction port; the puncture member is used for puncturing the lower end part of the collecting pipe body; the solution inlet is used for introducing the solution flowing out of the sample collection tube;
the solution transfer zone comprises: the middle part moves the space, the piston moves the space vertically, the third vertical channel, the outflow channel, the fluid channel; the solution guide inlet is communicated with the lower surface of the middle moving space through a diversion quantitative liquid storage pipeline; the piston vertical movement space is communicated with the upper surface of the middle movement space; the lower port of the third vertical channel is communicated with the middle moving space; one end of the outflow channel is communicated with the upper port of the third vertical channel, and the other end of the outflow channel is communicated with the reaction tube; the fluid channel is arranged on the side wall of the middle moving space, ports are arranged on the side surface and the lower surface of the middle moving space, and the fluid channel is divided into a horizontal port called the fluid channel and a lower port called the fluid channel;
wherein the piston control rod can move up and down in the vertical movement space of the piston (the shape of the piston control rod and the piston control rod are adaptive);
wherein the liquid adjusting moving member can move along the middle moving space;
in an initial state, the lower port of the quantitative liquid storage space is communicated with the upper port of the diversion quantitative liquid storage pipeline, and the upper port of the quantitative liquid storage space is communicated with the vertical movement space of the piston;
after the liquid adjusting moving member moves for a certain distance, the lower port of the quantitative liquid storage space can be communicated with the lower port of the fluid channel, and the upper port of the quantitative liquid storage space can be communicated with the lower port of the third vertical channel; the upper port of the air compression space is communicated with the vertical movement space of the piston, and the horizontal port of the air compression space is communicated with the horizontal port of the fluid channel.
Further, the reaction tube includes: auxiliary insertion rod, liquid inlet insertion rod, liquid outlet insertion rod, liquid inlet pipeline, liquid storage space and liquid outlet pipeline; the liquid outlet insertion rod, the auxiliary insertion rod and the liquid inlet insertion rod are sequentially arranged in parallel from top to bottom; the liquid inlet insertion rod is provided with a liquid inlet which is communicated with the liquid inlet pipeline; the liquid outlet inserting rod is provided with a liquid outlet which is communicated with the liquid outlet pipeline; the liquid inlet pipeline is communicated with the lower end of the liquid storage space, and the liquid outlet pipeline is communicated with the upper end of the liquid storage space.
Further, the pull type nucleic acid detection quantitative transfer consumable further comprises: a filter element and a solution recovery zone; a solution recovery area is arranged on the detection device body; a ventilation port is arranged above the solution recovery area, and a filter element is arranged at the ventilation port;
the liquid outlet inserting rod connecting hole is provided with a through hole, so that when the reaction tube is inserted into the detection device body, the solution recovery area is communicated with the liquid outlet of the liquid outlet inserting rod.
Further, from top to bottom, the right side of the detection device body is provided with: go out liquid insert pole connecting hole, supplementary insert pole connecting hole, feed liquor insert pole connecting hole, go out liquid insert pole supplementary insert pole feed liquor insert pole insert in proper order go out liquid insert pole connecting hole, supplementary insert pole connecting hole, feed liquor insert pole connecting hole.
Further, the pull type nucleic acid detection quantitative transfer consumable further comprises: a sample collection tube; the sample acquisition tube includes: a collecting tube body; the lower part of collection pipe body includes: a lower end portion and a protruding side wall portion protruding from the lower end portion.
Further, the solution inlet is communicated with the lower surface of the middle moving space through a first bottom channel and a first vertical channel (namely, a diversion quantitative liquid storage pipeline is formed by the first bottom channel and the first vertical channel together), the solution inlet is communicated with the first bottom channel, and the first bottom channel is communicated with a lower port of the first vertical channel; the upper port of the first vertical channel is communicated with the middle moving space.
Further, the piston vertical movement space is communicated with the upper surface of the middle part movement space through a second vertical channel, the lower port of the second vertical channel is communicated with the middle part movement space, and the upper port of the second vertical channel is communicated with the lower port of the piston vertical movement space.
Further, the air compression space is an L-shaped space.
Further, the drawing type nucleic acid detection quantitative transfer consumable comprises the following steps when in solution transfer:
step1: placing a sample collection tube into a solution introduction zone of a testing device body;
step2: the piston control rod is pulled upwards, and the flow direction of the solution is as follows: sample collection tube-solution introduction port-first bottom channel-first vertical channel-quantitative liquid storage space-second vertical channel-piston vertical movement space;
step3: moving the liquid adjusting moving member for a distance to align the upper port of the quantitative liquid storage space with the lower port of the third vertical channel, and align the lower port of the quantitative liquid storage space with the lower port of the fluid channel; meanwhile, the upper port of the air compression space is aligned with the lower port of the second vertical channel, and the horizontal port of the air compression space is communicated with the horizontal port of the fluid channel;
step4: the piston control rod pushes downwards:
the flow direction of the gas is: an air compression space-a fluid channel-a quantitative liquid storage space-a third vertical channel;
the flow direction of the solution is: quantitative liquid storage space, third vertical channel, outflow channel, liquid inlet of liquid insertion rod, liquid inlet pipeline, liquid storage space, liquid outlet pipeline and liquid outlet insertion rod.
Further, the solution introduction zone further comprises: a sample tube guiding space for providing a guiding action for the sample collection tube; the puncture member is disposed at the bottom of the sample tube guiding space.
Further, the second vertical channel, the air compression space, the fluid channel, the third vertical channel and the quantitative liquid storage space all adopt pipelines with the same diameter.
Further, y, r 2 、h、V 1、 V 3 The requirements are as follows: pi r 2 2 y≥V 1 ρgh /（P 0 +ρgh）+ V 3 ；
Wherein r is 2 Representing the radius of the vertical movement space of the piston;
wherein y represents the distance from the solution to the vertical movement space of the piston when the piston control rod is pulled upwards;
wherein V is 1 Representing the sum of the volume of the fluid passage and the volume of the air compression space;
wherein ρ represents the density of the solution;
wherein g represents gravitational acceleration;
wherein h represents the height of the quantitative liquid storage space;
wherein P is 0 Represents 1 standard atmospheric pressure;
wherein V is 3 Representing the sum of the volume of the quantitative liquid storage space and the volume of the third vertical channel.
The application has the beneficial effects that:
first, the basic concept of the present application is: the application provides the idea of quantitative transfer of nucleic acid detection. The key design is that the solution transferring area, the piston control rod and the liquid adjusting moving component are matched.
1.1 The piston control rod can move up and down in the vertical movement space of the piston (the shape of the piston control rod and the piston control rod are adaptive);
1.2 The liquid adjusting moving component can move along the middle moving space; in an initial state, the lower port of the quantitative liquid storage space is communicated with the upper port of the diversion quantitative liquid storage pipeline, and the upper port of the quantitative liquid storage space is communicated with the vertical movement space of the piston;
1.3 After the liquid adjusting moving member moves for a certain distance, the lower port of the quantitative liquid storage space can be communicated with the lower port of the fluid channel, and the upper port of the quantitative liquid storage space can be communicated with the lower port of the third vertical channel; the upper port of the air compression space is communicated with the vertical movement space of the piston, and the horizontal port of the air compression space is communicated with the horizontal port of the fluid channel.
Second, a second application point of the present application is that: in the workflow of the device. The key design is as follows:
step1: placing a sample collection tube into a solution introduction zone of a testing device body;
step2: the piston control rod is pulled upwards, and the flow direction of the solution is as follows: sample collection tube-solution introduction port-first bottom channel-first vertical channel-quantitative liquid storage space-second vertical channel-piston vertical movement space;
step3: moving the liquid adjusting moving member for a distance to align the upper port of the quantitative liquid storage space with the lower port of the third vertical channel, and align the lower port of the quantitative liquid storage space with the lower port of the fluid channel; meanwhile, the upper port of the air compression space is aligned with the lower port of the second vertical channel, and the horizontal port of the air compression space is communicated with the horizontal port of the fluid channel;
step4: the piston control rod pushes downwards:
the flow direction of the gas is: an air compression space-a fluid channel-a quantitative liquid storage space-a third vertical channel;
the flow direction of the solution is: quantitative liquid storage space, third vertical channel, outflow channel, liquid inlet of liquid insertion rod, liquid inlet pipeline, liquid storage space, liquid outlet pipeline and liquid outlet insertion rod.
Different amounts of solution transfer are achieved by adjusting the distance the plunger lever in Step4 is pushed down.
Third, a third application point of the present application is that: the design of the sample collection tube.
3.1 The lower part of the collecting tube body is provided with a lower end part and a protruding side wall part protruding from the lower end part, and the lower part is matched with the lower structure of the solution introducing area of the detecting device body: the protruding side wall part is matched with the puncture member and the solution inlet to prevent the solution from contacting with the outside air and prevent the solution from being polluted.
3.2 In order to prevent the solution from falling from the periphery of the puncture member, a solution diversion pipeline is arranged at one side of the puncture member, and the solution diversion pipeline is communicated with the solution inlet.
Fourth, a fourth application of the present application is that: y, r 2 、h、V 1、 V 3 The requirements are as follows: pi r 2 2 y≥V 1 ρgh /（P 0 +ρgh）+ V 3 ；
Wherein r is 2 Representing the radius of the vertical movement space of the piston;
wherein y represents the distance from the solution to the vertical movement space of the piston when the piston control rod is pulled upwards;
wherein V is 1 Representing the sum of the volume of the fluid passage and the volume of the air compression space;
wherein ρ represents the density of the solution;
wherein g represents gravitational acceleration;
wherein h represents the height of the quantitative liquid storage space;
wherein P is 0 Represents 1 standard atmospheric pressure;
wherein V is 3 Representing the sum of the volume of the quantitative liquid storage space and the volume of the third vertical channel.
This is a critical design method that allows the amount of solution in the vertical movement space of the piston to meet the requirements for quantitative pipetting.
Drawings
The application is described in further detail below in connection with the embodiments in the drawings, but is not to be construed as limiting the application in any way.
FIG. 1 is a schematic three-dimensional design of the pull type nucleic acid detecting quantitative transfer consumable of example 1.
FIG. 2 is a schematic diagram of a three-dimensional exploded design of the pull type nucleic acid detecting quantitative transfer consumables of example 1.
FIG. 3 is a schematic representation of the three-dimensional design of the sample collection tube of example 1.
FIG. 4 is a schematic representation of the three-dimensional design of the sample collection tube of example 1 at another viewing angle.
FIG. 5 is a schematic representation of the three-dimensional design of the reaction tube of example 1.
FIG. 6 is a schematic view showing the three-dimensional design of the reaction tube of example 1 at another view angle.
Fig. 7 is a left end face design schematic diagram of the detection device body of embodiment 1.
Fig. 8 is a three-dimensional design schematic diagram of the left end face of the detection device body of embodiment 1 at another view angle.
Fig. 9 is a schematic front elevation design of the detection device body of embodiment 1.
Fig. 10 is a three-dimensional design schematic of the detection device body of embodiment 1.
Fig. 11 is a schematic three-dimensional design of the liquid adjusting moving member of embodiment 1.
Fig. 12 is a schematic three-dimensional design of the liquid adjusting moving member of embodiment 1 at another view angle.
Fig. 13 is a right side design schematic of the liquid adjusting moving member of embodiment 1.
FIG. 14 is a schematic diagram of a first embodiment of a solution transfer operation.
FIG. 15 is a schematic diagram of a second solution transfer operation.
Fig. 16 is a schematic diagram showing the design of the right end face of the detecting device body of embodiment 1.
The reference numerals are explained as follows:
the device comprises a drawing type nucleic acid detection quantitative transfer consumable 100, a sample collection tube 200, a detection device body 300, a reaction tube 400, a piston control rod 500, a liquid adjusting moving member 600 and a filter element 700;
a collection tube cap 201, a collection tube body 202, a lower end 2021, and a protruding sidewall 2022;
the detection device body 300, a sample tube guiding space 301, a puncture member 302, a solution introducing port 303, a first bottom channel 304, a first vertical channel 305, a middle moving space 306, a second vertical channel 307, a piston vertical moving space 308, a third vertical channel 309, an outflow channel 3010, a fluid channel 3011, a liquid outlet insertion rod connecting hole 3012, an auxiliary insertion rod connecting hole 3013, a liquid inlet insertion rod connecting hole 3014, a solution recovery area 3015, a longitudinal horizontal section 3011-1, a vertical section 3011-2, and a transverse horizontal section 3011-3;
auxiliary inserting rod 401, liquid inlet inserting rod 402, liquid outlet inserting rod 403, liquid inlet pipe 404, liquid storage space 405 and liquid outlet pipe 406;
a liquid adjusting moving member 600, a constant liquid storage space 601, an air compression space 602, and a horizontal projection 603.
Detailed Description
The objects, technical solutions and advantages of the present application will become more apparent by the following detailed description of the present application with reference to the accompanying drawings. It should be understood that the description is only illustrative and is not intended to limit the scope of the application. In addition, in the following description, descriptions of well-known structures and techniques are omitted so as not to unnecessarily obscure the present application.
< example 1 > A pull type nucleic acid detecting quantitative transfer consumable
< general Structure >
Fig. 1-2 schematically illustrate the general construction of a pull type nucleic acid detecting quantitative transfer consumable 100. A pull type nucleic acid detecting quantitative transfer consumable 100 comprising: sample collection tube 200, detection device body 300, reaction tube 400, plunger control rod 500, liquid adjustment moving member 600, and cartridge 700.
Fig. 3 illustrates the structural design of sample collection tube 200. Sample collection tube 200, comprising: a collection tube cap 201 and a collection tube body 202; the collection tube cap 201 is integrally connected to the collection tube body 202. Fig. 4 schematically illustrates the structure of the lower portion of the collection tube body 202. The lower portion of the collection tube body 202 includes: a lower end portion 2021, and a protruding side wall portion 2022 protruding from the lower end portion 2021.
Fig. 5 to 6 schematically show the structural design of the reaction tube 400. The reaction tube 400 includes: auxiliary inserting rod 401, liquid inlet inserting rod 402, liquid outlet inserting rod 403, liquid inlet pipe 404, liquid storage space 405 and liquid outlet pipe 406; wherein, the liquid outlet insertion rod 403, the auxiliary insertion rod 401 and the liquid inlet insertion rod 402 are sequentially arranged in parallel from top to bottom; the liquid inlet insertion rod 402 is provided with a liquid inlet which is communicated with the liquid inlet pipeline 404; the liquid outlet insertion rod 403 is provided with a liquid outlet, and the liquid outlet is communicated with the liquid outlet pipeline 406; the liquid inlet pipe 404 is communicated with the lower end of the liquid storage space 405, and the liquid outlet pipe 406 is communicated with the upper end of the liquid storage space 405. That is, the solution enters the liquid inlet pipe 404 from the liquid inlet of the liquid inlet insertion rod 402, is mixed with the solution pre-stored in the liquid storage space 405, and then the excessive solution can flow out from the liquid outlet pipe 406 and the liquid outlet of the liquid outlet insertion rod 403.
The detection device body 300 includes: a solution introducing zone, a solution transferring zone and a solution recovering zone.
Fig. 7 and 8 schematically show the structure of the solution introduction area of the detection device body 300. A solution introduction area is provided at the left side of the sample device body 300, the solution introduction area including: a sample tube guiding space 301, a puncture member 302, and a solution introducing port 303; the sample tube guiding space 301 is used to provide guiding and positioning for the sample collection tube 200. The piercing member 302 is disposed at the bottom of the sample tube guiding space 301 for piercing the lower end 2021 of the collection tube body 202. The solution inlet 303 is used to introduce the solution flowing out of the sample collection tube 200.
The lower end 2021 and the protruding sidewall 2022 of the lower portion of the collection tube 202 are adapted to the lower structure of the solution introduction area of the detection device body 300: the protruding sidewall 2022 cooperates with the puncture member 302 and the solution inlet 303 to prevent the solution from being contaminated by the outside air.
It should be noted that: in order to prevent the solution from falling from the periphery of the piercing member 302, fig. 8 schematically shows that a solution guide pipe is provided at one side of the piercing member 302, the solution guide pipe being in communication with the solution introduction port 303.
Fig. 2, 9 and 10 schematically show the structure of the solution transfer region of the detection device body 300. The solution transfer zone comprises: a first bottom channel 304, a first vertical channel 305, a middle displacement space 306, a second vertical channel 307, a piston vertical displacement space 308, a third vertical channel 309, an outflow channel 3010, a fluid channel 3011.
The solution inlet 303 is communicated with the first bottom channel 304, and the first bottom channel 304 is communicated with the lower port of the first vertical channel 305; the upper port of the first vertical channel 305 is communicated with the middle moving space 306; the lower ports of the second vertical channel 307 and the third vertical channel 309 are communicated with the middle moving space 306; the upper port of the second vertical channel 307 is communicated with the lower port of the piston vertical movement space 308;
one end of the outflow passage 3010 communicates with the upper port of the third vertical passage 309, and the other end of the outflow passage 3010 communicates with the inlet rod connection hole 3014.
The quantitative transfer of the sample solution is: the solution transfer area of the detection device body 300, the piston control rod 500 and the liquid adjusting moving member 600 are matched together.
Fig. 11 to 13 schematically show the structure of the liquid adjusting moving member 600. The liquid adjusting moving member 600 includes: a constant volume liquid storage space 601, an air compression space 602, and a horizontal projection 603. The quantitative liquid storage space 601 is communicated up and down; the air compression space 602 is an L-shaped space that includes two openings: upper and horizontal ports provided on the horizontal projection 603.
Correspondingly, the fluid channel 3011 is a wall groove of the middle moving space 306, and comprises a longitudinal horizontal section 3011-1, a vertical section 3011-2 and a transverse horizontal section 3011-3, wherein the directions of the longitudinal horizontal section 3011-1 are mutually perpendicular. The transverse horizontal section 3011-3 is at the lower portion of the longitudinal horizontal section 3011-1; that is, the wall portion groove, the horizontal protrusion 603 and the liquid adjusting moving member 600 together form a fluid passage 3011 (i.e., the liquid adjusting moving member 600 is moved rightward by a certain distance, the fluid passage forms a closed space with only 2 ports, and one side is not closed during the operation).
It should be noted that the fluid channel may be a channel disposed inside the middle moving space 306, that is, the horizontal protrusion 603 does not have to be a sealing surface of the fluid channel 3011.
Solution transfer includes two steps:
action one: as shown in fig. 14, the liquid adjusting moving member 600 is inserted into the middle moving space 306 in advance, the upper port of the first vertical channel 305 is communicated with the lower port of the quantitative liquid storing space 601, the shape and the size of the upper port of the quantitative liquid storing space 601 are adapted, and the upper port of the quantitative liquid storing space 307 is communicated with the lower port of the second vertical channel 307, and the shape and the size of the upper port of the quantitative liquid storing space are adapted;
the piston control rod 500 is previously disposed in the piston vertical movement space 308;
when the piston control rod 500 is pulled upward, the flow direction of the solution is: sample is taken from sample collection tube 200-solution inlet 303-first bottom channel 304-first vertical channel 305-quantitative reservoir space 601-second vertical channel 307-piston vertical movement space 308.
Action two: as shown in fig. 15, the liquid adjusting moving member 600 is moved rightward by a distance, at this time, the upper port of the quantitative liquid storing space 601 is aligned with the lower port of the third vertical channel 309, and correspondingly, the upper port of the air compressing space 602 is aligned with the lower port of the second vertical channel 307; that is, the distance between the lower port of the second vertical passage 307 and the lower port of the third vertical passage 309 is L, and the distance between the upper port of the quantitative liquid storage space 601 and the upper port of the air compression space 602 is M, l=m.
At the same time, the horizontal ports of the air compression space 602 are also aligned with the fluid passage 3011, and the ports of the lateral horizontal sections 3011-3 (i.e., the lower ports of the fluid passage 3011) are aligned with the lower ports of the volume reservoir space 601.
When the piston control rod 500 is pushed downward, the air in the air compression space 602 is compressed, and then enters the inside of the fluid channel 3011 through the horizontal port of the air compression space 602, then enters the lower port of the quantitative liquid storage space 601 through the lower port of the fluid channel 3011, and the solution in the quantitative liquid storage space 601 is extruded into the third vertical channel 309 through the compressed air, then sequentially extruded into the outflow channel 3010, and finally enters the reaction tube 400. After the solution enters the reaction tube 400, a heating reaction is performed.
When the piston control rod 500 is pushed downward, the flow direction of the gas is: an air compression space 602-a fluid passage 3011-a quantitative liquid storage space 601; the flow direction of the solution is: the quantitative liquid storage space 601, the third vertical channel 309, the outflow channel 3010, the liquid inlet of the liquid inlet pipe 404, the liquid storage space 405, the liquid outlet pipe 406 and the liquid outlet insertion rod 403 of the liquid insertion rod 402.
Fig. 16 is a diagram schematically showing the connection relationship between the reaction tube 400 and the detection device body 300. On the right side of the detection device body 300, from top to bottom, there are sequentially provided: the liquid outlet insertion rod connection hole 3012, the auxiliary insertion rod connection hole 3013, and the liquid inlet insertion rod connection hole 3014, and the liquid outlet insertion rod 403, the auxiliary insertion rod 401, and the liquid inlet insertion rod 402 are sequentially inserted into the liquid outlet insertion rod connection hole 3012, the auxiliary insertion rod connection hole 3013, and the liquid inlet insertion rod connection hole 3014.
As can be seen from fig. 9: the upper right side of the detecting device body 300 is provided with a solution recovery area 3015, and the solution outlet insertion rod connection hole 3012 is provided with a through hole, so that the solution recovery area 3015 is communicated with the solution outlet of the solution outlet insertion rod when the reaction tube 400 is inserted into the detecting device body 300. Above the solution recovery zone 3015 is provided a ventilation opening provided with a filter cartridge 700.
< description of quantitative transfer >
The present application is to achieve quantitative transfer by adjusting the distance of the upward and downward movement of the piston control rod 500. The design factors include:
A. the reaction tube must be inserted horizontally.
B. The reaction tube is only filled with lyophilized powder or lyophilized pellet.
On the basis of the above, kinetics were studied.
The solution in the metered dose holding space 601 moves upward from the third vertical channel 309 into the outflow channel 3010, which outflow channel 3010 is horizontal (which may be slightly sloped toward the feed insert rod connection aperture 3014 to facilitate solution flow).
Since the third vertical channel 309 and the quantitative liquid storage space 601 are vertically communicated channels (the diameters are the same, that is, the third vertical channel 309 and the quantitative liquid storage space 601 are all cylindrical).
The pressure of the air in the lower part of the solution is greater than the critical pressure P 1 When the solution is jacked up, critical pressure P 1 The method comprises the following steps:
P 1 =P 0 +ρgh；
wherein P is 0 Representing standard atmospheric pressure, ρ representing the density of the solution, g being the gravitational acceleration, h representing the height of the quantitative liquid storage space 601;
at the same time, P 1 It can also be expressed as:
P 1 = P 0 V 1 /V 2 ；
V 1 is the sum of the volume of the fluid passage 3011 and the volume of the air compression space 602.
V 2 Representing the volume of compressed air in the original fluid passage 3011 and air compression space 602.
The second vertical channel 307, the air compression space 602, the fluid channel 3011, the third vertical channel 309 and the quantitative liquid storage space 601 all adopt pipelines with the same diameter, and the radius thereof adopts r 1 And (3) representing. The radius of the piston vertical movement space 308 adopts r 2 And (3) representing.
The distance the piston control rod 500 is pulled upward is x and the distance the solution rises to the piston vertical movement space 308 is y.
In order to make the solution amount in the vertical movement space of the piston meet the requirement of quantitative pipetting (since the maximum volume of liquid transfer is the volume of the quantitative pipetting space 601), the requirement is that:
πr 2 2 y≥（V 1 -V 2 ）+V 3 =[V 1 - P 0 V 1 /（P 0 +ρgh）]+ V 3 =V 1 ρgh /（P 0 +ρgh）+ V 3 。
wherein V is 3 Indicating quantitative storageThe volume of the liquid space 601 plus the volume of the third vertical channel 309.
The symbols used in the present application and the physical quantity relation:
P 0 -standard atmospheric pressure.
P 1 The critical pressure at which the solution in the holding space 601 moves upwards is quantified.
ρ -density of solution.
g-gravitational acceleration.
h-quantifying the height of the reservoir 601.
V 1 The sum of the volume of the fluid passage 3011 and the volume of the air compression space 602.
V 2 The volume of compressed air of the original fluid passage 3011 and the air compression space 602 is P 0 V 1 /（P 0 +ρgh）。
V 3 The sum of the volume of the holding space 601 and the volume of the third vertical channel 309 is quantified.
r 1 The pipe radius of the second vertical channel 307, the air compression space 602, the fluid channel 3011, the third vertical channel 309, the dosing reservoir 601.
r 2 Radius of the piston vertical movement space 308.
The distance the x-piston control rod 500 is pulled upward.
When the y-piston control rod 500 is pulled upward, the solution rises to the distance of the piston vertical movement space 308.
The above examples are provided for convenience of description of the present application and are not to be construed as limiting the application in any way, and any person skilled in the art will make partial changes or modifications to the application by using the disclosed technical content without departing from the technical features of the application.
Claims (9)
1. A pull type nucleic acid detection quantitative transfer consumable, comprising: the device comprises a detection device body, a reaction tube, a piston control rod and a liquid adjusting moving component;
wherein, transfer liquid moving member includes: the quantitative liquid storage space and the air compression space are respectively provided with ports on the upper surface and the lower surface of the liquid adjusting moving component; the air compression space is provided with ports on the upper surface and one side surface of the liquid adjusting moving component, which are respectively called an upper port of the air compression space and a horizontal port of the air compression space;
wherein, detection device body includes: a solution introduction zone, a solution transfer zone;
the solution introduction zone comprises: a piercing member, a solution introduction port; the puncture member is used for puncturing the lower end part of the collecting pipe body; the solution inlet is used for introducing the solution flowing out of the sample collection tube;
the solution transfer zone comprises: the middle part moves the space, the piston moves the space vertically, the third vertical channel, the outflow channel, the fluid channel; the solution guide inlet is communicated with the lower surface of the middle moving space through a diversion quantitative liquid storage pipeline;
the piston vertical movement space is communicated with the upper surface of the middle movement space; the lower port of the third vertical channel is communicated with the middle moving space; one end of the outflow channel is communicated with the upper port of the third vertical channel, and the other end of the outflow channel is communicated with the reaction tube; the fluid channel is arranged on the side wall of the middle moving space, ports are arranged on the side surface and the lower surface of the middle moving space, and the fluid channel is divided into a horizontal port called the fluid channel and a lower port called the fluid channel;
the piston control rod can move up and down in the vertical movement space of the piston;
wherein the liquid adjusting moving member can move along the middle moving space;
in an initial state, the lower port of the quantitative liquid storage space is communicated with the upper port of the diversion quantitative liquid storage pipeline, and the upper port of the quantitative liquid storage space is communicated with the vertical movement space of the piston;
after the liquid adjusting moving member moves for a certain distance, the lower port of the quantitative liquid storage space can be communicated with the lower port of the fluid channel, and the upper port of the quantitative liquid storage space can be communicated with the lower port of the third vertical channel; the upper port of the air compression space is communicated with the vertical movement space of the piston, and the horizontal port of the air compression space is communicated with the horizontal port of the fluid channel;
the vertical movement space of the piston is cylindrical;
y、r 2 、h、V 1、 V 3 the requirements are as follows: pi r 2 2 y≥V 1 ρgh /（P 0 +ρgh）+ V 3 ；
Wherein r is 2 Representing the radius of the vertical movement space of the piston;
wherein y represents the distance from the solution to the vertical movement space of the piston when the piston control rod is pulled upwards;
wherein V is 1 Representing the sum of the volume of the fluid passage and the volume of the air compression space;
wherein ρ represents the density of the solution;
wherein g represents gravitational acceleration;
wherein h represents the height of the quantitative liquid storage space;
wherein P is 0 Represents 1 standard atmospheric pressure;
wherein V is 3 Representing the sum of the volume of the quantitative liquid storage space and the volume of the third vertical channel.
2. The pull type nucleic acid detecting quantitative transfer consumable according to claim 1, wherein the reaction tube comprises: auxiliary insertion rod, liquid inlet insertion rod, liquid outlet insertion rod, liquid inlet pipeline, liquid storage space and liquid outlet pipeline; the liquid outlet insertion rod, the auxiliary insertion rod and the liquid inlet insertion rod are sequentially arranged in parallel from top to bottom; the liquid inlet insertion rod is provided with a liquid inlet which is communicated with the liquid inlet pipeline; the liquid outlet inserting rod is provided with a liquid outlet which is communicated with the liquid outlet pipeline; the liquid inlet pipeline is communicated with the lower end of the liquid storage space, and the liquid outlet pipeline is communicated with the upper end of the liquid storage space.
3. The pull type nucleic acid detecting quantitative transferring consumable according to claim 2, wherein the pull type nucleic acid detecting quantitative transferring consumable further comprises: a filter element and a solution recovery zone; a solution recovery area is arranged on the detection device body; a ventilation port is arranged above the solution recovery area, and a filter element is arranged at the ventilation port;
the liquid outlet inserting rod connecting hole is provided with a through hole, so that when the reaction tube is inserted into the detection device body, the solution recovery area is communicated with the liquid outlet of the liquid outlet inserting rod.
4. The pull-out quantitative transfer consumable for nucleic acid detection according to claim 3, wherein the right side of the detection device body is provided with: go out liquid insert pole connecting hole, supplementary insert pole connecting hole, feed liquor insert pole connecting hole, go out liquid insert pole supplementary insert pole feed liquor insert pole insert in proper order go out liquid insert pole connecting hole, supplementary insert pole connecting hole, feed liquor insert pole connecting hole.
5. The pull type nucleic acid detecting quantitative transferring consumable according to claim 1, wherein the pull type nucleic acid detecting quantitative transferring consumable further comprises: a sample collection tube; the sample acquisition tube includes: a collecting tube body; the lower part of collection pipe body includes: a lower end portion and a protruding side wall portion protruding from the lower end portion.
6. The drawing type nucleic acid detection quantitative transfer consumable according to claim 4, wherein the diversion quantitative liquid storage pipeline consists of a first bottom channel and a first vertical channel, the solution inlet is communicated with the lower surface of the middle moving space through the first bottom channel and the first vertical channel, the solution inlet is communicated with the first bottom channel, and the first bottom channel is communicated with the lower port of the first vertical channel; the upper port of the first vertical channel is communicated with the middle moving space.
7. The pull type nucleic acid detecting and quantitatively transferring consumable according to claim 6, wherein the piston vertical moving space is communicated with the upper surface of the middle moving space through a second vertical channel, a lower port of the second vertical channel is communicated with the middle moving space, and an upper port of the second vertical channel is communicated with a lower port of the piston vertical moving space.
8. The pull type nucleic acid detecting quantitative transferring consumable according to claim 7, wherein the air compression space is an L-shaped space.
9. The pull type nucleic acid detecting and quantitatively transferring consumable according to claim 8, wherein the pull type nucleic acid detecting and quantitatively transferring consumable comprises the following steps when transferring a solution:
step1: placing a sample collection tube into a solution introduction zone of a testing device body;
step2: the piston control rod is pulled upwards, and the flow direction of the solution is as follows: sample collection tube-solution introduction port-first bottom channel-first vertical channel-quantitative liquid storage space-second vertical channel-piston vertical movement space;
step3: moving the liquid adjusting moving member for a distance to align the upper port of the quantitative liquid storage space with the lower port of the third vertical channel, and align the lower port of the quantitative liquid storage space with the lower port of the fluid channel; meanwhile, the upper port of the air compression space is aligned with the lower port of the second vertical channel, and the horizontal port of the air compression space is communicated with the horizontal port of the fluid channel;
step4: the piston control rod pushes downwards:
the flow direction of the gas is: an air compression space-a fluid channel-a quantitative liquid storage space-a third vertical channel;
the flow direction of the solution is: quantitative liquid storage space, third vertical channel, outflow channel, liquid inlet of liquid insertion rod, liquid inlet pipeline, liquid storage space, liquid outlet pipeline and liquid outlet insertion rod.
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| CN208505683U (en) * | 2018-07-19 | 2019-02-15 | 厦门华厦学院 | A kind of laboratory sampling Absorption quantity device |
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| CN112877192A (en) * | 2021-04-09 | 2021-06-01 | 广州市达安医疗器械有限公司 | Closed nucleic acid detection consumable and detection method |
| CN113278512A (en) * | 2021-05-31 | 2021-08-20 | 广州源起健康科技有限公司 | Integrated sample treatment consumable for automatic nucleic acid detection |
| CN116121039A (en) * | 2021-11-13 | 2023-05-16 | 圣湘生物科技股份有限公司 | Sample detection consumable and sample detection method |
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|---|---|---|---|---|
| CN208505683U (en) * | 2018-07-19 | 2019-02-15 | 厦门华厦学院 | A kind of laboratory sampling Absorption quantity device |
| CN112665918A (en) * | 2019-10-15 | 2021-04-16 | 中国石油化工股份有限公司 | Closed sampling and returning device for flowing medium |
| CN112877192A (en) * | 2021-04-09 | 2021-06-01 | 广州市达安医疗器械有限公司 | Closed nucleic acid detection consumable and detection method |
| CN113278512A (en) * | 2021-05-31 | 2021-08-20 | 广州源起健康科技有限公司 | Integrated sample treatment consumable for automatic nucleic acid detection |
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Effective date of registration: 20231115 Address after: 221000 building 17, 19 and 22 of new micro semiconductor accelerator in Xuzhou Economic and Technological Development Zone, Jiangsu Province Patentee after: Jiangsu xunrui Biotechnology Co.,Ltd. Address before: 215213 rooms 101 and 201, C7 building, biomedical industrial park, 218 Xinghu street, Suzhou Industrial Park, Jiangsu Province Patentee before: Suzhou Yarui Biotechnology Co.,Ltd. |