CN117264748B - Reagent card assembly, incubator assembly, chromatographic kit and detection method - Google Patents
Reagent card assembly, incubator assembly, chromatographic kit and detection method Download PDFInfo
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- CN117264748B CN117264748B CN202311555253.0A CN202311555253A CN117264748B CN 117264748 B CN117264748 B CN 117264748B CN 202311555253 A CN202311555253 A CN 202311555253A CN 117264748 B CN117264748 B CN 117264748B
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 107
- 238000001514 detection method Methods 0.000 title abstract description 13
- 239000003085 diluting agent Substances 0.000 claims abstract description 137
- 238000003780 insertion Methods 0.000 claims abstract description 56
- 230000037431 insertion Effects 0.000 claims abstract description 56
- 239000007788 liquid Substances 0.000 claims abstract description 45
- 238000012360 testing method Methods 0.000 claims abstract description 40
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 13
- 238000003745 diagnosis Methods 0.000 claims abstract description 6
- 241000463219 Epitheca Species 0.000 claims abstract description 3
- 238000013461 design Methods 0.000 claims description 33
- 238000010438 heat treatment Methods 0.000 claims description 25
- 230000007246 mechanism Effects 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 11
- 239000002184 metal Substances 0.000 claims description 10
- 230000009194 climbing Effects 0.000 claims description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 239000000741 silica gel Substances 0.000 claims description 5
- 229910002027 silica gel Inorganic materials 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 4
- 238000011901 isothermal amplification Methods 0.000 claims description 3
- 238000005192 partition Methods 0.000 claims description 3
- 238000007689 inspection Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 29
- 238000010790 dilution Methods 0.000 description 8
- 239000012895 dilution Substances 0.000 description 8
- 229920001296 polysiloxane Polymers 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000010586 diagram Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 230000003028 elevating effect Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/126—Paper
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Plasma & Fusion (AREA)
- Biophysics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a reagent card assembly, an incubator assembly, a chromatography kit and a detection method, belonging to the fields of medical examination and inspection instruments and molecular diagnosis and detection instruments; the technical key points are as follows: a, the upper surface of reagent card epitheca is provided with: n observation windows and liquid inlet components; the liquid inlet assembly is provided with N liquid inlets; b, the reagent card base includes: n reaction test channels; the N reaction test strips, the N reaction test channels and the N observation windows are arranged in one-to-one correspondence; c, the lower surface of reagent card base still is provided with: a second insertion space; the second insertion space is used for inserting the diluent bin; d, the reagent card base still includes: n diluent connecting channels and N puncture tubes; one end of the diluent connecting channel is communicated with the sample adding reaction bin, and the other end of the diluent connecting channel is connected with a puncture tube; the puncture tube is used for being connected with the diluent bin.
Description
Technical Field
The invention relates to the field of medical examination and inspection instruments and molecular diagnosis and detection instruments, in particular to a reagent card assembly, an incubator assembly, a chromatographic kit and a detection method.
Background
CN115141745B discloses a multi-channel split type kit, a nucleic acid detecting device and a detecting method. The working mode is as follows:
s1, unscrewing a sealing cover of a sample adding port, and adding a sample from the sample adding port; a dilution pipe for storing dilution liquid is arranged on the sample adding port;
s2, a sample flows into the reaction bin along the flow channel, and diluent is stored in the diluent pipe due to liquid tension;
s3, turning on a power switch of the base matrix to heat and react, extruding a diluent preservation pipe after the reaction is finished, and enabling diluent to flow into a reaction bin along a flow channel;
s4, the mixed liquid in the reaction bin contacts with a test strip along a flow channel, and chromatographic reaction shows a result.
The working modes can be known from the above working modes: the chromatographic kit disclosed in CN115141745B requires uncapping in use, and then manually squeezing the diluent tube to squeeze the diluent into the reaction cartridge, which can create several problems:
1) After the sampling tube is installed, the dilution tube is detached and installed again; contamination may result in the process.
2) The chromatographic kit is used as a user self-test, and the operation of the chromatographic kit is necessarily inexperienced. When the CN115141745B is used, a user is required to extrude the dilution pipe by himself, and when the dilution pipe is used, different people, different extrusion modes and different forces have great influence on the test result. Therefore, the above operation causes both inconvenience to the user and an influence on the measurement accuracy.
Based on the above technical requirements, development of chromatographic kits is required to be continued.
Disclosure of Invention
The present invention aims to address the above-mentioned shortcomings of the prior art by providing a reagent card assembly.
It is another object of the present application to provide an incubator assembly.
It is yet another object of the present application to provide a chromatography kit.
It is still another object of the present application to provide a detection method.
The technical scheme of the invention is as follows:
a reagent card assembly, comprising: a reagent card upper shell, a reagent card base and N reaction test strips; n is a natural number greater than or equal to 1;
a, the upper surface of reagent card epitheca is provided with: n observation windows and liquid inlet components; the liquid inlet assembly is provided with N liquid inlets;
b, the reagent card base includes: the N reaction test channels, the N reaction test strips, the N reaction test channels and the N observation windows are arranged in a one-to-one correspondence manner;
c, the lower surface of reagent card base still is provided with: a second insertion space; the second insertion space is used for inserting the diluent bin;
d, the reagent card base still includes: n diluent connecting channels and N puncture tubes; one end of the diluent connecting channel is communicated with the sample adding reaction bin, and the other end of the diluent connecting channel is connected with a puncture tube; the puncture tube is used for being connected with the diluent bin.
Further, the diluent connecting channel adopts a Z-shaped design.
Further, the reaction test channel sequentially includes, according to a flow direction of the solution: the sample adding reaction bin, the first horizontal channel, the inclined plane channel, the second horizontal channel and the supporting component; according to the flowing direction of the solution, the inclined surface channel is gradually increased; the reaction test strip is arranged on the inclined plane channel, the second horizontal channel and the supporting component.
An incubator assembly, comprising: comprising the following steps: the incubator comprises an incubator base, a horizontal telescopic rod assembly, a jacking mechanism assembly, a vibration motor, a heating block and a diluent bin pushing member;
wherein, horizontal telescopic link subassembly includes: the horizontal telescopic rod is used for fixing the metal plate, the horizontal telescopic rod is used for moving the rod and the horizontal moving block; the horizontal telescopic rod fixing metal plate is fixedly arranged on the incubator base; the fixed end of the movable rod of the horizontal telescopic rod is fixedly connected with the fixed metal plate of the horizontal telescopic rod, and the movable end of the movable rod is connected with the horizontal moving block;
wherein, climbing mechanism subassembly includes: the device comprises a bottom support column, a first fixed platform, a second vertical support column, a second lifting platform, a vertical jacking component, a horizontal driving component and a vertical spring component; the first fixed platform is arranged above the incubator base, and a bottom support column is arranged between the first fixed platform and the incubator base; a second vertical supporting column is fixedly arranged on the upper surface of the first fixed platform, a vertical guide hole is formed in the second lifting platform, and the second vertical supporting column penetrates through the vertical guide hole; the upper part of the second lifting platform is fixed with a vertical spring assembly; the vertical jacking component is fixed with the second lifting platform;
wherein, the cooperation relation of horizontal telescopic link subassembly and climbing mechanism subassembly is: the horizontal driving component and the horizontal moving block are fixedly connected into a whole; the horizontal driving member is horizontally movable on the first fixed platform; the contact surfaces of the horizontal driving member and the vertical jacking member are inclined planes, and when the horizontal driving member moves horizontally, the vertical jacking member can move vertically;
the vibration motor is arranged on the upper surface of the second lifting platform;
wherein the top of the vertical spring assembly is fixedly provided with the heating block;
wherein, diluent storehouse pushing component fixed setting is in the upper portion of horizontal migration piece.
Further, the horizontal telescopic rod assembly further comprises: the device comprises a first guide column fixing seat, a horizontal guide rod and a second guide column fixing seat; the first guide post fixing seat and the second guide post fixing seat are fixedly arranged on the incubator base; the horizontal guide rod is fixedly arranged between the first guide column fixing seat and the second guide column fixing seat; the horizontal moving block is provided with a guide hole, and the horizontal guide rod passes through the guide hole; the direction of the horizontal guide rod is parallel to the direction of the movable rod of the horizontal telescopic rod.
Further, the incubator assembly further comprises: an incubator assembly housing; the incubator assembly housing comprises: a first space, a second space, and a horizontal partition plate; the incubator base is adapted to the bottom of the incubator assembly enclosure; the horizontal separation plate is also provided with a strip-shaped hole, and the diluent bin pushing member passes through the strip-shaped hole;
in the initial state, the horizontal telescopic rod assembly, the jacking mechanism assembly, the vibration motor and the heating block are all arranged in the second space;
the bottom of the incubator assembly housing is adapted to the incubator base.
Further, the incubator assembly further comprises: a vertical button assembly and a horizontal ejection assembly;
wherein, vertical button subassembly includes: the first guide post is connected with the first pressing component; the pressing buckle plate is horizontally arranged, and the first spring, the second guide column and the second pressing member are vertically arranged;
the upper surface of the fixer is fixed with the lower end of the second guide post; the lower end of the second pressing component is provided with a groove extending into the second pressing component; the lower end of the first spring is fixed with the pressing buckle plate, and the upper end of the first spring is fixed with the lower surface of the fixer; the upper ends of the second springs and the second guide posts are inserted into grooves formed in the lower ends of the second pressing members;
wherein, the horizontal ejection assembly includes: a top cap pressing plate, an ejection spring and a top cap; the top cap pressing plate is a vertical plate, and the top cap and the ejection spring are horizontally arranged; one end of the ejection spring is fixed with the top cap pressing plate, and the other end of the ejection spring is fixed with the top cap; the top cap is provided with a concave space, and the ejection spring extends into the concave space;
the top cap pressing plate and the pressing buckle plate are fixedly connected with the incubator assembly shell.
A chromatography kit, comprising: the reagent card assembly and the diluent bin;
wherein, the diluent storehouse includes: the device comprises a diluent bin upper seat, a diluent bin base, N piston silica gel valves, N springs and N puncture membranes;
the upper seat of the diluent bin comprises N storage bins and N insertion bins;
the lower end part of the insertion bin adopts a step part design;
the diluent storehouse base includes: an outer shell, wherein N diluent inserting pipes are arranged in the outer shell;
the upper base of the diluent bin is inserted into the base of the diluent bin: the insertion bin is inserted into a gap between the outer shell and the insertion tube, and the insertion tube is inserted into the insertion bin;
a piston silica gel valve is arranged at the end part of the insertion tube inserted into the insertion bin, and the puncture membrane is arranged at the outer side of the other end part; one end of the spring is fixed with the bottom of the outer shell, and the other end of the spring is fixed with the step part of the insertion bin;
wherein the diluent bin is horizontally inserted into a second insertion space of the reagent card assembly; the puncture tube can be inserted into the insertion tube.
Further, the chromatography kit further comprises: an incubator assembly; the reagent card assembly is placed on the first space of the incubator assembly.
Further, the reagent card base further comprises: the first sliding groove, the second sliding groove, the first concave space and the third concave space; the first concave space is used for placing a motor and a heating block; the third concave space is used for the movement of the liquid releasing bin pushing component; the first sliding groove is arranged on the outer side of the reagent card base; the inner side walls of the first concave space and the second insertion space are provided with second sliding grooves;
the reagent card base is also provided with a reagent card component fixing groove; the holder is insertable into the reagent card assembly holding slot;
the diluent bin base further comprises: an outer housing boss;
the incubator assembly housing further comprises: a gear plate and a protruding block; a protruding block is arranged on the side wall of the first space and is matched with the first sliding groove; the upper surface at horizontal division board still is provided with two vertical fender boards, the fender board with outer housing boss cooperation is used for spacing to the diluent storehouse.
Further, the reagent card assembly further comprises: a reagent card cover; the reagent card cover is used for sealing the liquid inlet assembly.
A detection method is a method for diagnosing non-disease, which uses the chromatography kit and comprises the following steps:
s100, a diluent bin is placed in a second insertion space in advance;
s200, aligning N adopting pipes to N liquid inlets of a liquid inlet assembly, and enabling samples to flow into a sample adding reaction bin for isothermal amplification reaction;
s300, after the reaction is finished, pressing the diluent bin reciprocally to enable diluent in the diluent bin to enter the sample adding reaction bin;
s400, the mixed liquid in the sample adding reaction bin contacts with a test strip along a reaction test channel, and chromatographic reaction shows a result.
A method of detection, which is a method of non-disease diagnosis as a target, comprising the steps of:
s100, a diluent bin is placed in a second insertion space in advance;
s200, the horizontal telescopic rod assembly moves, so that the jacking mechanism assembly drives the vibration motor and the heating block to descend, and the vibration motor and the heating block are lower than the horizontal separation plate;
s300, pressing the vertical button assembly, horizontally pushing the reagent card assembly into the incubator assembly, loosening the vertical button assembly after the reagent card assembly is in place, and fixing the reagent card assembly by the vertical button assembly;
s400, tearing off the reagent card cover, aligning N adopting pipes with N liquid inlets of the liquid inlet assembly, and allowing samples to flow into the sample adding reaction bin;
s500, the horizontal telescopic rod assembly moves, so that the jacking mechanism assembly drives the vibration motor and the heating block to ascend, and the heating block is attached to the vibration motor and the reagent card assembly;
s600, after the reaction is finished, the horizontal telescopic rod assembly reciprocates, and the diluent bin pushing component reciprocally presses the diluent bin, so that diluent in the diluent bin enters the sample adding reaction bin;
s700, the mixed liquid in the sample adding reaction bin contacts with a test strip along a reaction test channel, and chromatographic reaction shows a result.
The beneficial effects of this application lie in:
first, the basic concept of the present application is: in use, the prior art CN115141745B requires removing the sampling tube from the loading port and installing the dilution tube on the loading port. The design idea of the application is as follows: two sets of pipelines are designed for the reagent card component: one set of tubing is used for sampling (i.e., the inlet assembly 2102 on the upper surface of the reagent cartridge upper housing 2100), and the other set of tubing is a diluent connection path, and when shipped, the piercing tubing is in direct contact with the piercing membrane or the piercing tubing is inserted directly into the diluent cartridge. The design ensures that the diluent connecting channel and the sample adding port are not contacted with air any more when the chromatographic kit is used, thereby further improving the detection accuracy and avoiding the problem of secondary pollution.
Second, the second invention point of the present application is that: the detection mode of the reagent card component. A method of detection comprising the steps of:
s100, a diluent bin is placed in a second insertion space in advance;
s200, aligning N adopting pipes to N liquid inlets of a liquid inlet assembly, and enabling samples to flow into a sample adding reaction bin for isothermal amplification reaction;
s300, after the reaction is finished, pressing the diluent bin reciprocally to enable diluent in the diluent bin to enter the sample adding reaction bin;
s400, the mixed liquid in the sample adding reaction bin contacts with a test strip along a reaction test channel, and chromatographic reaction shows a result.
Third, a third inventive aspect of the present application resides in the development of a dedicated incubator assembly.
3.1, the diluent cartridge pushing member is used to effect pressing of the diluent cartridge, no longer requiring the user to manipulate the diluent.
3.2, through a device (namely a horizontal telescopic rod), the vertical movement of the vibration motor and the heating block is realized, and the horizontal movement of the diluent bin pushing component is realized.
Fourth, a fourth invention of the present application resides in: the design of the vertical button assembly and the horizontal ejection assembly. Through vertical button subassembly and horizontal ejecting subassembly, make things convenient for the installation and the change of reagent card subassembly.
Specifically, upon installation of the reagent card assembly:
a, first press vertical button assembly 3800: when the second pressing member 3807 is pressed, the second guide post 3806 is acted to drive the fixer 3804 and the first pressing member 3802 to press down together, and when the first pressing member 3802 contacts the pressing buckle 3801, the pressing down cannot be continued;
b, then pushing the reagent card assembly 2000 horizontally into the first space of the incubator assembly 3000, at which time the ejector spring 3902 is compressed;
c. releasing the vertical button assembly 3800, under the action of the first spring 3803, the fixator 3804, the second spring 3805, the second guide post 3806, the second pressing member 3807, etc. return upward to the initial position, and at this time, the fixator 3804 (which adopts an L-shaped structure) is inserted into the reagent card assembly fixing groove 2214, thereby achieving the fixation of the reagent card assembly 2000;
in removing the reagent card assembly 2000, the following steps are taken:
a. pressing vertical button assembly 3800, retainer 3804 moves downward, withdrawing from reagent card assembly retaining groove 2214;
b. the reagent card pack driving member 2000 is automatically pushed out by the ejector spring 3902 of the horizontal ejector assembly 3900.
Drawings
The invention is described in further detail below in connection with the embodiments in the drawings, but is not to be construed as limiting the invention in any way.
FIG. 1 is a schematic three-dimensional design of the full-automatic chromatographic kit of example 1.
FIG. 2 is a schematic three-dimensional design of the reagent card assembly of example 1.
FIG. 3 is a schematic diagram showing the design of the upper surface of the upper case of the reagent card of example 1.
FIG. 4 is a schematic diagram showing the design of the upper surface of the reagent card base of example 1.
FIG. 5 is a detailed design schematic of the reaction test channel of example 1.
FIG. 6 is a schematic of the three-dimensional design of the reagent card assembly, diluent cartridge of example 1, mounted together.
FIG. 7 is a schematic view of the lower surface design of the reagent card assembly of example 1.
FIG. 8 is a top view of the reagent card base of example 1.
Fig. 9 is a sectional view A-A of fig. 8.
FIG. 10 is a schematic cross-sectional design of the reagent card assembly and diluent cartridge of example 1.
FIG. 11 is a schematic of the three-dimensional design of the diluent cartridge of example 1.
FIG. 12 is a cross-sectional view of the diluent cartridge of example 1.
FIG. 13 is a schematic of the three-dimensional design of the diluent sump base of example 1.
FIG. 14 is a schematic of the three-dimensional design of the incubator assembly of example 1.
FIG. 15 is a schematic of the three-dimensional design of the incubator assembly enclosure of example 1.
FIG. 16 is a schematic of the design of the incubator assembly housing of example 1 with the diluent cartridge mated.
FIG. 17 is a schematic three-dimensional design of the internal components of the fully automated chromatographic kit of example 1.
FIG. 18 is a schematic of the three-dimensional design of the internal components of the incubator assembly of example 1.
Fig. 19 is a three-dimensional design schematic of the horizontal telescopic rod assembly of embodiment 1.
Fig. 20 is a three-dimensional design schematic diagram of the climbing mechanism assembly of embodiment 1.
The reference numerals are explained as follows:
full-automatic chromatography kit 1000;
reagent card assembly 2000, reagent card upper housing 2100, reagent card base 2200, reagent card cover 2300, and reaction test strip 2400;
a viewing window 2101, a liquid inlet assembly 2102;
reaction test channel 2201, sample loading reaction chamber 2202, first horizontal channel 2203, inclined surface channel 2204, second horizontal channel 2205, support member 2206, first sliding groove 2207, second sliding groove 2208, first concave space 2209, second insertion space 2210, third concave space 2211, diluent connection channel 2212, puncture tube 2213, reagent card assembly fixing groove 2214;
incubator assembly 3000, incubator base 3100, horizontal telescoping rod assembly 3200, climbing mechanism assembly 3300, vibration motor 3400, heating block 3500, diluent cartridge pushing member 3600, incubator assembly housing 3700;
horizontal telescopic rod fixing metal plate 3201, horizontal telescopic rod moving rod 3202, horizontal moving block 3203, first guide post fixing base 3204, horizontal guide rod 3205 and second guide post fixing base 3206;
bottom support columns 3301, first fixed platforms 3302, second vertical support columns 3303, second lifting platforms 3304, vertical jacking members 3305, horizontal drive members 3306, vertical spring assemblies 3307;
first space 3701, second space 3702, horizontal divider plate 3703, gear plate 3705, and projection 3706;
a diluent cartridge 4000, a diluent cartridge upper seat 4100, a diluent cartridge base 4200, a piston silicone valve 4300, a spring 4400, a seal 4500, and a piercing membrane 4600.
Detailed Description
The objects, technical solutions and advantages of the present invention will become more apparent by the following detailed description of the present invention with reference to the accompanying drawings. It should be understood that the description is only illustrative and is not intended to limit the scope of the invention. In addition, in the following description, descriptions of well-known structures and techniques are omitted so as not to unnecessarily obscure the present invention.
< example 1 > A full-automatic chromatography kit
Fig. 1 schematically shows the general construction of a full-automatic chromatography kit 1000. A fully automated chromatography kit 1000 comprising: reagent card assembly 2000, incubator assembly 3000, diluent cartridge 4000.
Fig. 2 and 3 illustrate the three-dimensional structural design of the reagent card assembly 2000 from different angles. A reagent card assembly 2000 comprising: reagent card upper case 2100, reagent card base 2200, reagent card cover 2300, and N reaction test strips 2400.
The upper surface of the reagent card upper case 2100 is provided with: n observation windows 2101, a liquid inlet assembly 2102; the liquid inlet assembly is provided with N liquid inlets;
the reagent card cap 2300 is used to seal the inlet assembly 2102.
In the present application, N is a natural number equal to or greater than 1, which represents the number of test channels of the chromatographic kit.
Fig. 4, 5, and 8 illustrate the structural design of the upper surface of the reagent card base 2200. The reagent card base 2200 includes: n reaction test channels 2201; the reaction test channel 2201 sequentially includes, in terms of the flow direction of the solution: a loading reaction chamber 2202, a first horizontal channel 2203, a bevel channel 2204, a second horizontal channel 2205, and a support member 2206; the inclined surface channel is gradually increased according to the flowing direction of the solution, that is, the height of the second horizontal channel 2205 is higher than the height of the first horizontal channel 2203; the reaction test strip 2400 is disposed on the inclined surface channel 2204, the second horizontal channel 2205, and the support member 2206; the end part of the reaction test strip corresponds to the observation window; n reaction test strips 2400 are arranged corresponding to N reaction test channels 2201.
Fig. 6 and 7 are schematic diagrams showing a design of the lower surface of the reagent card base 2200. Reagent card base 2200 further comprises: a first sliding groove 2207, a second sliding groove 2208, a first concave space 2209, a second insertion space 2210, a third concave space 2211; the first concave space 2209 is used for placing a motor 3400 and a heating block 3500; the second insertion space 2210 is used for inserting the diluent cabin 4000; the third concave space 2211 is used for placing the diluent chamber 4000 and the liquid releasing chamber pushing component 3600; the first sliding groove 2207 is provided on the outside of the reagent card base 2200; a second sliding groove 2208 is provided in a side wall of the first concave space 2209 and the second insertion space 2210.
Fig. 9 illustrates a cross-sectional view of the reagent card base 2200, and fig. 10 illustrates a cross-sectional view of the reagent card base 2200 mated with the diluent cartridge 4000. Reagent card base 2200 further comprises: n diluent connection passages 2212; the diluent connection 2212 may be of a Z-type design. One end of the diluent connecting channel 2212 is communicated with the sample adding reaction bin 2202, and the other end is connected with a puncture tube 2213.
Fig. 11 illustrates a schematic three-dimensional design of the diluent chamber 4000. Fig. 12 illustrates a schematic three-dimensional design of the diluent cartridge 4000. Fig. 13 shows schematically the structural design of the base of the diluent compartment. Dilution liquid bin 4000 the dilution liquid bin 4000 comprises: the diluent cartridge upper housing 4100, the diluent cartridge base 4200, the N piston silicone valves 4300, the N springs 4400, the N seal rings 4500, the N piercing membranes 4600.
Wherein the diluent cartridge upper seat 4100 comprises N storage cartridges 4101 and N insertion cartridges 4102; the lower end of the insertion bin 4102 adopts a step design;
wherein the diluent cartridge base 4200 comprises: an outer housing 4201, an outer housing boss 4202; n diluent pipes 4203 are provided inside the outer case 4201;
wherein the diluent cartridge upper seat 4100 is inserted into the diluent cartridge base 4200: the insertion cartridge 4102 is inserted into a space between the outer case 4201 and the insertion tube 4203, and the insertion tube 4203 is inserted into the insertion cartridge 4102;
wherein, a piston silica gel valve 4300 is mounted on the end of the insertion tube 4203 inserted into the insertion chamber 4102, and the puncture membrane 4600 is provided on the outer side of the other end;
wherein, the insertion tube 4203 protrudes from the bottom of the outer housing 4201, and a sealing ring 4500 is sleeved on the protruding portion;
wherein, one end of the spring 4400 is fixed with the bottom of the outer housing, and the other end is fixed with the step of the insertion bin 4102.
The matching process of the diluent chamber 4000 and the reagent card assembly 2000 is as follows:
step a, placing the diluent cabin 4000 in the first concave space 2209, and inserting the boss of the diluent cabin 4000 into the second sliding groove 2208;
step b, pushing the diluent cartridge 4000 from the first concave space 2209 to the second insertion space 2210;
step c, continuing to push the diluent cartridge 4000 so that the puncture tube 2213 is inserted into the corresponding insertion tube 4203 through the puncture membrane 4600;
step d, reciprocally extruding the diluent bin 4000:
when the upper seat 4100 of the diluent bin is pressed forwards, the diluent is extruded, and the specific solution flow direction is as follows: "storage bin 4101, piston silicone valve 4300, diluent insertion tube 4203, puncture tube 2213, diluent connection passage 2212" flows to reaction bin, diluent bin upper seat 4100 is released, at this time spring 4400 pushes back diluent bin upper seat 4100, and liquid flows back; and the process is repeated for several times, so that the liquid in the sample adding reaction bin is diluted, finally, the upper seat 4100 of the diluted liquid bin is completely pushed in, the mixed liquid in the sample adding reaction bin enters into the first horizontal channel 2203, contacts with the test strip through the flow channel, and the reaction color development result is obtained.
The working principle of the piston silicone valve 4300 is as follows: the interior of the piston silicone valve 4300 is hollow and is sleeved at the upper end of the insertion tube 4203; as shown in fig. 13, the top surface of the piston silicone valve 4300 is provided with a cutout (the shape of the cutout is preferably "+" shaped); when the diluent cartridge upper seat 4100 is not pressed, the cut is closed and the storage cartridge 4101 cannot flow to the diluent insertion tube 4203; upon squeezing the diluent cartridge upper seat 4100, the storage cartridge 4101 is punched with a cross-shaped cutout, flowing toward the diluent insertion tube 4203. That is, the piston silicone valve 4300 functions similarly to a check valve.
It should be noted that: sample solution is added to the sample adding port and enters the sample adding reaction chamber 2202, and the solution height is lower than the connection part of the diluent connecting channel 2212 and the sample adding reaction chamber 2202.
Fig. 14 illustrates a three-dimensional schematic of incubator assembly 3000. Fig. 15 illustrates a three-dimensional schematic of an incubator assembly housing 3700. Incubator assembly 3000, comprising: incubator base 3100, incubator assembly housing 3700; the incubator assembly housing 3700 includes: first space 3701, second space 3702, horizontal divider plate 3703, gear plate 3705, and projection 3706;
wherein the incubator base 3100 mates with the bottom of the incubator assembly housing 3700;
wherein a bar-shaped hole 3704 is further provided on the horizontal partition plate 3703, and a diluent cartridge pushing member 3600 passes through the bar-shaped hole 3704;
wherein a protrusion 3706 is provided on a sidewall of the first space 3701, which is fitted with the first sliding groove 2207.
As shown in fig. 16, two vertical baffles 3705 are also provided on the upper surface of the horizontal divider plate 3704 for limiting the diluent chamber 4000 (the baffles 3705 correspondingly mate with the outer housing boss 4202).
Fig. 17 schematically illustrates the internal structure of the incubator assembly 3000. Fig. 18 schematically illustrates the internal structure of the incubator assembly 3000. Incubator assembly 3000, comprising: horizontal telescopic rod assembly 3200, climbing mechanism assembly 3300, vibration motor 3400, heating block 3500, diluent cartridge pushing member 3600.
Fig. 19 illustrates a three-dimensional structure of horizontal telescopic rod assembly 3200. Horizontal telescopic rod assembly 3200 includes: horizontal telescopic rod fixing metal plate 3201, horizontal telescopic rod moving rod 3202, horizontal moving block 3203, first guide post fixing base 3204, horizontal guide rod 3205 and second guide post fixing base 3206;
wherein the horizontal telescopic rod fixing metal plate 3201, the first guide post fixing base 3204 and the second guide post fixing base 3206 are fixedly arranged on the incubator base 3100;
wherein, the fixed end of the movable rod 3202 of the horizontal telescopic rod is fixedly connected with the fixed metal plate 3201 of the horizontal telescopic rod, and the movable end of the movable rod is connected with the horizontal moving block 3203;
wherein, the horizontal guide rod 3205 is fixedly arranged between the first guide post fixing seat 3204 and the second guide post fixing seat 3206;
wherein, a guide hole is provided on the horizontal moving block 3203, and the horizontal guide bar 3205 passes through the guide hole; the direction of the horizontal guiding rod 3205 is parallel to the direction of the movable rod 3202 of the horizontal telescopic rod.
Fig. 20 illustrates a three-dimensional structure of a climbing mechanism assembly 3300. Climbing mechanism assembly 3300, including: bottom support columns 3301, first fixed platforms 3302, second vertical support columns 3303, second lifting platforms 3304, vertical jacking members 3305, horizontal drive members 3306, vertical spring assemblies 3307;
wherein the first stationary platform 3302 is above the incubator base 3100, a bottom support column 3301 is provided between the first stationary platform 3302 and the incubator base 3100;
wherein, the horizontal driving member 3306 is fixedly connected with the horizontal moving block 3203 into a whole;
wherein the horizontal driving member 3306 is disposed at an upper side of the first fixed platform 3302, and the horizontal driving member 3306 can horizontally move on the first fixed platform 3302;
wherein, the contact surfaces of the horizontal driving member 3306 and the vertical jacking member 3305 are inclined surfaces; the vertical elevating member 3305 can move up/down while the horizontal driving member 3306 moves horizontally;
wherein the vertical lifting member 3305 is connected with the second lifting platform 3304.
In addition, it should be noted that: a vibration motor 3400 is provided on an upper surface of the second elevating platform 3304.
In addition, it should be noted that: a vertical spring assembly 3307 is fixed on the upper portion of the second lifting platform 3304, and the heating block 3500 is fixedly arranged on the top of the vertical spring assembly 3307.
In addition, it should be noted that: the diluent cartridge pushing member 3600 is fixedly provided at an upper portion of the horizontal moving block 3203.
As shown in fig. 18 and 20, the incubator assembly 3000 further includes: vertical button assembly 3800, horizontal ejection assembly 3900.
The vertical button assembly 3800 includes: pressing buckle 3801, first pressing member 3802 (serving as a stop), first spring 3803, anchor 3804, second spring 3805, second guide post 3806, second pressing member 3807; the pressing buckle 3801 is horizontally disposed, and the first pressing member 3802, the first spring 3803, the second spring 3805, the second guide post 3806, and the second pressing member 3807 are all vertically disposed;
the upper end of the first pressing member 3802 is fixed to the lower surface of the holder 3804, and the upper surface of the holder 3804 is fixed to the lower end of the second guide post 3806;
the lower end of the second pressing member 3807 is provided with a groove extending into the inside;
the first spring 3803 is wound around the first pressing member 3802, the lower end of the first spring 3803 is fixed to the pressing buckle 3801, and the upper end thereof is fixed to the lower surface of the holder 3804;
in the initial state, the lower end of the first pressing member 3802 is spaced from the pressing buckle 3801, and the second spring 3805 and the upper end of the second guide post 3806 are inserted into a groove provided at the lower end of the second pressing member 3807 (the second spring 3805, the upper end of the second guide post 3806 and the second pressing member 3807 may or may not be fixedly connected).
The horizontal ejection assembly 3900 includes: top cap platen 3901, top cap 3903, and top spring 3902; the top cap pressing plate 3901 is a vertical plate, and the top cap 3903 and the ejection spring 3902 are horizontally arranged; one end of the ejection spring 3902 is fixed to the top cap pressing plate 3901, and the other end is fixed to the top cap 3903. The top cap is provided with a concave space into which the ejection spring 3902 protrudes.
It should be noted that: the top cap platen 3901 and the press pinch plate 3801 are fixedly connected to the incubator assembly housing 3700.
The mating design of vertical button assembly 3800, horizontal ejection assembly 3900 is:
when the incubator assembly 3000 is initially installed with the reagent card assembly 2000, the following steps are adopted:
a, first press vertical button assembly 3800: when the second pressing member 3807 is pressed, the second guide post 3806 is acted to drive the fixer 3804 and the first pressing member 3802 to press down together, and when the first pressing member 3802 contacts the pressing buckle 3801, the pressing down cannot be continued;
b, then pushing the reagent card assembly 2000 horizontally into the first space of the incubator assembly 3000, at which time the ejector spring 3902 is compressed;
c. releasing the vertical button assembly 3800, under the action of the first spring 3803, the fixator 3804, the second spring 3805, the second guide post 3806, the second pressing member 3807, etc. return upward to the initial position, and at this time, the fixator 3804 (which adopts an L-shaped structure) is inserted into the reagent card assembly fixing groove 2214, thereby achieving the fixation of the reagent card assembly 2000;
when the incubator assembly 3000 removes the reagent card assembly 2000, the following steps are adopted:
a. pressing vertical button assembly 3800, retainer 3804 moves downward, withdrawing from reagent card assembly retaining groove 2214;
b. the reagent card pack driving member 2000 is automatically pushed out by the ejector spring 3902 of the horizontal ejector assembly 3900.
A method of detection, which is a method of non-disease diagnosis as a target, comprising the steps of:
s100, the diluent bin 4000 is placed in the second insertion space 2210 in advance, the puncture tube does not puncture the puncture membrane (in S100, the puncture tube can puncture the puncture membrane, and the kit forms a closed environment at the moment, namely, pollutants are prevented from entering the sample adding reaction bin from the puncture tube);
s200, moving the horizontal telescopic rod assembly, so that the jacking mechanism assembly drives the vibration motor and the heating block to descend, and the vibration motor and the heating block are lower than the horizontal separation plate 3703;
s300, pressing the vertical button assembly 3800 to push the reagent card assembly 2000 horizontally into the incubator assembly 3000, and after the reagent card assembly 2000 is in place, loosening the vertical button assembly 3800, the vertical button assembly 3800 fixing the reagent card assembly 2000;
s400, tearing off the reagent card cover 2300, aligning N adopting pipes with N liquid inlets of the liquid inlet assembly 2102, and enabling a sample to flow into the sample adding reaction bin 2202;
s500, the horizontal telescopic rod assembly moves, so that the jacking mechanism assembly drives the vibration motor and the heating block to ascend, and the heating block is attached to the vibration motor and the reagent card assembly;
s600, after the reaction is finished, the horizontal telescopic rod assembly reciprocates, and the diluent bin pushing component 3600 reciprocally presses the diluent bin 4000, so that diluent in the diluent bin 4000 enters the sample adding reaction bin 2202;
s700, the mixed liquid in the sample adding reaction bin contacts with a test strip along a reaction test channel 2201, and chromatographic reaction shows the result.
The above examples are provided for convenience of description of the present invention and are not to be construed as limiting the invention in any way, and any person skilled in the art will make partial changes or modifications to the invention by using the disclosed technical content without departing from the technical features of the invention.
Claims (10)
1. A chromatography kit, characterized in that the chromatography kit comprises: a reagent card assembly, a diluent bin;
the reagent card assembly comprises: a reagent card upper shell, a reagent card base and N reaction test strips; n is a natural number greater than or equal to 1;
a, the upper surface of reagent card epitheca is provided with: n observation windows and liquid inlet components; the liquid inlet assembly is provided with N liquid inlets;
b, the reagent card base includes: n reaction test channels; the N reaction test strips, the N reaction test channels and the N observation windows are arranged in one-to-one correspondence;
c, the lower surface of reagent card base still is provided with: a second insertion space; the second insertion space is used for inserting the diluent bin;
d, the reagent card base still includes: n diluent connecting channels and N puncture tubes; one end of the diluent connecting channel is communicated with the sample adding reaction bin, and the other end of the diluent connecting channel is connected with a puncture tube; the puncture tube is used for being connected with the diluent bin.
2. A chromatographic kit as claimed in claim 1, wherein: the diluent connecting channel adopts Z-shaped design.
3. An incubator assembly adapted to the chromatographic kit of claim 1, comprising: the incubator comprises an incubator base, a horizontal telescopic rod assembly, a jacking mechanism assembly, a vibration motor, a heating block and a diluent bin pushing member;
wherein, horizontal telescopic link subassembly includes: the horizontal telescopic rod is used for fixing the metal plate, the horizontal telescopic rod is used for moving the rod and the horizontal moving block; the horizontal telescopic rod fixing metal plate is fixedly arranged on the incubator base; the fixed end of the movable rod of the horizontal telescopic rod is fixedly connected with the fixed metal plate of the horizontal telescopic rod, and the movable end of the movable rod is connected with the horizontal moving block;
wherein, climbing mechanism subassembly includes: the device comprises a bottom support column, a first fixed platform, a second vertical support column, a second lifting platform, a vertical jacking component, a horizontal driving component and a vertical spring component; the first fixed platform is arranged above the incubator base, and a bottom support column is arranged between the first fixed platform and the incubator base; a second vertical supporting column is fixedly arranged on the upper surface of the first fixed platform, a vertical guide hole is formed in the second lifting platform, and the second vertical supporting column penetrates through the vertical guide hole; the upper part of the second lifting platform is fixed with a vertical spring assembly; the vertical jacking component is fixed with the second lifting platform;
wherein, the cooperation relation of horizontal telescopic link subassembly and climbing mechanism subassembly is: the horizontal driving component and the horizontal moving block are fixedly connected into a whole; the horizontal driving member is horizontally movable on the first fixed platform; the contact surfaces of the horizontal driving member and the vertical jacking member are inclined planes, and when the horizontal driving member moves horizontally, the vertical jacking member can move vertically;
the vibration motor is arranged on the upper surface of the second lifting platform;
wherein the top of the vertical spring assembly is fixedly provided with the heating block;
wherein the diluent cartridge pushing member is fixedly provided at an upper portion of the horizontal moving block for reciprocally pressing the diluent cartridge of the chromatography kit of claim 1.
4. An incubator assembly according to claim 3, wherein the horizontal telescopic rod assembly further comprises: the device comprises a first guide column fixing seat, a horizontal guide rod and a second guide column fixing seat; the first guide post fixing seat and the second guide post fixing seat are fixedly arranged on the incubator base; the horizontal guide rod is fixedly arranged between the first guide column fixing seat and the second guide column fixing seat; the horizontal moving block is provided with a guide hole, and the horizontal guide rod passes through the guide hole; the direction of the horizontal guide rod is parallel to the direction of the movable rod of the horizontal telescopic rod.
5. An incubator assembly according to claim 3, wherein the incubator assembly further comprises: an incubator assembly housing; the incubator assembly housing comprises: a first space, a second space, and a horizontal partition plate; the incubator base is adapted to the bottom of the incubator assembly enclosure; the horizontal separation plate is also provided with a strip-shaped hole, and the diluent bin pushing member passes through the strip-shaped hole;
in the initial state, the horizontal telescopic rod assembly, the jacking mechanism assembly, the vibration motor and the heating block are all arranged in the second space;
the bottom of the incubator assembly housing is adapted to the incubator base.
6. The incubator assembly of claim 5, wherein the incubator assembly further comprises: a vertical button assembly and a horizontal ejection assembly;
wherein, vertical button subassembly includes: the first guide post is connected with the first pressing component; the pressing buckle plate is horizontally arranged, and the first spring, the second guide column and the second pressing member are vertically arranged;
the upper surface of the fixer is fixed with the lower end of the second guide post; the lower end of the second pressing component is provided with a groove extending into the second pressing component; the lower end of the first spring is fixed with the pressing buckle plate, and the upper end of the first spring is fixed with the lower surface of the fixer; the upper ends of the second springs and the second guide posts are inserted into grooves formed in the lower ends of the second pressing members;
wherein, the horizontal ejection assembly includes: a top cap pressing plate, an ejection spring and a top cap; the top cap pressing plate is a vertical plate, and the top cap and the ejection spring are horizontally arranged; one end of the ejection spring is fixed with the top cap pressing plate, and the other end of the ejection spring is fixed with the top cap; the top cap is provided with a concave space, and the ejection spring extends into the concave space;
the top cap pressing plate and the pressing buckle plate are fixedly connected with the incubator assembly shell.
7. A chromatographic kit as claimed in claim 1 wherein the diluent compartment comprises: the device comprises a diluent bin upper seat, a diluent bin base, N piston silica gel valves, N springs and N puncture membranes;
the upper seat of the diluent bin comprises N storage bins and N insertion bins;
the lower end part of the insertion bin adopts a step part design;
the diluent storehouse base includes: an outer shell, wherein N diluent inserting pipes are arranged in the outer shell;
the upper base of the diluent bin is inserted into the base of the diluent bin: the insertion bin is inserted into a gap between the outer shell and the insertion tube, and the insertion tube is inserted into the insertion bin;
a piston silica gel valve is arranged at the end part of the insertion tube inserted into the insertion bin, and the puncture membrane is arranged at the outer side of the other end part; one end of the spring is fixed with the bottom of the outer shell, and the other end of the spring is fixed with the step part of the insertion bin;
wherein the diluent bin is horizontally inserted into a second insertion space of the reagent card assembly; the puncture tube can be inserted into the insertion tube.
8. The chromatography kit of claim 7, further comprising: an incubator assembly as recited in claim 6; the reagent card assembly is placed on the first space of the incubator assembly;
the reagent card assembly further comprises: a reagent card cover; the reagent card cover is used for sealing the liquid inlet assembly;
the reagent card base further comprises: the first sliding groove, the second sliding groove, the first concave space and the third concave space; the first concave space is used for placing a motor and a heating block; the third concave space is used for the movement of the liquid releasing bin pushing component; the first sliding groove is arranged on the outer side of the reagent card base; the inner side walls of the first concave space and the second insertion space are provided with second sliding grooves; the reagent card base is also provided with a reagent card component fixing groove; the holder is insertable into the reagent card assembly holding slot;
the diluent bin base further comprises: an outer housing boss;
the incubator assembly housing further comprises: a gear plate and a protruding block; a protruding block is arranged on the side wall of the first space and is matched with the first sliding groove; the upper surface at horizontal division board still is provided with two vertical fender boards, the fender board with outer housing boss cooperation is used for spacing to the diluent storehouse.
9. A method for detecting a non-disease diagnosis, comprising the steps of:
s100, a diluent bin is placed in a second insertion space in advance;
s200, aligning N adopting pipes to N liquid inlets of a liquid inlet assembly, and enabling samples to flow into a sample adding reaction bin for isothermal amplification reaction;
s300, after the reaction is finished, pressing the diluent bin reciprocally to enable diluent in the diluent bin to enter the sample adding reaction bin;
s400, the mixed liquid in the sample adding reaction bin contacts with a test strip along a reaction test channel, and chromatographic reaction shows a result.
10. A method for detecting a non-disease diagnosis, comprising the steps of:
s100, a diluent bin is placed in a second insertion space in advance;
s200, the horizontal telescopic rod assembly moves, so that the jacking mechanism assembly drives the vibration motor and the heating block to descend, and the vibration motor and the heating block are lower than the horizontal separation plate;
s300, pressing the vertical button assembly, horizontally pushing the reagent card assembly into the incubator assembly, loosening the vertical button assembly after the reagent card assembly is in place, and fixing the reagent card assembly by the vertical button assembly;
s400, tearing off the reagent card cover, aligning N adopting pipes with N liquid inlets of the liquid inlet assembly, and allowing samples to flow into the sample adding reaction bin;
s500, the horizontal telescopic rod assembly moves, so that the jacking mechanism assembly drives the vibration motor and the heating block to ascend, and the heating block is attached to the vibration motor and the reagent card assembly;
s600, after the reaction is finished, the horizontal telescopic rod assembly reciprocates, and the diluent bin pushing component reciprocally presses the diluent bin, so that diluent in the diluent bin enters the sample adding reaction bin;
s700, the mixed liquid in the sample adding reaction bin contacts with a test strip along a reaction test channel, and chromatographic reaction shows a result.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311555253.0A CN117264748B (en) | 2023-11-21 | 2023-11-21 | Reagent card assembly, incubator assembly, chromatographic kit and detection method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311555253.0A CN117264748B (en) | 2023-11-21 | 2023-11-21 | Reagent card assembly, incubator assembly, chromatographic kit and detection method |
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| CN117264748B true CN117264748B (en) | 2024-01-30 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520536A (en) * | 2016-10-17 | 2017-03-22 | 西安交通大学 | Nucleic-acid extraction, amplification and detection integrated device and manufacturing method and detection method thereof |
| CN115141745A (en) * | 2022-09-01 | 2022-10-04 | 苏州雅睿生物技术股份有限公司 | Multi-channel split type kit, nucleic acid detection device and detection method |
| CN115892715A (en) * | 2022-12-12 | 2023-04-04 | 青岛瑞斯凯尔生物科技有限公司 | Detection kit |
| CN219348878U (en) * | 2023-03-13 | 2023-07-14 | 天津柏稷生物科技有限公司 | Single-person immunoassay reagent card |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520536A (en) * | 2016-10-17 | 2017-03-22 | 西安交通大学 | Nucleic-acid extraction, amplification and detection integrated device and manufacturing method and detection method thereof |
| CN115141745A (en) * | 2022-09-01 | 2022-10-04 | 苏州雅睿生物技术股份有限公司 | Multi-channel split type kit, nucleic acid detection device and detection method |
| CN115892715A (en) * | 2022-12-12 | 2023-04-04 | 青岛瑞斯凯尔生物科技有限公司 | Detection kit |
| CN219348878U (en) * | 2023-03-13 | 2023-07-14 | 天津柏稷生物科技有限公司 | Single-person immunoassay reagent card |
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