CN219326774U

CN219326774U - Microfluidic detection device and nucleic acid detection equipment

Info

Publication number: CN219326774U
Application number: CN202320147893.7U
Authority: CN
Inventors: 刘林波; 蒋析文; 喻泽雄; 舒海涛; 黎俊杰
Original assignee: Guangzhou Da'an Gene Co ltd
Current assignee: Guangzhou Da'an Gene Co ltd
Priority date: 2023-02-02
Filing date: 2023-02-02
Publication date: 2023-07-11
Anticipated expiration: 2033-02-02

Abstract

The embodiment of the application belongs to the technical field of medical detection, and relates to a microfluidic detection device and nucleic acid detection equipment. The microfluidic detection device comprises: the device comprises a shell, wherein a functional cavity, a first sample injection assembly, a second sample injection assembly, a fluid quantifying tank, a capillary absorption part and a result display assembly are arranged in the shell; the first sample injection assembly and the second sample injection assembly are both communicated with the functional cavity, the functional cavity is communicated with the fluid quantifying tank, the fluid quantifying tank is communicated with the result display assembly through the capillary absorption part, and the result display assembly is used for displaying a detection result. According to the liquid quantitative tank control device, the water absorption capacity of the result display assembly is controlled through the liquid quantitative tank, and the liquid in the liquid quantitative tank is slowly chromatographed to the result display assembly through the capillary absorption part, so that the effect of accurately controlling the water absorption capacity on the test paper strip is achieved.

Description

Microfluidic detection device and nucleic acid detection equipment

Technical Field

The present application relates to the field of medical detection technology, and more particularly, to a microfluidic detection device and a nucleic acid detection apparatus.

Background

Along with the development of scientific technology, the molecular diagnosis technology has been widely applied to aspects of epidemic investigation, infectious disease diagnosis, food hygiene inspection and the like, and a series of detection instruments, such as a constant temperature amplification instrument, are correspondingly presented, and the PCR instrument has gradually replaced the traditional smear or microscopic examination mode.

The steps of extracting and purifying nucleic acid, amplifying nucleic acid, hybridizing nucleic acid molecule, etc. are all carried out separately, and for example, the sample preparation instrument for extracting nucleic acid, the nucleic acid extraction instrument, the PCR instrument for amplifying, etc. need to be carried out separately. The whole nucleic acid detection mainly comprises the following steps: manually extracting or manually adding the sample into a full-automatic nucleic acid extractor to extract nucleic acid and purify, manually transferring the purified nucleic acid solution to a nucleic acid amplification instrument, amplifying the nucleic acid by using the nucleic acid amplification instrument, and finally transferring the amplified product to full-automatic analysis. The whole detection process has the advantages of complex equipment, huge volume, low detection efficiency, poor flexibility and expensive instrument and equipment, so that the whole operation process is complicated, high-professional-level personnel are required for operation, and the detection is required to be operated by skilled technicians, so that the portable detection of families or other places cannot be realized.

Disclosure of Invention

The embodiment of the application provides a microfluidic detection device and nucleic acid detection equipment, which are used for solving the technical problems that in the prior art, the nucleic acid detection process is complex, the operation is complex, and the detection is finished by specialized staff.

In order to solve the above technical problems, the embodiments of the present application provide a microfluidic detection device, which adopts the following technical schemes:

a microfluidic detection device comprising: the device comprises a shell, wherein a functional cavity, a first sample injection assembly, a second sample injection assembly, a fluid quantifying tank, a capillary absorption part and a result display assembly are arranged in the shell; the first sample injection assembly and the second sample injection assembly are both communicated with the functional cavity, the functional cavity is communicated with the fluid quantifying tank, the fluid quantifying tank is communicated with the result display assembly through the capillary absorption part, and the result display assembly is used for displaying a detection result.

Further, the shell comprises a containing shell, a clamping plate and a top plate, wherein the clamping plate is arranged on the containing shell, and the top plate is arranged on the clamping plate;

the functional chamber is arranged on the containing shell and the clamping plate;

the first sample injection assembly is arranged on the containing shell and the clamping plate, or the first sample injection assembly is arranged on the clamping plate, and the second sample injection assembly is arranged on the top plate;

The fluid quantifying tank is arranged on one surface of the clamping plate, which is far away from the accommodating shell;

the capillary absorption part is arranged on one surface of the top plate facing the clamping plate, and is communicated with the fluid quantifying tank;

the result display assembly is arranged on the top plate and the clamping plate.

Further, the capillary suction member includes a first suction plate and a second suction plate; the first adsorption plate and the second adsorption plate are arranged on one surface of the top plate, which faces the clamping plate, at intervals, and a gap between the first adsorption plate and the second adsorption plate forms an adsorption groove; one ends of the first adsorption plate and the second adsorption plate extend downwards to the fluid quantitative tank, and the other ends of the first adsorption plate and the second adsorption plate are abutted to the result display assembly.

Further, the surface of the top plate is a hydrophilic layer; alternatively, the surface of the capillary adsorbing piece is hydrophilic.

Further, the functional chamber comprises a first chamber and a second chamber, wherein the first chamber is arranged on the accommodating shell, and the second chamber is arranged on the clamping plate; the first sample injection assembly is arranged in the first cavity and communicated with the second cavity, and the second cavity is communicated with the first cavity and the fluid quantitative tank.

Further, the second chamber comprises a first communication cavity, a second communication cavity and a third communication cavity; the second sample injection assembly is provided with a second tributary close to one end of the first communication cavity, the second communication cavity and the third communication cavity, and the second tributary is corresponding to the first communication cavity, the second communication cavity and the third communication cavity in number and is respectively communicated with the first communication cavity, the second communication cavity and the third communication cavity;

the first chamber comprises a first reaction cavity, a second reaction cavity and a waste liquid pool; the first sample injection assembly is provided with a first branch flow at one end close to the first reaction cavity, the second reaction cavity and the waste liquid pool, and the first branch flow is corresponding to the first reaction cavity, the second reaction cavity and the waste liquid pool in number and is respectively communicated with the first branch flow;

the first communication cavity is communicated with the first reaction cavity, the second communication cavity is communicated with the second reaction cavity, the third communication cavity is communicated with the waste liquid pool, and the first communication cavity and the second communication cavity are communicated with the fluid quantitative pool.

Further, the number of the fluid quantifying tanks is two, and the two fluid quantifying tanks are respectively communicated with the first communicating cavity and the second communicating cavity; and/or the number of the groups of groups,

the total number of the first reaction cavity, the second reaction cavity and the waste liquid pool is even; and/or the number of the groups of groups,

The volume of the first reaction cavity is consistent with the volume of the second reaction cavity.

Further, a baffle is arranged in the second chamber, extends into the first chamber and is arranged at intervals with the bottom of the first chamber; and/or the number of the groups of groups,

the clamping plate is provided with a mixing channel which is communicated with the fluid quantitative tank and the second chamber.

Further, the second sample injection assembly comprises a second puncture structure, a second sample injection hole, a second micro-channel and a liquid sac; the second puncture structure is arranged on one surface of the top plate away from the clamping plate, the second sample injection hole penetrates through the top plate and is positioned in the second puncture structure, the second micro-flow channel is arranged on one surface of the top plate towards the clamping plate, the second micro-flow channel is communicated with the second sample injection hole and the functional cavity, and the liquid sac is arranged on the top plate and is positioned above the second puncture structure.

Further, the first sample injection assembly comprises a first puncture structure, an alignment structure, a first sample injection hole and a first micro-channel; the first puncture structure and the alignment structure are arranged on one surface of the clamping plate, which is far away from the accommodating shell, the alignment structure is provided with a positioning groove, the first puncture structure is positioned in the alignment structure, and the first sample injection hole penetrates through the clamping plate and is positioned in the first puncture structure;

The first micro-flow channel is arranged on one surface of the accommodating shell facing the clamping plate, or is arranged on one surface of the clamping plate facing the accommodating shell, and is communicated with the first sample injection hole and the functional cavity; the top plate is provided with a mounting hole, and the first puncture structure and the alignment structure are positioned in the mounting hole.

Further, the result display assembly comprises a containing groove, an observation window and a detection piece; the holding groove is located splint keep away from the one side of holding shell, the detection spare is located in the holding groove, the observation window is located on the roof and with the holding groove corresponds, the one end of capillary absorption spare with the detection spare butt.

Further, the accommodating shell comprises a first shell and a second shell, and the first shell is arranged on the second shell; the functional chamber and the first sample injection assembly are arranged on the clamping plate and the first shell.

Further, the microfluidic detection device further comprises a heating component, a temperature sensor and a PCB board; the heating assembly comprises a heating module and a temperature control module, the heating module is arranged on one face of the first shell, which faces the second shell, the heating module corresponds to the position of the functional cavity, the temperature control module and the PCB are arranged on the first shell, the temperature sensor is arranged in the functional cavity, and the heating assembly is electrically connected with the PCB.

In order to solve the above technical problems, embodiments of the present application provide a nucleic acid detecting apparatus, which adopts the following technical solutions:

a nucleic acid detection apparatus comprising a quantitative sample-adding device and a microfluidic detection device as described above;

the quantitative sample adding device comprises a cylinder body, a liquid storage pipe, a pipe cover and a piston rod; the cylinder body comprises a storage cavity and a movable cavity which are mutually communicated, the cylinder wall of the cylinder body is provided with a liquid inlet which is communicated with the movable cavity, and the liquid storage pipe is arranged at the liquid inlet; one end of the piston rod penetrates out of the movable cavity, and the other end of the piston rod is used for sealing the liquid inlet and is arranged in the movable cavity in a sliding manner; the tube cover is movably connected with one end of the cylinder body, which is far away from the piston rod, and is used for sealing the storage cavity; the tube cover is connected with the first sample injection assembly.

Compared with the prior art, the embodiment of the application has the following main beneficial effects:

the first sample injection assembly and the second sample injection assembly are used for carrying out twice sample injection, so that accurate control is realized, and the method has the advantages of simplicity in operation, safety, reliability, high detection efficiency, high sensitivity, less sample consumption and the like; can detect a plurality of different biological samples and has a very wide application range.

In this embodiment, the water absorption capacity of the result display assembly is controlled by the fluid quantifying tank, and the liquid in the fluid quantifying tank is slowly chromatographed to the result display assembly by the capillary absorption member, so that the effect of precisely controlling the water absorption capacity on the test strip is achieved.

Drawings

For a clearer description of the solution of the present application, a brief introduction will be given to the drawings needed in the description of the embodiments, which are some embodiments of the present application, and from which other drawings can be obtained for a person skilled in the art without the inventive effort.

FIG. 1 is a schematic diagram of a nucleic acid detecting apparatus according to an embodiment of the present application;

FIG. 2 is a schematic view of the structure of the top plate of FIG. 1;

FIG. 3 is a schematic view of the structure of the splint of FIG. 1;

FIG. 4 is a schematic view of the structure of FIG. 3 from another perspective;

FIG. 5 is a schematic view of the first housing of FIG. 1;

FIG. 6 is a schematic view of the structure of FIG. 5 from another perspective;

fig. 7 is a schematic structural view of the second housing of fig. 1.

Reference numerals:

100. a microfluidic detection device;

1. a housing; 11. a housing case; 111. a first housing; 1111. a first buckle; 1112. a support column; 1113. a first mounting groove; 1114. a usb interface; 1115. a second buckle; 1116. a positioning structure; 1117. a second mounting groove; 112. a second housing; 1121. a snap groove; 1122. a fixing groove; 12. a clamping plate; 121. a mixing channel; 13. a top plate; 131. a mounting hole;

2. A functional chamber; 21. a first chamber; 211. a first reaction chamber; 212. a second reaction chamber; 213. a waste liquid pool; 22. a second chamber; 221. a first communication chamber; 222. a second communication chamber; 223. a third communication chamber; 23. a baffle;

3. a first sample injection assembly; 31. a first puncturing structure; 32. an alignment structure; 321. a positioning groove; 33. a first sample injection hole; 34. a first microchannel; 35. a first substream;

4. a second sample injection assembly; 41. a second piercing structure; 42. a second sample injection hole; 43. a second microchannel; 44. a fluid sac; 45. a second substream;

5. a fluid dosing tank; 6. a capillary suction member; 61. a first adsorption plate; 62. a second adsorption plate; 63. an adsorption tank; 7. a result display component; 71. a receiving groove; 72. an observation window; 8. a heating module; 9. a temperature control module;

200. a quantitative sample adding device;

201. a cylinder; 202. a storage chamber; 203. a movable cavity; 204. a liquid inlet; 205. a liquid storage tube; 206. a tube cover; 207. a piston rod.

Detailed Description

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the description of the applications herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having" and any variations thereof in the description and claims of the present application and in the description of the figures above are intended to cover non-exclusive inclusions. The terms first, second and the like in the description and in the claims or in the above-described figures, are used for distinguishing between different objects and not necessarily for describing a sequential or chronological order.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.

In order to better understand the technical solutions of the present application, the following description will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the accompanying drawings.

An embodiment of the present application provides a microfluidic detection device 100, as shown in fig. 1 to 3, where the microfluidic detection device 100 includes: the device comprises a shell 1, wherein a functional chamber 2, a first sample injection assembly 3, a second sample injection assembly 4, a fluid quantitative tank 5, a capillary absorption member 6 and a result display assembly 7 are arranged in the shell 1; the first sample injection assembly 3 and the second sample injection assembly 4 are both communicated with the functional cavity 2, the functional cavity 2 is communicated with the fluid quantifying tank 5, the fluid quantifying tank 5 is communicated with the result display assembly 7 through the capillary absorption member 6, and the result display assembly 7 is used for displaying detection results.

In the microfluidic detection device 100 provided in this embodiment, the first sample injection component 3 and the second sample injection component 4 can respectively perform nucleic acid amplification sample addition and diluent sample addition, the nucleic acid amplification reaction solution flows into the functional chamber 2 through the first sample injection component 3, and the nucleic acid amplification reaction solution and the pre-installed reaction reagent in the functional chamber 2 perform biochemical reaction; the diluent flows into the functional chamber 2 through the second sample injection component 4, the liquid flows into the fluid quantifying tank 5 after filling the functional chamber 2, the liquid is slowly chromatographed to the result display component 7 through the capillary absorption component 6, and finally the biochemical reaction result is checked through the result display component 7.

The microfluidic detection device 100 provided by the embodiment separately performs the nucleic acid amplification sample adding and the dilution of the reaction liquid through the first sample injection assembly 3 and the second sample injection assembly 4, realizes the accurate control of sample adding and detection, and has the advantages of simple operation, safety, reliability, high detection efficiency, high sensitivity, less sample consumption and the like; the nucleic acid amplification sample can detect a plurality of different biological samples, and has a very wide application range.

In this embodiment, the water absorption capacity of the result display assembly 7 is controlled by the fluid quantifying tank 5, and the liquid in the fluid quantifying tank 5 is slowly chromatographed to the result display assembly 7 by the capillary suction device 6, so that the effect of precisely controlling the water absorption capacity on the test strip is achieved.

In the embodiment, the whole reaction process is sealed without uncovering and sample adding, so that aerosol pollution in the nucleic acid amplification process is avoided.

The functional chamber 2 is a chamber in which the detection reagent undergoes biochemical reaction, and is not limited by the type of detection and the detection object; the pre-filled reaction reagent in the functional chamber 2 refers to reagent materials used in biochemical detection, and is usually preset in the functional chamber 2, and according to different detection targets, the components contained in the materials and the pre-filling method can be determined in various ways according to the required environment of the materials, such as freeze-dried balls, cotton sheets and the like.

As shown in fig. 1 to 7, further, the housing 1 includes a housing case 11, a clamping plate 12, and a top plate 13, the clamping plate 12 is provided on the housing case 11, and the top plate 13 is provided on the clamping plate 12;

the functional chamber 2 is arranged on the containing shell 11 and the clamping plate 12;

the first sample injection assembly 3 is arranged on the containing shell 11 and the clamping plate 12, or the first sample injection assembly 3 is arranged on the clamping plate 12, and the second sample injection assembly 4 is arranged on the top plate 13;

the fluid quantifying tank 5 is arranged on one surface of the clamping plate 12 away from the accommodating shell 11;

The capillary absorption part 6 is arranged on one surface of the top plate 13 facing the clamping plate 12, and the capillary absorption part 6 is communicated with the fluid quantifying tank 5;

the result display assembly 7 is arranged on the top plate 13 and the clamping plate 12.

In this embodiment, divide into holding shell 11, splint 12 and roof 13 with shell 1, make first advance appearance subassembly 3 and second advance appearance subassembly 4 separate the setting, make the dilution of nucleic acid amplification increase appearance and reaction solution go on separately, realized the accurate of application of sample and detection and controlled.

In this embodiment, the fluid quantifying tank 5 is disposed on a surface of the clamping plate 12 away from the accommodating shell 11, the capillary absorption member 6 is disposed on a surface of the top plate 13 facing the clamping plate 12 and is communicated with the fluid quantifying tank 5, and the position setting is reasonable, so that the volume of the microfluidic detection device 100 is reduced, and the liquid in the fluid quantifying tank 5 is guaranteed to be slowly chromatographed on the result display assembly 7, so that the effect of accurately controlling the water absorption amount on the test strip is achieved.

Further, a sealing structure is formed by bonding the top plate 13 and the clamping plate 12, and the top plate 13 and the clamping plate 12 are connected by laser welding, ultrasonic welding, thermal compression bonding, plasma bonding, solvent bonding or adhesive bonding.

Further, a sealing structure is formed between the accommodating shell 11 and the clamping plate 12 in a fitting manner, and the accommodating shell 11 and the clamping plate 12 are connected in an interference buckling or adhesive bonding manner.

As shown in fig. 2, further, the capillary suction device 6 includes a first suction plate 61 and a second suction plate 62; the first adsorption plate 61 and the second adsorption plate 62 are arranged on one surface of the top plate 13 facing the clamping plate 12 at intervals, and an adsorption groove 63 is formed in a gap between the first adsorption plate 61 and the second adsorption plate 62; one ends of the first adsorption plate 61 and the second adsorption plate 62 extend downward to the fluid quantification basin 5, and the other ends of the first adsorption plate 61 and the second adsorption plate 62 are abutted against the result display assembly 7.

In the present embodiment, the adsorption grooves 63 are formed by the first adsorption plates 61 and the second adsorption plates 62 that are arranged at intervals, so as to avoid grooving on the top plate 13 and reduce the processing difficulty; by providing the first adsorption plate 61 and the second adsorption plate 62 with one end extending downward to the fluid quantification tank 5, it is ensured that the adsorption groove 63 can adsorb the liquid in the fluid quantification tank 5, and the other ends of the first adsorption plate 61 and the second adsorption plate 62 are abutted against the result display module 7, so that when the liquid flows into the fluid quantification tank 5 after filling the functional chamber 2, the liquid can be slowly chromatographed to the result display module 7 through the adsorption groove 63.

Specifically, one ends of the first and

second adsorption plates

61 and 62 extend downward to the bottom of the fluid-quantifying tank 5. To ensure that as the liquid in the fluid-quantifying tank 5 decreases, the adsorption tank 63 can still adsorb the liquid in the fluid-quantifying tank 5; since the adsorption groove 63 is formed of the first adsorption plate 61 and the second adsorption plate 62 which are disposed at intervals, one ends of the first adsorption plate 61 and the second adsorption plate 62 extend downward to the bottom of the fluid-quantifying tank 5, and adsorption can be performed.

In this embodiment, the width of the adsorption tank 63 is 10 μm to 1.5mm. Namely, the gap between the first suction plate 61 and the second suction plate 62 is 10 μm to 1.5mm; the width of the adsorption groove 63 may be any one or any two of 10 μm, 20 μm, 30 μm, 40 μm, 50 μm, 60 μm, 70 μm, 80 μm, 90 μm, 1mm, 1.1mm, 1.2mm, 1.3mm, 1.4mm, 1.5mm, etc., and the present application is not limited thereto.

Preferably, the width of the adsorption groove 63 is 10 μm; the smaller the width of the adsorption groove 63 is, the better the capillary action thereof is.

Further, the surface of the top plate 13 is hydrophilic; alternatively, the surface of the capillary absorption member 6 is hydrophilic. In this embodiment, the surface of the top plate 13 or the capillary suction device 6 is hydrophilic to ensure that the liquid in the fluid dosing tank 5 can be slowly chromatographed through the capillary suction device 6 into the result display assembly 7.

Specifically, the top plate 13 or the capillary suction device 6 is made of a hydrophilic material; so that the surface of the top plate 13 or the capillary suction device 6 is hydrophilic.

Specifically, the hydrophilic material is polypropylene (PP), polystyrene (PS), or the like, and the present application is not limited herein.

Specifically, the contact angle of the hydrophilic material is between 0 and 60 degrees.

As shown in fig. 3 to 6, further, the functional chamber 2 includes a first chamber 21 and a second chamber 22, the first chamber 21 is provided on the housing shell 11, and the second chamber 22 is provided on the clamping plate 12; the first sample injection assembly 3 is arranged in the first chamber 21 to be communicated, the second sample injection assembly 4 is communicated with the second chamber 22, and the second chamber 22 is communicated with the first chamber 21 and the fluid quantifying tank 5.

In this embodiment, the nucleic acid amplification reaction solution flows into the first chamber 21 through the first sample injection assembly 3, and the nucleic acid amplification reaction solution performs a biochemical reaction with the pre-loaded reaction reagent in the first chamber 21; the diluent flows into the second chamber 22 through the second sample injection assembly 4, the diluent is mixed with the nucleic acid amplification reaction liquid due to the communication between the first chamber 21 and the second chamber 22, after the liquid fills the first chamber 21 and the second chamber 22, the diluent flows into the fluid quantifying tank 5 from the second chamber 22, the liquid is slowly chromatographed to the result display assembly 7 through the capillary absorption assembly 6, and finally the biochemical reaction result is checked through the result display assembly 7.

In this embodiment, the dilution of the nucleic acid amplification reaction solution and the sample addition are separately performed by the first sample injection assembly 3 and the second sample injection assembly 4, so that accurate control of sample addition and detection is realized, and the mixing of the nucleic acid amplification reaction solution and the diluent is realized by the first chamber 21 and the second chamber 22.

Further, the first chamber 21 and the second chamber 22 are connected by means of laser welding, ultrasonic welding, thermocompression bonding, plasma bonding, solvent bonding, or adhesive bonding.

As shown in fig. 2 to 6, further, the second chamber 22 includes a first communication chamber 221, a second communication chamber 222, and a third communication chamber 223; the second sample injection assembly 4 is provided with a second tributary 45 near one end of the first communication cavity 221, the second communication cavity 222 and the third communication cavity 223, and the second tributary 45 corresponds to the number of the first communication cavity 221, the second communication cavity 222 and the third communication cavity 223 and is respectively communicated with the second tributary 45;

the first chamber 21 includes a first reaction chamber 211, a second reaction chamber 212, and a waste liquid tank 213; a first tributary 35 is arranged at one end of the first sample injection assembly 3 near the first reaction cavity 211, the second reaction cavity 212 and the waste liquid tank 213, and the first tributary 35 is corresponding to and respectively communicated with the first reaction cavity 211, the second reaction cavity 212 and the waste liquid tank 213;

The first communicating chamber 221 communicates with the first reaction chamber 211, the second communicating chamber 222 communicates with the second reaction chamber 212, the third communicating chamber 223 communicates with the waste liquid tank 213, and the first communicating chamber 221 and the second communicating chamber 222 communicate with the fluid dosing tank 5.

In this embodiment, the nucleic acid amplification reaction solution is split into the first reaction chamber 211, the second reaction chamber 212 and the waste liquid tank 213 through the first branch flow 35 of the first sample injection assembly 3, the nucleic acid amplification reaction solution performs biochemical reaction with the pre-installed reaction reagents in the first reaction chamber 211 and the second reaction chamber 212, and the nucleic acid amplification reaction solution flowing into the waste liquid tank 213 does not participate in the reaction; the diluent is split into the first communication cavity 221, the second communication cavity 222 and the third communication cavity 223 through the second branch flow 45 of the second sample injection assembly 4, and the diluent is mixed with the nucleic acid amplification reaction liquid and flows into the third communication cavity 223 into the waste liquid tank 213 because the first communication cavity 221 is communicated with the first reaction cavity 211, the second communication cavity 222 is communicated with the second reaction cavity 212, and the third communication cavity 223 is communicated with the waste liquid tank 213; after the liquid fills the first chamber 21 and the second chamber 22, the liquid flows from the first communication cavity 221 and the second communication cavity 222 to the fluid quantitative tank 5, slowly chromatographs to the result display component 7 through the capillary absorption component 6, and finally checks the biochemical reaction result through the result display component 7.

In this embodiment, the waste liquid pool 213 is used to make the diversion more stable and uniform, and can store redundant nucleic acid amplification reaction liquid and diluent, and a large amount of nucleic acid amplification reaction liquid and diluent gushes into the fluid quantifying pool 5, so as to achieve the effect of accurately controlling the water absorption on the test strip.

As shown in fig. 3 and 4, further, the number of the fluid-quantifying tanks 5 is two, and the two fluid-quantifying tanks 5 are respectively communicated with the first communicating chamber 221 and the second communicating chamber 222; to achieve splitting.

Further, the total number of the first reaction chamber 211, the second reaction chamber 212 and the waste liquid tank 213 is an even number, such as 2, 4, 8, 16 … 2 ⁿ The method comprises the steps of carrying out a first treatment on the surface of the So that the splitting of the first substream 35 of the first injection assembly 3 is more stable and uniform.

Further, the volume of the first reaction chamber 211 corresponds to the volume of the second reaction chamber 212; to ensure that the amount of liquid in the first reaction chamber 211 is consistent with the amount of liquid in the second reaction chamber 212, thereby making the split flow more stable and uniform.

Illustratively, the number of first reaction chambers 211 is one, the number of second reaction chambers 212 is two, the number of waste liquid pools 213 is one, the sum of the volumes of the two second reaction chambers 212 corresponds to the sum of the volumes of one first reaction chamber 211, the two second reaction chambers 212 are communicated with the second communication chamber 222, and one first reaction chamber 211 is communicated with the first communication chamber 221.

Illustratively, the first substream 35 and the second substream 45 are four; the four first branch flows 35 are respectively communicated with a first reaction cavity 211, two second reaction cavities 212 and a waste liquid pool 213; two of the four second subsidiary streams 45 communicate with the second communication chamber 222 in which the two second reaction chambers 212 communicate, and the remaining two second subsidiary streams 45 communicate with the first communication chamber 221 and the third communication chamber 223, respectively.

By adopting a one-to-four three-channel design, the nucleic acid amplification reaction liquid flows into a first reaction cavity 211, two second reaction cavities 212 and a waste liquid pool 213 through a first branch flow 35, so that the liquid can uniformly flow into the functional cavity 2 in one-to-one way, and the flow distribution is more stable and uniform; the diluent flows into the first communicating cavity 221, the third communicating cavity 223 and the two second communicating cavities 222 communicated with the two second reaction cavities 212 through the second branch flow 45, so that the liquid can uniformly flow into the functional chamber 2 in a one-to-one way, and the flow distribution is more stable and uniform.

As shown in fig. 3 and 4, further, a baffle 23 is disposed in the second chamber 22, and the baffle 23 extends into the first chamber 21 and is spaced from the bottom of the first chamber 21.

Further, a mixing channel 121 is provided on the clamping plate 12, and the mixing channel 121 is in communication with the fluid dosing tank 5 and the second chamber 22.

In this embodiment, the nucleic acid amplification reaction solution flows into the side of the first chamber 21 far from the fluid quantification pool 5 through the first sample injection assembly 3, the nucleic acid amplification reaction solution and the pre-loaded reaction reagent in the first chamber 21 perform biochemical reaction, and along with the increase of the nucleic acid amplification reaction solution, the nucleic acid amplification reaction solution flows into the side of the first chamber 21 near the fluid quantification pool 5 from the bottom gap between the baffle plate 23 and the first chamber 21; the diluent flows into the side of the second chamber 22 away from the fluid quantifying tank 5 through the second sample injection assembly 4, the diluent is mixed with the nucleic acid amplification reaction liquid due to the communication between the first chamber 21 and the second chamber 22, and the mixed liquid flows into the side of the first chamber 21 adjacent to the fluid quantifying tank 5 from the bottom gap between the baffle plate 23 and the first chamber 21 and fills the side of the second chamber 22 adjacent to the fluid quantifying tank 5 along with the increase of the diluent; subsequently, the mixed liquid flows from the mixing channel 121 into the fluid metering tank 5, then flows through the capillary suction device 6 to slowly chromatograph the liquid to the result display assembly 7, and finally, the biochemical reaction result is checked through the result display assembly 7.

In this embodiment, the baffle 23 prevents the diluent from entering and filling the second chamber 22, and flows into the mixing channel 121 without being fully mixed with the nucleic acid amplification reaction liquid, the mixed liquid in the first chamber 21 and the second chamber 22 enters the mixing channel 121 for further mixing, the reaction liquid after two steps of mixing enters the fluid quantifying tank 5, if the reaction liquid is not fully mixed, the reaction liquid enters the fluid quantifying tank 5 and is chromatographed to the result display component 7 by the capillary absorption member 6, and the detection precision is poor; the baffle plate 23 and the mixing channel 121 are designed to improve the mixing effect of the nucleic acid amplification reaction solution and the diluent to improve the detection accuracy.

In this embodiment, the mixing channel 121 prevents the flow rate of the liquid from being too high, so that the nucleic acid amplification reaction solution flows into the fluid quantification reservoir 5 without being sufficiently mixed with the diluent, and the insufficiently mixed liquid is chromatographed to the result display module 7 by the capillary suction device 6 after entering the fluid quantification reservoir 5 through the mixing channel 121, which may result in poor detection accuracy; the mixing channel 121 further provides mixing time for the nucleic acid amplification reaction solution and the diluent and can enhance the mixing effect to enhance the mixing effect of the nucleic acid amplification reaction solution and the diluent, thereby enhancing the detection accuracy.

As shown in fig. 1 and 2, the second sample injection assembly 4 further includes a second puncture structure 41, a second sample injection hole 42, a second micro flow channel 43, and a liquid sac 44; the second puncture structure 41 is disposed on a surface of the top plate 13 away from the clamping plate 12, the second sample injection hole 42 penetrates through the top plate 13 and is located in the second puncture structure 41, the second micro flow channel 43 is disposed on a surface of the top plate 13 facing the clamping plate 12, the second micro flow channel 43 is communicated with the second sample injection hole 42 and the functional chamber 2, and the liquid sac 44 is disposed on the top plate 13 and is located above the second puncture structure 41.

In this embodiment, the bladder 44 is preloaded with a diluent or other liquid.

In this embodiment, the second micro flow channel 43 communicates with the second chamber 22.

In this embodiment, after the nucleic acid amplification reaction solution flows into the side of the first chamber 21 far away from the fluid quantification pool 5 through the first sample injection assembly 3, the liquid sac 44 is pressed, so that the bottom of the liquid sac 44 contacts the second puncturing structure 41, and the liquid sac 44 is punctured and then flows out of the diluent; when the liquid sac 44 is pressed, air in the microfluidic detection device 100 is extruded, the air enters the first chamber 21 communicated with the second chamber 22 along the second micro-channel 43, nucleic acid amplification reaction liquid in the first chamber 21 is flushed into one side of the first chamber 21 adjacent to the fluid quantifying pond 5 from a bottom gap between the baffle plate 23 and the first chamber 21, and flows into the mixing channel 121, so that pressure sample injection is realized; meanwhile, the diluent enters the second chamber 22 of the functional chamber 2 along the second micro flow channel 43 on the lower surface of the top plate 13 and is far away from the fluid quantifying tank 5 through the second injection hole 42, and along with the increase of the diluent, the diluent flows into the side, close to the fluid quantifying tank 5, of the first chamber 21 from the bottom gap between the baffle plate 23 and the first chamber 21 and fills the side, close to the fluid quantifying tank 5, of the second chamber 22; the diluent and the nucleic acid amplification reaction solution are then mixed in the mixing channel 121, and flow into the fluid quantification reservoir 5, the liquid is slowly chromatographed to the result display module 7 through the capillary suction device 6, and finally the biochemical reaction result is checked through the result display module 7.

In this embodiment, the liquid bags 44 are assembled on the top plate 13, so that the user does not need to prepare liquid such as diluent, the detection steps are reduced, the detection process of the device is totally enclosed, and the pollution of nucleic acid aerosol is avoided.

In the present embodiment, one end of the second micro flow channel 43 near the first communication chamber 221, the second communication chamber 222, and the third communication chamber 223 is connected to the second tributary 45.

As shown in fig. 1, 3, 4 and 5, further, the first sample injection assembly 3 includes a first piercing structure 31, an alignment structure 32, a first sample injection hole 33 and a first micro flow channel 34; the first puncturing structure 31 and the alignment structure 32 are disposed on a surface of the clamping plate 12 away from the accommodating case 11, a positioning groove 321 is disposed on the alignment structure 32, the first puncturing structure 31 is disposed in the alignment structure 32, and the first sample injection hole 33 penetrates through the clamping plate 12 and is disposed in the first puncturing structure 31;

the first micro flow channel 34 is arranged on one surface of the accommodating shell 11 facing the clamping plate 12, or the first micro flow channel 34 is arranged on one surface of the clamping plate 12 facing the accommodating shell 11, and the first micro flow channel 34 is communicated with the first sample injection hole 33 and the functional chamber 2; the top plate 13 is provided with a mounting hole 131, and the first piercing structure 31 and the alignment structure 32 are located in the mounting hole 131.

In this embodiment, the first micro flow channel 34 communicates with the first chamber 21.

In this embodiment, the reagent tube for sample application is aligned with and inserted into the alignment structure 32, for example, the quantitative sample application device 200 with sample to be described later is aligned with and inserted into the alignment structure 32, the puncture structure punctures the reagent tube or the quantitative sample application device 200, the positioning groove 321 provides support and positioning for the reagent tube or the quantitative sample application device 200, and the liquid flows out under pressure and enters the first micro flow channel 34 on the housing case 11 through the first sample injection hole 33 and enters the first chamber 21 of the functional chamber 2 along the first micro flow channel 34, and the liquid and the pre-loaded reagent in the functional chamber 2 perform biochemical reaction, and optionally the liquid is a nucleic acid amplification reaction liquid.

Specifically, the first micro flow channel 34 is disposed on a surface of the accommodating case 11 facing the clamping plate 12.

In this embodiment, one end of the first micro flow channel 34, which is close to the first reaction chamber 211, the second reaction chamber 212 and the waste liquid tank 213, is connected to the first branch flow 35.

In some embodiments, the first and second microchannels 34, 43 have a width of 0.005-50mm and the first and second microchannels 34, 43 have a depth of 0.005-50mm. It will be appreciated that the width of the first micro flow channel 34 and the second micro flow channel 43 may be 0.005mm, 0.05mm, 0.5mm, 5mm, 50mm, or a value between two adjacent values, and likewise, the depth of the first micro flow channel 34 and the second micro flow channel 43 may be 0.005mm, 0.05mm, 0.5mm, 5mm, 50mm, or a value between two adjacent values.

More preferably, the widths of the first micro flow channel 34 and the second micro flow channel 43 are 0.01-10mm, and the depths of the first micro flow channel 34 and the second micro flow channel 43 are 0.01-10mm. It will be appreciated that the width of the first micro flow channel 34 and the second micro flow channel 43 may be 0.01mm, 0.05mm, 0.1mm, 0.5mm, 1mm, 5mm, 10mm, or a value between two adjacent values, and likewise, the depth of the first micro flow channel 34 and the second micro flow channel 43 may be 0.01mm, 0.05mm, 0.1mm, 0.5mm, 1mm, 5mm, 10mm, or a value between two adjacent values.

In some embodiments, the first fluidic channel 34 and the second fluidic channel 43 are machined, laser ablated, 3D printed, injection molded, or chemically etched.

As shown in fig. 2 to 4, further, the result display assembly 7 includes a receiving groove 71, a viewing window 72, and a detecting member (not shown); the accommodating groove 71 is formed in a surface of the clamping plate 12 away from the accommodating shell 11, the detecting member is disposed in the accommodating groove 71, the observation window 72 is formed in the top plate 13 and corresponds to the accommodating groove 71, and one end of the capillary suction member 6 is abutted to the detecting member.

The detection piece comprises chromatographic test paper and/or visible dye detection test paper and/or an optical detector and/or an electric signal detector. Different detection methods can be designed according to requirements, and the nucleic acid detection methods comprise hybridization fluorescence, isothermal amplification, real-time fluorescence PCR, high-resolution melting curve, electrochemical nucleic acid aptamer and the like. It will be appreciated that the corresponding test element may be designed according to the test method, whether the test method or the test apparatus is not limited, as long as the product in which the reagent shows the result after the reaction is suitable for the present application.

In some embodiments, the detection piece comprises a chromatographic test paper, the chromatographic test paper is used for presenting a detection result, a strip-shaped fiber chromatographic material fixed with a detection line and a quality control line is used as a stationary phase, the detection liquid is a mobile phase, a fluorescent labeled antibody or antigen is fixed on a strip-shaped fiber layer, and an analyte is moved and captured on the chromatographic strip through capillary action so as to carry out detection; the chromatographic test paper is pre-installed in the accommodating groove 71, the accommodating groove 71 limits and fixes the chromatographic test paper front, back, left and right, the top plate 13 is matched with the clamping plate 12 to press the upper surface of the chromatographic test paper, and one end of the capillary absorption part 6 is matched with the chromatographic test paper to fix the chromatographic test paper.

In some embodiments, the observation window 72 is located on the upper surface of the top plate 13, and when the microfluidic chip nucleic acid detection method and the device thereof are assembled, the observation window 72 is located above the accommodating groove 71 and corresponds to the chromatographic test paper color development area one by one. When the microfluidic detection device 100 is used for detection, the observation of the detection result can be realized at the observation window 72, and after the reaction is completed, the chromatographic test paper can be discolored, and whether the detection result is negative or positive is judged through the color change.

As shown in fig. 1, 5, 6, and 7, further, the accommodating case 11 includes a first case 111 and a second case 112, and the first case 111 is provided on the second case 112; the functional chamber 2 and the first sample introduction assembly 3 are arranged on the clamping plate 12 and the first shell 111.

Further, a first buckle 1111 and a support column 1112 are disposed on the first housing 111, a buckle groove 1121 is disposed on the second housing 112, the support column 1112 abuts against the inner wall of the second housing 112, and the first buckle 1111 is matched with the buckle groove 1121. The support columns 1112 provide support for the second housing 112 for ease of assembly; the first buckle 1111 and the buckle groove 1121 cooperate to form a dead buckle, and the fixed assembly of the first housing 111 and the second housing 112 is achieved.

As shown in fig. 1, 6 and 7, the microfluidic detection device 100 further includes a heating assembly, a temperature sensor (not shown) and a PCB board (not shown); the heating assembly comprises a heating module 8 and a temperature control module 9, the heating module 8 is arranged on one surface of the first shell 111 facing the second shell 112, the heating module 8 corresponds to the first cavity 21 of the functional cavity 2, the temperature control module 9 and the PCB are arranged on the first shell 111, the temperature sensor is arranged in the first cavity 21 of the functional cavity 2, and the heating assembly is electrically connected with the PCB.

Optionally, the temperature sensor is a ntc probe, the temperature control module 9 supplies power to the heating module 8 through a built-in battery or usb, an internal indicator lamp is turned on after the power is supplied, the heating module 8 starts to work and heat, when the ntc probe senses the temperature required by the reaction, the program enters the heat preservation, and the biochemical reaction of the functional chamber 2 starts to be carried out.

Further, the first housing 111 is provided with a usb interface 1114, and the detection reaction can be controlled by connecting a usb wire or not, or by a power switch.

Further, the first housing 111 is located the position of the functional chamber 2 is provided with a first mounting groove 1113, the second housing 112 is provided with a fixed groove 1122 matched with the first mounting groove 1113, the upper end of the fixed groove 1122 is installed in the first mounting groove 1113, thermal insulation cotton (not shown) is arranged in the fixed groove 1122, the thermal insulation effect of the functional chamber 2 is ensured, the first housing 111 is further provided with a second mounting groove 1117, the second mounting groove 1117 is internally provided with a vibration motor (not shown), the vibration motor is controlled by a PCB (printed circuit board), the motor vibrates according to a certain frequency after being electrified, and the vibration motor can enhance the mixing effect of the reagent.

The first housing 111 is provided with a second buckle 1115 and a positioning structure 1116, and the PCB board is mounted on the first housing 111 through the second buckle 1115 and the positioning structure 1116.

The embodiment of the application also provides a nucleic acid detection apparatus, as shown in fig. 1, which includes a quantitative sample adding device 200 and the microfluidic detection device 100 as described above; the quantitative sample adding device 200 comprises a cylinder 201, a liquid storage tube 205, a tube cover 206 and a piston rod 207; the cylinder 201 comprises a storage cavity 202 and a movable cavity 203 which are mutually communicated, a liquid inlet 204 which is communicated with the movable cavity 203 is formed in the cylinder wall of the cylinder 201, and a liquid storage pipe 205 is arranged at the liquid inlet 204; one end of the piston rod 207 penetrates out of the movable cavity 203, and the other end of the piston rod 207 is used for sealing the liquid inlet 204 and is arranged in the movable cavity 203 in a sliding manner; the tube cover 206 is movably connected with one end of the cylinder 201 away from the piston rod 207, and is used for sealing the storage cavity 202; the tube cover 206 is connected to the first sample injection assembly 3.

The working principle of the quantitative sample adding device 200 provided in the embodiment of the application is as follows: first, the storage chamber 202 is preloaded with a predetermined amount of the first liquid, and the liquid storage tube 205 is preloaded with a predetermined amount of the second liquid; during sampling, the storage cavity 202 is placed upwards, the tube cover 206 is unscrewed, after sampling is completed, the sampled nasal swab or pharyngeal swab is broken off in the liquid storage tube 205, and the sample is stored in the storage cavity 202 preloaded with the first liquid; then the upper tube cap 206 is screwed to seal the storage chamber 202, and the biological reaction is performed with the sample through the first liquid; then, the piston rod 207 is moved in a direction away from the storage chamber 202, the piston rod 207 is made to cancel the sealing of the liquid inlet 204, the quantitative sample loading device 200 is shaken, the second liquid in the liquid storage tube 205 flows out from the liquid inlet 204 and enters the movable chamber 203, if the first liquid is filled in the storage chamber 202, the second liquid is mixed with the first liquid in the storage chamber 202 in the movable chamber 203, if the first liquid is not filled in the storage chamber 202, the second liquid flows into the storage chamber 202 and is mixed with the first liquid in the storage chamber 202, and the second liquid and the first liquid after the biological reaction of the sample are again subjected to the biological reaction, so that the nucleic acid amplification reaction liquid is obtained.

Then, the quantitative sample adding device 200 is connected with the first sample injection assembly 3 of the microfluidic detection device 100, and the first sample injection assembly 3 of the microfluidic detection device 100 penetrates through the tube cover 206 to be communicated with the storage cavity 202; then, the piston rod 207 is moved towards the direction of the storage cavity 202, and the piston rod 207 pushes the nucleic acid amplification reaction liquid in the cylinder 201 to enter the microfluidic detection device 100, so that pressure sample injection of the microfluidic detection device 100 is realized.

The beneficial effect that a nucleic acid detection equipment that this application embodiment provided is: the storage cavity 202 is used for the first liquid and storing the sample, and the sample can be directly placed in the storage cavity 202 after sampling to perform biological reaction, so that the steps of taking out quantitative first liquid from the outside and performing biological reaction on the sample by an operator in the detection process are reduced. The liquid storage tube 205 is integrated at the liquid inlet 204 on the side wall of the barrel 201, the liquid inlet 204 is sealed through the piston rod 207 in an initial state, the second liquid preloaded in the liquid storage tube 205 is prevented from entering the storage cavity 202, when the piston rod 207 moves towards the direction far away from the storage cavity 202, and the sealing of the liquid inlet 204 is canceled, the quantitative sample adding device 200 is shaken, so that the second liquid is mixed with the first liquid after the biological reaction of the sample, and the step of extracting the quantitative second liquid from the outside by an operator in the detection process is reduced again. The piston rod 207 applies pressure to the mixed liquid in the cylinder 201, and the moving distance of the piston rod 207 in the movable cavity 203 towards the storage cavity 202 is controlled to realize quantitative mixed liquid volume, so that the quantitative pressure sample injection of the microfluidic detection device 100 is finally realized.

In the whole sample adding process of the microfluidic detection device 100, the sample adding of the second liquid and the sample adding of the mixed liquid can be realized through the movement of the piston rod 207 without pipetting from the outside for many times, so that complex and huge instrument and equipment are avoided, pipetting with the outside is reduced, the operation is simple, the detection efficiency is improved, the flexibility is improved, special training and learning are not needed, and the hand-operated operation is realized, and the portable detection is realized; the application can accurately control the volume of the sample adding quantity by controlling the moving distance of the piston rod 207 in the movable cavity 203 towards the storage cavity 202, realizes accurate control of sample adding and detection, can add samples to various biological samples, has a very wide application range, is suitable for rapid detection and home self-detection, is sealed in the whole reaction process of the equipment, does not need to uncap for sample adding, and avoids aerosol pollution in the nucleic acid amplification process.

Further, the first liquid preloaded in the storage chamber 202 of the cartridge 201 is a bio-reactive reagent, such as a nucleic acid lysate, and the viral nucleic acid of the sample is released and purified by the first liquid. The second liquid pre-filled in the liquid storage tube 205 is a biological reaction reagent, such as a diluent, and the second liquid dilutes the liquid after the reaction of the first liquid and the viral nucleic acid.

Further, the amount of the first liquid pre-filled in the storage chamber 202 may be between 50u l and 5ml, the size of the first liquid storage amount may be adjusted according to different biological reactions, and the amount of the second liquid pre-filled in the liquid storage tube 205 may be determined according to biological reactions, which is not limited herein.

Further, the tube cover 206 includes a cover body (not shown) and a sealing film (not shown), the middle portion of the cover body, which is far away from one end of the piston rod 207, is hollow, the cover body is in threaded connection or interference fit with the cylinder 201, and the sealing film is disposed in the hollow portion of the cover body.

In this embodiment, when the quantitative sample-adding device 200 is connected to the microfluidic detection device 100, the first sample-injecting component 3 of the microfluidic detection device 100 penetrates through the sealing film and circulates with the storage cavity 202 without removing the tube cover 206 from the cylinder 201, so that sample injection of the microfluidic detection device 100 can be realized, and the operation steps are reduced.

Further, the cover body is annular, an annular connecting groove is formed in the cover body, and the connecting groove is in threaded connection with the end part of the cylinder 201; which is advantageous in improving the sealability of the storage chamber 202 in the cartridge 201.

Further, the sealing film is made of composite aluminum film, rubber or silica gel.

It is apparent that the embodiments described above are only some embodiments of the present application, but not all embodiments, the preferred embodiments of the present application are given in the drawings, but not limiting the patent scope of the present application. This application may be embodied in many different forms, but rather, embodiments are provided in order to provide a more thorough understanding of the present disclosure. Although the present application has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing, or equivalents may be substituted for elements thereof. All equivalent structures made by the specification and the drawings of the application are directly or indirectly applied to other related technical fields, and are also within the protection scope of the application.

Claims

1. A microfluidic detection device, comprising: the device comprises a shell, wherein a functional cavity, a first sample injection assembly, a second sample injection assembly, a fluid quantifying tank, a capillary absorption part and a result display assembly are arranged in the shell;

the first sample injection assembly and the second sample injection assembly are both communicated with the functional cavity, the functional cavity is communicated with the fluid quantifying tank, the fluid quantifying tank is communicated with the result display assembly through the capillary absorption part, and the result display assembly is used for displaying a detection result.

2. The microfluidic detection device of claim 1, wherein the housing comprises a containment shell, a clamping plate and a top plate, the clamping plate being disposed on the containment shell, the top plate being disposed on the clamping plate;

3. The microfluidic detection device of claim 2, wherein the capillary suction attachment comprises a first suction plate and a second suction plate;

the first adsorption plate and the second adsorption plate are arranged on one surface of the top plate, which faces the clamping plate, at intervals, and a gap between the first adsorption plate and the second adsorption plate forms an adsorption groove; one ends of the first adsorption plate and the second adsorption plate extend downwards to the fluid quantitative tank, and the other ends of the first adsorption plate and the second adsorption plate are abutted to the result display assembly.

4. The microfluidic detection device of claim 2, wherein the top plate surface is hydrophilic; alternatively, the surface of the capillary adsorbing piece is hydrophilic.

5. The microfluidic detection device of claim 2, wherein the functional chambers comprise a first chamber and a second chamber, the first chamber being disposed on the containment shell and the second chamber being disposed on the clamping plate;

the first sample injection assembly is arranged in the first cavity and communicated with the second cavity, and the second cavity is communicated with the first cavity and the fluid quantitative tank.

6. The microfluidic detection device of claim 5, wherein the second chamber comprises a first communication chamber, a second communication chamber, and a third communication chamber; the second sample injection assembly is provided with a second tributary close to one end of the first communication cavity, the second communication cavity and the third communication cavity, and the second tributary is corresponding to the first communication cavity, the second communication cavity and the third communication cavity in number and is respectively communicated with the first communication cavity, the second communication cavity and the third communication cavity;

7. The microfluidic detection device according to claim 6, wherein the number of the fluid quantification reservoirs is two, and the two fluid quantification reservoirs are respectively communicated with the first communication cavity and the second communication cavity; and/or the number of the groups of groups,

8. The microfluidic detection device according to claim 5, wherein a baffle is disposed in the second chamber, the baffle extending into the first chamber and being spaced from the bottom of the first chamber; and/or the number of the groups of groups,

9. The microfluidic detection device of any one of claims 2 to 8, wherein the second sample injection assembly comprises a second lancing structure, a second sample injection hole, a second microchannel, and a fluid bladder;

the second puncture structure is arranged on one surface of the top plate away from the clamping plate, the second sample injection hole penetrates through the top plate and is positioned in the second puncture structure, the second micro-flow channel is arranged on one surface of the top plate towards the clamping plate, the second micro-flow channel is communicated with the second sample injection hole and the functional cavity, and the liquid sac is arranged on the top plate and is positioned above the second puncture structure.

10. The microfluidic detection device of any one of claims 2 to 8, wherein the first sample injection assembly comprises a first lancing structure, an alignment structure, a first sample injection hole, and a first microchannel;

the first puncture structure and the alignment structure are arranged on one surface of the clamping plate, which is far away from the accommodating shell, the alignment structure is provided with a positioning groove, the first puncture structure is positioned in the alignment structure, and the first sample injection hole penetrates through the clamping plate and is positioned in the first puncture structure;

the first micro-flow channel is arranged on one surface of the accommodating shell facing the clamping plate, or is arranged on one surface of the clamping plate facing the accommodating shell, and is communicated with the first sample injection hole and the functional cavity;

the top plate is provided with a mounting hole, and the first puncture structure and the alignment structure are positioned in the mounting hole.

11. The microfluidic detection device of any one of claims 2 to 8, wherein the result display assembly comprises a receiving slot, a viewing window, and a detection member;

the holding groove is located splint keep away from the one side of holding shell, the detection spare is located in the holding groove, the observation window is located on the roof and with the holding groove corresponds, the one end of capillary absorption spare with the detection spare butt.

12. The microfluidic detection device according to any one of claims 2 to 8, wherein the housing shell comprises a first housing and a second housing, the first housing being provided on the second housing; the functional chamber and the first sample injection assembly are arranged on the clamping plate and the first shell.

13. The microfluidic detection device of claim 12, further comprising a heating assembly, a temperature sensor, and a PCB board;

the heating assembly comprises a heating module and a temperature control module, the heating module is arranged on one face of the first shell, which faces the second shell, the heating module corresponds to the position of the functional cavity, the temperature control module and the PCB are arranged on the first shell, the temperature sensor is arranged in the functional cavity, and the heating assembly is electrically connected with the PCB.

14. A nucleic acid detection apparatus comprising a quantitative sample addition device and a microfluidic detection device according to any one of claims 1 to 13;

the quantitative sample adding device comprises a cylinder body, a liquid storage pipe, a pipe cover and a piston rod; the cylinder body comprises a storage cavity and a movable cavity which are mutually communicated, the cylinder wall of the cylinder body is provided with a liquid inlet which is communicated with the movable cavity, and the liquid storage pipe is arranged at the liquid inlet; one end of the piston rod penetrates out of the movable cavity, and the other end of the piston rod is used for sealing the liquid inlet and is arranged in the movable cavity in a sliding manner; the tube cover is movably connected with one end of the cylinder body, which is far away from the piston rod, and is used for sealing the storage cavity;

The tube cover is connected with the first sample injection assembly.