CN113495085A - POCT blood cell analyzer and liquid flow driving device of impedance detection cell - Google Patents
POCT blood cell analyzer and liquid flow driving device of impedance detection cell Download PDFInfo
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- CN113495085A CN113495085A CN202010260828.6A CN202010260828A CN113495085A CN 113495085 A CN113495085 A CN 113495085A CN 202010260828 A CN202010260828 A CN 202010260828A CN 113495085 A CN113495085 A CN 113495085A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
- G01N2021/513—Cuvettes for scattering measurements
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a POCT blood cell analyzer and a liquid flowing driving device of an impedance detection pool, wherein the driving device comprises a pressure source; the impedance detection cell comprises a front cell, a rear cell and a detection electrode, wherein the front cell and the rear cell are communicated through a micropore, and the detection electrode is used for matching impedance detection; the pressure source is used to apply pressure to the front cell such that liquid in the front cell flows through the pores to the back cell. The liquid in the front pool flows to the rear pool through the micropores by applying pressure to the front pool, an additional negative pressure generating device is not needed, and the pressure source can reuse a liquid transfer device in the POCT blood cell analyzer, so that the structure of a product assembly can be simplified, and the cost can be reduced.
Description
Technical Field
The invention relates to the technical field of blood sample analysis, in particular to a POCT blood cell analyzer and a liquid flow driving device of an impedance detection cell.
Background
The hemocyte analyzer is called hemocyte analyzer, blood-ball meter, blood-cell counter, etc. and is one of the widely used instruments for clinical examination in hospital.
Most parts of the components in the conventional blood cell analyzer belong to a cleaning system, because the use trace of the previous blood sample must be cleaned before the next blood sample detection. The whole cleaning system is complex in structure and multiple in parts, and a large amount of reagents are needed in the cleaning process, so that the cleaning system occupies a long time.
Compared with the traditional blood cell analyzer, the POCT blood cell analyzer is greatly simplified in instrument components, and the POCT blood cell analyzer can completely remove components related to a cleaning liquid path in the traditional blood analysis, so that the complexity and the production cost of a product are greatly reduced.
Among the current hematology analyzer, its impedance detection pond all is through the negative pressure mode drainage, acts on the mode entering back pond of liquid in the pond with the cell stream promptly through the negative pressure in making the front pool promptly, needs extra negative pressure subassembly through the negative pressure mode drainage, leads to the product subassembly more, and the cost promotes.
Disclosure of Invention
The invention provides a POCT blood cell analyzer and a liquid flow driving device of an impedance detection pool, which can solve the problems of more product components and cost increase caused by additional drainage of a negative pressure component in the prior art.
In order to solve the technical problems, the invention adopts a technical scheme that: providing a drive device for resisting liquid flow of a test cell, the drive device comprising a pressure source;
the impedance detection cell comprises a front cell, a rear cell and a detection electrode, wherein the front cell and the rear cell are communicated through a micropore, and the detection electrode is used for matching impedance detection;
the pressure source is used for applying pressure to the front pool to enable liquid in the front pool to flow to the rear pool through the micropores.
In order to solve the technical problems, the invention adopts a technical scheme that: provided is a POCT blood cell analyzer, including:
a housing;
the detection seat is arranged in the shell and used for receiving the reagent kit to be loaded, the reagent kit comprises an impedance detection pool, the impedance detection pool comprises a front pool and a rear pool which are communicated through micropores and a detection electrode which is used for matching to carry out impedance detection, the detection seat is provided with an impedance detection assembly corresponding to the impedance detection pool, the impedance detection assembly comprises a conductive electrode, and the conductive electrode is electrically contacted with the detection electrode when the reagent kit is loaded into the detection seat;
and the pressure source is arranged in the shell and used for applying pressure to the front pool so that the liquid in the front pool flows to the rear pool through the micropores.
The invention has the beneficial effects that: different from the prior art, the POCT blood cell analyzer and the driving device for liquid flow of the impedance detection pool provided by the invention have the advantages that the liquid in the front pool flows to the rear pool through the micropores by applying pressure to the front pool, an additional negative pressure generating device is not needed, and a pressure source can reuse a liquid transfer device in the POCT blood cell analyzer, so that the structure of a product assembly can be simplified, and the cost is reduced.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the invention, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:
fig. 1 is a schematic perspective view of a POCT blood cell analyzer according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a receiving kit of a POCT blood cell analyzer provided by an embodiment of the present invention;
fig. 3 is a schematic diagram of an internal structure of a POCT blood cell analyzer according to an embodiment of the present invention;
FIG. 4 is a schematic diagram illustrating a separation state of the reagent cartridge and the detection seat according to the embodiment of the present invention;
FIG. 5 is an exploded view of a kit provided by an embodiment of the present invention;
FIG. 6 is a partial cross-sectional view of a kit provided by an embodiment of the present invention;
fig. 7 is a schematic perspective view of a detection seat of a POCT blood cell analyzer according to an embodiment of the present invention;
FIG. 8 is a schematic cross-sectional view of a test socket of the POCT blood cell analyzer according to an embodiment of the present invention;
fig. 9 is a schematic view of an internal structure of a POCT blood cell analyzer according to an embodiment of the present invention;
FIG. 10 is a schematic view of a film tearing mechanism provided by an embodiment of the present invention;
FIG. 11 is a schematic view of another film tearing mechanism provided by embodiments of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive work based on the embodiments of the present invention, are within the scope of the present invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship between the components, the movement situation, and the like in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indications are changed accordingly.
In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Referring to fig. 1 to 11, a POCT blood cell analyzer 10 according to an embodiment of the present invention includes a housing 11, a detection seat 200, a liquid-moving device 300, and a movement mechanism 400.
The housing 11 is provided with a display module 12, a door 13, and a handle 15. The display screen module 12 can be fixedly installed on the upper portion of the side surface of the housing 11, the display screen module 12 can be arranged in an inclined manner so as to be convenient to view, the display screen module 12 can be used for displaying a detection result and performing touch operation, the housing 11 is provided with an opening 14, the door panel 13 is used for covering the opening 14 and can be rotatably connected with the lower portion or the side portion of the housing 11, and the handle portion 15 can be arranged on the top portion of the housing 11 so as to facilitate the portable movement of the POCT blood cell analyzer 10. Of course, the display screen module 12 may also be eliminated, the main control module in the POCT blood cell analyzer 10 is connected to a mobile communication device such as a mobile phone or a tablet computer through a communication method such as bluetooth connection, and the detection result and the corresponding touch operation are displayed through the mobile phone or the tablet computer.
The detection seat 200 is disposed on the casing 11 for receiving the reagent cartridge 100, the detection seat 200 is provided with a plurality of impedance detection components 210 and/or a plurality of optical detection components 220, when the door 13 is opened relative to the casing 11, the detection seat 200 can be moved out of the casing 11 through a sliding component or a hinge component inside the casing 11 to receive the reagent cartridge 100, the impedance detection components 210 have electrode conductive devices 211 (see fig. 8), the electrode conductive devices 211 may specifically be elastic ejector pins (pogo pins), and the electrode conductive devices 211 are electrically connected with the impedance detection cells 120 of the reagent cartridge 100 after the reagent cartridge 100 is installed in the detection seat 200. The detection principle of using the impedance detection module 210 and the optical detection module 220 to perform impedance detection and optical detection on blood cells does not relate to the invention, and is not described in detail in this application. Each impedance detection element 210 and each optical detection element 220 can detect a separate item to obtain a specific blood cell parameter value.
The pipette 300 is disposed in the housing 11 and can be located above the detection seat 200, and is used for sleeving a pipette head 114 (such as a tip head) to perform a pipetting operation on the kit 100, wherein the pipetting operation refers to a liquid suction, moving and liquid discharge operation, and the pipette 300 can also be used for repeatedly sucking and discharging liquid to form a sucking, discharging and uniformly mixing effect, unlike a conventional sampling needle, a sample can enter the inside of the sampling needle during the pipetting operation of the conventional sampling needle, so that the inner wall and the outer wall of the sampling needle can be polluted, a cleaning operation is required before each detection, the pipette head 114 is sleeved on the pipette 300 provided by the present invention, and the pipette head 114 has a certain volume, the sample does not enter the interior of the pipette 300 during the pipetting operation, does not contaminate the pipette 300, therefore, the liquid transfer device 300 does not need to be cleaned, complex cleaning components and cleaning processes are omitted, and the detection efficiency is improved.
The pipette 300 of the present invention may include a motor, a cavity, and a piston, wherein the motor is used to drive the piston to reciprocate in the cavity to perform a pipetting operation.
The pipette 300 of the present invention may be an ADP (Air Driven Pump), which is a power device with precise volume and capable of generating positive and negative pressure.
The traditional blood cell analyzer adopts a set of complex devices such as an air pump, an air valve, a pressure chamber and the like to realize liquid preparation, counting and cleaning. The POCT blood cell analyzer provided by the application finishes liquid preparation and counting by ADP and does not need cleaning. In addition, the ADP is a modular component, the structure is flexible, the ADP is accurately driven by a motor, and the ADP is slightly interfered by other systems.
The pipette 300 may also be used to bubble the liquid in the kit 100 for the mixing operation, and specifically, when the pipette 300 performs the mixing operation by bubbling, the pipette 300 sucks air above the liquid surface and discharges air below the liquid surface. Of course, also can adopt independent air pressure system to the bottom input air of cell body, corresponding increase extra air pressure system subassembly can, pipettor 300 is used for moving the liquid promptly in this application, still can be used to carry out the bubble mixing, and the subassembly is very simplified.
The movement mechanism 400 is used to move the pipette 300 and the detection seat 200 relative to each other, and the movement mechanism 400 may be a three-axis movement system to drive the pipette 300 to displace in the direction X, Y, Z, of course, the implementation manner of the movement mechanism 400 is not limited thereto, and the movement mechanism 400 may also drive the detection seat 200 to displace relative to the pipette 300 or drive the pipette 300 and the detection seat 200 to displace simultaneously.
In one embodiment, the moving mechanism includes a first motor transmission component 410, a second motor transmission component 420, and a third motor transmission component 430, wherein the first motor transmission component 410 is used for driving the detection seat to move in the X direction; the second motor transmission assembly 420 is used for driving the pipettor to move in the Y direction; the third motor transmission assembly 430 is used for driving the pipettor to move in the Z direction, the first motor transmission assembly 410, the second motor transmission assembly 420, and the third motor transmission assembly 430 may all include a motor 401, a transmission wheel 402, a transmission belt 403, and a transmission member 404, the motor 401 and the transmission wheel 402 are arranged at an interval, the transmission belt 403 is sleeved on the output end of the motor 401 and the transmission wheel 402, the transmission member 404 is fixed with the transmission belt 403 and is in transmission fit with the detection seat 200 or the ADP, for example, the transmission member 404 is provided with a U-shaped bayonet, and the detection seat 200 and the ADP are slidably arranged through a slider and a slide rail mechanism and receive a driving force transmitted from the U-shaped bayonet to perform corresponding sliding.
According to the POCT blood cell analyzer provided by the embodiment of the invention, the kit 100 integrates the necessary accessories (the impedance detection cell 120 and the optical detection cell 130) of the test system and various reagents, and the kit 100 is disposable, so that a huge cleaning system can be eliminated, and the number of the parts of the whole analyzer is greatly reduced, wherein the impedance detection cell 120 and the optical detection cell 130 are main cleaning objects in the traditional blood cell analyzer.
Referring to fig. 4 to 6, the embodiment of the invention provides a kit 100, where the kit 100 may be a disposable kit or a reusable kit, and the kit 100 includes a kit body 110, a plurality of impedance detection cells 120, a plurality of optical detection cells 130, a reagent cell 140, a kit bottom 150, and a sealing layer 160. The kit 100 has an asymmetric structure, and is convenient to be placed in the detection seat 200 in a foolproof manner.
In one embodiment, the cassette body 110 may be rectangular, and the cassette body 110 may have a first insertion hole 111, where the first insertion hole 111 is used to receive a sample tube 113; alternatively, the cartridge 110 may be directly connected to a sample cell, such as an integral connection or a sleeve connection, and the sample tube 113 may be used to receive a sample, such as a blood sample. The cassette 110 may further be provided with a second insertion hole 112, a first insertion hole 111 for receiving a pipette tip 114, the pipette tip 114 being adapted to be mounted on a pipette 300 for cooperating pipetting operations.
A plurality of impedance detection cells 120 are connected to cartridge 110 for matching impedance detection, and the number of impedance detection cells 120 is at least one, and two are shown in fig. 5.
A plurality of optical detection cells 130 are connected to the box body 110 for performing photoelectric detection, and the number of the optical detection cells 130 is at least one, and two are shown in fig. 5.
The reagent reservoir 140 is connected to the cartridge body 110 for accommodating reagents, such as a hemolytic agent, an antibody reagent, a diluent, and the like. The reagent reservoir 140 may be provided in plural so as to accommodate plural kinds of reagents, and of course, one reagent reservoir 140 may be provided.
The box body 110 is arranged at the opening end of the box bottom 150, and the impedance detection pool 120 and the reagent pool 140 are contained in the box bottom 150; the optical detection cell 130 extends out of the cassette bottom 150; the impedance measuring cell 120, the optical measuring cell 130, and the reagent cell 140 each have an opening 115 communicating with the cartridge 110, and the sealing layer 160 seals and covers the opening 115 (see fig. 10 and 11).
In a specific embodiment, the opening 115 corresponding to the optical detection cell 130 may be a rectangular hole 115a, the opening 115 corresponding to the reagent cell 140 may be a kidney-shaped hole, and includes a large number of small kidney-shaped holes 115b and a large kidney-shaped hole 115c, the reagent cell 140 corresponding to the small kidney-shaped hole 115b may be used to install a plurality of special reagents, such as hemolytic agents and antibody reagents, the reagent cell 140 corresponding to the large kidney-shaped hole 115c may be used to install a diluent and serve as a mixing cell, receive a blood sample and other reagent inputs to support dilution and mixing, the opening 115 corresponding to the impedance detection cell 120 is special, and is a small kidney-shaped hole 115d, the existing impedance detection cell 120 is drained by a negative pressure mode, that is, a liquid in the front cell enters the rear cell by a cell flow mode through the negative pressure acting on the rear cell, an additional negative pressure component is needed for draining by the negative pressure mode, and the application directly uses a 300 pipette to provide positive pressure to the opening 115 of the small kidney-shaped hole 115d so that the front cell provides positive pressure to the front cell The liquid in the chamber 121 enters the rear pool 122 in a cell flow mode, so that negative pressure components can be reduced, and the equipment structure is simplified.
The impedance detection pool 120 is a group or a plurality of groups, the impedance detection pool 120 includes a front pool 121, a rear pool 122 and a detection device 123 for matching impedance detection, the detection device 123 can be an electrode plate, the box bottom 150 is provided with an exposed hole corresponding to the detection device 123 so as to realize that the detection device 123 is electrically connected with an electrode conductive device 211 (see fig. 8) of the impedance detection assembly 210, the electrode conductive device can be an elastic thimble (pogo pin), the impedance detection pool 120 can further include a waste liquid cavity (not shown) communicated with the rear pool 122 for receiving waste liquid, and after positive pressure is provided so that liquid in the front pool 121 enters the rear pool 122 in a cell flow manner, the waste liquid is finally collected by the waste liquid cavity, thereby avoiding waste liquid flowing and scattering.
The optical detection cells 130 are one or more and are adapted to perform a transmitted light photodetection and/or a scattered light photodetection.
The impedance detection cell 120, the optical detection cell 130, and the reagent cell 140 may be integrally formed with the cartridge body 110; or the cartridge body 110 is integrally formed with the assembly cartridge 116, and at least some of the impedance detection cell 120, the optical detection cell 130, and the reagent cell 140 are connected with the assembly cartridge 116 in an abutting manner, such as a snap connection or an interference fit.
The optical detection cell 130 may be made of optical plastic, transparent plastic, or glass.
In one embodiment, the reagent kit 100 is provided with a plurality of cell bodies, which may be arranged in a single row, and correspondingly, the motion mechanism may be a two-dimensional motion mechanism, and drives the ADP to perform reciprocating motion and lifting motion on a two-dimensional plane.
In another embodiment, the reagent kit is provided with a plurality of cell bodies which are arranged in a plurality of rows, and the movement mechanism is a three-dimensional movement mechanism which drives ADP to perform three-dimensional movement in a three-dimensional space.
The kit 100 provided by the invention can support impedance detection and/or colorimetric detection and/or turbidimetric detection on a blood sample by arranging the plurality of impedance detection cells 120, the plurality of optical detection cells 130 and the plurality of reagent cells 140, each optical detection cell 130 and each reagent cell 140 can be matched for detecting an independent item to obtain a specific blood cell parameter value, the detection items are more, the expandability of the detection items is good, the dilution of a sample to be detected and the pretreatment of adding a reagent can be allocated by the reagent pre-filled in the reagent cell 140, and the POCT detection efficiency is optimized. In addition, the kit 100 itself can be used as a waste liquid collector, so that a reagent barrel and a waste liquid barrel which are required to be connected with a traditional blood cell analyzer are omitted, and the POCT blood cell analyzer 10 can be portable and movable.
Referring to fig. 1 to 11 together, an embodiment of the POCT blood cell analyzer 10 includes a housing 11, a bracket 16, a detection seat 200, a pipette 300, a movement mechanism 400, a battery module 500, a power supply module, a temperature control module, and a film tearing mechanism.
The housing 11 is provided with a display module 12, a door 13, and a handle 15. The display screen module 12 can be fixedly installed on the upper portion of the side surface of the casing 11, the display screen module 12 can be obliquely arranged so as to be convenient to view, the display screen module 12 can be used for displaying a detection result and performing touch operation, the casing 11 is provided with an opening 14, the door panel 13 is used for covering the opening 14 and can be rotatably connected with the lower portion or the side portion of the casing 11, the detection seat 200 can be moved out of the casing 11 and receive the reagent kit 100 to be loaded when the door panel 13 is opened, and the handle portion 15 can be arranged on the top of the casing 11 so as to facilitate the portable movement of the POCT blood cell analyzer 10. Of course, the display screen module 12 may also be eliminated, the main control module in the POCT blood cell analyzer 10 is connected to a mobile communication device such as a mobile phone or a tablet computer through a communication method such as bluetooth connection, and the detection result and the corresponding touch operation are displayed through the mobile phone or the tablet computer.
The detection seat 200 is arranged in the shell 11 and is used for receiving the kit 100 to be loaded, wherein the detection seat 200 is arranged in a sliding manner relative to the shell 11, and the detection seat 200 is used for receiving the kit 100 to be loaded when sliding out of the shell 11; alternatively, the test socket 200 is hinged with respect to the housing 11, and the test socket 200 is used for receiving the reagent cartridge 100 when being screwed out of the housing 11.
The detection seat 200 is provided with a plurality of impedance detection components 210 and/or a plurality of optical detection components 220, the impedance detection components 210 include electrode conductive devices 211 (see fig. 8), the electrode conductive devices 211 can be elastic ejector pins (pogo pins), the reagent kit 100 is provided with detection devices 123, the detection devices 123 can be electrode plates, the electrode conductive devices 211 and the detection devices 123 are in electrical contact when the reagent kit 100 is installed in the detection seat 200, and the optical detection components 220 include light emitting components and light receiving components which are arranged at intervals. The detection principle of detecting blood cells by using the impedance detection module 210 and the optical detection module 220 does not relate to the invention, and is not described in detail in this application.
The pipette 300 is disposed in the housing 11 and above the detection seat 200, and is used for sleeving a pipette head 114 (such as a tip head) to perform a pipetting operation on the kit 100. The pipette 300 of the present invention may include a motor, a cavity, and a piston, wherein the motor is used to drive the piston to reciprocate in the cavity to perform a pipetting operation. The pipette 300 of the present invention may be an ADP (Air Driven Pump), which is a power device with precise volume and capable of generating positive and negative pressure. The traditional blood cell analyzer adopts a set of complex devices such as an air pump, an air valve, a pressure chamber and the like to realize liquid preparation, counting and cleaning. The POCT blood cell analyzer provided by the application finishes liquid preparation and counting by ADP and does not need cleaning. In addition, the ADP is a modular component, the structure is flexible, the ADP is accurately driven by a motor, and the ADP is slightly interfered by other systems.
A carriage 16 is provided within the housing 11 and the ADP is mounted on the carriage 16 by a movement mechanism 400.
Wherein, move liquid operation and refer to imbibition, remove, spit liquid operation, include specifically:
dispensing the blood sample to a specific reagent reservoir 140, which may be pre-filled with a diluent, the specific reagent reservoir 140 may be a reagent reservoir 140 having a large kidney-shaped aperture 115 c;
adding the reagents in the other reagent pools 140 into the specific reagent pool 140 to mix so as to form a sample to be detected, wherein the other reagent pools 140 can be reagent pools 140 with small waist-shaped holes 115 b;
the sample to be detected is moved into the impedance detection cell 120 and/or the optical detection cell 130 for impedance detection and/or optical detection.
The movement mechanism 400 is disposed in the housing 11, and is used for moving the pipette 300 relative to the detection seat 200 to support a pipetting operation, and the movement mechanism 400 may be a three-axis movement system to drive the pipette 300 to displace in the direction X, Y, Z, of course, the implementation manner of the movement mechanism 400 is not limited thereto, and the movement mechanism 400 may also drive the detection seat 200 to displace relative to the pipette 300 or drive the pipette 300 and the detection seat 200 to displace simultaneously.
The battery module 500 is electrically connected to the test socket 200, the movement mechanism 400, and the pipette 300, and the POCT blood cell analyzer 10 can be conveniently used in various places, for example, outdoors, by providing the battery module 500. In order to facilitate the assembly and disassembly of the battery module 500, a battery cavity may be disposed on the outer surface of the housing 11, and the battery module 500 and the battery cavity are assembled by a snap fit, or the POCT blood cell analyzer is provided with a battery cavity and a cover plate, the battery module and the battery cavity are assembled by a snap fit and are covered by the cover plate, the cover plate may be an instrument housing, or may be a panel on any side surface or top end of the instrument, and the two ways may both achieve the convenient assembly and disassembly of the battery module 500. Certainly, the POCT blood cell analyzer 10 further includes a power supply module electrically connected to the detection seat 200, the movement mechanism 400 and the pipette 300, and configured to connect to a mains supply.
The temperature control module is attached to the detection base 200 to provide a detection temperature. As shown in fig. 7, the temperature control module may be a heating film 510 attached to the bottom surface or the side surface of the detection seat 200, or the temperature control module may be a heating rod, and accordingly, the detection seat 200 is provided with a mounting hole for receiving the heating rod; the temperature control module can also be a peltier 520, and the peltier 520 is used for heating or cooling the heating base. The POCT blood cell analyzer provided by the invention can independently heat or refrigerate by arranging the temperature control module, and the environmental temperature has little influence on detection.
In one embodiment, the pipette 300 includes a motor, a cavity, and a piston, wherein the motor is used to drive the piston to reciprocate in the cavity to perform pipetting operations.
The film tearing mechanism is used for tearing the sealing layer 160 on the surface of the kit 100; or a peel mechanism for puncturing the sealing layer 160 on the surface of the kit 100.
As shown in fig. 10, the film tearing mechanism may be a fixedly arranged hook 610, the hook 610 is used for hooking the tab portion 162 of the sealing layer 160 when the detection seat 200 receives the reagent kit 100 and enters the housing 11, so as to realize automatic film tearing, the hook 610 may be in an L-shaped plate, and the tab portion 162 is provided with a corresponding hook hole. If a manual film tearing mode is adopted, namely the reagent kit 100 is arranged in the detection seat 200 after the sealing layer 160 is torn off, due to the uncertainty of operation, the reagent pre-arranged in the reagent kit 100 is easily spilled, so that the normal detection is influenced due to the insufficient amount of the reagent.
As shown in fig. 11, in another embodiment, the film tearing mechanism includes a plate 621, a film breaking column 622 disposed on one surface of the plate 621, and a driving mechanism 623 for driving the plate 621 to ascend and descend, the film breaking column 621 corresponds to the cell body of the reagent kit 100, the driving mechanism 623 is configured to drive the plate 621 so that the film breaking column 622 pierces the sealing layer 160 on the surface of the reagent kit 100, a free end of the film breaking column 622 has a gradually decreasing cross-sectional area, the film breaking column 622 may be in a conical shape, and the driving mechanism 623 may be a motor and a transmission belt or a motor and gear transmission mechanism.
The embodiment of the present invention further provides a detection method based on the POCT blood cell analyzer 10, where the method includes:
receiving the reagent kit 100 and putting the reagent kit 100 into the detection seat 200, wherein the reagent kit 100 comprises a plurality of impedance detection cells 120 and/or a plurality of optical detection cells 130 which are used for matching impedance and/or photoelectric detection, the reagent kit 100 further comprises a plurality of reagent cells 140, the reagent cells 140 are used for containing a plurality of reagents, the reagent kit 100 is further provided with a sample tube, and a blood sample is filled in the sample tube;
dispensing a blood sample into a specific reagent reservoir 140 by a pipette 300, i.e. automatic blood dispensing by a device;
adding the reagents in other reagent pools 140 into the specific reagent pool 140 through the pipettor 300 for mixing to form a sample to be detected, namely, automatically adding the reagents through equipment;
the sample to be detected is moved into the impedance detection cell 120 and/or the optical detection cell 130 by the pipette 300 to perform impedance detection and/or optical detection.
The POCT blood cell analyzer and the detection method provided by the invention can realize automatic distribution of blood samples and automatic reagent addition through the matching of the motion mechanism and the pipettor, thereby optimizing the POCT detection efficiency. In the existing detection method, reagents are required to be added manually, and the reagents are poured into an instrument for detection after being mixed manually.
Wherein, the step of receiving the reagent kit 100 into the test seat 200 comprises the following steps:
tearing the sealing layer 160 on the surface of the kit 100 by using a film tearing mechanism; or a tear mechanism may be used to puncture the sealing layer 160 on the surface of the kit 100.
Wherein the step of dispensing the blood sample into the specific reagent reservoir 140 by the pipette 300 is preceded by:
the pipette 300 is inserted and butted with a pipette tip 114 preset on the kit 100, so that the blood sample, the reagent and the sample to be detected are contacted only through the pipette tip 114 when the pipette 300 performs the pipetting operation, and the pipette 300 is prevented from being polluted.
Wherein, utilize pipettor 300 to provide the malleation in order to impel the sample that awaits measuring in the impedance detection pond to flow when carrying out the impedance detection, can reduce among the prior art need extra negative pressure device to carry out the impedance detection drainage through multiplexing pipettor 300, reduced the product subassembly.
According to the POCT blood cell analyzer and the detection method provided by the embodiment, automatic blood sample distribution and automatic reagent addition can be realized through the cooperation of the movement mechanism and the pipettor, and the POCT detection efficiency is optimized.
The embodiment of the present invention further provides a liquid flow driving device for the impedance detection cell 120, the driving device includes a pressure source, the impedance detection cell 120 includes a front cell 121, a rear cell 122 and a detection device 123 for performing impedance detection in cooperation, the pressure source is configured to apply pressure to the front cell 121 so that the liquid in the front cell 121 flows to the rear cell 122 through the micro-hole.
The pressure source may be embodied as a pipette 300, the pipette 300 being configured for pipetting when the pipette head 114 is docked and the pipette 300 being further configured for applying pressure when the pipette head 114 is undocked. The pipette 300 of the present invention may be an ADP (Air Driven Pump), which is a power device with precise volume and capable of generating positive and negative pressure. The traditional blood cell analyzer adopts a set of complex devices such as an air pump, an air valve, a pressure chamber and the like to realize liquid preparation, counting and cleaning. The POCT blood cell analyzer provided by the application finishes liquid preparation and counting by ADP and does not need cleaning. In addition, the ADP is a modular component, and has a flexible architecture and is less interfered by other systems.
The pipette 300 includes a motor, a cavity, and a piston, and the motor is used to drive the piston to reciprocate in the cavity to perform pipetting operations.
The top end of forebay 121 is provided with an opening 115, and opening 115 is used for close-fitting docking with the free end of pipette 300 to receive pressure input into forebay 121. The opening 115 may be in the shape of a small circular hole 115d to facilitate a tight fit with the free end of the pipette 300.
The embodiment of the invention also provides a POCT blood cell analyzer, which comprises a shell 11, a detection seat 200, a pressure source, a motion mechanism 400, a temperature control module, a storage battery module 50 and a power supply module.
The housing 11 is provided with a display module 12, a door 13, and a handle 15. The display screen module 12 can be fixedly installed on the upper portion of the side surface of the casing 11, the display screen module 12 can be obliquely arranged so as to be convenient to view, the display screen module 12 can be used for displaying a detection result and performing touch operation, the casing 11 is provided with an opening 14, the door panel 13 is used for covering the opening 14 and can be rotatably connected with the lower portion of the casing 11, the detection seat 200 can be moved out of the casing 11 and receive the reagent kit 100 to be loaded when the door panel 13 is opened, and the lifting handle 15 can be arranged on the top of the casing 11 so as to facilitate the portable movement of the POCT blood cell analyzer 10. Of course, the display screen module 12 may also be eliminated, the main control module in the POCT blood cell analyzer 10 is connected to a mobile communication device such as a mobile phone or a tablet computer through a communication method such as bluetooth connection, and the detection result and the corresponding touch operation are displayed through the mobile phone or the tablet computer.
The detection seat 200 is arranged in the shell 11 and used for receiving the reagent kit 100, the reagent kit 100 comprises an impedance detection cell 120, the impedance detection cell 120 comprises a front cell 121 and a rear cell 122 which are communicated through micropores and a detection device 123 used for matching to perform impedance detection, the detection seat 200 is provided with an impedance detection assembly 210 corresponding to the impedance detection cell 120, the impedance detection assembly 210 comprises an electrode conductive device 211, and the electrode conductive device 211 is electrically contacted with the detection device 123 when the reagent kit 100 is arranged in the detection seat 200.
A pressure source is disposed within the housing 11 for applying pressure to the front reservoir 121 to cause liquid in the front reservoir 121 to flow through the pores to the back reservoir 122. The pressure source may be embodied as a pipette 300, the pipette 300 being configured for pipetting when the pipette head 114 is docked and the pipette 300 being further configured for applying pressure when the pipette head 114 is undocked. The pipette 300 of the present invention may be an ADP (Air Driven Pump), which is a power device with precise volume and capable of generating positive and negative pressure. The traditional blood cell analyzer adopts a set of complex devices such as an air pump, an air valve, a pressure chamber and the like to realize liquid preparation, counting and cleaning. The POCT blood cell analyzer provided by the application finishes liquid preparation and counting by ADP and does not need cleaning. In addition, the ADP is a modular component, and has a flexible architecture and is less interfered by other systems.
The liquid transfer device 300 comprises a motor, a cavity and a piston, wherein the motor is used for driving the piston to reciprocate in the cavity so as to perform liquid transfer operation, sucking and spitting mixing operation or inflating and mixing.
As shown in fig. 5, the top end of forebay 121 is provided with an opening 115, and opening 115 is adapted to mate with the free end of pipette 300 to receive pressure input into forebay 121. The opening 115 may be in the shape of a small circular hole 115d to facilitate a tight fit with the free end of the pipette 300.
In particular embodiments, the pipette 300 may include a first syringe and a second syringe slidably nested, with the free end of the second syringe retracted into the free end of the first syringe such that the free end of the first syringe is exposed and thereby received by the pipette head 114, and the second syringe separated from the pipette head 114 when moved toward the free end of the first syringe.
The movement mechanism 400 is disposed in the housing 11, and is used for moving the pipette 300 relative to the detection seat 200 to perform a pipette holding operation; the temperature control module is attached to the detection seat 200 to provide a detection temperature; the battery module 500 is electrically connected to the detection holder 200, the movement mechanism 400, and the pipette 300; and/or the power supply module is electrically connected with the detection seat 200, the movement mechanism 400 and the pipette 300, and is used for connecting with the mains supply.
According to the POCT blood cell analyzer and the driving device for liquid flow of the impedance detection pool, provided by the embodiment of the invention, pressure is applied to the front pool, so that liquid in the front pool flows to the rear pool through the micropores, an additional negative pressure generating device is not needed, and the structure of a product assembly can be simplified.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. A liquid flow driving device for an impedance detection cell, comprising:
the drive device comprises a pressure source;
the impedance detection cell comprises a front cell, a rear cell and a detection electrode, wherein the front cell and the rear cell are communicated through a micropore, and the detection electrode is used for matching impedance detection;
the pressure source is used for applying pressure to the front pool to enable liquid in the front pool to flow to the rear pool through the micropores.
2. The drive device according to claim 1, characterized in that: the pressure source is a liquid transfer device, and the liquid transfer device is also used for carrying out the pressure applying operation when the pipette head is not sleeved.
3. The drive device according to claim 2, characterized in that: the pipettor comprises a motor, a cavity and a piston, wherein the motor is used for driving the piston to reciprocate in the cavity so as to perform pipetting operation.
4. The drive device according to claim 3, characterized in that: the top end of the forebay is provided with an opening part, and the opening part is used for being in tight fit butt joint with the injector to receive pressure input into the forebay.
5. A POCT blood cell analyzer, comprising:
a housing;
the detection seat is arranged in the shell and used for receiving the reagent kit to be loaded, the reagent kit comprises an impedance detection pool, the impedance detection pool comprises a front pool and a rear pool which are communicated through micropores and a detection electrode which is used for matching to carry out impedance detection, the detection seat is provided with an impedance detection assembly corresponding to the impedance detection pool, the impedance detection assembly comprises a conductive electrode, and the conductive electrode is electrically contacted with the detection electrode when the reagent kit is loaded into the detection seat;
and the pressure source is arranged in the shell and used for applying pressure to the front pool so that the liquid in the front pool flows to the rear pool through the micropores.
6. The POCT blood cell analyzer of claim 5, wherein: the pressure source is a liquid transfer device, the liquid transfer device is used for carrying out liquid transfer operation when the pipette head is sleeved, and the liquid transfer device is also used for carrying out pressure application operation when the pipette head is not sleeved.
7. The POCT hematology analyzer of claim 6, wherein: the pipettor comprises a motor, a cavity and a piston, wherein the motor is used for driving the piston to reciprocate in the cavity so as to perform pipetting operation.
8. The POCT hematology analyzer of claim 7, wherein: the top end of the forebay is provided with an opening part, and the opening part is used for being in tight fit butt joint with the injector to receive pressure input into the forebay.
9. The POCT hematology analyzer of claim 6, wherein: the pipettor comprises a first needle cylinder and a second needle cylinder which are arranged in a sliding and nesting mode, the free end of the second needle cylinder retracts into the free end of the first needle cylinder, so that the free end of the first needle cylinder is exposed and then sleeved with the pipette head, and the second needle cylinder moves towards the free end of the first needle cylinder and separates the pipette head.
10. The POCT hematology analyzer of claim 6, further comprising:
the movement mechanism is arranged in the shell and used for enabling the pipettor to move relative to the detection seat so as to carry out the pipetting operation;
the temperature control module is attached to the detection seat to provide detection temperature;
a battery module electrically connected with the detection seat, the movement mechanism, and the pipette; and/or
And the power supply module is electrically connected with the detection seat, the movement mechanism and the pipettor and is used for connecting a mains supply.
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