CN115155687B - Quantitative sample adding device and microfluidic detection equipment - Google Patents
Quantitative sample adding device and microfluidic detection equipment Download PDFInfo
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- CN115155687B CN115155687B CN202211081384.5A CN202211081384A CN115155687B CN 115155687 B CN115155687 B CN 115155687B CN 202211081384 A CN202211081384 A CN 202211081384A CN 115155687 B CN115155687 B CN 115155687B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
Abstract
The embodiment of the application belongs to the technical field of medical treatment, and relates to a quantitative sample adding device and microfluidic detection equipment. The quantitative sample adding device comprises: the device comprises a cylinder, a liquid storage pipe, a sealing plug and a piston rod; the cylinder body comprises a storage cavity and a movable cavity which are mutually communicated, a liquid inlet communicated with the movable cavity is formed in the cylinder wall of the cylinder body, and the liquid storage pipe is arranged at the liquid inlet; one end of the piston rod penetrates through the movable cavity, and the other end of the piston rod is used for sealing the liquid inlet and is arranged in the movable cavity in a sliding manner; the sealing plug is movably connected with one end of the cylinder body, which is far away from the piston rod, and is used for sealing the storage cavity. In the sample adding process of the microfluidic chip, the liquid is not required to be transferred from the outside for multiple times, the sample adding of the second liquid and the sample adding of the mixed liquid can be realized through the movement of the piston rod, the operation is simple, the detection efficiency is high, the flexibility is high, and the portable detection is realized.
Description
Technical Field
The application relates to the technical field of medical treatment, more specifically relates to a quantitative sample adding device and micro-fluidic detection equipment.
Background
Microfluidic chips, also known as Lab-on-a-chips, refer to a biological or chemical laboratory built on a few square centimeters Chip. It integrates the basic operation units of reaction, separation, culture, sorting, detection, etc. involved in the biological and chemical fields into a very small chip, and the network is formed by micro-channels, so that the controllable fluid can penetrate through the whole system to realize various functions of conventional biological or chemical laboratories. The micro-fluidic chip technology has the characteristics of small sample volume, high integration level, easy realization of automatic control, high-flux analysis and the like, so that the biochemical reaction operation on the micro-fluidic chip is more convenient and rapid than the conventional analysis sample pretreatment, and the cost is low. The microfluidic technology can realize the complex operation steps of reagent reaction, separation, detection and the like on one chip, so that the sample processing time is greatly shortened, the cost of reagents and instruments is greatly reduced, and the detection resolution and sensitivity are obviously improved. The volume of the detection device is greatly reduced, portability and field detection are possible, and automation and portability of biochemical detection can be realized.
With the progress of science and technology and the outbreak of new crown epidemic situation, a plurality of full-automatic and biological detection integrated POCT (point-of-care testing, chinese name: instant examination) detection instruments and detection kits appear in recent years, and the microfluidic chip card boxes developed by a plurality of companies at present need corresponding instruments to provide power for chips to achieve the purpose of controlling fluid, such as a mechanical pump, a constant pressure pump, a constant flow pump and the like to provide external power for controlling various liquid flows of the chips, so that the control of complex biochemical reactions is realized.
The microfluidic chip is controlled by a large instrument, the whole process needs complex equipment, is large in size, low in detection efficiency, poor in flexibility, expensive in instrument equipment, complex in whole operation process, needs operators with high professional levels to operate, needs skilled technicians to operate in detection, and cannot realize portable detection.
Disclosure of Invention
The embodiment of the application provides a quantitative sample adding device and a micro-fluidic detection device, and is used for solving the problems that in the prior art, instruments for providing power for a micro-fluidic chip are complex, large in size and complex in operation process, and need operators with high professional levels to operate, so that the detection efficiency is low, the flexibility is poor, and portable detection cannot be realized.
In order to solve the above technical problem, an embodiment of the present application provides a quantitative sample adding device, which adopts the following technical scheme:
a quantitative sample adding device comprising: the device comprises a cylinder, a liquid storage pipe, a sealing plug and a piston rod; the cylinder body comprises a storage cavity and a movable cavity which are mutually communicated, a liquid inlet communicated with the movable cavity is formed in the cylinder wall of the cylinder body, and the liquid storage pipe is arranged at the liquid inlet; one end of the piston rod penetrates through the movable cavity, and the other end of the piston rod is used for sealing the liquid inlet and is arranged in the movable cavity in a sliding manner; the sealing plug is movably connected with one end of the cylinder body, which is far away from the piston rod, and is used for sealing the storage cavity; the storage cavity is used for pre-filling a first liquid and storing a sample, the liquid storage tube is used for pre-filling a second liquid, and when the piston rod moves towards the direction far away from the storage cavity, the sealing of the liquid inlet is cancelled, so that the second liquid flows into the movable cavity, and the second liquid is mixed with the first liquid.
Furthermore, the quantitative sample adding device further comprises a first limiting part protruding out of the inner wall of the movable cavity, the position of the liquid inlet is located between the first limiting part and the storage cavity, the outer wall of the piston rod is provided with a limiting groove, and the first limiting part is abutted to the limiting groove.
Furthermore, the barrel wall of the barrel body is provided with at least two mounting grooves communicated with the movable cavity, the liquid inlet is positioned between any mounting groove and the storage cavity, any mounting groove is opposite to the limiting groove, and the first limiting part penetrates through one mounting groove and then protrudes out of the inner wall of the barrel body.
Furthermore, the quantitative sample adding device further comprises a second limiting part protruding out of the inner wall of the cylinder, the second limiting part is located between the storage cavity and the movable cavity, and one end of the piston rod close to the storage cavity is abutted to the second limiting part.
Furthermore, the quantitative sample adding device further comprises a third limiting part, wherein the third limiting part is sleeved at one end of the piston rod, which penetrates out of the movable cavity, and is clamped with the barrel.
Further, the sealing plug comprises a plug body and a sealing film, the middle of one end of the piston rod is hollow, the plug body is in threaded connection or interference fit with the cylinder, and the sealing film is arranged in the middle of the plug body.
Further, the liquid storage pipe comprises a pipe body and a sealing piece, one end of the pipe body is connected with the liquid inlet, and the other end of the pipe body is connected with the sealing piece.
Furthermore, the piston rod comprises a hand piece and a sealing piece, one end of the hand piece penetrates out of the movable cavity, the other end of the hand piece is connected with the sealing piece, and the sealing piece is used for sealing the liquid inlet and is arranged in the movable cavity in a sliding mode.
In order to solve the above technical problem, an embodiment of the present application further provides a microfluidic detection device, which adopts the following technical scheme:
a microfluidic detection device comprises a microfluidic chip and a quantitative sample adding device as described above; the micro-fluidic chip comprises a chip body, a functional cavity, a flow channel and a puncture device, wherein the functional cavity, the flow channel and the puncture device are arranged on the chip body, the flow channel is communicated with the functional cavity and the puncture device, and the puncture device is used for puncturing the sealing plug.
Further, the puncture device comprises a mounting piece and a puncture needle which are arranged on the chip body; the mounting pieces are arranged at intervals around the periphery of the puncture needle, the sealing plugs are matched with the mounting pieces, and the inlet of the flow passage is positioned at the bottom end of the puncture needle; the middle part of the pricking pin close to one end of the cylinder body is recessed to form a resisting groove.
Compared with the prior art, the embodiment of the application mainly has the following beneficial effects: in the whole sample adding process of the microfluidic chip, liquid is not required to be transferred from the outside for multiple times, the sample adding of the second liquid and the sample adding of the mixed liquid can be realized through the movement of the piston rod, the use of complicated and bulky instruments and equipment is avoided, the liquid transferring operation with the outside is reduced, the operation is simple, the detection efficiency is improved, the flexibility is improved, professional training and learning are not required, the operation can be carried out by hands, and the portable detection is realized; this application can the accurate control of application of sample volume through the control piston rod in the activity chamber towards the displacement who stores the chamber, has realized application of sample and the accurate of detecting and has controlled, can carry out the application of sample to the biological sample of multiple difference, has very extensive application scope, is fit for short-term test and family self-checking.
Drawings
In order to illustrate the solution of the present application more clearly, the drawings that are needed in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present application, and that other drawings can be obtained by those skilled in the art without inventive effort.
Fig. 1 is a schematic view of a quantitative sample adding device provided in an embodiment of the present application when the quantitative sample adding device and a microfluidic chip are mounted together;
FIG. 2 is a cross-sectional view of FIG. 1 with the third position-limiting member hidden;
FIG. 3 is a schematic diagram of the quantitative sample adding device of FIG. 2 separated from the microfluidic chip;
FIG. 4 is a perspective view of FIG. 1;
fig. 5 is an enlarged view at a in fig. 2.
Reference numerals: 1. a quantitative sample adding device; 10. a barrel; 11. a storage chamber; 12. a movable cavity; 13. a liquid inlet; 14. mounting grooves; 20. a liquid storage pipe; 21. a pipe body; 22. a closure member; 30. a sealing plug; 31. a plug body; 32. sealing films; 33. connecting grooves; 40. a piston rod; 41. a handpiece; 42. a seal member; 43. a limiting groove; 44. mounting a boss; 45. mounting holes; 50. a first limit piece; 60. a second limiting member; 70. a third limiting member;
2. a microfluidic chip; 80. a chip body; 90. a functional chamber; 100. a flow channel; 110. a puncturing device; 111. a mounting member; 112. a needle; 113. and a resisting groove.
Detailed Description
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "including" and "having," and any variations thereof, in the description and claims of this application and the description of the above figures are intended to cover non-exclusive inclusions. The terms "first," "second," and the like in the description and claims of this application or in the foregoing drawings are used for distinguishing between different objects and not for describing a particular sequential order.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings.
The embodiment of the present application provides a quantitative sample adding device 1, as shown in fig. 1 to 4, the quantitative sample adding device 1 includes: the device comprises a cylinder body 10, a liquid storage pipe 20, a sealing plug 30 and a piston rod 40; the cylinder 10 comprises a storage cavity 11 and a movable cavity 12 which are communicated with each other, a liquid inlet 13 communicated with the movable cavity 12 is formed in the cylinder wall of the cylinder 10, and the liquid storage pipe 20 is arranged at the liquid inlet 13; one end of the piston rod 40 penetrates through the movable cavity 12, and the other end of the piston rod 40 is used for sealing the liquid inlet 13 and is arranged in the movable cavity 12 in a sliding manner; the sealing plug 30 is movably connected with one end of the cylinder 10 far away from the piston rod 40 and is used for sealing the storage cavity 11; the storage cavity 11 is used for pre-loading a first liquid and storing a sample, the liquid storage tube 20 is used for pre-loading a second liquid, when the piston rod 40 moves towards a direction far away from the storage cavity 11, the sealing of the liquid inlet 13 is cancelled, and the quantitative sample adding device 1 is shaken to enable the second liquid to flow into the movable cavity 12, so that the second liquid and the first liquid are fully mixed.
The working principle of the quantitative sample adding device 1 provided by the embodiment of the application is as follows: first, a fixed amount of first liquid is filled in the storage chamber 11, and a fixed amount of second liquid is filled in the liquid storage tube 20; when sampling, the storage cavity 11 is placed upwards, the sealing plug 30 is taken down from the cylinder body 10, and the sealing of the storage cavity 11 is cancelled; after sampling, storing the sample in the storage cavity 11 pre-filled with the first liquid; subsequently, the sealing plug 30 is mounted back on the cylinder 10 to seal the storage chamber 11, and the first liquid and the sample are biologically reacted; then, the piston rod 40 is moved in a direction away from the storage cavity 11, so that the piston rod 40 cancels the sealing of the liquid inlet 13, the quantitative sample adding device 1 is shaken, the second liquid in the liquid storage tube 20 flows out of the liquid inlet 13 and enters the movable cavity 12, if the storage cavity 11 is full of the first liquid, the second liquid is mixed with the first liquid in the storage cavity 11 in the movable cavity 12, if the storage cavity 11 is not full of the first liquid, the second liquid flows into the storage cavity 11 and is mixed with the first liquid in the storage cavity 11, and the second liquid and the first liquid after the biological reaction of the sample are subjected to biological reaction again; then, after the quantitative sample adding device 1 is connected with the microfluidic chip, the microfluidic chip penetrates through the sealing plug 30 to be communicated with the storage cavity 11; and finally, moving the piston rod 40 towards the direction of the storage cavity 11, wherein the piston rod 40 pushes the mixed liquid in the cylinder 10 to enter the microfluidic chip, so that pressure sample injection of the microfluidic chip is realized.
The beneficial effects of a ration application of sample device 1 that this application embodiment provided are: be used for first liquid and deposit the sample in the storage cavity 11, directly place the sample in storage cavity 11 after the sampling and can carry out biological reaction, reduced the step that needs operating personnel to externally extract quantitative first liquid and sample and carry out biological reaction among the testing process. Integrate liquid storage tube 20 in the inlet 13 department of barrel 10 lateral wall, seal inlet 13 through piston rod 40 under the initial condition, prevent that the second liquid of pre-installation in the liquid storage tube 20 from getting into and storing chamber 11 in, when piston rod 40 removes to the direction of keeping away from and storing chamber 11, when cancelling the sealed of inlet 13, rock quantitative application of sample device 1, make the first liquid after second liquid and sample carry out the biological reaction mix, reduce the step that needs operating personnel externally to extract quantitative second liquid in the testing process once more. The piston rod 40 applies pressure to the mixed liquid in the cylinder 10, and the moving distance of the piston rod 40 towards the storage cavity 11 in the movable cavity 12 is controlled to realize the volume of the quantitative mixed liquid, and finally the quantitative pressure sample injection of the microfluidic chip is realized.
In the whole sample adding process of the microfluidic chip, liquid is not required to be transferred from the outside for multiple times, the sample adding of the second liquid and the sample adding of the mixed liquid can be realized through the movement of the piston rod 40, the use of complicated and bulky instruments and equipment is avoided, the liquid transferring operation with the outside is reduced, the operation is simple, the detection efficiency is improved, the flexibility is improved, professional training and learning are not required, the manual operation is not required, and the portable detection is realized; this application can the accurate control of application of sample volume through control piston rod 40 towards the displacement who stores chamber 11 in activity chamber 12, has realized the application of sample and the accurate of detecting and has controlled, can carry out the application of sample to the biological sample of multiple difference, has very extensive application scope, is fit for short-term test and family self-checking.
Further, the first liquid pre-filled in the storage cavity 11 of the cartridge 10 is a biological reaction reagent, such as a nucleic acid lysis solution, and the viral nucleic acid in the sample is released and purified by the first liquid. The second liquid pre-filled in the liquid storage tube 20 is a biological reaction reagent, such as a diluent, and the first liquid and the liquid after the viral nucleic acid reaction are diluted by the second liquid.
Further, the amount of the first liquid pre-filled in the storage chamber 11 may be between 50ul and 5ml, the size of the first liquid storage amount may be adjusted according to different biological reactions, and the amount of the second liquid pre-filled in the liquid storage tube 20 may also be determined according to biological reactions, which is not limited herein.
As shown in fig. 5, further, the quantitative sample adding device 1 further includes a first limiting member 50, the first limiting member 50 protrudes from the inner wall of the movable cavity 12, the liquid inlet 13 is located between the first limiting member 50 and the storage cavity 11, a limiting groove 43 is disposed on the outer wall of the piston rod 40, and the first limiting member 50 abuts against the limiting groove 43.
The position of the liquid inlet 13 is limited by the present application to be located between the first limiting member 50 and the storage cavity 11, so as to ensure that the piston rod 40 can move in the direction away from the storage cavity 11, and the piston rod 40 can be cancelled from being partially or completely sealed with the liquid inlet 13. The piston rod 40 is moved in a direction away from the storage cavity 11, and when the first position-limiting member 50 abuts against one end of the position-limiting groove 43 close to the storage cavity 11, the piston rod 40 cannot move in a direction away from the storage cavity 11; the position of the first limiting member 50 limits the moving range of the piston rod 40, the volume of the movable cavity 12, which is movable towards the direction far away from the storage cavity 11, of the piston rod 40 is used for quantifying the volume of the mixed liquid injected into the microfluidic chip, and the bigger the movable range of the piston rod 40 towards the direction far away from the storage cavity 11 is, the bigger the quantified volume of the mixed liquid is, namely the bigger the distance between one end, which is close to the sealing plug 30, of the piston rod 40 and the storage cavity 11 is, the bigger the quantified volume of the mixed liquid is, and the position of the first limiting member 50 or the length of the limiting groove 43 can be adjusted according to the quantified size of the mixed liquid; the liquid control device can accurately control 10ul to 5000ul of liquid.
As shown in fig. 1 to 4, further, the cylinder wall of the cylinder 10 is provided with at least two mounting grooves 14 communicating with the movable cavity 12, the liquid inlet 13 is located between any one of the mounting grooves 14 and the storage cavity 11, any one of the mounting grooves 14 is directly opposite to the limiting groove 43, and the first limiting member 50 penetrates through one of the mounting grooves 14 and then protrudes out of the inner wall of the cylinder 10.
The position of this application restriction inlet 13 is in between mounting groove 14 and the storage chamber 11, all can ensure that piston rod 40 can move to the direction of keeping away from storage chamber 11 when making first locating part 50 install on any mounting groove 14, makes piston rod 40 can cancel the part or all sealed with inlet 13. The movable range of the piston rod 40 can be controlled by adjusting the first limiting piece 50 to be arranged on one of the mounting grooves 14 close to the storage cavity 11 or one of the mounting grooves 14 far away from the storage cavity 11, so that the quantitative adjustment of the mixed liquid fed to the microfluidic chip can be realized; the installation position of the first limiting member 50 can be determined in the manufacturing process, or can be adaptively adjusted according to the biological reaction in the actual detection process, so that the application can sample a plurality of different biological samples, and has a very wide application range.
Further, the first limiting member 50 is a buckle piece, and the first limiting member 50 is connected to the mounting groove 14 in a buckling manner.
As shown in fig. 2, fig. 3 and fig. 5, further, the quantitative sample adding device 1 further includes a second limiting member 60, the second limiting member 60 protrudes from the inner wall of the cylinder 10, the second limiting member 60 is located between the storage cavity 11 and the movable cavity 12, and one end of the piston rod 40 adjacent to the storage cavity 11 abuts against the second limiting member 60. The second stopper 60 is used to prevent the piston rod 40 from moving towards the storage cavity 11 during transportation, so as to avoid the problem that the piston rod 40 cancels the sealing of the liquid inlet 13, which results in the second liquid mixing with the first liquid in advance; meanwhile, the second limiting member 60 is matched with the first limiting member 50, so that the moving distance of the piston rod 40 in the movable cavity 12 towards the storage cavity 11 is limited, and quantitative sample adding of the mixed liquid for sample feeding to the microfluidic chip is realized.
Further, the second limiting member 60 is an annular blocking piece, the second limiting member 60 is integrally formed with the barrel 10, and the storage cavity 11 and the movable cavity 12 are distinguished by the second limiting member 60.
As shown in fig. 1 and fig. 4, further, the quantitative sample adding device 1 further includes a third limiting member 70, and the third limiting member 70 is sleeved at one end of the piston rod 40 penetrating through the movable cavity 12 and is clamped with the cylinder 10. Before moving the piston rod 40 away from the storage chamber 11, the third stopper 70 is removed; the third limiting member 70 is used to prevent the piston rod 40 from moving in the direction away from the storage cavity 11 during transportation, so as to avoid the problem that the piston rod 40 cancels the sealing of the liquid inlet 13, which results in the second liquid mixing with the first liquid in advance.
Further, the quantitative sample adding device 1 comprises a second stopper 60 and a third stopper 70, which further limit the piston rod 40 and prevent the piston rod 40 from shifting during transportation.
As shown in fig. 3, further, the sealing plug 30 includes a plug body 31 and a sealing film 32, a middle portion of one end of the plug body 31, which is far away from the piston rod 40, is hollow, the plug body 31 is in threaded connection or interference fit with the cylinder 10, and the sealing film 32 is disposed in the hollow portion of the middle portion of the plug body 31. When the quantitative sample adding device 1 is connected with the microfluidic chip, the sealing plug 30 does not need to be taken down from the cylinder 10, and the microfluidic chip penetrates through the sealing film 32 to be communicated with the storage cavity 11, so that sample introduction of the microfluidic chip can be realized, and the operation steps are reduced.
Further, the plug body 31 is an annular plug, an annular connecting groove 33 is formed in the plug body 31, and the connecting groove 33 is in threaded connection with the end of the cylinder 10; the sealing performance of one end of the storage cavity 11 of the cylinder body 10 is improved.
Further, the sealing film 32 is made of a composite aluminum film, rubber or silica gel.
As shown in fig. 1 to 4, the liquid storage tube 20 further includes a tube body 21 and a sealing member 22, one end of the tube body 21 is connected to the liquid inlet 13, and the other end of the tube body 21 is connected to the sealing member 22. So that the production assembly of this application, install first locating part 50 in mounting groove 14, when the piston rod 40 is close to the one end and the butt of second locating part 60 of storing chamber 11, and sealed inlet 13 back, with third locating part 70 cover the piston rod 40 wear out movable cavity 12 one end and with barrel 10 joint, in following, with quantitative second liquid preloading body 21, again seal body 21 through sealing member 22 to prevent the second liquid leakage in the body 21, again in following with quantitative first liquid preloading body 11 of barrel 10, it is sealed with storing chamber 11 through sealing plug 30 at last.
Further, the sealing member 22 is a silicone cap or an aluminum film. When the sealing member 22 is a silica gel cap, the silica gel cap is in interference fit with the tube body 21, so that the tube body 21 is sealed; when the sealing member 22 is an aluminum film, the aluminum film is hot-pressed on the tube body 21, thereby sealing the tube body 21.
As shown in fig. 2 to fig. 5, further, the piston rod 40 includes a hand piece 41 and a sealing member 42, one end of the hand piece 41 penetrates through the movable cavity 12, the other end of the hand piece 41 is connected to the sealing member 42, and the sealing member 42 is used for sealing the liquid inlet 13 and is slidably disposed in the movable cavity 12.
Further, the limiting groove 43 is disposed on the outer wall of the handheld device 41, one end of the sealing element 42 adjacent to the storage cavity 11 abuts against the second limiting member 60, and the third limiting member 70 is sleeved on one end of the handheld device 41 penetrating out of the movable cavity 12.
Further, the sealing member 42 is in interference fit with the inner wall of the cartridge 10, and prevents the leakage of the pre-filled first liquid in the storage chamber 11.
Furthermore, one end of the hand-held piece 41 close to the sealing plug 30 is provided with a plurality of mounting bosses 44, and the sealing piece 42 is provided with mounting holes 45 matched with the mounting bosses 44. The seal 42 is interference fitted with the hand piece 41 by the mounting boss 44 and the mounting hole 45, and the seal 42 is prevented from falling off from the hand piece 41.
The embodiment of the present application further provides a microfluidic detection apparatus, as shown in fig. 1 to 4, the microfluidic detection apparatus includes a microfluidic chip 2 and the quantitative sample adding device 1 as described above; the micro-fluidic chip 2 comprises a chip body 80, and a functional chamber 90, a flow channel 100 and a puncture device 110 which are arranged on the chip body 80, wherein the flow channel 100 is communicated with the functional chamber 90 and the puncture device 110, and the puncture device 110 is used for puncturing the sealing film 32 on the sealing plug 30.
The working principle of the micro-fluidic detection equipment provided by the embodiment of the application is as follows: after the sealing plug 30 abuts against the puncture device 110, the puncture device 110 punctures the sealing film 32 on the sealing plug 30 to be communicated with the storage cavity 11, and the mixed liquid of the cylinder 10 enters the flow passage 100; when the piston rod 40 moves towards the piercing device 110, the mixed liquid is pushed to enter the functional chamber 90 from the flow channel 100, so that the pressure sample introduction of the microfluidic chip 2 is realized, and the detection of the functional chamber 90 is performed.
The beneficial effect of the micro-fluidic check out test set that this application embodiment provided does: the microfluidic detection equipment provided by the application is simple to operate, after the sealing plug 30 is abutted to the puncture device 110, the puncture device 110 punctures the sealing film 32 on the sealing plug 30 and is communicated with the storage cavity 11, the microfluidic chip 2 and the quantitative sample adding device 1 are connected together without complex instrument equipment, the operation is easy, and the microfluidic detection equipment is suitable for rapid detection and household self-detection; the driving force is applied to the mixed liquid through the piston rod 40, so that the mixed liquid enters the functional cavity 90, the simple quantitative sample adding device 1 is adopted, the sample adding of the microfluidic chip 2 is realized, the flexibility is improved, and professional training and learning and operation by hands are not needed.
In the whole sample adding process of the microfluidic chip 2, liquid is not required to be transferred from the outside for multiple times, and the sample adding of the second liquid and the sample adding of the mixed liquid can be realized through the movement of the piston rod 40, so that the use of complicated and bulky instruments and equipment is avoided, the operation is simple, the detection efficiency is improved, the flexibility is improved, professional training and learning are not required, the operation by hands is not required, and the portable detection is realized; this application can the accurate control of application of sample volume through control piston rod 40 towards the displacement who stores chamber 11 in activity chamber 12, has realized the accurate of application of sample and detection and has controlled, can carry out the application of sample to the biological sample of multiple difference, has very extensive application scope.
As shown in fig. 3, further, the puncturing device 110 includes a mounting member 111 and a puncturing needle 112 disposed on the chip body 80; the mounting parts 111 are arranged at intervals around the periphery of the puncture needle 112, the sealing plugs 30 are matched with the mounting parts 111, and the inlet of the flow passage 100 is positioned at the bottom end of the puncture needle 112; the middle of the needle 112 near one end of the cylinder 10 is recessed to form a stop groove 113.
The sealing plug 30 is installed in the installation part 111, so that the puncture needle 112 punctures the sealing film 32 on the sealing plug 30 to cancel the sealing of the storage cavity 11 by the sealing plug 30, the mixed liquid in the cylinder 10 flows out from the puncture position and enters the inlet of the flow passage 100, and the liquid cannot overflow the puncture device 110 under the action of the installation part 111; the stopping groove 113 of the puncture needle 112 increases the puncture point of the puncture needle 112, so that the puncture needle 112 can easily puncture the sealing film 32 on the sealing plug 30, and on the other hand, can prevent the broken sample (e.g., the probe head) from blocking the inlet of the flow channel 100.
Further, the flow channel 100 may be formed by machine tool machining, laser ablation, 3D printing, injection molding, or chemical etching.
Further, the width of the flow channel 100 is 0.01mm-10mm, and the depth is 0.01mm-10mm.
Further, the functional chamber 90 is pre-filled with a biological detection reagent for subsequent biological detection.
Further, the number of the function chambers 90 is at least one.
It is to be understood that the above-described embodiments are merely illustrative of some, but not restrictive, of the broad invention, and that the appended drawings illustrate preferred embodiments of the invention and do not limit the scope of the invention. This application is capable of embodiments in many different forms and is provided for the purpose of enabling a thorough understanding of the disclosure of the application. Although the present application has been described in detail with reference to the foregoing embodiments, it will be apparent to one skilled in the art that the present application may be practiced without modification or with equivalents of some of the features described in the foregoing embodiments. All equivalent structures made by using the contents of the specification and the drawings of the present application are directly or indirectly applied to other related technical fields and are within the protection scope of the present application.
Claims (10)
1. A quantitative sample application device, comprising: the device comprises a cylinder, a liquid storage pipe, a sealing plug and a piston rod; the cylinder body comprises a storage cavity and a movable cavity which are mutually communicated, a liquid inlet communicated with the movable cavity is formed in the cylinder wall of the cylinder body, and the liquid storage pipe is arranged at the liquid inlet; one end of the piston rod penetrates through the movable cavity, and the other end of the piston rod is used for sealing the liquid inlet and is arranged in the movable cavity in a sliding manner; the sealing plug is movably connected with one end of the cylinder body, which is far away from the piston rod, and is used for sealing the storage cavity;
the storage cavity is used for pre-filling a first liquid and storing a sample, the liquid storage tube is used for pre-filling a second liquid, and when the piston rod moves towards the direction far away from the storage cavity, the sealing of the liquid inlet is cancelled, so that the second liquid flows into the movable cavity, and the second liquid is mixed with the first liquid.
2. The quantitative sample adding device according to claim 1, further comprising a first limiting member protruding from the inner wall of the movable cavity, wherein the liquid inlet is located between the first limiting member and the storage cavity, the outer wall of the piston rod is provided with a limiting groove, and the first limiting member abuts against the limiting groove.
3. The quantitative sample adding device according to claim 2, wherein the barrel wall of the barrel body is provided with at least two mounting grooves communicated with the movable cavity, the liquid inlet is positioned between any one of the mounting grooves and the storage cavity, any one of the mounting grooves is opposite to the limiting groove, and the first limiting member penetrates through one of the mounting grooves and then protrudes out of the inner wall of the barrel body.
4. The device for loading quantitative samples according to any one of claims 1 to 3, wherein the device for loading quantitative samples further comprises a second limiting member, the second limiting member protrudes from the inner wall of the cylinder, the second limiting member is located between the storage chamber and the movable chamber, and one end of the piston rod, which is close to the storage chamber, abuts against the second limiting member.
5. The quantitative sample adding device according to any one of claims 1 to 3, further comprising a third limiting member, wherein the third limiting member is sleeved at one end of the piston rod penetrating out of the movable cavity and is clamped with the barrel.
6. The quantitative sample adding device according to any one of claims 1 to 3, wherein the sealing plug comprises a plug body and a sealing film, the middle part of one end of the plug body, which is far away from the piston rod, is hollow, the plug body is in threaded connection or interference fit with the cylinder body, and the sealing film is arranged in the middle of the plug body.
7. The quantitative sample adding device according to any one of claims 1 to 3, wherein the liquid storage tube comprises a tube body and a sealing member, one end of the tube body is connected with the liquid inlet, and the other end of the tube body is connected with the sealing member.
8. The quantitative sample adding device according to any one of claims 1 to 3, wherein the piston rod comprises a hand piece and a sealing member, one end of the hand piece penetrates through the movable cavity, the other end of the hand piece is connected with the sealing member, and the sealing member is used for sealing the liquid inlet and is slidably arranged in the movable cavity.
9. A microfluidic detection device, comprising a microfluidic chip and a quantitative sample adding device according to any one of claims 1 to 8; the micro-fluidic chip comprises a chip body, and a functional chamber, a flow channel and a puncture device which are arranged on the chip body, wherein the flow channel is communicated with the functional chamber and the puncture device, and the puncture device is used for puncturing the sealing plug.
10. The microfluidic detection device of claim 9, wherein the puncturing means comprises a mounting member and a puncturing needle provided on the chip body; the mounting pieces are arranged at intervals around the periphery of the puncture needle, the sealing plugs are matched with the mounting pieces, and the inlet of the flow passage is positioned at the bottom end of the puncture needle; the middle part of the pricking pin close to one end of the cylinder body is recessed to form a resisting groove.
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| CN202211081384.5A CN115155687B (en) | 2022-09-06 | 2022-09-06 | Quantitative sample adding device and microfluidic detection equipment |
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