CN116555007A - Detection device and detection method - Google Patents
Detection device and detection method Download PDFInfo
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- CN116555007A CN116555007A CN202210113338.2A CN202210113338A CN116555007A CN 116555007 A CN116555007 A CN 116555007A CN 202210113338 A CN202210113338 A CN 202210113338A CN 116555007 A CN116555007 A CN 116555007A
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- 238000001514 detection method Methods 0.000 title claims abstract description 54
- 239000012895 dilution Substances 0.000 claims abstract description 183
- 238000010790 dilution Methods 0.000 claims abstract description 183
- 238000005336 cracking Methods 0.000 claims abstract description 78
- 238000006243 chemical reaction Methods 0.000 claims abstract description 64
- 230000009471 action Effects 0.000 claims abstract description 10
- 230000009089 cytolysis Effects 0.000 claims description 51
- 239000000523 sample Substances 0.000 claims description 35
- 239000007788 liquid Substances 0.000 claims description 28
- 238000007789 sealing Methods 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 18
- 239000012470 diluted sample Substances 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 230000002934 lysing effect Effects 0.000 claims description 8
- 238000012360 testing method Methods 0.000 claims description 8
- 238000007865 diluting Methods 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims description 6
- 238000003776 cleavage reaction Methods 0.000 claims description 4
- 230000007017 scission Effects 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 12
- 230000004308 accommodation Effects 0.000 description 10
- 238000000197 pyrolysis Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000004891 communication Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000003321 amplification Effects 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
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- General Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention relates to a detection device and a detection method, wherein the detection device comprises: a dilution tube having a dilution chamber with an opening at one end; the cracking tube is accommodated in the dilution cavity and is provided with a cracking cavity with an opening at one end, and the closed end of the cracking cavity faces the closed end of the dilution cavity; the reaction tube is connected to the closed end of the dilution tube in a matching way and is positioned outside the dilution cavity, and the reaction tube is provided with a reaction cavity with an opening at one end; the puncture assembly is matched and connected with one end of the dilution pipe far away from the reaction pipe, and one end of the puncture assembly extends into the cracking cavity; the puncturing assembly can move along an axis under the action of external force to sequentially puncture the closed end of the cracking tube and the closed end of the dilution tube, so that the cracking cavity and the dilution cavity, and the dilution cavity and the reaction cavity are sequentially communicated. According to the detection device, the cracking tube, the dilution tube and the reaction tube are integrated, so that the experiment difficulty is effectively reduced, the contact opportunity between a sample and the external environment is remarkably reduced, the pollution probability is effectively reduced, and the detection accuracy is improved.
Description
Technical Field
The invention relates to the technical field of molecular detection, in particular to a detection device and a detection method.
Background
With the development of molecular detection techniques, nucleic acid detection techniques have been widely used. In the process of nucleic acid detection, a lysis tube for holding a lysis solution and providing a mixing space for the lysis solution and a sample solution carrying a sample, a dilution tube for diluting the sample lysis mixture, and a reaction tube for holding a reaction solution are required.
The specific process of nucleic acid detection is as follows: opening a tube cover of a cracking tube, adding sample liquid into the cracking tube containing the cracking liquid, mixing the sample liquid with the cracking liquid to form sample cracking mixed liquid, cracking the sample in the cracking tube, opening the tube cover of a dilution tube, quantitatively extruding the sample cracking mixed liquid from the cracking tube to the dilution tube, mixing the sample cracking mixed liquid with the dilution liquid in the dilution tube, opening the tube cover of a reaction tube, quantitatively extruding the diluted sample cracking mixed liquid from the dilution tube to the reaction tube, mixing the diluted sample cracking mixed liquid with the reaction liquid in the reaction tube, and performing sample amplification and fluorescence detection.
Because schizolysis pipe, dilution pipe and reaction tube separate independent setting, need frequent operation schizolysis pipe, dilution pipe and reaction tube at nucleic acid detection's in-process, and need frequent opening tube cap and add the operation, so, lead to the experimental step numerous, the operation degree of difficulty is great, and uses very inconvenient. In addition, frequent operation schizolysis pipe and reaction tube to carry out frequent joining operation, still increase the contact chance of sample liquid and external environment easily, lead to the sample contaminated, detect the precision and reduce. Moreover, the space occupied by the split cracking tube and the reaction tube is large, and the split cracking tube and the reaction tube are inconvenient to carry, so that the household nucleic acid detection cannot be realized.
Disclosure of Invention
Based on the above, it is necessary to provide a detection device and a detection method for solving the problems of high operation difficulty, easy pollution and inconvenient carrying of nucleic acid detection, which can achieve the technical effects of low operation difficulty, difficult pollution and convenient carrying.
According to one aspect of the present application, there is provided a detection apparatus comprising:
a dilution tube having a dilution chamber with an opening at one end;
the cracking tube is accommodated in the dilution cavity and is provided with a cracking cavity with an opening at one end, and the closed end of the cracking cavity faces to the closed end of the dilution cavity;
the reaction tube is connected to the closed end of the dilution tube in a matching way and is positioned outside the dilution cavity, and the reaction tube is provided with a reaction cavity with an opening at one end; and
the puncture assembly is matched and connected with one end of the dilution pipe far away from the reaction pipe, and one end of the puncture assembly extends into the cracking cavity;
the puncture assembly can move along an axis under the action of external force so as to puncture the closed end of the cracking tube and the closed end of the dilution tube in sequence, so that the cracking cavity is communicated with the dilution cavity, and the dilution cavity is communicated with the reaction cavity in sequence.
In one embodiment, a first piston part is arranged at one end part of the puncturing assembly, which extends into the cracking cavity, and is used for injecting the liquid in the cracking cavity into the dilution cavity when the puncturing assembly punctures the cracking tube and moves along the axis under the action of external force.
In one embodiment, the puncture assembly further comprises a puncture part protruding from one end of the first piston part facing the reaction tube, and the puncture part is used for puncturing the cracking tube and the dilution tube.
In one embodiment, a second piston part is arranged at one end part of the cracking tube, which faces to the reaction tube, and when the puncture assembly punctures the dilution tube and moves along the axis under the action of external force, the puncture assembly pushes the second piston part to inject the liquid in the dilution cavity into the reaction cavity.
In one embodiment, the lancing assembly is threadably coupled to the dilution tube, and the lancing assembly is capable of moving linearly along the axis while rotating about the axis relative to the dilution tube to sequentially lance the lysis tube and the dilution tube.
In one embodiment, the puncture assembly is provided with a first protruding portion, the cracking tube is provided with a second protruding portion, and in the process that the puncture assembly rotates around the axis relative to the dilution tube, the first protruding portion abuts against the second protruding portion to drive the cracking tube to synchronously rotate around the axis.
In one embodiment, at least one stirring blade is arranged on the outer wall of the cracking tube in a protruding mode, and the stirring blade is used for stirring the liquid in the dilution cavity.
In one embodiment, the lancing assembly includes a cap detachably coupled to an end of the dilution tube remote from the reaction tube, and a lance having an end coupled to the cap and an end extending into the cleavage cavity.
In one embodiment, the dilution tube comprises a dilution tube body and a first seal closing one end of the dilution tube body to form a closed end of the dilution chamber, the first seal being penetrable by the lancing assembly under external force;
the cracking tube comprises a cracking tube main body and a second sealing element, wherein the second sealing element seals one end of the cracking tube main body to form a closed end of the cracking cavity, and the second sealing element can be pierced by the piercing assembly under the action of external force.
According to an aspect of the present application, there is provided a detection method using the above detection device, including the steps of:
presetting related reagents in a cracking cavity and a diluting cavity respectively;
adding a sample into the cracking cavity and mixing the sample with a reagent in the cracking cavity to form a sample cracking mixed solution;
after a first preset time, rotating the puncture assembly to drive the puncture assembly to move along an axis until the cracking tube is punctured, so that the sample cracking mixed solution flows into the dilution cavity to be mixed with the reagent in the dilution cavity to form diluted sample mixed solution;
and after a second preset time, continuing to rotate the puncture assembly to drive the puncture assembly to move along the axis until the dilution tube is punctured, so that the diluted sample mixture flows into the reaction cavity.
Above-mentioned detection device has integrated schizolysis pipe, dilution pipe and reaction tube, when using above-mentioned detection device, only need to utilize the subassembly that pierces to pierce schizolysis pipe and dilution pipe in proper order can accomplish whole experimental step, need not frequent operation schizolysis pipe, dilution pipe and reaction tube in this process, consequently effectively reduced the experiment difficulty, show simultaneously and reduce the chance that sample contacted with external environment to effectively reduced pollution probability, improved the detection accuracy, and can be applied to different scenes such as family, be favorable to promoting the convenience of detection at home.
Drawings
FIG. 1 is a schematic diagram of a detecting device according to an embodiment of the present invention;
FIG. 2 is a schematic diagram showing the internal structure of the detecting device shown in FIG. 1;
FIG. 3 is an exploded view of the detection device of FIG. 1;
FIG. 4 is a schematic view of the detection device of FIG. 1 prior to piercing a first seal;
FIG. 5 is a schematic view of the detection device of FIG. 1 after the first seal is pierced;
FIG. 6 is a schematic view of the detection device of FIG. 1 after the second seal is pierced.
Reference numerals illustrate:
100. a detection device; 10. a base; 12. a receiving chamber; 121. a limit groove; 30. a dilution tube; 32. a dilution tube main body; 321. a first dilution accommodation; 3212. a limit rib; 3214. sealing ribs; 3216. an external thread; 323. a second dilution accommodation; 34. a first seal; 36. a dilution chamber; 50. a reaction tube; 52. a reaction chamber; 70. a pyrolysis tube; 72. a pyrolysis tube main body; 721. a pyrolysis accommodation portion; 7212. sealing ribs; 723. a second piston portion; 725. stirring blades; 727. a second protruding portion; 74. a second seal; 76. a lysing chamber; 90. a lancing assembly; 92. a cover body; 94. a piercing rod; 941. a first piston portion; 943. a puncturing portion; 945. a first boss.
Detailed Description
In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the invention, whereby the invention is not limited to the specific embodiments disclosed below.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present invention, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.
Referring to fig. 1 to 3, fig. 1 is a schematic structural diagram of a detection device 100 according to an embodiment of the invention; FIG. 2 is a schematic diagram showing the internal structure of a detecting device 100 according to an embodiment of the present invention; fig. 3 shows an exploded view of the detection device 100 in an embodiment of the invention.
An embodiment of the present invention provides a testing device 100 comprising a base 10, a dilution tube 30 for containing a dilution liquid, a lysis tube 70 for containing a lysis liquid, a reaction tube 50 for containing a reaction liquid, and a lancing assembly 90. Wherein the dilution tunnel 30 has a dilution chamber 36, one end of the dilution chamber 36 is opened to form an oppositely disposed open end and a closed end of the dilution tunnel 30, and the closed end of the dilution tunnel 30 extends into the base 10. The tube 70 is received in the dilution chamber 36, the tube 70 having a chamber 76 open at one end, the closed end of the chamber 76 facing the closed end of the dilution chamber 36. The reaction tube 50 is accommodated in the base 10, one end of the reaction tube 50 is coupled to the closed end of the dilution tube 30 and is located outside the dilution chamber 36, and the reaction tube 50 has a reaction chamber 52 with an opening at one end. Lancing assembly 90 is coupled to the end of dilution tube 30 remote from reaction tube 50, with one end of lancing assembly 90 extending into lysing chamber 76. Wherein the lancing assembly 90 is movable along the axis L under an external force to sequentially lance the closed end of the lysis tube 70 and the closed end of the dilution tube 30, thereby allowing the lysis chamber 76 to communicate with the dilution chamber 36, and the reaction chamber 52 in sequence.
When the detection device 100 is used, a sample can be first added into the lysis chamber 76 and mixed with the lysis solution to form a sample lysis mixture, after a period of time of lysis, the sealing end of the lysis tube 70 is pierced by the piercing assembly 90, the sample lysis mixture in the lysis chamber 76 enters the dilution chamber 36 to be mixed with the dilution solution in the dilution chamber 36 to form a diluted sample lysis mixture, and then the sealing end of the dilution tube 30 is pierced by the piercing assembly 90, and the diluted sample lysis mixture in the dilution chamber 36 enters the reaction chamber 52 to be mixed with the reaction solution for amplification and fluorescence detection.
Therefore, the above-mentioned detection device 100 integrates the cracking tube 70, the dilution tube 30 and the reaction tube 50, and when the above-mentioned detection device 100 is used, the whole experimental step can be completed by only using the puncture assembly 90 to puncture the cracking tube 70 and the dilution tube 30 in sequence, and frequent operation of the cracking tube 70, the dilution tube 30 and the reaction tube 50 is not needed in the process, so that the experimental difficulty is effectively reduced, and meanwhile, the contact probability of the sample and the external environment is obviously reduced, so that the pollution probability is effectively reduced, the detection accuracy is improved, and the detection device can be applied to different scenes such as families, and is favorable for improving the convenience of home detection.
With continued reference to fig. 2 and 3, the base 10 is a hollow housing structure, and has an accommodating cavity 12 with an opening at one end, a step surface wound in the circumferential direction is formed in the accommodating cavity 12 to limit the dilution pipe 30, at least one limiting groove 121 is formed in a cavity wall of the accommodating cavity 12, and the limiting groove 121 extends along an axial direction of the base 10 to prevent the dilution pipe 30 from rotating relative to the base 10. It will be appreciated that the specific configuration of the base 10 is not limited thereto and may be set as desired.
The dilution pipe 30 includes a dilution pipe body 32 and a first seal 34. The dilution pipe main body 32 has a tubular structure with both ends open, and includes a first dilution accommodating portion 321 and a second dilution accommodating portion 323. One end of the first dilution accommodating portion 321 forms an open end of the accommodating chamber 12, the second dilution accommodating portion 323 is connected to the other end of the first dilution accommodating portion 321, the outer diameter of the second dilution accommodating portion 323 is smaller than that of the first dilution accommodating portion 321, and the inner diameter of the second dilution accommodating portion 323 is also smaller than that of the first dilution accommodating portion 321. The second dilution receiving part 323 has a communication hole formed at an end far from the first dilution receiving part 321, and the first sealing member 34 is disposed at an end far from the first dilution receiving part 321 of the second dilution receiving part 323 to close the communication hole to form a closed end of the dilution chamber 36, so that the first sealing member 34 can be pierced by the piercing assembly 90 under the action of external force.
Further, at least one limiting rib 3212 is protruding from an outer sidewall of the first dilution receiving portion 321 near one end of the second dilution receiving portion 323, and the limiting rib 3212 is matched with the limiting groove 121 of the base 10 to prevent the dilution pipe 30 from rotating relative to the base 10. The outer side wall of the first dilution receiving portion 321 is further provided with a sealing rib 3214 and an external thread 3216, the sealing rib 3214 is located at an end portion of the first dilution receiving portion 321 far away from the second dilution receiving portion 323, the sealing rib 3214 is movably and sealingly connected with the puncture assembly 90, and the external thread 3216 is located between the sealing rib 3214 and the second dilution receiving portion 323 and is in threaded connection with the puncture assembly 90. In this way, the second dilution accommodating portion 323 extends into the accommodating cavity 12 of the base 10, one end of the first dilution accommodating portion 321 provided with the limiting rib 3212 extends into the accommodating cavity 12, the first dilution accommodating portion 321 is connected with the end face of one end of the second dilution accommodating portion 323 to abut against the step face in the accommodating cavity 12, and the limiting rib 3212 is limited in the limiting groove 121. When the first seal 34 is pierced by the lancing assembly 90, liquid in the dilution chamber 36 can flow out through the breach of the first seal 34.
The reaction tube 50 is accommodated in the second accommodation portion of the base 10, and one end of the reaction tube 50 is sleeved outside the second dilution accommodation portion 323 of the dilution tube 30. The open end of the reaction chamber 52 of the reaction tube 50 faces the first seal 34 of the dilution tube 30. When the first seal 34 of the dilution tube 30 is pierced by the lancing assembly 90, liquid in the dilution chamber 36 can flow into the reaction chamber 52 through the breach of the first seal 34.
The pyrolysis tube 70 includes a pyrolysis tube body 72 and a second seal 74. The main body 72 of the pyrolysis tube has a tubular structure with both ends open, and includes a pyrolysis housing portion 721 and a second piston portion 723. One end of the cracking accommodation portion 721 forms an open end of the cracking chamber 76, and an outer side wall of the end is provided with a sealing rib 7212 to be movably and hermetically connected with the open end of the dilution tube 30, a second piston portion 723 is connected to the other end of the cracking accommodation portion 721, an outer diameter of the second piston portion 723 is matched with an inner diameter of the second dilution accommodation portion 323 of the dilution tube 30, a communication hole is formed in one end of the second piston portion 723, which is close to the second dilution accommodation portion 323, a second sealing element 74 is arranged in one end of the second piston portion 723, which is close to the second dilution accommodation portion 323, to close the communication hole, so that a closed end of the cracking chamber 76 is formed, and the second sealing element 74 can be pierced by the piercing assembly 90 under the action of external force.
Thus, when the second seal 74 is pierced, the liquid in the cleavage cavity 76 can flow into the dilution cavity 36 through the break of the second seal 74, the second piston portion 723 of the cleavage tube 70 extends into the second dilution receiving portion 323 of the dilution tube 30, and the second piston portion 723 can quantitatively inject the liquid in the dilution cavity 36 into the reaction cavity 52.
With continued reference to FIGS. 2 and 3, lancing assembly 90 includes a cap 92 and a lance 94, wherein cap 92 is removably coupled to an end of dilution tube 30 remote from reaction tube 50, one end of lance 94 is coupled to cap 92, and the other end of lance 94 extends into lysis chamber 76. Specifically, the cover 92 has a cylindrical structure with an opening at one end, the cover 92 is covered on an outer side wall of one end of the dilution pipe 30 extending out of the accommodating cavity 12, and one end of the cover 92 near the opening end thereof is provided with an internal thread to be in threaded connection with the outer side wall of the dilution pipe 30.
In this manner, because the dilution tube 30 is secured in a circumferential direction relative to the base 10 by the stop ribs 3212 and the stop slots 121, the lancing assembly 90 is capable of moving linearly along the axis L to lance the lysis tube 70 and the dilution tube 30 in sequence while rotating about the axis L relative to the dilution tube 30.
One end of the piercing rod 94 is inserted into one end of the cover 92 far away from the base 10, one end of the piercing rod 94 extending into the cracking chamber 76 is provided with a first piston portion 941 and a piercing portion 943, the outer diameter of the first piston portion 941 is matched with the inner diameter of the second piston portion 723, the piercing portion 943 is convexly arranged at one end of the first piston portion 941 facing the reaction tube 50, and one end of the piercing portion 943 far away from the first piston portion 941 is in a needle-shaped structure for piercing the second sealing member 74 of the cracking tube 70 and the first sealing member 34 of the dilution tube 30.
In this manner, cap 92 may be rotated to move lancing assembly 90 downward in the direction of axis L, lancing portion 943 may continue to move along axis L after lancing second seal 74 of lance 70, and first piston portion 941 may inject fluid within lancing chamber 76 into dilution chamber 36. After the first piston portion 941 injects the liquid in the cracking chamber 76 into the diluting chamber 36, the cap 92 is further rotated to move the lancing assembly 90 downward along the axis L, the lancing portion 943 pierces the first sealing member 34 of the diluting tube 30 and then moves along the axis L, and the second piston portion 723 of the cracking tube 70 injects the liquid in the diluting chamber 36 into the reaction chamber 52.
In some embodiments, the outer sidewall of lance 94 is provided with a first projection 945 and the inner sidewall of the cracking tube 70 is provided with a second projection 727, and the outer wall of the end of the cracking tube 70 to which the second piston portion 723 of the cracking tube 70 is connected is provided with at least one stirring blade 725. During rotation of the lancing assembly 90 relative to the separation tube 70 about the axis L, the first projection 945 abuts against the second projection 727 to drive the separation tube 70 to rotate synchronously about the axis L, so that the liquid in the dilution chamber 36 is stirred by the stirring blade 725 to mix the liquid in the dilution chamber 36 sufficiently.
As a preferred embodiment, the pyrolysis tube 70 is provided with four stirring blades 725, and the four stirring blades 725 are circumferentially spaced apart. It will be appreciated that the shape and number of the stirring vanes 725 are not limited and may be set as desired to meet various requirements.
The detection device 100 operates as follows:
when the test device 100 is not in use, the dilution chamber 36 contains a diluent and the lysis chamber 76 contains a lysis solution. When using the test device 100, an operator may unscrew the lancing assembly 90 exposing the open end of the lysis chamber 76 so that a sample may be added to the lysis solution from the open end of the lysis chamber 76.
As shown in FIG. 4, after adding the sample to the lysis solution, the operator may reinstall the lancing assembly 90 on the dilution tube 30, with the cap 92 covering the end of the dilution tube 30 remote from the first seal 34 and not threadably engaged with the dilution tube 30, and with the lancing portion 943 aligned with the first seal 34 and spaced from the first seal 34.
As shown in FIG. 5, rotating cap 92 threadably engages cap 92 of lancing assembly 90 with dilution tube 30, and during rotation of cap 92, lancing lever 94 moves downwardly simultaneously along axis L to puncture second seal 74, first piston portion 941 of lancing lever 94 extends into second piston portion 723 to quantitatively inject sample lysis mixture in lysis chamber 76 into dilution chamber 36 for dilution. At the same time, the first projection 945 of the lance 94 abuts the second projection 727 (shown in FIG. 2) of the cracking tube 70, and the cracking tube 70 is rotated about the axis L by the lancing assembly 90 to agitate the liquid in the dilution chamber 36.
After the sample lysis mixture is sufficiently filled, as shown in fig. 6, the cap 92 is continuously rotated to continuously move the puncture rod 94 downward along the axis L to puncture the first sealing member 34, and at the same time, the first piston portion 941 of the puncture rod 94 is moved downward against the second piston portion 723 of the lysis tube 70, and the second piston portion 723 is inserted into the second dilution receiving portion 323, so that the diluted sample lysis mixture in the second dilution receiving portion 323 is quantitatively injected into the reaction chamber 52.
The application also provides a detection method of the detection device 100, which comprises the following steps:
s110: the relevant reagents are respectively preset in the cracking cavity and the dilution cavity.
Specifically, the lysis solution is pre-placed in the lysis chamber 76 and the dilution solution is pre-placed in the dilution chamber 36.
S120: the sample is added to the lysis chamber 76 and mixed with the reagents in the lysis chamber 76 to form a sample lysis mixture.
Specifically, in the initial state, the first seal 34 seals one end of the lysing chamber 76 to seal the lysing solution in the lysing chamber 76. The operator may unscrew the lancing assembly 90, insert the pharyngeal swab into the lysate of the lysis chamber 76, discard the pharyngeal swab after 5-10 rotations and reinstall the lancing assembly 90 onto the dilution tube 30.
S130: after a first predetermined time, the lancing assembly 90 is rotated to drive the lancing assembly 90 along an axis until the lysis tube 70 is lanced, such that the sample lysis mixture flows into the dilution chamber 36 and mixes with the reagents in the dilution chamber 36 to form a diluted sample mixture.
Specifically, after a first predetermined time, the lancing assembly 90 is rotated, the lancing assembly 90 is moved downward along the axis L while rotating relative to the dilution tube 30, the lancing portion 943 pierces the second seal 74 closing the lysis chamber 76, the first piston portion 941 of the lancing lever 94 extends into the second piston portion 723, and the sample lysis mixture in the lysis chamber 76 is dosed into the dilution chamber 36 for dilution. At the same time, the lysis tube 70 is rotated about the axis L by the lancing assembly 90 to agitate the liquid in the dilution chamber 36. As a preferred embodiment, the first piston portion 941 injects 100 microliters of sample lysis mixture into the dilution chamber 36.
S140: after a second predetermined time, the lancing assembly 90 is rotated continuously to drive the lancing assembly 90 to move along the axis until the dilution tube 30 is lanced, so that the diluted sample mixture flows into the reaction chamber 52.
Specifically, after a second preset time, the lancing assembly 90 is continuously rotated, the lancing portion 943 is continuously moved in the direction of the axis L to puncture the first sealing member 34 closing the dilution chamber 36, the lysis tube 70 is moved downward along the axis L by the pushing of the lancing lever 94, and the second piston portion 723 is inserted into the second dilution receiving portion 323 to inject the diluted sample lysis mixture in the dilution chamber 36 into the reaction chamber 52. As a preferred embodiment, the second piston portion 723 injects 50 μl of the diluted sample lysis mixture into the reaction tube 50.
It can be appreciated that the first preset time and the second preset time can be flexibly set according to the reaction time to meet the experimental requirement.
According to the detection device 100 and the detection method of the detection device 100, after a sample is added, the experimental steps of splitting, diluting and leading the sample into the reaction tube 50 can be automatically realized only by rotating the cover body 92, detection is carried out according to the quantitative volume, and the process of reagent mixing is carried out in the detection device 100, so that the interference of the external environment is reduced, the detection precision is effectively improved, the operation is simple and convenient, the error rate is low, the assembly is convenient, and the detection efficiency and the user experience are effectively improved.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (10)
1. A detection apparatus, characterized by comprising:
a dilution tube having a dilution chamber with an opening at one end;
the cracking tube is accommodated in the dilution cavity and is provided with a cracking cavity with an opening at one end, and the closed end of the cracking cavity faces to the closed end of the dilution cavity;
the reaction tube is connected to the closed end of the dilution tube in a matching way and is positioned outside the dilution cavity, and the reaction tube is provided with a reaction cavity with an opening at one end; and
the puncture assembly is matched and connected with one end of the dilution pipe far away from the reaction pipe, and one end of the puncture assembly extends into the cracking cavity;
the puncture assembly can move along an axis under the action of external force so as to puncture the closed end of the cracking tube and the closed end of the dilution tube in sequence, so that the cracking cavity is communicated with the dilution cavity, and the dilution cavity is communicated with the reaction cavity in sequence.
2. The testing device of claim 1, wherein a first piston portion is provided at an end of the lancing assembly extending into the lysing chamber, the first piston portion being configured to inject fluid from the lysing chamber into the dilution chamber when the lancing assembly is lancing the lysing tube and is moved along the axis by an external force.
3. The testing device of claim 2, wherein the lancing assembly further comprises a lancing portion protruding from an end of the first piston portion facing the reaction tube, the lancing portion being configured to lance the lysis tube and the dilution tube.
4. The device according to claim 1, wherein the tip of the tube is provided with a second piston portion facing the end of the tube, and the lancing assembly pushes the second piston portion to inject the liquid in the dilution chamber into the reaction chamber when the lancing assembly pierces the dilution tube and moves along the axis under the action of an external force.
5. The test device of claim 1, wherein the lancing assembly is threadably coupled to the dilution tube, the lancing assembly being capable of moving linearly along the axis while rotating about the axis relative to the dilution tube to sequentially lance the lysis tube and the dilution tube.
6. The testing device of claim 5, wherein the lancing assembly includes a first protrusion and the lysis tube includes a second protrusion, and wherein the first protrusion abuts the second protrusion to drive the lysis tube to rotate synchronously about the axis during rotation of the lancing assembly relative to the dilution tube about the axis.
7. The device of claim 6, wherein at least one stirring blade is protruding from the outer wall of the tube, the stirring blade being configured to stir the liquid in the dilution chamber.
8. The device of claim 1, wherein the lancing assembly comprises a cap detachably coupled to an end of the dilution tube remote from the reaction tube and a lance having an end coupled to the cap and an end extending into the cleavage cavity.
9. The test device of claim 1, wherein the dilution tunnel comprises a dilution tunnel body and a first seal closing one end of the dilution tunnel body to form a closed end of the dilution chamber, the first seal being penetrable by the lancing assembly under an external force;
the cracking tube comprises a cracking tube main body and a second sealing element, wherein the second sealing element seals one end of the cracking tube main body to form a closed end of the cracking cavity, and the second sealing element can be pierced by the piercing assembly under the action of external force.
10. A detection method using the detection device according to any one of claims 1 to 9, comprising the steps of:
presetting related reagents in a cracking cavity and a diluting cavity respectively;
adding a sample into the cracking cavity and mixing the sample with a reagent in the cracking cavity to form a sample cracking mixed solution;
after a first preset time, rotating the puncture assembly to drive the puncture assembly to move along an axis until the cracking tube is punctured, so that the sample cracking mixed solution flows into the dilution cavity to be mixed with the reagent in the dilution cavity to form diluted sample mixed solution;
and after a second preset time, continuing to rotate the puncture assembly to drive the puncture assembly to move along the axis until the dilution tube is punctured, so that the diluted sample mixture flows into the reaction cavity.
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CN116718449A (en) * | 2023-08-10 | 2023-09-08 | 江苏美克医学技术有限公司 | Medical sample pretreatment mechanism, pretreatment device and use method |
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CN116718449A (en) * | 2023-08-10 | 2023-09-08 | 江苏美克医学技术有限公司 | Medical sample pretreatment mechanism, pretreatment device and use method |
CN116718449B (en) * | 2023-08-10 | 2023-11-21 | 江苏美克医学技术有限公司 | Medical sample pretreatment mechanism, pretreatment device and use method |
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