CN219279894U

CN219279894U - Nucleic acid amplification detection device

Info

Publication number: CN219279894U
Application number: CN202320177364.1U
Authority: CN
Inventors: 王彩英; 赵永飞; 王超; 李萍
Original assignee: Zhejiang Fengying Medical Plastic Co ltd
Current assignee: Zhejiang Fengying Medical Plastic Co ltd
Priority date: 2023-01-31
Filing date: 2023-01-31
Publication date: 2023-06-30
Anticipated expiration: 2033-01-31

Abstract

The utility model discloses a nucleic acid amplification detection device which comprises a shell and a reaction tube, wherein an upper cavity and a lower cavity are arranged in the shell, a connecting cylinder communicated with the upper cavity is arranged on the shell, the reaction tube is detachably connected in the connecting cylinder, an annular sealing film is arranged at the bottom of the reaction tube, a conical column is arranged at the bottom of the connecting cylinder, detection test paper is arranged in the upper cavity, a puncture assembly and an observation window are respectively arranged on the shells positioned at two sides of the connecting cylinder, a through hole is arranged on the bottom wall of the upper cavity below the puncture assembly, and a sealing film is connected in the through hole in a sealing manner. The nucleic acid amplification reaction and the detection reaction process are always in a sealed state, the amplification liquid cannot diffuse to the outside to generate aerosol, and residual nucleic acid is removed after the detection is finished, so that the exposure pollution risk existing before and after the nucleic acid amplification detection is reduced.

Description

Nucleic acid amplification detection device

Technical Field

The utility model relates to the technical field of nucleic acid detection devices, in particular to a nucleic acid amplification detection device.

Background

Nucleic acid amplification is a general term for a large class of technical methods, mainly including polymerase chain reaction, fluorescent quantitative PCR, isothermal amplification techniques, and the like. At present, the nucleic acid amplification technology is mainly applied to basic research and clinical aspects, can provide target genes through genetic engineering, is widely applied to individual identification, paternity test, immune matching, disease diagnosis and the like, and becomes an indispensable part of molecular biology research.

The detection of the nucleic acid amplification result in the prior art mainly comprises fluorescent dye, indicator, ultraviolet absorption detection, agarose gel electrophoresis and the like. The fluorescent dye and the indicator are generally added into a nucleic acid amplification reaction solution before the reaction starts or after the reaction ends, an isothermal amplification reaction tube is opened, and the fluorescent dye or the indicator is added into a reaction product, so that the result of the loop-mediated isothermal amplification reaction is detected. Because the fluorescent dye or the indicator can influence the nucleic acid amplification reaction to a certain extent, after the reaction is finished, the operation of adding the fluorescent dye or the indicator can lead reaction products to be in direct contact with air, aerosol pollution is easy to be caused,

disclosure of Invention

The present utility model has been made to solve the above-described problems, and provides a nucleic acid amplification detection apparatus.

The utility model adopts the following technical scheme:

the utility model provides a nucleic acid amplification detection device, includes casing and reaction tube, be equipped with cavity and lower cavity in the casing, be equipped with the connecting cylinder with last cavity intercommunication on the casing, the reaction tube can dismantle the connection in the connecting cylinder, and the reaction tube bottom is equipped with an annular sealing membrane, the connecting cylinder bottom is equipped with the awl post, be equipped with test paper in the last cavity, be equipped with puncture subassembly and observation window respectively on the casing that is located the connecting cylinder both sides, be equipped with the through-hole on the last cavity diapire of puncture subassembly below, sealing connection has the sealing membrane in the through-hole.

As a preferable technical scheme of the utility model, the bottom of the connecting cylinder is provided with a liquid inlet communicated with the upper cavity, an ejector rod is fixed in the middle of the liquid inlet through a bracket, the conical column is fixed on the bracket and positioned below the sealing film, and the reaction tube is in threaded connection in the connecting cylinder.

As a preferable technical scheme of the utility model, the upper end of the connecting cylinder is provided with an elastic sealing ring.

As a preferable technical scheme of the utility model, the puncture assembly comprises an elastic sealing sleeve, a conical strip and a spring, wherein a mounting groove is formed in a shell body positioned at one side of the connecting cylinder, a jack communicated with the upper cavity is formed in the bottom of the mounting groove, the lower end of the sealing sleeve is connected in the mounting groove in a sealing manner, the conical strip is movably inserted in the jack, a pressing part is arranged at the upper end of the conical strip, and the spring is sleeved outside the conical strip positioned between the pressing part and the bottom surface of the mounting groove.

As a preferable technical scheme of the utility model, a drainage rod is fixedly connected in the through hole below the sealing film.

The beneficial effects of the utility model are as follows:

the nucleic acid amplification reaction is carried out in a sealed reaction tube, when the detection reaction is carried out, the reaction tube is connected with a connecting cylinder in a sealing way, the bottom of the reaction tube is pierced to enable amplification liquid to enter an upper cavity to react with detection test paper, after the detection is finished, the residual nucleic acid amplification liquid is led into a lower cavity to react with the reaction liquid to remove the residual nucleic acid in the amplification liquid, the amplification liquid is always in a sealing state in the process and cannot be diffused to the outside to generate aerosol, and the residual nucleic acid is removed after the detection is finished, so that the exposure pollution risk existing before and after the nucleic acid amplification detection is reduced.

Drawings

FIG. 1 is a front view of the present utility model;

FIG. 2 is a schematic diagram of the internal structure of the present utility model;

FIG. 3 is a top view of the connecting cylinder;

FIG. 4 is a bottom view of the reaction tube.

The symbols in the drawings illustrate:

1: housing, 101: upper cavity, 102: lower cavity, 2: reaction tube, 3: connecting cylinder, 4: sealing film, 5: conical column, 6: test paper, 7: viewing window, 8: sealing film, 9: liquid inlet, 10: support, 11: ejector rod, 12: sealing ring, 13: sealing sleeve, 14: taper bar, 15: spring, 16: jack, 17: pressing portion, 18: a drainage bar.

Detailed Description

The utility model will now be described in further detail with reference to the accompanying drawings.

In the embodiments, it should be understood that the orientation or positional relationship indicated by the terms "middle," "upper," "lower," "top," "bottom," "right," "left," "above," "below," "back," "front," "middle," "outside," "inside," etc. are based on the orientation or positional relationship shown in the drawings, merely for convenience of description of the present embodiments, and are not intended to indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present embodiments.

As shown in fig. 1 to 4, a nucleic acid amplification detection device comprises a housing 1 and a reaction tube 2, an upper cavity 101 and a lower cavity 102 are arranged in the housing 1, the housing 1 is sealed, the interior of the housing is divided into the upper cavity 101 and the lower cavity 102 by a partition board, a connecting tube 3 communicated with the upper cavity 101 is arranged on the housing 1, the reaction tube 2 is detachably connected in the connecting tube 3, and the reaction tube 2 and the connecting tube 3 are in a sealed connection state when the two are connected, an annular sealing film 4 is arranged at the bottom of the reaction tube 2, so that when the sealing film 4 is broken, a circular notch is correspondingly formed at the bottom of the reaction tube 2, a conical column 5 is arranged at the bottom of the connecting tube 3, the conical column 5 is positioned under the sealing film 4, the sealing film 4 is scratched by the conical column 5, a detection test paper 6 is arranged in the upper cavity 101, the detection test paper 6 is used for reacting with nucleic acid amplification liquid in the reaction tube 2, whether target nucleic acid exists is judged by the change of the detection paper 6, a puncture window 7 is arranged on the housing 1 and the two sides of the connecting tube 3 respectively, and a puncture window 7 is arranged in the upper cavity 101 and a puncture hole is also arranged in the upper cavity 101, namely, the puncture test paper is communicated with a puncture window is arranged in the puncture window 101.

Further, the bottom of the connecting cylinder 3 is provided with a liquid inlet 9 communicated with the upper cavity 101, a push rod 11 is fixed in the middle of the liquid inlet 9 through a support 10, wherein the support 10 is composed of a plurality of spokes arranged in a radiation mode, the conical column 5 is fixed on one of the spokes of the support 10, the conical column 5 is located below the sealing film 4, namely, the distance between the conical column 5 and the center of the liquid inlet 9 is equal to the radius of the annular sealing film 4, the height of the push rod 11 is smaller than the height of the conical column 5, so that the bottom of the reaction tube 2 is contacted with the conical column 5 firstly, and the reaction tube 2 is connected in the connecting cylinder 3 in a threaded mode. When the reaction tube 2 is connected into the connecting cylinder 3 in a downward threaded manner until the bottom of the reaction tube 2 is contacted with the conical column 5, the conical column 5 is used for puncturing the sealing film 4, when the reaction tube 2 continuously rotates downwards, the conical column 5 rotates for a circle relative to the sealing film 4, so that the annular sealing film 4 is completely scratched, the reaction tube 2 continuously rotates downwards and is contacted with the ejector rod 11, then the bottom of the reaction tube 2 positioned at the inner side of the sealing film 4 is jacked up through the supporting effect of the ejector rod 11, so that a larger circular liquid outlet is formed at the bottom end of the reaction tube 2, nucleic acid amplification liquid in the reaction tube 2 conveniently enters the upper cavity 101 and reacts with the contact of the detection test paper 6, and the situation that the puncturing port is too small and the liquid in the reaction tube 2 cannot be pressed into the upper cavity 101 through the atmospheric pressure due to the reason of isolation from the outside is avoided.

Further, an elastic sealing ring 12 is arranged at the upper end of the connecting cylinder 3, and the sealing ring 12 ensures that the reaction tube 2 is in a sealed connection state when being connected with the connecting cylinder 3.

Further, the puncture assembly comprises an elastic sealing sleeve 13, a conical strip 14 and a spring 15, a mounting groove is formed in the shell 1 on one side of the connecting cylinder 3, a jack 16 communicated with the upper cavity 101 is formed in the bottom of the mounting groove, the lower end of the sealing sleeve 13 is connected in the mounting groove in a sealing mode, meanwhile, the jack 16 is covered, the whole shell 1 is in a sealing state, the conical strip 14 is movably inserted into the jack 16, a pressing portion 17 is arranged at the upper end of the conical strip 14, the lower end of the conical strip 14 extends to the upper side of the sealing film 8, and the spring 15 is sleeved outside the conical strip 14 between the pressing portion 17 and the bottom surface of the mounting groove. In use, the elastic sealing sleeve 13 is pressed, the conical strip 14 is pressed downwards and pierces the sealing film 8, and the upper cavity 101 and the lower cavity 102 are communicated.

Further, a drainage rod 18 is fixedly connected in the through hole below the sealing film 8, and the drainage rod 18 can be made of water absorbing materials such as cotton and sponge.

The application principle of the utility model is as follows:

at first, the upper cavity 101 is adhered with the test paper 6, the lower cavity 102 is stored with the reaction liquid capable of reacting with the nucleic acid sample, the reaction tube 2 is filled with the nucleic acid sample mixed with the amplification reagent, wherein the reaction tube 2 can be a test tube with a cover body, when the amplification reaction is carried out for a period of time, the reaction tube 2 is connected into the connecting cylinder 3 in a threaded manner, the sealing membrane 4 is broken through the conical column 5, a larger liquid outlet is formed at the bottom of the reaction tube 2, the liquid in the reaction tube 2 conveniently enters the upper cavity 101 to react with the test paper 6, after a period of time, the test paper 6 is checked whether to change or not through the observation window 7, after the record is finished, the sealing sleeve 13 is pressed to puncture the sealing membrane 8, the liquid in the upper cavity 101 and the lower cavity 102 overcomes the pressure difference through the water absorption capacity of the drainage rod 18 to carry out the reaction, the residual nucleic acid in the upper cavity 101 is completely removed through the reaction liquid, and the hidden danger of aerosol pollution caused by the residual nucleic acid in the subsequent treatment process is eliminated.

Finally, it should be noted that: these embodiments are merely for illustrating the present utility model and do not limit the scope of the present utility model. Further, various other changes and modifications will be apparent to those skilled in the art from the foregoing description. It is not necessary here nor is it exhaustive of all embodiments. And obvious variations or modifications thereof are contemplated as falling within the scope of the present utility model.

Claims

1. A nucleic acid amplification detection device comprising a housing (1) and a reaction tube (2), characterized in that: be equipped with cavity (101) and lower cavity (102) in casing (1), be equipped with on casing (1) with last cavity (101) intercommunication connecting cylinder (3), connecting tube (2) can dismantle and connect in connecting cylinder (3), and reaction tube (2) bottom is equipped with annular sealing membrane (4), connecting cylinder (3) bottom is equipped with awl post (5), be equipped with test paper (6) in last cavity (101), be equipped with puncture subassembly and observation window (7) respectively on the casing of connecting cylinder (3) both sides, be equipped with the through-hole on last cavity (101) diapire of puncture subassembly below, sealing connection has sealing membrane (8) in the through-hole.

2. The nucleic acid amplification detection apparatus according to claim 1, wherein: the utility model discloses a reaction tube, including connecting tube (2), connecting tube (3), connecting tube (5), connecting tube (2), connecting tube (3) bottom is equipped with inlet (9) with last cavity (101) intercommunication, be fixed with ejector pin (11) through support (10) in the middle of inlet (9), awl post (5) are fixed on support (10) and awl post (5) are located sealing membrane (4) below.

3. The nucleic acid amplification detection apparatus according to claim 1, wherein: an elastic sealing ring (12) is arranged at the upper end of the connecting cylinder (3).

4. The nucleic acid amplification detection apparatus according to claim 1, wherein: the puncture assembly comprises an elastic sealing sleeve (13), a conical strip (14) and a spring (15), wherein a mounting groove is formed in a shell (1) on one side of the connecting cylinder (3), a jack (16) communicated with the upper cavity (101) is formed in the bottom of the mounting groove, the lower end of the sealing sleeve (13) is connected in the mounting groove in a sealing mode, the conical strip (14) is movably inserted into the jack (16), a pressing portion (17) is arranged at the upper end of the conical strip (14), and the spring (15) is sleeved outside the conical strip (14) between the pressing portion (17) and the bottom surface of the mounting groove.

5. The nucleic acid amplification detection apparatus according to claim 1, wherein: a drainage rod (18) is fixedly connected in the through hole below the sealing film (8).