CN112226352A

CN112226352A - Multifunctional gene detection device

Info

Publication number: CN112226352A
Application number: CN202011198579.9A
Authority: CN
Inventors: 不公告发明人
Original assignee: Qin Zeying
Current assignee: Qin Zeying
Priority date: 2020-10-31
Filing date: 2020-10-31
Publication date: 2021-01-15

Abstract

The invention relates to the technical field of medical detection, in particular to a multifunctional gene detection device which comprises a support stand and a main cylinder arranged in the support stand, wherein a sealing top cover is fixed at the top of the main cylinder, a push cylinder is arranged on the sealing top cover, a sealing plug is arranged at the top of the push cylinder, a connecting cylinder is fixed at the bottom of the main cylinder, a support ring is fixed in the middle of the outer wall of the connecting cylinder, and a limit handle is fixed at the top of the sealing plug and at one end of an adjusting screw rod, which is far away from; the outer wall of the push cylinder is provided with scales, when the height difference between the top height of the push cylinder and the top of the sealing top cover is 10CM, the first bayonet penetrates through the isolation film, when the top height of the push cylinder and the top height of the sealing top cover are parallel and level, the isolation film completely penetrates to disappear, and the distance between the bottom of the first bayonet and the top of the filtering mechanism is smaller than 1 CM. The portable multifunctional electric scooter has the advantages of simple structure, easiness in carrying and good market prospect.

Description

Multifunctional gene detection device

Technical Field

The invention relates to the technical field of medical detection, in particular to a multifunctional gene detection device.

Background

The public health impact and awareness of modern medicine on environmental stores of infectious and emerging diseases, bio-threat agents, genetic diseases and disease causing agents has increased, and thus the need for more informative, sensitive and specific rapid assays to be used has increased the demand for sample detection tools.

At present, nucleic acid is extracted by a conventional nucleic acid extraction method, and molecular detection by a PCR amplification method is very sensitive, specific and information. Unfortunately, currently available methods of extracting nucleic acids and nucleic acid detection are not suitable for use at or have limited utility at the sampling site because they require elaborate, bulky and expensive instruments, specialized laboratory materials, and/or multiple operations that rely on user intervention. Therefore, most samples for molecular detection are shipped to a centralized laboratory, which results in long turnaround times to obtain the required information.

The prior art devices for detection are very expensive and contamination may still be present as many samples will be processed by the same device. In summary, the diagnosis of nucleic acid amplification by extraction is greatly limited due to the large-scale apparatus required in the detection method of the prior art and the defect that the amplification product is easily contaminated. Therefore, a multifunctional gene detection device is provided.

Disclosure of Invention

The present invention is directed to a multifunctional gene testing device to solve the above problems of the prior art.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a multi-functional gene detection device, includes pallet (2) and installs main drum (1) inside it, its characterized in that: a sealing top cover (14) is fixed at the top of the main cylinder (1), a push cylinder (15) is installed on the sealing top cover (14), and a sealing plug (16) is installed at the top of the push cylinder (15); a connecting cylinder (9) is fixed at the bottom of the main cylinder (1), a support ring (13) is fixed on the outer wall of the connecting cylinder (9), an elastic annular wall (10) is hermetically connected at the bottom of the connecting cylinder (9), and a detection cylinder (11) is fixed at the bottom of the elastic annular wall (10);

a supporting block (3) is fixed inside the support table (2), a ring embedding groove (4) matched with the supporting ring (13) is formed in the middle of the supporting block (3), and a channel (5) is formed in the bottom of the support table (2); longitudinal channels (202) are formed in the left side and the right side of the support table (2), sliding seats (6) are connected to the outer walls of the left side and the right side of the support table (2) in a sliding mode, and adjusting screws (7) are connected to the sliding seats (6) in a threaded mode; one opposite sides of the two adjusting screws (7) extend into the support table (2) through the longitudinal channel (202) and are fixedly provided with heating rings (8), and the two heating rings (8) are folded and sleeved on the outer wall of the main cylinder (1);

an electric telescopic rod (18) is arranged below the support platform (2), the bottom of the detection cylinder (11) extends to the position below the support platform (2) through the channel (5), and a supporting plate (17) matched with the detection cylinder (11) is fixed at the top of the electric telescopic rod (18); a piston plate (20) is fixed inside the main cylinder (1) and extends to the bottom of the push cylinder (15), a first bayonet knife (21) is installed in the middle of the bottom of the push cylinder (15), and the bottom of the first bayonet knife (21) extends to the position below the piston plate (20); a stainless steel pipe (151) is arranged at the bottom of the push cylinder (15), and the bottom of the stainless steel pipe (151) penetrates through the outer surface of the first bayonet (21); an isolating membrane (22) and a filtering mechanism (23) are sequentially arranged in the main cylinder (1) from top to bottom, a dry cracking reagent, a protease K, carrierRNA and other nucleic acid extraction reagents for extracting nucleic acid are attached to the isolating membrane (22), a partition plate (12) is fixed at the top of the detection cylinder (11), a second bayonet (25) is arranged on one side of the top of the partition plate (12), a water absorbing paper (24) is arranged in the middle of the second bayonet (25), and the bottom of the water absorbing paper (24) extends to the inner cavity of the detection cylinder (11); the inner cavity of the detection cylinder (11) is sequentially provided with a sample pad (26), a colored particle combination pad (27), a fiber membrane (28), a detection line (29), a quality control line (30) and a water absorption filter paper pad (31) from top to bottom, and the parts of the sample pad, the colored particle combination pad, the fiber membrane, the detection line, the quality control line and the water absorption filter paper pad are overlapped at adjacent parts;

the main cylinder (1) extracts and amplifies nucleic acid and then detects in the detection cylinder (11), the inner space of the main cylinder (1) is divided into three chambers by the isolating membrane (22) and the filtering mechanism (23), and the three chambers are independent in function, are sequential and compact; the cavity at the bottom of the main cylinder (1) is attached with frozen dry powder of all components required by amplification reaction, and the isolation membrane (22) is closed and can be punctured by the first puncture knife (21);

the heating ring (8) is externally connected with an external power supply through a wire, the heating ring (8) can be freely sleeved on the outer wall of the main cylinder (1), long sliding grooves (19) are formed in the outer walls of the left side and the right side of the support table (2), and the long sliding grooves (19) and the longitudinal channel (202) are consistent in length and are parallel to each other; a T-shaped sliding block matched with the long sliding groove (19) is fixed at one end, close to the outer wall of the support table (2), of the sliding seat (6), and a first threaded hole matched with the adjusting screw rod (7) is formed in the sliding seat (6); limiting handles are fixed on the top of the sealing plug (16) and one end of the adjusting screw rod (7) far away from the main cylinder (1); the outer wall of the push cylinder (15) is provided with scales, when the height difference between the top of the push cylinder (15) and the top of the sealing top cover (14) is 10CM, the first bayonet (21) penetrates through the isolation film (22), when the top of the push cylinder (15) is flush with the top of the sealing top cover (14), the isolation film (22) is completely penetrated to disappear, and the distance between the bottom of the first bayonet (21) and the top of the filtering mechanism (23) is less than 1 CM.

Preferably, the bottom end of the second bayonet (25) is hollowed and connected with a water absorption paper (24), and the bottom of the water absorption paper (24) extends to the inside of the detection cylinder (11) and is connected with the sample pad (26).

Preferably, supporting legs (201) are installed at four corners of the bottom of the support platform (2), and buffering foot pads are fixed at the bottoms of the supporting legs (201).

Preferably, the supporting block (3) and the supporting ring (13) are equidistantly provided with mounting holes which are matched with each other, and the supporting block (3) and the support table (2) are integrally connected and formed.

Preferably, the main cylinder (1) and the detection cylinder (11) are both made of transparent materials, and the elastic annular wall (10) is made of medical silicon rubber.

Compared with the prior art, the invention has the beneficial effects that:

1. the slide seat is arranged to move inside the long slide groove through the T-shaped slide block, so that the adjusting screw rod is driven to move up and down, the height position of the heating rings is adjusted, the adjusting screw rod is in threaded connection with the slide seat, the adjusting screw rod is rotated, the distance between the two heating rings is adjusted, when the two heating rings are folded and tightly sleeved on the outer wall of the main cylinder, the resistance between the heating rings and the outer wall of the main cylinder prevents the heating rings from sliding downwards, the operation is easy, samples are incubated through the heating rings, and the advantages of rapidness, high efficiency and the like are achieved; the heating ring can move up and down, can be separated and folded, and can carry out incubation on different positions of the main cylinder;

2. according to the invention, the elastic ring wall is compressed through the electric telescopic rod, so that the top of the second bayonet is penetrated into the main cylinder, liquid enters the detection cylinder, when the sample pad absorbs enough liquid, the liquid enters the colored particle combination pad and the fibrous membrane under the capillary action, the colored particles on the colored particle combination pad are coated with the anti-A antibody, the detection line is coated with the anti-B antibody, and the quality control line is coated with the anti-A antibody;

3. the main cylinder and the detection cylinder are disposable articles with good tightness, the possibility of pollution is eliminated, the structure is simple, the carrying is easy, and the method can be applied to all fields needing to use a nucleic acid amplification detection technology method for detection.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic view of a first perspective structure of the present invention;

FIG. 2 is a schematic view of a second perspective structure of the present invention;

FIG. 3 is a front view of the present invention;

FIG. 4 is a side view of the present invention;

FIG. 5 is a schematic structural view of a support stand according to the present invention;

FIG. 6 is a schematic view of the internal structure of the main cylinder and the detection cylinder according to the present invention;

FIG. 7 is a schematic view of the main cylinder with a first lancet piercing the isolation diaphragm;

FIG. 8 is a schematic view of the first bayonet of the present invention inside the main cylinder, adjacent the filter mechanism;

FIG. 9 is a schematic view of the internal structure of the main cylinder according to the present invention;

FIG. 10 is a schematic view of the structure of the present invention at A.

In the figure: 1. a main cylinder; 2. a support stand; 201. a support leg; 202. a longitudinal channel; 3. a support block; 4. a ring caulking groove; 5. a channel; 6. a slide base; 7. adjusting the screw rod; 8. a heating ring; 9. a connecting cylinder; 10. an elastic annular wall; 11. detecting the cylinder; 12. a partition plate; 13. a support ring; 14. sealing the top cover; 15. a push cylinder; 151. a stainless steel tube; 16. a sealing plug; 17. a support plate; 18. an electric telescopic rod; 19. a long chute; 20. a piston plate; 21. a first bayonet; 22. an isolation film; 23. a filtering mechanism; 24. absorbent paper; 25. a second bayonet; 26. a sample pad; 27. a colored particulate conjugate pad; 28. a fibrous membrane; 29. detecting lines; 30. a quality control line; 31. a water absorbing filter paper pad.

Detailed Description

In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present application will be described in detail with reference to the accompanying drawings 1 to 10, in conjunction with the embodiments.

Referring to fig. 1 and 3, a multifunctional gene testing device comprises a support stand 2 and a main cylinder 1 arranged inside the support stand, wherein a sealing top cover 14 is fixed on the top of the main cylinder 1, a push cylinder 15 is arranged on the sealing top cover 14, a sealing plug 16 is arranged on the top of the push cylinder 15, when in testing, the sealing plug 16 is opened to pour a sample into the push cylinder 15, then the sample enters the main cylinder 1 through a stainless steel pipe 151, a separation membrane 22 is arranged to isolate the sample from a cavity on the top of the main cylinder 1, and the extracted sample can be whole blood, sputum, urine, feces, a part of tissue, an organ or any other source containing a target nucleic acid sequence;

a connecting cylinder 9 is fixed at the bottom of the main cylinder 1, a support ring 13 is fixed in the middle of the outer wall of the connecting cylinder 9, an elastic annular wall 10 is connected at the bottom of the connecting cylinder 9 in a sealing manner, a detection cylinder 11 is fixed at the bottom of the elastic annular wall 10, a support block 3 is fixed in the support table 2, a ring caulking groove 4 matched with the support ring 13 is arranged in the middle of the support block 3, a channel 5 is arranged at the bottom of the support table 2, longitudinal channels 202 are arranged at the left side and the right side of the support table 2, sliding seats 6 are connected on the outer walls of the left side and the right side of the support table 2 in a sliding manner, adjusting screws 7 are connected on the sliding seats 6 in a threaded manner, opposite sides of the two adjusting screws 7 extend into the support table 2 through the longitudinal channels 202 and are fixed with heating rings 8, the two heating rings 8 are folded and sleeved on the outer wall of the main cylinder, the bottom of the detection cylinder 11 extends to the lower part of the support platform 2 through the channel 5, and the top of the electric telescopic rod 18 is fixed with a supporting plate 17 matched with the detection cylinder 11;

as shown in fig. 5 to 10, a piston plate 20 is fixed inside the main cylinder 1 and extends from the bottom of the push cylinder 15, a first bayonet 21 is installed in the middle of the bottom of the push cylinder 15, the bottom of the first bayonet 21 extends below the piston plate 20, a stainless steel tube 151 is disposed at the bottom of the push cylinder 15, the bottom of the stainless steel tube 151 penetrates through the outer surface of the first bayonet 21, an isolation membrane 22 and a filtering mechanism 23 are sequentially disposed inside the main cylinder 1 from top to bottom, the filtering mechanism 23 can remove all potential inhibitors, small molecules, ions and proteins, a nucleic acid extraction reagent for extracting nucleic acid such as a dry cracking reagent and a protease K, carrierRNA is attached to the isolation membrane 22, a partition plate 12 is fixed at the top of the detection cylinder 11, a second bayonet 25 is disposed on one side of the top of the partition plate 12, a water absorption paper 24 is disposed in the middle of the second bayonet 25, and the bottom of the water absorption paper 24 extends to the inner cavity of the detection cylinder 11, the detection cylinder 11 is internally provided with a sample pad 26, a colored particle combination pad 27, a fiber membrane 28, a detection line 29, a quality control line 30 and a water absorption filter paper pad 31 from top to bottom in sequence, the adjacent parts of the sample pad, the colored particle combination pad 27, the fiber membrane 28, the detection line 29, the quality control line 30 and the water absorption filter paper pad 31 are partially overlapped, nucleic acid is extracted and amplified in the main cylinder 1, and then the detection is carried out in the detection cylinder 11, the internal space of the main cylinder 1 is divided into three chambers through the isolating membrane 22 and the filtering mechanism 23, and the three chambers are functionally independent and are sequentially; the cavity at the bottom of the main cylinder 1 is attached with all frozen dry powder required by amplification reaction, the isolation membrane 22 is closed and can be punctured by the first bayonet 21, the distance between the isolation membrane 22 and the sealing top cover 14 is the same as the length of the first bayonet 21, the nucleic acid detection test strip is an improvement on the well-established immunochromatography color development technology, the amplified detection product is combined with the colloidal gold particles by applying the nano-colloidal gold particles or other colored particles coated with specific nucleic acid or protein and specially marked nucleic acid primers and probes, and the DNA amplification product is detected by developing on the detection line T line of the test strip; if the target DNA is not available, the specific probe can not connect the amplified product with the marker, and the result has no color development, namely, the result is read as negative;

as shown in fig. 1-3, the heating ring 8 is externally connected with an external power supply through a wire, the heating ring 8 can be freely sleeved on the outer wall of the main cylinder 1, the temperature of the heating ring 8 is regulated, the outer walls of the left and right sides of the support table 2 are provided with long sliding grooves 19, the long sliding grooves 19 and the longitudinal channel 202 have the same length and are parallel to each other, one end of the sliding base 6 close to the outer wall of the support table 2 is fixed with a T-shaped sliding block matched with the long sliding grooves 19, the sliding base 6 is provided with a first threaded hole matched with the adjusting screw 7, the sliding base 6 moves inside the long sliding grooves 19 through the T-shaped sliding block, so as to drive the adjusting screw 7 to move up and down, thereby regulating the height position of the heating ring 8, the adjusting screw 7 is in threaded connection with the sliding base 6, and the adjusting screw 7 is rotated, so that the adjusting, therefore, the distance between the two heating rings 8 is adjusted, when the two heating rings 8 are folded and tightly sleeved on the outer wall of the main cylinder 1, the resistance between the heating rings 8 and the outer wall of the main cylinder 1 prevents the heating rings 8 from sliding downwards, and the structure is simple and easy to operate;

as shown in fig. 1-4, a limiting handle is fixed at both the top of the sealing plug 16 and one end of the adjusting screw 7 away from the main cylinder 1, a second threaded hole matched with the push cylinder 15 is formed in the middle of the sealing top cover 14, a rubber column is fixed inside the push cylinder 15 extending from the bottom of the sealing plug 16, the outer diameter of the rubber column is in interference fit with the inner diameter of the push cylinder 15, support legs 201 are installed at four corners of the bottom of the support stand 2, buffer foot pads are fixed at the bottoms of the support legs 201, mounting holes matched with each other are formed at equal distances on the support block 3 and the support ring 13, the support block 3 and the support stand 2 are integrally connected and formed, the main cylinder 1 and the detection cylinder 11 are made of transparent materials, the elastic ring wall 10 is made of medical silicon rubber, and is good in corrosion resistance and elasticity, when in use, the main cylinder 1 is placed inside the support stand 2, so that the support ring 13 enters the ring caulking groove 4, and then the main cylinder 1 is stably installed inside the support stand 2 by penetrating and connecting the support block 3 and the mounting hole on the support ring 13 through the bolt.

As shown in fig. 5 to 10, when the electric telescopic rod 18 is extended, the detecting cylinder 11 is compressed, so that the elastic annular wall 10 is compressed, the second bayonet 25 pierces the chamber at the bottom of the main cylinder 1, the seal cap 14 can be an activated carbon filter, an ion exchange filter, or a semi-permeable membrane with a molecular cut-off range of 300000D-400000D, and they are non-adhesive coated, the bottom end of the second bayonet 25 is hollowed and connected with a water absorption paper 24, the bottom of the water absorption paper 24 extends to the inside of the detecting cylinder 11 and is connected with the sample pad 26, the outer wall of the push cylinder 15 is provided with scales, when the height difference between the top of the push cylinder 15 and the top of the seal cap 14 is 10CM, the first bayonet 21 penetrates through the isolating membrane 22, when the top of the push cylinder 15 is level with the top of the seal cap 14, the isolating film 22 is penetrated completely to disappear, and the distance between the bottom of the first bayonet 21 and the top of the filter mechanism 23 is less than 1 CM.

During detection, the sealing plug 16 is pulled out from the push cylinder 15, a sample is added into the push cylinder 15, the sample is placed at the top of the isolation film 22, then the sealing plug 16 seals the push cylinder 15, the sliding seat 6 moves up and down on the support platform 2 to drive the heating ring 8 to move on the outer wall of the main cylinder 1, the position of the heating ring 8 is flush with the position of the isolation film 22, after the heating ring 8 is incubated for a period of time, the sealing plug 16 is rotated clockwise to drive the push cylinder 15 to move downwards until the height difference between the top height of the push cylinder 15 and the top of the sealing top cover 14 is 10CM, as shown in fig. 6, the first bayonet 21 punctures the isolation film 22, after all liquid flows into the filtering mechanism 23, the liquid finally enters the bottom of the main cylinder 1, the sealing plug 16 continues to rotate clockwise until the top height of the push cylinder 15 is flush with the top height of the sealing top cover 14, the adjusting, the two adjusting screws 7 are enabled to move towards or away from each other, when the two adjusting screws 7 move away from each other, the two heating rings 8 move away from each other, the heating rings 8 naturally slide down under the influence of natural gravity, the heating rings 8 are enabled to be flush with the cavity at the bottom of the main cylinder 1, then the adjusting screws 7 are rotated in the same direction, the two adjusting screws 7 move towards or away from each other, when the two adjusting screws 7 move towards each other, the two heating rings 8 are enabled to be folded and tightly enclosed on the outer wall of the main cylinder 1, the heating rings 8 cannot fall off, the temperature of the heating rings 8 is adjusted, after the liquid in the cavity at the bottom of the main cylinder 1 is incubated for a period of time, the electric telescopic rod 18 is opened, the electric telescopic rod 18 is enabled to extend, the supporting plate 17 compresses the detection cylinder 11 upwards, the elastic ring wall 10 is enabled to shrink, as shown in figure 10, at this time, the top of the second bayonet, when the sample pad 26 absorbs enough liquid, the liquid enters the colored particle bonding pad 27 and the fiber membrane 28 under the action of capillary, the colored particles on the colored particle bonding pad 27 are coated with the anti-A antibody, the detection line 29 is coated with the anti-B antibody, the quality control line 30 is coated with the anti-A antibody, the colored particles are colloidal gold particles or latex particles, the fiber membrane is usually a nitrocellulose membrane or a nylon membrane, and an observable line is formed if the target sequence exists.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a multi-functional gene detection device, includes pallet (2) and installs main drum (1) inside it, its characterized in that: a sealing top cover (14) is fixed at the top of the main cylinder (1), a push cylinder (15) is installed on the sealing top cover (14), and a sealing plug (16) is installed at the top of the push cylinder (15); a connecting cylinder (9) is fixed at the bottom of the main cylinder (1), a support ring (13) is fixed on the outer wall of the connecting cylinder (9), an elastic annular wall (10) is hermetically connected at the bottom of the connecting cylinder (9), and a detection cylinder (11) is fixed at the bottom of the elastic annular wall (10);

2. The multifunctional gene assaying device according to claim 1, wherein: the bottom end of the second bayonet (25) is hollowed and connected with absorbent paper (24), and the bottom of the absorbent paper (24) extends into the detection cylinder (11) and is connected with the sample pad (26).

3. The multifunctional gene assaying device according to claim 1, wherein: support legs (201) are all installed to support frame (2) bottom four corners department, all be fixed with buffering callus on the sole bottom support legs (201).

4. The multifunctional gene assaying device according to claim 1, wherein: the supporting block (3) and the supporting ring (13) are equidistantly provided with mounting holes which are matched with each other, and the supporting block (3) and the support table (2) are integrally connected and formed.

5. The multifunctional gene assaying device according to claim 1, wherein: the main cylinder (1) and the detection cylinder (11) are made of transparent materials, and the elastic annular wall (10) is made of medical silicon rubber.