CN114088498B - Pretreatment device for biological sample and application thereof - Google Patents
Pretreatment device for biological sample and application thereof Download PDFInfo
- Publication number
- CN114088498B CN114088498B CN202111524715.3A CN202111524715A CN114088498B CN 114088498 B CN114088498 B CN 114088498B CN 202111524715 A CN202111524715 A CN 202111524715A CN 114088498 B CN114088498 B CN 114088498B
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- push rod
- unit
- main reaction
- reaction chamber
- chamber
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- 239000012472 biological sample Substances 0.000 title claims abstract description 40
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 11
- 238000007789 sealing Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 description 13
- 239000003085 diluting agent Substances 0.000 description 9
- 230000006037 cell lysis Effects 0.000 description 6
- 239000006166 lysate Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 239000011358 absorbing material Substances 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The invention provides a pretreatment device for biological samples, which comprises a push rod unit, a reaction unit and a needle head unit, wherein the reaction unit is provided with a plurality of chambers, a main reaction chamber bears the biological samples to be treated, reagents required for treating the biological samples are stored in a side chamber, a connecting hole is arranged between the main reaction chamber and the side chamber, the reagents in the side chamber are sequentially pushed into the main reaction chamber through a push rod of the push rod unit, and the main reaction chamber is connected with the needle head unit. The pretreatment device for the biological sample has a simple structure, can be used in a scene separated from a laboratory, and is quick and convenient to use.
Description
Technical Field
The invention belongs to the field of biomedical detection, and particularly relates to a pretreatment device for a biological sample and application thereof.
Background
The prior biological sample pretreatment steps such as cell lysis and the like need to be utilized in a laboratory; at present, the nucleic acid extraction process which comprises the steps of cell lysis, nucleic acid purification and the like and meets the PCR detection needs to be completed in a laboratory, and the whole process needs to use various experimental instruments such as a centrifugal machine and the like, so that the operation steps are more, the operation is complicated, the time is long, and the requirement of rapid detection cannot be met.
Therefore, there is an urgent need for a pretreatment device for biological samples, which can be portable, convenient, and can rapidly replace the reaction chamber.
Disclosure of Invention
In order to overcome the problems in the prior art, the invention provides the pretreatment device for the biological sample and the application method thereof, which can be conveniently carried, meet the measurement needs at any time and any place, quickly replace a reaction chamber, effectively enrich the biological sample to be reacted and provide reliability for subsequent experiments.
The invention provides the following technical scheme:
The utility model provides a biological sample's preprocessing device, includes push rod unit, reaction unit and syringe needle unit, the reaction unit is equipped with a plurality of cavities, and main reaction chamber bears the biological sample that waits to process, stores the required reagent of handling the biological sample in the side cavity, is equipped with the connecting hole between main reaction chamber and the side cavity, pushes the main reaction chamber with the reagent in the side cavity in proper order through the push rod of push rod unit, and main reaction chamber connects the syringe needle unit.
Further, a secondary push rod is arranged on the upper portion of the main reaction chamber, a return spring is arranged on the secondary push rod, a rubber plug is arranged at the lower end of the secondary push rod, a sampler is arranged at the downstream of the secondary push rod, and the sampler adsorbs liquid of a biological sample to be processed.
Further, the upper part and the lower part of the side chamber are respectively sealed by rubber plugs, the connecting holes between the side chamber and the main reaction chamber are also sealed by the rubber plugs, and the push rod can push the sealing rubber plugs.
Further, the needle unit is connected to the downstream of the sampler and comprises a rubber plug, a needle connector and a needle, the upper end of the needle connector is connected with the rubber plug and connected with the main reaction chamber, and the needle penetrates through the rubber plug and extends out of the lower end of the needle connector.
Further, the bottom of the side chamber is also provided with ventilation holes.
Further, the push rod units are provided with push rods corresponding to the chambers one by one, and return springs are arranged on the push rods.
Further, the push rod unit is connected with the push rod through a rotary rod, a knob is arranged at the top of the rotary rod, the rotary rod is driven to rotate by the knob, the push rod is rotated to the upper parts of different chambers, and a return spring is arranged on the push rod.
Further, the shell inner wall of the push rod unit is provided with a closed-loop slideway, the rotary rod is connected with the push rod, a knob is arranged at the top of the rotary rod, an inverted triangle slide block is arranged in the slideway, the slide block is connected with the rotary rod, and the joint of the horizontal slideway and the vertical slideway is provided with a corner, so that the slide block can only enter the next horizontal slideway, and a return spring is arranged on the push rod.
Further, the push rod unit, the reaction unit and the needle unit are detachably connected.
A method for extracting nucleic acid from eukaryotic cells using a pretreatment device for a biological sample, comprising the steps of:
Step one, a sampler for adsorbing cell liquid is placed in a main reaction chamber,
Step two, pressing a push rod corresponding to the main reaction chamber, pushing the sponge by the secondary push rod to be compressed, enabling redundant liquid to flow out from the needle head, enriching cells to be extracted on the sampler, enabling the secondary push rod to move upwards under the action of the return spring,
Step three, pressing a push rod corresponding to a side chamber for storing the lysate, moving a rubber plug at the lower part of the side chamber for storing the lysate downwards, exposing a connecting hole between the side chamber and the main reaction chamber, enabling the lysate to enter the main reaction chamber, completing cell lysis, relieving the pressure of the push rod, moving the push rod upwards under the action of a return spring, and moving the rubber plug upwards to block the connecting hole again;
Step four, pressing a push rod corresponding to a side chamber for storing the diluent, moving a rubber plug at the lower part of the side chamber for storing the diluent downwards, exposing a connecting hole between the side chamber and the main reaction chamber, enabling the diluent to enter the main reaction chamber to complete dilution of cell lysis, releasing the pressure of the push rod, moving the push rod upwards under the action of a return spring, and moving the rubber plug upwards to block the connecting hole again;
Step five, pressing a push rod corresponding to the main reaction chamber, and enabling the lysis diluent in the main reaction chamber to flow out of the needle head.
By adopting the technical scheme, the invention has the following beneficial effects:
(1) The pretreatment device for the biological sample has a simple structure, can be used in a scene separated from a laboratory, and is quick and convenient to use.
(2) The push rod unit, the reaction unit and the needle unit of the pretreatment device for biological samples can be mutually independent, and the reaction unit is replaced to realize multiple uses.
(3) The reaction unit of the biological sample is provided with the secondary push rod and the sponge structure, so that enrichment of the target biological sample can be realized, and the reliability of the experiment is improved.
Drawings
FIG. 1 is a schematic structural view of a pretreatment device for biological samples according to the present invention;
FIG. 2 is a schematic structural view of a main reaction chamber of the present invention;
FIG. 3 is a schematic view of the structure of the side chamber of the present invention;
FIG. 4 is a schematic view of the structure of an embodiment of the putter unit of the present invention;
FIG. 5 is a schematic view of another embodiment of the putter unit of the present invention;
FIG. 6 is a side exploded view of a housing of another embodiment of the putter unit of the present invention;
Fig. 7 is an enlarged view of a side-developed structure of a housing of another embodiment of the putter unit of the present invention.
Wherein: 1-push rod unit, 11-push rod, 12-knob, 13-rotary rod, 14-slideway and 15-slide block; the device comprises a 2-reaction unit, a 21-main reaction chamber, a 22-side chamber, a 23-connecting hole, a 24-rubber plug, a 25-ventilation hole, a 26-sampler and a 27-secondary push rod; 3-needle unit, 31-rubber plug, 32-needle connector, 33-needle.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the drawings and detailed description are only intended to illustrate the invention and are not intended to limit the invention.
Example 1
As shown in fig. 1 to 3, the present invention provides a pretreatment device for biological samples, comprising a push rod unit 1, a reaction unit 2 and a needle unit 3, wherein the reaction unit is provided with a plurality of chambers, a main reaction chamber 21 carries the biological sample to be treated, a side chamber 22 stores reagents required for treating the biological sample, a connecting hole 23 is arranged between the main reaction chamber and the side chamber, the reagents in the side chamber are sequentially pushed into the main reaction chamber through a push rod 11 of the push rod unit, and the main reaction chamber is connected with the needle unit. The biological sample may be a human cell, an animal cell, a virus, a eukaryotic cell or a microorganism.
The reaction chamber is used for processing biological samples, the connecting rod unit is used for controlling the opening of connecting holes among different chambers, so that reagent mixing among different chambers is controlled, and meanwhile, the reaction liquid in the main reaction chamber can be controlled to flow out of the needle head unit, so that the pretreatment work of the biological samples is completed.
Preferably, the upper part of the main reaction chamber is provided with a secondary push rod 27, a return spring is arranged on the secondary push rod, a sampler 26 is arranged at the downstream of the secondary push rod, a rubber plug at the lower end of the secondary push rod plays a role in sealing, the sampler adsorbs liquid of a biological sample to be treated, and the sampler can be made of water-absorbing materials such as sponge.
The secondary push rod is coaxially arranged with the push rod corresponding to the main reaction chamber, when the push rod corresponding to the main reaction chamber is pressed, the secondary push rod and the push rod move downwards together, the rubber plug at the lower end of the secondary push rod extrudes the sampler to deform, after the pressing is relieved, the push rod corresponding to the main reaction chamber moves upwards, the secondary push rod moves upwards under the action of the return spring, and the sampler is restored to the original state. In this way, the sampler can hold the biological sample, and the liquid in the sampler flows out of the needle unit, so that enrichment of the biological sample to be treated is achieved.
The needle unit is connected to the downstream of the sampler and comprises a rubber plug 31, a needle connector 32 and a needle 33, the upper end of the needle connector is connected with the rubber plug and is connected with the main reaction chamber, the lower end of the main chamber can be in a conical shape with the upper part narrow and the lower part wide, the rubber plug is in the same shape, and the rubber plug plays a role in sealing. The needle penetrates through the rubber plug and extends out of the lower end of the needle connector, so that liquid in the main reaction chamber can be extruded from the needle.
Preferably, the push rod unit, the reaction unit and the needle unit are detachably connected, the connection mode can be clamping connection or threaded connection, so that the reaction unit and the needle unit can be replaced, multiple detection is facilitated, the reaction unit adds a sampler for soaking biological sample liquid to be treated into the main reaction chamber before use, the needle unit is connected with the reaction unit, and a rubber plug at the upper end of the needle connector is extruded with the side surface of the chamber to form sealing.
Example 2
The upper and lower parts of the side chamber 22 are respectively sealed by rubber plugs 24, and the connecting holes 23 between the side chamber and the main reaction chamber are also sealed by rubber plugs, so that the push rod 11 can push the sealing rubber plugs to move downwards, and the lower rubber plugs move downwards to expose the connecting holes, so that pre-stored liquid enters the main reaction chamber. After all pre-stored liquid is extruded, the upper rubber plug reaches the connecting hole, and the connecting hole can be sealed again to prevent liquid from flowing back.
The bottom of the side chamber is also provided with an air hole for exhausting the air between the lower rubber plug and the chamber, so that the rubber plug can smoothly move in the chamber.
In this embodiment, the pusher units may be provided as pusher rods corresponding to the chambers one by one, and the pusher rods are provided with return springs. And sequentially pressing the push rod according to experimental steps to finish liquid extrusion of different chambers.
Example 3
The push rod unit can adopt the structure as shown in fig. 4, and the push rod unit is connected with push rod 11 by rotary rod 13, is equipped with knob 12 at the rotary rod top, and the knob drives the rotary rod rotation, rotates the push rod to different cavity top, is equipped with return spring on the push rod. The corresponding positions of the push rod and the chambers are adjusted by rotating the knob, so that liquid extrusion of different chambers is controlled. The single push rod avoids the space clamping between the three push rods, can fully design the push rod more comfortably, and is smooth to use. Meanwhile, the single push rod can reduce the complexity of the inside of the push rod unit, reduce spare parts and reduce cost, and simultaneously release more space.
In addition, the push rod unit can also adopt the structure as shown in fig. 5 and 6, a closed-loop slideway 14 is arranged on the inner wall of the shell of the push rod unit, a rotary rod is connected with the push rod 11, a knob 12 is arranged at the top of the rotary rod, an inverted triangle slide block 15 is arranged in the slideway, the slide block is connected with the rotary rod, the rotary knob drives the slide block to slide in the slideway, the slide block slides in different transverse slideways to bring the push rod to the upper parts of different chambers, the slide block slides in the longitudinal direction, and the push rod moves up and down. The corner is arranged at the joint of the horizontal slide way and the vertical slide way, so that the sliding block can only enter the next horizontal slide way, and the push rod is provided with a return spring. The corner may be configured as an angled sidewall with a lower width of the corner being less than a width of the top surface of the inverted triangle such that the slider can only move laterally after passing through the corner. The slide way strictly limits the moving track of the slide block, so that the position of the push rod can be precisely limited, and the push rod can be aligned with the chamber according to a preset sequence.
Example 4
The invention provides a method for extracting eukaryotic cell nucleic acid by using a pretreatment device of a biological sample, which comprises the following steps:
step one, a sampler for adsorbing the cell fluid is placed in a main reaction chamber.
Step two, pressing a push rod corresponding to the main reaction chamber, pushing the sponge by the secondary push rod to be compressed, enabling redundant liquid to flow out from the needle head, enriching cells to be extracted on the sampler, and enabling the secondary push rod to move upwards under the action of the return spring.
Step three, pressing a push rod corresponding to a side chamber for storing the lysate, moving a rubber plug at the lower part of the side chamber for storing the lysate downwards, exposing a connecting hole between the side chamber and the main reaction chamber, enabling the lysate to enter the main reaction chamber, completing cell lysis, relieving the pressure of the push rod, moving the push rod upwards under the action of a return spring, and moving the rubber plug upwards to block the connecting hole again.
And step four, pressing a push rod corresponding to a side chamber for storing the diluent, moving a rubber plug at the lower part of the side chamber for storing the diluent downwards, moving the diluent downwards, exposing a connecting hole between the side chamber and the main reaction chamber, enabling the diluent to enter the main reaction chamber, completing the dilution of cell lysis, relieving the pressure of the push rod, moving the push rod upwards under the action of a return spring, and moving the rubber plug upwards to block the connecting hole again.
Step five, pressing a push rod corresponding to the main reaction chamber, and enabling the lysis diluent in the main reaction chamber to flow out of the needle head.
The foregoing examples merely illustrate embodiments of the invention and are described in more detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (4)
1. The pretreatment device for the biological sample comprises a push rod unit, a reaction unit and a needle head unit, and is characterized in that the reaction unit is provided with a plurality of chambers, a main reaction chamber bears the biological sample to be treated, reagents required for treating the biological sample are stored in a side chamber, a connecting hole is formed between the main reaction chamber and the side chamber, the reagents in the side chamber are sequentially pushed into the main reaction chamber through a push rod of the push rod unit, and the main reaction chamber is connected with the needle head unit; the upper part and the lower part of the side chamber are respectively sealed by rubber plugs, the connecting holes between the side chamber and the main reaction chamber are also sealed by the rubber plugs, and the push rod can push the sealing rubber plugs; the bottom of the side chamber is also provided with an air vent;
The push rod unit is connected with a push rod through a rotary rod, a knob is arranged at the top of the rotary rod, the rotary rod is driven to rotate by the knob, the push rod is rotated to the upper parts of different chambers, and a return spring is arranged on the push rod.
2. The pretreatment device for the biological sample comprises a push rod unit, a reaction unit and a needle head unit, and is characterized in that the reaction unit is provided with a plurality of chambers, a main reaction chamber bears the biological sample to be treated, reagents required for treating the biological sample are stored in a side chamber, a connecting hole is formed between the main reaction chamber and the side chamber, the reagents in the side chamber are sequentially pushed into the main reaction chamber through a push rod of the push rod unit, and the main reaction chamber is connected with the needle head unit; the upper part and the lower part of the side chamber are respectively sealed by rubber plugs, the connecting holes between the side chamber and the main reaction chamber are also sealed by the rubber plugs, and the push rod can push the sealing rubber plugs; the bottom of the side chamber is also provided with an air vent;
the casing inner wall of push rod unit is equipped with closed loop slide, and the rotary rod is connected the push rod, is equipped with the knob at the rotary rod top, is equipped with the triangle-shaped slider in the slide, and the slider links to each other with the rotary rod, and horizontal perpendicular slide junction is equipped with the turning for the slider can only get into next horizontal slide, is equipped with return spring on the push rod.
3. The pretreatment device for biological samples according to claim 1 or 2, wherein the needle unit comprises a rubber stopper, a needle connector and a needle, the upper end of the needle connector is connected with the rubber stopper and connected with the main reaction chamber, and the needle penetrates through the rubber stopper and protrudes from the lower end of the needle connector.
4. A biological sample pretreatment apparatus according to claim 3, wherein the pusher unit, the reaction unit and the needle unit are detachably connected.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2021114706354 | 2021-12-03 | ||
| CN202111470635 | 2021-12-03 |
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| Publication Number | Publication Date |
|---|---|
| CN114088498A CN114088498A (en) | 2022-02-25 |
| CN114088498B true CN114088498B (en) | 2024-04-26 |
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| CN202111524715.3A Active CN114088498B (en) | 2021-12-03 | 2021-12-14 | Pretreatment device for biological sample and application thereof |
| CN202123170849.8U Active CN216717957U (en) | 2021-12-03 | 2021-12-14 | Pretreatment device for biological samples |
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| CN202123170849.8U Active CN216717957U (en) | 2021-12-03 | 2021-12-14 | Pretreatment device for biological samples |
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| CN114088498B (en) * | 2021-12-03 | 2024-04-26 | 北京理工亘舒科技有限公司 | Pretreatment device for biological sample and application thereof |
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| CN104673625A (en) * | 2015-02-13 | 2015-06-03 | 西安交通大学 | Automatic reaction device and method for pretreating cells |
| CN106581820A (en) * | 2017-01-22 | 2017-04-26 | 张丽丽 | Modified anesthetic syringe for multi-drug injection |
| CN109456880A (en) * | 2018-12-20 | 2019-03-12 | 中国检验检疫科学研究院 | Nucleic acid on-site rapidly extracting pipe and its application method |
| CN111286449A (en) * | 2020-04-01 | 2020-06-16 | 宁波艾捷康宁生物科技有限公司 | Integrated adding method and device for pretreatment reagent of biological sample |
| CN111548926A (en) * | 2020-02-28 | 2020-08-18 | 青岛英赛特生物科技有限公司 | Totally-enclosed nucleic acid extraction and detection integrated kit |
| CN113423493A (en) * | 2018-11-22 | 2021-09-21 | 容器研究室有限公司 | Container, device and method for mixing a plurality of substances |
| CN216717957U (en) * | 2021-12-03 | 2022-06-10 | 北京理工亘舒科技有限公司 | Pretreatment device for biological samples |
-
2021
- 2021-12-14 CN CN202111524715.3A patent/CN114088498B/en active Active
- 2021-12-14 CN CN202123170849.8U patent/CN216717957U/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673625A (en) * | 2015-02-13 | 2015-06-03 | 西安交通大学 | Automatic reaction device and method for pretreating cells |
| CN106581820A (en) * | 2017-01-22 | 2017-04-26 | 张丽丽 | Modified anesthetic syringe for multi-drug injection |
| CN113423493A (en) * | 2018-11-22 | 2021-09-21 | 容器研究室有限公司 | Container, device and method for mixing a plurality of substances |
| CN109456880A (en) * | 2018-12-20 | 2019-03-12 | 中国检验检疫科学研究院 | Nucleic acid on-site rapidly extracting pipe and its application method |
| CN111548926A (en) * | 2020-02-28 | 2020-08-18 | 青岛英赛特生物科技有限公司 | Totally-enclosed nucleic acid extraction and detection integrated kit |
| CN111286449A (en) * | 2020-04-01 | 2020-06-16 | 宁波艾捷康宁生物科技有限公司 | Integrated adding method and device for pretreatment reagent of biological sample |
| CN216717957U (en) * | 2021-12-03 | 2022-06-10 | 北京理工亘舒科技有限公司 | Pretreatment device for biological samples |
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| Publication number | Publication date |
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| CN216717957U (en) | 2022-06-10 |
| CN114088498A (en) | 2022-02-25 |
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