CN203128565U - Anaerobic bacterium culture bottle vessel - Google Patents

Abstract

The utility model relates to a bottle dish for cultivating anaerobic bacteria, which comprises an inner plug connected by a cover body and a column body, a rectangular thin box-shaped bottle body provided with a bottleneck, and a round cover. The interface between the bottle body and the bottleneck is S-shaped, and the bottle body is connected with a bottleneck through the bottleneck; the inner plug is provided in the bottleneck, and the round cap is provided on the inner plug ; The inner plug is provided with a matching envelope, which is connected to the center of the inner wall of the round cover. The utility model has the advantages of simple structure, convenient operation and space saving, can be directly placed under a microscope for observation and use, and can be widely used in the cultivation and observation of aerobic, anaerobic or facultative anaerobic microorganisms.

Description

A kind of anaerobic species culturing bottle ware

Technical field

The utility model relates to a kind of culturing bottle ware, relates in particular to a kind of anaerobic species culturing bottle ware.

Background technology

In the anaerobion cell of strictness, there are not superoxide dismutase activity and catalase activity, and these two kinds of enzymes are directly relevant with the metabolism of the derivative of intracellular oxygen and oxygen, therefore the oxygen of denier just can make the vital movement of anaerobion be suppressed in the environment, even causes its death.Heng Gaite anaerobic device-copper post deaerating type of cycles, its principle is: the oxygen that the gas of steel cylinder (as nitrogen, carbonic acid gas etc.) all contains trace exists, from the gas of steel cylinder during by the about 350 ℃ copper post of temperature, oxygen in copper and the gas reacts and is oxidized to cupric oxide, thereby reaches the purpose of oxygen in the Purge gas; The blackening after oxidation of copper post can generate copper through hydrogen reducing again under hot conditions, thereby can use repeatedly.So just, available anaerobic air-flow through the copper post is created anaerobic environment, makes culture and aerobic environment isolated, with the acquisition anerobe necessary envrionment conditions of growing.

At present, be to adopt certain peripheral unit for general ways such as the viable bacteria cultivation of anerobe, mobility observation, bacterial count, as anaerobism incubator or anaerobism culture bag.The anaerobism incubator is that the mode by gas displacement makes its inside reach anaerobic environment, needs to prepare in addition displacement with rare gas element (as high pure nitrogen) or carbon dioxide, and equipment drops into high, complicated operation, can not reach the complete anaerobic condition in the case.The anaerobism bag is by deoxygenation reagent bag the air in the sealing bag to be carried out deoxygenation, reaches anaerobic environment in the sealing bag thereby make, but because the anaerobism bag directly is not positioned in the culture dish, therefore to the Ex-all fully of the oxygen in the culture dish, and is easy to introduce living contaminants.Studies show that, adopting traditional culture dish is anaerobic bacteria culture complex operation and the expensive major cause of required equipment, culture dish surface area commonly used is bigger, make that whole substratum and air contact area are very big, oxygen molecule can penetrate substratum easily, the existence of oxygen is suppressed the vital movement of anaerobion, even causes its death, thereby has influenced the accuracy of the viable bacteria cultivation, mobility observation, bacterial count etc. of anerobe.

Publication No. be CN2146491Y's " Disposable culture dish for anaerobe ", the contact area of itself and air is very big, and sterilization process is loaded down with trivial details, can not reach complete anaerobism, has increased the additional cost input again; Publication No. is CN102358881A's " disposable anaerobic bacteria culture flat board " complex structure, complex operation, can introduce oxygen contamination during unlatching, and can not carry out operations such as mobility observation, bacterial count; Publication No. is that CN201089771Y's " anaerobism live bacterial count flat board " port area is big, can not get rid of the residual oxygen that exists in the substratum fully, and the stopping property of opening part is poor, can not be used for the liquid culture of bacterial classification, make its use be subjected to certain limitation.

The utility model content

Technical problem to be solved in the utility model provides a kind of anaerobic species culturing bottle ware simple in structure, easy and simple to handle.

For addressing the above problem, a kind of anaerobic species culturing bottle ware described in the utility model is characterized in that: it comprises the interior plug that linked together by lid and cylinder, thin box and be provided with bottle and the dome of bottleneck is rectangle; The interface of described bottle and described bottleneck is S-type, and this bottle is connected with bottleneck by described bottleneck; Be provided with described interior plug in the described bottleneck, be provided with described dome in this beyond the Great Wall; Be provided with a big envelope that matches in described beyond the Great Wall, this big envelope links to each other with the inwall circle centre position of described dome.

The internal diameter of described big envelope all equates with the lid footpath of described interior plug and the external diameter of described bottleneck.

The cylinder external diameter of plug equates with the internal diameter of described bottleneck in described.

The length of described bottleneck is less than the length of plug in described.

The edge of described big envelope from the tie point of itself and described dome to described big envelope do not have indentation.

The utility model compared with prior art has the following advantages:

1, since in the utility model the length of bottleneck therefore, can make bottle mouth position each several part closure fully less than the length of interior plug, reach the purpose of complete anaerobism.

2, since in the utility model the edge of big envelope from the tie point of itself and described dome to big envelope do not have indentation, therefore, when dome was started, big envelope is unlikely to be opened simultaneously, the sealing that is conducive to whole bottle is complete, fully reaches the condition of anaerobism.

3, owing to the interface of bottle in the utility model and bottleneck is S-type, therefore, is convenient to place microscopically to observe in the bottle ware, and can reaches the purpose of complete anaerobism.

4, simple in structure, easy and simple to handle, the conserve space of the utility model can directly place microscopically to observe and use, and can be widely used in cultivation and the observation of aerobic, anaerobism or amphimicrobe.

Description of drawings

Below in conjunction with accompanying drawing embodiment of the present utility model is described in further detail.

Fig. 1 is front view of the present utility model.

Fig. 2 is side-view of the present utility model.

Among the figure: 1-big envelope 2-interior plug 3-bottleneck 4-bottleneck 5-bottle 6-dome.

Embodiment

As shown in Figure 1 and Figure 2, a kind of anaerobic species culturing bottle ware, it comprises the interior plug 2 that linked together by lid and cylinder, thin box and be provided with bottle 5 and the dome 6 of bottleneck 4 is rectangle.Bottle 5 is S-type with the interface of bottleneck 4, and this bottle 5 is connected with bottleneck 3 by bottleneck 4; Plug 2 in being provided with in the bottleneck 3, plug 2 is provided with dome 6 in this; Interior plug 2 is provided with a big envelope that matches 1, and this big envelope 1 links to each other with the inwall circle centre position of dome 6.

Wherein: the internal diameter of big envelope 1 all equates with the lid footpath of interior plug 2 and the external diameter of bottleneck 3.

The cylinder external diameter of interior plug 2 equates with the internal diameter of bottleneck 3.

The length of bottleneck 4 is less than the length of interior plug 2.

The edge of big envelope 1 from the tie point of itself and dome 6 to big envelope 1 do not have indentation.

Claims (5)

1. An anaerobic culture bottle dish, characterized in that: it includes an inner plug (2) connected together by a cover body and a cylinder, a rectangular thin box-shaped bottle body ( 5) and a round cap (6); the interface between the bottle body (5) and the bottleneck (4) is S-shaped, and the bottle body (5) is connected to the bottle mouth (3) through the bottleneck (4) ); said bottle mouth (3) is provided with said inner plug (2), said inner plug (2) is provided with said round cap (6); said inner plug (2) is provided with a matching An envelope (1), the envelope (1) is connected to the center of the inner wall of the round cover (6). 2. An anaerobic culture bottle dish according to claim 1, characterized in that: the inner diameter of the envelope (1) and the cap diameter of the inner plug (2) and the bottle mouth (3) The outer diameters are equal. 3 . The anaerobic culture bottle dish according to claim 1 , characterized in that: the outer diameter of the cylinder of the inner plug ( 2 ) is equal to the inner diameter of the bottle mouth ( 3 ). 4 . 4 . The anaerobic culture bottle dish according to claim 1 , characterized in that: the length of the bottleneck ( 4 ) is smaller than the length of the inner plug ( 2 ). 5. An anaerobic culture bottle dish according to claim 1, characterized in that: the envelope (1) is from the connection point with the round cover (6) to the end of the envelope (1). No nicks on the edges.

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