CN104031830A - Anaerobic bacterium culturing bottle - Google Patents

Abstract

The invention relates to an anaerobic bacterium culturing bottle. The anaerobic bacterium culturing bottle comprises a rectangular thin box-shaped bottle body provided with a bottleneck, and an internal plug and a round cover which are connected by a cover body and a cylinder body. The interface of the bottle body and the bottleneck has an S shape, and the bottle is connected with a bottle mouth through the bottleneck; the internal plug is arranged in the bottle mouth, and the internal plug is provided with the round cover; and the internal plug is provided with a matching envelope, and the envelope is connected with the circle center of the inner wall of the round cover. The bottle has the advantages of simple structure, simple operation, space saving, and realization of the direct observation under a microscope, and can be widely used in the culturing and the observation of aerobic, anaerobic or facultative anaerobic microbes.

Description

A kind of culture bottle dish of anaerobic bacteria

technical field

The invention relates to a culture bottle dish, in particular to a culture bottle dish for anaerobic bacteria.

Background technique

In strict anaerobic microbial cells, there is no superoxide dismutase activity and catalase activity, and these two enzymes are directly related to the metabolism of oxygen and oxygen derivatives in the cell, so there is a very small amount in the environment Oxygen can inhibit the life activities of anaerobic microorganisms, and even cause their death. Hengate anaerobic device - copper column oxygen removal system, the principle is: the gas in the cylinder (such as nitrogen, carbon dioxide, etc.) contains a trace of oxygen, when the gas from the cylinder passes through the copper column with a temperature of about 350 ° C, the copper and The oxygen in the gas reacts and oxidizes into copper oxide, so as to achieve the purpose of purifying the oxygen in the gas; the copper pillar turns black after oxidation, and can be reduced to copper by hydrogen under high temperature conditions, so that it can be used repeatedly. In this way, the anaerobic environment can be created by the oxygen-free flow through the copper column, and the culture is isolated from the aerobic environment to obtain the necessary environmental conditions for the growth of anaerobic bacteria.

At present, the general method for live culture, motility observation, and bacterial counting of anaerobic bacteria is to use certain peripheral devices, such as anaerobic incubators or anaerobic culture bags. The anaerobic incubator achieves an anaerobic environment through gas replacement. It is necessary to prepare an inert gas (such as high-purity nitrogen) or carbon dioxide gas for replacement. The equipment investment is high and the operation is complicated, so it cannot achieve complete anaerobic environment in the box. condition. The anaerobic bag is to deoxygenate the air in the sealed bag through the deoxygenation reagent bag, so that the sealed bag can reach an anaerobic environment, but because the anaerobic bag is not directly placed in the petri dish, it cannot affect the oxygen in the petri dish. Completely clean, and easy to introduce bacteria pollution. Studies have shown that the use of traditional culture dishes is the main reason for the cumbersome operation of anaerobic bacteria cultivation and the expensive equipment required. The surface area of commonly used culture dishes is large, so that the entire culture medium has a large contact area with air, and oxygen molecules can easily penetrate In the culture medium, the existence of oxygen inhibits the life activities of anaerobic microorganisms, and even causes them to die, thus affecting the accuracy of live culture, motility observation, and bacterial counting of anaerobic bacteria.

The "disposable anaerobic culture dish" with the publication number CN2146491Y has a large contact area with the air, and the sterilization process is cumbersome, which can neither achieve complete anaerobic, but also increases additional cost input; the "disposable anaerobic culture dish" with the publication number CN102358881A The "disposable anaerobic bacteria culture plate" has a complex structure and cumbersome operation, and oxygen pollution will be introduced when it is opened, and operations such as motility observation and bacterial counting cannot be performed; the "anaerobic viable bacteria counting plate" with the publication number CN201089771Y has a large opening area, It is impossible to completely eliminate the residual oxygen existing in the culture medium, and the sealing of the opening is poor, so it cannot be used for the liquid culture of bacteria, so its use is subject to certain limitations.

Contents of the invention

The technical problem to be solved by the present invention is to provide a kind of anaerobic culture bottle dish with simple structure and convenient operation.

In order to solve the above problems, a kind of anaerobic culture bottle dish according to the present invention is characterized in that it includes a rectangular thin box-shaped bottle with a bottleneck, an inner bottle connected by a cover and a cylinder. A stopper and a round cap; the interface between the bottle body and the bottleneck is S-shaped, and the bottle body is connected with a bottleneck through the bottleneck; the inner plug is provided in the bottleneck, and the inner plug is provided with There is the round cover; the inner plug is provided with a matching envelope, and the envelope is connected with the center of the inner wall of the round cover.

The inner diameter of the envelope is equal to the cap diameter of the inner plug and the outer diameter of the bottle mouth.

The cylinder outer diameter of the inner plug is equal to the inner diameter of the bottle mouth.

The length of the bottleneck is less than the length of the inner plug.

The envelope is unscored from its point of attachment to the dome to the edge of the envelope.

Compared with the prior art, the present invention has the following advantages:

1. Because the length of the bottleneck is less than the length of the inner plug in the present invention, each part of the bottleneck can be completely closed to achieve the purpose of complete anaerobic.

2. Since there is no notch on the envelope from the connection point with the round cap to the edge of the envelope in the present invention, the envelope will not be opened at the same time when the round cap is lifted, which is conducive to the integrity of the entire bottle body seal , to fully meet the anaerobic conditions.

3. Because the interface between the bottle body and the bottleneck is S-shaped in the present invention, it is convenient to place the bottle dish under a microscope for observation, and can achieve the purpose of complete anaerobic.

4. The invention has the advantages of simple structure, easy operation, space saving, can be directly placed under a microscope for observation and use, and can be widely used in the cultivation and observation of aerobic, anaerobic or facultative anaerobic microorganisms.

Description of drawings

The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings.

Fig. 1 is the front view of the present invention.

Figure 2 is a side view of the present invention.

In the figure: 1—envelope 2—inner plug 3—bottle mouth 4—bottle neck 5—bottle body 6—round cap.

Detailed ways

As shown in Fig. 1 and Fig. 2, a kind of anaerobic culture bottle dish, it comprises the bottle body 5 that is rectangular thin box shape and is provided with bottleneck 4, the inner plug 2 that is connected together by lid body and cylinder and Round cover6. The interface between the bottle body 5 and the bottleneck 4 is S-shaped, and the bottle body 5 is connected with the bottleneck 3 through the bottleneck 4; the bottleneck 3 is provided with an inner plug 2, and the inner plug 2 is provided with a round cap 6; 2 is provided with a matching envelope 1, and the envelope 1 is connected to the center of the inner wall of the round cover 6.

Wherein: the inner diameter of the envelope 1 is equal to the cap diameter of the inner plug 2 and the outer diameter of the bottle mouth 3 .

The outer diameter of the cylinder of the inner plug 2 is equal to the inner diameter of the bottle mouth 3 .

The length of the bottleneck 4 is less than the length of the inner plug 2 .

The envelope 1 has no score from its connection point with the dome 6 to the edge of the envelope 1 .

Claims (5)

1. an anaerobic species culturing bottle ware, is characterized in that: it comprises and is rectangle thin box and is provided with the bottle (5) of bottleneck (4), the interior plug (2) being linked together by lid and cylinder and dome (6); Described bottle (5) is S-type with the interface of described bottleneck (4), and this bottle (5) is connected with bottleneck (3) by described bottleneck (4); In described bottleneck (3), be provided with described interior plug (2), this interior plug (2) is provided with described dome (6); Described interior plug (2) is provided with a big envelope matching (1), and this big envelope (1) is connected with the inwall circle centre position of described dome (6).

2. a kind of anaerobic species culturing bottle ware as claimed in claim 1, is characterized in that: the internal diameter of described big envelope (1) all equates with the lid footpath of described interior plug (2) and the external diameter of described bottleneck (3).

3. a kind of anaerobic species culturing bottle ware as claimed in claim 1, is characterized in that: the cylinder external diameter of described interior plug (2) equates with the internal diameter of described bottleneck (3).

4. a kind of anaerobic species culturing bottle ware as claimed in claim 1, is characterized in that: the length of described bottleneck (4) is less than the length of described interior plug (2).

5. a kind of anaerobic species culturing bottle ware as claimed in claim 1, is characterized in that: the edge of described big envelope (1) from the tie point of itself and described dome (6) to described big envelope (1) is without indentation.