CN210765329U - Low-pressure and low-oxygen cell experimental device - Google Patents
Low-pressure and low-oxygen cell experimental device Download PDFInfo
- Publication number
- CN210765329U CN210765329U CN201921203934.XU CN201921203934U CN210765329U CN 210765329 U CN210765329 U CN 210765329U CN 201921203934 U CN201921203934 U CN 201921203934U CN 210765329 U CN210765329 U CN 210765329U
- Authority
- CN
- China
- Prior art keywords
- cabinet body
- low
- cell
- conveying pipe
- oxygen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The utility model discloses a low-pressure and low-oxygen cell experimental device, which comprises a cabinet body, wherein the top of the cabinet body is provided with a sealing cover, the bottom of the cabinet body is provided with a constant-temperature mixing instrument, the upper surface of the constant-temperature mixing instrument is provided with a cell vessel, the constant-temperature mixing instrument penetrates through the bottom, and the cell vessel is positioned in the cabinet body; the cabinet body is respectively connected with a vacuumizing device and a gas conveying device through pipelines; the cabinet body is also provided with a nutrition conveying device. The utility model discloses a set up gas conveying equipment, temperature-sensing ware, humidity inductor, forced induction ware, constant temperature mixing appearance, be convenient for carry out the cell experiment under the low oxygen environment. The vacuum pumping equipment is arranged, so that the internal pressure of the cabinet body can be increased and reduced conveniently, and the nutrient solution can be increased conveniently by arranging the nutrient conveying device, so that cells on the cell vessel can grow; thereby achieving the effect of facilitating cell growth experiments under low pressure.
Description
Technical Field
The utility model belongs to the technical field of biological assay device, a low pressure hypoxia cell experimental apparatus is related to.
Background
In the prior art, animals are mostly used for carrying out required simulation experiments in pathological research under the low-pressure and low-oxygen conditions, the required devices and materials occupy larger space, the required devices are complicated and expensive, and the control precision is difficult to meet the requirement. The low-pressure hypoxia simulation experiment adopting the cell to replace the animal along with the scientific development is a new experimental method in the pathological research under the low-pressure hypoxia condition in the current medical science.
The current cell experiment device is complex in the experiment operation process, is not beneficial to the operation of experimenters and influences the experiment result.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a low pressure hypoxia cell experimental apparatus for cell experiment research under the low pressure hypoxia condition.
The technical scheme adopted by the utility model is that the low-pressure and low-oxygen cell experimental device comprises a cabinet body, wherein a sealing cover is arranged at the top of the cabinet body, a constant-temperature mixing instrument is arranged at the bottom of the cabinet body, a cell vessel is arranged on the upper surface of the constant-temperature mixing instrument, the constant-temperature mixing instrument penetrates through the bottom of the cabinet body, the constant-temperature mixing instrument is fixedly and hermetically connected with the cabinet body, and the cell vessel is positioned in the cabinet body; the cabinet body is respectively connected with a vacuumizing device and a gas conveying device through pipelines; the cabinet body is also provided with a nutrition conveying device.
The utility model is also characterized in that,
the inner wall of the cabinet body is provided with a limiting block near the bottom, and the limiting block is near the upper surface of the constant-temperature blending instrument.
The vacuumizing equipment comprises a connecting pipe, one end of the connecting pipe penetrates through the side wall of the cabinet body and is communicated with the interior of the cabinet body, and the other end of the connecting pipe is connected with a vacuumizing pump;
the gas conveying equipment comprises an oxygen conveying pipe, a nitrogen conveying pipe and a carbon dioxide conveying pipe, one end of the oxygen conveying pipe, the nitrogen conveying pipe and one end of the carbon dioxide conveying pipe penetrate through the side wall of the cabinet body and are communicated with the inside of the cabinet body, and the other ends of the oxygen conveying pipe, the nitrogen conveying pipe and the carbon dioxide conveying pipe are connected with corresponding gas storage equipment.
Flow control valves are respectively arranged on the oxygen conveying pipe, the nitrogen conveying pipe and the carbon dioxide conveying pipe.
The top of the sealing cover is provided with a handle.
The surface of the cabinet body is provided with a display, a temperature sensor, a humidity sensor and a pressure sensor are arranged in the cabinet body, and the temperature sensor, the humidity sensor and the pressure sensor are respectively electrically connected with the display.
The nutrition conveying device comprises an ejector, the ejection end of the ejector penetrates through the side wall of the cabinet body and extends into the cabinet body, and the other end of the ejector is connected with the nutrition liquid bottle.
The cabinet body is also provided with an observation device.
The observation device comprises a microscope, the microscope is arranged at the top of the expansion bracket, the bottom of the expansion bracket is fixedly connected with the flat plate, the side surface of the flat plate is fixedly connected with the bottom of the side wall of the cabinet body 1, and the observation end of the microscope extends into the cabinet body.
The side wall of the cabinet body is provided with a PC board, the PC board is provided with a through hole which is matched with the microscope observation end, and the microscope observation end extends into the cabinet body through the through hole.
The utility model has the advantages that,
1. the utility model is provided with the cabinet body, the sealing cover and the handle, so that the cabinet body can be conveniently opened to place a cell vessel; the oxygen conveying pipe, the nitrogen conveying pipe and the carbon dioxide conveying pipe are arranged, so that the survival condition of cells under various gas contents is facilitated; the cell growth environment is conveniently detected by arranging the temperature sensor, the humidity sensor and the pressure sensor; through setting up the constant temperature blending appearance, be convenient for control cabinet body inside temperature to reach the effect of being convenient for carry out the cell experiment under the low oxygen environment.
2. The utility model is provided with the connecting pipe and the vacuum pump, thereby facilitating the pressurization and decompression inside the cabinet body; by arranging the ejector and the nutrient solution bottle, the nutrient solution is increased conveniently to grow the cells on the cell vessel; through setting up flat board, expansion bracket and microscope, be convenient for observe the cell growth situation of the internal portion of cabinet to reach the effect of the cell growth experiment of being convenient for under the low pressure.
Drawings
FIG. 1 is a schematic diagram of the low-pressure hypoxic cell experimental device according to the present invention;
fig. 2 is a schematic side view of the low-pressure hypoxic cell experimental device of the present invention.
In the figure, 1, a cabinet body, 2, a thread groove, 3, a sealing cover, 4, a handle, 5, an oxygen conveying pipe, 6, a nitrogen conveying pipe, 7, a carbon dioxide conveying pipe, 8, a temperature sensor, 9, a humidity sensor, 10, a pressure sensor, 11, a constant-temperature blending instrument, 12, a limiting block, 13, a cell container, 14, a connecting pipe, 15, a vacuum pumping pump, 16, an ejector, 17, a nutrient solution bottle, 18, a flat plate, 19, an expansion bracket and 20, a microscope.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
The utility model relates to a structure of low pressure hypoxia cell experimental apparatus, as shown in fig. 1, 2, including the cabinet body 1, 1 top of the cabinet body is provided with sealed lid 3, and 1 internal surface of the cabinet body is close to the top and is provided with thread groove 2, and 3 surfaces of sealed lid are provided with the screw thread corresponding with thread groove 2, and sealed lid 3 and the cabinet body 1 is through screw-thread fit sealing connection. The bottom of the cabinet body 1 is provided with a constant-temperature blending instrument 11, the upper surface of the constant-temperature blending instrument 11 is provided with a cell vessel 13, the constant-temperature blending instrument 11 penetrates through the bottom of the cabinet body 1, the constant-temperature blending instrument 11 is fixedly and hermetically connected with the cabinet body 1, and the cell vessel 13 is positioned in the cabinet body 1; the cabinet body 1 is respectively connected with a vacuumizing device and a gas conveying device through pipelines; the cabinet body 1 is also provided with a nutrition conveying device.
The inner wall of the cabinet body 1 is provided with a limiting block 12 near the bottom, and the limiting block 12 is near the upper surface of the constant temperature blending instrument 11. The limiting block 12 is used for limiting the placing position of the cell vessel 13 and has a stabilizing effect.
The vacuumizing equipment comprises a connecting pipe 14, one end of the connecting pipe 14 penetrates through the side wall of the cabinet body 1 and is communicated with the inside of the cabinet body 1, and the other end of the connecting pipe 14 is connected with a vacuumizing pump 15; through setting up evacuation equipment, be convenient for carry out pressure boost and decompression in the internal cell experiment process of cabinet.
The gas delivery equipment includes oxygen conveyer pipe 5, nitrogen gas conveyer pipe 6 and carbon dioxide conveyer pipe 7, and 1 lateral wall of the cabinet body is passed to oxygen conveyer pipe 5, nitrogen gas conveyer pipe 6 and carbon dioxide conveyer pipe 7 one end, and with the inside intercommunication of the cabinet body 1, the oxygen conveyer pipe 5, the nitrogen gas conveyer pipe 6 and the carbon dioxide conveyer pipe 7 other end are connected with corresponding gaseous storage facilities.
The oxygen delivery pipe 5, the nitrogen delivery pipe 6 and the carbon dioxide delivery pipe 7 are respectively provided with a flow control valve. Through setting up gas conveying equipment, be convenient for detect the survival situation of cell under various gas content.
The top of the sealing cover 3 is provided with a handle 4.
The surface of the cabinet body 1 is provided with a display, a temperature sensor 8, a humidity sensor 9 and a pressure sensor 10 are arranged in the cabinet body 1, and the temperature sensor 8, the humidity sensor 9 and the pressure sensor 10 are respectively electrically connected with the display. The temperature sensor 8, the humidity sensor 9 and the pressure sensor 10 are arranged, so that the growth environment condition of the cells can be conveniently detected.
The nutrition conveying device comprises an ejector 16, the spraying end of the ejector 16 penetrates through the side wall of the cabinet body 1 and extends into the cabinet body 1, and the other end of the ejector 16 is connected with a nutrition liquid bottle 17. The nutrient delivery device may be a push-type spray device such as a conventional watering can used for watering flowers, or a squeeze-type spray device such as laboratory wash bottles. Through setting up nutrition conveyor, be convenient for increase the inside nutrient solution of cabinet body and make carrying out cell growth in the cell household utensils.
The cabinet body 1 is also provided with an observation device. The observation device comprises a microscope 20, the microscope 20 is arranged at the top of the telescopic frame 19, the bottom of the telescopic frame 19 is fixedly connected with the flat plate 18, the side surface of the flat plate 18 is fixedly connected with the bottom of the side wall of the cabinet body 1, and the observation end of the microscope 20 extends into the cabinet body 1.
The side wall of the cabinet body 1 is provided with a PC board, the PC board is provided with a through hole which is matched with the observation end of the microscope 20, and the observation end of the microscope 20 extends into the cabinet body 1 through the through hole. Through setting up viewing device, be convenient for observe the cell growth situation of cabinet internal portion.
During the use, through the sealed lid 3 of handle 4 rotatory drive open cabinet body 1, and put the constant temperature that is equipped with the cell protomer 13 on the diapire in the cabinet body 1 and mix on the appearance 11, and lock sealed lid 3, simultaneously with close oxygen conveyer pipe 5, nitrogen gas conveyer pipe 6 and carbon dioxide conveyer pipe 7, extract the inside air of cabinet body 1 through evacuation pump 15, and observe the condition of the inside cell growth division of cabinet body 1 through microscope 20, and suitable increase nutrient solution and oxygen and pressure, thereby reach and be convenient for carry out the cell growth experiment under the low oxygen low pressure environment.
Claims (10)
1. The low-pressure and low-oxygen cell experimental device is characterized by comprising a cabinet body (1), wherein a sealing cover (3) is arranged at the top of the cabinet body (1), a constant-temperature blending instrument (11) is arranged at the bottom of the cabinet body (1), a cell vessel (13) is arranged on the upper surface of the constant-temperature blending instrument (11), the constant-temperature blending instrument (11) penetrates through the bottom of the cabinet body (1), the constant-temperature blending instrument (11) is in sealing connection with the cabinet body (1), and the cell vessel (13) is positioned in the cabinet body (1); the cabinet body (1) is respectively connected with a vacuumizing device and a gas conveying device through pipelines; the cabinet body (1) is also provided with a nutrition conveying device.
2. The low-pressure and low-oxygen cell experimental device as claimed in claim 1, wherein the inner wall of the cabinet body (1) is provided with a limiting block (12) near the bottom, and the limiting block (12) is near the upper surface of the constant-temperature blending instrument (11).
3. The low-pressure hypoxic cell experimental device according to claim 1, wherein the vacuum-pumping equipment comprises a connecting pipe (14), one end of the connecting pipe (14) penetrates through the side wall of the cabinet body (1) and is communicated with the inside of the cabinet body (1), and the other end of the connecting pipe (14) is connected with a vacuum-pumping pump (15);
the gas conveying equipment comprises an oxygen conveying pipe (5), a nitrogen conveying pipe (6) and a carbon dioxide conveying pipe (7), one end of the oxygen conveying pipe (5), the nitrogen conveying pipe (6) and one end of the carbon dioxide conveying pipe (7) penetrate through the side wall of the cabinet body (1), the oxygen conveying pipe (5), the nitrogen conveying pipe (6) and the carbon dioxide conveying pipe (7) are communicated with the inside of the cabinet body (1), and the other ends of the oxygen conveying pipe (5), the nitrogen conveying pipe (6) and the carbon dioxide conveying pipe.
4. An hypoxic cell assay device according to claim 3, wherein the oxygen (5), nitrogen (6) and carbon dioxide (7) ducts are provided with flow control valves, respectively.
5. An hypoxic cell assay device according to claim 1, wherein the sealing lid (3) is provided with a handle (4) at the top.
6. The low-pressure and low-oxygen cell experiment device as claimed in claim 1, wherein a display is arranged on the surface of the cabinet body (1), a temperature sensor (8), a humidity sensor (9) and a pressure sensor (10) are arranged in the cabinet body (1), and the temperature sensor (8), the humidity sensor (9) and the pressure sensor (10) are respectively electrically connected with the display.
7. The hypoxic cell experimental apparatus according to claim 1, wherein the nutrition delivery device comprises an injector (16), the injection end of the injector (16) penetrates through the side wall of the cabinet (1) and extends into the cabinet (1), and the other end of the injector (16) is connected with a nutrition liquid bottle (17).
8. An hypoxic cell assay device according to any one of claims 1 to 7, wherein the cabinet (1) is further provided with an observation device.
9. The low-pressure and low-oxygen cell experiment device as claimed in claim 8, wherein the observation device comprises a microscope (20), the microscope (20) is arranged at the top of the telescopic frame (19), the bottom of the telescopic frame (19) is fixedly connected with the flat plate (18), the side surface of the flat plate (18) is fixedly connected with the bottom of the side wall of the cabinet body (1), and the observation end of the microscope (20) extends into the cabinet body (1).
10. The experimental apparatus for low-pressure and low-oxygen cell as claimed in claim 9, wherein the cabinet (1) has a PC board on the sidewall, the PC board has a through hole corresponding to the observation end of the microscope (20), and the observation end of the microscope (20) extends into the cabinet (1) through the through hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921203934.XU CN210765329U (en) | 2019-07-29 | 2019-07-29 | Low-pressure and low-oxygen cell experimental device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921203934.XU CN210765329U (en) | 2019-07-29 | 2019-07-29 | Low-pressure and low-oxygen cell experimental device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210765329U true CN210765329U (en) | 2020-06-16 |
Family
ID=71033408
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921203934.XU Active CN210765329U (en) | 2019-07-29 | 2019-07-29 | Low-pressure and low-oxygen cell experimental device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210765329U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110452819A (en) * | 2019-07-29 | 2019-11-15 | 西安医学院 | A kind of observable hypobaric hypoxia cell incubator |
| CN115948243A (en) * | 2022-09-30 | 2023-04-11 | 河北医科大学第二医院 | Special low oxygen workstation of high connotation |
| CN116769595A (en) * | 2023-06-19 | 2023-09-19 | 中国人民解放军空军军医大学 | Low-pressure low-oxygen cell simulator |
-
2019
- 2019-07-29 CN CN201921203934.XU patent/CN210765329U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110452819A (en) * | 2019-07-29 | 2019-11-15 | 西安医学院 | A kind of observable hypobaric hypoxia cell incubator |
| CN115948243A (en) * | 2022-09-30 | 2023-04-11 | 河北医科大学第二医院 | Special low oxygen workstation of high connotation |
| CN116769595A (en) * | 2023-06-19 | 2023-09-19 | 中国人民解放军空军军医大学 | Low-pressure low-oxygen cell simulator |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN210765329U (en) | Low-pressure and low-oxygen cell experimental device | |
| CN104565808B (en) | Rigid container gas displacement method and device based on quantitative pumping inflation technology | |
| EP2316314A3 (en) | Breastmilk handling apparatus particularly useful for warming of breastmilk containers such as bottles and syringes | |
| CN110452819A (en) | A kind of observable hypobaric hypoxia cell incubator | |
| CN203128565U (en) | Anaerobic bacterium culture bottle vessel | |
| CN202735127U (en) | Sample storage tube | |
| CN202477459U (en) | Pickled vegetable container | |
| CN207767090U (en) | A kind of agricultural edible fungi inoculation device | |
| US5219755A (en) | Process for the culture of anaerobics germs | |
| CN212335168U (en) | In-vitro fermentation system | |
| CN204151250U (en) | A kind of feed supplement tank with steam sterilizer | |
| CN214916063U (en) | Sectional sampling reaction kettle | |
| CN211770325U (en) | Portable device of carbon dioxide generator | |
| CN216738288U (en) | Microbial culture device for medical inspection | |
| CN209576659U (en) | A kind of drug synthetic reaction kettle device | |
| CN203960185U (en) | A kind of anaerobic fermentation system | |
| CN209685627U (en) | A kind of novel household food waste composting device | |
| CN209291011U (en) | A kind of Lumbrokinase enzyme preparation storage device | |
| CN209609666U (en) | A kind of pressure-adjusting type biological sample device for casting | |
| CN218290960U (en) | Cell culture dish with adjustable environmental gas | |
| CN202362179U (en) | Fumigation device for soil microorganisms | |
| CN220132229U (en) | Microgravity cell culture dish | |
| CN104031830A (en) | Anaerobic bacterium culturing bottle | |
| CN214830385U (en) | Mixed starter culture bacterial of lactic acid bacteria saccharomycete keeps equipment | |
| CN202322311U (en) | Medical liquid medicine split charging tank |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |