CN115948243A - Special low oxygen workstation of high connotation - Google Patents

Special low oxygen workstation of high connotation Download PDF

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Publication number
CN115948243A
CN115948243A CN202211209091.0A CN202211209091A CN115948243A CN 115948243 A CN115948243 A CN 115948243A CN 202211209091 A CN202211209091 A CN 202211209091A CN 115948243 A CN115948243 A CN 115948243A
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CN
China
Prior art keywords
sealing
side wall
fixed
observation
assembly
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Pending
Application number
CN202211209091.0A
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Chinese (zh)
Inventor
张祥建
陈荣
张笑
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Second Hospital of Hebei Medical University
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Second Hospital of Hebei Medical University
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Priority to CN202211209091.0A priority Critical patent/CN115948243A/en
Publication of CN115948243A publication Critical patent/CN115948243A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a special low-oxygen workstation for high connotation, which comprises: the observation device comprises a high content cell analyzer and a sealing component for maintaining a low oxygen environment for the high content cell analyzer; the observation assemblies are respectively arranged on the top and the side wall of the sealing assembly; and a supply device for providing a low-oxygen environment for the observation device and maintaining a low-temperature dry environment inside the observation device. The invention has the beneficial effects that: the high content workstation for the hypoxia environment is convenient to control, and cells can be observed in the hypoxia environment; a condensing system is arranged to prevent the activity of the cells from being influenced by overhigh internal temperature; and a drying system is arranged to discharge excessive water inside, so that a dry cell observation environment is provided.

Description

Special hypoxemia workstation of high content
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a high-content special low-oxygen workstation.
Background
The high-content special hypoxia workstation is used for simulating a physiological hypoxia state in a living body, so that the in vitro research result is closer to the real level in the body; or to simulate the pathological state of an organism in the presence of specific hypoxia, in order to study the relevant mechanisms and hypoxic diseases.
Disclosure of Invention
In order to overcome the problems in the background art, the invention provides a high-content special low-oxygen workstation.
A high content specialized low oxygen workstation comprising:
the observation device comprises a high content cell analyzer and a sealing component for maintaining a low oxygen environment for the high content cell analyzer;
the observation assemblies are respectively arranged on the top and the side wall of the sealing assembly;
and a supply device for providing a low-oxygen environment for the observation device and maintaining a low-temperature dry environment inside the observation device.
Further, the seal assembly includes a top seal structure, a sidewall seal structure, and a bottom seal structure; the bottom sealing structure is separated from the side wall sealing structure and is fixed at the bottom of the side wall sealing structure; the top sealing structure and the side wall sealing structure are separated and the top of the side wall sealing structure is fixed;
further, the observation assembly comprises a first observation position, a second observation position and a third observation position; the first observation position and the second observation position are both arranged at the top of the sealing assembly; the third observation position is arranged on the side wall of the sealing component; the first observation position is separated from the sealing assembly and is fixed at the top of the sealing assembly; the second observation position is separated from the sealing assembly and is fixed at the top of the sealing assembly; the third observation position is separated from the sealing assembly and is fixed on the side wall of the sealing assembly.
Further, the first observation position is separated from the top sealing structure and is fixed on the top of the top sealing structure; the second observation position is separated from the top sealing structure and is fixed at the top of the top sealing structure; the third observation position is separated from the side wall sealing structure and is fixed on the side wall of the side wall sealing structure.
Further, a pipeline port is formed in the side wall of the sealing assembly; the pipeline port and the sealing assembly are separated and fixed on the side wall of the sealing assembly; the pipeline port and the side wall sealing structure are separated and fixed on the side wall of the side wall sealing structure.
Further, the supply device comprises a control structure, a low oxygen supply assembly and an auxiliary assembly; the control structure is connected with the low oxygen supply assembly and the auxiliary assembly through leads.
Further, the hypoxia supply component comprises a gas cylinder, a pressure gauge, a gas valve and a gas guide tube; the pressure gauge and the air valve are both arranged at the top of the air bottle; one end of the air valve, which is far away from the air bottle, is connected with the air guide pipe; one end of the air duct far away from the air valve is connected with the sealing component; the gas cylinder is a nitrogen cylinder.
Further, the auxiliary assembly comprises an outer circulation machine, an inner circulation machine and a circulation conduit; the circulating outer machine is connected with the circulating inner machine through a circulating conduit; the circulating inner machine is arranged inside the sealing assembly; the outdoor circulation unit is disposed outside the sealing assembly.
Further, the circulation indoor unit comprises a first shell, a first exchanger and a fan; the first exchanger and the fan are both arranged inside the first shell; the fan is opposite to the first exchanger; the first exchanger is connected with an external circulation machine through a circulation conduit; the first exchanger is separated from the first shell and is fixed on the inner wall of the first shell; the fan is separated from the first casing and fixed on the inner wall of the first casing.
Further, the outdoor circulating machine comprises a second shell, a second exchanger, a heat dissipation fan and a compressor; the second exchanger, the heat dissipation fan and the compressor are arranged in the second shell; the heat dissipation fan is opposite to the second exchanger; the second exchanger is connected with the circulation inner machine through a circulation conduit; the second exchanger is separated from the second shell and is fixed on the inner wall of the second shell; the heat dissipation fan is separated from the second shell and is fixed on the inner wall of the second shell; the compressor and the second shell are separated and fixed on the inner wall of the second shell.
The invention has the beneficial effects that: the high content workstation for the hypoxia environment is convenient to control, and cells can be observed in the hypoxia environment; a condensing system is arranged to prevent the activity of the cells from being influenced by overhigh internal temperature; and a drying system is arranged to discharge excessive water inside, so that a dry cell observation environment is provided.
Drawings
FIG. 1 is a schematic diagram of the structure of a product embodying the present invention;
FIG. 2 is a front view of a product embodying the present invention;
FIG. 3 is a schematic view of a viewing device embodying the present invention;
FIG. 4 is a cross-sectional view of a viewing device embodying the present invention;
FIG. 5 is a schematic structural view of a locking structure embodying the present invention;
FIG. 6 is a cross-sectional view of a locking structure embodying the present invention;
FIG. 7 is a schematic view of a feed apparatus embodying the present invention;
in the figure, 1, observation apparatus; 11. a top seal structure; 111. a first mounting bracket; 112. a first viewing plate; 113. a first observation position; 114. a second observation position; 12. a side wall sealing structure; 121. a second mounting bracket; 122. a second viewing plate; 123. a third observation position; 124. fixing a bracket; 125. a pipeline port; 13. a bottom sealing structure; 14. a locking structure; 141. a fixing plate; 142. a first telescopic arm; 143. a second telescopic arm; 144. a bayonet lock; 145. a control member; 146. a fixed mount; 15. a high content cell analyzer; 151. a power line; 2. a supply device; 21. a control structure; 22. a low oxygen supply assembly; 221. a nitrogen gas cylinder; 222. a pressure gauge; 223. an air valve; 224. a gas-guide tube; 23. an auxiliary component; 231. an external circulation unit; 232. circulating the inner machine; 233. a circulation conduit.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below by specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and other advantages and effects of the present invention can be easily understood by those skilled in the art from the disclosure of the present specification. The present invention can be implemented or applied by other different specific embodiments, and the features in the following embodiments and embodiments can be combined with each other without conflict, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
A high content specialized hypoxic workstation as shown in fig. 1-7, comprising:
an observation device 1 including a high content cell analyzer 15 and a sealing member for maintaining a hypoxic environment for the high content cell analyzer 15;
the observation assemblies are respectively arranged on the top and the side wall of the sealing assembly;
the supply device 2 provides the low-oxygen environment to the observation device 1 and maintains a low-temperature dry environment inside the observation device 1.
Including seal assembly wrapped high content cell analyzer 15, feeding device 2 reduced the inside oxygen content of seal assembly and reduced the inside temperature and humidity of seal assembly. The physiological hypoxia state in the organism is simulated, so that the in vitro research result is closer to the real level in the organism; or to simulate the pathological state of an organism in the presence of specific hypoxia, in order to study the relevant mechanisms and hypoxic diseases.
The sealing assembly comprises a top sealing structure 11, a side wall sealing structure 12 and a bottom sealing structure 13; the top of the side wall sealing structure 12 is detachably connected with the top sealing structure 11, and the bottom of the side wall sealing structure 12 is detachably connected with the bottom sealing structure 13; the bottom sealing structure 13 is connected with the side wall seal through bolts; the top seal 11 is connected to the side wall seal 12 by a locking structure 14. Specifically, a threaded hole is formed in the side edge of the bottom sealing structure 13, and a bolt penetrates through the bottom of the side wall sealing structure 12 and then is locked with the threaded hole. Or, a plurality of through holes are formed in the side edge of the bottom sealing structure 13, a nut is arranged at the bottom of the bottom sealing structure 13, and the bolt sequentially penetrates through the bottom of the side wall sealing structure 12 and the bottom sealing structure 13 and then is locked with the nut.
As shown in fig. 3, the top sealing structure 11 includes a first mounting bracket 111 and a first viewing plate 112; the first mounting frame 111 is of a plate-shaped structure shaped like a Chinese character ri; the first observation plate 112 is installed at the hollow-out position of the first installation frame 111, and the first observation plate 112 is fixedly connected with the first installation frame 111. The locking structure 14 comprises a buckle. The sidewall sealing structure 12 includes a second mounting bracket 121, a second viewing plate 122, a fixed bracket 124, and a line port 125. The second mounting frame 121 has a square tubular structure, and one side facing the operator is hollowed out. The second observation plate 122 is disposed at the hollow portion of the second mounting frame 121, and the second observation plate 122 is fixedly connected to the second mounting frame 121. The second mounting frame 121 is connected with the bottom sealing structure 13 through bolts, and a mounting plate parallel to the bottom sealing structure 13 is arranged at the bottom of the second mounting frame 121.
In some embodiments of the present application, to enhance the air-tightness of the seal assembly, a first seal ring is disposed between the first mounting bracket 111 and the second mounting bracket 121, and a second seal ring is disposed between the second mounting bracket 121 and the bottom seal structure 13. Specifically, the first sealing ring and the second sealing ring are made of rubber materials, a groove for mounting the first sealing ring is formed in the top of the second mounting frame 121, and a groove for mounting the second sealing ring is formed in the bottom of the first observation plate 112. The first sealing ring and the second sealing ring are both fixedly connected with the second mounting frame 121. Or a groove for mounting a second sealing ring is arranged at the top of the bottom sealing structure 13, and the bottom sealing structure 13 is fixedly connected with the second sealing ring; a groove for mounting a first sealing ring is formed in the top of the second mounting frame 121, and the first sealing ring is fixedly connected with the second mounting frame 121. Optionally, a groove for installing a first sealing ring is arranged at the bottom of the top sealing structure 11, and the top sealing structure 11 is fixedly connected with the first sealing ring; a groove for mounting a second sealing ring is formed at the bottom of the second mounting frame 121, and the second sealing ring is fixedly connected with the second mounting frame 121.
As shown in fig. 3, the observation component includes a first observation site 113, a second observation site 114 and a third observation site 123; the first observation position 113 and the second observation position 114 are both arranged on the top of the first observation plate 112; the third observation position 123 is arranged on a side wall of the second mounting frame 121; the first observation position 113 and the first observation plate 112 are locked by a bolt; the second observation position 114 is locked with the first observation plate 112 through a bolt; the third observation position 123 is fastened to the second mounting bracket 121 by bolts.
As shown in fig. 3, one end of the first observation position 113 is connected to the first observation plate 112 by a bolt, and the other end is connected to the first mounting bracket 111 by a hinge. Preferably, a third sealing ring is arranged between the first observation position 113 and the first observation plate 112; the third sealing ring is fixedly connected with the first observation plate 112, or the third sealing ring is fixedly connected with the first observation position 113.
As shown in fig. 3, the second observation position 114 has one end connected to the first observation plate 112 by a bolt and the other end connected to the first observation plate 112 by a hinge. Preferably, a fourth gasket is disposed between the second viewing position 114 and the first viewing plate 112; the fourth sealing ring is fixedly connected to the first viewing plate 112, or the fourth sealing ring is fixedly connected to the second viewing position 114.
As shown in fig. 3, the third observation site 123 is connected to the second observation plate 122 by bolts. Preferably, a fifth sealing ring is arranged between the third observation position 123 and the second observation plate 122; the fifth sealing ring is fixedly connected with the second observation plate 122, or the fifth sealing ring is fixedly connected with the third observation position 123. The third viewing position 123 is provided with a handle on the side facing the user for facilitating the operator to detach the third viewing position 123 from the second viewing plate 122 or to attach the third viewing position 123 to the second viewing plate 122.
As shown in fig. 3, the fixing bracket 124 and the line port 125 are both provided on a side wall of the second mounting bracket 121; the fixed bracket 124 is bolted to the second mounting bracket 121, and the second mounting bracket 121 mounts the circulator 232 inside the seal assembly. The line port 125 is fixedly connected to the second mounting bracket 121. The supply device 2 is connected to the sealing assembly via a line port 125; at the same time, the power supply line 151 of the high content cellular analyzer 15 extends out of the seal assembly through the line port 125.
As shown in fig. 5 and 6, the locking structure 14 includes a fixing plate 141, a first telescopic arm 142, a second telescopic arm 143, a detent 144, a control member 145 and a fixing frame 146; the first telescopic arm 142 and the second telescopic arm 143 are respectively disposed at both sides of a latch 144, and the latch 144 is latched with the fixing plate 141. The ends of the first telescopic arm 142 and the second telescopic arm 143 far away from the bayonet 144 are rotatably connected with a control member 145, and the control member 145 is rotatably connected with a fixed frame 146. As shown in fig. 3, the fixing plate 141 is fixedly connected to the first mounting frame 111, and the fixing frame 146 is fixedly connected to the second mounting frame 121.
The first telescopic arm 142 and the second telescopic arm 143 are both split. Specifically, the first telescopic arm 142 includes a first connecting rod, a second connecting rod and a spring, the first connecting rod and the second connecting rod are arranged in a crossed manner, and the spring is installed at the crossed portion. The first and second connecting rods are pulled by the structural characteristics of the spring, and the length of the first telescopic arm 142 is contracted, so that the bayonet 144 can be firmly clamped on the fixing plate 141. The second telescopic arm 143 has the same structure and the same working principle as the first telescopic arm 142.
As shown in fig. 2 and 7, the supply device 2 comprises a control structure 21, a low oxygen supply module 22 and an auxiliary module 23; the control structure 21 is connected to the low oxygen supply module 22 and the auxiliary module 23 by wires. The low oxygen supply assembly 22 is used to provide a low oxygen environment for the seal assembly and the auxiliary assembly 23 is used to maintain a low temperature dry environment inside the seal assembly.
The low oxygen supply assembly 22 comprises a nitrogen bottle 221, a pressure gauge 222, an air valve 223 and an air duct 224; the pressure gauge 222 and the gas valve 223 are arranged at the top of the nitrogen cylinder 221; one end of the air valve 223 far away from the nitrogen cylinder 221 is connected with the air duct 224; the end of the air duct 224 remote from the air valve 223 is connected to the sealing assembly. The control structure 21 opens the air valve 223 to fill the nitrogen gas in the nitrogen gas bottle 221 into the sealing assembly through the air duct 224, so as to provide a low oxygen condition for the high content cell analyzer 15. And the control structure 21 provides a sensor to sense the oxygen content in the sealing assembly, and adjusts the opening degree of the air valve 223 in real time, so that the oxygen content in the sealing assembly is kept stable. The pressure gauge 222 is used to monitor the pressure inside the nitrogen gas cylinder 221.
The auxiliary assembly 23 comprises an outer circulation machine 231, an inner circulation machine 232 and a circulation conduit 233; the circulation outer machine 231 is connected with the circulation inner machine 232 through a circulation conduit 233; the circulation indoor unit 232 is disposed inside the sealing assembly; an outdoor circulator 231 is disposed outside the sealing assembly.
The circulation indoor unit 232 includes a first shell, a first exchanger and a fan; the outdoor unit 231 includes a second casing, a second exchanger, a heat dissipation fan, and a compressor.
The first exchanger and the fan are both arranged inside the first shell; the fan is opposite to the first exchanger; the first exchanger and the second exchanger and the compressor are connected by a recycle conduit 233; the first exchanger is fixedly connected with the first shell; the fan is fixedly connected with the first shell. The second exchanger, the heat radiation fan and the compressor are arranged in the second shell; the heat radiation fan is opposite to the second exchanger; the second exchanger is fixedly connected with the second shell; the heat dissipation fan is fixedly connected with the second shell; the compressor is fixedly connected with the second shell.
The interiors of the first exchanger, the second exchanger, the compressor and the circulation conduit 233 are filled with a medium which is easily evaporated and non-flammable, such as: freon, R410A. In the working process, a medium is changed into high-pressure gas through a compressor; then the heat is condensed and released through a second exchanger to become high-pressure liquid; then the high-pressure liquid is evaporated and absorbed heat by the first exchanger to become low-temperature and low-pressure gas, and finally the low-temperature and low-pressure gas returns to the compressor. Thereby, the cooling of the seal assembly by the auxiliary assembly 23 is completed. Meanwhile, since the surface temperature of the first exchanger is lower than the temperature of the sealing assembly, water vapor inside the sealing assembly can be condensed on the surface of the first exchanger, thereby realizing the maintenance of a dry environment inside the sealing assembly.
The above description of the embodiments is only for the understanding of the present invention. It should be noted that modifications could be made to the invention without departing from the principle of the invention, which would also fall within the scope of the claims of the invention.

Claims (10)

1. A high content specialized hypoxia workstation, comprising:
the observation device comprises a high content cell analyzer and a sealing component for maintaining a low oxygen environment for the high content cell analyzer;
the observation assemblies are respectively arranged on the top and the side wall of the sealing assembly;
and a supply device for providing a low-oxygen environment for the observation device and maintaining a low-temperature dry environment inside the observation device.
2. The high-content special low-oxygen workstation according to claim 1, wherein the sealing assembly comprises a top sealing structure, a side wall sealing structure and a bottom sealing structure; the bottom sealing structure and the side wall sealing structure are separated and the bottom of the side wall sealing structure is fixed; the top sealing structure and the side wall sealing structure are separated and the top of the side wall sealing structure is fixed.
3. The high-content special low-oxygen workstation according to claim 2, wherein the observation component comprises a first observation position, a second observation position and a third observation position; the first observation position and the second observation position are both arranged at the top of the sealing assembly; the third observation position is arranged on the side wall of the sealing component;
preferably, the first observation position is separated from the sealing assembly and fixed at the top of the sealing assembly;
preferably, the second observation position is separated from the sealing assembly and fixed at the top of the sealing assembly;
preferably, the third viewing position is separate from the sealing assembly and is fixed to a side wall of the sealing assembly.
4. The special high-content low-oxygen workstation according to claim 3, wherein the first observation position is separated from the top sealing structure and fixed on the top of the top sealing structure;
preferably, the second viewing position is separate from the top sealing structure and is fixed on the top of the top sealing structure;
preferably, the third viewing position is separate from the side wall sealing structure and is fixed on the side wall of the side wall sealing structure.
5. The high-content special low-oxygen workstation according to claim 2, wherein pipeline ports are arranged on the side wall of the sealing assembly; the pipeline port is separated from the sealing assembly and is fixed on the side wall of the sealing assembly;
preferably, the line port is separate from the sidewall seal structure and is fixed to the sidewall of the sidewall seal structure.
6. The high content specialized low oxygen workstation according to any one of claims 1 to 5, wherein the supply device comprises a control structure, a low oxygen supply assembly and an auxiliary assembly; the control structure is connected with the low oxygen supply assembly and the auxiliary assembly through leads.
7. The high-content special low-oxygen workstation according to claim 6, wherein the low-oxygen supply assembly comprises a gas cylinder, a pressure gauge, a gas valve and a gas guide pipe; the pressure gauge and the air valve are both arranged at the top of the air bottle; one end of the air valve, which is far away from the air bottle, is connected with the air guide pipe;
preferably, one end of the air duct, which is far away from the air valve, is connected with the sealing component;
preferably, the gas cylinder is a nitrogen gas cylinder.
8. The high-content special low-oxygen workstation according to claim 6, wherein the auxiliary components comprise an external circulation machine, an internal circulation machine and a circulation conduit; the circulating outer machine is connected with the circulating inner machine through a circulating conduit;
preferably, the circulation internal machine is arranged inside the sealing assembly; the outdoor circulation unit is disposed outside the sealing assembly.
9. The high-content special low-oxygen workstation according to claim 8, wherein the circulating indoor unit comprises a first shell, a first exchanger and a fan; the first exchanger and the fan are both arranged inside the first shell; the fan is opposite to the first exchanger;
preferably, the first exchanger is connected with an external circulation machine through a circulation conduit;
preferably, the first exchanger is separated from the first shell and fixed on the inner wall of the first shell;
preferably, the fan is separated from the first housing and fixed to an inner wall of the first housing.
10. The high-content special low-oxygen workstation according to claim 8 or 9, wherein the outdoor circulation unit comprises a second shell, a second exchanger, a heat dissipation fan and a compressor; the second exchanger, the heat dissipation fan and the compressor are arranged in the second shell; the heat dissipation fan is opposite to the second exchanger;
preferably, the second exchanger is connected to the circulation internal machine by a circulation conduit;
preferably, the second exchanger is separated from the second shell and fixed on the inner wall of the second shell;
preferably, the heat dissipation fan is separated from the second housing and fixed on the inner wall of the second housing;
preferably, the compressor is separated from the second housing and fixed to an inner wall of the second housing.
CN202211209091.0A 2022-09-30 2022-09-30 Special low oxygen workstation of high connotation Pending CN115948243A (en)

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Application Number Priority Date Filing Date Title
CN202211209091.0A CN115948243A (en) 2022-09-30 2022-09-30 Special low oxygen workstation of high connotation

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Application Number Priority Date Filing Date Title
CN202211209091.0A CN115948243A (en) 2022-09-30 2022-09-30 Special low oxygen workstation of high connotation

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CN115948243A true CN115948243A (en) 2023-04-11

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5887439A (en) * 1995-05-22 1999-03-30 Kotliar; Igor K. Hypoxic cleanroom systems for industrial applications
CN101466823A (en) * 2006-06-16 2009-06-24 三洋电机株式会社 Culture monitoring system
CN201623010U (en) * 2009-12-31 2010-11-03 北京七星华创电子股份有限公司 Low-oxygen control system
CN102328792A (en) * 2011-06-30 2012-01-25 王春录 Regulation system and method for closed storage environment
CN205139012U (en) * 2014-05-12 2016-04-06 赛洛米克斯股份有限公司 System for carry out automatic high intension cell formation of image
CN105955348A (en) * 2016-06-27 2016-09-21 中国特种设备检测研究院 Environment box of material universal test machine
CN207540253U (en) * 2017-09-30 2018-06-26 林金花 A kind of box microwave dryer
CN210765329U (en) * 2019-07-29 2020-06-16 西安医学院 Low-pressure and low-oxygen cell experimental device
US20200383342A1 (en) * 2018-02-27 2020-12-10 Daikin Industries, Ltd. Inside air control apparatus and container refrigeration apparatus including the same

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5887439A (en) * 1995-05-22 1999-03-30 Kotliar; Igor K. Hypoxic cleanroom systems for industrial applications
CN101466823A (en) * 2006-06-16 2009-06-24 三洋电机株式会社 Culture monitoring system
CN201623010U (en) * 2009-12-31 2010-11-03 北京七星华创电子股份有限公司 Low-oxygen control system
CN102328792A (en) * 2011-06-30 2012-01-25 王春录 Regulation system and method for closed storage environment
CN205139012U (en) * 2014-05-12 2016-04-06 赛洛米克斯股份有限公司 System for carry out automatic high intension cell formation of image
CN105955348A (en) * 2016-06-27 2016-09-21 中国特种设备检测研究院 Environment box of material universal test machine
CN207540253U (en) * 2017-09-30 2018-06-26 林金花 A kind of box microwave dryer
US20200383342A1 (en) * 2018-02-27 2020-12-10 Daikin Industries, Ltd. Inside air control apparatus and container refrigeration apparatus including the same
CN210765329U (en) * 2019-07-29 2020-06-16 西安医学院 Low-pressure and low-oxygen cell experimental device

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