CN204198730U - Anerobe, microaerobe and need carbonic acid gas bacterium culture bag - Google Patents
Anerobe, microaerobe and need carbonic acid gas bacterium culture bag Download PDFInfo
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- CN204198730U CN204198730U CN201420689871.4U CN201420689871U CN204198730U CN 204198730 U CN204198730 U CN 204198730U CN 201420689871 U CN201420689871 U CN 201420689871U CN 204198730 U CN204198730 U CN 204198730U
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Abstract
The utility model belongs to microbial technology field, is specifically related to a kind of anerobe, microaerobe and needs the culture bag of carbonic acid gas bacterium.The technical problems to be solved in the utility model is anaerobism, micro-aerobic and need the cultivation of carbonic acid gas microorganism and transport to provide a kind of new to select.The technical solution of the utility model is a kind of anerobe, microaerobe and needs carbonic acid gas bacterium culture bag, and this culture bag is the one end open of transparent soft bag, transparent soft bag, and opening is provided with closing device, transparent soft bag is at least provided with a breather valve.This culture bag can be used for cultivation and the transport of anaerobion, can Reusability, and support the use with gas tank, in gas tank, gaseous constituent and ratio can be prepared as required, and gas usage greatly reduces than rigid gas tank or incubator.Like this, with low cost, have broad application prospects.
Description
Technical field
The utility model belongs to microbial technology field, is specifically related to a kind of anerobe, microaerobe and needs the culture bag of carbonic acid gas bacterium.
Background technology
The cultivation of microorganism needs 4 large indispensable primary conditions: substratum (nutritive substance), temperature, atmosphere surrounding and potential of hydrogen.And the atmosphere surrounding needed for microorganism growth is roughly divided into a few class: aerobic, amphimicrobian, need carbonic acid gas, anaerobism, microaerophilic etc.This culture bag can be anerobe, microaerobe and the atmosphere surrounding that needs the cultivation of carbonic acid gas bacterium to provide special very expediently, is beneficial to the cultivation of these gas with various environmental demand microorganisms.
At present, on market the product of microorganism culturing atmosphere surrounding that provides of confession have about two classes: one is incubator, and two is aerogenesis bag+sealable plastics bag or canister.
Incubator is a kind of airtight incubator, the gas tank of external off-gas pump and preparation specific gas.After unpacking and putting into culture, close upper chamber door, then extract the air in casing out, then put the specific gas in gas tank into, so need repeatedly 3 times to guarantee that in case, air is fully changed, to ensure that atmosphere surrounding meets the requirements.There is following defect in this incubator:
1) the somewhat expensive side of occupation of land again of incubator, also will be equipped with large gas tank just occupation of land side more simultaneously;
2) waste is large, substitutes gas volume that gas consumes large; An incubator once can only use a kind of gas, needs the microorganism of different special gas just to difficult for if cultivate two or more simultaneously, such as, simultaneously cultivates anerobe and microaerobe is just not all right at an incubator; And clinical samples is not often simultaneously censorship, but must inoculate again sample in time puts into special gas environment and hatches, and ventilation of repeatedly bleeding is lost time (each spended time about half an hour), waste gas, waste of manpower.And incubator is bled, air interchanger is too complicated.If evening, Holidays, be also pretty troublesome for watchstander, because this equipment is not often in laboratory on duty, and the operation of non-microorganism lab assistant is not yet done, can be pretty troublesome.
Aerogenesis bag+sealable plastics bag or little case capsule: aerogenesis bag, chemical reaction aerogenesis, that one tears extraction, chemical reaction is there is after bag content ingress of air, specific gas environment in airtight plastics bag or little case capsule required for oxygen depleted generation, for the cultivation of peculiar microorganism.But also there is following defect in this capsule:
1) aerogenesis bag cost is higher, single use, and be import at present entirely, valid period is 1 year.The shorter stock of validity period, the external supply of material have trouble.Its inherent material rear discarded by single use aerogenesis bag has potential harm to environment.
2) in the plastics bag of sealing, place aerogenesis bag, due to the capabilities limits of aerogenesis bag process air, plastics bag can not be too large, generally can only place the agar plate of about 3 diameter 90mm.But aerogenesis bag but occupies certain area, block the situation (transparent plastic bag through sealing) of observing microorganism growth on dull and stereotyped interior nutrient agar; Aerogenesis bag is consuming the oxygen in plastics bag, and in plastics bag, gas volume reduces, and negative pressure makes aerogenesis bag and plastics bag and flat board stick together, and the position blocked by it to the observation of displacement aerogenesis bag is just very difficult.
3) state of Clinical practice is that user thinks that anaerobic gas generation bag is too expensive, widely uses and has any problem at present.
Utility model content
The technical problems to be solved in the utility model is for anerobe, microaerobe and needs the cultivation of carbonic acid gas bacterium and transport to provide one newly to select easily.
The technical solution of the utility model is a kind of anerobe, microaerobe and the culture bag needing carbonic acid gas bacterium, and this culture bag is the one end open of transparent soft bag, transparent soft bag, and opening is provided with closing device, transparent soft bag is at least provided with a breather valve.
Preferably, transparent soft bag is provided with two breather valves.
Further, Anaerobic indicator is also had in described culture bag.
Preferably, described closing device is pressure-sealing zipper or sealing clip.
Beneficial effect:
1) owing to being transparent soft bag, bleed excluding air can direct vision to whether fully (sack bleed fully show as sack be inwardly close together), when pouring into special gas can direct vision to the special gas poured into whether enough (slightly heaving), also avoid the waste of excessively inflating;
2) validity period is long, and difference requires that the gas-holder of gas can arbitrarily be changed, and the gas volume of consumption is little and very cheap; Do not need the aerogenesis bag utilizing chemical reaction; Special gas can entrust specialized factory to prepare on request, and the gas such as cultivating helicobacter pylori is just very special, and user can select different special gas gas-holder " to connect " culture bag soon as required;
3) gas consumed is few, and the consigned processing of gas and gas-holder is easy and with low cost;
4) culture bag can Reusability;
5) due to simple to operate, as long as bleed, inflate several seconds, cost is low, greatly can promote that clinical microbiology laboratory technique and scientific research are carried out, and for anerobe, microaerobe, needs carbonic acid gas bacterium, helicobacter pylori etc. to need the microorganism culturing of special gas to start a new situation.
Accompanying drawing explanation
Fig. 1 culture bag structural representation of the present utility model
Fig. 2 culture bag structural representation of the present utility model
Mark in figure: transparent soft bag 1, opening 2, breather valve 3, sealing clip 4, Anaerobic indicator 5, culture dish 6.
Embodiment
The utility model provides a kind of anaerobion culture bag, and this culture bag is the one end open of transparent soft bag, transparent soft bag, and opening is provided with closing device, transparent soft bag is at least provided with a breather valve.Closing device is set and is conducive to culture bag Reusability, convenient placement substratum and Anaerobic indicator etc.Breather valve is for connecting air pump.
Transparent soft bag one end open is used for the substratum having inoculated sample or bacterial strain to put into culture bag, namely culture bag is sealed by closing device after putting into.Then connect air pump by breather valve, the air in culture bag is extracted out.Connected the air pump of special gas needed for training objective microorganism again by this breather valve, gas is filled with in culture bag.
Preferably, transparent soft bag is provided with two breather valves, air pump can be connected respectively, one for air is extracted out in culture bag, one for passing into required gas.
Further, in described culture bag, also have Anaerobic indicator, be used to indicate gas-condition in band.
Preferably, described closing device is for being pressure-sealing zipper or sealing clip.
Embodiment
As depicted in figs. 1 and 2, the culture dish 6 having inoculated sample or bacterial strain is put into transparent soft bag 1 by opening 2, with sealing clip 4 by pouch seal; Breather valve 3 is connected rapidly in succession the pipe connecting of gas tank, vapor pipe is connected the fast interface of off-gas pump, open off-gas pump and bleed, till exhausting the air in bag, turn off off-gas pump, block the pipe connecting in culture bag, take off the interface departing from off-gas pump, open the gas tank valve of culturing micro-organisms desired gas, start aeration, slightly heave to soft bag and both can turn off gas tank valve stopping aeration, close breather valve, put into common incubator.
Preferably, as shown in Figure 2, also can arrange 2 breather valves on transparent soft bag, one connects off-gas pump by tracheae, and a gas tank being connected to culturing micro-organisms desired gas, first bleeds, then gas injection.
As shown in Figure 2, in order to better react the environmental aspect in culture bag, also in transparent soft bag, place Anaerobic indicator 5.
Claims (4)
1. anerobe, microaerobe and need carbonic acid gas bacterium culture bag, is characterized in that: this culture bag is the one end open of transparent soft bag, transparent soft bag, and opening is provided with closing device; Transparent soft bag is at least provided with a breather valve.
2. as claimed in claim 1 anerobe, microaerobe and need carbonic acid gas bacterium culture bag, is characterized in that: transparent soft bag is provided with two breather valves.
3. anerobe as claimed in claim 1 or 2, microaerobe and need the culture bag of carbonic acid gas bacterium, is characterized in that: also have Anaerobic indicator in described culture bag.
4. anerobe as claimed in claim 1, microaerobe and need the culture bag of carbonic acid gas bacterium, is characterized in that: described closing device is pressure-sealing zipper or sealing clip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420689871.4U CN204198730U (en) | 2014-11-14 | 2014-11-14 | Anerobe, microaerobe and need carbonic acid gas bacterium culture bag |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420689871.4U CN204198730U (en) | 2014-11-14 | 2014-11-14 | Anerobe, microaerobe and need carbonic acid gas bacterium culture bag |
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| CN204198730U true CN204198730U (en) | 2015-03-11 |
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| CN201420689871.4U Active CN204198730U (en) | 2014-11-14 | 2014-11-14 | Anerobe, microaerobe and need carbonic acid gas bacterium culture bag |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105420073A (en) * | 2015-12-28 | 2016-03-23 | 北京澳利文生物科技有限公司 | Anaerobe culture bag and method for culturing anaerobe by using anaerobe culture bag |
| CN109022256A (en) * | 2018-07-17 | 2018-12-18 | 广东药科大学附属第医院 | Anaerobic culturel bag with Anaerobic indicator |
| CN113817584A (en) * | 2021-08-30 | 2021-12-21 | 北京大学第一医院 | Anaerobic bacteria bedside culture detection system |
-
2014
- 2014-11-14 CN CN201420689871.4U patent/CN204198730U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105420073A (en) * | 2015-12-28 | 2016-03-23 | 北京澳利文生物科技有限公司 | Anaerobe culture bag and method for culturing anaerobe by using anaerobe culture bag |
| CN109022256A (en) * | 2018-07-17 | 2018-12-18 | 广东药科大学附属第医院 | Anaerobic culturel bag with Anaerobic indicator |
| CN109022256B (en) * | 2018-07-17 | 2022-05-13 | 广东药科大学附属第一医院 | Anaerobic culture bag with anaerobic indicator |
| CN113817584A (en) * | 2021-08-30 | 2021-12-21 | 北京大学第一医院 | Anaerobic bacteria bedside culture detection system |
| CN113817584B (en) * | 2021-08-30 | 2023-12-29 | 北京大学第一医院 | Anaerobic bacteria bedside culture detection system |
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