CN202786236U - Dialysis culture device of anaerobic bacteria for laboratory - Google Patents
Dialysis culture device of anaerobic bacteria for laboratory Download PDFInfo
- Publication number
- CN202786236U CN202786236U CN 201220446864 CN201220446864U CN202786236U CN 202786236 U CN202786236 U CN 202786236U CN 201220446864 CN201220446864 CN 201220446864 CN 201220446864 U CN201220446864 U CN 201220446864U CN 202786236 U CN202786236 U CN 202786236U
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- stainless steel
- dialysis
- laboratory
- culture device
- bottle stopper
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Abstract
A dialysis culture device of anaerobic bacteria for laboratory consists of a plastic cover, a conical bottle stopper, stainless steel tubes, sealing clamp rings, shanked stainless steel balls, a dialysis bag and a conical bottle, wherein the conical bottle is sealed by the conical bottle stopper; the stainless steel tubes penetrate through the conical bottle stopper; one end of each stainless steel tube is connected with the plastic cover; the other end of each stainless steel tube is connected with a shank of each shanked stainless steel ball; the dialysis bag is connected with spherical parts of the shanked stainless steel balls and clamped and fixed by the sealing clamp rings; and inoculating dial windows are formed in the stainless steel tubes. With the adoption of the stainless steel tubes, the dialysis culture device resists high temperature, is easy to store and difficult to corrode and overcomes the defect that the former dialysis culture device made of glass is easy to break; consumables are saved; the cost is lowered; the dialysis bag can be expanded sufficiently due to the support functions of the steel balls; a good environment is created for inoculation and culture of the bacteria; and the stainless steel balls are different in size so as to be suitable for different types of dialysis bags. The dialysis culture device overcomes the defects that the laboratory is easy to pollute, little toxin is produced, the operation is complicated formerly. The dialysis culture device can be used repeatedly, and materials can be saved for studies of the laboratory.
Description
Technical field
The utility model relates to use for laboratory anerobe dialysis culture field, is a kind of use for laboratory anerobe dialysis culturing device specifically.
Background technology
The dialysis culture technology is the main method that contemporary Anaerobic Bacteria is cultivated, and it is good to have a bacterial growth, can obtain purer cultured products, reduces the loss of nutritive substance, so make substratum can quilt the efficient advantage such as utilization.
Therefore aspect bacterial studies, collecting required meta-bolites is the basis of bacterial studies, also is the important stage, and can collect efficiently opsonigenous substances becomes experimental study a great problem to be solved is arranged.Though at present industrialized fermentor cultivation device is arranged, lack effective laboratory culture device, for the fundamental research in laboratory has brought great difficulty.
Anerobe (anaerobicbacteria) be a class under oxygen free condition than the good bacterium that in aerobic environment, grows, and can not air (18% oxygen) and (or) the epontic bacterium of solid medium under 10% gas concentration lwevel.
Dialysis culture is that microorganism culturing is wrapped up with dialysis membrane, and a kind of cultural method that makes the outside have fresh medium flowing.
Different dialysis culture methods is adopted in the most laboratory, but all have the meta-bolites amount few (only being about 15% ~ 30%) easily pollute, collect, substratum can not be fully utilized and the problem such as complicated operation, its major cause be in the culture apparatus between dialysis tubing and each pipeline in conjunction with undertighten, cause bacterium to enter substratum easily and cause polluting, thereby cause the bacterium raised growth in the Culture Medium Laboratory, consume nutritive ingredient, and then making the bacterium in the dialysis tubing can not get sufficient nutrient and cause malnutrition, meta-bolites is few.Bacterium in the substratum outside the dialysis tubing is because anaerobic state is poor, and the purpose product of generation is few, adds the impact of medium component, cause purpose product separation difficulty, the serious waste substratum, the simultaneously connection between original device each several part is complicated, is difficult to obtain satisfied effect.
Summary of the invention
In sum, the purpose of this utility model is to provide a kind of use for laboratory anerobe dialysis culturing device.
The purpose of this utility model realizes by following technology:
A kind of use for laboratory anerobe dialysis culturing device is comprised of plastic cover, taper bottle stopper, stainless steel tube, seal snap ring, band handle Stainless Steel Ball, dialysis tubing and Erlenmeyer flask, and Erlenmeyer flask seals by the taper bottle stopper; Stainless steel tube passes the taper bottle stopper, and the one end connects plastic cover, the shank of the other end connecting band handle Stainless Steel Ball; Dialysis tubing is connected with ball-type position with the handle Stainless Steel Ball, and fixing by the seal snap ring chucking.
Above-mentioned stainless steel tube is provided with the inoculation dial window.
Advantage of the present utility model and beneficial effect:
1, the utility model is applicable to the cultivation of laboratory anaerobic type bacterium, customer service in the past the laboratory easily pollute, produce poison less, the shortcoming of complicated operation, and can Reusability, be the research economical with materials in laboratory.
2, the stainless steel tube that adopts of the utility model, high temperature resistant, be easy to preserve, and also not perishable, be that microorganism is not had influential material, avoided adopting glass material to hold breakable shortcoming in the past, saved consumptive material, reduce cost.
3, the high-temperature resistance plastice lid is adopted in the utility model upper end, inoculation is convenient, the tube wall of stainless steel tube is provided with the inoculation dial window, be convenient to inoculation, the internal thread connecting band handle Stainless Steel Ball of stainless steel column lower end, the supporting role of steel ball can make dialysis tubing expand fully, can create good environment for inoculation and the cultivation of bacterium, and the difference of the size of Stainless Steel Ball, can be fit to dissimilar dialysis tubings.
4, the employed seal snap ring of the utility model can effectively make things convenient for the connection between implement device, and is simple to operate, and sealing thoroughly.
Description of drawings
Fig. 1 is structural representation of the present utility model.
Among the figure: the 1-plastic cloth 2-gauze 3-taper bottle stopper 4-stainless steel tube 5-inoculation dial window 6-seal snap ring 7-band handle Stainless Steel Ball 8-9-of culturing room dialysis tubing 10-Erlenmeyer flask
Embodiment
Below in conjunction with accompanying drawing the utility model is described further:
As shown in Figure 1, a kind of use for laboratory anerobe dialysis culturing device is comprised of plastic cover 1, taper bottle stopper 3, stainless steel tube 4, seal snap ring 6, band handle Stainless Steel Ball 7, dialysis tubing 9 and Erlenmeyer flask 10, and Erlenmeyer flask 10 is by 3 sealings of taper bottle stopper; Stainless steel tube 4 passes taper bottle stopper 3, and the one end connects plastic cover 1, the shank of the other end connecting band handle Stainless Steel Ball 7; Dialysis tubing 9 is connected with ball-type position with handle Stainless Steel Ball 7, and fixing by seal snap ring 6 chuckings; Stainless steel tube 4 is provided with inoculation dial window 5.
Embodiment
Two stainless steel tubes 4 are passed taper bottle stopper 3, and osculum is outside, and large mouthful inwardly; Osculum stretches out about the about 2 ~ 3cm of bottle stopper, and is unsuitable long in order to avoid the impact wrapping.Have inoculation dial window 5 on the stainless steel tube 4, be convenient to inoculation.Use two seal snap rings 6 with the ball-type end of handle Stainless Steel Ball 7 dialysis tubing 9, and by on the large mouth that is fixed in stainless steel tube 4 one ends with handle Stainless Steel Ball 7 pommel screw threads, invade bubble in culturing room 8; The osculum end of stainless steel tube 4 has screw thread, uses high voltage bearing plastic cover 1 to cover, should not be too tight during high pressure, can make the gas overflowing in the dialysis tubing 9, and after the inoculation it is covered tightly, in order to avoid pollute bacterial classification.
Because the supporting role with the ball-type end of handle Stainless Steel Ball 7 can make dialysis tubing 9 expand fully, for good environment is created in inoculation and the cultivation of bacterium, and because stainless steel tube 4 exposed junctions are young, be difficult for during inoculation polluting, at last gauze 2 covered 10 mouthfuls of Erlenmeyer flasks.
Claims (2)
1. use for laboratory anerobe dialysis culturing device, be comprised of plastic cover (1), taper bottle stopper (3), stainless steel tube (4), seal snap ring (6), band handle Stainless Steel Ball (7), dialysis tubing (9) and Erlenmeyer flask (10), it is characterized in that: Erlenmeyer flask (10) seals by taper bottle stopper (3); Stainless steel tube (4) passes taper bottle stopper (3), and the one end connects plastic cover (1), the shank of the other end connecting band handle Stainless Steel Ball (7); Dialysis tubing (9) is connected with the ball-type position of band handle Stainless Steel Ball (7), and fixing by seal snap ring (6) chucking.
2. a kind of use for laboratory anerobe dialysis culturing device as claimed in claim 1 is characterized in that: described stainless steel tube (4) is provided with inoculation dial window (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220446864 CN202786236U (en) | 2012-09-04 | 2012-09-04 | Dialysis culture device of anaerobic bacteria for laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220446864 CN202786236U (en) | 2012-09-04 | 2012-09-04 | Dialysis culture device of anaerobic bacteria for laboratory |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202786236U true CN202786236U (en) | 2013-03-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220446864 Expired - Fee Related CN202786236U (en) | 2012-09-04 | 2012-09-04 | Dialysis culture device of anaerobic bacteria for laboratory |
Country Status (1)
| Country | Link |
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| CN (1) | CN202786236U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560688A (en) * | 2013-10-23 | 2015-04-29 | 中国科学院沈阳应用生态研究所 | Device and method for culturing hybrid microorganisms |
-
2012
- 2012-09-04 CN CN 201220446864 patent/CN202786236U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560688A (en) * | 2013-10-23 | 2015-04-29 | 中国科学院沈阳应用生态研究所 | Device and method for culturing hybrid microorganisms |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130313 Termination date: 20140904 |
|
| EXPY | Termination of patent right or utility model |