CN202766520U - Timed feeding device for methylotrophic bacteria fermentation - Google Patents
Timed feeding device for methylotrophic bacteria fermentation Download PDFInfo
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- CN202766520U CN202766520U CN 201220489149 CN201220489149U CN202766520U CN 202766520 U CN202766520 U CN 202766520U CN 201220489149 CN201220489149 CN 201220489149 CN 201220489149 U CN201220489149 U CN 201220489149U CN 202766520 U CN202766520 U CN 202766520U
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- carbon source
- pipe
- peristaltic pump
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- computer
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Abstract
The utility model relates to a timed feeding device for methylotrophic bacteria fermentation. The timed feeding device aims to solve the problems that a carbon source cannot be continuously added in the microbial fermentation and cultivation process at present, and more poly beta hydroxybutyrate is inconveniently obtained. One end of a carbon source addition tube extends into a fermentation tank, the other end of the carbon source addition tube is connected with a peristaltic pump, the peristaltic pump is connected with a feeding bottle, a timer is arranged on the peristaltic pump, a flow meter is arranged at a carbon source inlet of the carbon source addition tube, an analog signal output end of the flow meter is connected with an analog signal input end of an amplifying circuit, a signal output end of the amplifying circuit is connected with an analog signal input end of an A/D (analog-to-digital) conversion circuit, a digital signal output end of the A/D conversion circuit is connected with a digital signal input end of a computer, and a display signal output end of the computer is connected with a display signal input end of a display. The timed feeding device is applicable to fermentation and cultivation of methylotrophic bacteria.
Description
Technical field
The utility model relates to a kind of timing feed supplementing device, is specifically related to a kind of timing feed supplementing device for the methylotrophic bacteria fermentation.
Background technology
Carbon source is the class nutrition source that the required carbon of microbial nutrition can be provided in the microorganism growth process.The bacterium of methyl Pseudomonas can utilize methane, methyl alcohol, methylamine, the multiple methyl compound of methyl halide to make carbon source through fermentation and produce poly beta-hydroxybutyrate.Its character has many similarities with the synthetic plastics that uses at present, to environment without any pollution, can be for the preparation of good degradable plastics.In order to obtain more poly beta-hydroxybutyrate, need to be to the bacterium of methyl Pseudomonas the cultivation of continuously fermenting.And at present, can't in the microbial fermentation culturing process, realize continuous interpolation carbon source, brought inconvenience for the more poly beta-hydroxybutyrate of acquisition.
Summary of the invention
The purpose of this utility model is can't realize continuous interpolation carbon source in order to solve at present in the microbial fermentation culturing process, brought inconvenient problem for the more poly beta-hydroxybutyrate of acquisition, and then a kind of timing feed supplementing device for the methylotrophic bacteria fermentation is provided.
The technical solution of the utility model is: a kind of timing feed supplementing device for the methylotrophic bacteria fermentation comprises fermentor tank, regularly feed supplementing device also comprises peristaltic pump, the feed supplement bottle, timer, fermented liquid flows out pipe, fermented liquid flows out valve, carbon source is added pipe, pipe connecting, under meter, amplifying circuit, the A/D change-over circuit, computer and indicating meter, the upper end of fermentor tank has carbon source and adds mouth, the lower end of fermentor tank has the fermentation broth stream outlet, one end of carbon source interpolation pipe passes carbon source interpolation mouth and stretches in the fermentor tank, the other end that carbon source is added pipe is connected with peristaltic pump, peristaltic pump is connected with the feed supplement bottle by pipe connecting, set timer is on peristaltic pump, fermented liquid flows out pipe and is arranged in the fermentation broth stream outlet, and be communicated with the inner chamber of fermentor tank by fermentation broth stream outlet, fermented liquid flows out valve and is arranged on the fermented liquid outflow pipe, the carbon source ingress that carbon source is added pipe is provided with under meter, and the analog signal output of described under meter is connected with the input end of analog signal of amplifying circuit; The signal output part of amplifying circuit is connected with the input end of analog signal of A/D change-over circuit; The digital signal output end of described A/D change-over circuit is connected with the digital signal input end of computer; The display output terminal of computer is connected with the display input terminus of indicating meter.
Described timing feed supplementing device also comprises carbon source interpolation valve, and carbon source is added valve and is arranged on the carbon source interpolation pipe, and carbon source is added the top that valve is positioned under meter.
Described timing feed supplementing device also comprises data-carrier store, and the data signal of described data-carrier store inputs or outputs end and is connected with data signal output or the input terminus of computer.
The utility model compared with prior art has following effect: 1. peristaltic pump of the present utility model injects the carbon source in the feed supplement bottle in the fermentor tank, and regularly realize the continuous adding of carbon source by timer, give to obtain more poly beta-hydroxybutyrate and bring great convenience.2. the utility model can also realize that by under meter accuracy of detection is high, and is real-time to detecting the online detection of carbon source addition in the microbial fermentation culturing process.3. the utility model is simple in structure, and is with low cost, manufactures easily.
Description of drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
Embodiment one: present embodiment is described in conjunction with Fig. 1, present embodiment comprises fermentor tank 1, regularly feed supplementing device also comprises peristaltic pump 2, feed supplement bottle 3, timer 4, fermented liquid flows out pipe 5, fermented liquid flows out valve 6, carbon source is added pipe 7, pipe connecting 8, under meter 9, amplifying circuit 10, A/D change-over circuit 11, computer 12, indicating meter 13, carbon source is added valve 16 and data-carrier store 17, the upper end of fermentor tank 1 has carbon source and adds a mouthful 1-1, the lower end of fermentor tank 1 has fermentation broth stream outlet 1-2, one end of carbon source interpolation pipe 7 passes carbon source interpolation mouthful 1-1 and stretches in the fermentor tank 1, the other end that carbon source is added pipe 7 is connected with peristaltic pump 2, peristaltic pump 2 is connected with feed supplement bottle 3 by pipe connecting 8, timer 4 is arranged on the peristaltic pump 2, fermented liquid flows out pipe 5 and is arranged on the fermentation broth stream outlet 1-2, and be communicated with the inner chamber of fermentor tank 1 by fermentation broth stream outlet 1-2, fermented liquid flows out valve 6 and is arranged on the fermented liquid outflow pipe 5, the carbon source ingress that carbon source is added pipe 7 is provided with under meter 9, carbon source is added valve 16 and is arranged on the carbon source interpolation pipe 7, and carbon source is added the top that valve 16 is positioned under meter 9, and the analog signal output of described under meter 9 is connected with the input end of analog signal of amplifying circuit 10; The signal output part of amplifying circuit 10 is connected with the input end of analog signal of A/D change-over circuit 11; The digital signal output end of described A/D change-over circuit 11 is connected with the digital signal input end of computer 12; The display output terminal of computer 12 is connected with the display input terminus of indicating meter 13, and the data signal of data-carrier store 17 inputs or outputs end and is connected with data signal output or the input terminus of computer 12.
The utility model arranges under meter in the ingress of fermentor tank, in whole carbon source adding procedure, addition is added up, then flow signal is amplified and mould/number A/D conversion, and numerary signal inputed in the computer, computer converts to flow, thereby obtains the detection data of flow, thereby realizes the online carbon source addition that detects in the microbial fermentation culturing process.
Use procedure of the present utility model is:
Microorganism passes through fermentor cultivation, from the inoculation of first day set time, the later identical time of every day, timer starts and the work of driving peristaltic pump, feed supplement 200mL in the fermentor tank, the feed supplement of this 200mL is detected and is controlled by under meter, simultaneously from the fermented liquid of fermentation broth stream outlet output 200mL, this 200mL fermented liquid of output detects by external measuring cup, keeps amount constant of solution in the fermentor tank with this.
Claims (3)
1. one kind is used for the timing feed supplementing device that methylotrophic bacteria ferments, it comprises fermentor tank (1), it is characterized in that: regularly feed supplementing device also comprises peristaltic pump (2), feed supplement bottle (3), timer (4), fermented liquid flows out pipe (5), fermented liquid flows out valve (6), carbon source is added pipe (7), pipe connecting (8), under meter (9), amplifying circuit (10), A/D change-over circuit (11), computer (12) and indicating meter (13), the upper end of fermentor tank (1) has carbon source and adds mouthful (1-1), the lower end of fermentor tank (1) has fermentation broth stream outlet (1-2), one end of carbon source interpolation pipe (7) passes carbon source interpolation mouthful (1-1) and stretches in the fermentor tank (1), the other end that carbon source is added pipe (7) is connected with peristaltic pump (2), peristaltic pump (2) is connected with feed supplement bottle (3) by pipe connecting (8), timer (4) is arranged on the peristaltic pump (2), fermented liquid flows out pipe (5) and is arranged in the fermentation broth stream outlet (1-2), and be communicated with the inner chamber of fermentor tank (1) by fermentation broth stream outlet (1-2), fermented liquid flows out valve (6) and is arranged on the fermented liquid outflow pipe (5), the carbon source ingress that carbon source is added pipe (7) is provided with under meter (9), and the analog signal output of described under meter (9) is connected with the input end of analog signal of amplifying circuit (10); The signal output part of amplifying circuit (10) is connected with the input end of analog signal of A/D change-over circuit (11); The digital signal output end of described A/D change-over circuit (11) is connected with the digital signal input end of computer (12); The display output terminal of computer (12) is connected with the display input terminus of indicating meter (13).
2. a kind of timing feed supplementing device for methylotrophic bacteria fermentation according to claim 1, it is characterized in that: described timing feed supplementing device also comprises carbon source interpolation valve (16), carbon source is added valve (16) and is arranged on the carbon source interpolation pipe (7), and carbon source is added the top that valve (16) is positioned under meter (9).
3. detect online the device of carbon source addition in the microbial fermentation culturing process according to claim 2, it is characterized in that: described timing feed supplementing device also comprises data-carrier store (17), and the data signal of described data-carrier store (17) inputs or outputs end and is connected with data signal output or the input terminus of computer (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220489149 CN202766520U (en) | 2012-09-24 | 2012-09-24 | Timed feeding device for methylotrophic bacteria fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220489149 CN202766520U (en) | 2012-09-24 | 2012-09-24 | Timed feeding device for methylotrophic bacteria fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202766520U true CN202766520U (en) | 2013-03-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220489149 Expired - Fee Related CN202766520U (en) | 2012-09-24 | 2012-09-24 | Timed feeding device for methylotrophic bacteria fermentation |
Country Status (1)
| Country | Link |
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| CN (1) | CN202766520U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224965A (en) * | 2013-03-27 | 2013-07-31 | 中国人民解放军军事医学科学院生物工程研究所 | Method for producing pyrroloquinoline quinine through microbial fermentation and fermentation medium used in same |
-
2012
- 2012-09-24 CN CN 201220489149 patent/CN202766520U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224965A (en) * | 2013-03-27 | 2013-07-31 | 中国人民解放军军事医学科学院生物工程研究所 | Method for producing pyrroloquinoline quinine through microbial fermentation and fermentation medium used in same |
| CN103224965B (en) * | 2013-03-27 | 2015-04-01 | 中国人民解放军军事医学科学院生物工程研究所 | Method for producing pyrroloquinoline quinine through microbial fermentation and fermentation medium used in same |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Shuai Inventor after: Wang Baoqing Inventor after: You Qing Inventor before: Zhang Shuai Inventor before: Wang Baoqing |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: ZHANG SHUAI WANG BAOQING TO: ZHANG SHUAI WANG BAOQING YOU QING |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130306 Termination date: 20130924 |