CN2647864Y - Reactor for in vitro blood vessel culture - Google Patents
Reactor for in vitro blood vessel culture Download PDFInfo
- Publication number
- CN2647864Y CN2647864Y CNU032501706U CN03250170U CN2647864Y CN 2647864 Y CN2647864 Y CN 2647864Y CN U032501706 U CNU032501706 U CN U032501706U CN 03250170 U CN03250170 U CN 03250170U CN 2647864 Y CN2647864 Y CN 2647864Y
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- Prior art keywords
- blood vessel
- incubator
- reservoir
- pressure
- pressure controller
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Abstract
The utility model relates to a culture reactor of exosomatic blood vessels. A pressure controller A, a pressure bumper B, a germfree isolating box D and a reservoir F are interconnected by pipes to form a peripheral passage. The utility model is characterized in that the output end of the pressure controller is connected with a three-limb tube 1 by the pressure bumper, the detecting end of the three-limb tube is connected with a pressure detector C and the germfree isolating box D, an incubator E is arranged in the germfree isolating box, a plurality pairs of removable blood vessel joints 3 are kept parallel with the incubator, each pair of blood vessel joint is communicated with the pipes and connected with the input end of the reservoir F in a high position through the pipes, the output end of the reservoir is connected with the input end of the pressure controller. The utility model has the advantages that the culture reactor can conveniently process cell planting, provide cell with a good and steady culture environment, and can simulate the blood flowing situation of internal blood vessels at the same time and provide the culture of blood vessels with needed mechanical stimulation. The need of building different diameters of blood vessels can be met by the replacement of the joints.
Description
Technical field
The utility model relates to a kind of extracorporeal blood vessel culture apparatus, specifically is a kind of the be endotheliocyte that is applicable to external structure engineering blood vessel or artificial blood vessel, biological vascular reaction device of the special development of smooth muscle cell plantation.This reactor partly is replaced with the rheological properties that the parallel plate flow cavity also can be used for detecting cell with culture apparatus.
Technical background
Be used to make up the bio-reactor of blood vessel in the past, and all adopted rigid material to make joint.Because rigidity joint quality is hard, corner angle appear, and when cultivating under long-time, high pressure condition, often cause the distortion of sampling point local organization, cause blood vessel injury.In addition, this class transmitter also has some shortcomings, for example, all is to make of metal, and complex process is used inconvenience, and especially the blood vessel shank needs special factory process to make, and cost is higher, and is inconvenient according to making up the big or small joint of changing of blood vessels caliber.
Summary of the invention
The purpose of this utility model is just in order to overcome the above-mentioned shortcoming that prior art exists, provide a kind of by changing the blood vessel joint, make up the blood vessel of different tube diameters, be applicable to external structure engineering blood vessel or artificial blood vessel's endotheliocyte, the extracorporeal blood vessel cultivation reaction unit of smooth muscle cell plantation.
The utility model adopts to such an extent that technical scheme is such, and promptly a kind of extracorporeal blood vessel is cultivated reactor, becomes the circulation path by pressure controller, pulsation dampener, sterile cupboard and reservoir by pipe connection; It is characterized in that: the output terminal of pressure controller connects Y-tube by pulsation dampener, the detection termination pressure detector of Y-tube, its output terminal connects sterile cupboard by pipeline, has incubator in the sterile cupboard, incubator has been arranged in parallel some to disconnectable blood vessel joint, the inlet side of every pair of blood vessel joint is connected by the Y-tube outside outer threeway of pipe connecting and incubator and the sterile cupboard, the end that goes out of every pair of blood vessel joint connects the outer switch of incubator by pipe connecting, and by pipeline be in elevated reservoir inlet side and be connected, the end that goes out of reservoir is connected with the inlet side of pressure controller.
Described incubator, reservoir, pulsation dampener, aseptic shielded box are that pmma material makes.
The utility model is rational in infrastructure owing to described structure has, cost is low, easy to make, do not need complicated processing requirement, characteristics such as simple to operate, applied range.
Description of drawings
Accompanying drawing is the structural representation of structure embodiment of the present utility model.
Referring to accompanying drawing: pressure control device, B--pressure buffer device, C--pressure-detecting device, D--constant temperature are isolated sterile cupboard, E--culture apparatus, F--device for storing liquid among the A--figure, and 1,2--three-way device, 3--blood vessel joint, the 4--blood vessel, the 5--switch that make up.
Embodiment
As shown in the figure.A is a pressure controller, is made up of pulsate pump and trip switch, conduit.
Among the embodiment, the output terminal of pressure controller A communicates with pressure buffer device B by conduit.Pulsation dampener joins by conduit and three-way device 1, and the detecting end of threeway 1 communicates with pressure-detecting device C by conduit.Pipeline enters aseptic shielded box, and respectively by four pipelines and threeway 2 blood vessel incubators, threeway 2 may command liquid pass in and out and inject cell mixture and somatomedin etc. in blood vessel, and 5 is the switching arrangement of the blood vessel the other end, the turnover of may command liquid.Blood vessel incubator E can make up a blood vessel separately also can make up a plurality of blood vessels simultaneously, and blood vessel joint 3 can be by more bringing the blood vessel that makes up different tube diameters, and 4 is the blood vessel that will make up.E is replaced with the parallel plate flow cavity with the blood vessel incubator, then can carry out the cytorheology experiment.Reservoir F is the device for storing liquid of keeping certain height.Pulsation dampener B, aseptic shielded box D and reservoir F place 37 ℃ of CO2 incubators in the whole device.
The utility model is according to the vascular pulsation principle, adopt all kinds of silica gel volution joints and transfusion device joint, several making blood vessel joints of the transfusion needle after emery wheel processing, to reduce the stimulation that the blood vessel kiss is connect the position, can satisfy the needs that make up the different tube diameters blood vessel by changing joint.Simultaneously, this reactor adopts " integral body splits " principle, between the several means structurally is to be interconnected and to split each other, thereby can reaches the purpose that makes up blood vessel on function.Therefore, the utility model has overcome the deficiency of prior art, can conveniently carry out cell seeding, provides good stable culture environment to cell, and vascular flow situation in the energy analogue body provides blood vessel to cultivate required mechanical stimulation simultaneously.And can reach and make up convenient, the purpose efficiently of blood vessel.
Claims (3)
1, a kind of extracorporeal blood vessel is cultivated reactor, becomes the circulation path by pressure controller (A), pulsation dampener (B), aseptic shielded box (D) and reservoir (F) by pipe connection; It is characterized in that: the output terminal of pressure controller connects Y-tube (1) by pulsation dampener, the detection termination pressure detector (C) of Y-tube, its output terminal connects by pipeline isolates sterile cupboard (D), isolate in the sterile cupboard and have incubator (E), incubator has been arranged in parallel some to disconnectable blood vessel joint (3), the inlet side of every pair of blood vessel joint is connected by threeway (2) outside pipe connecting and the incubator and the Y-tube (1) outside the sterile cupboard, the end that goes out of every pair of blood vessel joint connects switch (5) outside the incubator by pipe connecting, and by pipeline be in elevated reservoir (F) inlet side and be connected, the end that goes out of reservoir is connected with the inlet side of pressure controller.
2, extracorporeal blood vessel according to claim 1 is cultivated reactor, it is characterized in that: incubator (E), reservoir (F), pressure buffer (B), aseptic shielded box are that pmma material makes.
3, extracorporeal blood vessel according to claim 1 is cultivated reactor, it is characterized in that: pulsation dampener (B), constant temperature isolate sterile cupboard (D) and reservoir (F) places the CO2 incubator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU032501706U CN2647864Y (en) | 2003-08-28 | 2003-08-28 | Reactor for in vitro blood vessel culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU032501706U CN2647864Y (en) | 2003-08-28 | 2003-08-28 | Reactor for in vitro blood vessel culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2647864Y true CN2647864Y (en) | 2004-10-13 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU032501706U Expired - Fee Related CN2647864Y (en) | 2003-08-28 | 2003-08-28 | Reactor for in vitro blood vessel culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2647864Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372661B (en) * | 2008-03-18 | 2011-09-07 | 北京航空航天大学 | Adjustable pouring type vascular tissue engineering reactor having rotating cultivation cavity |
| CN101400779B (en) * | 2006-03-06 | 2011-10-05 | 三洋电机株式会社 | Incubator for isolator |
| CN101486965B (en) * | 2009-02-13 | 2012-06-20 | 中国人民解放军第三军医大学第一附属医院 | Tissue engineering blood vessel bionic incubator |
| CN103266182A (en) * | 2013-05-30 | 2013-08-28 | 中山大学 | Method and device for regulating tower plate type perfusion bioreactor |
| CN115232750A (en) * | 2022-09-22 | 2022-10-25 | 海迈医疗科技(苏州)有限公司 | Sterile culture bag and method of use thereof |
-
2003
- 2003-08-28 CN CNU032501706U patent/CN2647864Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101400779B (en) * | 2006-03-06 | 2011-10-05 | 三洋电机株式会社 | Incubator for isolator |
| CN101372661B (en) * | 2008-03-18 | 2011-09-07 | 北京航空航天大学 | Adjustable pouring type vascular tissue engineering reactor having rotating cultivation cavity |
| CN101486965B (en) * | 2009-02-13 | 2012-06-20 | 中国人民解放军第三军医大学第一附属医院 | Tissue engineering blood vessel bionic incubator |
| CN103266182A (en) * | 2013-05-30 | 2013-08-28 | 中山大学 | Method and device for regulating tower plate type perfusion bioreactor |
| CN103266182B (en) * | 2013-05-30 | 2015-04-15 | 中山大学 | Method and device for regulating tower plate type perfusion bioreactor |
| CN115232750A (en) * | 2022-09-22 | 2022-10-25 | 海迈医疗科技(苏州)有限公司 | Sterile culture bag and method of use thereof |
| CN115232750B (en) * | 2022-09-22 | 2023-02-24 | 海迈医疗科技(苏州)有限公司 | Sterile culture bag and method of use |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20041013 Termination date: 20120828 |