CN203999633U - A kind of vascular bodies external stress culture apparatus and culture systems - Google Patents
A kind of vascular bodies external stress culture apparatus and culture systems Download PDFInfo
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- CN203999633U CN203999633U CN201420215681.9U CN201420215681U CN203999633U CN 203999633 U CN203999633 U CN 203999633U CN 201420215681 U CN201420215681 U CN 201420215681U CN 203999633 U CN203999633 U CN 203999633U
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Abstract
Vascular bodies external stress culture apparatus and a culture systems, this device is coaxially provided with web member in culture tank both sides; The first mounting block is coaxially housed in web member, and web member is coaxially fixed with fastening piece outward, the second mounting block is housed in coaxial in fastening piece, coaxially has respectively third and fourth through hole in first and second mounting block; Between first and second mounting block, be provided with sealing-ring; Blood vessel pipe connecting stretches in culture tank through fourth hole, sealing-ring and third through-hole.This device can improve the alignment of blood vessel and blood vessel pipe connecting, and good airproof performance.In this system, surge flask forms loop by input, output channel and vascular bodies external stress culture apparatus; Input channel is provided with constant flow pump and flow sensor, and output channel is provided with pressure transmitter; Gas regulating valve, constant flow pump, flow sensor and pressure transmitter are connected with PC respectively.This system can accurately be controlled the pressure in culturing process and flow, improves the reliability of cultivation results.
Description
Technical field
The utility model belongs to blood vessel vitro culture field, is specifically related to a kind of vascular bodies external stress culture apparatus and culture systems.
Background technology
In normal physiological environment, under the arteries effect in blood pressure and blood flow all the time.The caused mechanical stress of blood pressure and blood flow, i.e. hoop stress and shearing stress, all the time vasoactive wall.When these mechanical stresses change, will cause blood vessel to produce the adaptations of function and structure.Under hypertension condition, reinventing of blood vessel may be exactly a kind of adaptation reaction of the mechanical stress to increasing.
Arterial Rings In Vitro blood vessel culture apparatus occurred since the nineties in last century, nineteen ninety-five Nathalie Bardy etc. has designed organ culture model outside a kind of vascular bodies, the thoracic aorta of rabbit is carried out to vitro culture, artery survival is more than 8 days, it is the vascular pattern of comparatively successful vitro culture, but it is simple that its device shows slightly, can only cultivate arteries (the Bardy N of large animal, Karilon GL, Merval R, et al.Differential effectsof pressure and flow on DNA synthesis and protein synthesis and on fibronectionexpression by arteries in a novel organ culture system, Cire Res, 1995, 77:684.).The people such as Robert in 1996 utilize dog arteria carotis communis and people's saphena to carry out blood vessel vitro culture, its research emphasis is the hydrodynamicpressure of pulsation, and there is defect in the design of blood vessel culture chamber, can not stand the cultivation of long period, and can not control steady state flow (Robert F Labadie, et al.Pulsatile perfusion system for exvivo investigation of biochemical pathways in intact vascular tissue.Am J Physiol, 1996,270 (Heart Circ Physiol39): H760.).Calendar year 2001 Han Haichao etc. has designed a set of similar blood vessel vitro culture system, be used for studying the Carotid contractive reactivity of vitro culture pig under the effect of pulsating nature high pressure, this system also can only be simulated pulsatile blood flow, and, cannot realize computer control (the Han HC of pressure and flow, Ku DN.Contractile responses in arteries subjected to hypertensivepressure in seven-day.Organ culture, Annals of Biomedical Engineering, 2001,29:467.).Above Object of Development is the arteries of large animal.In the cultivation of animalcule blood vessel, also there is culture tank and the linking conduit bad problem that interfixes, for example, fixing its alignment of linking conduit in culture tank two ends poor (be on space not on a line), and there is the shortcoming that not easy and leakage are installed.
Utility model content
The purpose of this utility model is to provide a kind of vascular bodies external stress culture apparatus and culture systems, and this culture apparatus not only can improve the alignment of the blood vessel pipe connecting at blood vessel and its two ends, and good airproof performance; This culture systems can accurately be controlled the pressure in culturing process and flow, improves the reliability of cultivation results.
For achieving the above object, the technical solution adopted in the utility model is:
A vascular bodies external stress culture apparatus, comprises culture tank, and culture tank is provided with upper cover, above covers and is provided with air filter; The both sides of culture tank are respectively equipped with web member, and the web member of culture tank both sides is coaxial; On the axis of web member, offer the first through hole, the first mounting block is housed in the first through hole, on the axis of the first mounting block, offer third through-hole; The outer, coaxial of web member is fixed with fastening piece, offers the second through hole on the axis of fastening piece, and the second mounting block is housed in the second through hole, on the axis of the second mounting block, offers fourth hole; Between the first mounting block and the second mounting block, be provided with sealing-ring; Blood vessel pipe connecting passes successively fourth hole, sealing-ring and third through-hole and stretches in culture tank from the outside of fastening piece.
The described external diameter of blood vessel pipe connecting and the ratio of the internal diameter of blood vessel to be cultivated are (1～1.2): 1.
Distance between the web member of described culture tank both sides is (2.5～3.5) with the length ratio of blood vessel to be cultivated: 1.
The external diameter of described third through-hole and the internal diameter of fourth hole and blood vessel pipe connecting matches, and the internal diameter of sealing-ring is greater than the external diameter of blood vessel pipe connecting.
Described web member and fastening piece are threaded connection coaxial fixing.
The outside of described web member is provided with outside screw, is provided with groove in fastening piece, and groove is provided with the internal thread mating with outside screw.
The first described mounting block and the second mounting block are T shape pipe, and T shape pipe comprises the boss of managing and being arranged on pipe one end, and the external diameter of boss is greater than the external diameter of pipe;
Described sealing-ring is clipped between the boss of the first mounting block and the boss of the second mounting block;
The first described through hole is notch cuttype through hole, and the first through hole comprises the first hole, rank matching with boss external diameter and the second-order hole matching with pipe external diameter;
The second described through hole and the external diameter of pipe match, and the boss of the second mounting block is positioned at groove.
The external diameter of described sealing-ring is (0.8～1) with the ratio of the external diameter of boss: 1.
The sealing-ring thickness sum of the boss thickness that the degree of depth in the first described hole, rank is twice and 0.5～0.7 times.
The vascular bodies external stress culture systems that described vascular bodies external stress culture apparatus builds, comprises surge flask, and surge flask is provided with gas inlet, nutrient solution entrance and nutrient solution outlet, and gas inlet is provided with gas regulating valve; Nutrient solution outlet is connected with the blood vessel pipe connecting of vascular bodies external stress culture apparatus one side by input channel, and the blood vessel pipe connecting of opposite side is connected with nutrient solution entrance by output channel, forms loop; Input channel is provided with constant flow pump and flow sensor, and output channel is provided with pressure transmitter; The top of vascular bodies external stress culture apparatus is provided with laser source, and below is provided with photoreceptor; Gas regulating valve, constant flow pump, flow sensor, pressure transmitter and laser source are connected with PC respectively.
With respect to prior art, the beneficial effects of the utility model are:
The vascular bodies external stress culture apparatus that the utility model provides, because the first through hole is opened on the axis of web member, fastening piece is coaxially fixed on web member outside, and the second through hole is opened on the axis of fastening piece, so the first through hole and the second through hole are coaxial, and first be equipped with the first mounting block in through hole, the second mounting block is housed in the second through hole, on the axis of the first mounting block, offer third through-hole, on the axis of the second mounting block, offer fourth hole, so third through-hole and fourth hole are coaxial, between the first mounting block and the second mounting block, be provided with sealing-ring, blood vessel pipe connecting passes fourth hole successively from the outside of fastening piece, sealing-ring and third through-hole also stretch in culture tank, blood vessel pipe connecting is spacing by third through-hole and fourth hole, can be good at the alignment of maintenance and third through-hole and fourth hole, in addition because the web member of culture tank both sides is coaxial, therefore the blood vessel pipe connecting of culture tank both sides also can reach the requirement of height alignment, when carrying out blood vessel culture experiment, the two ends of blood vessel are enclosed within respectively the two ends of coaxial two relative blood vessel pipe connectings in culture tank, can meet blood vessel requirement to alignment in culturing process of various thicknesses, improve the activity of blood vessel and the reliability of experimental result.In addition owing between the first mounting block and the second mounting block, sealing-ring being set, when fastening piece is fixed on web member, the second mounting block can compress to the first mounting block, thereby causes sealing-ring to be extruded distortion, reach good sealing effectiveness, can prevent that nutrient solution is to outward leakage.This fixed form of web member and fastening piece in the utility model, had both solved the connection of blood vessel and blood vessel pipe connecting and fixing alignment problem, had solved again the sealing problem of blood vessel pipe connecting.And the utility model also has advantages of simple in structure, easy to install and usely, can shorten the time that blood vessel is articulated to blood vessel pipe connecting, make nutrient solution ne-leakage in blood vessel culturing process.In addition upper cover and on air filter can also preventing dust pollute and the sterility of maintain process.
Further, in the vascular bodies external stress culture apparatus that the utility model provides, the external diameter of blood vessel pipe connecting is (1～1.2) with the ratio of the internal diameter of blood vessel to be cultivated: 1, while like this vascular cuffing being contained on blood vessel pipe connecting, blood vessel can not come off on the one hand, another is convenient also can not be supportted excessive and cause blood vessel injury, thus avoid affecting experiment effect.In the time need to carrying out the blood vessel cultivation of different thicknesses, only need to change the blood vessel pipe connecting of corresponding external diameter and the first mounting block of corresponding internal diameter and the second mounting block, can in same set of device, complete the blood vessel culture experiment process of different inner diameters.
Further, in the vascular bodies external stress culture apparatus that the utility model provides, distance between the web member of culture tank both sides is (2.5～3.5) with the length ratio of blood vessel to be cultivated: 1, two blood vessel pipe connectings penetrate culture tank from the web member of culture tank both sides respectively, blood vessel is fixed to its position of rear adjustment, be located at the mid-way of culture tank, the length ratio that the blood vessel pipe connecting of the length of blood vessel and its both sides stretches into the part in culture tank is about 1:1:1, be convenient to blood vessel to be fixed on blood vessel pipe connecting so on the one hand, it is very little that blood vessel pipe connecting stretches into the swing of the part in culture tank on the other hand, can guarantee good alignment, and confirm through test, the experimental result obtaining under such length condition is also more reliable.
Further, in the vascular bodies external stress culture apparatus that the utility model provides, the internal diameter of third through-hole and fourth hole is zero allowance with the external diameter of blood vessel pipe connecting and coordinates, can eliminate the swing of blood vessel pipe connecting, the internal diameter of sealing-ring is greater than the external diameter of blood vessel pipe connecting simultaneously, guarantee that sealing-ring can easily pass blood vessel pipe connecting in the situation that not being extruded, and can play certain tightness to blood vessel pipe connecting when being extruded.
Further, in the vascular bodies external stress culture apparatus that the utility model provides, web member and fastening piece are threaded connection, and can pass through the fastening degree of screw thread condition, and first mounting block and the second mounting block be T shape pipe, the first through hole is notch cuttype through hole, its size coordinates with the T shape pipe zero allowance of the first mounting block, the second through hole coordinates with the external diameter zero allowance of the pipe of the second mounting block, the boss of the second mounting block is positioned at groove, sealing-ring is clipped between the boss of the first mounting block and the boss of the second mounting block, the external diameter of sealing-ring is (0.8～1) with the ratio of the external diameter of boss: 1, the degree of depth on the first hole, rank is the boss thickness of twice and the sealing-ring thickness sum of 0.5～0.7 times, after like this in the first through hole of the T of the first mounting block shape pipe threading web member, in its outer end, put again sealing-ring, thickness and the degree of depth that is less than the first hole, rank due to this T shape pipe and sealing-ring, like this when the T of the second mounting block shape pipe is contained in fitting nut and when fastening piece is tightened on web member, the boss of the T shape pipe of the second mounting block can enter the first hole, rank, during extruding, sealing-ring deforms to both sides inside and outside it, so, the effect of fastening blood vessel pipe connecting is played again in the effect of playing sealing, what make blood vessel pipe connecting fixedly becomes simple and convenient, and dynamics is just right.
The vascular bodies external stress culture systems that the utility model provides, by surge flask, input channel, form the circulation loop of nutrient solution with vascular bodies external stress culture apparatus together with output channel, and control gas regulating valve, constant flow pump, flow sensor, pressure transmitter and laser source by PC, make pressure and flow in system to carry out real-time accurate control by computer, saved the step that regulates manually afterload, greatly lower the manual intervention load in culturing process, improved the reliability of cultivation results.
Accompanying drawing explanation
The structure explosive view of the vascular bodies external stress culture apparatus that Fig. 1 provides for the utility model;
The structure explosive view of the connection portion of the vascular bodies external stress culture apparatus that Fig. 2 provides for the utility model;
The connection portion of the vascular bodies external stress culture apparatus that Fig. 3 provides for the utility model analyse and observe explosive view;
The structural representation of the vascular bodies external stress culture apparatus that Fig. 4 provides for the utility model;
Wherein: 1 is culture tank, 2 is web member, 3 is the first mounting block, 4 is sealing-ring, 5 is fastening piece, 6 is blood vessel pipe connecting, 7 is the first through hole, 8 is outside screw, 9 is internal thread, 10 is the second through hole, 11 is the second mounting block, 12 is third through-hole, 13 is fourth hole, 14 is groove, 15 is pipe, 16 is boss, 17 is the first hole, rank, 18 is second-order hole, 19 is upper cover, 20 is air filter, 21 is gas regulating valve, 22 is surge flask, 23 is laser source, 24 is pressure transmitter, 25 is flow sensor, 26 is constant flow pump, 27 is liquid inlet, 28 is nutrient solution entrance, 29 is nutrient solution outlet, 30 is photoreceptor, 31 is PC, 32 is output channel, 33 is input channel, 34 is vascular bodies external stress culture apparatus, 35 is blood vessel to be cultivated.
Embodiment
If the alignment of the blood vessel pipe connecting at blood vessel and two ends is poor in arteries culturing process, the sweeping style of nutrient solution (perfusate) in cultivating blood vessel can change, this can cause certain damage to cultivating blood vessel, changes the result of blood vessel culture experiment.It is relevant with the internal diameter of cultivating blood vessel that the alignment of the blood vessel pipe connecting at blood vessel and its two ends requires, and less its requirement of internal diameter is higher.The internal diameter of rat artery blood vessel (≤1mm) is less more than 8～10 times than the arteries internal diameter that has at present cultivation animal, therefore requires very high alignment.And the fixed form of current blood vessel pipe connecting and culture tank, great majority are to adopt revising the fixing and sealing material of the rubber hose of the certain wall thickness of overcoat on pipe connecting as culture tank and modification pipe connecting, in elastomeric material use procedure, soft or hard degree cannot be consistent, certainly will cause the poor reason of alignment in fixation procedure, the external diameter size of revising pipe connecting can not arbitrarily change, the blood vessel that cannot coordinate different inner diameters, necessarily very poor if this mode is used in the cultivation of the rat artery blood vessel that internal diameter is less effect.
Therefore the utility model has carried out the improvement of following two aspects: 1. the pressure of system and flow are realized computer control; 2. improve the blood vessel pipe connecting at blood vessel two ends and the fixed form of culture tank, improve alignment and stopping property, to adapt to the cultivation of the myocardium vessel of the various internal diameters that comprise isolated rat arteries.
Below in conjunction with accompanying drawing, the utility model is described in further detail.
Referring to Fig. 1 to Fig. 3, the vascular bodies external stress culture apparatus that the utility model provides, comprises culture tank 1, and culture tank 1 is provided with upper cover 19, and upper cover 19 is provided with air filter 20.The both sides of culture tank 1 are respectively equipped with web member 2, and the web member 2 of culture tank 1 both sides is coaxial; Distance between the web member 2 of culture tank 1 both sides is (2.5～3.5) with the length ratio of blood vessel 35 to be cultivated: 1.On the axis of web member 2, offer in the first through hole 7, the first through holes 7 the first mounting block 3 is housed, on the axis of the first mounting block 3, offer third through-hole 12.The first mounting block 3 is T shape pipe, and T shape pipe comprises pipe 15 and the boss 16 that is arranged on pipe 15 one end, and the external diameter of boss 16 is greater than the external diameter of pipe 15; The first through hole 7 is notch cuttype through hole, and the first through hole 7 comprises the first hole, rank 17 matching with boss 16 external diameters and the second-order hole 18 matching with pipe 15 external diameters.The outside of web member 2 is provided with outside screw 8, is provided with groove 14 in fastening piece 5, and groove 14 is provided with the internal thread 9 mating with outside screw 8, and web member 2 and fastening piece 5 are threaded connection coaxial fixing.On the axis of fastening piece 5, offer in the second through hole 10, the second through holes 10 the second mounting block 11 is housed, on the axis of the second mounting block 11, offer fourth hole 13.The second mounting block 11 is T shape pipe, and T shape pipe comprises pipe 15 and the boss 16 that is arranged on pipe 15 one end, and the external diameter of boss 16 is greater than the external diameter of pipe 15; The second through hole 10 matches with the external diameter of pipe 15, and the boss of the second mounting block 11 is positioned at groove 14.Between the boss 16 of the first mounting block 3 and the boss 16 of the second mounting block 11, be provided with sealing-ring 4; The external diameter of sealing-ring 4 is (0.8～1) with the ratio of the external diameter of boss 16: 1.The degree of depth on the first hole, rank 17 is boss 16 thickness of twice and the sealing-ring 4 thickness sums of 0.5～0.7 times.Blood vessel pipe connecting 6 passes successively fourth hole 13, sealing-ring 4 and third through-hole 12 and stretches in culture tank 1 from the outside of fastening piece 5.The external diameter of the internal diameter of third through-hole 12 and fourth hole 13 and blood vessel pipe connecting 6 matches, and the internal diameter of sealing-ring 4 is greater than the external diameter of blood vessel pipe connecting 6.The external diameter of blood vessel pipe connecting 6 is (1～1.2) with the ratio of the internal diameter of blood vessel 35 to be cultivated: 1.
Referring to Fig. 4, the vascular bodies external stress culture systems that the utility model provides, comprises surge flask 22, and surge flask 23 is provided with gas inlet 27, nutrient solution entrance 28 and nutrient solution outlet 29, and gas inlet 27 is provided with gas regulating valve 21; Nutrient solution outlet 29 is connected with the blood vessel pipe connecting 6 of vascular bodies external stress culture apparatus 34 1 sides by input channel 33, and the blood vessel pipe connecting 6 of opposite side is connected with nutrient solution entrance 28 by output channel 32, forms loop; Input channel 33 is provided with constant flow pump 26 and flow sensor 25, and output channel 32 is provided with pressure transmitter 24; The top of vascular bodies external stress culture apparatus 34 is provided with laser source 23, and below is provided with photoreceptor 30; Gas regulating valve 21, constant flow pump 26 (BT100-1L, Lange constant flow pump company limited, China), flow sensor 25, pressure transmitter 24 are connected with PC 31 respectively with laser source 23.Input channel and output channel are stainless steel, and the internal diameter of its external diameter and blood vessel pipe connecting matches.
The utility model in use, all pipelines of vascular bodies external stress culture systems and assembly are passed through to the aseptic sterilization of high temperature, in super clean bench, carry out assembly and connection, then fresh arteria carotis communis is taken out from postanesthetic rat, move to super clean bench, under stereoscope, repair and rinse, then rapidly by the colligation of blood vessel two ends on the blood vessel pipe connecting on the both sides in culture tank, make whole culture systems form a path, nutrient solution is driven by constant flow pump, now in culture tank and surge flask, divided and be clipped to into 25ml and 75ml nutrient solution [DMEM, 10% calf serum (HyClone, Logan, UT), microbiotic].Whole vascular bodies external stress culture systems is displaced in incubator, 37 ℃ and pass to 5%CO2 and the condition of the gas mixture of 95% air (volume fraction) under cultivate.In culturing process, can by PC, set vascular pressure (± 2mmHg) and the flow (± 0.2mL) of each time period in blood vessel culturing process, the control of intravascular pressure is controlled by the gas regulating valve on pressure transmitter and surge flask.Flow detection and control are what by the detection of flow sensor and the rotating speed of constant flow pump, to realize, and the structure of T shape pipe can be used for regulating the mobile pulse height of nutrient solution.In culture tank, the outer liquid of the cultivation of blood vessel carries out gaseous interchange by air filter (0.2 μ m filter membrane) with the external world.Can be according to the external diameter (± 3 μ m) that is arranged on laser position finding instrument on culture dish and measures in real time (comprising laser source and photoreceptor) blood vessel in whole experimentation.Culture systems medium vessels can be cultivated 3 days (more than the blood vessels of 3 days, cultivate and need to change the inside and outside nutrient solution of perfusion at the 3rd day) under different pressures.Can collect and detect the concentration of Ang II in the inside and outside liquid of perfusion.Cultivating blood vessel can fix by original position, further carries out morphologic detection.
The utility model adopts the constant flow pump of PC, pressure transmitter, flow sensor and Miniature precision to realize the control to the flow of system and pressure.In the fixed form of blood vessel pipe connecting and culture tank, for the difference of blood vessel pipe connecting outside dimension, adopt T shape pipe and the seamless cooperation of blood vessel pipe connecting of the different sizes of internal diameter, and T shape pipe is engaged in web member by seamless, T shape pipe outer end adds sealing-ring again and fastening piece is suitably fixed, so both solve the connection of blood vessel, blood vessel pipe connecting and culture tank and fixing alignment problem, solved again sealing problem.
The utility model can be cultivated according to the blood vessel of different inner diameters, different sites, the arterial vascular cultivation problem of different animals have been solved, blood vessel articulate with fixed form on the method used with the culture apparatus of previous literature report compare, cultivate blood vessel and there is good alignment, operate easier, can save the time of half, greatly improve the activity of blood vessel.While owing to having used computer control unit, has been saved the step that regulates manually afterload in the past in the control of pressure and flow, has greatly lowered the manual intervention load in culturing process.
The vascular bodies external stress culture systems that the utility model provides is totally-enclosed system, and the control of pressure is controlled by the mixed gas pressurization of the system of entering and rotating speed and the pressure transmitter of constant flow pump, and flow is controlled by rotating speed and the flow sensor of constant flow pump.The gas regulating valve of controlling mixed gas when the force value that system detected when PC does not conform to set force value increases air inlet or reduces air inlet; When the flux values that system detected when PC does not conform to set flux values, control constant flow pump and improve rotating speed or reduce rotating speed, carry out like this stable of the flow of Controlling System and pressure, can also put into practice the variation of the different pressure of different time and flow simultaneously.
The connection of culture tank and blood vessel pipe connecting and fixedly used T-socket in the utility model, the external diameter (because different arteries has different internal diameters) that can adapt on the one hand different blood vessel pipe connectings, such culture tank can be done the cultivation of multiple intra-arterial blood vessel, another aspect, the use of T shape pipe can make the axial symmetry of blood vessel pipe connecting very good, because the internal diameter of the external diameter of the internal diameter of the external diameter of blood vessel pipe connecting and T shape pipe and T shape pipe and the first through hole of web member is all zero allowance, coordinate, eliminated the swing of blood vessel pipe connecting.After in the first through hole of the T shape pipe threading web member as the first mounting block, in its outer end, put again sealing-ring, thickness and the degree of depth that is less than the first hole, rank due to this T shape pipe and sealing-ring, like this when the T shape pipe as the second mounting block is contained in fitting nut and when fastening piece is tightened on web member, boss as the T shape pipe of the second mounting block can enter the first hole, rank, during extruding, sealing-ring deforms to both sides inside and outside it, so, the effect of fastening blood vessel pipe connecting is played again in the effect of playing sealing, what make blood vessel pipe connecting fixedly becomes simple and convenient, and dynamics is just right.Particularly, when the external diameter of sealing-ring is identical with boss external diameter and coordinate with the first hole, rank zero allowance, during extruding, seal washer is merely able to the Direction distortion (center of circle direction of sealing-ring) to blood vessel pipe connecting, better to the fastening effect of blood vessel pipe connecting like this.
Claims (10)
1. a vascular bodies external stress culture apparatus, is characterized in that: comprise culture tank (1), culture tank (1) is provided with upper cover (19), and upper cover (19) is provided with air filter (20); The both sides of culture tank (1) are respectively equipped with web member (2), and the web member (2) of culture tank (1) both sides is coaxial; On the axis of web member (2), offer the first through hole (7), the first mounting block (3) is housed in the first through hole (7), on the axis of the first mounting block (3), offer third through-hole (12); The outer, coaxial of web member (2) is fixed with fastening piece (5), on the axis of fastening piece (5), offer the second through hole (10), the second mounting block (11) is housed in the second through hole (10), on the axis of the second mounting block (11), offers fourth hole (13); Between the first mounting block (3) and the second mounting block (11), be provided with sealing-ring (4); Blood vessel pipe connecting (6) passes successively fourth hole (13), sealing-ring (4) and third through-hole (12) and stretches in culture tank (1) from the outside of fastening piece (5).
2. vascular bodies external stress culture apparatus according to claim 1, is characterized in that: the external diameter of described blood vessel pipe connecting (6) is (1～1.2) with the ratio of the internal diameter of blood vessel to be cultivated (35): 1.
3. vascular bodies external stress culture apparatus according to claim 1, is characterized in that: the distance between the web member (2) of described culture tank (1) both sides is (2.5～3.5) with the length ratio of blood vessel to be cultivated (35): 1.
4. vascular bodies external stress culture apparatus according to claim 1, it is characterized in that: the internal diameter of described third through-hole (12) and fourth hole (13) and the external diameter of blood vessel pipe connecting (6) match, and the internal diameter of sealing-ring (4) is greater than the external diameter of blood vessel pipe connecting (6).
5. according to the vascular bodies external stress culture apparatus described in any one in claim 1-4, it is characterized in that: described web member (2) and fastening piece (5) are threaded connection coaxial fixing.
6. vascular bodies external stress culture apparatus according to claim 5, it is characterized in that: the outside of described web member (2) is provided with outside screw (8), in fastening piece (5), be provided with groove (14), groove (14) is provided with the internal thread (9) mating with outside screw (8).
7. vascular bodies external stress culture apparatus according to claim 6, it is characterized in that: described the first mounting block (3) and the second mounting block (11) are T shape pipe, T shape pipe comprises pipe (15) and is arranged on the boss (16) of pipe (15) one end, and the external diameter of boss (16) is greater than the external diameter of pipe (15);
Described sealing-ring (4) is clipped between the boss (16) of the first mounting block (3) and the boss (16) of the second mounting block (11);
Described the first through hole (7) is notch cuttype through hole, and the first through hole (7) comprises the first hole, rank (17) matching with boss (16) external diameter and the second-order hole (18) matching with pipe (15) external diameter;
Described the second through hole (10) matches with the external diameter of pipe (15), and the boss of the second mounting block (11) is positioned at groove (14).
8. vascular bodies external stress culture apparatus according to claim 7, is characterized in that: the ratio of the external diameter of the external diameter of described sealing-ring (4) and boss (16) is (0.8～1): 1.
9. vascular bodies external stress culture apparatus according to claim 7, is characterized in that: sealing-ring (4) the thickness sum of boss (16) thickness that the degree of depth in the first described hole, rank (17) is twice and 0.5～0.7 times.
10. the vascular bodies external stress culture systems building according to vascular bodies external stress culture apparatus described in any one in claim 1-9, it is characterized in that: comprise surge flask (22), surge flask (23) is provided with gas inlet (27), nutrient solution entrance (28) and nutrient solution outlet (29), and gas inlet (27) are provided with gas regulating valve (21); Nutrient solution outlet (29) is connected with the blood vessel pipe connecting (6) of vascular bodies external stress culture apparatus (34) one sides by input channel (33), the blood vessel pipe connecting (6) of opposite side is connected with nutrient solution entrance (28) by output channel (32), forms loop; Input channel (33) is provided with constant flow pump (26) and flow sensor (25), and output channel (32) is provided with pressure transmitter (24); The top of vascular bodies external stress culture apparatus (34) is provided with laser source (23), and below is provided with photoreceptor (30); Gas regulating valve (21), constant flow pump (26), flow sensor (25), pressure transmitter (24) and laser source (23) are connected with PC (31) respectively.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103952307A (en) * | 2014-04-29 | 2014-07-30 | 中国人民解放军第四军医大学 | In-vitro stress culture device for blood vessel, culture system and culture method |
| CN113512497A (en) * | 2021-08-12 | 2021-10-19 | 浙江大学 | 3D printing culture tank, culture system and detection system for in-vitro intestinal living body culture and application |
| CN115232750A (en) * | 2022-09-22 | 2022-10-25 | 海迈医疗科技(苏州)有限公司 | Sterile culture bag and method of use thereof |
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2014
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103952307A (en) * | 2014-04-29 | 2014-07-30 | 中国人民解放军第四军医大学 | In-vitro stress culture device for blood vessel, culture system and culture method |
| CN103952307B (en) * | 2014-04-29 | 2016-01-20 | 中国人民解放军第四军医大学 | A kind of vascular bodies external stress culture apparatus and culture systems and cultural method |
| CN113512497A (en) * | 2021-08-12 | 2021-10-19 | 浙江大学 | 3D printing culture tank, culture system and detection system for in-vitro intestinal living body culture and application |
| CN115232750A (en) * | 2022-09-22 | 2022-10-25 | 海迈医疗科技(苏州)有限公司 | Sterile culture bag and method of use thereof |
| CN115232750B (en) * | 2022-09-22 | 2023-02-24 | 海迈医疗科技(苏州)有限公司 | Sterile culture bag and method of use |
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