CN220424015U - Connecting device for perfusion in animal experiment - Google Patents
Connecting device for perfusion in animal experiment Download PDFInfo
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- CN220424015U CN220424015U CN202321036683.7U CN202321036683U CN220424015U CN 220424015 U CN220424015 U CN 220424015U CN 202321036683 U CN202321036683 U CN 202321036683U CN 220424015 U CN220424015 U CN 220424015U
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- 230000010412 perfusion Effects 0.000 title claims abstract description 57
- 238000002474 experimental method Methods 0.000 title claims abstract description 48
- 241001465754 Metazoa Species 0.000 title claims abstract description 41
- 210000000709 aorta Anatomy 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 16
- 238000001802 infusion Methods 0.000 claims abstract description 14
- 230000002572 peristaltic effect Effects 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000002861 polymer material Substances 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 229910001220 stainless steel Inorganic materials 0.000 claims description 2
- 239000010935 stainless steel Substances 0.000 claims description 2
- 210000000056 organ Anatomy 0.000 abstract description 15
- 210000001557 animal structure Anatomy 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 7
- 238000010171 animal model Methods 0.000 abstract description 5
- 230000023555 blood coagulation Effects 0.000 abstract description 4
- 239000011159 matrix material Substances 0.000 abstract description 4
- 230000007774 longterm Effects 0.000 abstract 1
- 238000011161 development Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 210000004204 blood vessel Anatomy 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 208000031873 Animal Disease Models Diseases 0.000 description 1
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 1
- 206010070863 Toxicity to various agents Diseases 0.000 description 1
- 238000011558 animal model by disease Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Abstract
The utility model discloses a connecting device for perfusion in animal experiments, which comprises a transfusion pipeline and a connecting pipeline; the method is characterized in that: the infusion pipeline comprises a connecting layer, a tissue connecting end is arranged on the left side of the connecting layer, and a pipeline connecting section is arranged on the right side of the connecting layer; the connecting layer left side is connected layer, anchor clamps layer, fixed layer from interior to exterior, connecting layer right side intercommunication connecting line, connecting layer and connecting line between be equipped with connection structure, connection structure be the quick connect port of pipeline link, fixed layer one side intercommunication connecting layer, the clearance between opposite side and the connecting layer is the anchor clamps layer, connecting line include single way pipeline or double-circuit pipeline. The problems that in animal experiments, particularly in perfusion experiments, due to the body type of experimental animals, the aorta of an organ is short, so that the time spent on connecting the aorta with a perfusion pipeline in the perfusion process is increased, and the blood coagulation in the organ is caused by the overlong connecting time, so that the perfusion effect is influenced are solved; when the animal organ decellularization experiment and the animal organ matrix culture experiment are carried out, the stability of the connection was checked during the long-term decellularization and culture.
Description
Technical Field
The utility model relates to the field of biology, in particular to the field of animal experiments, and particularly relates to a connecting device for perfusion in animal experiments.
Background
Animal experiments (Animal Experiment) refer to the scientific research carried out in laboratory using animals in order to obtain new knowledge about biology, medicine, etc., or to solve specific problems. Animal experiments must be performed by or under the direction of trained, academic or professional staff.
Animal experiments as scientific experimental objects greatly promote the development of life sciences. With the rapid advance of bioscience, it is recognized that pollution problems have become not only important social problems of food, population, old people and the like, but also animal survival problems living on earth, such as industrial pollution, food pollution, drug toxicity and the like, directly affect human health, and research on the problems is finally necessarily clarified and solved through animal experiments (including development of animal disease models and the like).
Although some animal models other than cells, tissues, and genetic materials are currently used for scientific research and teaching experiments, these models and materials cannot mimic and replace the complex physiological environment of the human or animal body, and thus, experiments using living animals are still required to further promote the life science development related to human and animal health.
Meanwhile, animal experiments are widely applied to the fields of biology, livestock and veterinary, and the like, the quality of the animal experiments directly influences the results of scientific researches and the quality of medicine production, the control level of microorganism and the quality is one of important marks of the quality and the standardization degree, and the control quality of the microorganism and the animal experiments is ensured and the animal experiments are required to be periodically detected by a certain program.
In animal experiments, particularly in perfusion experiments, due to the body type of experimental animals, the aorta of the organ is short, so that the time spent on connecting the aorta with a perfusion pipeline in the perfusion process is increased, and the blood coagulation in the organ can be caused by the overlong connecting time, so that the perfusion effect is influenced; the needle of the transfusion device which is used to be used is too large in diameter difference between the diameter of the needle of the transfusion device and the diameter of the aorta in the perfusion process of organs, so that perfusion solution is leaked, and the perfusion effect and the perfusion efficiency are affected; when the animal organ decellularization experiment and the animal organ matrix culture experiment are carried out, the stability of connection is tested in the long-time decellularization and culture process.
Disclosure of Invention
The technical problem to be solved by the utility model is that in animal experiments, particularly in perfusion experiments, due to the body type of experimental animals, the aorta of the organ is short, so that the time spent on connecting the aorta with a perfusion pipeline in the perfusion process is increased, and the blood coagulation in the organ can be caused by the overlong connecting time, thereby influencing the perfusion effect; the needle of the transfusion device which is used to be used is too large in diameter difference between the diameter of the needle of the transfusion device and the diameter of the aorta in the perfusion process of organs, so that perfusion solution is leaked, and the perfusion effect and the perfusion efficiency are affected; when the animal organ decellularization experiment and the animal organ matrix culture experiment are carried out, the stability of connection is tested in the long-time decellularization and culture process.
In order to solve the above-mentioned technical problems, the utility model adopts the following technical means:
a connecting device for animal experiment perfusion, comprises a transfusion pipeline and a connecting pipeline; the method is characterized in that: the infusion pipeline comprises a connecting layer, a tissue connecting end is arranged on the left side of the connecting layer, and a pipeline connecting section is arranged on the right side of the connecting layer; the connecting layer left side is connected layer, anchor clamps layer, fixed layer from interior to exterior, connecting layer right side intercommunication connecting line, connecting layer and connecting line between be equipped with connection structure, connection structure be the quick connect port of pipeline link, fixed layer one side intercommunication connecting layer, the clearance between opposite side and the connecting layer is the anchor clamps layer, connecting line include single way pipeline or double-circuit pipeline.
The utility model provides the connecting device for animal experiment perfusion by changing the connecting mode in the perfusion process, which is convenient for realizing the animal experiment perfusion.
Preferably, the utility model further adopts the technical scheme that:
the infusion pipeline and the connecting pipeline can be designed in different sizes according to different application scenes, and the fixation of blood vessels with different positions and different diameters is met.
The utility model discloses a single way pipeline with double-circuit pipeline one side be the quick-connect male joint, be equipped with the screw thread with infusion tube connection one side outside, the nut inboard is equipped with the screw thread, the pipeline link outside is equipped with the screw thread and the nut inboard is equipped with the screw thread structure and matches each other.
The connecting layer, the clamp layer, the fixing layer and the connecting pipeline are all made of stainless steel or high polymer materials.
The front section of the clamp layer is provided with four separated elastic pieces, the four elastic pieces form a certain angle with the connecting layer, and the rear parts of the four elastic pieces are fixedly connected by round tubes; the front part of the fixing layer is unthreaded, the rear part is connected with the connecting layer through threads, and the surface of the fixing layer is provided with anti-skid threads.
The diameter of the port of the tissue connecting end is lower than the pipe diameter of the infusion pipeline, the port of the pipeline connecting end is a quick-connection female head, and the port of the pipeline connecting end can be connected with a single-way pipeline or a double-way pipeline.
The infusion pipeline and the connecting pipeline are sterilized by alcohol soaking and high-temperature high-pressure modes.
The single-way pipeline comprises a single-way connector and a single-way pipe orifice.
The two-way pipeline comprises a two-way connector, a first pipe orifice and a second pipe orifice.
Four shrapnel at the front section of the clamp layer can be opened or closed, and the aorta is fixed by the four shrapnels.
The peristaltic pump is arranged on the infusion tube and provides power for the solution in the tube by extruding the infusion tube.
The utility model solves the problems that in animal experiments, particularly in perfusion experiments, due to the body type of experimental animals, the aorta of the organ is short, so that the time spent by connecting the aorta with a perfusion pipeline in the perfusion process is increased, and the blood coagulation in the organ can be caused by the overlong connecting time, thereby influencing the perfusion effect; in the perfusion process of organs, the needle head of the transfusion device used to practice can cause the leakage of perfusion solution due to the too large difference between the diameter of the transfusion device needle head and the current difference between the diameter of the transfusion device needle head and the aorta, thereby affecting the perfusion effect and the perfusion efficiency; when the animal organ decellularization experiment and the animal organ matrix culture experiment are carried out, the stability of connection is tested in the long-time decellularization and culture process.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present utility model.
Fig. 2 is a schematic diagram of a single pipeline structure according to an embodiment of the present utility model.
Fig. 3 is a schematic diagram of a two-way pipeline according to an embodiment of the present utility model.
Reference numerals illustrate: 1-a connection layer; 2-a clamp layer; 3-a fixed layer; 4-a pipeline connecting end; a 5-tissue junction; 6-a single pipeline; 7-two-way pipeline.
Detailed Description
The utility model will be further illustrated with reference to the following examples.
Specific example 1:
referring to fig. 1, the connecting device for perfusion in animal experiments according to the present utility model can be used to ensure efficient operation in the perfusion process of animal experimental organs.
Connecting a transfusion tube with a single pipeline (6), screwing a screw cap, then inserting the single pipeline (6) on a pipeline connecting end (4) of the connecting layer (1), connecting the single pipeline with the single pipeline, sleeving an aorta of an animal organ at a tissue connecting end (5) port of the connecting layer (1), rotating the fixing layer (3) to enable the fixing layer to advance, deforming a clamping piece of the clamp layer (2), fixing the aorta at the tissue connecting end (5), installing the transfusion tube on a peristaltic pump, opening the peristaltic pump according to requirements, adjusting the speed, and pumping solution into the organ.
Specific example 2:
referring to fig. 1, the connection device for perfusion in animal experiments according to the present utility model can ensure the mixed perfusion of two solutions in the perfusion process of animal experimental organs.
Connecting a perfusion tube with a two-way pipeline (7), screwing a screw cap, then inserting the two-way pipeline (7) on a pipeline connecting end (4) of the connecting layer (1) to be connected with the perfusion tube, sleeving an aorta of an animal organ at a tissue connecting end (5) port of the connecting layer (1), rotating the fixing layer (3) to advance the perfusion tube, deforming a clamping piece of the clamp layer (2), fixing the aorta at the tissue connecting end (5), installing the perfusion tube on a peristaltic pump, opening the peristaltic pump according to requirements, and adjusting the speed to enable two solutions to be pumped into the organ according to the ratio.
Since the above is only a specific embodiment of the present utility model, the present utility model is not limited thereto, and the application fields thereof include, but are not limited to, animal perfusion experiments, and also include in vitro tissue perfusion, development and verification of materials such as artificial biological blood vessels, and the like. Equivalent changes or substitutions of technical features of the present technical solution will occur to those skilled in the art, and are encompassed within the scope of the present utility model.
Claims (10)
1. A connecting device for animal experiment perfusion comprises a transfusion pipeline and a connecting pipeline; the method is characterized in that: the infusion pipeline comprises a connecting layer, a tissue connecting end is arranged on the left side of the connecting layer, and a pipeline connecting section is arranged on the right side of the connecting layer; the connecting layer left side is connected layer, anchor clamps layer, fixed layer from interior to exterior, connecting layer right side intercommunication connecting line, connecting layer and connecting line between be equipped with connection structure, connection structure be the quick connect port of pipeline link, fixed layer one side intercommunication connecting layer, the clearance between opposite side and the connecting layer is the anchor clamps layer, connecting line include single way pipeline or double-circuit pipeline.
2. A connection device for perfusion in animal experiments according to claim 1, wherein: the utility model discloses a single way pipeline with double-circuit pipeline one side be the quick-connect male joint, be equipped with the screw thread with infusion tube connection one side outside, the nut inboard is equipped with the screw thread, the pipeline link outside is equipped with the screw thread and the nut inboard is equipped with the screw thread structure and matches each other.
3. A connection device for perfusion in animal experiments according to claim 1, wherein: the connecting layer, the clamp layer, the fixing layer and the connecting pipeline are all made of stainless steel or high polymer materials.
4. A connection device for perfusion in animal experiments according to claim 1, wherein: the front section of the clamp layer is provided with four separated elastic pieces, the four elastic pieces form a certain angle with the connecting layer, and the rear parts of the four elastic pieces are fixedly connected by round tubes; the front part of the fixing layer is unthreaded, the rear part is connected with the connecting layer through threads, and the surface of the fixing layer is provided with anti-skid threads.
5. A connection device for perfusion in animal experiments according to claim 1, wherein: the diameter of the port of the tissue connecting end is lower than the pipe diameter of the infusion pipeline, the port of the pipeline connecting end is a quick-connection female head, and the port of the pipeline connecting end can be connected with a single-way pipeline or a double-way pipeline.
6. A connection device for perfusion in animal experiments according to claim 1, wherein: the infusion pipeline and the connecting pipeline are sterilized by alcohol soaking and high-temperature high-pressure modes.
7. A connection device for perfusion in animal experiments according to claim 1, wherein: the single-way pipeline comprises a single-way connector and a single-way pipe orifice.
8. A connection device for perfusion in animal experiments according to claim 1, wherein: the two-way pipeline comprises a two-way connector, a first pipe orifice and a second pipe orifice.
9. The connection device for perfusion in animal experiments of claim 4, wherein: four shrapnel at the front section of the clamp layer can be opened or closed, and the aorta is fixed by the four shrapnels.
10. A connection device for perfusion in animal experiments according to claim 1, wherein: the peristaltic pump is arranged on the infusion tube and provides power for the solution in the tube by extruding the infusion tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321036683.7U CN220424015U (en) | 2023-05-04 | 2023-05-04 | Connecting device for perfusion in animal experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321036683.7U CN220424015U (en) | 2023-05-04 | 2023-05-04 | Connecting device for perfusion in animal experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220424015U true CN220424015U (en) | 2024-02-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321036683.7U Active CN220424015U (en) | 2023-05-04 | 2023-05-04 | Connecting device for perfusion in animal experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220424015U (en) |
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2023
- 2023-05-04 CN CN202321036683.7U patent/CN220424015U/en active Active
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