CN205099682U - Cud liquid does not have air supply product gas outward and simply installs - Google Patents
Cud liquid does not have air supply product gas outward and simply installs Download PDFInfo
- Publication number
- CN205099682U CN205099682U CN201520838055.XU CN201520838055U CN205099682U CN 205099682 U CN205099682 U CN 205099682U CN 201520838055 U CN201520838055 U CN 201520838055U CN 205099682 U CN205099682 U CN 205099682U
- Authority
- CN
- China
- Prior art keywords
- syringe
- ground
- triangular flask
- gas
- support body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cud liquid does not have the external product gas of air supply and simply installs, including vibration constant -temperature incubator, ground triangular flask and syringe, the ground triangular flask sets up in vibration constant -temperature incubator, the ground triangular flask is provided with the ground bottle plug, and the ground bottle plug is connected with coupling hose and keeps communicateing each other with the syringe mouth of syringe, and coupling hose is provided with the fixation clamp with the syringe mouth junction of syringe. The utility model discloses simple structure, low in cost, convenient to use, used ground triangular flask does not carry out aerogenic collection as the culture vessel, can realize effectively that the microorganism produces gaseous gas -liquid separation.
Description
Technical field
The utility model belongs to ruminant tumor gastric juice aerogenesis detection technique field, particularly relates to a kind of rumen fluid outer without source of the gas aerogenesis easy device.
Background technology
At present, easy rumen ectogenesis device mainly using syringe as fermentor tank, nutrient solution and fermentation substrate are put into the experimental study carrying out simulation rumen environment in syringe.But because syringe is very little, the gas of nutrient solution, culture substrate, tunning and generation all concentrates in syringe, makes gas collecting very inconvenient, easily causes testing error.
Utility model content
It is outer without source of the gas aerogenesis easy device that the object of the utility model embodiment is to provide a kind of rumen fluid, is intended to solve the culture apparatus gas collecting inconvenience used of rumen fluid gas production in vitro in prior art, easily causes the problem of testing error.
For achieving the above object, the technical solution adopted in the utility model is as follows:
A kind of rumen fluid is outer without source of the gas aerogenesis easy device, comprise vibration constant incubator, ground triangular flask and syringe, described ground triangular flask is arranged in vibration constant incubator, described ground triangular flask is provided with ground bottle stopper, described ground bottle stopper is connected with connecting hose and keeps being interconnected with the syringe port of syringe, and the syringe port junction of described connecting hose and syringe is provided with retaining clip.
Feature of the present utility model is also, vibration constant incubator is the difunctional gas bath constant temperature oscillator of ZD-85 type that Sheng Lan instrument manufacturing company limited of Jintan City produces.
Feature of the present utility model is also, the outer setting of syringe has scale.
Feature of the present utility model is also, syringe used is 100ml glass syringe, and ground triangular flask used is 100ml ground triangular flask, and ground bottle stopper used is the ground glass stopper that two ends communicate.
Feature of the present utility model is also, this rumen fluid is outer also comprises syringe laying rack without source of the gas aerogenesis easy device, syringe laying rack is made up of two the first support bodys mutually slided and the second support body, first support body is provided with slip ring, first support body and the second support body are slidably connected by slip ring, wherein be fixed with wave support above the first support body, the second support body be provided with corrugated anti-skidding hurdle.
The beneficial effects of the utility model are as follows:
The rumen fluid that the utility model provides is outer without source of the gas aerogenesis easy device, can solve the culture apparatus gas collecting inconvenience used of rumen fluid gas production in vitro in prior art, easily cause the problem of testing error; The utility model structure is simple, cheap, easy to use, ground triangular flask used, as culture vessel, does not carry out the collection of aerogenesis, effectively can carry out the gas-liquid separation of the angry body of production by biological, and ground triangular flask used is when carrying out fermentable, by the effective process to piston, in triangular flask, institute's aerogenesis body pushes syringe piston, effectively overcomes sliding resistance; Measure the relation between syringe unit volume and piston sliding distance with vernier calliper in advance, make reading more accurate, the spilling of produced gas in use can be ensured simultaneously.
Accompanying drawing explanation
Fig. 1 is the outer structural representation without source of the gas aerogenesis easy device of a kind of rumen fluid that the utility model embodiment provides;
In figure: 1, vibrate constant incubator; 2, ground triangular flask; 3, syringe; 4, ground bottle stopper; 5, connecting hose, 6, retaining clip, 7, syringe laying rack, 7-1, the first support body, 7-2, the second support body, 7-3, wave support, 7-4, anti-skidding hurdle, 7-5, slip ring.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with embodiment, the utility model is further elaborated.
As shown in Figure 1, a kind of rumen fluid of the utility model embodiment is outer without source of the gas aerogenesis easy device, comprise vibration constant incubator 1, ground triangular flask 2 and syringe 3, the quantity of ground triangular flask 2 and syringe 3 is consistent, ground triangular flask 2 is arranged in vibration constant incubator 1, ground triangular flask 2 is provided with ground bottle stopper 4, the ground glass stopper that ground bottle stopper 4 used communicates for two ends.Ground triangular flask 2 can be made to keep sealing with the junction of ground bottle stopper 4 by the setting of ground bottle stopper 4, ground bottle stopper 4 is connected with connecting hose 5 and keeps being interconnected with the syringe port of syringe, connecting hose 5 can be rubber hose, one end that rubber hose 5 is being connected with ground bottle stopper 4 is provided with connecting joint, the external diameter of connecting joint be greater than ground bottle stopper 4 with circular hole, so just can make rubber hose 5 quick and fastening insert in ground bottle stopper 4, realize fast, reliable connection, ground triangular flask 2 is connected with the injection port of syringe 3 by connecting hose 5, syringe 3 is positioned in vibration constant incubator 1 according to horizontal mode, connecting hose 5 is provided with retaining clip 6 with the injection port junction of syringe 3, ensure that connecting hose 5 is unlikely to the injection port landing from syringe 3.Vibration constant incubator 1 is the difunctional gas bath constant temperature oscillator of ZD-85 type that Sheng Lan instrument manufacturing company limited of Jintan City produces.Syringe 3 used is 100ml glass syringe, and the outer setting of syringe has scale.Ground triangular flask 2 used is 100ml ground triangular flask.
The utility model adopts ground triangular flask 2 as culture vessel, cud nutrient solution and the culture substrate of preparation is filled before test, and in syringe, suck 40ml damping fluid, for filling up the space stayed after exhausted air in connecting hose, triangular flask space, flexible pipe and syringe interface is again disconnected after air suction in triangular flask enters syringe, discharge outside air completely, again connect.This device is when carrying out fermentable, and nutrient solution upper volume is trained gradually and added, and can strengthen the strength that gas pushes syringe 3 piston, measures the relation between syringe unit volume and piston sliding distance simultaneously in advance, make reading more accurate with vernier calliper.
Consider that the utility model in use, syringe can be neat, stable and level be emitted in vibration constant incubator 1, as an optimal way of the present utility model, the rumen fluid of the utility model embodiment is outer also comprises syringe laying rack 7 without source of the gas aerogenesis easy device, syringe laying rack 7 used is made up of two the first support body 7-1 mutually slided and the second support body 7-2, first support body 7-1 is provided with slip ring 7-5, first support body 7-1 and the second support body 7-2 is slidably connected by slip ring 7-5, wherein be fixed with wave support 7-3 above the first support body 7-1, second support body 7-2 is provided with corrugated anti-skidding hurdle 7-4, anti-skidding hurdle 7-4 is made by rubber, play anti-skidding effect, in use, injector swing is placed on the top of the first support body 7-1 and the second support body 7-2, specifically, injector swing is placed on wave support 7-3 and anti-skidding hurdle 7-4, the rational height of wave support 7-3 and anti-skidding hurdle 7-4 meets after placement syringe, syringe can keep level.So can handled easily person fetch and the safety of in test syringe is put, can make the appropriate adjustments according to the size of syringe, and the reading on syringe also can clearly be observed, reading is convenient simultaneously.
The rumen fluid of the utility model embodiment outer without source of the gas aerogenesis easy device when simulating vitro culture use, first in each ground triangular flask 2, cud nutrient solution and culture substrate is added, then connecting hose 5 is utilized to be connected successively with syringe 3 (extracting 40ml damping fluid in syringe) by ground triangular flask 2 respectively, after connecting, twitch syringe 3, air in ground triangular flask 2 is extracted in syringe completely, then the junction of syringe 3 and connecting hose 5 is pushed aside, drain air, again connect connecting hose 5, by retaining clip 6, connecting hose 5 and syringe 3 are connected and fixed, then ground triangular flask 2 is placed in vibration constant incubator 1, then syringe laying rack 7 is positioned in vibration constant incubator 1, neat for syringe 3 is lain in a horizontal plane on syringe laying rack 7, connecting hose 5 is also placed in vibration constant incubator 1, under suitable temperature condition, in ground triangular flask 2, rumen microorganism produces a large amount of gas to substrate cultivation fermentation, gas is entered in syringe 3 by connecting hose 5.When cultivate certain hour or gas production rate reach syringe 3 range or 2/3 scale time, record gas production rate, and carry out the collection of aerogenesis.
The beneficial effects of the utility model are as follows:
The rumen fluid that the utility model provides is outer without source of the gas aerogenesis easy device, can solve the culture apparatus gas collecting inconvenience used of rumen fluid gas production in vitro in prior art, easily cause the problem of testing error; The utility model structure is simple, cheap, easy to use, ground triangular flask used, as culture vessel, does not carry out the collection of aerogenesis, effectively can realize the separation and the collection that produce gas, and ground triangular flask used is when carrying out fermentable, by the effective process (as smearing Vaseline) to piston, in triangular flask, institute's aerogenesis body pushes syringe piston, effectively overcomes sliding resistance; Measure the relation between syringe unit volume and piston sliding distance with vernier calliper in advance, make reading more accurate, the spilling of produced gas in use can be ensured simultaneously; The utility model in use, can carry out the cultivation of multiple sample simultaneously, substantially increase culture efficiency.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.
Claims (5)
1. a rumen fluid is outer without source of the gas aerogenesis easy device, it is characterized in that, comprise vibration constant incubator, ground triangular flask and syringe, described ground triangular flask is arranged in vibration constant incubator, described ground triangular flask is provided with ground bottle stopper, described ground bottle stopper is connected with connecting hose and keeps being interconnected with the syringe port of syringe, and the syringe port junction of described connecting hose and syringe is provided with retaining clip.
2. a kind of rumen fluid according to claim 1 is outer is characterized in that without source of the gas aerogenesis easy device, and described vibration constant incubator is the difunctional gas bath constant temperature oscillator of ZD-85 type that Sheng Lan instrument manufacturing company limited of Jintan City produces.
3. a kind of rumen fluid according to claim 1 is outer without source of the gas aerogenesis easy device, and it is characterized in that, the outer setting of described syringe has scale.
4. a kind of rumen fluid according to claim 1 is outer without source of the gas aerogenesis easy device, it is characterized in that, syringe used is 100ml glass syringe, and ground triangular flask used is 100ml ground triangular flask, and ground bottle stopper used is the ground glass stopper that two ends communicate.
5. a kind of rumen fluid according to claim 1 is outer without source of the gas aerogenesis easy device, it is characterized in that, described rumen fluid gas production in vitro easy device also comprises syringe laying rack, syringe laying rack is made up of two the first support bodys mutually slided and the second support body, first support body is provided with slip ring, first support body and the second support body are slidably connected by slip ring, are wherein fixed with wave support above the first support body, the second support body are provided with corrugated anti-skidding hurdle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520838055.XU CN205099682U (en) | 2015-10-20 | 2015-10-20 | Cud liquid does not have air supply product gas outward and simply installs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520838055.XU CN205099682U (en) | 2015-10-20 | 2015-10-20 | Cud liquid does not have air supply product gas outward and simply installs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205099682U true CN205099682U (en) | 2016-03-23 |
Family
ID=55515472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520838055.XU Expired - Fee Related CN205099682U (en) | 2015-10-20 | 2015-10-20 | Cud liquid does not have air supply product gas outward and simply installs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205099682U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108261602A (en) * | 2018-01-03 | 2018-07-10 | 山东师范大学 | A kind of array glass-micropipe device for casting and preparation method thereof |
-
2015
- 2015-10-20 CN CN201520838055.XU patent/CN205099682U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108261602A (en) * | 2018-01-03 | 2018-07-10 | 山东师范大学 | A kind of array glass-micropipe device for casting and preparation method thereof |
| CN108261602B (en) * | 2018-01-03 | 2020-11-20 | 山东师范大学 | Array type glass micro-tube filling device and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203224384U (en) | Special soil percolate extracting device for rice field | |
| CN202099277U (en) | Simulated rumen in vitro fermentation gas producing device | |
| CN205099682U (en) | Cud liquid does not have air supply product gas outward and simply installs | |
| CN202152343U (en) | Artificial rumen in-vitro fermentation gas-producing device | |
| CN101480135A (en) | Device for cultivating liquid culture of edible fungus | |
| CN204581307U (en) | A kind of vacuum test tube | |
| CN204138670U (en) | A kind of cell culture bags and support thereof | |
| CN204918609U (en) | Cell culture bag that volume changes | |
| CN204373961U (en) | Do not topple over disposable urine cup | |
| CN203535039U (en) | Electrical logging soil depressimeter | |
| CN208442571U (en) | A kind of gas negative pressure collection device | |
| CN202854113U (en) | Biological crust gas reaction container | |
| CN209677035U (en) | One kind being suitable for plant rhizosphere13CO2The culture apparatus of gas label | |
| CN203159597U (en) | Artificial simple rumen in-vitro fermentation gas production device | |
| CN206706123U (en) | A kind of cell culture bags | |
| CN202875842U (en) | Nasal feeding tube | |
| CN207143263U (en) | A kind of novel cell culture bag | |
| CN205313577U (en) | Aseptic cell culture bottle | |
| CN204779580U (en) | Simple and easy artificial rumen blake bottle | |
| CN201208261Y (en) | Hemostix | |
| CN202724323U (en) | Drainage device for emergency internal medicine | |
| CN205164767U (en) | Take burette support of sucking disc | |
| CN203465114U (en) | Sediment interstitial water collection device for laboratory | |
| CN201168267Y (en) | Looped link of injection moulding infusion bottle | |
| CN207418765U (en) | Portable suspension cell culture and chemicals dosing plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160323 Termination date: 20161020 |