CN205473714U - Microorganism non -contact liquid culture apparatus - Google Patents
Microorganism non -contact liquid culture apparatus Download PDFInfo
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- CN205473714U CN205473714U CN201620150883.9U CN201620150883U CN205473714U CN 205473714 U CN205473714 U CN 205473714U CN 201620150883 U CN201620150883 U CN 201620150883U CN 205473714 U CN205473714 U CN 205473714U
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- culture device
- contactless liquid
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Abstract
The utility model discloses a microorganism non -contact liquid culture apparatus, including the culture vessel, the culture vessel hangs built -in upper and lower both ends open -ended drum, and the drum lower extreme seals fixed pellicle. The utility model discloses the non -contact that can be used to between two or the two types of bacterials co -cultures, and research is altogether under the culture conditions, the influence of micro molecule metabolite to co -culturing the bacterial strain. The device's advantage lies in split type design, realizes easily that the non -contact liquid of two kinds or two bacteroids co -cultures, on can arranging the shaking table in, make things convenient for culture medium make full use of, easily at cultivation section collection any time in period or recovery culture medium and thallus, the pellicle be liable to change who prevents two kinds of bacterial contacts simultaneously, the device reuse rate height, easy operation, the suitable popularization and application.
Description
Technical field
This utility model belongs to microbial co culture device field, is specifically related to a kind of microorganism contactless liquid co-culture device.
Background technology
At present microorganism subject have numerous studies directly to contact around different strain, during non-direct contact, the impact on growth metabolism situation each other.Wherein, directly contact can realize by being placed in by strain in same culture medium, relatively easy；And for non-direct contact, traditional method is that one strain is applied to solid culture primary surface, one layer of semipermeable membrane of upper covering, then another strain is applied on filter membrane；This kind of method is only used for solid medium and cultivates, and cannot calculate cell concentration during co-culturing, and monitoring in real time co-cultures situation and acquires a certain degree of difficulty.
Summary of the invention
It is an object of the invention to provide a kind of microorganism contactless liquid co-culture device, this device can realize the liquid noncontact of microorganism and co-culture, and its simple in construction, easy to operate, is suitable for popularization and application.
For achieving the above object, the technical solution used in the present invention is:
A kind of microorganism contactless liquid co-culture device, including culture vessel, described culture vessel hangs the cylinder of built-in upper/lower terminal opening, and cylinder lower end closed fixes semipermeable membrane.
Preferably, described cylinder lower end arranges external screw thread.
Preferably, described semipermeable membrane is fixed on foundation ring, and foundation ring is in the form of annular discs, and foundation ring size matches with cylinder outer wall, and inwall bottom, foundation ring arranges female thread, and the external screw thread that female thread is arranged with cylinder lower end matches；Bottom, foundation ring arranges lower annulus, and semipermeable membrane is fixed on lower annulus；Cylinder and foundation ring realize being tightly connected by internal and external screw thread, and threadeding by foundation ring and cylinder lower end, the conveniently replaced semipermeable membrane hindering antibacterial to contact.
Preferably, described cylinder bottom arranges and is connected fixing upper annulus with cylinder inner wall, is closely pressed on the semipermeable membrane on lower annulus when it carries out threaded by internal and external screw thread, it is achieved fixing of semipermeable membrane.
The distance of described cylinder lower end distance culture vessel inner bottom surface shows that condition of culture is adjusted, and makes culture fluid that semipermeable membrane is immersed in culture vessel and culture fluid be difficult to overflow.
The described cylinder lower end distance away from culture vessel inner bottom surface is 0.5～2cm.
Preferably, the described cylinder lower end distance away from culture vessel inner bottom surface is 0.5～1cm.
Described culture vessel is symmetrical arranged support arm along bottleneck, in card channel-shaped.
Described cylinder upper end is symmetrical arranged support arm, and its size matches with support.
Described culture vessel is triangular flask, conical flask or beaker-type shape.
Described semipermeable membrane can be selected for composite fibre resin filter membrane, louver film, nitrocellulose filter, cellulose acetate sheets or nylon leaching film etc..
Described semipermeable membrane pore diameter range is 0.15～5.0 μm, can realize passing through of culture fluid, and hinder different strain to contact.
Described culture vessel can be selected for glass, lucite material etc..
Described cylinder can be selected for polypropylene material etc..
Described upper annulus and lower annulus can be selected for politef etc..
This utility model, on the premise of ensureing that nutrient substance and secondary metabolite pass freely through, can realize physical barrier strict between different microorganisms thalline, reach the purpose that noncontact between microorganisms co-cultures；And cultivation can be placed directly within shaking table, it is achieved during microbial liquid is cultivated, observe the purpose that noncontact co-cultures；Facilitate the later stage to extract the metabolite in culture fluid, and two kinds of respective upgrowth situations of strain in independent studies co-culture system simultaneously.
The invention have the benefit that
The contactless liquid that can be used between two strains or two class strains co-cultures；And use can be directly placed on shaking table, facilitate culture medium to make full use of, and the extraction of later stage metabolite；The semipermeable membrane simultaneously stoping strain contact is convenient for changing, and device recycling rate of waterused is high, easy to use.
Accompanying drawing explanation
It is the structural representation of one embodiment of this utility model below:
Fig. 1 is the structural representation of microorganism contactless liquid co-culture device.
Fig. 2 is the structural representation of culture vessel.
Fig. 3 is the structural representation of cylinder.
Fig. 4 is the structural representation of foundation ring.
Wherein: 1, culture vessel, 2, cylinder, 3, semipermeable membrane, 4, foundation ring, 5, support, 6, support arm, 7, upper annulus, 8, external screw thread, 9, female thread, 10, lower annulus.
Detailed description of the invention
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with drawings and Examples, it is further elaborated to this use is novel.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.As long as just can be mutually combined additionally, technical characteristic involved in each embodiment of invention described below does not constitutes conflict each other.
As depicted in figs. 1 and 2, a kind of microorganism contactless liquid co-culture device described in the utility model, including culture vessel 1 (in triangular flask shape, lucite material), culture vessel 1 bottleneck is symmetrical arranged two supports 5, and support 5 is in card channel-shaped, and cylinder 2 (polypropylene material) is hung by support 5 and is built in culture vessel 1, the upper/lower terminal opening of described cylinder 2, fixing semipermeable membrane 3 (composite fibre resin filter membrane) is closed by foundation ring 4 in cylinder 2 lower end.
As it is shown on figure 3, described cylinder 2 upper end is symmetrical arranged two support arms 6, support arm 6 size matches with the draw-in groove of support 5, makes cylinder 2 can be suspended in culture vessel 1；Cylinder 2 lower end arranges external screw thread 8, and cylinder 2 bottom arranges and is connected fixing upper annulus 7 (politef material) with cylinder 2 inwall.
As shown in Figure 4, described foundation ring 4 is in the form of annular discs, and foundation ring 4 size matches with cylinder 2, and its inwall arranges female thread 9, and the external screw thread 8 that female thread 9 is arranged with cylinder 2 lower end matches；Bottom, foundation ring 4 arranges lower annulus 10, is used for fixing and supporting semipermeable membrane 3, and semipermeable membrane 3 is placed on lower annulus 10；When cylinder and foundation ring are carried out threaded (female thread 9, external screw thread 8), annulus 7 is made closely to be pressed on the semipermeable membrane 3 on lower annulus 10, it is achieved fixing of semipermeable membrane 3；And conveniently replaced hinder antibacterial contact semipermeable membrane 3.
Described microorganism contactless liquid co-culture device in use, first in the environment of sterile working in, by culture fluid fall in culture vessel, inoculate strain A；Helicitic texture is passed through in the foundation ring placing semipermeable membrane and is engaged sealing fixing semipermeable membrane with cylinder, the cylinder assembled and foundation ring structure are hung in culture vessel by support arm, can be directly toward pouring culture fluid in cylinder into or the culture fluid for the treatment of in culture vessel slow transits through semipermeable membrane and enters in cylinder (in culture vessel, the liquid level of culture fluid should be higher than that the level height of semipermeable membrane), strain B is inoculated in cylinder, cover bottle cap, then can quiescent culture or entirety is put in shaking table cultivate；During experiment, bacterium solution can be drawn at any time and observe growth situation, count, extract metabolite etc..
Those skilled in the art is easy to understand; the foregoing is only presently preferred embodiments of the present invention; not in order to limit the present invention, all any amendment, equivalent and improvement etc. made within the spirit and principles in the present invention, should be included within the scope of the present invention.
Claims (9)
1. a microorganism contactless liquid co-culture device, including culture vessel, it is characterised in that in described culture vessel hangs
Putting the cylinder of upper/lower terminal opening, cylinder lower end closed fixes semipermeable membrane.
Microorganism contactless liquid co-culture device the most according to claim 1, it is characterised in that described cylinder lower end is arranged
External screw thread.
Microorganism contactless liquid co-culture device the most according to claim 2, it is characterised in that described semipermeable membrane is fixed on
On foundation ring, foundation ring is in the form of annular discs, and foundation ring size matches with cylinder outer wall, and inwall bottom, foundation ring arranges female thread, female thread
The external screw thread arranged with cylinder lower end matches；Bottom, foundation ring arranges lower annulus, and semipermeable membrane is fixed on lower annulus；Cylinder and the end
Enclose and realize being tightly connected by internal and external screw thread.
Microorganism contactless liquid co-culture device the most according to claim 3, it is characterised in that described cylinder bottom is arranged
Be connected fixing upper annulus with cylinder inner wall, be closely pressed in when it carries out threaded by internal and external screw thread on lower annulus is semi-transparent
On film, it is achieved fixing of semipermeable membrane.
Microorganism contactless liquid co-culture device the most according to claim 1, it is characterised in that described culture vessel is along bottle
Mouth is symmetrical arranged support, in card channel-shaped.
Microorganism contactless liquid co-culture device the most according to claim 5, it is characterised in that described cylinder upper end is symmetrical
Arranging support arm, its size matches with support.
Microorganism contactless liquid co-culture device the most according to claim 1, it is characterised in that described culture vessel is three
Angle bottle, conical flask or beaker-type shape.
Microorganism contactless liquid co-culture device the most according to claim 1, it is characterised in that described semipermeable membrane is mixing
Fibre resin filter membrane, louver film, nitrocellulose filter, cellulose acetate sheets or nylon leaching film.
Microorganism contactless liquid co-culture device the most according to claim 1, it is characterised in that described culture vessel uses
Lucite material；Cylinder uses polypropylene material；Upper annulus and lower annulus use politef material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620150883.9U CN205473714U (en) | 2016-02-29 | 2016-02-29 | Microorganism non -contact liquid culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620150883.9U CN205473714U (en) | 2016-02-29 | 2016-02-29 | Microorganism non -contact liquid culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205473714U true CN205473714U (en) | 2016-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620150883.9U Expired - Fee Related CN205473714U (en) | 2016-02-29 | 2016-02-29 | Microorganism non -contact liquid culture apparatus |
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| CN (1) | CN205473714U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107794223A (en) * | 2017-11-07 | 2018-03-13 | 广东药科大学附属第医院 | Anaerobic bacteria and the in vitro study model and method of aerobic cell interaction in cell co-culture device and analogue body |
| CN110616140A (en) * | 2019-10-28 | 2019-12-27 | 上海国睿生命科技有限公司 | Cell co-culture dish |
| CN111139174A (en) * | 2020-01-15 | 2020-05-12 | 辽东学院 | Mixed microorganism culture device |
| CN111398086A (en) * | 2020-04-02 | 2020-07-10 | 台州龙江化工机械科技有限公司 | Liquid ammonia water content measuring device and measuring method thereof |
| CN112852616A (en) * | 2021-01-22 | 2021-05-28 | 福建省微生物研究所 | Fluid bioreactor and use method thereof |
-
2016
- 2016-02-29 CN CN201620150883.9U patent/CN205473714U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107794223A (en) * | 2017-11-07 | 2018-03-13 | 广东药科大学附属第医院 | Anaerobic bacteria and the in vitro study model and method of aerobic cell interaction in cell co-culture device and analogue body |
| CN110616140A (en) * | 2019-10-28 | 2019-12-27 | 上海国睿生命科技有限公司 | Cell co-culture dish |
| CN111139174A (en) * | 2020-01-15 | 2020-05-12 | 辽东学院 | Mixed microorganism culture device |
| CN111139174B (en) * | 2020-01-15 | 2023-08-29 | 辽东学院 | Mixed microorganism culture apparatus |
| CN111398086A (en) * | 2020-04-02 | 2020-07-10 | 台州龙江化工机械科技有限公司 | Liquid ammonia water content measuring device and measuring method thereof |
| CN112852616A (en) * | 2021-01-22 | 2021-05-28 | 福建省微生物研究所 | Fluid bioreactor and use method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160817 Termination date: 20190228 |