CN113969237A - Totally closed formula microorganism inoculation marking off gathers and cultivates external member - Google Patents
Totally closed formula microorganism inoculation marking off gathers and cultivates external member Download PDFInfo
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- CN113969237A CN113969237A CN202111213292.3A CN202111213292A CN113969237A CN 113969237 A CN113969237 A CN 113969237A CN 202111213292 A CN202111213292 A CN 202111213292A CN 113969237 A CN113969237 A CN 113969237A
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- 238000011081 inoculation Methods 0.000 title claims abstract description 35
- 244000005700 microbiome Species 0.000 title claims abstract description 33
- 238000003860 storage Methods 0.000 claims abstract description 39
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 37
- 230000006835 compression Effects 0.000 claims abstract description 24
- 238000007906 compression Methods 0.000 claims abstract description 24
- 238000012258 culturing Methods 0.000 claims abstract description 15
- 238000002347 injection Methods 0.000 claims description 20
- 239000007924 injection Substances 0.000 claims description 20
- 238000007789 sealing Methods 0.000 claims description 17
- 238000009434 installation Methods 0.000 claims description 15
- 230000000813 microbial effect Effects 0.000 claims description 10
- 238000003306 harvesting Methods 0.000 claims description 8
- 238000005192 partition Methods 0.000 claims description 7
- 238000001179 sorption measurement Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 230000000717 retained effect Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 101100441413 Caenorhabditis elegans cup-15 gene Proteins 0.000 description 22
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 230000006872 improvement Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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Abstract
The application belongs to the technical field of microorganism collecting, culturing and lineation matched equipment, and particularly relates to a totally-enclosed microorganism inoculation lineation collecting and culturing suite. Comprises a collecting component, a culturing component and a magnetic marking head; the collection assembly includes: the collecting cup, the pressure-bearing part, the first compression spring, the sliding part, the storage cup and the reagent kit are sequentially arranged from top to bottom; the culture assembly comprises a culture box and a box cover; the box cover is detachably buckled on the culture box, and a closed culture cavity is formed inside the box cover; the magnetic scribing head includes: the inoculation needle is sleeved at the lower end of the supporting cylinder and can slide up and down; a magnet embedded in the upper end of the support cylinder; a second compression spring with two ends abutting against the magnet and the inoculating needle. The utility model provides a totally closed formula microorganism inoculation marking off gathers and cultivates external member, overall structure is compact and small and exquisite, convenient to use, low in production cost, each accessory can be alone, the combination uses, each part both keeps the function independent, has and can make up the use fast and accomplish some operation contents.
Description
Technical Field
The application belongs to the technical field of microorganism collecting, culturing and lineation matched equipment, and particularly relates to a totally-enclosed microorganism inoculation lineation collecting and culturing suite.
Background
In the process of culturing and detecting microorganisms, a microorganism sample needs to be collected by using special collecting equipment, transferred from the collecting equipment to culturing equipment by using a scribing tool, and subjected to enrichment culture on a culture medium by scribing so as to obtain a bacterial colony meeting the detection requirement. Because the above-mentioned flow involves multiple container tool and multichannel transfer procedure, on the one hand the operation is very loaded down with trivial details inefficiency, on the other hand opens many times and shifts and also can lead to the microorganism sample frequently to expose in external environment, leads to the invasion of external pollution source easily to lead to the cultivation result to not conform to the requirements, because the sample is tiny be difficult for noticing, consequently can lead to the useless consumption of a large amount of working costs.
Disclosure of Invention
The present application is directed to provide a totally enclosed microorganism inoculation and streaking harvesting and culturing kit for accomplishing operations such as microorganism collection, streaking, and culturing in a totally enclosed environment, based on the above-mentioned current situation.
In order to achieve the purpose, the following technical scheme is adopted in the application.
The totally-enclosed microorganism inoculation streak collection culture kit comprises a collection assembly 1, a culture assembly 2 and a magnetic streak head 3;
the collecting assembly 1 comprises: the collecting cup 11, the pressure bearing part 12, the first compression spring 13, the sliding part 14, the storage cup 15 and the reagent kit 16 are arranged from top to bottom in sequence;
the reagent cartridge 16 includes a cartridge body portion 160 provided with the mounting chamber 16a, and a hollow connecting portion 161 provided on one side of the mounting chamber 16 a;
a flow guide hole 16b communicated with the connecting part 161 is formed in the inner wall of the mounting cavity 16 a; the inner cavity of the connecting part 161 is communicated with the mounting cavity 16a through the diversion hole 16 b;
a guide outlet is formed in the outer side of the connecting portion 161, a first baffle 162 is installed at the guide outlet, and the first baffle 162 blocks the guide outlet through a rotating shaft and a torsion spring;
the shape of the storage cup 15 is matched with that of the installation cavity 16a, the upper end of the storage cup 15 is opened, the center of the bottom of the storage cup 15 is provided with an injection hole 15a, a connecting cylinder 15b is arranged outside the injection hole 15a, the cylinder wall of the connecting cylinder 15b is provided with a plurality of connecting holes 15c, and the cylinder wall of the connecting cylinder 15b is provided with a drainage hole or a drainage groove 15d connected to the cavity bottom of the installation cavity 16 a;
the sliding piece 14 is arranged in the connecting cylinder 15b in a vertically sliding manner and comprises a sealing part 14a with the middle part capable of abutting against and sealing the injection hole 15a, and a pressing part 14b capable of penetrating out of the injection hole 15a is arranged on the lower side of the sealing part 14 a;
the upper end of the pressure-bearing part 12 is propped against the collecting cup 11, and the upper end of the first compression spring 13 is propped against the lower end of the pressure-bearing part 12 and is propped against the sliding part 14; the outer side of the pressure bearing piece 12 is provided with a convex part 12a which can be buckled in the connecting hole 15 c;
the collecting cup 11 is buckled at the opening of the reagent box 16, the cup bottom of the collecting cup 11 is propped against the pressure-bearing part 12, and the sealing part 14a is pressed against the injection hole 15a after the first compression spring 13 is compressed;
the culture assembly 2 is close to the connecting part 161 and is arranged at one side of the outlet;
the culture assembly 2 comprises a culture box 20 and a box cover 21; the box cover 21 is detachably buckled on the culture box 20, and a closed culture cavity 201 is formed inside the box cover;
a leading-in channel 202 is arranged on one side of the culture cavity 201 facing the connecting part 161, the leading-in channel 202 is opposite to the leading-out port, a second baffle 203 is arranged at the outlet of the leading-in channel, and the second baffle 203 plugs the leading-in channel 202 through a rotating shaft and a torsion spring;
the magnetic scribing head 3 is arranged at the inner side of the connecting part 161 and close to the outlet;
the magnetic scribing head 3 includes: a supporting cylinder 30 and an inoculating needle 31 which is sleeved at the lower end of the supporting cylinder 30 and can slide up and down; a magnet 32 embedded in the upper end of the support cylinder 30; a second compression spring 33 having two ends bearing against the magnet 32 and the inoculating needle 31.
In a further improvement and preferred embodiment of the totally-enclosed microorganism inoculation, lineation, collection and culture kit, a step hole 30a is formed at the opening at the lower end of the supporting cylinder 30, and the size of the upper end of the inoculation needle 31 is larger than the aperture of the step hole 30a so as to be clamped on the step of the limiting step hole 30 a;
the upper end of the inoculating needle 31 is provided with a spring mounting groove 31a for accommodating the lower end of the second compression spring 33, and the lower end of the inoculating needle 31 is provided with an inoculating loop 31 b.
In a further improvement and preferred embodiment of the totally enclosed microorganism inoculation, lineation, collection and culture kit, the box body part 160 and the collection cup 11 are of a round bottle cap-shaped structure;
the upper edge of the box body part 160 and the lower edge of the collecting cup 11 are buckled with each other;
the inner side of the lower edge of the collecting cup 11 extends downwards to form a leakage-proof ring 111, and when the collecting cup 11 is buckled at the opening of the reagent box 16, the leakage-proof ring 111 extends into the inner cavity of the storage cup 15 and is close to the cavity wall of the storage cup 15;
the bottom of the connecting cylinder 15b is dug down to be lower than the bottom of the storage cup 15 so that when the slider 14 is moved upward, the liquid can flow from the bottom of the storage cup 15 to the filling hole 15a in the center of the connecting cylinder 15 b.
In a further improved and preferred embodiment of the fully enclosed microorganism inoculation streak collection and culture kit, a guide column 14c is arranged on the upper side of the sealing part 14 a; the pressure-bearing part 12 is annular and is sleeved on the guide post 14 c;
the first compression spring 13 is fitted over the guide post 14 c.
In a further improved and preferred embodiment of the fully enclosed microbial inoculation streaking harvesting and culturing assembly described above, the collection cup 11 includes a top receptacle portion 110; the collecting cup 11 is provided in plurality, and each collecting cup 11 has a receiving portion 110 having a different volume.
In a further improved and preferred embodiment of the fully enclosed microorganism inoculation, streaking, collecting and culturing assembly, the culture box 20 is a shell structure with an open upper side;
a culture tank 20a for containing a culture medium is provided in the middle of the culture box 20, and the lower half of the introduction passage 202 is formed by a tank structure communicated to the culture tank 20 a;
the rear side of the culture tank 20a is provided with an object placing tank 20b, and the bottom of the object placing tank 20b is provided with a metal block or a magnetic block 208 which can attract the magnet 32.
In a further improved and preferred embodiment of the fully enclosed microorganism inoculation and streaking collection and culture kit, the reagent kit 16 is provided with a plurality of connecting portions 161;
the hollow part of each connecting part 161 is internally provided with mutually independent contact cavities 163, the bottoms of the contact cavities 163 are respectively communicated with the mounting cavity 16a through the diversion holes 16b, and the bottom of each contact cavity 163 is lower than the bottom of the mounting cavity 16 a; magnetic scribe head 3 is disposed within contact cavity 163 with inoculating needle 31 facing the bottom of contact cavity 163.
In a further improved and preferred embodiment of the fully enclosed microorganism inoculation and streaking collection and culture kit, a partition plate 164 is disposed inside the installation cavity 16 a;
the partition plate 164 is divided into an inner groove 165 corresponding to the injection hole 15a inside and an outer groove 166 outside in the mounting cavity 16 a; the inner groove 165 extends toward the guide hole 16b and communicates with the contact chamber 163.
In a further improved and preferred embodiment of the fully enclosed microorganism inoculation and streaking collection and culture kit, a reagent adsorption sponge is disposed in the outer trough 166, and when the collection cup 11 is retained in the reagent kit 16, the bottom of the reagent kit 16 is adjacent to the reagent adsorption sponge.
In a further improvement and preferred embodiment of the totally enclosed microorganism inoculation streak collection and culture kit, the inner grooves 165 connected with the connecting parts 161 are combined and communicated in the center of the installation cavity 16 a;
the bottom of the inner groove 165 connected to each connecting portion 161 is a flat inclined surface, and the side with the higher inclined surface faces the center of the mounting cavity 16 a.
The beneficial effects are that:
the totally-enclosed microorganism inoculation, lineation, collection and culture kit has the advantages of compact and small overall structure, convenience in use and low production cost, all accessories can be used independently and in combination, all parts are independent in function, and a series of operation contents can be completed by quick combination and use;
after the microorganisms are preliminarily collected, the sealing operation in the whole process of subsequent material transfer, reagent addition and streak culture can be ensured, the pollution invasion of the external environment is effectively avoided, and the accuracy of the culture test result is improved;
by matching design of all collecting and culturing accessories, the device is small and light in overall volume, and can be conveniently subjected to batch industrial application and automatic disposal;
by adopting the structure form of the built-in scribing head, the sample can be transferred and the outside magnetic adsorption structure can be utilized to carry out non-contact operation outside the culture box shell in the process of culturing and scribing, and the leakage of internal microorganisms can be effectively prevented.
Drawings
FIG. 1 is a schematic view (one-by-one) of a fully enclosed microbial inoculation streaking collection and culture kit;
FIG. 2 is an assembled schematic view of the collection assembly;
FIG. 3 is a schematic view of the assembly of the culture assembly;
FIG. 4 is a schematic diagram of the construction of a magnetic scribing head;
FIG. 5 is a schematic view of a fully enclosed microbial inoculation streaking harvesting culture kit (one for two);
FIG. 6 is a schematic structural view of a storage cup;
wherein the reference numerals include:
the collection unit 1, the collection cup 11, the accommodating part 110, the pressure receiving member 12, the first compression spring 13, the sliding member 14, the sealing part 14a, the pressing part 14b, the guide post 14c, the storage cup 15, the injection hole 15a, the connecting cylinder 15b, the connecting hole 15c, the drainage groove 15d, the reagent cartridge 16, the mounting cavity 16a, the drainage hole 16b, the cartridge body part 160, the connecting part 161, the first baffle 162, the contact cavity 163, the partition plate 164, the inner groove 165, the outer groove 166, the culture unit 2, the culture cartridge 20, the culture tank 20a, the storage groove 20b, the cartridge cover 21, the culture chamber 201, the introduction channel 202, the second baffle 203, the magnet block 208, the magnetic scribing head 3, the support cylinder 30, the stepped hole 30a, the inoculating needle 31, the spring mounting groove 31a, the inoculating loop 31b, the magnet 32, and the second compression spring 33.
Detailed Description
The present application will be described in detail with reference to specific examples.
The utility model provides a totally closed formula microorganism inoculation marking off is gathered and is cultivateed external member, mainly used collects fast as the microorganism, reagent interpolation, the support carrier of marking off cultivation, and the main objective provides a compact structure is light small and exquisite, can realize in totally closed environment that the microorganism is collected, shifted and the marking off, is convenient for carry out automatic continuity of operation, realizes the operation in batches, is applicable to the novel collection cultivation external member of industry application.
The basic structure of the totally-enclosed microorganism inoculation lineation acquisition culture kit comprises a collection assembly 1, a culture assembly 2 and a magnetic lineation head 3;
as shown in FIGS. 1 and 5, the collection unit may be configured to transfer microorganisms to one or more culture units as needed to reduce the amount of labor, and it should be noted that a separate magnetic scribing head is generally provided at the connection portion of the collection unit for each culture unit.
As shown in fig. 2 and 6, the collecting unit 1 includes: the collecting cup 11, the pressure bearing part 12, the first compression spring 13, the sliding part 14, the storage cup 15 and the reagent kit 16 are arranged from top to bottom in sequence;
the reagent cartridge 16 includes a cartridge body portion 160 provided with the mounting chamber 16a, and a hollow connecting portion 161 provided on one side of the mounting chamber 16 a; a flow guide hole 16b communicated with the connecting part 161 is formed in the inner wall of the mounting cavity 16 a;
the inner cavity of the connecting part 161 is communicated with the mounting cavity 16a through the diversion hole 16 b; a guide outlet is formed in the outer side of the connecting portion 161, a first baffle 162 is installed at the guide outlet, and the first baffle 162 blocks the guide outlet through a rotating shaft and a torsion spring;
the shape of the storage cup 15 is matched with that of the installation cavity 16a, the upper end of the storage cup 15 is opened, the center of the bottom of the storage cup 15 is provided with an injection hole 15a, a connecting cylinder 15b is arranged outside the injection hole 15a, the cylinder wall of the connecting cylinder 15b is provided with a plurality of connecting holes 15c, and the cylinder wall of the connecting cylinder 15b is provided with a drainage hole or a drainage groove 15d connected to the cavity bottom of the installation cavity 16 a;
the sliding piece 14 is arranged in the connecting cylinder 15b in a vertically sliding manner and comprises a sealing part 14a with the middle part capable of abutting against and sealing the injection hole 15a, and a pressing part 14b capable of penetrating out of the injection hole 15a is arranged on the lower side of the sealing part 14 a;
the upper end of the pressure-bearing part 12 is propped against the collecting cup 11, and the upper end of the first compression spring 13 is propped against the lower end of the pressure-bearing part 12 and is propped against the sliding part 14; the outer side of the pressure bearing piece 12 is provided with a convex part 12a which can be buckled in the connecting hole 15 c;
the collecting cup 11 is buckled at the opening of the reagent box 16, the cup bottom of the collecting cup 11 is propped against the pressure-bearing part 12, and the sealing part 14a is pressed against the injection hole 15a after the first compression spring 13 is compressed;
the culture assembly 2 is close to the connecting part 161 and is arranged at one side of the outlet;
the culture assembly 2 comprises a culture box 20 and a box cover 21; the box cover 21 is detachably buckled on the culture box 20, and a closed culture cavity 201 is formed inside the box cover;
a leading-in channel 202 is arranged on one side of the culture cavity 201 facing the connecting part 161, the leading-in channel 202 is opposite to the leading-out port, a second baffle 203 is arranged at the outlet of the leading-in channel, and the second baffle 203 plugs the leading-in channel 202 through a rotating shaft and a torsion spring;
the magnetic scribing head 3 is arranged at the inner side of the connecting part 161 and close to the outlet;
the magnetic scribing head 3 includes: a supporting cylinder 30 and an inoculating needle 31 which is sleeved at the lower end of the supporting cylinder 30 and can slide up and down;
a magnet 32 embedded in the upper end of the support cylinder 30; a second compression spring 33 having two ends bearing against the magnet 32 and the inoculating needle 31.
In the specific use process, the collecting cup 11 is taken down, the collecting cup 11 is used for collecting liquid microorganism samples, the whole mechanism formed by the pressure-bearing part 12, the first compression spring 13, the sliding part 14 and the storage cup 15 is buckled with the collecting cup 11 after collection to form a closed storage device, at the moment, the storage cup 15 is equivalent to the cup cover of the collecting cup 11, and the whole is used as a sealed storage container for storage or standing for later use;
when reagent adding and transfer culture are needed, the liquid sample is inverted, the liquid sample is transferred from the collecting cup 11 to the storage cup 15, the collecting cup 11 is equivalent to the cup cover of the storage cup 15, and the injection hole is blocked by the sliding piece to prevent leakage;
after the structure is inverted, the integral structure is arranged in an installation cavity on a reagent box 16 and connected with the reagent box, when a collection cup 11 is pressed, a pressure-bearing piece 12 and a storage cup 15 are pushed to move downwards, the lower end of a sliding piece 14 abuts against the bottom of the installation cavity 16a to move upwards, a filling hole is opened, and a sample flows downwards to the storage cup;
the storage cup is also used for storing a prepared reagent to be added, the sample flows to the inner cavity of the connecting part 161 along with the reagent, and the inoculation needle 31 at the lower end of the magnetic scribing head 3 is contacted with the sample to prepare for scribing and transferring;
in transferring and streaking, the collection module 1 and the incubation module 2 are butted, and the magnetic streaking head 3 is attracted and moved externally by a magnetic attracting device through the top wall of the reagent cartridge 16 or the lid 21 of the incubation cartridge, so that the sample on the inoculating needle 31 is transferred and streaked therewith.
In order to facilitate the use of the magnetic scribing head 3, obtain a sample conveniently and not damage a culture medium, a stepped hole 30a is formed at the opening at the lower end of the supporting cylinder 30, and the size of the upper end of the inoculating needle 31 is larger than the aperture of the stepped hole 30a so as to be clamped on the step of the limiting stepped hole 30 a; the upper end of the inoculating needle 31 is provided with a spring mounting groove 31a for accommodating the lower end of the second compression spring 33, and the lower end of the inoculating needle 31 is provided with an inoculating loop 31 b.
In order to facilitate the processing and assembly and improve the sealing performance, the box body part 160 and the collecting cup 11 are in a round bottle cap-shaped structure in the embodiment; the upper edge of the box body part 160 and the lower edge of the collecting cup 11 are buckled with each other;
wherein, the inner side of the lower edge of the collecting cup 11 extends downwards to form a leakage-proof ring 111, when the collecting cup 11 is buckled at the opening of the reagent box 16, the leakage-proof ring 111 extends into the inner cavity of the storage cup 15 and is close to the cavity wall of the storage cup 15; the bottom of the connecting cylinder 15b is dug down to be lower than the bottom of the storage cup 15 so that when the slider 14 is moved upward, the liquid can flow from the bottom of the storage cup 15 to the filling hole 15a in the center of the connecting cylinder 15 b.
Specifically, a guide post 14c is provided on the upper side of the seal portion 14 a; the pressure-bearing part 12 is annular and is sleeved on the guide post 14 c; the first compression spring 13 is fitted over the guide post 14 c.
The guide post 14c can maintain the relative positional relationship between the first compression spring 13 and the pressure receiving member 12, and is convenient for assembly and use.
To meet the requirements of different collection capacities, the collection cup 11 comprises a top receptacle 110; the collecting cup 11 is provided in plurality, and each collecting cup 11 has a receiving portion 110 having a different volume.
The container can adopt a common quantitative volume and standard capacity so as to be suitable, such as common 20m and 40 ml.
In this embodiment, the culture cassette 20 has a housing structure with an open upper side; a culture tank 20a for containing a culture medium is provided in the middle of the culture box 20, and the lower half of the introduction passage 202 is formed by a tank structure communicated to the culture tank 20 a; the rear side of the culture tank 20a is provided with an object placing tank 20b, and the bottom of the object placing tank 20b is provided with a metal block or a magnetic block 208 which can attract the magnet 32.
As shown in fig. 5 and 6, in order to realize one-to-two or more tests and facilitate the completion of multiple tests by one-time collection, a plurality of connecting parts 161 are provided on the reagent kit 16; the hollow part of each connecting part 161 is internally provided with mutually independent contact cavities 163, the bottoms of the contact cavities 163 are respectively communicated with the mounting cavity 16a through the diversion holes 16b, and the bottom of each contact cavity 163 is lower than the bottom of the mounting cavity 16 a; magnetic scribe head 3 is disposed within contact cavity 163 with inoculating needle 31 facing the bottom of contact cavity 163.
To facilitate control of the flow direction of the sample and storage of the reagent, a partition 164 is provided inside the mounting chamber 16 a; the partition plate 164 is divided into an inner groove 165 corresponding to the injection hole 15a inside and an outer groove 166 outside in the mounting cavity 16 a; the inner groove 165 extends toward the guide hole 16b and communicates with the contact chamber 163.
The reagent adsorption sponge is arranged in the outer groove 166, and when the collection cup 11 is buckled in the reagent box 16, the bottom of the reagent box 16 is close to the reagent adsorption sponge.
The inner grooves 165 connected to the respective connecting portions 161 are combined and communicated at the center of the mounting chamber 16 a; the bottom of the inner groove 165 connected to each connecting portion 161 is a flat inclined surface, and the side with the higher inclined surface faces the center of the mounting cavity 16 a.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the protection scope of the present application, and although the present application is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present application without departing from the spirit and scope of the technical solutions of the present application.
Claims (10)
1. A totally-enclosed microorganism inoculation lineation acquisition culture kit is characterized by comprising a collection assembly (1), a culture assembly (2) and a magnetic lineation head (3);
the collection assembly (1) comprises: the collecting cup (11), the pressure bearing part (12), the first compression spring (13), the sliding part (14), the storage cup (15) and the reagent box (16) are arranged from top to bottom in sequence;
the reagent kit (16) comprises a kit body part (160) provided with a mounting cavity (16a) and a hollow connecting part (161) arranged at one side of the mounting cavity (16 a); a flow guide hole (16b) communicated with the connecting part (161) is formed in the inner wall of the mounting cavity (16 a); the inner cavity of the connecting part (161) is communicated with the mounting cavity (16a) through a diversion hole (16 b); a lead-out opening is formed in the outer side of the connecting part (161), a first baffle (162) is installed at the lead-out opening, and the lead-out opening is blocked by the first baffle (162) through a rotating shaft and a torsion spring;
the shape of the storage cup (15) is matched with that of the installation cavity (16a), the upper end of the storage cup (15) is opened, the center of the bottom of the storage cup is provided with an injection hole (15a), a connecting cylinder (15b) is arranged outside the injection hole (15a), the cylinder wall of the connecting cylinder (15b) is provided with a plurality of connecting holes (15c), and the cylinder wall of the connecting cylinder (15b) is provided with a drainage hole or a drainage groove (15d) connected to the cavity bottom of the installation cavity (16 a);
the sliding piece (14) is arranged in the connecting cylinder (15b) in a vertically sliding manner and comprises a sealing part (14a) with the middle part capable of abutting against and sealing the injection hole (15a), and a pressing part (14b) capable of penetrating out of the injection hole (15a) is arranged on the lower side of the sealing part (14 a);
the upper end of the pressure-bearing part (12) props against the collecting cup (11), and the upper end of the first compression spring (13) props against the lower end of the pressure-bearing part (12) and props against the sliding part (14); the outer side of the pressure bearing piece (12) is provided with a convex part (12a) which can be buckled in the connecting hole (15 c);
the collecting cup (11) is buckled at the opening of the reagent box (16), the bottom of the collecting cup (11) is propped against the pressure-bearing part (12), and the sealing part (14a) is pressed against the injection hole (15a) after the first compression spring (13) is compressed;
the culture component (2) is close to the connecting part (161) and is arranged at one side of the outlet;
the culture component (2) comprises a culture box (20) and a box cover (21); the box cover (21) is detachably buckled on the culture box (20), and a closed culture cavity (201) is formed in the box cover;
a leading-in channel (202) is arranged on one side of the culture cavity (201) facing the connecting part (161), the leading-in channel (202) is opposite to the leading-out port, a second baffle (203) is arranged at the outlet of the leading-in channel, and the leading-in channel (202) is blocked by the second baffle (203) through a rotating shaft and a torsion spring;
the magnetic marking head (3) is arranged on the inner side of the connecting part (161) and is close to the outlet;
the magnetic scribing head (3) comprises: a supporting cylinder (30) and an inoculating needle (31) which is sleeved at the lower end of the supporting cylinder (30) and can slide up and down; a magnet (32) embedded in the upper end of the support cylinder (30); a second compression spring (33) with two ends abutting against the magnet (32) and the inoculating needle (31).
2. The fully-closed microorganism inoculation, lineation, collection and culture kit according to claim 1, is characterized in that a step hole (30a) is formed at the lower opening of the support cylinder (30), and the size of the upper end of the inoculation needle (31) is larger than the aperture of the step hole (30a) so as to be clamped on the step of the limiting step hole (30 a); the upper end of the inoculating needle (31) is provided with a spring installation groove (31a) capable of accommodating the lower end of the second compression spring (33), and the lower end of the inoculating needle (31) is provided with an inoculating loop (31 b).
3. A totally enclosed microbial inoculation and streaking collection and culture kit as claimed in claim 1, wherein the box body portion (160) and the collection cup (11) are of a round bottle cap-like structure; the upper edge of the box body part (160) and the lower edge of the collecting cup (11) are buckled with each other;
the inner side of the lower edge of the collecting cup (11) extends downwards to form a leakage-proof ring (111), and when the collecting cup (11) is buckled at the opening of the reagent kit (16), the leakage-proof ring (111) extends into the inner cavity of the storage cup (15) and is close to the wall of the storage cup (15);
the bottom of the connecting cylinder (15b) is dug downwards until the bottom is lower than the bottom of the storage cup (15), so that when the sliding piece (14) moves upwards, liquid can flow from the bottom of the storage cup (15) to the filling hole (15a) in the center of the connecting cylinder (15 b).
4. A totally enclosed microbial inoculation streak collection culture kit according to claim 1, wherein the upper side of the sealing portion (14a) is provided with a guide post (14 c); the pressure-bearing piece (12) is annular and sleeved on the guide post (14 c); the first compression spring (13) is sleeved on the guide post (14 c).
5. A fully enclosed microbial inoculation streaking harvesting culture kit as claimed in claim 1, characterised in that the collection cup (11) includes a top receptacle (110); a plurality of collecting cups (11) are provided, and each collecting cup (11) has a receiving part (110) with different volumes.
6. A totally enclosed microbial inoculation, streaking, harvesting and cultivation kit as claimed in claim 1, characterised in that the cultivation box (20) is of a shell structure with an open upper side; a culture tank (20a) for containing a culture medium is arranged in the middle of the culture box (20), and the lower half part of the leading-in channel (202) is formed by a tank structure communicated with the culture tank (20 a);
an object placing groove (20b) is arranged at the rear side of the culture groove (20a), and a metal block or a magnetic block (208) which can attract the magnet (32) is arranged at the bottom of the object placing groove (20 b).
7. A totally enclosed microbial inoculation streaking harvesting culture kit as claimed in claim 1, wherein the kit (16) is provided with a plurality of connections (161);
the hollow part of each connecting part (161) is internally provided with mutually independent contact cavities (163), the bottoms of the contact cavities (163) are respectively communicated with the installation cavity (16a) through flow guide holes (16b), and the height of the bottoms of the contact cavities (163) is lower than that of the bottoms of the installation cavity (16 a); the magnetic scribing head (3) is arranged in the contact cavity (163), and the inoculating needle (31) faces to the bottom of the contact cavity (163).
8. A totally enclosed microbial inoculation and streaking harvesting and culturing kit as claimed in claim 7, wherein the inside of the installation cavity (16a) is provided with a partition (164); the partition plate (164) is divided into an inner groove (165) corresponding to the injection hole (15a) on the inner side and an outer groove (166) on the outer side in the mounting cavity (16 a); the inner groove (165) extends toward the flow guide hole (16b) and communicates with the contact chamber (163).
9. A fully enclosed microbial inoculation streaking harvesting culture kit as claimed in claim 8 in which the reagent adsorption sponge is located within the outer trough (166) and the bottom of the reagent cartridge (16) is adjacent the reagent adsorption sponge when the collection cup (11) is retained within the reagent cartridge (16).
10. The totally enclosed microorganism inoculation, streaking, collecting and culturing kit according to claim 8, wherein the inner grooves (165) connected to the respective connecting portions (161) are combined and communicated at the center of the installation cavity (16 a); the bottom of the inner groove (165) connected with each connecting part (161) is a flat inclined plane, and the higher side of the inclined plane faces the center of the mounting cavity (16 a).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111213292.3A CN113969237B (en) | 2021-10-19 | 2021-10-19 | Totally-enclosed microorganism inoculation streaking acquisition and culture kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111213292.3A CN113969237B (en) | 2021-10-19 | 2021-10-19 | Totally-enclosed microorganism inoculation streaking acquisition and culture kit |
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| Publication Number | Publication Date |
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| CN113969237A true CN113969237A (en) | 2022-01-25 |
| CN113969237B CN113969237B (en) | 2024-04-26 |
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| CN202111213292.3A Active CN113969237B (en) | 2021-10-19 | 2021-10-19 | Totally-enclosed microorganism inoculation streaking acquisition and culture kit |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060040340A1 (en) * | 2004-08-17 | 2006-02-23 | Nathan Greene | Microorganism specimen storage, hydrating, transfer and applicator device |
| CN103194385A (en) * | 2013-03-14 | 2013-07-10 | 华珊微生物科技有限公司 | Microbial sample processing system |
| CN204022831U (en) * | 2014-08-22 | 2014-12-17 | 华珊微生物科技有限公司 | A kind of micro-biological samples streak inoculation device |
| CN105779278A (en) * | 2016-03-29 | 2016-07-20 | 齐鲁工业大学 | Convenient microorganism culture inoculating tube |
-
2021
- 2021-10-19 CN CN202111213292.3A patent/CN113969237B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060040340A1 (en) * | 2004-08-17 | 2006-02-23 | Nathan Greene | Microorganism specimen storage, hydrating, transfer and applicator device |
| CN103194385A (en) * | 2013-03-14 | 2013-07-10 | 华珊微生物科技有限公司 | Microbial sample processing system |
| CN204022831U (en) * | 2014-08-22 | 2014-12-17 | 华珊微生物科技有限公司 | A kind of micro-biological samples streak inoculation device |
| CN105779278A (en) * | 2016-03-29 | 2016-07-20 | 齐鲁工业大学 | Convenient microorganism culture inoculating tube |
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| CN113969237B (en) | 2024-04-26 |
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