CN221484948U - Special dyeing device capable of saving reagent and facilitating tablet taking for immunofluorescence cell climbing tablet - Google Patents

Abstract

The utility model discloses a special dyeing device capable of saving reagents and facilitating tablet taking for an immunofluorescent cell climbing tablet, which is provided with a box body (1) and a box cover (2), wherein a plurality of incubation small chambers (3) are adjacently arranged in the box body (1), the incubation small chambers (3) are rectangular cavities with inner arc surfaces at the bottom surfaces, the length and the height of the rectangular cavities are larger than the diameter of the immunofluorescent cell climbing tablet by 1-1.5mm and the width of the rectangular cavities by 0.5-1mm, the height of the inner arc surfaces is smaller than or equal to the radius of the immunofluorescent cell climbing tablet, the upper edges of two length surfaces of the rectangular cavities are provided with oppositely arranged reaming surfaces (3-1), and the two reaming surfaces (3-1) form a funnel-shaped opening. The liquid reagent in each incubation cell is used for guaranteeing the minimum dosage required by staining, waste caused by excessive reagent is avoided, and the funnel-shaped opening is convenient for taking out the cell climbing sheet vertical in the incubation cell, so that the phenomenon of damaging a sample due to sheet taking is avoided.

Description

A special staining device for immunofluorescence cell slides that saves reagents and facilitates slide retrieval

Technical Field

The utility model relates to the field of biological experimental materials, in particular to a special staining device for immunofluorescence cell slides which can save reagents and is convenient for slide taking.

Background technique

Immunofluorescence is the process of labeling fluorescent pigments that do not affect the activity of antigens and antibodies on the cell membranes, organelles and nuclei of antibodies (or antigens). After binding to the corresponding antigens (or antibodies), a specific fluorescent reaction result is presented under a fluorescence microscope. The results of the fluorescent reaction can intuitively reflect the localization of proteins in cells, changes in cell morphology and cell structure, and are an important basis for biological experimenters to judge the process of cell biology. The specific method of immunofluorescence is to add the labeled specific fluorescent antibody (or antigen) directly to the antigen (or antibody) specimen, stain it at a certain temperature and time, wash away the excess fluorescent antibody (or antigen) that does not participate in the reaction with water, dry it at room temperature, and seal it for microscopic examination. The existing staining devices include cell culture plates and laser confocal culture dishes. The cell culture plate is a plate with multiple circular holes, such as a 6-well plate, etc.; the laser confocal culture dish is a plate with multiple circular holes on the bottom of the dish. During staining, the cell slide carrying the antigen (or antibody) is placed in a circular hole groove (incubation chamber), and then the labeled specific fluorescent antibody (or antigen) reagent is added for incubation (staining). Usually, the added specific fluorescent antibody (or antigen) needs to be submerged to a certain height of the cell slide. The diameter of the circular hole groove of the existing 6-well plate is 35mm, while the diameter of the cell slide is usually less than 25mm. Although the diameter difference between the two can ensure the removal of the cell slide, there is a phenomenon of waste of reagents; the diameter of the circular hole groove of the laser confocal special culture dish is basically consistent with the cell slide, but the depth is shallow. During staining, the labeled specific fluorescent antibody (or antigen) and other liquid reagents are added to the culture dish. The amount added is not fixed and only one reagent can be added at a time, which also wastes reagents. In addition, the thickness of the cell slide is usually only 0.14-0.17mm. The cell slides of the two existing staining devices are tightly attached to the bottom surface, which makes it difficult to take the slide, and sometimes the sample is damaged due to taking the slide.

Summary of the invention

The utility model aims to solve the above technical problems existing in the prior art and provides a special staining device for immunofluorescence cell slides which can save reagents and is convenient for slide taking.

The technical solution of the utility model is: a special staining device for immunofluorescence cell slides which can save reagents and is convenient for taking slides, is provided with a box body and a box cover, a plurality of adjacently arranged incubation chambers are arranged in the box body, the incubation chambers are rectangular cavities with a bottom surface as an inner arc surface, the length and height of the rectangular cavity are greater than the diameter of the immunofluorescence cell slide of 1-1.5mm and the width of 0.5-1mm, the height of the inner arc surface is less than or equal to the radius of the immunofluorescence cell slide, the upper edges of the two length surfaces of the rectangular cavity are provided with relatively arranged flaring surfaces, and the two flaring surfaces constitute a funnel-shaped opening.

The preferred technical solution is that the length and height of the rectangular cavity are 26 mm, the width is 1 mm, the height of the inner arc surface is 13 mm, the maximum diameter of the funnel-shaped opening is 4 mm, and the height is 4 mm.

The utility model is to set a plurality of adjacently arranged incubation chambers in a box body, each incubation chamber is a rectangular cavity with an inner arc surface at the bottom, and the length, height, width of the rectangular cavity and the height of the inner arc surface are set so that after the immunofluorescence cell slide is placed in the incubation chamber, its bottom edge just fits on the inner arc surface, so that it can stand vertically in the incubation chamber stably for staining. The liquid reagent in each incubation chamber is to ensure the minimum dosage required for staining, avoiding waste caused by using too much reagent; at the same time, multiple incubation chambers can select the same or different liquid reagents according to needs, which is convenient to use; the funnel-shaped opening is set to facilitate the removal of the cell slide vertically (not tightly fitted with the bottom surface) in the incubation chamber, avoiding the phenomenon of damaging the sample due to taking the slide.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a schematic diagram of the three-dimensional structure of an embodiment of the utility model.

FIG. 2 is a schematic diagram of the structure of an incubation chamber according to an embodiment of the present utility model.

FIG. 3 is a top view of the inner structure of the box body of the utility model embodiment.

FIG. 4 is an A-A view of FIG. 3 .

FIG. 5 is a schematic diagram of the use state of an embodiment of the utility model.

Detailed ways

The special staining device for immunofluorescence cell slides of the utility model, which can save reagents and is convenient for taking slides, is shown in Figures 1, 2, 3, and 4. It is provided with a box body 1 and a box cover 2. The box body 1 and the box cover 2 can adopt an opening structure such as a hinge. The outer wall of the box body 1 is in close contact with the inner wall of the box cover 2, which effectively hinders the flow of gas and reduces bacterial contamination in the air. The box body 1 and the box cover 2 are both made of polypropylene (PP) material that can be sterilized at high temperature and reused. 10 adjacently arranged incubation chambers 3 can be processed in the box body 1 by injection molding or the like. The incubation chamber 3 is a rectangular cavity with an inner arc surface on the bottom surface. The length and height of the rectangular cavity are greater than the diameter of the immunofluorescence cell slide 1-1.5mm and the width 0.5-1mm. The height of the inner arc surface is less than or equal to the radius of the immunofluorescence cell slide, so that the bottom edge of the immunofluorescence cell slide 4 is exactly coincident with the inner arc surface after being placed in the incubation chamber 3, so that it can stand vertically in the incubation chamber stably for staining, as shown in Figure 5. There will not be too much space between the cell climbing sheet and the side wall of the incubation chamber 3, which can ensure the minimum dose required for staining and avoid waste caused by using too much reagent. At the same time, multiple incubation chambers are not connected to each other, and the same or different liquid reagents can be selected as needed, which is convenient to use; the upper edges of the two length surfaces of the rectangular cavity have relatively arranged expansion surfaces 3-1, and the two expansion surfaces 3-1 form a funnel-shaped opening, which is convenient for the cell climbing sheet to be smoothly inserted and removed. If the diameter of the cell climbing sheet is 25mm and the thickness is 0.17mm, the preferred technical solution is that the length and height of the rectangular cavity are 26mm, the width is 1mm, the height of the inner arc surface is 13mm, and the maximum diameter of the funnel-shaped opening is 4mm and the height is 4mm.

Claims (2)

1. Can save reagent and be convenient for get special dyeing apparatus of immunofluorescence cell climbing piece of piece, its characterized in that: be equipped with box body (1) and lid (2), have a plurality of adjacent ranges of incubating cell (3) in box body (1), it is the rectangular cavity of interior cambered surface to incubate cell (3), the length and the height of rectangular cavity are greater than immunofluorescence cell climbing piece diameter 1-1.5mm, width 0.5-1mm, the height of interior cambered surface is less than or equal to immunofluorescence cell climbing piece's radius, the upper edge of two length faces of rectangular cavity has reaming face (3-1) of relative setting, and two reaming faces (3-1) constitute the funnel opening.

2. The reagent-saving and convenient-to-take special staining device for immunofluorescent cell slide according to claim 1, wherein the staining device is characterized in that: the length and the height of the rectangular cavity are 26mm and 1mm, the height of the inner arc surface is 13mm, the maximum diameter of the funnel-shaped opening is 4mm, and the height is 4mm.