CN204022831U - A kind of micro-biological samples streak inoculation device - Google Patents

A kind of micro-biological samples streak inoculation device Download PDF

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Publication number
CN204022831U
CN204022831U CN201420476186.3U CN201420476186U CN204022831U CN 204022831 U CN204022831 U CN 204022831U CN 201420476186 U CN201420476186 U CN 201420476186U CN 204022831 U CN204022831 U CN 204022831U
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China
Prior art keywords
culture plate
streak inoculation
micro
biological samples
plate face
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Expired - Fee Related
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CN201420476186.3U
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Chinese (zh)
Inventor
陈尚斌
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Hunan numerous mountains Medical Devices Co., Ltd.
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HUA SHAN MICROBIOLOGICAL TECHNOLOGY Ltd
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Abstract

The utility model relates to a kind of micro-biological samples streak inoculation device, comprise culture plate, streak inoculation pen, sample liquefaction package and culture dish base, described culture plate is bilateral structure, comprise the first culture plate face and the second culture plate face, on described culture plate face, vertical direction arranges bossed dividing plate, the culture plate face at its place is divided into two regions by described dividing plate, described culture plate is arranged on described culture dish base, described sample liquefaction package bottom is connected with culture dish base, described streak inoculation pen is arranged at described culture plate side, a described streak inoculation top and external mechanical palmistry connect.The utility model project organization is reasonable, can under the cooperation of micro-biological samples treatment unit, efficiently complete fast streak inoculation, replaces manual operations, ensures Biosafety.

Description

A kind of micro-biological samples streak inoculation device
Technical field
The utility model relates to micro-biological samples treatment unit, concrete, relates to a kind of micro-biological samples streak inoculation device.
Background technology
A large amount of micro-biological samples will be faced and process to microorganism field every day.The blood microorganism sample process that accounts for clinical 20%-25% realizes automation equipment and stdn already.But, accounting for clinical more than 70% non-blood microorganism sample (as urine, ight soil, sputum, body fluid, swab (secretory product), and water quality, food, movement etc.) processes and but still continues to use for many years traditional-handwork operation of continuity.
Contriver has applied for being entitled as a kind of application for a patent for invention of micro-biological samples treatment unit in 2013, now obtain Chinese invention patent mandate, the patent No. is: ZL201310080568.4, it has solved length consuming time in traditional non-blood microorganism sample process manual operations, efficiency is low, infection risk is high problem.Simultaneously in this patent of invention, a kind of micro-biological samples streak inoculation device being used in conjunction with micro-biological samples treatment unit has also been proposed, overcome in prior art streak inoculation by hand take long, efficiency is low, labour cost is high, operator with have infection risk, people's randomness and nonstandardized technique operation can affect microbionation effect with the sample close contact of pathogenic bacterium, cause the technological deficiency of irreversible malpractice.But, owing to also having certain deficiency in its disclosed classification inoculation apparatus, as to inoculate plate be rectangle bilateral structure, single job can only be poured at most two kinds of different culture medias, cannot meet for clinical great amount of samples and detect needs, the plate of rectangular configuration inoculation simultaneously also has some limitations in clinical practice demand.
Utility model content
For overcoming the deficiencies in the prior art, the utility model proposes a kind of micro-biological samples streak inoculation device that can carry out streak inoculation on multiple different culture media simultaneously.
The technical measures of implementing the utility model above-mentioned purpose and adopt are as described below:
A kind of micro-biological samples streak inoculation device, comprise culture plate, streak inoculation pen, sample liquefaction package and culture dish base, it is characterized in that: described culture plate is bilateral structure, comprise the first culture plate face and the second culture plate face, on described culture plate face, vertical direction arranges bossed dividing plate, the culture plate face at its place is divided into two regions by described dividing plate, described culture plate is arranged on described culture dish base, described sample liquefaction package bottom is connected with culture dish base, described streak inoculation pen has two nibs, be arranged at respectively described culture plate both sides.
Wherein, described culture plate is double-sided circular structure, comprises the first culture plate face and the second culture plate face, and described the first culture plate face and the second culture plate face arrange back-to-back.
Preferably, the culture plate face at its place is divided into two regions by described dividing plate.
Preferably, described dividing plate is located on the second culture plate face.
Wherein, described culture plate circumference top is provided with the culture plate seat of honour, and described culture plate circumference bottom is provided with anchor, is provided with streak inoculation and holds in the works fixed block on described anchor.
Wherein, be provided with a streak inoculation exit and entrance on the described culture plate seat of honour, described streak inoculation pen is arranged on culture plate both sides by a streak inoculation exit and entrance through the culture plate seat of honour.
Preferably, on described growth plate face, be horizontally arranged with the fixing muscle of many projections.
Wherein, also comprise two culture plate lids, described culture plate lid is separately positioned on the first culture plate face and the second culture plate face.
Preferably, described culture plate periphery is higher than its plate face 4mm, and diameter is 9cm.
Wherein, sample liquefaction package comprises the liquefier cup that is provided with size of a sample graduated scale mark, extend in described liquefier cup for stirring the stirring rod of sample, for the liquefier lid of liquefier cup described in capping and the stirring rod handle being connected with stirring rod with the rotation of driving stirring rod.Sample liquefaction package below is provided with liquefier cup pipe box, and liquefier cup pipe box is connected by sample diversion trench with culture dish base.Described culture dish base is connected with described liquefaction package, is transparent cavity.
Adopt the technical solution of the utility model, can obtain following technique effect:
One, the utility model can be inoculated by while fast scribing on the substratum of number of different types, increases work efficiency.
Two, the culture plate that the utility model adopts, adopts large-area culture plate can observe more clearly form and the color of bacterial colony, and zone of hemolysis, makes it more adapt to clinical practice needs.
Three, on the growth plate face that the utility model adopts, be horizontally arranged with the fixing muscle of many projections, can increase the sticking power of substratum, prevent that substratum from coming off in transit.
Four, the utility model can be used in conjunction with micro-biological samples treatment unit (patent No.: ZL201310080568.4), make micro-biological samples sampling, streak inoculation, desalination separate bacterium colony full process automatization operation, rapidly and efficiently, safe and reliable, replace by hand, realize the standardized work of micro-biological samples streak inoculation.
Brief description of the drawings
Fig. 1 is according to the schematic diagram of micro-biological samples streak inoculation device of the present utility model;
Fig. 2 is according to the exploded perspective view of micro-biological samples streak inoculation device of the present utility model;
Fig. 3 is according to micro-biological samples streak inoculation device culture plate the first culture plate face schematic diagram of the present utility model;
Fig. 4 is according to micro-biological samples streak inoculation device culture plate the second culture plate face schematic diagram of the present utility model;
Fig. 5 is according to micro-biological samples streak inoculation device culture dish base of the present utility model and sample liquefaction package connectivity part structural representation;
Fig. 6 is according to micro-biological samples streak inoculation device streak inoculation pen of the present utility model movement locus schematic diagram in the course of the work.
Reference numeral:
1-culture dish base; 2-culture plate; 3-streak inoculation pen; 4-culture plate lid; 5-stirring rod handle; 6-stirring rod; 7-liquefier lid; 8-liquefier cup; 9-liquefier cup pipe box; 10-sample liquefaction package; 11-sample diversion trench; 12-the first culture plate face; 13-fixes muscle; 14-fixed block; A 15-streak inoculation exit and entrance; 16-anchor; The 17-culture plate seat of honour; 18-the second culture plate face; 19-dividing plate; 20-inclined-plane passage; The different positions of A, B, C-inoculation streak inoculation pen.
Embodiment
Describe embodiment of the present utility model below in detail, the example of described embodiment is shown in the drawings, and the embodiment describing by reference to accompanying drawing is exemplary, is intended to for explaining the utility model, and can not be interpreted as restriction of the present utility model.
In description of the present utility model, it will be appreciated that, term " centre ", " longitudinally ", " laterally ", " diameter ", " width ", " thickness ", " on ", D score, " front ", " afterwards ", " left side ", " right side ", " vertically ", " level ", " top ", " end " " interior ", " outward ", " clockwise ", orientation or the position relationship of instructions such as " counterclockwise " are based on orientation shown in the drawings or position relationship, only the utility model and simplified characterization for convenience of description, instead of device or the assembly of instruction or hint indication must have specific orientation, with specific orientation structure and operation, therefore can not be interpreted as restriction of the present utility model.
In the utility model, unless otherwise clearly defined and limited, the terms such as term " installation ", " being connected ", " connection ", " fixing " should be interpreted broadly, and for example, can be to be fixedly connected with, and can be also to removably connect, or connect integratedly; Can be mechanical connection, can be also electrical connection; Can be to be directly connected, also can indirectly be connected by intermediary, can be the connection of two component internal.For the ordinary skill in the art, can understand as the case may be the concrete meaning of above-mentioned term in the utility model.
In the utility model, unless otherwise clearly defined and limited, First Characteristic Second Characteristic it " on " or D score can comprise that the first and second features directly contact, also can comprise that the first and second features are not directly contacts but by the other feature contact between them.And, First Characteristic Second Characteristic " on ", " top " and " above " comprise First Characteristic directly over Second Characteristic and oblique upper, or only represent that First Characteristic level height is higher than Second Characteristic.First Characteristic Second Characteristic " under ", " below " and " below " comprise First Characteristic under Second Characteristic and tiltedly, or only represent that First Characteristic level height is less than Second Characteristic.
As shown in Figure 1, Figure 3, the utility model discloses a kind of micro-biological samples streak inoculation device, comprise culture plate 2, streak inoculation pen 3, sample liquefaction package 10 and culture dish base 1.
Culture plate 2 is circular double-face structure, and culture plate 2 peripheries are higher than its plate face 4mm, and diameter is 9cm, comprises that the first culture plate face 12 and the second culture plate face 18, the first culture plate faces 12 and the second culture plate face 18 arrange back-to-back.The fixing muscle 13 that is all horizontally arranged with many projections on described culture plate 2 plate faces, described fixing muscle 13 can effectively increase the sticking power of substratum, prevents that substratum from coming off.On the second culture plate face 18 of described culture plate 2, vertical direction arranges bossed dividing plate 19 (as shown in Figure 4), described dividing plate 19 is two regions by the second culture plate face 18 equipartitions, can on the second culture plate face 18, pour into two kinds of different substratum simultaneously and inoculate line, increase work efficiency.On the first culture plate face 12 and the second culture plate face 18, be also respectively arranged with culture plate lid 4, culture plate lid 4 is transparent plastic cover.
Be provided with the culture plate seat of honour 17 on culture plate 2 circumference tops, in the mid-way of 17 both sides, the culture plate seat of honour, be provided with a streak inoculation exit and entrance 15, the passage of the culture plate of coming in and going out is provided for it in streak inoculation pen 3 working process, and rule necessary space is provided for its inoculation.Be provided with anchor 16 in culture plate 2 circumference bottoms, described culture plate 2 is fixed on culture dish base 1 by anchor 16.Meanwhile, in the mid-way of anchor 16 both sides, be respectively arranged with fixed block 14 (as shown in Figure 3), for the lower end of fixing streak inoculation pen 3.
Streak inoculation pen 3 is bipitch structure, is arranged on culture plate 2 both sides by a streak inoculation exit and entrance 15 that is arranged on the culture plate seat of honour.When work, inoculation streak inoculation pen 3 apical positions and external mechanical palmistry connect.
As shown in Figure 2, sample liquefaction package 10 comprises the liquefier cup 8 that is provided with size of a sample graduated scale mark, extend in described liquefier cup 8 for stirring the stirring rod 6 of sample, for the liquefier lid 7 of liquefier cup 8 described in capping and the stirring rod handle 5 being connected with stirring rod 6 with the rotation of driving stirring rod.Sample liquefaction package 10 belows are provided with liquefier cup pipe box 9, and liquefier cup pipe box 9 is connected by sample diversion trench 11 with culture dish base 1.Described culture dish base 1 is connected with described liquefaction package 10, is transparent cavity.
Introduce the detailed process that in the present embodiment, micro-biological samples streak inoculation device carries out streak inoculation below:
Before assembling, the first required substratum at the two-sided perfusion 4.0mm of culture plate 2 thickness, because the second culture plate face 18 is divided into two intervals by dividing plate 19, can specifically need to pour into different types of substratum in two intervals of the first culture plate face 12 and the second culture plate face 18 according to what detect, culture plate 2 can carry out at most the perfusion of 3 kinds of different sorts substratum simultaneously.Then culture plate lid 4 is placed on behind culture plate 2 two sides, culture plate 2 is arranged on culture dish base 1.Two-sided streak inoculation pen 3 is slowly inserted to culture plate 2 by a streak inoculation exit and entrance 15, streak inoculation pen 3 bottom transfering loops are placed in the sample diversion trench 11 in culture dish base 1.
Such as, by manually micro-biological samples (sputum, urine, stool, swab, body fluid etc.) and relevant nutritive medium or Digestive system being put into the described sample liquefier cup 8 that is provided with size of a sample scale mark, stirring rod 6 is connected with stirring rod handle 5, be inserted in again liquefier lid 7, then liquefier lid 7 is placed on described sample liquefier cup 8, then described sample liquefier cup 8 is placed in described liquefier cup pipe box 9 (as shown in Figure 2).
When automated operation, micro-biological samples streak inoculation device assembly of the present utility model (as shown in Figure 1) is put into the specimen holder of micro-biological samples treatment unit (patent of invention number: ZL201310080568.4), the liquefaction mechanical manipulator of micro-biological samples treatment unit can move to described liquefier cup 8 tops at once, and draw stirring rod handle 5, drive stirring rod 6 to stir micro-biological samples and the annex solution of liquefier cup 8, make sample liquefaction, homogenizing and mixing stirs evenly.In the time of streak inoculation, the compressing mechanical manipulator of micro-biological samples treatment unit can move to liquefier cup 8 tops, slowly depress, the plastic bottoms pressurized of described sample liquefier cup 8 is broken, the micro-biological samples being liquefied flows into the sample diversion trench 11 of culture dish base 1 through sample liquefier glass holder pipe 9 inclined-plane passages 20, two-sided streak inoculation pen 3 ends are soaked in the sample of sample diversion trench 11.This moment, the mechanical manipulator of full automatic microorganism sample processing apparatus just drives two-sided streak inoculation pen (3) on the substratum of the synchronous culture plate (2) at 9 centimeters of culture plates of circular double-face diameter, to carry out by setup program streak inoculation under the protection of culture plate lid (4) simultaneously.Concrete, the line mechanical manipulator meeting driving mechanical cleft hand of instrument is clamped the upper Square handle of described two-sided streak inoculation pen 3, upwards be drawn in the two-sided media surface of described culture plate 2, then mechanical manipulator starts in y direction, streak inoculation pen 3 to be drawn, and slightly move back and forth in x direction, make streak inoculation pen 3 upwards swinging in lifting process, make streak inoculation pen 3 in different location status, as A, B, C position, transfering loop microbe inoculation sample and line on two-sided substratum of two-sided streak inoculation pen 3 bottoms, specifically referring to Fig. 6.
Whole streak inoculation process, by the automated operation of full automatic microorganism sample processing apparatus computer control mechanical manipulator, replaces handwork, ensures Biosafety.
Although illustrated and described embodiment of the present utility model above, be understandable that, above-described embodiment is exemplary, can not be interpreted as restriction of the present utility model, those of ordinary skill in the art can change above-described embodiment in the situation that not departing from principle of the present utility model and aim in scope of the present utility model, amendment, replacement and modification.

Claims (9)

1. a micro-biological samples streak inoculation device, comprise culture plate, streak inoculation pen, sample liquefaction package and culture dish base, it is characterized in that: described culture plate is bilateral structure, comprise the first culture plate face and the second culture plate face, on described culture plate face, vertical direction arranges bossed dividing plate, the culture plate face at its place is divided into two regions by described dividing plate, described culture plate is arranged on described culture dish base, described sample liquefaction package bottom is connected with culture dish base, described streak inoculation pen has two nibs, be arranged at respectively described culture plate both sides.
2. a kind of micro-biological samples streak inoculation device according to claim 1, is characterized in that: described culture plate is double-sided circular structure, comprises the first culture plate face and the second culture plate face, and described the first culture plate face and the second culture plate face arrange back-to-back.
3. a kind of micro-biological samples streak inoculation device according to claim 1, is characterized in that: the culture plate face at its place is divided into two regions by described dividing plate.
4. a kind of micro-biological samples streak inoculation device according to claim 1, is characterized in that: described dividing plate is located on the second culture plate face.
5. a kind of micro-biological samples streak inoculation device according to claim 2, it is characterized in that: described culture plate circumference top is provided with the culture plate seat of honour, described culture plate circumference bottom is provided with anchor, is provided with streak inoculation and holds in the works fixed block on described anchor.
6. a kind of micro-biological samples streak inoculation device according to claim 5, it is characterized in that: on the described culture plate seat of honour, be provided with a streak inoculation exit and entrance, described streak inoculation pen is arranged on culture plate both sides by a streak inoculation exit and entrance through the culture plate seat of honour.
7. a kind of micro-biological samples streak inoculation device according to claim 1, is characterized in that: the fixing muscle that is horizontally arranged with many projections on described growth plate face.
8. a kind of micro-biological samples streak inoculation device according to claim 1, is characterized in that: also comprise two culture plate lids, described culture plate lid is separately positioned on the first culture plate face and the second culture plate face.
9. a kind of micro-biological samples streak inoculation device according to claim 2, is characterized in that: described culture plate periphery is higher than its plate face 4mm, and diameter is 9cm.
CN201420476186.3U 2014-08-22 2014-08-22 A kind of micro-biological samples streak inoculation device Expired - Fee Related CN204022831U (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104862215A (en) * 2015-05-29 2015-08-26 重庆道鑫生物科技有限公司 Microbial sample processing and scribing system and method
CN108587885A (en) * 2018-04-23 2018-09-28 山东省农业科学院农业资源与环境研究所 A kind of micro-biological samples streak inoculation device and method
CN113969237A (en) * 2021-10-19 2022-01-25 微尔创(武汉)医疗科技有限公司 Totally closed formula microorganism inoculation marking off gathers and cultivates external member
CN113969237B (en) * 2021-10-19 2024-04-26 微尔创(武汉)医疗科技有限公司 Totally-enclosed microorganism inoculation streaking acquisition and culture kit

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104862215A (en) * 2015-05-29 2015-08-26 重庆道鑫生物科技有限公司 Microbial sample processing and scribing system and method
CN108587885A (en) * 2018-04-23 2018-09-28 山东省农业科学院农业资源与环境研究所 A kind of micro-biological samples streak inoculation device and method
CN113969237A (en) * 2021-10-19 2022-01-25 微尔创(武汉)医疗科技有限公司 Totally closed formula microorganism inoculation marking off gathers and cultivates external member
CN113969237B (en) * 2021-10-19 2024-04-26 微尔创(武汉)医疗科技有限公司 Totally-enclosed microorganism inoculation streaking acquisition and culture kit

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C14 Grant of patent or utility model
GR01 Patent grant
C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20160224

Address after: 426100 Hunan province Changsha Panpan Changsha economic and Technological Development Zone, Road No. 9

Patentee after: Hunan numerous mountains Medical Devices Co., Ltd.

Address before: Hongkong Chinese Xiangli Chai Wan Industrial Zone No. 9 Street Xiangli Arts crafts emporium 7B

Patentee before: Hua Shan Microbiological Technology Limited

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20141217

Termination date: 20200822