CN108587885A - A kind of micro-biological samples streak inoculation device and method - Google Patents
A kind of micro-biological samples streak inoculation device and method Download PDFInfo
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- CN108587885A CN108587885A CN201810369289.2A CN201810369289A CN108587885A CN 108587885 A CN108587885 A CN 108587885A CN 201810369289 A CN201810369289 A CN 201810369289A CN 108587885 A CN108587885 A CN 108587885A
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- bacterium solution
- injection
- micro
- biological samples
- guiding hole
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
Abstract
The present invention relates to micro-biological samples processing technology fields, specifically a kind of micro-biological samples streak inoculation device and method, including culture dish, ware lid and bacterium solution injector, the bacterium solution injector includes injection pipe, injection set, spring and bacterium solution holding bottle, the injection is put on equipped with the long guiding hole along its width direction, sealing shroud is equipped at the long guiding hole, the injection wore sealing shroud and long guiding hole and extended to inside culture dish and ware lid, and the injection set can be slided along the length direction of long guiding hole；The inner wall top surface of the ware lid is equipped with scribing line guide groove, and the injection pipe is equipped with and the scribing line matched sliding block of guide groove.Compared with the existing technology, micro-biological samples streak inoculation device and method of the present invention has the beneficial effect that：The operating procedure of streak inoculation is simple, and streak inoculation is efficient, and fault rate is low, effectively miscellaneous bacteria can be avoided to enter, and the effect of inoculation separation bacterium colony is good.
Description
Technical field
The present invention relates to micro-biological samples processing technology field, specifically a kind of micro-biological samples streak inoculation device and side
Method.
Background technology
Plate streaking inoculation refers to the different cells in microorganism mixed in together or same micropopulation with connecing
Kind ring obtains the more individual cells being independently distributed on plating medium surface by sectional streak dilution, is grown after culture
It is multiplied into single bacterium colony, usually this single bacterium colony as the purebred of microorganism to be separated.The method of existing plate streaking inoculation
Including：A, it marks, in culture dish bottom surface, the bacterium name being inoculated with, inoculator's name, date etc. is indicated with marking pen；B, disinfection inoculation
Ring lights alcolhol burner, and the right hand holds oese, and places incineration in flame；C, strain, left hand is taken to hold the examination equipped with bacterium solution
Pipe is chosen test tube plug with the right hand palm and little finger of toe for holding oese, and test tube nozzle, which is run through flame, to go out for 2 ~ 3 times
Bacterium stretches into oese after cooling in test tube, takes the bacterium solution of an oese, is then log out test tube, again by strain tube nozzle
By 2 ~ 3 sterilizings of flame, it is stoppered test tube plug, puts back to original position；D, streak inoculation controls ware lid with left hand thumb and index finger, left
A few finger control wares of remaining of hand bottom, opens ware lid, angular aperture is made to be less than 30 °, in case the bacterium in air falls into wherein, and it is close
Flame, the right hand hold oese and back and forth cross on culture medium, paint a bacterium film.Existing plate streaking inoculation method exists
Following disadvantage：（1）In order to prevent miscellaneous bacteria from entering culture dish as far as possible, needs to sterilize to oese and strain test tube calcination, draw
Line needs to control ware lid with left hand thumb and index finger when being inoculated with, remaining several fingers control wares bottom of left hand opens ware lid, makes angular aperture
Less than 30 °, and close to flame, sufficiently complex, inefficiency is operated, is easy to accidentally drop and culture dish is caused to damage, miscellaneous bacteria is easy beating
Enter culture dish when opening ware lid；（2）It needs repeatedly to take strain, it is not only cumbersome, and be easy to cause miscellaneous bacteria and enter culture dish；
（3）The bacterium solution volume taken out every time differs, and cannot make effectively to desalinate separation of bacterial after scribing line, influences the effect of inoculation separation bacterium colony
Fruit.
Application No. is 201420476186.3 utility model patents to disclose a kind of micro-biological samples streak inoculation device,
When in use, culture plate state in a vertical shape, the bacterium solution in liquefier cup flow into culture dish bottom through liquefier cup cannula bevel channel
The sample diversion trench of seat, makes streak inoculation end be soaked in the sample of sample diversion trench, and streak inoculation pen is in scribing line machinery
The streak inoculation on culture medium under the drive of hand.The micro-biological samples streak inoculation device need not open culture plate in inoculation
Lid can effectively place miscellaneous bacteria entrance, need not also use high-temperature sterilization, easy to operate, efficient, still, the micro-biological samples
Streak inoculation device has the following disadvantages：（1）Culture plate is placed vertically, and bacterium solution can be converged under gravity in drop after scribing line
Shape can not effectively desalinate separation of bacterial, influence the effect of inoculation separation bacterium colony；（2）Bacterium solution on streak inoculation pen is with scribing line
Inoculation can be fewer and fewer, causes the bacterium solution film that scribing line is formed uneven.
Invention content
The purpose of the present invention is to solve above-mentioned the deficiencies in the prior art, provide a kind of micro-biological samples streak inoculation dress
It sets, streak inoculation process need not open ware lid, and scratching process carries out inside the culture dish of relative closure, to simplify operation
Step improves streak inoculation efficiency, reduces fault rate, effectively miscellaneous bacteria is avoided to enter, in addition, bacterium solution can uniformly enter culture
Ware simultaneously forms uniform bacterium solution film, to effectively desalinate separation of bacterial, improves the effect of inoculation separation bacterium colony.
The technical problems to be solved by the invention are realized using following technical scheme：A kind of micro-biological samples streak inoculation
Device, including culture dish, ware lid and bacterium solution injector, the ware lid are fastened on culture dish；The bacterium solution injector includes note
Enter pipe, injection set, spring and bacterium solution holding bottle；The injection pipe is slidably set in injection set inside, the injection pipe position
One end inside injection set is equipped with piston, and the spring is located between injection set and piston；The end of the injection set is equipped with
Holding bottle connector, the holding bottle connector are respectively equipped with matched internal thread and external screw thread with bacterium solution holding bottle, and the bacterium solution is deposited
The bottle mouth position of storage bottle is equipped with sealer, and puncture portion is equipped in the holding bottle connector；The injection is put on equipped with along its width direction
Long guiding hole, sealing shroud is equipped at the long guiding hole, one end and the ware lid of the sealing shroud are tightly connected, the sealing shroud
Including rigid portion and flexible part, the rigid portion is located at the both ends of long guiding hole, and the flexible part is located at guiding length
Above and below hole, the both ends of the flexible part are connect with two rigid portions respectively, and matching is respectively equipped on two panels flexible part
Recessed bone sealing strip and convex bone sealing strip；The injection wore sealing shroud and long guiding hole and extended in culture dish and ware lid
Portion, injection set can slides along the length direction of long guiding hole, the both sides of the injection set be respectively provided with one group be used to clamp it is recessed
The clamp arm of bone sealing strip and convex bone sealing strip；The inner wall top surface of the ware lid is equipped with scribing line guide groove, is set on the injection pipe
Have and the scribing line matched sliding block of guide groove.
Technical scheme of the present invention also has：The scribing line guide groove includes rake and turning part, two neighboring rake
End connected by turning part.
Technical scheme of the present invention also has：The injection pipe is located at one end inside culture dish and ware lid and is equipped with bending part,
The outlet of the bending part is towards the inner wall bottom surface of culture dish.
Technical scheme of the present invention also has：The end of the bending part is equipped with horn mouth, can improve the effect for shakeouing bacterium solution
Fruit.
The present invention also provides a kind of micro-biological samples streak inoculation methods, include the following steps：
A, culture medium is inserted in culture dish, to micro-biological samples streak inoculation device high temperature sterilization, is indicated and is connect on culture dish
Bacterium name, inoculator's name, date for planting etc.；
B, bacterium solution holding bottle is connect with holding bottle joint screw thread, with the relative movement of bacterium solution holding bottle and holding bottle connector,
Sealer is pierced through in puncture portion, and bacterium solution flows into injection set；
C, bacterium solution injector is made to reciprocatingly slide along long guiding hole, the recessed bone sealing strip and convex bone sealing strip of injection set both sides are clamping
Engaging sealing, injection pipe axial time of opposite injection set under the action of scribing line guide groove, sliding block and spring are kept under the action of arm
Contracting, the track movement for exporting guide groove to the layout line of injection pipe, bacterium solution are uniformly flowed out from injection pipe under the extruding of piston,
Bacterium solution is uniformly applied to formation bacterium solution film on culture medium by injection pipe.
Compared with the existing technology, micro-biological samples streak inoculation device of the present invention has the beneficial effect that：（1）Streak inoculation
Journey need not open ware lid, and for injection set along during long guiding hole slides, the recessed bone sealing strip and convex bone of injection set both sides are close
Strip of paper used for sealing keeps engaging to seal under the action of clamp arm, and streak inoculation process is made to be carried out inside the culture dish of relative closure, only
Need to make bacterium solution injector along long guiding hole reciprocatingly slide can streak inoculation, operating procedure is simple, and streak inoculation is efficient, error
Rate is low, effectively miscellaneous bacteria can be avoided to enter；（2）During streak inoculation, bacterium solution is uniformly squeezed into culture dish and shape
At uniform bacterium solution film, kind need not be repeatedly taken, inoculation efficiency is high, and the effect of inoculation separation bacterium colony is good.
Description of the drawings
Fig. 1 is the stereogram of micro-biological samples streak inoculation device of the present invention.
Fig. 2 is the partial enlarged view in the portions A in Fig. 1.
Fig. 3 is the sectional view of micro-biological samples streak inoculation device of the present invention.
Fig. 4 is the partial enlarged view in the portions B in Fig. 3.
Fig. 5 be Fig. 3 in C to sectional view.
Fig. 6 is the structural schematic diagram of ware lid of the present invention.
Fig. 7 is the structural schematic diagram of bacterium solution injector of the present invention.
In figure：1, culture dish, 2, ware lid, 3, injection pipe, 4, injection set, 5, spring, 6, bacterium solution holding bottle, 7, piston, 8,
Holding bottle connector, 9, puncture portion, 10, long guiding hole, 11, rigid portion, 12, flexible part, 13, recessed bone sealing strip, 14, the sealing of convex bone
Item, 15, clamp arm, 16, sliding block, 17, rake, 18, turning part, 19, bending part, 20, horn mouth.
Specific implementation mode
In order to clarify the technical characteristics of the invention, making to the specific embodiment of the invention below according to attached drawing further
Explanation.
As shown in Fig. 1 ~ Fig. 7, a kind of micro-biological samples streak inoculation device, including culture dish 1, ware lid 2 and bacterium solution injection
Device.Ware lid 2 is fastened on culture dish 1, and culture dish 1 and ware lid 2 are all made of transparent material and are made, and organic glass is used in the present embodiment
Glass.
As shown in Figure 3 and Figure 7, bacterium solution injector includes injection pipe 3, injection set 4, spring 5 and bacterium solution holding bottle 6.Injection
Pipe 3 is slidably set in inside injection set 4, and one end that injection pipe 3 is located inside injection set 4 is equipped with piston 7, and spring 5 is located at
Between injection set 4 and piston 7.
The end of injection set 4 is equipped with holding bottle connector 8, and holding bottle connector 8 is respectively equipped with bacterium solution holding bottle 6 in matched
Screw thread and external screw thread, bacterium solution holding bottle 6 is transparent material and is equipped with scale, to observe bacterium solution injection rate.Bacterium solution holding bottle 6
Bottle mouth position is equipped with sealer, and the puncture portion 9 for puncturing sealer is equipped in holding bottle connector 8.
Injection set 4 is equipped with the long guiding hole 10 along its width direction, and sealing shroud is equipped at long guiding hole 10, sealing shroud
One end is tightly connected with ware lid 2.As shown in Figure 1, Figure 2, shown in Fig. 4 and Fig. 5, sealing shroud includes rigid portion 11 and flexible part 12, rigidity
Portion 11 is located at the both ends of long guiding hole 10, and flexible part 12 is located above and below long guiding hole 10, flexible part 12
Both ends connect respectively with two rigid portions 11, matched recessed bone sealing strip 13 is respectively equipped on two panels flexible part 12 and convex bone is close
Strip of paper used for sealing 14.Injection set 4 passes through sealing shroud and long guiding hole 10 and extends to inside culture dish 1 and ware lid 2, and injection set 4 can edge
The length direction of long guiding hole 10 slides, and the both sides of injection set 4 are respectively provided with one group for clamping recessed bone sealing strip 13 and convex bone is close
The clamp arm 15 of strip of paper used for sealing 14.
As shown in fig. 6, the inner wall top surface of ware lid 2 is equipped with scribing line guide groove, scribing line guide groove includes rake 17 and turns to
The end in portion 18, two neighboring rake 17 is connected by turning part 18.Injection pipe 3 is equipped with and the scribing line matched cunning of guide groove
Block 16.
As shown in figure 3, injection pipe 3, which is located at one end inside culture dish 1 and ware lid 2, is equipped with bending part 19, bending part 19
End is equipped with horn mouth 20, and horn mouth 20 is towards the inner wall bottom surface of culture dish 1.
The present invention also provides a kind of methods of micro-biological samples streak inoculation, include the following steps：
A, culture medium is inserted in culture dish 1, to the micro-biological samples streak inoculation device high temperature sterilization of the present invention, in culture dish
The bacterium name being inoculated with, inoculator's name, date etc. are indicated on 1；
B, bacterium solution holding bottle 6 is threadedly coupled with holding bottle connector 8, as bacterium solution holding bottle 6 and the opposite of holding bottle connector 8 move
Dynamic, sealer is pierced through in puncture portion 9, and bacterium solution flows into injection set 4；
C, bacterium solution injector is made to reciprocatingly slide along long guiding hole 10, the recessed bone sealing strip 13 and convex bone sealing strip of 4 both sides of injection set
14 keep engaging sealing, phase under the action of crossing guide groove, sliding block 16 and spring 5 of injection pipe 3 under the action of clamp arm 15
To injection cover 4 axial retractions, horn mouth 20 to the layout line guide groove track movement, bacterium solution under the extruding of piston 7 uniformly from
Horn mouth 20 flows out, and bacterium solution is uniformly applied to formation bacterium solution film on culture medium by horn mouth 20.
The embodiment of the present invention is elaborated above in conjunction with attached drawing, but the present invention is not limited to above-mentioned implementations
Example, within the knowledge of a person skilled in the art, can also do without departing from the purpose of the present invention
Go out various change.
Claims (5)
1. a kind of micro-biological samples streak inoculation device, including culture dish（1）And ware lid（2）, the ware lid（2）It is fastened on culture
Ware（1）On, it is characterised in that：Further include bacterium solution injector, the bacterium solution injector includes injection pipe（3）, injection set（4）, bullet
Spring（5）With bacterium solution holding bottle（6）；The injection pipe（3）It is slidable to be set in injection set（4）Inside, the injection pipe（3）Position
It is covered in injection（4）Internal one end is equipped with piston（7）, the spring（5）It is located at injection set（4）With piston（7）Between；The note
Enter set（4）End be equipped with holding bottle connector（8）, the holding bottle connector（8）With bacterium solution holding bottle（6）It is respectively equipped with matched
Internal thread and external screw thread, the bacterium solution holding bottle（6）Bottle mouth position be equipped with sealer, the holding bottle connector（8）Interior be equipped with punctures
Portion（9）；The injection set（4）It is equipped with the long guiding hole along its width direction（10）, the long guiding hole（10）Place is equipped with close
Big envelope, one end of the sealing shroud and ware lid（2）It is tightly connected, the sealing shroud includes rigid portion（11）And flexible part（12）,
The rigid portion（11）It is located at long guiding hole（10）Both ends, the flexible part（12）It is located at long guiding hole（10）'s
Above and below, the flexible part（12）Both ends respectively with two rigid portions（11）Connection, two panels flexible part（12）Upper difference
Equipped with matched recessed bone sealing strip（13）With convex bone sealing strip（14）；The injection set（4）Across sealing shroud and long guiding hole
（10）And and extend to culture dish（1）And ware lid（2）Inside, the injection set（4）It can be along long guiding hole（10）Length direction
Sliding, the injection set（4）Both sides be respectively provided with one group for clamping recessed bone sealing strip（13）With convex bone sealing strip（14）Folder
Tight arm（15）；The ware lid（2）Inner wall top surface be equipped with scribing line guide groove, the injection pipe（3）It is equipped with and scribing line guide groove
Matched sliding block（16）.
2. micro-biological samples streak inoculation device according to claim 1, it is characterised in that：The scribing line guide groove includes
Rake（17）The turning part and（18）, two neighboring rake（17）End pass through turning part（18）Connection.
3. micro-biological samples streak inoculation device according to claim 1, it is characterised in that：The injection pipe（3）It is located at
Culture dish（1）And ware lid（2）Internal one end is equipped with bending part（19）, the bending part（19）Outlet towards culture dish（1）
Inner wall bottom surface.
4. micro-biological samples streak inoculation device according to claim 3, it is characterised in that：The bending part（19）End
Portion is equipped with horn mouth（20）.
5. a kind of micro-biological samples streak inoculation device using as described in claim 1 ~ 9 carries out micro-biological samples streak inoculation
Method, include the following steps：
A, in culture dish（1）Middle filling culture medium, to micro-biological samples streak inoculation device high temperature sterilization, in culture dish（1）On
Indicate the bacterium name being inoculated with, inoculator's name, date etc.；
B, by bacterium solution holding bottle（6）With holding bottle connector（8）It is threadedly coupled, with bacterium solution holding bottle（6）With holding bottle connector（8）
Relative movement, puncture portion（9）Sealer is pierced through, bacterium solution flows into injection set（4）It is interior；
C, make bacterium solution injector along long guiding hole（10）It reciprocatingly slides, injection set（4）The recessed bone sealing strip of both sides（13）With convex bone
Sealing strip（14）In clamp arm（15）Under the action of keep engaging sealing, injection pipe（3）In scribing line guide groove, sliding block（16）And bullet
Spring（5）Under the action of opposite injection set（4）Axial retraction, injection pipe（3）Outlet to the layout line guide groove track movement, bacterium
Liquid is in piston（7）Extruding under uniformly from injection pipe（3）Middle outflow, injection pipe（3）Bacterium solution is uniformly applied on culture medium
Form bacterium solution film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369289.2A CN108587885B (en) | 2018-04-23 | 2018-04-23 | A kind of micro-biological samples streak inoculation device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369289.2A CN108587885B (en) | 2018-04-23 | 2018-04-23 | A kind of micro-biological samples streak inoculation device and method |
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| Publication Number | Publication Date |
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| CN108587885A true CN108587885A (en) | 2018-09-28 |
| CN108587885B CN108587885B (en) | 2019-03-01 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110885745A (en) * | 2019-11-27 | 2020-03-17 | 邳州高新区生物医药研究院有限公司 | Inoculation culture dish for biological medicine |
| CN110982679A (en) * | 2020-01-08 | 2020-04-10 | 台州黄岩博友智能技术有限公司 | Inoculation device for microbiological assay |
| CN113980791A (en) * | 2021-11-30 | 2022-01-28 | 深圳零一生命科技有限责任公司 | Automatic microorganism inoculation instrument and inoculation scribing method thereof |
| CN114456919A (en) * | 2022-03-17 | 2022-05-10 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment marine microorganism solid separation culture device and culture method |
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