CN208234905U - A kind of micro-biological samples streak inoculation device - Google Patents
A kind of micro-biological samples streak inoculation device Download PDFInfo
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- CN208234905U CN208234905U CN201820582275.4U CN201820582275U CN208234905U CN 208234905 U CN208234905 U CN 208234905U CN 201820582275 U CN201820582275 U CN 201820582275U CN 208234905 U CN208234905 U CN 208234905U
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- injection
- guiding hole
- bacterium solution
- long guiding
- ware lid
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Abstract
The utility model relates to micro-biological samples processing technology fields, specifically a kind of micro-biological samples streak inoculation device, including culture dish, ware lid and bacterium solution injector, the bacterium solution injector includes injection pipe, injection set, spring and bacterium solution holding bottle, the injection puts on the long guiding hole being equipped with along its width direction, sealing shroud is equipped at the long guiding hole, the injection wore sealing shroud and long guiding hole and extended to inside culture dish and ware lid, and the injection set can be slided along the length direction of long guiding hole；The inner wall top surface of the ware lid is equipped with scribing line guide groove, and the injection pipe is equipped with and the scribing line matched sliding block of guide groove.Compared with the existing technology, the utility model micro-biological samples streak inoculation device has the beneficial effect that the operating procedure of streak inoculation is simple, and streak inoculation is high-efficient, and fault rate is low, effectively miscellaneous bacteria can be avoided to enter, and the effect of inoculation separation bacterium colony is good.
Description
Technical field
The utility model relates to micro-biological samples processing technology field, specifically a kind of micro-biological samples streak inoculation dress
It sets.
Background technique
Plate streaking inoculation refers to the different cells in microorganism mixed in together or same micropopulation with connecing
Kind ring obtains the more individual cells being independently distributed by sectional streak dilution on plating medium surface, grows after cultivation
It is multiplied into single colonie, usually this single colonie as the purebred of microorganism to be separated.The method of existing plate streaking inoculation
Include: a, label, in culture dish bottom surface, indicates the bacterium name being inoculated with, inoculator's name, date etc. with marking pen；B, disinfection inoculation
Ring lights alcolhol burner, and the right hand holds oese, and places incineration in flame；C, strain is taken, left hand holds the examination equipped with bacterium solution
Pipe is chosen test tube plug with the right hand palm and little finger of toe for holding oese, test tube nozzle is run through flame 2 ~ 3 times and is gone out
Bacterium protrudes into oese after cooling in test tube, takes the bacterium solution of an oese, is then log out test tube, again by strain tube nozzle
It is sterilized by flame 2 ~ 3 times, is stoppered test tube plug, puts back to original position；D, streak inoculation controls ware lid with left hand thumb and index finger, left
Several fingers of remaining of hand control ware bottom, open ware lid, make angular aperture less than 30 °, in case the bacterium in air falls into wherein, and close
Flame, the right hand hold oese and back and forth cross on culture medium, paint a bacterium film.Existing plate streaking inoculation method exists
Following disadvantage: (1) it in order to as far as possible prevent miscellaneous bacteria from entering culture dish, needs to sterilize to oese and strain test tube calcination, draw
Line needs to control ware lid with left hand thumb and index finger when being inoculated with, and several fingers of remaining of left hand control ware bottom, open ware lid, make angular aperture
Less than 30 °, and close to flame, sufficiently complex, inefficiency is operated, is easy to accidentally drop and culture dish is caused to damage, miscellaneous bacteria is easy beating
Enter culture dish when opening ware lid；(2) it needs repeatedly to take strain, it is not only cumbersome, and miscellaneous bacteria is easy to cause to enter culture dish；
(3) bacterium solution volume taken out every time differs, and cannot make effectively to desalinate separation of bacterial after scribing line, influences the effect of inoculation separation bacterium colony
Fruit.
Application No. is 201420476186.3 utility model patents to disclose a kind of micro-biological samples streak inoculation device,
When in use, culture plate state in a vertical shape, the bacterium solution in liquefier cup flow into culture dish bottom through liquefier cup cannula bevel channel
The sample diversion trench of seat, is soaked in streak inoculation end in the sample of sample diversion trench, and streak inoculation pen is mechanical in scribing line
The streak inoculation on culture medium under the drive of hand.The micro-biological samples streak inoculation device does not need to open culture plate in inoculation
Lid can effectively place miscellaneous bacteria entrance, also not need using high-temperature sterilization, easy to operate, high-efficient, still, the micro-biological samples
Streak inoculation device has the disadvantage in that (1) culture plate is placed vertically, and bacterium solution can be converged under gravity in drop after scribing line
Shape can not effectively desalinate separation of bacterial, influence the effect of inoculation separation bacterium colony；(2) bacterium solution on streak inoculation pen is with scribing line
Inoculation can be fewer and fewer, and the bacterium solution film for causing scribing line to be formed is uneven.
Utility model content
Purpose of the utility model is to solve above-mentioned the deficiencies in the prior art, a kind of micro-biological samples scribing line is provided and is connect
Kind device, streak inoculation process do not need to open ware lid, and scratching process carries out inside the culture dish of relative closure, to simplify
Operating procedure improves streak inoculation efficiency, reduces fault rate, effectively miscellaneous bacteria is avoided to enter, in addition, bacterium solution can uniformly enter
Culture dish simultaneously forms uniform bacterium solution film, to effectively desalinate separation of bacterial, improves the effect of inoculation separation bacterium colony.
Technical problem to be solved in the utility model is realized using following technical scheme: a kind of micro-biological samples scribing line
Classification inoculation apparatus, including culture dish, ware lid and bacterium solution injector, the ware lid are fastened on culture dish；The bacterium solution injector packet
Include injection pipe, injection set, spring and bacterium solution holding bottle；The injection pipe is slidably set in injection set inside, the injection
One end that pipe is located inside injection set is equipped with piston, and the spring is located between injection set and piston；The injection pipe is located at training
Support one end inside ware and ware lid and be equipped with bending part, the end of the bending part is equipped with hairbrush, the hairbrush and culture dish it is interior
The contact of wall bottom surface；The end of the injection set is equipped with holding bottle connector, and the holding bottle connector is respectively equipped with bacterium solution holding bottle
Matched internal screw thread and external screw thread, the bottle mouth position of the bacterium solution holding bottle are equipped with sealer, are equipped with and puncture in the holding bottle connector
Portion；The injection puts on the long guiding hole being equipped with along its width direction, is equipped with sealing shroud, the sealing shroud at the long guiding hole
One end and ware lid be tightly connected, the sealing shroud includes rigid portion and flexible part, and the rigid portion is located at long guiding hole
Both ends, the flexible part is located above and below long guiding hole, the both ends of the flexible part respectively with two rigidity
Portion connects, and is respectively equipped with matched recessed bone sealing strip and convex bone sealing strip on two panels flexible part；The injection wore sealing shroud
With long guiding hole and extend to inside culture dish and ware lid, the injection set can be slided along the length direction of long guiding hole, described
The two sides of injection set are respectively provided with one group for clamping the clamp arm of recessed bone sealing strip and convex bone sealing strip；The inner wall top of the ware lid
Face is equipped with scribing line guide groove, and the injection pipe is equipped with and the scribing line matched sliding block of guide groove.
The technical solution of the utility model is also: the scribing line guide groove includes rake and steering portion, two neighboring to incline
The end of inclined portion is connected by steering portion.
The utility model additionally provides a kind of micro-biological samples streak inoculation method, comprising the following steps:
A, culture medium is inserted in culture dish, to micro-biological samples streak inoculation device high temperature sterilization, is infused on culture dish
The bacterium name of bright inoculation, inoculator's name, date etc.；
B, bacterium solution holding bottle is connect with holding bottle joint screw thread, as bacterium solution holding bottle and the opposite of holding bottle connector move
Dynamic, sealer is pierced through in puncture portion, and bacterium solution flows into injection set；
C, bacterium solution injector is made to reciprocatingly slide along long guiding hole, the recessed bone sealing strip and convex bone sealing strip of injection set two sides exist
Engaging sealing, injection pipe opposite injection set axis under the action of crossing guide groove, sliding block and spring are kept under the action of clamp arm
To retraction, the track for exporting guide groove to the layout line of injection pipe is mobile, and bacterium solution is under the extruding of piston uniformly from injection pipe
Bacterium solution is uniformly applied to formation bacterium solution film on culture medium by outflow, injection pipe.
Compared with the existing technology, the having the beneficial effect that of the utility model micro-biological samples streak inoculation device (1) scribing line connects
Kind of process does not need to open ware lid, and injection set is during the long guiding hole sliding, the recessed bone sealing strip of injection set two sides and convex
Bone sealing strip kept under the action of clamp arm engaging seal, make streak inoculation process inside the culture dish of relative closure into
Row, only need to make bacterium solution injector along long guiding hole reciprocatingly slide can streak inoculation, operating procedure is simple, streak inoculation efficiency
Height, fault rate is low, effectively miscellaneous bacteria can be avoided to enter；(2) during streak inoculation, bacterium solution is uniformly squeezed into culture
Ware simultaneously forms uniform bacterium solution film, does not need repeatedly to take kind, and inoculation efficiency is high, and the effect of inoculation separation bacterium colony is good.
Detailed description of the invention
Fig. 1 is the perspective view of the utility model micro-biological samples streak inoculation device.
Fig. 2 is the partial enlarged view in the portion A in Fig. 1.
Fig. 3 is the cross-sectional view of the utility model micro-biological samples streak inoculation device.
Fig. 4 is the partial enlarged view in the portion B in Fig. 3.
Fig. 5 be Fig. 3 in C to cross-sectional view.
Fig. 6 is the structural schematic diagram of the utility model ware lid.
Fig. 7 is the structural schematic diagram of the utility model bacterium solution injector.
In figure: 1, culture dish, 2, ware lid, 3, injection pipe, 4, injection set, 5, spring, 6, bacterium solution holding bottle, 7, piston, 8,
Holding bottle connector, 9, puncture portion, 10, long guiding hole, 11, rigid portion, 12, flexible part, 13, recessed bone sealing strip, 14, the sealing of convex bone
Item, 15, clamp arm, 16, sliding block, 17, rake, 18, steering portion, 19, bending part, 20, hairbrush.
Specific embodiment
In order to clarify the technical characteristics of the invention, below according to attached drawing to specific embodiment of the present invention make into
One step explanation.
As shown in Fig. 1 ~ Fig. 7, a kind of micro-biological samples streak inoculation device, including culture dish 1, ware lid 2 and bacterium solution injection
Device.Ware lid 2 is fastened on culture dish 1, and culture dish 1 and ware lid 2 are all made of transparent material and are made, and organic glass is used in the present embodiment
Glass.
As shown in Figure 3 and Figure 7, bacterium solution injector includes injection pipe 3, injection set 4, spring 5 and bacterium solution holding bottle 6.Injection
Pipe 3 is slidably set in inside injection set 4, and one end that injection pipe 3 is located inside injection set 4 is equipped with piston 7, and spring 5 is located at
Between injection set 4 and piston 7.
The end of injection set 4 is equipped with holding bottle connector 8, and holding bottle connector 8 and bacterium solution holding bottle 6 are respectively equipped in matched
Screw thread and external screw thread, bacterium solution holding bottle 6 is transparent material and is equipped with scale, to observe bacterium solution injection rate.Bacterium solution holding bottle 6
Bottle mouth position is equipped with sealer, and the puncture portion 9 for puncturing sealer is equipped in holding bottle connector 8.
Injection set 4 is equipped with the long guiding hole 10 along its width direction, is equipped with sealing shroud at long guiding hole 10, sealing shroud
One end and ware lid 2 are tightly connected.As shown in Figure 1, Figure 2, shown in Fig. 4 and Fig. 5, sealing shroud includes rigid portion 11 and flexible part 12, rigidity
Portion 11 is located at the both ends of long guiding hole 10, and flexible part 12 is located above and below long guiding hole 10, flexible part 12
Both ends connect respectively with two rigid portions 11, matched recessed bone sealing strip 13 is respectively equipped on two panels flexible part 12 and convex bone is close
Strip of paper used for sealing 14.Injection set 4 passes through sealing shroud and long guiding hole 10 and extends to inside culture dish 1 and ware lid 2, and injection set 4 can edge
The length direction of long guiding hole 10 slides, and the two sides of injection set 4 are respectively provided with one group for clamping recessed bone sealing strip 13 and convex bone is close
The clamp arm 15 of strip of paper used for sealing 14.
As shown in fig. 6, the inner wall top surface of ware lid 2 is equipped with scribing line guide groove, scribing line guide groove includes rake 17 and turns to
The end in portion 18, two neighboring rake 17 is connected by steering portion 18.Injection pipe 3 is equipped with and the scribing line matched cunning of guide groove
Block 16.
As shown in figure 3, injection pipe 3, which is located at one end inside culture dish 1 and ware lid 2, is equipped with bending part 19, bending part 19
End is equipped with hairbrush 20, and hairbrush 20 is contacted with the inner wall bottom surface of culture dish 1.
The utility model additionally provides a kind of method of micro-biological samples streak inoculation, comprising the following steps:
A, culture medium is inserted in culture dish 1, to the micro-biological samples streak inoculation device high temperature sterilization of the utility model,
The bacterium name being inoculated with, inoculator's name, date etc. are indicated on culture dish 1；
B, bacterium solution holding bottle 6 is threadedly coupled with holding bottle connector 8, with the phase of bacterium solution holding bottle 6 and holding bottle connector 8
To movement, sealer is pierced through in puncture portion 9, and bacterium solution flows into injection set 4；
C, bacterium solution injector is made to reciprocatingly slide along long guiding hole 10, the recessed bone sealing strip 13 and convex bone of 4 two sides of injection set are close
Strip of paper used for sealing 14 keeps engaging to seal under the action of clamp arm 15, effect of the injection pipe 3 in scribing line guide groove, sliding block 16 and spring 5
The track of 4 axial retractions of lower opposite injection set, the guide groove to the layout line of hairbrush 20 is mobile, bacterium solution under the extruding of piston 7 uniformly
It is flowed out from hairbrush 20, bacterium solution is uniformly applied to formation bacterium solution film on culture medium by hairbrush 20.
The embodiments of the present invention are elaborated above in conjunction with attached drawing, but the utility model is not limited to
Embodiment is stated, within the knowledge of a person skilled in the art, the utility model aims can also not departed from
Under the premise of make a variety of changes.
Claims (2)
1. a kind of micro-biological samples streak inoculation device, including culture dish (1) and ware lid (2), the ware lid (2) are fastened on culture
On ware (1), it is characterised in that: further include bacterium solution injector, the bacterium solution injector includes injection pipe (3), injection set (4), bullet
Spring (5) and bacterium solution holding bottle (6)；The injection pipe (3) is slidably set in injection set (4) inside, injection pipe (3) position
Piston (7) are equipped in the internal one end of injection set (4), the spring (5) is located between injection set (4) and piston (7)；The note
Enter one end that pipe (3) is located inside culture dish (1) and ware lid (2) and be equipped with bending part (19), the end of the bending part (19) is set
Have hairbrush (20), the hairbrush (20) contacts with the inner wall bottom surface of culture dish (1)；The end of the injection set (4) is equipped with storage
Bottle connector (8), the holding bottle connector (8) and bacterium solution holding bottle (6) are respectively equipped with matched internal screw thread and external screw thread, the bacterium
The bottle mouth position of liquid holding bottle (6) is equipped with sealer, is equipped with puncture portion (9) in the holding bottle connector (8)；On the injection set (4)
Equipped with the long guiding hole (10) along its width direction, sealing shroud, one end of the sealing shroud are equipped at the long guiding hole (10)
It is tightly connected with ware lid (2), the sealing shroud includes rigid portion (11) and flexible part (12), and the rigid portion (11) is located at
The both ends of long guiding hole (10), the flexible part (12) are located above and below long guiding hole (10), the flexible part
(12) both ends are connect with two rigid portions (11) respectively, and matched recessed bone sealing strip is respectively equipped on two panels flexible part (12)
(13) and convex bone sealing strip (14)；Injection set (4) pass through sealing shroud and long guiding hole (10) and extend to culture dish (1) and
Ware lid (2) is internal, and the injection set (4) can be slided along the length direction of long guiding hole (10), and the two sides of the injection set (4) are each
Equipped with one group for clamping the clamp arm (15) of recessed bone sealing strip (13) and convex bone sealing strip (14)；The inner wall of the ware lid (2)
Top surface is equipped with scribing line guide groove, and the injection pipe (3) is equipped with and the scribing line matched sliding block of guide groove (16).
2. micro-biological samples streak inoculation device according to claim 1, it is characterised in that: the scribing line guide groove includes
The end of rake (17) and steering portion (18), two neighboring rake (17) is connected by steering portion (18).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820582275.4U CN208234905U (en) | 2018-04-23 | 2018-04-23 | A kind of micro-biological samples streak inoculation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820582275.4U CN208234905U (en) | 2018-04-23 | 2018-04-23 | A kind of micro-biological samples streak inoculation device |
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| Publication Number | Publication Date |
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| CN208234905U true CN208234905U (en) | 2018-12-14 |
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| CN201820582275.4U Active CN208234905U (en) | 2018-04-23 | 2018-04-23 | A kind of micro-biological samples streak inoculation device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023173495A1 (en) * | 2022-03-17 | 2023-09-21 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment marine microorganism enrichment culture and gravity-type isolation apparatus |
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2018
- 2018-04-23 CN CN201820582275.4U patent/CN208234905U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023173495A1 (en) * | 2022-03-17 | 2023-09-21 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment marine microorganism enrichment culture and gravity-type isolation apparatus |
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