CN108680380B - Tissue slice device for plant quarantine - Google Patents
Tissue slice device for plant quarantine Download PDFInfo
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- CN108680380B CN108680380B CN201810275371.9A CN201810275371A CN108680380B CN 108680380 B CN108680380 B CN 108680380B CN 201810275371 A CN201810275371 A CN 201810275371A CN 108680380 B CN108680380 B CN 108680380B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
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Abstract
The invention provides a tissue slicing device for plant quarantine, which comprises a cutter and a second connecting pipe; the drawing groove is of a cavity groove structure, the bottom surface of the drawing groove is provided with a front supporting vertical plate and a rear supporting vertical plate which are of plate structures, and the bottom side of the drawing groove is also provided with a sampling box and a culture solution storage tank from left to right; a notch with a rectangular structure is formed in the position, close to the left side, of the rear wall of the inner cavity of the drawing groove; first connecting pipe is connected between culture solution storage jar and second connecting pipe, and the other end of second connecting pipe is connected in the funnel, use this device to carry out the sample work of stem and stem to the plant, it is more closed relatively, and can realize the synchronous relatively closed drip work of culture solution simultaneously, the section sampling device of more current plant quarantine usefulness, the operability is higher, and when the sample that has significantly reduced, with outside air's contact probability, the sampling process is more closed relatively, make follow-up sample utilize microscope observation time, the effect is better.
Description
Technical Field
The invention belongs to the technical field of slicing device structures, and particularly relates to a tissue slicing device for plant quarantine.
Background
The quarantine of plants adopts a legislative measure to prevent the plants and the products thereof from spreading harmful organisms in the circulation process. One aspect of plant protection is that it prevents the introduction, colonization and expansion of all harmful organisms, especially those not present in the area, macroscopically as a whole. Because of its legal obligation, "regulatory control" and "administrative action control" are often used as synonyms in the international literature.
Similar to chinese patent No.: CN201420599164.6, a plant quarantine sample bottle is provided, which is described as including a bottle body and a bottle cap, the bottle body includes a bottle neck, the bottle neck of the bottle body is connected with the bottle cap through a thread, a spring device is fixedly arranged at the center of the bottom inside the bottle body, a U-shaped handle is arranged at the upper part of the spring device, the width of the U-shaped handle is smaller than the diameter of the bottle neck, the height of the U-shaped handle is equal to the height of the bottle body minus the height of the spring device after compression, and a holding part is further arranged at the upper end of the U-.
And is similar to Chinese patent number: CN201420599116.7 provides a plant quarantine inspection tool cart, which comprises a cart body, wherein the cart body is provided with an operating platform, the lower part of the operating platform is provided with a tool box, the lower part of the tool box is provided with a power box, a storage battery is arranged in the power box, one side of the power box is provided with a garbage can, one end of the operating platform is also provided with a garbage hole, the garbage hole is communicated with the garbage can through a garbage channel, the other end of the operating platform is fixedly provided with a desk lamp, the desk lamp is electrically connected with the storage battery, four corners of the bottom end of,
based on the above, in the plant quarantine process, the plant needs to be sliced and sampled, then the sliced samples are placed under a microscope for observation, the change of the internal tissues is observed, and the final quarantine item is confirmed by matching with other chemical experimental data of the sampled samples, so that the purpose of quarantine is achieved. The sampling device similar to the above structures has a single structure and poor functionality, or has a large size, and during sampling, the cut plant stem section is easy to have a large contact chance with the outside air, so that when a sliced sample is observed under a subsequent microscope, the tissue structure has more other material tissues, and the viewing effect is influenced.
Disclosure of Invention
In order to solve the above technical problems and achieve the above design concept, the present invention provides a tissue slice apparatus for plant quarantine, which is achieved by the following specific technical means:
the tissue slicing device for plant quarantine comprises a supporting vertical plate, a drawing groove, a slide bar, a pulling plate, a funnel, a cutting cylinder, a corrugated puller, a threaded seat, a sampling box, a culture solution storage tank, a first connecting pipe, an overflow pipe, a pressure air bag pump, a cutter and a second connecting pipe; the drawing groove is of a cavity groove structure, the bottom surface of the drawing groove is provided with a front supporting vertical plate and a rear supporting vertical plate which are of plate structures, and the bottom side of the drawing groove is also provided with a sampling box and a culture solution storage tank from left to right; a notch with a rectangular structure is formed in the position, close to the left side, of the rear wall of the inner cavity of the drawing groove, and a cutter is arranged in the notch in a matched mode through a support shaft; the sampling box is communicated with the culture solution storage tank through an overflow pipe, and a pressure air bag pump is connected to the middle position of the overflow pipe; the right end of the pulling plate is provided with a T-shaped block structure for pulling, the left side of the top surface of the pulling plate is provided with a threaded seat with a circular ring structure, a cutting cylinder with a cylindrical cylinder structure is connected in the threaded seat through threads, and the top end of the cutting cylinder is also penetrated and matched with a corrugated pull head in a corrugated plastic pipe pattern; a funnel is further arranged at the right side of the top surface of the pulling plate; the first connecting pipe and the second connecting pipe are two connecting hoses connected on one pipeline, the first connecting pipe is connected between the culture solution storage tank and the second connecting pipe, and the other end of the second connecting pipe is connected to the funnel.
Furthermore, the funnel is communicated with the culture solution storage tank through a second connecting pipe penetrating through the rear side supporting vertical plate, and the pipeline routing track of the second connecting pipe is located at the bottom side of the pulling plate.
Furthermore, the junction of overflow pipe and culture solution storage jar is fixed, and the connected mode of the overflow pipe other end and sample box is activity through connection, and the overflow pipe other end is for running through the tubule of scalable activity in sample box promptly.
Furthermore, a circular through hole is formed in the contact position of the pulling plate and the threaded seat, the pulling plate is matched with the sliding strip in the drawing groove, and after the pulling plate moves leftwards on the sliding strip, the cutting cylinder on the top side of the pulling plate and the sampling box on the bottom side of the drawing groove are located on the same central axis.
Furthermore, the bottom surface of the drawing groove is also provided with a through hole of a circular structure, a thread is arranged in the through hole, the sampling box of the circular cylinder structure is matched with the circular structure on the bottom surface of the drawing groove through the thread, and the rotating circular cutting range of the cutter is positioned at the top side of the sampling box.
Furthermore, the corrugated pull head is of a laminated corrugated pipe structure, and the bottom end of the corrugated pull head is in threaded fit connection with the top end of the cutting cylinder.
Furthermore, the drawing groove is also provided with a penetrating hole structure for penetrating the second connecting pipe, the penetrating hole structure is in clearance fit with the second connecting pipe, and the second connecting pipe penetrates through the pulling plate with the same clearance fit hole and is communicated with the funnel.
Furthermore, the partial section of the overflow pipe extending into the sampling box and the middle pipeline adopt a backflow port design.
Compared with the prior art, the invention has the following beneficial effects:
this structure utilizes the core structure to take out the sample structure that groove and arm-tie cooperation formed, structural installation has ring cutting formula blade, it has the section of thick bamboo of cutting out of dismantled and assembled integrated configuration that is used for inserting plant stem to cooperate in the top side of arm-tie, draw groove bottom surface department at the arm-tie bottom side, run through the cooperation and have dismantled and assembled sampling box, utilize the sampling device that the relative more outside sample of these two structures is more sanitary to be done, plant stem is stretched into by a section of thick bamboo of cutting out of top side, utilize ring cutting blade ring cutting sample thin slice, make the thin slice fall on the sampling box of bottom, and still install the culture solution storage jar that is used for accessing the culture solution on the right side that the sample closed, make the plant stem thin slice after the closed sample, can realize the dropping liquid operation simultaneously, certain enclosed action has been played to culture solution bit process equally.
Above can find out, use this structure to carry out the sample work of stem and stem to the plant, relatively more sealed, and can realize the bit work of the synchronous relative seal nature of culture solution simultaneously, than the section sampling device that current plant quarantine was used, the operability is higher, and when having significantly reduced the sample, with the contact probability of outside air, the sampling process is more sealed relatively, makes follow-up sample utilize microscope observation time, and the effect is better.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a schematic diagram of an exploded structure of the present invention.
FIG. 3 is a schematic view of the bottom structure of the extraction groove of the present invention.
FIG. 4 is a schematic view of the bottom rear view of the extraction groove of the present invention.
Fig. 5 is a schematic top plan view of the present invention.
Fig. 6 is a schematic diagram of the right side plan view structure of the present invention.
Fig. 7 is an enlarged schematic view of the part a of the present invention from fig. 1.
FIG. 8 is a schematic diagram showing the fixed connection relationship between the overflow pipe of the present invention at the left side and the movable right side at the intermediate position between the sampling box and the culture storage tank.
FIG. 9 is a schematic view of the structure of the internal backflow port of the partial segment of the overflow pipe of the present invention extending into the left side sampling box.
In the figure: a supporting vertical plate-1, a drawing groove-2, a slide bar-3, a pulling plate-4, a funnel-5, a cutting cylinder-6, a corrugated pull head-7, a threaded seat-8, a sampling box-9, a culture fluid storage tank-10, a first connecting pipe-11, an overflow pipe-12, a pressure air bag pump-13, a cutter-14 and a second connecting pipe-15.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
example (b):
as shown in figures 1 to 9:
the invention provides a tissue slicing device for plant quarantine, which comprises a supporting vertical plate 1, a drawing groove 2, a slide bar 3, a pulling plate 4, a funnel 5, a cutting cylinder 6, a corrugated pull head 7, a threaded seat 8, a sampling box 9, a culture solution storage tank 10, a first connecting pipe 11, an overflow pipe 12, a pressure air bag pump 13, a cutter 14 and a second connecting pipe 15; the drawing groove 2 is of a cavity groove structure, the bottom surface of the drawing groove is provided with a supporting vertical plate 1 with a front plate-shaped structure and a rear plate-shaped structure, and the bottom side of the drawing groove 2 is also provided with a sampling box 9 and a culture solution storage tank 10 from left to right; a notch with a rectangular structure is formed in the position, close to the left side, of the rear wall of the inner cavity of the drawing groove 2, and a cutter 14 is arranged in the notch in a matched mode through a support shaft; the sampling box 9 is communicated with the culture solution storage tank 10 through an overflow pipe 12, and a pressure air bag pump 13 is connected to the middle position of the overflow pipe 12; the right end of the pulling plate 4 is provided with a T-shaped block structure for pulling, the left side of the top surface of the pulling plate 4 is provided with a threaded seat 8 with a circular ring structure, the threaded seat 8 is internally connected with a cutting cylinder 6 with a cylindrical cylinder structure through threads, and the top end of the cutting cylinder 6 is also penetrated and matched with a corrugated pull head 7 in a corrugated plastic pipe mode; a funnel 5 is further arranged at the right side of the top surface of the pulling plate 4; the first connection pipe 11 and the second connection pipe 15 are two connection hoses connected to each other on one pipe, the first connection pipe 11 is connected between the culture solution storage tank 10 and the second connection pipe 15, and the other end of the second connection pipe 15 is connected to the funnel 5
Wherein, funnel 5 is through running through second connecting pipe 15 and culture solution storage jar 10 on supporting riser 1 through the rear side and is connected mutually, and the pipeline of second connecting pipe 15 walks the line orbit and is located the bottom side position of arm-tie 4, prevents that arm-tie 4 from pulling the in-process, causes the damage to the pipeline, because arm-tie 4 is the structure that can pull open, the inner chamber of the whole structure of consequently being convenient for regularly pulls open the clearance, and the structure is more reasonable.
Wherein, the junction of overflow pipe 12 and culture solution storage jar 10 is fixed, and the connected mode of the overflow pipe 12 other end and sampling box 9 is activity through connection, and the overflow pipe 12 other end is for running through the thin pipe of scalable activity in sampling box 9 promptly, as shown in fig. 8, the activity can be realized to the partial pipe in left side, just so makes the dropping liquid in-process can carry out nimble operation, drops the plant thin slice sample concrete position on the glass piece according to the top, carries out the conditioning and scribbles the liquid and handle.
Wherein, the contact department of arm-tie 4 and screw thread seat 8 has seted up circular through-hole, and arm-tie 4 cooperation is on the draw runner 3 in drawing groove 2, and arm-tie 4 moves the back left on draw runner 3, and the cutting section of thick bamboo 6 that is located the top side of arm-tie 4 is in same axis with the sampling box 9 of drawing groove 2 bottom side for can directly stretch into in the sampling box 9 by the cutting section of thick bamboo 6 top complex ripple pull head 7 male plant stem, be convenient for realize the circular cutting sampling operation of relatively closed sample.
Wherein, the through-hole of circular structure is also seted up to the bottom surface of taking out groove 2, is equipped with the screw thread in this through-hole, and the round structure of taking out groove 2 is gone up through screw-thread fit to the sampling box 9 of round tube structure for sampling box 9 after finishing the sample can rotate takes off, accomplishes follow-up microscopical observation operation, and the rotatory circular cutting scope of cutter 14 is located the top side position of sampling box 9, utilizes cutter 14 to carry out circular cutting sampling operation to the plant stem bottommost that stretches into.
The corrugated slider 7 is of a laminated corrugated pipe structure, the bottom end of the corrugated slider is in threaded fit connection with the top end of the cutting cylinder 6, as shown in fig. 2, the corrugated slider 7 can be taken down from the top side of the cutting cylinder 6, and the corrugated slider 7 attached to different sampled plant stem structures is replaced, so that certain market expansibility is achieved.
Wherein, draw groove 2 also set up the hole structure of penetrating that is used for penetrating second connecting pipe 15, and penetrate the hole structure and be clearance fit with second connecting pipe 15, and second connecting pipe 15 passes and also has the arm-tie 4 of clearance fit hole, and be through-connected mutually with funnel 5, pour into a certain amount of culture solution liquid into by funnel 5, pour into the pipeline into, realize that liquid is downflow, and simultaneously, these clearance fit make the pipeline have nimble mobility, can not influence the normal removal of arm-tie 4.
Wherein, the partial section and the middle pipeline of overflow pipe 12 stretching into in sampling box 9 adopt the backward flow mouth design, prevent that a large amount of liquid from flowing out by the mouth of pipe, and the structure is more reasonable.
The specific use mode and function of the embodiment are as follows:
in the invention, a corrugated pull head 7 with a proper top opening is selected to be arranged on a cutting cylinder 6, a pull plate 4 is pushed to the left side along a slide bar 3, the cutting cylinder 6 and a sampling box 9 on a drawing groove 2 are positioned on the same central axis, a sampling plant stem is inserted into the sampling box 9 at the bottom side from the top opening of the corrugated pull head 7, a cutter 14 is pulled by the outside, the cutter 14 cuts a small section of plant stem ring at the top side of the sampling box 9 and naturally falls onto a sampling glass sheet placed in the sampling box 9, at the moment, a pressure air bag pump 13 is manually squeezed, culture liquid in a right side culture liquid storage tank 10 falls onto the sampling glass sheet placed in the sampling box 9, sampling liquid dropping operation is completed on the plant stem, the sampling box 9 is taken down from a round threaded hole on the drawing groove 2 after sampling is completed, a series of slice observation is realized by utilizing a pressing sheet to compact under an external microscope, whole process for plant thin slice after the section is in the device, prevents that the section of section tuber is infected to outside dust, and the culture solution that drips is also accomplished in this device, and the time of contacting with the outside air when having significantly reduced plant stem section sample, consequently to the organizational structure that follow-up microscope detected, the accuracy is higher.
It should be noted that the pressure air bag pump is the flexible pump body structure made by the gasbag, and it is used comparatively extensively, and in this structure, it was installed between the overflow pipe, can inhale its inner chamber with the liquid in the culture solution holding vessel of right side, carries out the hand-held extrusion once more, can make again that some liquid that its inner chamber was inhaled drip into the glass piece of placing in the sample box left, accomplishes the operation of dripping into of culture solution.
Claims (5)
1. The tissue slicing device for plant quarantine comprises a supporting vertical plate (1), a drawing groove (2), a slide bar (3), a pulling plate (4), a funnel (5), a cutting cylinder (6), a corrugated pull head (7), a threaded seat (8), a sampling box (9), a culture solution storage tank (10), a first connecting pipe (11), an overflow pipe (12), a pressure air bag pump (13), a cutter (14) and a second connecting pipe (15);
the method is characterized in that: the drawing groove (2) is of a cavity groove structure, supporting vertical plates (1) with plate structures are arranged at the front and the rear parts of the bottom surface of the drawing groove (2), and a sampling box (9) and a culture solution storage tank (10) are further arranged at the bottom side of the drawing groove (2) from left to right; a notch with a rectangular structure is formed in the position, close to the left side, of the rear wall of the inner cavity of the drawing groove (2), and a cutter (14) is installed in the notch in a matched mode through a support shaft; the sampling box (9) is communicated with the culture solution storage tank (10) through an overflow pipe (12), and a pressure air bag pump (13) is connected to the middle position of the overflow pipe (12); the right end of the pulling plate (4) is provided with a T-shaped block structure for pulling, the left side of the top surface of the pulling plate (4) is provided with a threaded seat (8) with a circular ring structure, the threaded seat (8) is internally connected with a cutting cylinder (6) with a cylindrical cylinder structure through threads, and the top end of the cutting cylinder (6) is also matched with a corrugated pull head (7) in a corrugated plastic pipe mode in a penetrating manner; a funnel (5) is further arranged at the right side of the top surface of the pulling plate (4); the first connecting pipe (11) and the second connecting pipe (15) are two connecting hoses connected on one pipeline, the first connecting pipe (11) is connected between the culture solution storage tank (10) and the second connecting pipe (15), and the other end of the second connecting pipe (15) is connected to the funnel (5);
a circular through hole is formed in the contact position of the pull plate (4) and the threaded seat (8), the pull plate (4) is matched with the slide bar (3) in the drawing groove (2), and after the pull plate (4) moves leftwards on the slide bar (3), the cutting cylinder (6) positioned on the top side of the pull plate (4) and the sampling box (9) at the bottom side of the drawing groove (2) are positioned on the same central axis;
the bottom surface of the drawing groove (2) is also provided with a through hole of a circular structure, a thread is arranged in the through hole, the sampling box (9) of a circular cylinder structure is matched on the circular structure on the bottom surface of the drawing groove (2) through the thread, and the rotary circular cutting range of the cutter (14) is positioned at the top side of the sampling box (9).
2. The tissue slice apparatus for plant quarantine according to claim 1, wherein: the funnel (5) is communicated with the culture solution storage tank (10) through a second connecting pipe (15) penetrating through the rear side supporting vertical plate (1), and the pipeline routing track of the second connecting pipe (15) is located at the bottom side of the pulling plate (4).
3. The tissue slice apparatus for plant quarantine according to claim 1, wherein: the junction of overflow pipe (12) and culture solution storage jar (10) is fixed, and the connected mode of overflow pipe (12) other end and sample box (9) is activity through connection, and the slim tube of scalable activity is run through in sample box (9) for the overflow pipe (12) other end promptly.
4. The tissue slice apparatus for plant quarantine according to claim 1, wherein: the corrugated pull head (7) is of a laminated corrugated pipe structure, and the bottom end of the corrugated pull head is in threaded fit connection with the top end of the cutting cylinder (6).
5. The tissue slice apparatus for plant quarantine according to claim 1, wherein: the drawing groove (2) is also provided with a penetrating hole structure used for penetrating the second connecting pipe (15), the penetrating hole structure is in clearance fit with the second connecting pipe (15), and the second connecting pipe (15) penetrates through the drawing plate (4) with the same clearance fit hole and is communicated with the funnel (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810275371.9A CN108680380B (en) | 2018-03-30 | 2018-03-30 | Tissue slice device for plant quarantine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810275371.9A CN108680380B (en) | 2018-03-30 | 2018-03-30 | Tissue slice device for plant quarantine |
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| CN108680380A CN108680380A (en) | 2018-10-19 |
| CN108680380B true CN108680380B (en) | 2020-12-11 |
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| CN201810275371.9A Active CN108680380B (en) | 2018-03-30 | 2018-03-30 | Tissue slice device for plant quarantine |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113687029B (en) * | 2021-08-20 | 2024-03-01 | 烟台海关技术中心 | Quarantine plant disease specimen collection device and application method thereof |
| CN117140646B (en) * | 2023-10-30 | 2024-01-26 | 中华人民共和国连云港海关 | Plant quarantine equipment for reducing interference of air to sample |
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| CN1309765A (en) * | 1998-05-29 | 2001-08-22 | 迈克罗姆国际股份有限公司 | Knife-holder for microtome |
| DE10048643A1 (en) * | 1999-09-22 | 2001-05-31 | Biopsytec Gmbh | Automatic sampling unit for filling microtitration plates with plant matter, comprises a stripper designed for virtually friction-free motion along a cutting stamp axis |
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| CN108680380A (en) | 2018-10-19 |
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