CN218372279U

CN218372279U - Cell culture solution sampling equipment for research and development

Info

Publication number: CN218372279U
Application number: CN202222351468.8U
Authority: CN
Inventors: 郭怀攀
Original assignee: Hefei Linmei Biotechnology Co ltd
Current assignee: Hefei Linmei Biotechnology Co ltd
Priority date: 2022-09-05
Filing date: 2022-09-05
Publication date: 2023-01-24
Anticipated expiration: 2032-09-05

Abstract

The utility model discloses a cell culture liquid sampling equipment is used in research and development relates to research and development experimental apparatus technical field, need use both hands simultaneously to current culture solution sampling equipment, inconvenient use in the actual operation process, and the culture solution can contact the spring, leads to the inside material of culture solution to receive the problem that the spring influences, now proposes following scheme, and it includes sampling tube and handle, handle fixed mounting is on the sampling tube top, the inside top fixed mounting of sampling tube has fixed pipe, fixed intraduct wall slidable mounting has the internal thread pipe, the inside top of sampling tube is rotated and is installed the threaded rod, the threaded rod is located internal thread pipe inner chamber, internal thread pipe and the meshing of internal thread pipe inner wall. The utility model discloses can take out the culture solution by the handheld handle operation sampling tube of one hand, convenient actual operation, and when the culture solution got into the check valve, the culture solution can not contact the spring, avoids the inside material of culture solution to receive the spring influence, causes the culture solution inefficacy.

Description

Cell culture solution sampling equipment for research and development

Technical Field

The utility model relates to a research and development experimental apparatus technical field especially relates to a cell culture liquid sampling equipment is used in research and development.

Background

The culture solution is also called: plant culture solution is commonly used for supplying nutrition to water culture plants. Culture broth is an artificially prepared nutrient for the growth and maintenance of microorganisms, plant and animal tissues, and generally contains carbohydrates, nitrogen-containing substances, inorganic salts, vitamins, water, and the like.

Chinese patent, application No. CN202021813903.9, patent name "a cell culture solution aseptic sampling device" proposes that a piston is driven to move up and down by rotating a screw rod, negative pressure is formed in a buffer tank, the culture solution is sucked into the buffer tank, the culture solution in the buffer tank is squeezed into a hose by pressure, and is discharged into a vessel to be transferred from a drain pipe, when the buffer tank sucks the culture solution, a second blocking mechanism can prevent external air from being sucked, and when the buffer tank discharges the culture solution, a first blocking mechanism can prevent the culture solution from flowing back into the culture vessel. Although it has been solved the culture solution and has been taken a sample through simple apparatus such as burette, the in-process of taking a sample receives the pollution of external bacterium easily, user of service in above-mentioned technical scheme needs one to hold the buffer memory jar, rotatory screw rod on the other hand, lead to unable hand of taking out to stabilize the culture dish, can produce when the sample and rock, inconvenient use in the actual operation in-process, and when the culture solution got into first separation mechanism and second separation mechanism, the culture solution can contact the spring, the spring is metallics usually, lead to the inside material of culture solution to receive the spring influence, cause the culture solution inefficacy. Therefore, in order to solve such problems, we have proposed a cell culture solution sampling device for development.

SUMMERY OF THE UTILITY MODEL

The utility model provides a pair of cell culture liquid sampling equipment is used in research and development has solved current culture liquid sampling equipment and need use both hands simultaneously, inconvenient use in the in-service operation process, and the culture solution can contact the spring, leads to the problem that the inside material of culture solution can receive the spring influence.

In order to realize the purpose, the utility model adopts the following technical scheme:

the utility model provides a research and development is with cell culture liquid sampling equipment, includes sampling tube and handle, handle fixed mounting is on the sampling tube top, the inside top fixed mounting of sampling tube has fixed pipe, fixed intraductal wall slidable mounting has the internal thread pipe, the inside top of sampling tube is rotated and is installed the threaded rod, the threaded rod is located internal thread intraductal chamber, internal thread pipe and the meshing of internal thread intraductal wall, the inside fixed mounting of handle has servo motor, servo motor output and threaded rod top fixed connection, the bottom fixed mounting of internal thread pipe has the piston, piston and sampling tube inner wall phase-match, sampling tube bottom fixed mounting has first check valve, first check valve bottom fixed mounting has the sample needle, sampling tube lateral wall bottom fixed mounting has the second check valve.

Preferably, first check valve is the same with the structure of second check valve, first check valve bottom end fixed mounting has the connecting pipe, the inside fixed mounting of first check valve has a valve section of thick bamboo, valve section of thick bamboo bottom slidable mounting has the valve plug, valve plug and connecting pipe phase-match, be connected with the spring between valve plug top and the inside top of a valve section of thick bamboo.

Preferably, the valve plug bottom is the toper structure, sample needle fixed mounting is in the connecting pipe bottom of first check valve, the connecting pipe and the sampling tube lateral wall fixed connection of second check valve.

Preferably, the circumferential side wall of the valve cylinder is fixedly provided with support rods distributed in an annular array, and the end parts of the support rods are fixedly connected with the inner wall of the first check valve.

Preferably, handle lateral wall fixed mounting has the button, the inside circumference lateral wall fixed mounting of sampling tube has the baffle, fixed cover of baffle is located fixed pipe circumference lateral wall bottom, the lateral wall of sampling tube is equipped with the observation window, the observation window lateral wall is carved with the scale.

Preferably, the internal thread pipe lateral wall fixed mounting has the gag lever post that is the symmetric distribution, the spacing groove that is the symmetric distribution is seted up to the inner wall of fixed pipe, gag lever post slidable mounting is in the spacing inslot.

The utility model has the advantages that:

1. the button is pressed to start the servo motor to drive the threaded rod to rotate, the threaded rod drives the internal thread pipe to move upwards along the limiting groove, the internal thread pipe drives the piston to move upwards along the sampling pipe, negative pressure is formed in the sampling pipe, pressure suction culture solution enters the sampling pipe from the first check valve, the button is pressed to start the servo motor to drive the threaded rod to rotate reversely, the threaded rod drives the piston to move downwards along the limiting groove, and the piston pushes the culture solution which is extruded into the second check valve to pass through the second check valve to reach a vessel for research and development.

2. Through setting up a valve section of thick bamboo and valve plug, when the culture solution inhales and discharges, pressure can promote inside the valve plug gets into a valve section of thick bamboo, and the culture solution gets into first check valve and second check valve in the back, can flow at a valve section of thick bamboo outer wall for the culture solution can not contact the spring.

In conclusion, the culture solution can be taken out by operating the sampling tube by using the device through a single hand-held handle, the actual operation is convenient, and when the culture solution enters the check valve, the culture solution cannot contact with the spring, so that the phenomenon that substances in the culture solution are influenced by the spring to cause the failure of the culture solution is avoided.

Drawings

FIG. 1 is a schematic view of the present invention;

fig. 2 is a cross-sectional view of the present invention;

fig. 3 is a sectional view of the first check valve of the present invention.

Reference numbers in the figures: 1. a sampling tube; 2. a handle; 3. a fixed tube; 4. an internally threaded tube; 5. a threaded rod; 6. a servo motor; 7. a piston; 8. a first check valve; 9. a connecting pipe; 10. a sampling needle; 11. a second check valve; 12. a limiting rod; 13. a limiting groove; 14. an observation window; 15. a graduated scale; 16. a button; 17. a valve cylinder; 18. a valve plug; 19. a support bar; 20. a spring; 21. a separator.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-2, a cell culture solution sampling device for research and development, including sampling tube 1 and handle 2, handle 2 fixed mounting is on sampling tube 1 top, the lateral wall of sampling tube 1 is equipped with observation window 14, scale 15 is carved with to observation window 14 lateral wall, 1 inside top fixed mounting of sampling tube has

fixed pipe

3, 1 inside circumference lateral wall fixed mounting of sampling tube has

baffle

21, 3 circumference lateral wall bottoms of fixed pipe are located to the fixed cover of

baffle

21, 3 inner wall slidable mounting of fixed pipe have

internal thread pipe

4, 4 lateral walls of internal thread pipe fixed mounting have gag lever post 12 that are the symmetric distribution, spacing groove 13 that is the symmetric distribution is seted up to the inner wall of fixed pipe 3, gag lever post 12 slidable mounting is in gag lever post 13.

Sampling tube 1 inside top is rotated and is installed threaded rod 5, threaded rod 5 is located 4 inner chambers of internal thread pipe, 4 inner wall meshes with internal thread pipe 4 of internal thread pipe, 2 inside fixed mounting of handle have

servo motor

6, 2 lateral wall fixed mounting of handle have

button

16, 2 inside fixed mounting of handle have the battery, 6 output of servo motor and 5 top fixed connection of threaded rod, the bottom fixed mounting of internal thread pipe 4 has piston 7,

piston

7 and 1 inner wall phase-match of sampling tube, 1 bottom fixed mounting of sampling tube has

first check valve

8, 8 bottom fixed mounting of first check valve has

sample needle

10, 1 lateral wall bottom fixed mounting of sampling tube has second check valve 11.

Referring to fig. 3, first check valve 8 is the same with structure of

second check valve

11, 8 bottom fixed mounting of first check valve have connecting

pipe

9, 8 inside fixed mounting of first check valve have valve section of

thick bamboo

17, 17 circumference lateral wall fixed mounting of valve section of thick bamboo has bracing piece 19 that is annular array and distributes, the tip and the 8 inner wall fixed connection of first check valve of bracing piece 19, valve section of thick bamboo 17 bottom slidable mounting has valve plug 18, valve plug 18 and connecting pipe 9 phase-match, be connected with spring 20 between 18 tops of valve plug and the inside top of valve section of

thick bamboo

17, 18 bottoms of valve plug are the toper structure, sampling needle 10 fixed mounting is in 9 bottoms of connecting pipe of first check valve 8, connecting pipe 9 and sampling tube 1 lateral wall fixed connection of second check valve 11.

When the device is used, a sampling needle 10 is pricked into a culture dish, a button 16 is pressed to start a servo motor 6 to drive a threaded rod 5 to rotate, the threaded rod 5 drives an internal threaded pipe 4 to move upwards along a limiting groove 13, the internal threaded pipe 4 drives a piston 7 to move upwards along a sampling pipe 1, negative pressure is formed in the sampling pipe 1, pressure pushes a valve plug 18 in a first check valve 8 to enter a valve cylinder 17, culture fluid passes through the first check valve 8 from a connecting pipe 9 in the first check valve 8 to enter the sampling pipe 1, a graduated scale 15 of an observation window 14 is compared to take out quantitative culture fluid, a spring 20 of the first check valve 8 pushes the valve plug 18 to move downwards to block the connecting pipe 9, then the button 16 is pressed to start the servo motor 6 to drive the threaded rod 5 to rotate reversely, the threaded rod 5 drives the piston 7 to move downwards along the limiting groove 13, the piston 7 pushes the culture fluid to extrude into the connecting pipe 9 of a second check valve 11, the culture fluid pushes the sampling valve plug 18 of the second check valve 11 to enter the valve cylinder 17, the culture fluid passes through the second check valve 11 to reach the culture dish which is subjected to be subjected to one-hand operation, and development, and the single-hand operation of the single-hand operation dish 1 can be realized.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a research and development is with cell culture liquid sampling device, includes sampling tube (1) and handle (2), its characterized in that, handle (2) fixed mounting is on sampling tube (1) top, sampling tube (1) inside top fixed mounting has fixed tube (3), fixed tube (3) inner wall slidable mounting has internal thread pipe (4), sampling tube (1) inside top is rotated and is installed threaded rod (5), threaded rod (5) are located internal thread pipe (4) inner chamber, internal thread pipe (4) and internal thread pipe (4) inner wall meshing, handle (2) inside fixed mounting has servo motor (6), servo motor (6) output and threaded rod (5) top fixed connection, the bottom fixed mounting of internal thread pipe (4) has piston (7), piston (7) and sampling tube (1) inner wall phase-match, sampling tube (1) bottom fixed mounting has first check valve (8), first sampling valve (8) bottom fixed mounting has sampling needle (10), lateral wall (1) bottom fixed mounting has second check valve (11).

2. A cell culture liquid sampling device for research and development according to claim 1, characterized in that, first check valve (8) is the same with the structure of second check valve (11), first check valve (8) bottom fixed mounting has connecting pipe (9), the inside fixed mounting of first check valve (8) has valve section of thick bamboo (17), valve section of thick bamboo (17) bottom slidable mounting has valve plug (18), valve plug (18) and connecting pipe (9) phase-match, be connected with spring (20) between valve plug (18) top and the inside top of valve section of thick bamboo (17).

3. The cell culture solution sampling device for research and development as claimed in claim 2, wherein the bottom end of the valve plug (18) is a conical structure, the sampling needle (10) is fixedly installed at the bottom end of the connecting pipe (9) of the first check valve (8), and the connecting pipe (9) of the second check valve (11) is fixedly connected with the side wall of the sampling pipe (1).

4. The cell culture solution sampling device for research and development according to claim 2, wherein the circumferential side wall of the valve cylinder (17) is fixedly provided with support rods (19) distributed in an annular array, and the end parts of the support rods (19) are fixedly connected with the inner wall of the first check valve (8).

5. The cell culture solution sampling device for research and development according to claim 1, wherein the button (16) is fixedly installed on the side wall of the handle (2), the partition plate (21) is fixedly installed on the side wall of the inner circumference of the sampling tube (1), the bottom end of the side wall of the circumference of the fixing tube (3) is fixedly sleeved with the partition plate (21), the side wall of the sampling tube (1) is provided with the observation window (14), and the side wall of the observation window (14) is provided with the graduated scale (15).

6. The cell culture solution sampling device for research and development according to claim 1, wherein the inner threaded tube (4) is fixedly provided with symmetrically distributed limiting rods (12) on the side wall, the inner wall of the fixed tube (3) is provided with symmetrically distributed limiting grooves (13), and the limiting rods (12) are slidably mounted in the limiting grooves (13).