CN203307336U - PCR (Polymerase Chain Reaction) tube - Google Patents
PCR (Polymerase Chain Reaction) tube Download PDFInfo
- Publication number
- CN203307336U CN203307336U CN2013203119371U CN201320311937U CN203307336U CN 203307336 U CN203307336 U CN 203307336U CN 2013203119371 U CN2013203119371 U CN 2013203119371U CN 201320311937 U CN201320311937 U CN 201320311937U CN 203307336 U CN203307336 U CN 203307336U
- Authority
- CN
- China
- Prior art keywords
- pipe
- pcr
- tube
- seal cartridge
- linear
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 238000003752 polymerase chain reaction Methods 0.000 title abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 19
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 230000007704 transition Effects 0.000 claims description 4
- 239000004433 Thermoplastic polyurethane Substances 0.000 abstract description 13
- 229920002803 thermoplastic polyurethane Polymers 0.000 abstract description 13
- 239000000443 aerosol Substances 0.000 abstract description 4
- 230000008020 evaporation Effects 0.000 abstract description 4
- 238000001704 evaporation Methods 0.000 abstract description 4
- 206010011409 Cross infection Diseases 0.000 abstract description 2
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 2
- 239000004743 Polypropylene Substances 0.000 abstract 3
- -1 Polypropylene Polymers 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 229920001155 polypropylene Polymers 0.000 abstract 1
- 238000000034 method Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012372 quality testing Methods 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 230000004308 accommodation Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a device for PCR (Polymerase Chain Reaction), and in particular to a PCR tube. The PCR tube comprises at least a tube body and a tube cover matched with the tube body. The tube body is made of a PP (Polypropylene) material and comprises a tubular main body and a cone-shaped bottom connected with the main body. The tube cover is made of a PP material. A circular seal sleeve made of a TPU (Thermoplastic Polyurethane) material is arranged at the part of the tube body, which is matched with the tube cover, and the seal sleeve is fixed to the tube body. Products prepared from TPU (Thermoplastic Polyurethane) have good elasticity and wear resistance. According to the seal sleeve made of the TPU material which is additionally arranged, the tube body and the tube cover are more tightly sealed to realize zero evaporation of contained liquid so that cross infection generated when aerosol is contained in the PCR tube is avoided.
Description
Technical field
The utility model relates to a kind of PCR reaction and uses device, especially relates to a kind of PCR pipe.
Background technology
At present, in molecular biology research and medical clinic applications process, the PCR reaction technology is indispensable.The PCR reaction is polymerase chain reaction (polymerase chain reaction, PCR), is a kind of method at external rapid amplifying specific gene or DNA sequence dna, therefore be called again the amplification in vitro method of gene.At two Oligonucleolide primers of DNA segment both sides He Yuqi both sides complementation to be amplified, after several circulations of sex change, annealing and extension, DNA cloning 2 square time doubly.The PCR pipe is for PCR instrument and sequenator.
The whole PP material that adopts of traditional PCR pipe is made, and stopping property is general, and common PCR pipe quality testing standard is below 3%.When holding aerosol, easily produce crossed contamination.The disclosed fluorescent quantitative PCR test-tube heating sealing device of Chinese utility model that is ZL200820164042.9 as the patent No. comprises upper and lower pressing plate, in upper and lower pressing plate, be embedded with respectively Heating element, press table is provided with the first hole of plant test tube, top board has and corresponding the second hole, press table the first hole, top board and screw mandrel are fixed, the feed screw nut is limited in the nut limiting plate, and top board moves up and down under leading screw and nut mechanism drives.But this device has above-mentioned defect equally, therefore need to improve existing PCR reaction tubes.
The utility model content
The utility model provides a kind of good airproof performance, folding to be easy to the PCR pipe.
The technical scheme that its technical problem that solves the utility model adopts is:
A kind of PCR pipe, the pipe lid that comprises at least one body and match with this body, described body is that the PP material is made, body comprises the main body and the tapered bottom be connected with main body of tubulose, the pipe lid is made for the PP material, the matching part of body and pipe lid is provided with the seal cartridge of the annular that the TPU material makes, and seal cartridge and body are fixed.TPU is thermoplastic polyurethane elastomerics (Thermoplastic polyurethane), and the product of making can keep good elasticity and wear resistance.The seal cartridge of the TPU material increased makes the sealing between body and pipe lid tightr, has realized holding zero evaporation of solution, the cross infection produced while having avoided holding aerosol.
As preferably, the seal cartridge top is provided with edge, the bottom surface on edge with the end face of body, coordinate and the periphery on edge concordant with the periphery at body top.This structure becomes as a whole when body is fixed together with seal cartridge, and when pipe covered folding, seal cartridge was difficult for separating with body.
As preferably, the inwall on described main body top is provided with step, and the inner peripheral surface that makes seal cartridge and the inner-wall surface smooth transition of main body are agreed with in the width of this step and the bottom of seal cartridge.
As preferably, bottom and the seal cartridge combination section of described pipe lid are provided with annular protrusion outwardly, and the seal cartridge inner-wall surface is provided with the bulge loop matched with this annular protrusion.When annular protrusion enters in body, be positioned at the below of bulge loop, bulge loop plays barrier effect to annular protrusion, improves stopping property.
As preferably, the lower surface of described tapered bottom is arc surface.The bottom of arc surface is difficult for residual reaction solution.
As preferably, this PCR pipe comprise more than two be the linear body connected with each body, match be the linear pipe lid is connected, described pipe lid and body are split-type structural.Generally according to customer demand, it is eight platoons of 10 μ l, 20 μ l that PCR control is caused to capacity, also can be the platoon of other numbers.
The beneficial effects of the utility model are: with the regular-PCR plate, compare, the utility model good leak tightness, can reach zero evaporation (common quality testing standard is below 3%), prevents the aerosol crossed contamination, easily folding.
The accompanying drawing explanation
Fig. 1 is a kind of structural representation of the present utility model;
Fig. 2 is the local structure for amplifying schematic diagram of A section in Fig. 1;
In figure: 1, body, 11, main body, 12, tapered bottom, 13, step, 2, Guan Gai, 21, annular protrusion, 3, seal cartridge, 31, edge, 32, bulge loop.
Embodiment
Below by specific embodiment, and by reference to the accompanying drawings, the technical solution of the utility model is described in further detail.Should be appreciated that enforcement of the present utility model is not limited to the following examples, any pro forma accommodation and/or change that the utility model is made all will fall into the utility model protection domain.
Embodiment 1:
A kind of PCR pipe as depicted in figs. 1 and 2, comprise that eight bodys 1 that are linear connection cover 2 with the linear pipe be connected that is with each body matches, and pipe lid and body are split-type structural.Body is that the PP material is made, and body comprises the main body 11 of tubulose and the tapered bottom 12 be connected with main body, and the lower surface of tapered bottom is arc surface.Pipe lid is made for the PP material, and the matching part that body 1 and pipe cover is provided with the seal cartridge 3 of the annular that the TPU material makes, and seal cartridge and body are fixed.The top of seal cartridge 3 be provided with outwardly along 31, the bottom surface on edge with the end face of body, coordinate and the periphery on edge concordant with the periphery at body top.The inwall on main body 11 tops is provided with a step 13, and the inner peripheral surface that makes seal cartridge and the inner-wall surface smooth transition of main body are agreed with in the width of this step and the bottom of seal cartridge.Pipe covers 2 bottom and seal cartridge combination section and is provided with annular protrusion 21 outwardly, and the seal cartridge inner-wall surface is provided with the bulge loop 32 matched with this annular protrusion.
The seal cartridge 3 that the TPU material is made makes the sealing between body and pipe lid tightr, has realized zero evaporation.
A kind of PCR pipe, be comprised of a body 1 and the pipe lid 2 that matches with this body, and body is that the PP material is made, and body comprises the main body 11 of tubulose and the tapered bottom 12 be connected with main body, and the lower surface of tapered bottom is arc surface.Pipe lid is made for the PP material, and the matching part that body 1 and pipe cover is provided with the seal cartridge 3 of the annular that the TPU material makes, and seal cartridge and body are fixed.The top of seal cartridge 3 be provided with outwardly along 31, the bottom surface on edge with the end face of body, coordinate and the periphery on edge concordant with the periphery at body top.The inwall on main body 11 tops is provided with a step 13, and the inner peripheral surface that makes seal cartridge and the inner-wall surface smooth transition of main body are agreed with in the width of this step and the bottom of seal cartridge.Pipe covers 2 bottom and seal cartridge combination section and is provided with annular protrusion 21 outwardly, and the seal cartridge inner-wall surface is provided with the bulge loop 32 matched with this annular protrusion.
In the present embodiment, body 1 and pipe cover 2 and are split-type structural, certainly, also both can be linked together.
Above-described embodiment is a kind of preferably scheme of the present utility model, not the utility model is done to any pro forma restriction, under the prerequisite that does not exceed the technical scheme that claim puts down in writing, also has other variant and remodeling.
Claims (9)
1. a PCR manages, the pipe lid (2) that comprises at least one body (1) and match with this body, it is characterized in that: described body (1) is made for the PP material, body comprises the main body (11) and the tapered bottom be connected with main body (12) of tubulose, the pipe lid is made for the PP material, the matching part of body (1) and pipe lid is provided with the seal cartridge (3) of the annular that the TPU material makes, and seal cartridge and body are fixed.
2. PCR according to claim 1 pipe, it is characterized in that: the seal cartridge top is provided with edge, the bottom surface on edge with the end face of body, coordinate and the periphery on edge concordant with the periphery at body top.
3. PCR according to claim 1 and 2 pipe, it is characterized in that: the inwall on described main body top is provided with step, and the inner peripheral surface that makes seal cartridge and the inner-wall surface smooth transition of main body are agreed with in the width of this step and the bottom of seal cartridge.
4. PCR according to claim 1 and 2 manages, and it is characterized in that: bottom and the seal cartridge combination section of described pipe lid (2) are provided with annular protrusion outwardly, and the seal cartridge inner-wall surface is provided with the bulge loop matched with this annular protrusion.
5. PCR according to claim 1 and 2 manages, and it is characterized in that: the lower surface of described tapered bottom is arc surface.
6. PCR according to claim 1 and 2 manages, and it is characterized in that: it comprises more than two that being the linear body (1) connected covers (2) with the linear pipe be connected that is with each body matches, and it is split-type structural that described pipe covers with body.
7. PCR according to claim 3 manages, and it is characterized in that: it comprises more than two that being the linear body (1) connected covers (2) with the linear pipe be connected that is with each body matches, and it is split-type structural that described pipe covers with body.
8. PCR according to claim 4 manages, and it is characterized in that: it comprises more than two that being the linear body (1) connected covers (2) with the linear pipe be connected that is with each body matches, and it is split-type structural that described pipe covers with body.
9. PCR according to claim 5 manages, and it is characterized in that: it comprises more than two that being the linear body (1) connected covers (2) with the linear pipe be connected that is with each body matches, and it is split-type structural that described pipe covers with body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013203119371U CN203307336U (en) | 2013-06-03 | 2013-06-03 | PCR (Polymerase Chain Reaction) tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013203119371U CN203307336U (en) | 2013-06-03 | 2013-06-03 | PCR (Polymerase Chain Reaction) tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203307336U true CN203307336U (en) | 2013-11-27 |
Family
ID=49613558
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013203119371U Expired - Lifetime CN203307336U (en) | 2013-06-03 | 2013-06-03 | PCR (Polymerase Chain Reaction) tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203307336U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015081872A1 (en) * | 2013-12-04 | 2015-06-11 | Gene Era Biotech Co. Ltd. | Sample tube assemblies and pipette tip with improved leakage prevention |
| WO2016188430A1 (en) * | 2015-05-25 | 2016-12-01 | Coyote Bioscience Co., Ltd. | Devices and methods for sample collection cross-reference |
| US10888865B2 (en) | 2015-07-13 | 2021-01-12 | Coyote Bioscience Co., Ltd. | Device and method for sample collection |
-
2013
- 2013-06-03 CN CN2013203119371U patent/CN203307336U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015081872A1 (en) * | 2013-12-04 | 2015-06-11 | Gene Era Biotech Co. Ltd. | Sample tube assemblies and pipette tip with improved leakage prevention |
| CN106103686A (en) * | 2013-12-04 | 2016-11-09 | 杭州金源生物技术有限公司 | There is the sample cell assembly that improved leakage is taken precautions against |
| WO2016188430A1 (en) * | 2015-05-25 | 2016-12-01 | Coyote Bioscience Co., Ltd. | Devices and methods for sample collection cross-reference |
| US10888865B2 (en) | 2015-07-13 | 2021-01-12 | Coyote Bioscience Co., Ltd. | Device and method for sample collection |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: Room 102, Building 2, No. 256, Jianghong South Road, Binjiang District, Hangzhou, Zhejiang 310000 Patentee after: Hangzhou Jinyuan Biotechnology Co.,Ltd. Address before: Room 102, Building 2, No. 256, Jianghong South Road, Binjiang District, Hangzhou, Zhejiang 310052 Patentee before: GENE ERA BIOTECH CO.,LTD. Address after: 201400 Room 201, 2/F, Building 1, No. 211, Huancheng East Road, Fengxian District, Shanghai Patentee after: Shanghai Jinyuanhui Biotechnology Co.,Ltd. Address before: Room 102, Building 2, No. 256, Jianghong South Road, Binjiang District, Hangzhou, Zhejiang 310000 Patentee before: Hangzhou Jinyuan Biotechnology Co.,Ltd. |
|
| CP03 | Change of name, title or address | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131127 |
|
| CX01 | Expiry of patent term |