CN201459144U

CN201459144U - 96-hole cell culture plate without edge effect

Info

Publication number: CN201459144U
Application number: CN2009201104017U
Authority: CN
Inventors: 张�杰
Original assignee: Wuxi Nest Biotechnology Co Ltd
Current assignee: Wuxi Nest Biotechnology Co Ltd
Priority date: 2009-07-24
Filing date: 2009-07-24
Publication date: 2010-05-12
Anticipated expiration: 2019-07-24

Abstract

The utility model relates to a cell culture plate used in cell culture in biology, in particular to a 96-hole cell culture plate without edge effect, which consists of an upper cover, a culture plate and culture plate holes. The cell culture plate is characterized in that 96 culture plate holes are formed on the culture plate and distributed by 8 multiplied by 12, each hole is regularly circular or square with equal size and shape, the bottom end of each hole is closed, and a side wall is arranged at the periphery of each hole for surrounding the hole; a groove structure is formed between the outmost ring of the holes and a side wall of the culture plate; the upper cover is flat and parallel to the plane formed by the holes on the culture plate and comprises a skirt edge; and a gap is formed between the upper cover and the culture plate; and the new groove structure is increased on the outer peripheral holes of the 96-hole cell culture. The structure can reduce the problem that the thermal power between the inner holes and the outer holes is imbalanced during the cell culture, thereby eliminating the edge effect, leading the cell growth to be more uniform and reducing the systematic errors during the experiment..

Description

A kind of 96 porocyte culture plates of non-flanged effect

Affiliated technical field

The utility model relates to the Tissue Culture Plate that culturing cell uses in the biology, Tissue Culture Plate, especially a kind of 96 porocyte culture plates of non-flanged effect.

Background technology

At present, the Tissue Culture Plate that culturing cell uses in biology, in the process of cell cultures, fringing effect takes place through regular meeting, fringing effect is meant in the cell cultures of using porous plate, cell in the peripheral hole is concentrated in the outside in hole, and is fairly obvious in 96 orifice plates that this phenomenon is used always in experiment; And the quantity of marginal pore accounts for 1/3 of sum, and this just means because the influence that this error caused is quite huge.

Because big cellulous growth can be subjected to the inhibition of exposing cell on every side, so fringing effect causes the average speed of growth of marginal pore inner cell faster than other holes, thereby in experimentation the drawing-in system error; In a lot of large-scale medicine screening experiments, people even discard the experimental result in hole on every side, this just means that per three 96 well culture plates can only be taken as two boards and use.

The reason that fringing effect produces is because after culture plate at room temperature transfers to 37 ℃ of incubators, and the temperature head that produces on the culture plate influences that cell settlement causes; For confirming this conclusion, someone has done two kinds of different experiments, first experiment is directly to put into 37 ℃ of incubators behind the cell inoculation, take out after 1 hour and examine under a microscope, most of cell of discovery apertura lateralis is all moved to the outside gathering growth in hole, rather than as the average growth of internal holes; To be Tissue Culture Plate taking out after placing for some time under 37 ℃ of conditions another experimental evidence, adds cell and do not put into 37 ℃ of environment, finds that cell is opposite sedimentation trend; These 2 suffice to show that the deciding factor that produces the cell edges effect is exactly unbalance (the BETINA KERSTIN LUNDHOLT of thermokinetics between the inner/outer hole in the cell settlement process, KURT M.SCUDDER, and LEN PAGLIARO, A SimpleTechnique for Reducing Edge Effect in Cell-Based Assays.Journal of BiomolecularScreening 2003:566-570).

The method that overcomes this phenomenon at present is divide to go into cell in culture plate after, at room temperature placed 1-2 hour, allow cell not have sedimentation under the situation of temperature head, and then transfer in 37 ℃ of incubators, this method is fit to the cell that CHO etc. at room temperature can be adherent at least; The defective of this method is that the cell ambient temperature reduction for fragility can cause cellular stress to produce, thereby change the proterties of cell, especially in former generation,, cultured cells can not bear the life condition of harshness like this more, can take place therefore that cell growth state is not good, apoptosis even death.

The reason that causes 96 at present conventional porocyte culture plates to produce fringing effect is: the hole sidewall of the 96 porocyte culture plate outer rings of using directly contacts with extraneous at present, to the unusual sensitivity of temperature, thus migration that can not regulating cell when temperature fluctuation is very big; And inboard hole is because only from the bottom contact external world, the cell growth is just very even; Change if therefore can make it only experience outside temperature, will avoid the generation of fringing effect in the lateral layout structure of apertura lateralis from the bottom.

Summary of the invention

Goal of the invention of the present utility model is: the 96 porocyte culture plates that a kind of non-flanged effect is provided, this Tissue Culture Plate is by changing the structure of original 96 orifice plates, designed the bathtub construction of different in width and thickness in the outside of outermost side opening, reach the purpose of at utmost eliminating fringing effect, guaranteed the optimum regime of cell cultures simultaneously again; To achieve these goals, the utility model is achieved in that a kind of 96 porocyte culture plates of non-flanged effect, by: loam cake, culture plate and cultivation plate hole are formed; It is characterized in that: have 96 to cultivate plate holes on the culture plate, cultivate plate hole and be 8 * 12 and distribute, each hole be the positive circle that all equates of size, shape or square, the bottom sealing, the upper end is opened wide and is convenient to the nutrient solution adding, periphery have sidewall center on the hole around; Cultivate the plane of a little higher than sidewall formation of height of plate hole; The structure that between outermost one circle hole and culture plate gusset, has groove; Loam cake is smooth, and the plane parallel with the culture plate upper surface hole forms has the shirt rim, and has the slit between the culture plate, and spacing is 0.1-2mm; The hole be distributed as rectangular, the width between centers in each hole equates that distance is 0.5-2cm; Culture plate; Wherein: outermost one circle is cultivated and is existed between plate hole and the culture plate gusset at interval on the culture plate, and distance is: 12 hole long side 1-4mm, 8 hole short brink 2-6mm; Between one circle cultivation plate hole of outermost on the culture plate and culture plate gusset, there is the groove structure, this groove structure is communicated with around being, one or more groove can be designed in four limits, perhaps four grooves that the nook is connected, the height of groove is 0-15mm, and the hole is separated from the bottom with extraneous; Wherein: the width of groove is: 12 hole long side 0.5-3.5mm, and 8 hole short brink 0.5-5.5mm, the internal diameter of groove is: 12 hole long side 0-3.5mm, 8 hole short brink 0-5.5mm can be solid grooves, its length covers all holes of periphery; Groove is detachable, be one perhaps with culture plate, groove can be that the upper end is opened wide, perhaps upper end closed, at a place or many places are porose is in communication with the outside. because this Tissue Culture Plate is by changing the structure of original 96 orifice plates, design the bathtub construction of different in width and thickness in the outside of outermost side opening, therefore reached the purpose of at utmost eliminating fringing effect, guaranteed the optimum regime of cell cultures simultaneously again.

Description of drawings

96 porocyte culture plate rough schematics of a kind of non-flanged effect of accompanying drawing.

Below in conjunction with drawings and Examples the utility model is further specified.

Embodiment

With reference to the accompanying drawings the utility model is described further below: a kind of 96 porocyte culture plates, by: loam cake, culture plate and cultivation plate hole are formed and be it is characterized in that: have 96 to cultivate plate hole on the culture plate, cultivating plate hole is 8 * 12 distributions, each hole is the positive circle that size, shape all equate, the bottom sealing, the upper end is opened wide and to be convenient to nutrient solution and to add, periphery have sidewall center on the hole around; Cultivate the plane of a little higher than sidewall formation of height of plate hole, form the outstanding groove of a circle; Loam cake can entirely cover on the culture plate, guarantees the plane parallel that forms with each culture plate hole; On cover the circle that design has projection, just in time cover top of cultivating plate hole and the spacing that keeps 0.15mm; Each width between centers of cultivating plate hole equates that distance is 1cm; Culture plate adopts transparent plastics to make; Wherein: exist at interval between outermost one circle hole and the culture plate gusset on the culture plate, distance is: 12 hole long side 1mm, 8 hole short brink 2mm; Between one circle cultivation plate hole of outermost on the culture plate and culture plate gusset, there is the groove structure, the 12 hole long side 3mm that this groove structure is communicated with around being, 8 hole short brink 4mm intercept the extraneous purpose that directly contacts with the cycle hole sidewall to reach.

Claims

1. 96 porocyte culture plates of a non-flanged effect, by: loam cake, culture plate and cultivation plate hole are formed; It is characterized in that: the hole is 8 * 12 matrix distribution, and each hole is the positive circle that all equates of size, shape or square, the bottom sealing, the upper end is opened wide and is convenient to nutrient solution and adds, periphery have sidewall center on the hole around; Cultivate the plane of a little higher than sidewall formation of height of plate hole; The structure that between outermost one circle hole and culture plate gusset, has groove.

2. 96 porocyte culture plates of non-flanged effect according to claim 1, it is characterized in that: loam cake is smooth, and the plane parallel with the culture plate upper surface hole forms has the shirt rim, and has the slit between the culture plate, and spacing is 0.1-2mm.

3. 96 porocyte culture plates of non-flanged effect according to claim 1 is characterized in that: the hole be distributed as rectangular, the width between centers in each hole equates that distance is 0.5-2cm; Outermost one circle is cultivated and is existed between plate hole and the culture plate gusset at interval on the culture plate, and distance is: 12 hole long side 1-4mm, 8 hole short brink 2-6mm;

4. 96 porocyte culture plates of non-flanged effect according to claim 1, it is characterized in that: this groove is for what be communicated with all around, and perhaps respectively there is one or more groove on four limits, perhaps four grooves that the nook is connected, the height of groove is 10-15mm, and the hole is separated from the bottom with extraneous; The width of groove is: 12 hole long side 0.5-3.5mm, and 8 hole short brink 0.5-5.5mm, the internal diameter of groove is: 12 hole long side 1-3.5mm, 8 hole short brink 1-5.5mm.

5. 96 porocyte culture plates of non-flanged effect according to claim 1 is characterized in that: groove can be solid, and its length covers all holes of periphery.

6. 96 porocyte culture plates of non-flanged effect according to claim 1, it is characterized in that: groove is detachable, is one with culture plate perhaps; Groove can be that the upper end is opened wide, and perhaps upper end closed is at a place or many places are porose is in communication with the outside.