CN104450518A - Cell culture box - Google Patents
Cell culture box Download PDFInfo
- Publication number
- CN104450518A CN104450518A CN201310542471.0A CN201310542471A CN104450518A CN 104450518 A CN104450518 A CN 104450518A CN 201310542471 A CN201310542471 A CN 201310542471A CN 104450518 A CN104450518 A CN 104450518A
- Authority
- CN
- China
- Prior art keywords
- cup
- box body
- cell culture
- box
- lid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a cell culture box. The cell culture box comprises a box body and a box cover, wherein the box body is upwardly opened, at least one cup body is fixedly connected with the bottom plane inside the box body and is respectively provided with a cup mouth which is located at the upper end of the cup body, the cup mouth of any cup body is higher than the plane at which the opening of the box body is located, a cover wall is arranged at the periphery of the box cover, the box cover is arranged above the box body and covers the cup mouth, a gap is reserved between each cup mouth and the inner side of the box cover and the lower end of the cover wall is upwards and downwards adjacent to the upper end of the wall of the box body. According to the cell culture box, the height of the cup body is increased, the cellular oxygen-supply area is increased, the position of the cup mouth is higher than that of the box body and thus the influence of pollutants inside the box body on a nutrient liquid inside the cup body is avoided, after a nutrient liquid overflows during the uniform distribution of the nutrient liquid by shaking the cup body, the nutrient liquid cannot backwards flow to the interior of the cup body and the purpose of pollution prevention is effectively achieved. By the cell culture box, good cell culture environment can be maintained and the survival rate of the cell is greatly increased.
Description
Technical field:
The present invention relates to field of human life, particularly relate to cell cultures utensil, particularly a kind of cell culture case.
Background technology:
In prior art, the outer wall of the culture hole in Tissue Culture Plate and whole culture plate is positioned in a plane, and cultured cells easily infects, oxygen undersupply.The cell survival rate cultivated is not high, does not reach use standard.
Summary of the invention:
The object of the present invention is to provide a kind of cell culture case, described this cell culture case will solve that Tissue Culture Plate cultured cells in prior art easily infects, oxygen undersupply, cell survival rate are not high, do not reach the technical problem of use standard.
This cell culture case of the present invention, comprise a box body and a lid, described box body upward opening, at least one cup is fixedly connected with in the base plane of tray interior, described cup has a rim of a cup separately, described rim of a cup is positioned at the upper end of cup, it is characterized in that: the rim of a cup of any one cup is all higher than the plane of the opening of described box body, the periphery of described lid is provided with tegmental wall, lid is arranged on the top of box body and covers rim of a cup, leave gap inside rim of a cup and lid, the lower end of tegmental wall and the upper end of box body box wall neighbouring.
Further, be fixedly connected with the cup of two or more number in the base plane of tray interior, described cup is that matrix is spaced, and is provided with gap all separately between two cups of arbitrary neighborhood.
Further, 6 ~ 96 cups are fixedly connected with in the base plane of tray interior.
Further, be provided with the stiffening plate of two or more number between the base plane of tray interior and box body box wall, described stiffening plate arranges along the peripheral intervals of the base plane of tray interior.
Further, the rim of a cup of any one cup is all higher than box opening plane 2.5-6 millimeter.
Further, the lower periphery outside box body is provided with anti-skidding burr.
Further, box body and lid are made by polystyrene material.
Further, to being arranged with circular bulge loop in lid bottom surface, the number of described circular bulge loop is equal with the number of cup, and any one circular bulge loop is arranged on the top of a cup and coaxial with cup all separately.
Further, described box body and the joint of lid are symmetrically arranged with at least two prisms, make to there is gap between lid and rim of a cup.
The present invention compares with prior art, and its effect is actively with obvious.Invention increases the height that cup is suitable for reading, increase cell oxygen supply area, rim of a cup, higher than box body, avoids the impact that nutritive medium in cup is subject to box body internal contamination thing, after rocking cup and making nutritive medium equally distributed process Middle nutrition hydrorrhea go out not in reflux cup, effectively reach anti-pollution object.The present invention can keep a good cell culture environment; Substantially increase the survival rate of cell.
Accompanying drawing explanation
Fig. 1 is the exploded side structural representation of cell culture case of the present invention.
Fig. 2 is the plan structure schematic diagram of cell culture case of the present invention
Embodiment:
Embodiment 1:
As depicted in figs. 1 and 2, cell culture case of the present invention, comprise a box body 1 and a lid 2, described box body 1 upward opening, at least one cup 3 is fixedly connected with in the base plane of box body 1 inside, described cup 3 has a rim of a cup 4 separately, described rim of a cup 4 is positioned at the upper end of cup 3, wherein, the rim of a cup 4 of any one cup 3 is all higher than the plane of the opening of described box body 1, the periphery of described lid 2 is provided with tegmental wall, gap is left inside rim of a cup 4 and lid 2, lid 2 is arranged on the top of box body 1 and covers rim of a cup 4, the lower end of tegmental wall and the upper end of box body 1 box wall neighbouring.
Further, be fixedly connected with the cup 3 of two or more number in the base plane of box body 1 inside, described cup 3 is spaced in matrix, is provided with gap 5 all separately between two cups 3 of arbitrary neighborhood.
Further, 6 ~ 96 cups 3 are fixedly connected with in the base plane of box body 1 inside.
Further, be provided with the stiffening plate 6 of two or more number between the base plane of box body 1 inside and box body 1 box wall, described stiffening plate 6 arranges along the peripheral intervals of the base plane of box body 1 inside.
Further, the rim of a cup 4 of any one cup 3 is all higher than box body 1 plane of the opening 2.5-6 millimeter.
Further, the lower periphery outside box body 1 is provided with anti-skidding burr 7.
Further, box body 1 and lid 2 are made by polystyrene material.
Further, to being arranged with circular bulge loop (not shown) in lid bottom surface, the number of described circular bulge loop is equal with the number of cup, and any one circular bulge loop is arranged on the top of a cup and coaxial with cup all separately.Circular bulge loop can prevent the pollution between two adjacent rims of a cup 4.
Further, described box body 1 and the joint of lid 2 are symmetrically arranged with at least two prisms 8, make to there is gap between lid 2 and rim of a cup 4.
Embodiment adds the height of cup 3, increase cell oxygen supply area, rim of a cup 4 is higher than box body 1, avoid the impact that nutritive medium in cup 3 is subject to box body 1 internal contamination thing, after rocking cup 3 and making nutritive medium equally distributed process Middle nutrition hydrorrhea go out not in reflux cup, effectively reach anti-pollution object.The present invention can keep a good cell culture environment; Substantially increase the survival rate of cell.
Claims (9)
1. a cell culture case, comprise a box body and a lid, described box body upward opening, at least one cup is fixedly connected with in the base plane of tray interior, described cup has a rim of a cup separately, described rim of a cup is positioned at the upper end of cup, it is characterized in that: the rim of a cup of any one cup is all higher than the plane of the opening of described box body, the periphery of described lid is provided with tegmental wall, lid is arranged on the top of box body and covers rim of a cup, leave gap inside rim of a cup and lid, the lower end of tegmental wall and the upper end of box body box wall neighbouring.
2. cell culture case as claimed in claim 1, it is characterized in that: the cup being fixedly connected with two or more number in the base plane of tray interior, described cup is that matrix is spaced, and is provided with gap all separately between two cups of arbitrary neighborhood.
3. cell culture case as claimed in claim 2, is characterized in that: be fixedly connected with 6 ~ 96 cups in the base plane of tray interior.
4. cell culture case as claimed in claim 1, it is characterized in that: the stiffening plate being provided with two or more number between the base plane of tray interior and box body box wall, described stiffening plate arranges along the peripheral intervals of the base plane of tray interior.
5. cell culture case as claimed in claim 1, is characterized in that: the rim of a cup of any one cup is all higher than box opening plane 2.5-6 millimeter.
6. cell culture case as claimed in claim 1, is characterized in that: the lower periphery outside box body is provided with anti-skidding burr.
7. cell culture case as claimed in claim 1, is characterized in that: box body and lid are made by polystyrene material.
8. cell culture case as claimed in claim 1, it is characterized in that: to being arranged with circular bulge loop in lid bottom surface, the number of described circular bulge loop is equal with the number of cup, and any one circular bulge loop is arranged on the top of a cup and coaxial with cup all separately.
9. cell culture case as claimed in claim 1, is characterized in that: described box body and the joint of lid are symmetrically arranged with at least two prisms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310542471.0A CN104450518A (en) | 2013-11-05 | 2013-11-05 | Cell culture box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310542471.0A CN104450518A (en) | 2013-11-05 | 2013-11-05 | Cell culture box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104450518A true CN104450518A (en) | 2015-03-25 |
Family
ID=52897277
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310542471.0A Pending CN104450518A (en) | 2013-11-05 | 2013-11-05 | Cell culture box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104450518A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201459144U (en) * | 2009-07-24 | 2010-05-12 | 无锡耐思生物科技有限公司 | 96-hole cell culture plate without edge effect |
| CN201598291U (en) * | 2010-01-15 | 2010-10-06 | 桂林医学院 | Combined type cell culture plate |
| CN102943039A (en) * | 2012-10-30 | 2013-02-27 | 无锡耐思生物科技有限公司 | Cell culture plate structure |
-
2013
- 2013-11-05 CN CN201310542471.0A patent/CN104450518A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201459144U (en) * | 2009-07-24 | 2010-05-12 | 无锡耐思生物科技有限公司 | 96-hole cell culture plate without edge effect |
| CN201598291U (en) * | 2010-01-15 | 2010-10-06 | 桂林医学院 | Combined type cell culture plate |
| CN102943039A (en) * | 2012-10-30 | 2013-02-27 | 无锡耐思生物科技有限公司 | Cell culture plate structure |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150325 |