CN202658162U - Multi-chamber co-culture device - Google Patents
Multi-chamber co-culture device Download PDFInfo
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- CN202658162U CN202658162U CN 201220307408 CN201220307408U CN202658162U CN 202658162 U CN202658162 U CN 202658162U CN 201220307408 CN201220307408 CN 201220307408 CN 201220307408 U CN201220307408 U CN 201220307408U CN 202658162 U CN202658162 U CN 202658162U
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- room
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- penetrating
- multicell
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- 238000003501 co-culture Methods 0.000 title claims abstract description 14
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 5
- 230000000149 penetrating Effects 0.000 claims description 41
- 239000011148 porous material Substances 0.000 claims description 3
- 244000005700 microbiome Species 0.000 abstract description 18
- 230000003993 interaction Effects 0.000 description 12
- 239000000126 substance Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000005755 formation reaction Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 230000000813 microbial Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000001070 adhesive Effects 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000035605 chemotaxis Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000002808 molecular sieve Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 231100000776 Exotoxin Toxicity 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229920002521 Macromolecule Polymers 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 206010063834 Oversensing Diseases 0.000 description 1
- 230000005167 amoeboid movement Effects 0.000 description 1
- 230000001580 bacterial Effects 0.000 description 1
- 230000034196 cell chemotaxis Effects 0.000 description 1
- 230000000295 complement Effects 0.000 description 1
- 230000030944 contact inhibition Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 239000002095 exotoxin Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000009114 investigational therapy Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000002906 microbiologic Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000000607 poisoning Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000004083 survival Effects 0.000 description 1
Abstract
The utility model relates to a multi-chamber co-culture device. A culture hole, a culture dish or a culture bottle is horizontally divided into multiple chambers (two chambers, three chambers or four chambers) through permeable membranes. The permeable membranes can be made of any material and has certain rigidity, and the membrane aperture of the permeable membranes is less than 50 mu m. The multi-chamber co-culture device is suitable for non-contact co-culture of a variety of cells or micro-organisms.
Description
Technical field
The present invention relates to a kind of multicell co-culture device, with penetrating film one culture hole, culture dish or culturing bottle level are divided into multicell, the permeability of restrictive chemicals or some particle is applicable to the co-cultivation of various kinds of cell or microorganism.
Background technology
The interaction of cell and microorganism (such as virus, bacterium, fungi) is that organic sphere generally occurs.The common cultivation of cell or microorganism is the basic means of research cell and microbial interaction.Early stage scientific research is with the research of single cell or single microorganism, and the research of cell interaction is less, and the research of cell and microbial interaction is also less.Along with the increase of the depth of investigation, these interact and in fact to belong to exchanging between information interchange between a kind of intercellular information interchange (cross-talk), the microorganism and cell and the microorganism.This information interchange all has a great impact microorganism and cell, affects their propagation, differentiation, material secretion.
This information interchange has two kinds of manifestation modes.A kind of is direct mode: namely, thereby cell directly contacts the life style that affects cell or microorganism with cell, such as contact inhibition phenomenon of poisoning intrusion cell, normal cell growth etc.Another kind then is indirect mode: namely cell or Microbiological release go out chemical substance, thereby affect the life style of other cell or microorganism by solution diffusion, such as the release of endocrine substance, and the effect of bacterial exotoxin.
Because the search time of repercussion study is longer, the research of direct interaction is difficulty comparatively.Because different cell co-cultivations, different cell growth cycles are different, and probably the grow living space of the another kind of cell of too fast contention of a kind of cell is not easy to observe; The inconvenience of what is more important mixed culture cell is distinguished, and causes cell in-situ to be identified and has certain difficulty.What is particularly worth mentioning is that cell and microorganism are difficult to cultivate altogether, because mixed culture inevitably causes necrocytosis, research can't be carried out.By contrast, the Indirect Interaction of iuntercellular or cell and microorganism then is conducive to scientific research personnel's discovery information interchange therebetween, has also found a lot of important phenomenon, and for preventing and curing diseases, the biological behaviour of control cell and microorganism provides strategy.
Yet aspect the research of cell, microorganism Indirect Interaction, traditional research means adopts Transwell to carry out.Transwell puts into a sieve cup in culture hole (holes of 24 orifice plates commonly used), different cells are placed in sieve cup or the hole cultivate the variation of observation of cell.The bottom surface of sieve cup is to be the mesh film shutoff of 10-20 μ m with the aperture, and cell can not passively pass the sieve cup and enter in the hole, but the chemical substance of emiocytosis then can freely pass through, thereby realizes the information interchange (two penetrating Room about being equivalent to) of different cells.By amoeboid movement, the mesh film that cell can be by Transwell, Transwell mainly utilize this principle can be used for the Chemotaxis ability of observation of cell.
This device of Transwell goes on the market, and is mainly produced by foreign vendor, also is widely used in the scientific research of cell chemotaxis motion aspect, but there is critical defect in the Transwell device aspect the information interchange of research cell.
1, Chemotaxis is a kind of mode of cell information interchange, because the mesh film of sieve cup is opaque, Transwell can only endpoint observation, can not realize dynamically.
2, Transwell is that a sieve glass holder is placed in another hole, and what be difficult to do is very little, so the sieve cup of Transwell generally can only be placed in 24 orifice plates or the larger orifice plate.
3, the mesh film is in the bottom of Transwell sieve cup, at the bottom of preventing that the mesh film is attached to the hole, certain distance at the bottom of the hole in the mesh film is necessary, nutrient solution in this inevitable requirement hole must have higher height nerve of a covering pore membrane, this wastes substratum on the one hand, the amount that also means on the other hand reagent (medicine) also will increase, and this is unfavorable to rare precious reagent (medicine) research.
For the deficiencies in the prior art and new technical need, a kind of multicell co-culture device of special invention is fit to research cell-cell interaction and cell and microbial interaction.
Summary of the invention
A kind of multicell co-culture device adopts penetrating film that culture hole, culture dish or culturing bottle horizontal subdivision are become multicell (two Room, three Room or four Room).Penetrating membrane material is not limit, but has certain rigidity, and the membrane pore size of penetrating film is less than 50 microns.
The present invention will have culture hole by permselective membrane or the culturing bottle level is divided into multicell, thereby be implemented in a culture hole or a culturing bottle can be cultivated various kinds of cell or microorganism, avoid the direct contact between cell, microorganism can ensure that chemicals mass-energy is freely penetrating simultaneously by penetrating film, and can observe easily the survival condition of various cells or microorganism.
Aspect putting into practice, prepare first the penetrating film with certain rigidity, with the little groove that existing culture hole file one and penetrating film are complementary, being installed on wherein penetrating film also, the bonding culture hole that is about to is divided into multicell.Similarly, penetrating film is installed in then realizes the multicell culturing bottle in the culturing bottle.Because this device is applicable to the common cultivation of cell or microorganism, this device all adopts sterilizable material to prepare in gnotobasis, perhaps carries out aseptically process after the preparation.
In practice, the penetrating degree of different chamber determines by penetrating film, selects the penetrating film in different apertures then can realize the selective permeation of chemical substance.The penetrating film of selection molecular sieve bore diameter 1k is the small molecules chemical substance of main penetrating molecular weight below 1000 then, the penetrating film of employing molecular sieve bore diameter 30k is the macromolecular substance such as the small molecules chemical substance below the main penetrating molecular weight 30k and protein then, adopt the penetrating film of aperture 0.22 μ m or 0.45 μ m then can pass through the particles such as mycoplasma, virus, the penetrating film of employing aperture 1-20 μ m then may be by particles such as bacterium and cells.
Advantage of the present invention is: 1) adopt penetrating film directly to cut apart culture hole or culturing bottle, the multicell culture apparatus both can be done very littlely (such as being easy to make two Room, 96 orifice plates) easily, also can do to get by large (such as the multicell culturing bottle), the present invention has the larger scope of application like this; 2) be perpendicular to culture hole or culturing bottle level attitude cutting apart of multicell, do not hinder like this observation of co-cultured cell, therefore be conducive to finish the dynamic observation of common cultivation; 3) the penetrating film of the different transparent performances of employing is conducive to the interaction that selectivity research small molecules, macromole, small-particle and macrobead cause like this.
Description of drawings
Fig. 1 is the device synoptic diagram that the present invention makes up penetrating two Room.
Fig. 2 is the synoptic diagram of the present invention take penetrating two Room as cell formation 96 orifice plates.
Fig. 3 is the device synoptic diagram that the present invention makes up penetrating three Room.
Fig. 4 is the synoptic diagram of the present invention take penetrating three Room as cell formation 6 orifice plates.
Fig. 5 is the device synoptic diagram that the present invention makes up two Room culturing bottles.
Among the above figure: 1. the culture hole of intending making up two Room, 2. intend making up the culture hole groove of two Room, 3. the penetrating film in two Room, the two Room culture hole that 4. build 5. intend making up the culture hole of three Room, 6. intend making up the culture hole groove of three Room, 7. the penetrating film in three Room, the three Room culture hole that 8. build, the 9. penetrating film of culturing bottle, 10. culturing bottle, the 11. two Room culturing bottles that build.
Embodiment
1, two Room culture hole cell interaction culture apparatuses.
Prepare to intend making up the culture hole (1) of two Room, file intends making up culture hole groove (2) and the coated with adhesive of two Room together in the hole, other prepares two Room with certain rigidity with penetrating film (3), snaps in two Room in the culture hole groove (2) of intending structure two Room with penetrating film (3); Namely prepare what a two Room culture hole (4) after bonding, referring to Fig. 1.The single two Room culture hole (4) that prepare suitably amplification just become two Room culture dish.
Take the two Room culture hole (4) that prepare as the unit, adjust the size of culture hole, can prepare take penetrating two Room as cell formation 96 orifice plates, referring to Fig. 2.Similarly also can make other forms of two Room porous plates.
2, three Room culture hole cell interaction culture apparatuses
Prepare to intend making up the culture hole (5) of three Room, in the hole, file out plan and make up culture hole groove (6) and the coated with adhesive of three Room, other prepares three Room with certain rigidity with penetrating film (7), snaps in three Room in the culture hole groove (6) of intending structure three Room with penetrating film (7); Namely prepare what a three Room culture hole (8) after bonding, referring to Fig. 3.The single two Room culture hole (8) that prepare suitably amplification just become three Room culture dish.
Take the two Room culture hole (8) that prepare as the unit, adjust the size of culture hole, can prepare take penetrating three Room as cell formation 24 orifice plates, referring to Fig. 4.Similarly also can make other forms of three Room porous plates.
3, two Room culturing bottles
With the penetrating film of the culturing bottle that designs (9), be adhered to culturing bottle (10) middle part, can prepare the two Room culturing bottles (11) that build, as shown in Figure 5.
More than enumerated by penetrating film preparation two Room and three Room co-culture devices, similarly, also can be by penetrating film preparation four Room or multicell co-culture device more.
Claims (4)
1. a multicell co-culture device adopts penetrating film that culture hole, culture dish or culturing bottle horizontal subdivision are become multicell.
2. multicell co-culture device according to claim 1, it is characterized in that: culture hole, culture dish or culturing bottle can be separated into two Room, three Room or four Room.
3. multicell co-culture device according to claim 1, it is characterized in that: penetrating membrane material is not limit, but has certain rigidity.
4. multicell co-culture device according to claim 1, it is characterized in that: the membrane pore size of penetrating film is less than 50 microns.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220307408 CN202658162U (en) | 2012-06-28 | 2012-06-28 | Multi-chamber co-culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220307408 CN202658162U (en) | 2012-06-28 | 2012-06-28 | Multi-chamber co-culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202658162U true CN202658162U (en) | 2013-01-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220307408 Expired - Fee Related CN202658162U (en) | 2012-06-28 | 2012-06-28 | Multi-chamber co-culture device |
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| CN (1) | CN202658162U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703318A (en) * | 2012-06-28 | 2012-10-03 | 段为钢 | Multi-chamber co-culture device |
| CN104877905A (en) * | 2015-05-04 | 2015-09-02 | 清华大学深圳研究生院 | Cell in-vitro co-culture microfluidic chip, system and method |
| CN105044176A (en) * | 2015-08-06 | 2015-11-11 | 浙江大学 | Microorganism galvanotaxis determination device and method |
| CN106103691A (en) * | 2014-04-14 | 2016-11-09 | 株式会社岛津制作所 | Cell is cultivated with equipment, cell cultivation system and cell culture processes |
| CN106170540A (en) * | 2014-05-09 | 2016-11-30 | 东洋制罐集团控股株式会社 | Multi-chamber culture vessel and cell culture processes |
| CN109337804A (en) * | 2013-07-05 | 2019-02-15 | 爱特医疗有限公司 | A kind of equipment for monitoring biomaterial development |
-
2012
- 2012-06-28 CN CN 201220307408 patent/CN202658162U/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703318A (en) * | 2012-06-28 | 2012-10-03 | 段为钢 | Multi-chamber co-culture device |
| CN109337804A (en) * | 2013-07-05 | 2019-02-15 | 爱特医疗有限公司 | A kind of equipment for monitoring biomaterial development |
| CN109337804B (en) * | 2013-07-05 | 2022-07-08 | 爱特医疗有限公司 | A equipment for monitoring biomaterial is developed |
| CN106103691A (en) * | 2014-04-14 | 2016-11-09 | 株式会社岛津制作所 | Cell is cultivated with equipment, cell cultivation system and cell culture processes |
| CN108841728A (en) * | 2014-04-14 | 2018-11-20 | 株式会社岛津制作所 | Cell culture equipment, cell culture system and cell culture processes |
| US10301586B2 (en) | 2014-04-14 | 2019-05-28 | Shimadzu Corporation | Cell culturing device, cell culturing system and cell culturing method |
| CN106170540A (en) * | 2014-05-09 | 2016-11-30 | 东洋制罐集团控股株式会社 | Multi-chamber culture vessel and cell culture processes |
| CN106170540B (en) * | 2014-05-09 | 2018-07-20 | 东洋制罐集团控股株式会社 | Multi-chamber culture vessel and cell culture processes |
| CN104877905A (en) * | 2015-05-04 | 2015-09-02 | 清华大学深圳研究生院 | Cell in-vitro co-culture microfluidic chip, system and method |
| CN105044176A (en) * | 2015-08-06 | 2015-11-11 | 浙江大学 | Microorganism galvanotaxis determination device and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130109 Termination date: 20150628 |
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| EXPY | Termination of patent right or utility model |