CN104371923A - Cell culture plate and plate cover structure thereof - Google Patents
Cell culture plate and plate cover structure thereof Download PDFInfo
- Publication number
- CN104371923A CN104371923A CN201410744640.3A CN201410744640A CN104371923A CN 104371923 A CN104371923 A CN 104371923A CN 201410744640 A CN201410744640 A CN 201410744640A CN 104371923 A CN104371923 A CN 104371923A
- Authority
- CN
- China
- Prior art keywords
- plate
- linker
- supporter
- lid
- plate lid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
Abstract
The invention provides a cell structure plate and a plate cover structure thereof. By the cell culture plate and the plate cover structure thereof, the problem of breathability of plate covers during stacking of cell culture plates and stability in connection between the plate covers and hole plates and stability in connection among the plate covers, silicon rubber mats and breathable films can be improved. The cell culture plate comprises a plate cover and a hole plate, a breathable film and a silicon rubber mat are arranged below the plate cover sequentially, supports are arranged on the plate cover, the upper end faces of the supports are planes and parallel to the upper end face of the plate cover, the lower ends of the supports are provided with connectors, the connectors penetrate the hole plate, the breathable film and the silicon rubber mat and then are inserted into gaps between adjacent culture holes of the hole plate.
Description
Technical field
The present invention relates to Tissue Culture Plate, be specifically related to the structure of Tissue Culture Plate and plate lid thereof.
Background technology
Along with molecular biological development, the heterogenous expression technology of albumen is ripe gradually and be widely used.The expression of foreign protein needs to select suitable expressive host and Expression element, and this just needs to build huge expression library, therefrom selects preferably expression vector.Tissue Culture Plate can utilize less nutrient solution to carry out the screening of high-flux cell cultivation and bacterial classification, cell strain and expression library, has fast, high-throughput, the feature such as cost-saving.
Tissue Culture Plate and shaking table with the use of time, in order to better utilised shaking table solid space, increase and cultivate flux, available multi-layer cellular culture plate fixture and Tissue Culture Plate with the use of, but also need the ventilation property of guarantee plate lid when Tissue Culture Plate stacks.And conventional " sandwich " formula plate lid sealing of existing Tissue Culture Plate, but the plate lid of sandwich style and orifice plate with the use of time, because Tissue Culture Plate is thinner, during transfer culture plate, plate lid easily slides, cause microbiological contamination risk, and the stability connected between the composition stainless steel plate lid of " sandwich ", silicagel pad, air-permeable envelope can not ensure.
Summary of the invention
For the problems referred to above, the invention provides the structure of Tissue Culture Plate and plate lid thereof, it can solve the problem of plate lid ventilation property when Tissue Culture Plate stacks, and the steadiness that can improve steadiness that plate lid and orifice plate connect and plate lid, connect between silicagel pad and air-permeable envelope.
Tissue Culture Plate, comprise plate lid and orifice plate, air-permeable envelope and silicagel pad is provided with successively below described plate lid, described plate covers and is provided with supporter, the upper surface of described supporter is plane and is parallel to the upper surface of described plate lid, the lower end of described supporter is provided with linker, and described linker is plugged in after running through described orifice plate, described air-permeable envelope and described silicagel pad in the gap between the adjacent culture hole of described orifice plate.
Further, the lower surface of described orifice plate is provided with the locating slot coordinated with described supporter, and the top of described supporter is plugged in described locating slot.
Further, the bottom of described linker is arranged with gum cover, and the upper surface of described gum cover is against the lower surface of described silicagel pad.
The structure of the plate lid of Tissue Culture Plate, the upper surface of plate lid is provided with some supporters, and the upper surface of described supporter is plane and is parallel to the upper surface of described plate lid.
Further, the bottom of described supporter is provided with linker, and described linker is fixedly connected with described plate lid.
Further, the bottom of described supporter is provided with linker, and described linker runs through air-permeable envelope, silicagel pad below described plate lid and described plate lid, and can be plugged in the gap between the adjacent culture hole of orifice plate.
Further, the bottom of described linker is arranged with gum cover, and the upper surface of described gum cover is against the lower surface of described silicagel pad.
After adopting structure of the present invention, its beneficial effect is: the upper surface of plate lid is provided with some supporters, makes to leave space between the flanking cell culture plate that stacks, is convenient to air circulation between Tissue Culture Plate; The lower surface of orifice plate is provided with the locating slot coordinated with supporter, and the top of supporter is plugged in locating slot, and can play positioning action, and preventing board lid slides; The bottom of supporter is provided with linker, linker runs through air-permeable envelope, silicagel pad below Ban Gai and plate lid, air-permeable envelope, silicagel pad clamping is with on linker, be convenient to open culture plate lid under clean environment, inject in culture plate or take out liquid, linker can be plugged in the gap between the adjacent culture hole of orifice plate, improves the steadiness that plate lid is connected with orifice plate; The bottom of linker is arranged with gum cover, and the upper surface of gum cover, against the lower surface of silicagel pad, both can prevent air-permeable envelope, silicagel pad comes off, and can guarantee that again linker is stablized in the gap that is plugged between adjacent culture hole.
Accompanying drawing explanation
Fig. 1 is the structural representation of Tissue Culture Plate of the present invention;
Fig. 2 is the plan structure schematic diagram of Fig. 1;
Fig. 3 is the structural representation of plate lid of the present invention.
Embodiment
Embodiment 1
As shown in Figure 1 and Figure 2, Tissue Culture Plate, comprise plate lid 1 and orifice plate, air-permeable envelope 2 and silicagel pad 3 is provided with successively below plate lid 1, plate lid 1 is provided with supporter 4, the upper surface 5 of supporter 4 is plane and is parallel to the upper surface of plate lid 1, the lower end of supporter 4 is provided with linker 6, linker 6 is plugged in the gap 10 between the adjacent culture hole of orifice plate 9 after running through orifice plate, air-permeable envelope 2 and silicagel pad 3, the lower surface of orifice plate is provided with the locating slot coordinated with supporter 4, and the top of supporter 4 is plugged in locating slot; The bottom of linker 6 is arranged with gum cover 7, and the upper surface of gum cover 7 is against the lower surface of silicagel pad 3.
Embodiment 2
As shown in Figure 3, the structure of the plate lid of Tissue Culture Plate, the upper surface of plate lid 1 is provided with some supporters 4, and the upper surface 5 of supporter 4 is plane and is parallel to the upper surface of plate lid 1; The bottom of supporter 4 is provided with linker 6, and linker 6 runs through air-permeable envelope 2, silicagel pad 3 below plate lid 1 and plate lid 1, and can be plugged in the gap between the adjacent culture hole of orifice plate, and 8 is the hole wall of cell culture well; The bottom of linker 6 is arranged with gum cover 7, and the upper surface of gum cover 7 is against the lower surface of silicagel pad 3.
Claims (7)
1. Tissue Culture Plate, comprise plate lid and orifice plate, air-permeable envelope and silicagel pad is provided with successively below described plate lid, described plate covers and is provided with supporter, the upper surface of described supporter is plane and is parallel to the upper surface of described plate lid, the lower end of described supporter is provided with linker, and described linker is plugged in after running through described orifice plate, described air-permeable envelope and described silicagel pad in the gap between the adjacent culture hole of described orifice plate.
2. Tissue Culture Plate according to claim 1, is characterized in that: the lower surface of described orifice plate is provided with the locating slot coordinated with described supporter, and the top of described supporter is plugged in described locating slot.
3. Tissue Culture Plate according to claim 1 and 2, is characterized in that: the bottom of described linker is arranged with gum cover, and the upper surface of described gum cover is against the lower surface of described silicagel pad.
4. the structure of the plate lid of Tissue Culture Plate, the upper surface of plate lid is provided with some supporters, and the upper surface of described supporter is plane and is parallel to the upper surface of described plate lid.
5. the structure of the plate lid of Tissue Culture Plate according to claim 4, is characterized in that: the bottom of described supporter is provided with linker, described linker is fixedly connected with described plate lid.
6. the structure of the plate lid of Tissue Culture Plate according to claim 5, it is characterized in that: the bottom of described supporter is provided with linker, described linker runs through air-permeable envelope, silicagel pad below described plate lid and described plate lid, and can be plugged in the gap between the adjacent culture hole of orifice plate.
7. the structure of the plate lid of the Tissue Culture Plate according to claim 5 or 6, is characterized in that: the bottom of described linker is arranged with gum cover, and the upper surface of described gum cover is against the lower surface of described silicagel pad.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410744640.3A CN104371923B (en) | 2014-12-09 | 2014-12-09 | Tissue Culture Plate and its plate lid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410744640.3A CN104371923B (en) | 2014-12-09 | 2014-12-09 | Tissue Culture Plate and its plate lid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104371923A true CN104371923A (en) | 2015-02-25 |
| CN104371923B CN104371923B (en) | 2017-04-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410744640.3A Active CN104371923B (en) | 2014-12-09 | 2014-12-09 | Tissue Culture Plate and its plate lid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104371923B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1477936A (en) * | 2000-10-17 | 2004-02-25 | �����塤������ŵ | Fixing and/or assembling device |
| CN202017021U (en) * | 2011-04-29 | 2011-10-26 | 弗曼燊生物科技(上海)有限公司 | Microbial culture device |
| CN103013829A (en) * | 2012-12-28 | 2013-04-03 | 江南大学 | Structure of deep-well cell culture plate cover |
| CN204434633U (en) * | 2014-12-09 | 2015-07-01 | 江南大学 | The structure of a kind of Tissue Culture Plate and plate lid thereof |
-
2014
- 2014-12-09 CN CN201410744640.3A patent/CN104371923B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1477936A (en) * | 2000-10-17 | 2004-02-25 | �����塤������ŵ | Fixing and/or assembling device |
| CN202017021U (en) * | 2011-04-29 | 2011-10-26 | 弗曼燊生物科技(上海)有限公司 | Microbial culture device |
| CN103013829A (en) * | 2012-12-28 | 2013-04-03 | 江南大学 | Structure of deep-well cell culture plate cover |
| CN204434633U (en) * | 2014-12-09 | 2015-07-01 | 江南大学 | The structure of a kind of Tissue Culture Plate and plate lid thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104371923B (en) | 2017-04-05 |
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Effective date of registration: 20190627 Address after: 210000 Building B, Zhongdan Eco-life Science Industrial Park, 3-1 Xinjinhu Road, Jiangbei New District, Nanjing City, Jiangsu Province, 2005-2010 Patentee after: Nanjing Diji Biotechnology Co., Ltd. Address before: 214000 1800 Lihu Avenue, Binhu District, Wuxi, Jiangsu Patentee before: Jiangnan University |
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| TR01 | Transfer of patent right |