CN104513794A - S-shaped photobioreactor system - Google Patents

S-shaped photobioreactor system Download PDF

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Publication number
CN104513794A
CN104513794A CN201510036773.XA CN201510036773A CN104513794A CN 104513794 A CN104513794 A CN 104513794A CN 201510036773 A CN201510036773 A CN 201510036773A CN 104513794 A CN104513794 A CN 104513794A
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China
Prior art keywords
bioreactor
snakelike
photo
tank
carbonic acid
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CN201510036773.XA
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Inventor
迈克尔·威尔逊
塞缪尔·莫尔顿三世
杰克·格洛波
马克·克罗克
安得烈·普拉西多
托马斯·刘
项羽·张
张恒
刘俊国
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LIANHENGHUI INVESTMENT Co Ltd
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LIANHENGHUI INVESTMENT Co Ltd
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Priority to CN201510036773.XA priority Critical patent/CN104513794A/en
Publication of CN104513794A publication Critical patent/CN104513794A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/06Tubular
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH

Abstract

The invention discloses an S-shaped photobioreactor system which comprises an intermediate tank, a detector, a running pump, a photobioreactor, a conveying pipeline, an oil return manifold and a harvesting tank, wherein the intermediate tank is connected with the photobioreactor via the conveying pipeline and the running pump; a product out of the photobioreactor passes to a harvesting port via the oil return manifold and enters the harvesting tank; liquid out of the harvesting tank enters the intermediate tank for nutrient return via ultraviolet sterilization; gas out of the harvesting port and added carbon dioxide enter the intermediate tank via head space cycle; and the amount entering the photobioreactor from the intermediate tank is controlled through the detector. The S-shaped photobioreactor overcomes the problems of lack of temperature and illumination, nonuniform mixing, small volume, high contamination possibility, difficult maintenance and extraction and the like which almost other photobioreactors have. The S-shaped photobioreactor realizes automatic closed culture.

Description

" snakelike " photo-bioreactor system
Technical field
The present invention relates to a kind of photo-bioreactor system, relate to especially and a kind of cultivate multiple photosynthetic microorganism by photosynthesis, carry out industrialization cultivation "snakelike " photo-bioreactor system.
Background technology
The growth and breeding of photosynthetic microorganism, especially rich oil energy microalgae, Haematocoocus Pluvialls, green alga, blue-green algae etc. (being called for short algae) mainly relies on corresponding mineral substance.Single celled algae has extremely strong fecundity, can absorb a large amount of carbonic acid gas under photosynthesis.Algae by photosynthesis, utilizes the cycling of elements such as luminous energy, water, mineral substance and carbonic acid gas to become to be rich in a large amount of grease, protein, astaxanthin, the contour attached value organic compound of spirulina.
Cultivate different alga microbials by bioreactor, multiple different high value added product can be gathered in the crops.And because algae has the fast nutritive substance such as absorbing carbon dioxide, nitrogen, phosphorus be translated into the ability of organic compound (as grease, albumen, astaxanthin of being stored in cell) from surrounding environment, it can also be used for purifying liquid waste and recycle the rich carbonated stack gas of thermal power plant and carry out environmental protect.Algae can play the integral part in biopurification process in systems in which, after treating maturation, through system process such as extractions, output large number of biological fuel (as biofuel, alcohol and methane etc.), its frond also can be used as the important source material of animal-feed and fertilizer and improvement soil.In algal culture, its economic benefit produced is decided by the scale of the selection of algae, cultivation, the application efficiency of bioreactor.
Domestic at present have the bioreactor of scale to be generally open pond, transverse tube, transparent light film etc., builds up 1000 ~ 5000 square metres of annular shallow ponds (depth of water 15 ~ 30 centimetres) more, with manpower or paddle wheel, nutrient solution is circulated.This production model has significant limitation, its structure is simple, although there is the advantage of investing low easy construction, but same efficiency is too low, algae grows out of doors a lot of uncontrollable factor, lacks temperature, illumination, mixing is uneven, volume is little, contact be easy to microbiological contamination with air as open pool cultivated, make overall productivity low, often cause cultivating unsuccessfully.
Open defect has promoted the development of closed culture systems, has occurred with the closed photo bioreactor that transparent transverse tube or other container are made.The application of transverse tube type reactor is also subject to its high cost, inefficient restriction, only for the production of short run, high added value Special Products; The bioreactor of other kind also because of many reasons as extracted difficulty, construction cost is high, volume is little, cleaning is difficult etc. and in succession cannot produce economic benefit by large-scale farming.
Patent document (publication number WO 2008/151376A1) discloses a kind of film reactor, but this film reactor also exists following shortcoming:
The structural unit size of a, reactor cannot adjust after determining;
B, manufacture craft are comparatively complicated, and each film unit side is wavy, need hot pick or other auxiliary processes;
C, because reactor width is less, simultaneously wavy sidewalls causes internal run-through poor, has dead angle, cleans very inconvenient;
D, because this reactor side is main sensitive surface, reactor pose pattern (direction) is restricted;
E, reactor are comparatively dark, and the stress distribution on side plate is uneven, and material top mechanical property utilization ratio is low.Meanwhile, the material performance requirement of hydraulic pressure to membrane wall is higher.Material cost increases.
Summary of the invention
An object of the present invention is to provide one " snakelike " photo-bioreactor system.
Two of object of the present invention is to provide one " snakelike " bioreactor.
These and other objects of the present invention will be come to explain further by the following detailed description and explanation.
Of the present invention "snakelike " photo-bioreactor system, comprise process tank, detector, process pump, bioreactor, transfer line, oil return menifold, results storage tank, wherein: pass through transfer line, process tank is connected with bioreactor by process pump, results storage tank is entered by oil return menifold to harvest mouth from bioreactor product out, enter process tank from results storage tank liquid out by ultra-violet sterilization to carry out nutrient and give back, process tank is entered by head space circulation from the carbonic acid gas of harvest mouth gas out and interpolation, the amount that process tank enters bioreactor is controlled by detector.
In the present invention, said bioreactor is " snakelike ", with snakelike arrangement mode, row with to comprise multiple pipeline in the ranks and be all connected in the serpentine path that straight line extends, comprise transparent cylindrical pipe and bracing frame, cylindrical pipe is connected with bracing frame, is interconnected between bracing frame, and in 90 ° with ground; The upper and lower of two cylindrical pipes is connected by " U " type web member, the bottom of " U " type web member or top are supported each " U " pipe be fixed up by bottom or top joist steel, form " snakelike " bioreactor by many cylindrical pipes after " U " type web member connects.
In the present invention, have the rubber ring preventing from revealing, fixed by buckle between cylindrical pipe and " U " type web member, opening is arranged at bottom described " U " type web member or top, and the upper opening of each " U " type web member is all connected with vapor pipe.Multiple " snakelike " bioreactor is interconnected " snakelike " bioreactor unit of a formation standard, " snakelike " bioreactor unit of multiple standard is interconnected formation " snakelike " bioreactor, except junction, it adopts the material of whole printing opacity, and described " snakelike " bioreactor has import, outlet and thief hole.With support frame supports outside two long side surfaces of " snakelike " pipe, support frame is evenly laid along " snakelike " pipe, and the height of support frame is not less than the height of " snakelike " pipe; Support frame vertical ground, the rectangular rectangle in every side, the rectangular vertical edges in right angle is support frame column, is " snakelike " pipe between support frame column." snakelike " bioreactor structure is clearly demarcated, and each unit has the ability of certain scale and the micro-algae of cultured continuously, and the expense produced in independent operating is low.Described bioreactor is made up of 20-500000 cylindrical pipe and U-shaped pipe, be preferably and be made up of 500-50000 cylindrical pipe and U-shaped pipe, the height of cylindrical pipe and U-shaped pipe is 1.25 meters-4.20 meters, diameter is 0.07 meter-0.30 meter, is specifically determined by the kind difference of cultivating microalgae.Cylindrical pipe is made up of polyethylene terephthalate, the velocity of flow of photo bio nutrient solution is 10-20 cel, each photo bio nutrient solution residence time by " snakelike " bioreactor transparent pipeline is 10-20 minute, it within 1 to 2 hour, is a loop cycle, partly stop half operation interval, cultivate in liquid pool the nutritive substance supplemented required for photosynthetic microorganism growth in photo bio, and remove objectionable impurities.
In " snakelike " of the present invention photo-bioreactor system, by process pump, photo bio nutrient solution is squeezed into bioreactor, photosynthetic microorganism is fully absorbed, blend tank is furnished with in the front end of photo-bioreactor system device, first in blend tank the nutritive medium of micro-algae seed, plant-growth, the saturated lysate of carbonic acid gas pumps into bioreactor together, allows micro-algae plant carry out photosynthesis; Supplement the nutritive medium of micro-algae plant-growth in time according to Testing index in blend tank and supplement carbonic acid gas always, remain the saturated lysate of carbonic acid gas, keep the optimum growh state of micro-algae biology; The circulation repeatedly in bioreactor of photo bio nutrient solution supplements, flow in unit successively, from the initial beginning, flowing out to the last recycle system flows in photo bio cultivation liquid pool again, realize the stirring cycling and reutilization of photo bio nutrient solution, when reaching setting density, collector can be collected automatically, obtains both culturing microalgae product.
In " snakelike " of the present invention photo-bioreactor system, photo bio nutrient solution is introduced into cylindrical pipe again to oil return manifold by process pump, oil return manifold is by the flow set out of each pipeline and turn back to charging stock tank, when being back to the top of charging stock tank, by T-shaped tube fittings 11(Fig. 3) formed suction flow to be under, introduce gas and enter system, carbonic acid gas enters blend tank with the velocity of flow same with phegma along with phegma, the mixing that two-phase lubrication natural energy is extraordinary and mass transfer, thus in growth promoting effects medium, dissolve consumption carbonic acid gas.The suction side returning pipe arrangement is connected to the intake manifold of automatically controlled valve, and when the pH value of slurry is higher than setting point, control valve (A) is connected to CO 2 raw material gas and opens, and allows gas to be introduced in feed chute; When pH value is lower than setting point, air intake control valve is closed, and another control valve (B) is opened, and air is recycled at headspace, and exhaust system prevents carbonic acid gas at headspace; 3rd control valve (C) adds system to as safety valve and prevents nutriment tank from becoming pressurization flue gas, and in this fashion, carbonic acid gas is consumed by algae, and extra carbonic acid gas automatic input system, to maintain the pH value of action required.According to the pH value injecting carbon dioxide of system, when carbon dioxide injection is to reactor, carbonic acid will be formed, then the pH value of algae is reduced, when micro-algae is biological, after photosynthesis, the carbonic acid gas of dissolving is consumed, and the pH value of system rises, this mode can make the pH value of system remain on the value range of the most applicable algal grown, and enough carbonic acid gas can be provided again to maintain growth simultaneously.
In " snakelike " of the present invention photo-bioreactor system, the fluxion in process tank and cylindrical pipe is measured with probe a series of on detector, carbon dioxide content is in the gas phase followed the tracks of by CO2 sensors, and with the CO2 sensors dissolved to guarantee that system has enough carbonic acid gas to go to guide the growth of algae, photosynthetically active radiation can use quantum sensor, measurement of photon quantizes at photosynthetic spectrum flux, dissolved oxygen sensor is used to the product of the photosynthetic response of following the tracks of, and guarantees do not have excessive oxygen dissolution in systems in which.Algae results are by harvest mouth to an independent collection box, from concentrating algae by standard manner here, as flocculation, precipitation, centrifugation and flotation etc.Photo bio nutrient solution in systems in which circulation repeatedly supplements, and flows successively in each square formation, from the initial beginning, flows out and again flows into photo bio and cultivate in liquid pool, realize the stirring cycling and reutilization of photo bio nutrient solution to the last recycle system.When reaching setting density, collector can be collected automatically, obtains both culturing microalgae product.
" snakelike " of the present invention bioreactor overcomes that product in prior art lacks temperature, illumination, mixing is uneven, volume is little, easy microbiological contamination, difficult in maintenance, extract difficulty and wait almost all other bioreactor produced problem, achieve automatization closed culture." snakelike " bioreactor structure is clearly demarcated, and each unit has the ability of certain scale and the micro-algae of cultured continuously, and the expense produced in independent operating is low.
" snakelike " of the present invention bioreactor is when extensive industrialization, and floor space is very large, and the increase of volume can increase cost of manufacture and the difficulty of reactor; The each square formation of " snakelike " bioreactor is that multiple unit carries out being connected forming, and along with the large small-scale change in soil, successively can extend operation in batches.Reactor dismounting is simple, and convenient transport, can build in the environment of any illumination abundance, after having built, when operation and maintenance is proper, work-ing life is very long.
There are not corner angle in " snakelike " of the present invention bioreactor, the arrangement mode of connection of its " U " type, the algae in closed system can be allowed there is no the dead angle of stationary state, this makes to clean, sterilize the more convenient of change, the stirring of bioreactor culture liquid is more abundant and be evenly distributed, thus substantially increase culture efficiency, and along with height increase also can not increase cleaning difficulty.In operational process, consume the little expense of the energy low, and except " U " type junction whole printing opacity.
" snakelike " of the present invention bioreactor, solve and overcome that cleaning dead angle that existing reactor also exists, glass wall pressure, height for reactor are limited, the mobile some problem such as inconvenient of assembling, thus the photosynthetic microorganism toxigenic capacity caused is high, efficiency is low, cannot on a large scale industrialization cultivation problem.
Accompanying drawing explanation
Fig. 1 is the present invention's " snakelike " bioreactor schematic diagram.
Fig. 2 is the present invention's " snakelike " bioreactor process flow sheet.
Fig. 3 is the present invention's " snakelike " bioreactor operation chart.
Fig. 4 is " snakelike " pipe assembly schematic diagram in the present invention's " snakelike " bioreactor.
Fig. 5 is pH value determination and set-point schematic diagram.
Fig. 6 is dissolved oxygen related production and PAR schematic diagram.
In FIG, symbol 1 represents outlet, and symbol 2 represents import.
In figs. 2 and 3, symbol 1 represents process tank, symbol 2 represents detector, symbol 3 represents process pump, symbol 4 represents loop shunting, and symbol 5 represents transfer line, and symbol 6 represents phototube row, symbol 7 represents oil return menifold, symbol 8 represents harvest mouth, and symbol 9 represents gas entrainment, and symbol 10 represents head space circulation, symbol 11 represents carbonic acid gas to be added, symbol 12 representative returns process tank, and symbol 13 represents results storage tank, and symbol 14 represents nutrient and gives back, symbol 15 represents ultra-violet sterilization, and symbol 16 represents removes concentrated biomass.
In figure 3, symbol A, B, C represents control valve.
With reference to figure 2, photo bio nutrient solution is incorporated into PET pipe by manifold out by impeller pump.Generally remain in the scope of 10-20 cel by the velocity of flow of each pipe, each photo bio nutrient solution by PBR bioreactor transparent pipeline provides the residence time of about 10-20 minute.At the end of PBR, to combine common flowing turn back to process tank at each parallel lines of manifold.When being back to the top of process tank, being formed under suction flows to and be, introduce gas simultaneously and enter system by T-shaped tube fittings, carbonic acid gas enters process tank along with the velocity of flow that phegma is same with phegma.The mixing that two-phase lubrication natural energy is extraordinary and mass transfer, thus in growth promoting effects medium, dissolve consumption carbonic acid gas.Use such system to utilize the Infrastructure of pipeline and impeller pump extremely to put in place, can well produce a desired effect.
With reference to figure 3, describe nutriment tank and associated conduit comprises return line.The suction side returning pipe arrangement is connected to the intake manifold of automatically controlled valve.Open when the pH value of slurry is connected to CO 2 raw material gas higher than setting point (such as pH 7.0) control valve (A), allow gas to be introduced in feed chute.When pH value is lower than setting point, air intake control valve is closed, and another control valve (B) is opened, and air is recycled at headspace, and exhaust system prevents carbonic acid gas at headspace.3rd control valve (C) adds system to as safety valve and prevents nutriment tank from becoming pressurization flue gas.In this fashion, carbonic acid gas is consumed by algae, and extra carbonic acid gas automatic input system, to maintain the pH value of action required.
With reference to figure 4, the selection of material and chain joint assembly: thin-walled PET manages, commonly use the material into packaging, form the main body of bioreactor with US spec 40 polyvinyl chloride tube fittings, transverse tube is above the vapor pipe under system quiescence state; To be " U " type pipe be here between series unit is connected in parallel mouth.Water-tight in order to ensure sealing, the rubber ring of suitable size is used as creating the pad between pipeline and accessory.A worm drive clip is used to reinforce sealing.Vertical ducting is by prefabricated joist steel framework, and bottom joist steel supports each " U " pipe be fixed up, the stability of keeping system; Top is also fixed by joist steel, instead of is suspended on top.The weight of water can be made to become the force of compression of reactor from bottom support reactor, thus keep together, instead of attempt to tear up it.
With reference to figure 5, according to the pH value injecting carbon dioxide of system.When carbon dioxide injection is to reactor, carbonic acid (carbon dioxide solubility generates carbonic acid in water) will be formed, then reduce the pH value of algae.When micro-algae is biological, after photosynthesis, the carbonic acid gas of dissolving is consumed, and the pH value of system rises.This mode can make the pH value of system remain on the value range (pH=4.2-6.2) of the most applicable algal grown, and enough carbonic acid gas can be provided again to maintain growth simultaneously.This point ten minutes important, especially in nutriment gas containing other acidic flue gas compositions as SOx and NOx.Especially the lysate of SOx can form sulfuric acid, makes substratum peracid, thus Developing restraint.For this reason, SOx will not be added into the culture systems of algae utilization.
Fig. 5 describes the way of control ph, for regulating the carbon dioxide flow of the marine alga of 650 liters of pipelines in six days.Sea line is the pH setting point representing reactor, and trace line represents is the pH value of systematic survey.This figure also captures the generation of the breathing producing carbonic acid gas, thus is reduced in the pH value at night.
With reference to figure 6, except pH value, also have much different modes constantly to monitor in the middle of operational process and follow the tracks of the performance of bioreactor and the health of algae.These metering systems (comprising pH value) are by the fluxion between a series of probe monitoring nutriment tank and PBR.Environment and the temperature of process are all through the system performance measurement relevant with environmental influence.Carbon dioxide content is in the gas phase followed the tracks of by CO2 sensors, and with the CO2 sensors dissolved to guarantee that system has enough carbonic acid gas to go to guide the growth of algae.If needed, photosynthetically active radiation (PAR) can use quantum sensor, and measurement of photon quantizes at photosynthetic spectrum flux.Dissolved oxygen sensor is used to the product of the photosynthetic response of following the tracks of, and guarantees do not have excessive oxygen dissolution in systems in which.The generation of oxygen is equal to the consumption of carbonic acid gas.Therefore, by monitoring dissolved oxygen, can confirm that the performance of algae is with healthy.Fig. 6 shows the relation of dissolved oxygen in bioreactor and PAR value.
It should be noted that oxygen inhibiting rate can by the productivity of the ability interference restriction algae of the basal component absorbing carbon dioxide in alga cells.But when using the carbon dioxide source of stack gas, problem just becomes very little, because carbonic acid gas is as the gas after burning, it is compared to the oxygen level of little air 80%.

Claims (12)

1. one kind "snakelike " photo-bioreactor system, it is characterized in that comprising process tank, detector, process pump, bioreactor, transfer line, oil return menifold, results storage tank, wherein: pass through transfer line, process tank is connected with bioreactor by process pump, results storage tank is entered by oil return menifold to harvest mouth from bioreactor product out, enter process tank from results storage tank liquid out by ultra-violet sterilization to carry out nutrient and give back, process tank is entered by head space circulation from the carbonic acid gas of harvest mouth gas out and interpolation, the amount that process tank enters bioreactor is controlled by detector.
2. according to claim 1 said " snakelike " photo-bioreactor system, it is characterized in that said bioreactor is for " snakelike ", with snakelike arrangement mode, row with to comprise multiple pipeline in the ranks and be all connected in the serpentine path that straight line extends, comprise transparent cylindrical pipe and bracing frame, cylindrical pipe is connected with bracing frame, is interconnected between bracing frame, and in 90 ° with ground; The upper and lower of two cylindrical pipes is connected by " U " type web member, the bottom of " U " type web member or top are supported each " U " pipe be fixed up by bottom or top joist steel, form " snakelike " bioreactor by many cylindrical pipes after " U " type web member connects.
3. according to claim 2 said " snakelike " photo-bioreactor system, it is characterized in that there is the rubber ring preventing from revealing between cylindrical pipe and " U " type web member, fixed by buckle, opening is arranged at bottom described " U " type web member or top, and the upper opening of each " U " type web member is all connected with vapor pipe.
4. according to claim 2 said " snakelike " photo-bioreactor system, it is characterized in that multiple " snakelike " bioreactor is interconnected " snakelike " bioreactor unit of a formation standard, " snakelike " bioreactor unit of multiple standard is interconnected formation " snakelike " bioreactor, and described " snakelike " bioreactor has import, outlet and thief hole.
5. according to one of claim 2-4 said " snakelike " photo-bioreactor system, it is characterized in that described bioreactor is made up of 20-500000 cylindrical pipe and U-shaped pipe, be preferably and be made up of 500-50000 cylindrical pipe and U-shaped pipe, the height of cylindrical pipe and U-shaped pipe is 1.25 meters-4.20 meters, and diameter is 0.07 meter-0.30 meter.
6. according to one of claim 1-5 said " snakelike " photo-bioreactor system, it is characterized in that cylindrical pipe is made up of polyethylene terephthalate, the velocity of flow of photo bio nutrient solution is 10-20 cel, each photo bio nutrient solution residence time by " snakelike " bioreactor transparent pipeline is 10-20 minute, it within 1 to 2 hour, is a loop cycle, partly stop half operation interval, cultivate in liquid pool the nutritive substance supplemented required for photosynthetic microorganism growth in photo bio, and remove objectionable impurities.
7. according to one of claim 1-5 said " snakelike " photo-bioreactor system, it is characterized in that, by process pump, photo bio nutrient solution is squeezed into bioreactor, photosynthetic microorganism is fully absorbed, blend tank is furnished with in the front end of photo-bioreactor system device, first in blend tank the nutritive medium of micro-algae seed, plant-growth, the saturated lysate of carbonic acid gas pumps into bioreactor together, allows micro-algae plant carry out photosynthesis; Supplement the nutritive medium of micro-algae plant-growth in time according to Testing index in blend tank and supplement carbonic acid gas always, remain the saturated lysate of carbonic acid gas, keep the optimum growh state of micro-algae biology; The circulation repeatedly in bioreactor of photo bio nutrient solution supplements, flow in unit successively, from the initial beginning, flowing out to the last recycle system flows in photo bio cultivation liquid pool again, realize the stirring cycling and reutilization of photo bio nutrient solution, when reaching setting density, collector can be collected automatically, obtains both culturing microalgae product.
8. according to claim 1 or 7 said " snakelike " photo-bioreactor system, it is characterized in that photo bio nutrient solution is introduced into cylindrical pipe again to oil return manifold by process pump, oil return manifold is by the flow set out of each pipeline and turn back to charging stock tank, when being back to the top of charging stock tank, by T-shaped tube fittings 11 formed suction flow to be under, introduce gas and enter system, carbonic acid gas enters blend tank with the velocity of flow same with phegma along with phegma, the mixing that two-phase lubrication natural energy is extraordinary and mass transfer, thus in growth promoting effects medium, dissolve consumption carbonic acid gas.
9. according to claim 1 or 7 said " snakelike " photo-bioreactor system, the suction side that it is characterized in that returning pipe arrangement is connected to the intake manifold of automatically controlled valve, when the pH value of slurry is higher than setting point, control valve (A) is connected to CO 2 raw material gas and opens, and allows gas to be introduced in feed chute; When pH value is lower than setting point, air intake control valve is closed, and another control valve (B) is opened, and air is recycled at headspace, and exhaust system prevents carbonic acid gas at headspace; 3rd control valve (C) adds system to as safety valve and prevents nutriment tank from becoming pressurization flue gas, and in this fashion, carbonic acid gas is consumed by algae, and extra carbonic acid gas automatic input system, to maintain the pH value of action required.
10. according to claim 1 or 7 said " snakelike " photo-bioreactor system, it is characterized in that the pH value injecting carbon dioxide according to system, when carbon dioxide injection is to reactor, will carbonic acid be formed, then reduce the pH value of algae, when micro-algae is biological after photosynthesis, the carbonic acid gas dissolved is consumed, the pH value of system rises, and this mode can make the pH value of system remain on the value range of the most applicable algal grown, and enough carbonic acid gas can be provided again to maintain growth simultaneously.
11. according to claim 1 or 7 said " snakelike " photo-bioreactor system, it is characterized in that the fluxion measured with probe a series of on detector in process tank and cylindrical pipe, carbon dioxide content is in the gas phase followed the tracks of by CO2 sensors, and with the CO2 sensors dissolved to guarantee that system has enough carbonic acid gas to go to guide the growth of algae, photosynthetically active radiation can use quantum sensor, measurement of photon quantizes at photosynthetic spectrum flux, dissolved oxygen sensor is used to the product of the photosynthetic response of following the tracks of, guarantee there is no excessive oxygen dissolution in systems in which.
12. according to claim 1 or 7 said " snakelike " photo-bioreactor system, it is characterized in that algae results are by harvest mouth to an independent collection box, from concentrating algae by standard manner here, as flocculation, precipitation, centrifugation, flotation etc.
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Cited By (5)

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CN105039134A (en) * 2015-05-28 2015-11-11 连衡会投资有限公司 Circular flow type photobioreactor system
CN107012082A (en) * 2017-04-25 2017-08-04 北海生巴达生物科技有限公司 The bioreactor of both culturing microalgae
US20170355942A1 (en) * 2016-06-13 2017-12-14 University Of Kentucky Research Foundation Cyclic photobioreactor and method for biofilm control
CN109589906A (en) * 2018-12-28 2019-04-09 孙志良 A kind of coil pipe type reactor suitable for liquid-phase chemical reaction
CN110656105A (en) * 2019-11-12 2020-01-07 宁波大学 Circulating flow type ultraviolet ray stress microalgae culture method

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