WO2016187996A1

WO2016187996A1 - Circular flow type photobioreactor system

Info

Publication number: WO2016187996A1
Application number: PCT/CN2015/090215
Authority: WO
Inventors: 迈克尔·海因斯·威尔逊; 约翰·格洛波; 托马斯·格拉布斯; 查尔斯·塞西尔; 马克·克罗克; 托马斯·刘; 张项羽; 张恒; 刘俊国
Original assignee: 连衡会投资有限公司
Priority date: 2015-05-28
Filing date: 2015-09-22
Publication date: 2016-12-01
Also published as: CN105039134B; CN105039134A

Abstract

Provided is a circular flow type photobioreactor system, comprising a middle storage tank 1, a liquid operation pump 2, a recycling/drain return pipeline 3, a recycling/return pipe 4, an overflowing liquid/gas return pipe 5, a photobioreactor 6, a receiving hole 7, a receiving storage tank 8, a waste liquid/waste nutrient solution 9, an ultraviolet sterilization device 10, a concentrated biomass product 11, a returning pipe 12, a flue gas/carbon dioxide introducing device 13, a gas operation pump 14, a flue and a pH value and dissolved oxygen probe 15. With the circular flow type photobioreactor system, the automatic closed type culture is realized.

Description

Circulating flow photobioreactor system

Technical field

The invention relates to a closed photobioreactor system, in particular to a circulating flow photobioreactor system for cultivating a plurality of photosynthetic microalgae organisms by photosynthesis for industrial microalgae cultivation.

Background technique

Photosynthetic microalgae organisms, especially oil-rich microalgae, plastic microalgae, Haematococcus pluvialis, green algae, cyanobacteria, algae, etc. (abbreviated as algae) rely mainly on the corresponding minerals. Single-celled algae have a strong ability to reproduce and absorb a large amount of carbon dioxide under photosynthesis. Through photosynthesis, algae are converted into high-value organic compounds rich in oil, protein, astaxanthin, and antioxidants by using elements such as light energy, water, minerals, and carbon dioxide.

A variety of high value-added products can be harvested by culturing different algal microorganisms in a photobioreactor. And because algae has the ability to rapidly absorb nutrients such as carbon dioxide, nitrogen, and phosphorus from the surrounding environment and convert it into organic compounds such as oils, proteins, and astaxanthin stored in cells, it can also be used to purify wastewater. And recycle the carbon dioxide-rich flue gas from thermal power plants to improve the environment. In the system, algae can play an important part in the biological purification process. After being matured, through systematic processes such as collection and extraction, a large amount of biofuels (such as biodiesel, alcohol and methane) can be produced, and the algae can also be used as animals. Feed and organic fertilizers, as well as important raw materials for improving the soil. In algae aquaculture, the economic benefits are determined by the choice of algae, the scale of the culture, the application efficiency of the photobioreactor design, and the cost of application.

At present, China's large-scale photobioreactors are generally open pools, horizontal pipes, pipes, transparent light films, plastic films, etc., and open pools are built with 1000-5000 square meters of circular shallow pools (water depth). 15 to 30 cm), the culture solution is circulated by human or slurry wheel. This production mode has great limitations. Its structure is simple. Although it has the advantages of low investment and easy construction, the same efficiency is too low. There are many uncontrollable factors for algae growing outdoors. For example, open pool farming lacks temperature, illumination, The mixing is uneven, the volume is small, and the contact with the air is easy to be infected, which makes the overall productivity low, often leading to failure of culture.

Open defects have driven the development of closed culture systems, with closed photobioreactors made of transparent pipes, plates or other containers. The application of horizontal tube and flat reactors is also limited by their high cost and low efficiency. It is only used to produce small batches and high value-added special products. Other types of photobioreactors are difficult to extract due to various reasons. The high construction cost, small volume, difficulty in cleaning, etc., and the inability to large-scale aquaculture have produced economic benefits, limiting the scale of development of the microalgae industry.

A thin film reactor is disclosed in the patent document (Publication No. WO 2008/151376 A1), however, the thin film reactor also has the following disadvantages:

a, the structural unit size of the reactor can not be adjusted after determining;

b, the production process is more complicated, each side of the film unit is wavy, requiring hot joining or other auxiliary processes;

c. Because the reactor width is small, and the wavy sidewalls cause poor internal penetration, and there is a dead angle, cleaning is inconvenient;

d. Since the side of the reactor is the main light receiving surface, the reactor placement form (direction) is limited;

e. The reactor is deep, the stress distribution on the side plates is uneven, and the mechanical properties of the upper part of the material are low. At the same time, the water pressure requires high material properties of the film wall. The cost of materials increases.

Summary of the invention

One of the objects of the present invention is to provide a circulating flow photobioreactor system.

Another object of the present invention is to provide a circulating flow photobioreactor and a circulating flow photobioreactor unit.

A third object of the present invention is to provide a pig which can automatically clean the inner wall of a transparent pipe.

These and other objects of the present invention will be further clarified by the following detailed description and description.

Summary of the invention

The circulating flow photobioreactor system of the present invention comprises an intermediate storage tank 1, a liquid operation pump 2, a recovery/drainage return pipeline 3, a recovery/return tube 4, an overflow/liquid, a gas return tube 5, and a photobioreactor 6 Harvesting port 7, harvesting storage tank 8, waste liquid/waste nutrient solution 9, ultraviolet sterilizing device 10, concentrated biomass product 11, return pipe 12, flue gas/carbon dioxide introducing device 13, gas running pump 14, flue And pH and dissolved oxygen probe 15. The nutrient solution prepared in the middle is placed in the intermediate storage tank 1, and the nutrient solution is transported to the lower portion of the photobioreactor 6 through the liquid operation pump 2, and the flue gas/carbon dioxide is introduced into the photobio by the gas operation pump 14. The microalgae in the reactor is subjected to photosynthesis. After the photosynthesis is completed, the cultured microalgae density saturated liquid passes through the recovery/drainage returning pipe 3, passes through the recovery/return pipe 4 under the action of the liquid running pump 2, and enters through the harvesting port 7 In the harvesting tank 8, the liquid-solid separation removes the concentrated microalgae biomass product 11, and the remaining waste liquid/waste nutrient solution 9 is sterilized by the ultraviolet device 10 and returned to the intermediate storage tank 1 for reuse after nutrient preparation. The gas and liquid overflowing from the upper portion of the photobioreactor 6 are returned to the intermediate storage tank 1 through the overflow/liquid and gas return pipe 5 via the return pipe 12, and are used for nutrient preparation.

In the circulating flow photobioreactor system of the present invention, the photobioreactor 6 is composed of a plurality of transparent cylindrical transparent pipes arranged side by side, and the upper and lower portions of the cylindrical transparent pipe are connected through a three-way connection, each group The photobioreactor unit consists of two photobioreactors 6 staggered at 45°. The upper and lower portions of the two photobioreactors 6 are interconnected to form an inlet and an outlet, said transparent cylindrical transparent tubes and connections. The tee is made of polyethylene terephthalate.

In the circulating flow photobioreactor system of the present invention, a gas delivery line is disposed in the lower communication tube of the photobioreactor 6, and the gas delivery line is densely packed for setting a certain pressure. The small hole in which the gas escapes.

In the circulating flow photobioreactor system of the present invention, a pig is built in each of the cylindrical transparent tubes of the photobioreactor 6. Each of the pigs includes: a foam-filled cylinder, a gasket, two rubber seals, two notched disc rubber gaskets and two porous discs; the pig is foamed The upper and lower sides of the cylinder are sequentially connected with a rubber sealing ring, a disc rubber gasket having a notch, a gasket, and a disk having a porous shape, which are connected by a screw and a nut. The notched disc rubber gasket has several notches for allowing sufficient water to flow. The angle between the upper and lower notches through which the upper and lower rubber gaskets are reserved is 45 degrees, and the gap between the upper and lower rubber gaskets is 4 The pig is rotated by the water flow force through which the water passes to ensure that the inner wall of the transparent pipe seamlessly cleans all of its area.

In the circulating flow photobioreactor system of the present invention, the lower part and the upper part of the photobioreactor unit are supported by the three-way pipe and the plurality of circulating flow pipes by the I-beam and are fixed by the buckle and the ground. At 90°, there is a rubber ring between the connecting cylindrical transparent pipe and the pipe tee to prevent leakage, which is fixed by a buckle.

In the circulating flow photobioreactor system of the present invention, a plurality of photobioreactors The unit is connected in series, arranged in parallel with two rows of units, and the end-to-end connection extends to form a circulating flow photobioreactor. The plurality of circulating flow photobioreactors are connected in parallel to form a circulating flow photobioreactor system. . The photobioreactor comprises: 20-500,000 cylindrical transparent pipes and pipe tees, I-beams, connecting pieces, support frames and fixed components, and the photobioreactor units are connected in series, in two rows, in parallel, in series Together, the end-to-end connection extends to form a circulating flow photobioreactor; a plurality of circulating flow photobioreactors are then connected in parallel to form a circulating flow photobioreactor system. The cylindrical transparent pipe has a height of 0.80 m to 6.00 m and a diameter of 0.05 m to 0.55 m.

In the circulating flow photobioreactor system of the present invention, after the microalgae growth liquid is prepared in the mixing tank, the flow velocity is 10-20 cm/sec, and the circulatory flow photobioreactor system is introduced, and the microalgae grows. The liquid stays in each cylindrical transparent pipe, uniformly passes carbon dioxide, carries out photosynthesis, and the residence time of the microalgae growth liquid is about 8-16 hours according to the microalgae seed. When the detector detects that the microalgae liquid is insufficiently nutritious, the microalgae liquid is returned to the culture solution by the inlet and outlet to prepare the storage tank, and the nutrient required for the growth of the appropriate amount of microalgae is added; and the next microalgae photobioreaction cycle is entered.

The microalgae growth liquid is driven into the photobioreactor by running the pump to fully absorb the photosynthetic microalgae organism, and the carbon biogas storage tank, the distribution storage tank, the harvest storage tank are equipped at the front end of the photobioreactor system device, and the first time in the deployment In the tank, the microalgae seeds and the nutrient solution for plant growth are pumped together into the photobioreactor system, and then the carbon dioxide control valve that opens into the cylindrical pipe is opened, and the carbon dioxide is added to keep the carbon dioxide in the pH setting range of the nutrient solution. Let the microalgae plants maintain the optimal growth state for photosynthesis; according to the detection index, the microalgae plant growth liquid is returned to the mixing tank to supplement the nutrient growth of the microalgae plant. When the microalgae liquid reaches the set density, the microalgae liquid Enter the harvest storage tank and collect it automatically by the collector (liquid-solid separator) to obtain microalgae culture products.

The microalgae growth liquid enters the circulating flow photobioreactor system, and the microalgae growth liquid stays in each of the cylindrical transparent pipes, and the inlet end of the inlet of the pipe tee is connected to the intake manifold of the automatic control valve, and is opened. The carbon dioxide control valve that passes into the cylindrical transparent pipe always supplements the carbon dioxide. When the pH of the slurry is higher or lower than the set range, the gas control valve adjusts to increase or decrease the amount of carbon dioxide replenishment within the pH setting range. Maintaining the most appropriate solubility of carbon dioxide, allowing microalgae plants to maintain optimal growth for photosynthesis.

A series of probes on the flue and pH and dissolved oxygen probe 15 are used to determine the density of liquid microalgae in the intermediate storage tank, the mixing tank and the cylindrical transparent tube. The carbon dioxide content in the gas phase is tracked by the carbon dioxide sensor. To ensure that the system has enough carbon dioxide to guide the growth of algae, photosynthetically active radiation can be quantified using photosensors, photon fluxes are quantified in the photosynthetic flux, and dissolved oxygen sensors are used to track the photosynthetic reaction, ensuring that there is no excess in the system. Oxygen is dissolved.

In the circulating flow photobioreactor system of the present invention, algae harvesting is carried out when the set density is reached, and the microalgae liquid enters the harvesting tank from the inlet and outlet, from which algae can be collected by standard means: flocculation, sedimentation, centrifugation Separation, flotation, etc. are collected by a collector to obtain a microalgae culture product.

In the circulating flow photobioreactor system of the present invention, the circulating flow photobioreactor comprises a gas, a liquid detector, a sensor, a running liquid pump, an air pump, an intermediate storage tank, a liquid solid separator, a photobio Reactors and components (cylindrical transparent pipes and pipe tees, support frames, connectors, etc.), liquid transfer lines, carbon dioxide gas transfer circulation lines and carbon dioxide storage tanks, liquid return manifolds, blending tanks, harvesting storage a tank, wherein: the intermediate storage tank, the preparation storage tank and the photobioreactor system are connected through a conveying pipeline, a running pump, and the cultured microalgae high-density liquid from the photobioreactor system is passed through the liquid returning pipe to the harvesting port. Harvesting tank; flocculation, liquid solid The solids from the separator are microalgae products for reprocessing. The waste liquid enters the blending tank through the intermediate storage tank and ultraviolet sterilization, and then the seeds, nutrients, etc. are added and returned, and the amount of the photobioreactor system is controlled by the detector. The carbon dioxide exhaust gas discharged from the photobioreactor system and the gas and liquid overflowing from the upper portion of the photobioreactor 6 are returned to the intermediate storage tank 1 through the overflow/liquid, gas return pipe 5 through the return pipe 12, and are used after re-nutrition preparation. The amount of carbon dioxide entering the photobioreactor system is controlled by a gas detector through a carbon dioxide gas delivery loop.

In the circulating flow photobioreactor system of the present invention, the cycling flow photobioreactor material selection and linking components: thin-walled PET tubes, commonly used as packaging materials, and American size 40 polyvinyl chloride tubes The accessory constitutes the main body of the photobioreactor, the thinner cross tube on the upper part is the exhaust pipe in the system static state; the lower part is the cylindrical pipe tee, the cylindrical transparent pipe, and the lower part or the upper part of the connecting piece is made of the I-beam The three-way supports and fixes a plurality of circulating flow pipes, and the circulating flow photobioreactor units are arranged in series, in parallel, and in two rows, and the first and last connections are extended. To ensure that the seal does not leak, a properly sized rubber band is used to create the gasket between the pipe and the fitting, and a worm drive clamp is used to reinforce the seal. The vertical pipe is made of prefabricated I-beam steel frame. The bottom I-beams connect the circulating flow photobioreactor unit in series with the end-to-end connection, support and fix it to maintain the stability of the system; the top is also fixed by I-beam. Instead of hanging at the top. The support frame is evenly arranged along the parallel pipe. The height of the support frame is not less than the height of the cylindrical transparent pipe and the pipe tee; the support frame is 90 degrees perpendicular to the ground, each side is rectangular at right angles, and the vertical side of the rectangular rectangle is the support frame column, overlooking Extend the connection for the first and last connections. Each of the circulating flow photobioreactor systems has a certain scale and the ability to independently culture microalgae continuously, resulting in low costs in independent operation.

In the circulating flow photobioreactor system of the present invention, the circulating flow light Each of the cylindrical transparent pipes in the bioreactor system has an automatic washer device---pipe cleaner to ensure the continuous operation of the "circulating flow" photobioreactor system, without disassembly and automatic cleaning;

The role of the pig is to prevent the formation of biofilm in the PET tube. To accomplish this task, when the pipe is filled with water, each trimmer will be raised to the top of the corresponding pipe row and must return to the bottom when the pipe row is empty. The body part of the pig is a foam-filled cylinder (Part A), large enough to make it buoyant enough to rise and fall with the water level in the tube row. As the pig rises (and descends), the edges of its two rubber seals (Part B) remove all algae that grow on the PET tube wall. However, although these shims must remain in contact with the edges of the tube rows, they must also not limit the flow of water around the pigs. To prevent such limitations, each shim should have several notches that allow sufficient water to flow through. The angle between the upper and lower gaps through which the water flows from the upper and lower rubber gaskets is 45 degrees. When the water passes, the pig is rotated under the action of the water flow force to ensure that the inner wall of the transparent pipe is cleaned seamlessly. area. Each pig also has a perforated disc (Part C) at both ends to prevent the pig from leaving the tube row while allowing the microalgae growth fluid to flow in and out of the top (and bottom) of the manifold, respectively As shown in Figure 2 and Figure 3. Finally, the entire pig fitting is made up of a combination of screw and nut (D and E sections).

In the circulating flow photobioreactor system of the present invention, the circulating flow photobioreactor overcomes the lack of temperature, illumination, uneven mixing, small volume, easy dyeing, and maintenance difficulty in the prior art products. The problem of almost all other photobioreactors, such as difficulty in extraction, has been realized, and automated closed culture has been realized. The circulating flow photobioreactor system has a well-defined structure, each unit plate has a certain scale and the ability to continuously culture microalgae, and the cost of a single circulating flow photobioreactor is low in independent operation.

In the circulating flow photobioreactor system of the present invention, the circulating flow photobioreactor system is most suitable for large scale industrialization. Each of the square matrix of the circulating flow photobioreactor system is formed by combining and connecting a plurality of units. As the size and scale of the land change, the operation can be expanded in batches. The reactor is easy to disassemble and easy to transport. It can be built in any environment with sufficient light. After the construction is completed, it has a long service life under the condition of proper operation and maintenance.

The circulating flow type photobioreactor system of the invention has no angular edges, and the arrangement of the cylindrical transparent shaped pipes can make the algae in the closed system have no dead angle of static state, which makes cleaning and disinfection more simple and convenient. The agitation of the reactor culture liquid is more sufficient and evenly distributed, thereby greatly improving the culture efficiency, the design of the independent automatic cleaning device, and the difficulty of cleaning is not increased with the increase of the height; the energy consumption is low and the cost is low during the operation. And all except the cylindrical pipe tee joint, all made of light-transmitting polyethylene terephthalate.

The circulating flow type photobioreactor system of the invention has the advantages of long service life, large capacity and low operation cost, and is most suitable for large-scale industrialization; solving the problem of overcoming the cleaning dead angle, glass wall pressure and reaction existing in the existing reactor There are several problems such as limited height of the device and inconvenient assembly and movement, resulting in high cost, low efficiency, and large-scale industrial farming of photosynthetic microalgae. It is the most suitable closed-loop circulating photobioreactor system for the industrialization of microalgae aquaculture; in particular, the volumetric capacity is 10-20 times higher than that of the conventional closed photobioreactor algae growth nutrient liquid. Formerly our company's serpentine photobioreactor system has 50% more algae growth nutrient liquid capacity.

DRAWINGS

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic flow diagram of a circulating flow photobioreactor of the present invention.

Figure 2 is a schematic view showing the assembly of the pig of the present invention.

Figure 3 is a cross-sectional view showing the state of use of the pig of the present invention in a pipe.

Figure 4 is a schematic view showing the connection of the circulating flow photobioreactor unit of the present invention.

Figure 5 is a schematic diagram of measuring pH and set points.

Figure 6 is a schematic diagram of dissolved oxygen related production and PAR.

Figure 7 is a schematic view showing the circular pipe connection of the circulating flow photobioreactor of the present invention.

In Fig. 1, symbol 1 represents an intermediate storage tank, symbol 2 represents a liquid running pump, symbol 3 represents a recovery/drainage return pipe, symbol 4 represents a recovery/return pipe, symbol 5 represents an overflow/liquid, a gas return pipe, and symbol 6 represents Photobioreactor, symbol 7 represents the harvesting port, symbol 8 represents the harvesting tank, symbol 9 represents the waste liquid/waste nutrient solution, symbol 10 represents the ultraviolet disinfection device, symbol 11 represents the concentrated biomass product, symbol 12 represents the return tube, Symbol 13 represents a flue gas/carbon dioxide introduction device, symbol 14 represents a gas running pump, and symbol 15 represents a flue and a pH value and a dissolved oxygen probe.

In Fig. 2, Fig. 3, symbol A represents a cylinder filled with foam, symbol B represents a rubber seal, symbol C represents a disc having a notch, symbol D represents a screw, and symbol E represents a nut.

In Fig. 4, symbol 16 represents photobioreactor A, symbol 17 represents photobioreactor B, symbol 18 represents a connector, and symbol 19 represents a connecting tube.

The present invention will be described in detail below with reference to the accompanying drawings.

Referring to Figure 1, the cylindrical transparent pipe and the pipe tee are made of polyethylene terephthalate, and the microalgae growth liquid is prepared in the mixing tank, and the flow rate is 10-20 cm/sec. In the flow photobioreactor system, the microalgae growth solution stays in each cylindrical transparent pipe, and uniformly passes carbon dioxide for photosynthesis; the residence time of the microalgae growth solution is about 6-12 depending on the microalgae seed. The hour is a cycle; return to the microalgae growth liquid pool to supplement the nutrients needed for the growth of photosynthetic microalgae, and to remove harmful substances.

Referring to Figure 7, the material selection and linking components: thin-walled PET tubes, commonly used as packaging materials, The main body of the photobioreactor is made up of the American specification 40 polyvinyl chloride pipe fitting. The thinner horizontal pipe on the upper part is the exhaust pipe in the static state of the system; the lower part is the cylindrical pipe tee, the cylindrical transparent pipe, the connecting piece The lower part or the upper part uses the I-beam to support and fix the plurality of circulating flow pipes in parallel, parallel, and two rows of rows, and the first and last joints extend and communicate. In order to ensure that the cylindrical transparent pipe and the pipe tee joint seal does not leak water, a properly sized rubber band is used to create a gasket between the pipe and the fitting. Use a worm drive clamp to reinforce the seal. The vertical pipe is made of prefabricated I-beam steel frame. The bottom I-beam extends the parallel connection of the head and tail connections, supports and fixes it to maintain the stability of the system; the top is also fixed by the I-beam instead of hanging at the top.

Referring to Figure 5, carbon dioxide is injected according to the pH of the system. When carbon dioxide is injected into the reactor, carbonic acid is formed (carbon dioxide is dissolved in water to form carbonic acid), which in turn reduces the pH of the algae. When the microalgae organisms are photosynthesized, the dissolved carbon dioxide is consumed and the pH of the system rises. This approach maintains the pH of the system at a range that is most suitable for algae growth (pH = 4.2-6.2) while providing sufficient carbon dioxide to sustain growth. This is important, especially if the nutrient gas contains other acidic flue gas components such as SOx and NOx. In particular, the SOx solution forms sulfuric acid, which causes the medium to be acidified, thereby inhibiting growth. For this reason, SOx will not be added to the culture system utilized by algae.

Figure 5 depicts the method of controlling the pH for adjusting the carbon dioxide flow of algae in a 650 liter pipeline over a six day period. The horizontal line represents the pH set point of the reactor and the trace line represents the pH value measured by the system. This figure also captures the occurrence of carbon dioxide-producing respiration, thereby reducing the pH at night.

Referring to Figure 6, in addition to the pH, there are many different ways to continuously monitor the performance of the photobioreactor and the health of the algae during operation. These measurements (including pH) The flowing liquid between the feed tank and the PBR is monitored by a series of probes. Both ambient and process temperatures are measured by system performance and environmental impact. The carbon dioxide content in the gas phase is tracked by a carbon dioxide detector to ensure that the system has enough carbon dioxide to guide the growth of the algae. If desired, photosynthetically active radiation (PAR) can be quantified using photosensors using photosensors. The dissolved oxygen sensor is the product of the photosynthetic reaction used to track, ensuring that no excess oxygen is dissolved in the system. The production of oxygen is equivalent to the consumption of carbon dioxide. Therefore, by monitoring dissolved oxygen, the performance and health of algae can be confirmed. Figure 6 shows the relationship between dissolved oxygen and PAR values in a photobioreactor.

It is worth noting that the oxygen inhibition rate can interfere with limiting the productivity of algae by the ability of the essential components in algae cells to absorb carbon dioxide. However, when using a source of carbon dioxide from the flue gas, the problem becomes very small because carbon dioxide, as a gas after combustion, is 80% less oxygen than air.

Claims

A circulating flow photobioreactor system, characterized by comprising an intermediate storage tank (1), a liquid running pump (2), a recovery/drainage returning pipeline (3), a recovery/return tube (4), an overflow/liquid, Gas return tube (5), photobioreactor (6), harvesting port (7), harvesting storage tank (8), waste liquid/waste nutrient solution (9), ultraviolet disinfection device (10), concentrated biomass product (11), return pipe (12), flue gas/carbon dioxide introduction device (13), gas operation pump (14), flue and pH value and dissolved oxygen probe (15); Into the intermediate storage tank (1), the nutrient solution is transported to the lower side of the photobioreactor (6) through the pump (2), and the purified flue gas/carbon dioxide is introduced into the photobioreaction through the gas running pump (14). The microalgae in the device is subjected to photosynthesis. After the photosynthesis is completed, the cultured microalgae density saturated liquid passes through the recovery/drainage return pipe (3), and passes through the recovery/return pipe (4) under the action of the liquid running pump (2). From the harvesting port (7) into the harvesting tank (8), the liquid-solid separation removes the concentrated microalgae biomass product (11), and the remaining waste liquid/waste nutrient solution (9) passes After the ultraviolet device (10) is sterilized, the waste nutrient solution is returned to the intermediate storage tank (1) for re-nutrition preparation; the gas and liquid overflowing from the upper portion of the photobioreactor (6) are passed through the overflow/liquid, gas return tube (5) It is also returned to the intermediate storage tank (1) via the return pipe (12) for re-nutrition preparation.
The circulating flow photobioreactor system according to claim 1, wherein said photobioreactor (6) has a plurality of transparent cylindrical transparent tubes formed in two rows, and an upper portion of the cylindrical transparent tubes And the lower part is connected through a three-way connection, each group of photobioreactor units is composed of two photobioreactors (6) arranged in a staggered arrangement of 45°, and the upper and lower parts of the two photobioreactors (6) are connected to each other to form a Import and export.
The circulating flow photobioreactor system according to claim 1, wherein a gas delivery line is disposed in the lower communication tube of the photobioreactor (6), and the gas delivery line is densely packed with a plurality of A small hole that escapes from the gas.
The circulating flow photobioreactor system according to claim 1, characterized in that a pig is built in each of the cylindrical transparent pipes of the photobioreactor (6).
The circulating flow photobioreactor system according to claim 4, wherein said pigs are built in each of the cylindrical transparent pipes: a cylinder filled with foam, a gasket, and two rubber seals. Circle, two disc rubber gaskets with notches and two perforated discs.
The circulatory flow type photobioreactor system according to claim 5, wherein each of the cylindrical transparent pipes is internally provided with a pig, wherein the foam-filled cylinder is sequentially connected with a rubber seal ring and a notch. Disc rubber gaskets, gaskets and discs with perforations are formed by screw and nut connections.
A cylindrical pipe is built in each of the cylindrical transparent pipes of the circulating flow type photobioreactor system according to claim 5 or 6, wherein the disk rubber gasket having a notch has a plurality of allowable water The gap between the flow and the gap between the upper and lower gaps through which the upper and lower rubber gaskets pass is 45 degrees. The pig is rotated under the action of the water flow force of the water to ensure the seamless cleaning of the inner wall of the transparent pipe. All of its area.
The circulating flow photobioreactor system according to claim 1, wherein said photobioreactor unit: the lower portion and the upper portion are supported by the steel plate and the plurality of circulating transparent pipes and are buckled by the I-beam. It is fixed and 90° to the ground. There is a rubber ring between the connecting cylindrical transparent pipe and the pipe tee to prevent leakage, which is fixed by the buckle.
The circulating flow photobioreactor system according to claim 8, wherein said photobioreactor comprises: 20 to 500,000 cylindrical transparent pipes and pipe tees, I-beams, connecting members, and support frames. And the fixed photo-bioreactor unit is arranged in series, parallel, two rows of rows, connected in series, and the end-to-end connection extends to form a circulating flow photon The reactor has a height of 0.80 m to 6.00 m and a diameter of 0.05 m to 0.55 m.
The circulating flow type photobioreactor system according to claim 9, wherein the plurality of photobioreactor units are arranged in series, in parallel, in two rows, and connected in series, and the end-to-end connection and the extension are connected to form a circulating flow type. Photobioreactor; a plurality of circulating flow photobioreactors, which are then connected in parallel to form a circulating flow photobioreactor system.
A circulating flow photobioreactor system according to any of claims 1-10, characterized in that the microalgae growth liquid is dispensed in the mixing tank, and the flow velocity is 10-20 cm/sec. In the reactor system, the microalgae growth solution stays in each cylindrical transparent pipe, uniformly passes carbon dioxide, carries out photosynthesis, and the residence time of the microalgae growth solution is about 8-16 hours according to the microalgae seed. cycle. When the detector detects that the microalgae liquid is insufficiently nutritious, the microalgae liquid is returned to the culture solution by the inlet and outlet to prepare the storage tank, and the nutrient required for the growth of the appropriate amount of microalgae is added; and the next microalgae photobioreaction cycle is entered.
The circulating flow photobioreactor system according to claim 11, wherein the microalgae growth liquid is driven into the photobioreactor system by a liquid running pump to fully absorb the photosynthesis microalgae organism; in the photobioreactor The front end of the system device is equipped with a carbon dioxide gas storage tank, a distribution storage tank, an intermediate storage tank, and a harvest storage tank. For the first time, the microalgae seeds and the nutrient solution for plant growth are pumped into the photobioreactor system in the mixing tank, and then the opening is started. The carbon dioxide control valve into the cylindrical transparent pipe always supplements the carbon dioxide, keeps the carbon dioxide in the pH setting range of the nutrient solution, and keeps the microalgae plant in the optimal growth state for photosynthesis; the microalgae plant growth liquid according to the detection index Returning to the nutrition of the microalgae plant in the blending tank in time, when the microalgae reaches the set density, the microalgae growth liquid enters the harvesting tank, and the collector automatically collects the microalgae culture product.
A circulating flow photobioreactor system according to claim 12 wherein The microalgae growth liquid enters the circulating flow photobioreactor system, and the microalgae growth liquid stays in each of the cylindrical transparent pipes, and the inlet end of the inlet of the pipe tee is connected to the intake manifold of the automatic control valve, and is opened. The carbon dioxide control valve that passes into the cylindrical transparent pipe always supplements the carbon dioxide. When the pH of the slurry is higher or lower than the set range, the gas control valve adjusts to increase or decrease the amount of carbon dioxide replenishment within the pH setting range. Maintaining the most appropriate solubility of carbon dioxide, allowing microalgae plants to maintain optimal growth for photosynthesis.
A circulating flow photobioreactor system according to claim 13 wherein the intermediate storage tank, the mixing tank and the cylindrical transparent conduit are measured using a series of probes on the flue and pH and dissolved oxygen probe 15. The density of liquid microalgae, the carbon dioxide content in the gas phase is tracked by the carbon dioxide sensor to ensure that the system has enough carbon dioxide to guide the growth of algae, photosynthetically active radiation can be quantified using photosensors, measuring photon flux in the photosynthetic flux, dissolving The oxygen sensor is the product of the photosynthetic reaction used to track, ensuring that no excess oxygen is dissolved in the system.
The circulating flow photobioreactor system according to claim 1, wherein the algae is harvested when the set density is reached, and the microalgae liquid enters the harvesting tank from the inlet and outlet, from which algae can be concentrated by standard means, such as Flocculation, sedimentation, centrifugation, flotation, etc. are collected by a collector to obtain a microalgae culture product.