CN109337820A - The method of continuous production microalgae - Google Patents

The method of continuous production microalgae Download PDF

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Publication number
CN109337820A
CN109337820A CN201811502954.7A CN201811502954A CN109337820A CN 109337820 A CN109337820 A CN 109337820A CN 201811502954 A CN201811502954 A CN 201811502954A CN 109337820 A CN109337820 A CN 109337820A
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China
Prior art keywords
microalgae
optical
biological reaction
nitric acid
reaction tower
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Inventor
洪蕾
白玫
邓国华
杨凌夕
何小娟
吕世军
何琨
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China Petroleum and Chemical Corp
Sinopec Shanghai Engineering Co Ltd
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China Petroleum and Chemical Corp
Sinopec Shanghai Engineering Co Ltd
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Priority to CN201811502954.7A priority Critical patent/CN109337820A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • C02F2101/163Nitrates

Abstract

The present invention relates to a kind of method of continuous production microalgae, mainly solve the problems, such as that nitric acid waste reuse ratio is low in the prior art, microalgae production low efficiency.The present invention by using a kind of continuous production microalgae method, nitric acid waste is handled while cultivating microalgae using optical-biological reaction tower again, it refilters-centrifugation-and dries obtained microalgae product, nitric acid waste also realizes qualified discharge and " zero-emission ", two sets of optical-biological reaction systems mutually switched are set simultaneously, so that the technical solution for cultivating the progress of microalgae industry process continuous is preferably solved the above problem, can be used in continuous production microalgae.

Description

The method of continuous production microalgae
Technical field
The present invention relates to a kind of methods of continuous production microalgae, by environmental protection treatment nitric acid waste and cultivating microalgae product Combine, and two sets of optical-biological reaction systems mutually switched are set in process flow, makes the industrial process for cultivating microalgae Can serialization carry out, can be used for handling in nitric acid waste.
Background technique
The nitric acid waste containing nitric acid and nitrate ion that petrochemical process unit generates in process of production, mostly Environmental protection treatment is carried out using vaporizing extract process, membrane processing method, ion-exchange and the methods of evaporative condenser and neutralization.But these Either physically or chemically all existing cannot achieve the purpose further cut down with cycling and reutilization nitric acid waste.
Microalgae is a kind of very original living resources, and volume is small, structure is simple, widely distributed, without Organ Differentiation, And microalgae has that photosynthetic efficiency is high, the speed of growth is fast, biological yield is big, be easy to breed improvement, environmental suitability by force Feature, and compared with higher plant, the content of the effective components such as their lipid, starch and protein is higher.The biography of microalgae System commercial application field is very extensive, including uses them as food additives, products and health products additive agent, auxiliary pharmaceutical adjuvant and agriculture and animal husbandry The raw material of industry feed, culture fishery feed and chemical industry.
Number of patent application in the prior art is 200610102052.5 ammonium nitrate waste water treatment method of CN, discloses one Kind handles the processing method containing ammonium nitrate wastewater using multiple-effect evaporation method, the pressured neutral process suitable for the charging of high concentration nitric acid The processing of spent process water is also applied for other similar techniques, such as: sulfate, phosphate, nitrate, nitrite are non-volatile The wastewater treatment of property component.Number of patent application is that photosynthesis physiological target is used in a kind of production of the microalgae industry metaplasia of CN 03128138.9 System discloses a kind of using closed conduit microalgae industry metaplasia production photosynthetic organism reactor assembly, double reaction tower streams Journey realizes the continuous operation of both culturing microalgae;The invention cultivates microalgae product and uses carbon dioxide raw material, further relates to wastewater treatment The industrialization process combined with both culturing microalgae.Number of patent application is 200810201116.6 microdisk electrode coupled biological bavin of CN The production method of oil refining, discloses and a kind of biological diesel oil refining is coupled industrialized method with microdisk electrode;This method benefit It is primary raw material cultivation oil-containing micro-algae with carbon dioxide commercial exhaust gas and eutrophication industrial wastewater, while realizes carbon dioxide The big target of the three of the clean manufacturing of emission reduction, the improvement of polluted-water and biodiesel.
Number of patent application in the prior art is CN 200610102052.5 only with physical method processing petrochemical equipment The nitric acid wastes such as ammonium nitrate, the high concentration nitric acid of generation are not directed to handle nitric acid waste using biochemical method, thus nitric acid That there are construction investments during waste liquid environmental protection treatment is high, operating cost is high, maintenance is costly, nitric acid waste reuse ratio is low asks Topic.
Number of patent application is CN 03128138.9 and number of patent application is CN 200810201116.6 all using titanium dioxide Carbon carries out cultivation and cultivating microalgae product as raw material, but is not directed to the environmental protection treatment containing nitric acid waste or nitrate anion salt waste water With the technical solution of comprehensive utilization, existing environmental protection treatment nitric acid waste to combine with cultivating microalgae product simultaneously, is micro- The problem of algae production low efficiency.
In recent years, with the continuous development of Measurement for Biochemistry, environmental protection treatment nitric acid waste and cultivating microalgae are produced The technical solution that product combine, can not only reduce the toxigenic capacity of microalgae product, but also nitric acid waste pollutant may be implemented The requirement of nitric acid waste qualified discharge and " zero-emission " is realized in emission reduction.The side of nitric acid waste continuous production microalgae will be utilized as a result, Method will generate bigger economic benefit and environmental benefit for handling in nitric acid waste.
Summary of the invention
Low, microalgae production effect that the technical problem to be solved by the present invention is to nitric acid waste reuse ratios in the prior art The low problem of rate provides a kind of method of new continuous production microalgae, has nitric acid waste reuse ratio height, microalgae production High-efficient advantage.
To solve the above problems, The technical solution adopted by the invention is as follows: a kind of method of continuous production microalgae, including such as Lower step:
A) it collects in nitric acid waste storage tank (8), passes through from petrochemical process unit nitric acid waste to be processed (1) Dilution storage tank (10) is diluted processing, after neutralization storage tank (11) is neutralized, out-of-bounds contains glucose, phosphate with coming from And the nutritive solution (2) of zinc, cu microelements merges, then sub with coming from out-of-bounds and collecting the algae in algae storage tank (9) Liquid (3) merges, and three strands of materials and two strands of return materials merge into seed mixture liquid (4) into optical-biological reaction system;
B) 2 optical-biological reaction tower A (16) in parallel and optical-biological reaction tower B (17) is arranged in optical-biological reaction system, in which: The first control valve (12) and third control valve door (14), optical-biological reaction tower B is arranged in optical-biological reaction tower A (16) upstream and downstream (17) upstream and downstream setting the second control valve (13) and the 4th control valve (15);When the first control valve (12) and third control When valve (14) is opened, optical-biological reaction tower A (16) is operating condition, nitric acid waste (1) and nutritive solution (2) and algae Liquid (3) three strands of materials and two strands of return materials merge into seed mixture liquid (4) into optical-biological reaction tower A (16), in LED illumination Under the conditions of air aeration, photosynthesis occurs for microalgae seed, can adapt to microalgae in the environment of high concentration nitric acid waste The nitrate nitrogen in nitric acid waste is quickly absorbed, microalgae being capable of rapid fermented and cultured;Also microalgae is enable to absorb residual in nitric acid waste Remaining nitrate nitrogen be properly completed nitric acid waste processing can, and microalgae can further react growth;Second control valve at this time (13) it is closed with the 4th control valve (15), optical-biological reaction tower B (17) is disinfection operating condition, and high-temperature steam is passed through optical-biological reaction Tower B (17) carries out disinfection sterilization operation;It is switched by control valve, switches optical-biological reaction tower A (16) and optical-biological reaction tower B (17) operation operating condition, to guarantee being carried out continuously for both culturing microalgae cultivation;
C) the microalgae solution for completing cultivating step is sequentially sent to filter (18) and centrifuge (19), through filter (18) cleaner liquid (5) excessively that generate and the centrifugal clear liquid (6) generated through centrifuge (19) merge through centrifugal clear liquid storage tank (20), and two Stock returns to material and is fed again into the cultivating that optical-biological reaction system participates in microalgae;Through filter (18) and centrifuge (19) Microalgae solution be sent into drier (21) it is dry, finally obtain microalgae powder-product (7).
In above-mentioned technical proposal, it is preferable that nitric acid waste treating capacity to be processed is 0.1~2000L/ period, with HNO3 Meter, concentration of nitric acid are 0.1~100g/L.
In above-mentioned technical proposal, it is preferable that optical-biological reaction tower A (16) and optical-biological reaction tower B (17) is set equipped with aeration Standby and LED light source device, to guarantee the photosynthesis of microalgae;Aerator input air flow is 1~20000Nm3/ hr, LED Light source equipment illumination is 50~450lx.
In above-mentioned technical proposal, it is preferable that optical-biological reaction tower A (16) and optical-biological reaction tower B (17) is steamed equipped with high temperature Steam pipe line, to guarantee that disinfection and sterilization operates;High temperature steam temperature be 110~200 DEG C, moise-heat sterilization sterilizing time be 15~ 45min。
In above-mentioned technical proposal, it is preferable that optical-biological reaction tower, in operating condition, operating pressure be 0.12~ 0.26MPaA, operation temperature are 20~40 DEG C, and temperature-constant operation, temperature fluctuation range is ± 0.5 DEG C, pH=6.5~10.0, stirring Revolving speed is 50~450rpm, and the microdisk electrode period is 12~144h, and the cycle of operation is 2~18 days.
In above-mentioned technical proposal, it is preferable that filter (18) entry operation pressure is 0.40~0.60MPaA, export operation Pressure be 0.35~0.55MPaA, operation temperature be 20~40 DEG C, filter membrane component retain microalgae relative molecular weight be 20000~ 500000, filtered dense algae solution dry matter solid content is 10~50g/L.
In above-mentioned technical proposal, it is preferable that centrifuge (19) entry operation pressure is 0.30~0.50MPaA, export operation Pressure is 0.25~0.45MPaA, and operation temperature is 20~40 DEG C, dense algae solution dry matter solid content after centrifugation is 100~ 350g/L。
In above-mentioned technical proposal, it is preferable that the operating pressure of drier be 0.10~0.40MPaA, operation temperature be 20~ 40 DEG C, microalgae powder product yield is 0.07~1408kg/d.
The present invention relates to a kind of method using nitric acid waste continuous production microalgae, seek to solve nitric acid waste recycling The problem of rate is low, microalgae production low efficiency.The nitric acid waste environmental protection treatment that the present invention generates petrochemical process units with it is micro- The cultivation of algae product cultivating combines, and microalgae product cultivating is cultivated and is amplified to continuous industrialization by the laboratory scale of interval Comercial operation scale.Two optical-biological reaction towers are set simultaneously, are switched by control valve, an optical-biological reaction tower is carried out The operation of environmental protection treatment nitric acid waste and cultivating microalgae product, another optical-biological reaction tower carry out disinfection sterilization operation, To realize the industrialization comercial operation for utilizing nitric acid waste continuous production microalgae product.Nitric acid waste is utilized the present invention relates to a kind of The method of continuous production microalgae carries out nitric acid waste while carrying out both culturing microalgae cultivation using optical-biological reaction tower first again Then environmental protection treatment recycles and is separated by filtration-is centrifugated-obtained microalgae powder-product of powder drying and other steps, nitric acid waste Also achieve qualified discharge and " zero-emission ".Meanwhile two sets of optical-biological reaction systems that can mutually switch are set, make to cultivate micro- The industrial process serialization of algae carries out.Thus with HNO3Meter, the present invention are capable of handling the nitric acid that concentration of nitric acid is 0.1~100g/L Waste liquid, and obtain yield be 0.07~1408kg/d high added value microalgae powder-product, achieve preferable technical effect and Environmental benefit.
Detailed description of the invention
Fig. 1 is the flow diagram of the method for the invention.
In Fig. 1,1, nitric acid waste;2, nutritive solution;3, algae seed liquor;4, seed mixture liquid;5, cleaner liquid is crossed;6, it is centrifuged Clear liquid;7, microalgae powder-product;8, nitric acid waste storage tank;9, algae storage tank;10, storage tank is diluted;11, storage tank is neutralized;12, first Control valve;13, the second control valve;14, third control valve door;15, the 4th control valve;16, optical-biological reaction tower A;17, Optical-biological reaction tower B;18, filter;19, centrifuge;20, centrifugal clear liquid storage tank;21, drier.
The present invention will be further described below by way of examples, but is not limited only to the present embodiment.
Specific embodiment
[comparative example 1]
The prior art 12 days tower A cycles of operation of optical-biological reaction stop sterilizing 4 days;Optical-biological reaction tower B operation week It phase 12 days, stops sterilizing 4 days;Microalgae powder product yield is 264kg/d.
[embodiment 1]
The method that nitric acid waste continuous production microalgae is utilized using the present invention, as shown in Figure 1, with optical-biological reaction tower A (16) operating condition and optical-biological reaction tower B (17) disinfection operating condition for: the nitric acid waste (1) from petrochemical process unit It collects in nitric acid waste storage tank (8), is diluted processing by diluting storage tank (10), neutralizes storage tank (11) and be neutralized Afterwards, merge with from the nutritive solution (2) out-of-bounds containing microelements such as glucose, phosphate, zinc, copper, then with from out-of-bounds simultaneously The algae seed liquor (3) collected in algae storage tank (9) merges, and three strands of materials and two strands of return materials merge into seed mixture liquid (4) enter optical-biological reaction system.Optical-biological reaction system setting two optical-biological reaction tower A (16) and photo-biological in parallel are anti- Tower B (17) are answered, when the first control valve (12) and third control valve door (14) opening, optical-biological reaction tower A (16) is operation Operating condition, nitric acid waste (1) and nutritive solution (2) and algae seed liquor (3) three strands of materials and two strands of return materials merge into mixing Seed liquor (4) enters optical-biological reaction tower A (16), and under the conditions of LED illumination and air aeration, photosynthetic work occurs for microalgae seed With making microalgae can adapt to quickly absorb the nitrate nitrogen in nitric acid waste in the environment of high concentration nitric acid waste, microalgae can Rapid fermented and cultured;Also so that microalgae is absorbed nitrate nitrogen remaining in nitric acid waste, keep nitric acid waste processing thoroughly complete At microalgae can further react growth.The second control valve (13) and the 4th control valve (15) are closed at this time, and photo-biological is anti- Answer tower B (17) for disinfection operating condition, high-temperature steam is passed through optical-biological reaction tower B (17) and carries out disinfection sterilization operation.Pass through control valve Door switch can switch the operation operating condition of optical-biological reaction tower A (16) and optical-biological reaction tower B (17), to guarantee both culturing microalgae That cultivates is carried out continuously.C) the microalgae solution for completing cultivating step is sequentially sent to filter (18) and centrifuge (19), Cleaner liquid (5) and the centrifugal clear liquid (6) generated through centrifuge (19) are crossed through centrifugal clear liquid storage tank through what filter (18) generated (20) merge, two strands return to material and are fed again into the cultivating that optical-biological reaction system participates in microalgae.Through filter (18) It is sent into drier (21) drying with the microalgae solution of centrifuge (19), finally obtains microalgae powder-product (7).
The nitric acid waste treating capacity 500L/ period to be processed, with HNO3Meter, concentration of nitric acid 68g/L.In operating condition When, optical-biological reaction tower A (16) aerator input air flow is 5000Nm3/ hr, LED light source device illumination are 110lx, Operating pressure is 0.20MPaA, and operation temperature is 33 DEG C, and temperature-constant operation, temperature fluctuation range is ± 0.5 DEG C, pH=8.6, stirring Revolving speed is 130rpm, and the microdisk electrode period is 42h, and the cycle of operation is 12 days.When sterilizing operating condition, optical-biological reaction tower B (17) High temperature steam temperature is 170 DEG C, and moise-heat sterilization sterilizing time is 38min.Filter (18) entry operation pressure is 0.52MPaA, Export operation pressure is 0.47MPaA, and operation temperature is 33 DEG C, and the relative molecular weight that filter membrane component retains microalgae is 82000, mistake Dense algae solution dry matter solid content after filter is 42g/L.Centrifuge (19) entry operation pressure is 0.42MPaA, export operation pressure For 0.37MPaA, operation temperature is 33 DEG C, and the dense algae solution dry matter solid content after centrifugation is 215g/L.The operating pressure of drier For 0.32MPaA, operation temperature is 33 DEG C, and microalgae powder product yield is 352kg/d.It is micro- compared with [comparative example 1] 264kg/d Algae powder-product yield increases by 33.3%.
[comparative example 2]
The prior art 12 days tower A cycles of operation of optical-biological reaction stop sterilizing 4 days;Optical-biological reaction tower B operation week It phase 12 days, stops sterilizing 4 days;Microalgae powder product yield is 0.05kg/d.
[embodiment 2]
Process condition is with [embodiment 1], and only nitric acid waste treating capacity to be processed changes.It is utilized using the present invention The method of nitric acid waste continuous production microalgae, nitric acid waste treating capacity 0.1L/ period to be processed, with HNO3Meter, concentration of nitric acid For 68g/L.In operating condition, optical-biological reaction tower A (16) aerator input air flow is 1Nm3/ hr, LED light source are set Standby illumination is 110lx, and operating pressure 0.20MPaA, operation temperature is 33 DEG C, and temperature-constant operation, temperature fluctuation range is ± 0.5 DEG C, pH=8.6, speed of agitator 130rpm, the microdisk electrode period are 42h, and the cycle of operation is 12 days.When sterilizing operating condition, light Biological respinse tower B (17) high temperature steam temperature is 170 DEG C, and moise-heat sterilization sterilizing time is 38min.Filter (18) entry operation Pressure is 0.52MPaA, and export operation pressure is 0.47MPaA, and operation temperature is 33 DEG C, and filter membrane component retains opposite point of microalgae Son amount is 82000, and filtered dense algae solution dry matter solid content is 42g/L.Centrifuge (19) entry operation pressure is 0.42MPaA, export operation pressure are 0.37MPaA, and operation temperature is 33 DEG C, and the dense algae solution dry matter solid content after centrifugation is 215g/L.The operating pressure of drier is 0.32MPaA, and operation temperature is 33 DEG C, and microalgae powder product yield is 0.07kg/d. Compared with [comparative example 2] 0.05kg/d, microalgae powder product yield increases by 40.0%.
[comparative example 3]
The prior art 12 days tower A cycles of operation of optical-biological reaction stop sterilizing 4 days;Optical-biological reaction tower B operation week It phase 12 days, stops sterilizing 4 days;Microalgae powder product yield is 1056kg/d.
[embodiment 3]
Process condition is with [embodiment 1], and only nitric acid waste treating capacity to be processed changes.It is utilized using the present invention The method of nitric acid waste continuous production microalgae, nitric acid waste treating capacity 2000L/ period to be processed, with HNO3Meter, concentration of nitric acid For 68g/L.In operating condition, optical-biological reaction tower A (16) aerator input air flow is 20000Nm3/ hr, LED light Source device illumination be 110lx, operating pressure 0.20MPaA, operation temperature be 33 DEG C, temperature-constant operation, temperature fluctuation range be ± 0.5 DEG C, pH=8.6, speed of agitator 130rpm, the microdisk electrode period is 42h, and the cycle of operation is 12 days.When sterilizing operating condition, Optical-biological reaction tower B (17) high temperature steam temperature is 170 DEG C, and moise-heat sterilization sterilizing time is 38min.Filter (18) entrance behaviour Making pressure is 0.52MPaA, and export operation pressure is 0.47MPaA, and operation temperature is 33 DEG C, and filter membrane component retains the opposite of microalgae Molecular weight is 82000, and filtered dense algae solution dry matter solid content is 42g/L.Centrifuge (19) entry operation pressure is 0.42MPaA, export operation pressure are 0.37MPaA, and operation temperature is 33 DEG C, and the dense algae solution dry matter solid content after centrifugation is 215g/L.The operating pressure of drier is 0.32MPaA, and operation temperature is 33 DEG C, and microalgae powder product yield is 1408kg/d. Compared with [comparative example 3] 1056kg/d, microalgae powder product yield increases by 33.3%.
[comparative example 4]
The prior art 2 days tower A cycles of operation of optical-biological reaction stop sterilizing 1 day;Optical-biological reaction tower B operation week It phase 2 days, stops sterilizing 1 day;Microalgae powder product yield is 141kg/d.
[embodiment 4]
Nitric acid waste treating capacity to be processed is with [embodiment 1], and only process condition changes.It is utilized using the present invention The method of nitric acid waste continuous production microalgae, nitric acid waste treating capacity 500L/ period to be processed, with HNO3Meter, concentration of nitric acid For 0.1g/L.In operating condition, optical-biological reaction tower A (16) aerator input air flow is 5000Nm3/ hr, LED light Source device illumination be 50lx, operating pressure 0.12MPaA, operation temperature be 20 DEG C, temperature-constant operation, temperature fluctuation range be ± 0.5 DEG C, pH=6.5, speed of agitator 50rpm, the microdisk electrode period is 12h, and the cycle of operation is 2 days.When sterilizing operating condition, light Biological respinse tower B (17) high temperature steam temperature is 110 DEG C, and moise-heat sterilization sterilizing time is 45min.Filter (18) entry operation Pressure is 0.40MPaA, and export operation pressure is 0.35MPaA, and operation temperature is 20 DEG C, and filter membrane component retains opposite point of microalgae Son amount is 20000, and filtered dense algae solution dry matter solid content is 10g/L.Centrifuge (19) entry operation pressure is 0.30MPaA, export operation pressure are 0.25MPaA, and operation temperature is 20 DEG C, and the dense algae solution dry matter solid content after centrifugation is 100g/L.The operating pressure of drier is 0.10MPaA, and operation temperature is 20 DEG C, and microalgae powder product yield is 212kg/d.With [comparative example 4] 141kg/d is compared, and microalgae powder product yield increases by 50.4%.
[comparative example 5]
The prior art 18 days tower A cycles of operation of optical-biological reaction stop sterilizing 5 days;Optical-biological reaction tower B operation week It phase 18 days, stops sterilizing 5 days;Microalgae powder product yield is 191kg/d.
[embodiment 5]
Nitric acid waste treating capacity to be processed is with [embodiment 1], and only process condition changes.It is utilized using the present invention The method of nitric acid waste continuous production microalgae, nitric acid waste treating capacity 500L/ period to be processed, with HNO3Meter, concentration of nitric acid For 100g/L.In operating condition, optical-biological reaction tower A (16) aerator input air flow is 5000Nm3/ hr, LED light Source device illumination be 450lx, operating pressure 0.26MPaA, operation temperature be 40 DEG C, temperature-constant operation, temperature fluctuation range be ± 0.5 DEG C, pH=10.0, speed of agitator 450rpm, the microdisk electrode period is 144h, and the cycle of operation is 18 days.In disinfection operating condition When, optical-biological reaction tower B (17) high temperature steam temperature is 200 DEG C, and moise-heat sterilization sterilizing time is 15min.Filter (18) enters Mouth operating pressure is 0.60MPaA, and export operation pressure is 0.55MPaA, and operation temperature is 40 DEG C, and filter membrane component retains microalgae Relative molecular weight is 500000, and filtered dense algae solution dry matter solid content is 50g/L.Centrifuge (19) entry operation pressure is 0.50MPaA, export operation pressure are 0.45MPaA, and operation temperature is 40 DEG C, and the dense algae solution dry matter solid content after centrifugation is 350g/L.The operating pressure of drier is 0.40MPaA, and operation temperature is 40 DEG C, and microalgae powder product yield is 244kg/d.With [comparative example 5] 191kg/d is compared, and microalgae powder product yield increases by 27.7%.

Claims (8)

1. a kind of method of continuous production microalgae, includes the following steps:
A) nitric acid waste from petrochemical process unit (1) is collected in nitric acid waste storage tank (8), by diluting storage tank (10) be diluted processing, neutralize after storage tank (11) be neutralized, with from out-of-bounds containing glucose, phosphate and zinc, The nutritive solution (2) of cu microelements merges, then closes with coming from out-of-bounds and collecting the algae seed liquor (3) in algae storage tank (9) And three strands of materials and two strands of return materials merge into seed mixture liquid (4) into optical-biological reaction system;
B) 2 optical-biological reaction tower A (16) in parallel and optical-biological reaction tower B (17) is arranged in optical-biological reaction system, in which: photoproduction Object reaction tower A (16) upstream and downstream is arranged the first control valve (12) and third control valve door (14), on optical-biological reaction tower B (17) The second control valve (13) and the 4th control valve (15) is arranged in downstream;When the first control valve (12) and third control valve door (14) when opening, optical-biological reaction tower A (16) is operating condition, nitric acid waste (1) and nutritive solution (2) and algae seed liquor (3) three strands of materials and two strands of return materials merge into seed mixture liquid (4) into optical-biological reaction tower A (16), in LED illumination and Under the conditions of air aeration, photosynthesis occurs for microalgae seed, can adapt to microalgae fast in the environment of high concentration nitric acid waste Speed absorbs the nitrate nitrogen in nitric acid waste, and microalgae being capable of rapid fermented and cultured;Also microalgae is enable to absorb remaining in nitric acid waste Nitrate nitrogen, make nitric acid waste processing can be properly completed, microalgae can further react growth;Second control valve at this time (13) it is closed with the 4th control valve (15), optical-biological reaction tower B (17) is disinfection operating condition, and high-temperature steam is passed through optical-biological reaction Tower B (17) carries out disinfection sterilization operation;It is switched by control valve, switches optical-biological reaction tower A (16) and optical-biological reaction tower B (17) operation operating condition, to guarantee being carried out continuously for both culturing microalgae cultivation;
C) the microalgae solution for completing cultivating step is sequentially sent to filter (18) and centrifuge (19), through filter (18) Generation crosses cleaner liquid (5) and the centrifugal clear liquid (6) generated through centrifuge (19) through centrifugal clear liquid storage tank (20) merging, and two strands are returned It returns material and is fed again into the cultivating that optical-biological reaction system participates in microalgae;It is micro- through filter (18) and centrifuge (19) Algae solution is sent into drier (21) drying, finally obtains microalgae powder-product (7).
2. the method for continuous production microalgae according to claim 1, it is characterised in that nitric acid waste treating capacity to be processed is 0.1~2000L/ period, with HNO3Meter, concentration of nitric acid are 0.1~100g/L.
3. the method for continuous production microalgae according to claim 1, it is characterised in that optical-biological reaction tower A (16) and photo-biological Reaction tower B (17) is furnished with aerator and LED light source device, to guarantee the photosynthesis of microalgae;Aerator input air stream Amount is 1~20000Nm3/ hr, LED light source device illumination are 50~450lx.
4. the method for continuous production microalgae according to claim 1, it is characterised in that optical-biological reaction tower A (16) and photo-biological Reaction tower B (17) is furnished with high-temperature steam pipeline, to guarantee that disinfection and sterilization operates;High temperature steam temperature is 110~200 DEG C, steam The disinfection and sterilization time is 15~45min.
5. the method for continuous production microalgae according to claim 1, it is characterised in that optical-biological reaction tower, in operating condition When, operating pressure is 0.12~0.26MPaA, and operation temperature is 20~40 DEG C, and temperature-constant operation, temperature fluctuation range is ± 0.5 DEG C, pH=6.5~10.0, speed of agitator is 50~450rpm, and the microdisk electrode period is 12~144h, and the cycle of operation is 2~18 It.
6. the method for continuous production microalgae according to claim 1, it is characterised in that filter (18) entry operation pressure is 0.40~0.60MPaA, export operation pressure are 0.35~0.55MPaA, and operation temperature is 20~40 DEG C, and the retention of filter membrane component is micro- The relative molecular weight of algae is 20000~500000, and filtered dense algae solution dry matter solid content is 10~50g/L.
7. the method for continuous production microalgae according to claim 1, it is characterised in that centrifuge (19) entry operation pressure is 0.30~0.50MPaA, export operation pressure are 0.25~0.45MPaA, and operation temperature is 20~40 DEG C, the dense algae solution after centrifugation Dry matter solid content is 100~350g/L.
8. the method for continuous production microalgae according to claim 1, it is characterised in that the operating pressure of drier be 0.10~ 0.40MPaA, operation temperature are 20~40 DEG C, and microalgae powder product yield is 0.07~1408kg/d.
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