CN105039134B - Recycle stream dynamic formula photo-bioreactor system - Google Patents
Recycle stream dynamic formula photo-bioreactor system Download PDFInfo
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Abstract
The invention discloses a kind of recycle stream dynamic formula photo-bioreactor system, hold-up vessel 1, liquid operation pump 2, recovery/drainage recurrence pipeline 3, recovery/recurrence pipe 4, spilling/liquid, gas return pipe 5, bioreactor 6, harvest mouth 7, harvest storage tank 8, waste liq/useless nutrient solution 9, ultraviolet sterilization apparatus 10, the biomass product 11 concentrated, recurrent canal 12, flue gas/carbon dioxide imported unit 13, gas operation pump 14, flue and pH value and dissolved oxygen probe 15.The recycle stream dynamic formula photo-bioreactor system of the present invention overcome product in the prior art lack temperature, illumination, mix that uneven, volume is small, it is easy microbiological contamination, difficult in maintenance, the other bioreactor produced problems of almost all such as difficulty are extracted, automation closed culture is realized.
Description
Technical field
It is many by photosynthesis culture in particular to one kind the present invention relates to a kind of closed photo bioreactor system
Photosynthetic microalgae biology is planted, the recycle stream dynamic formula photo-bioreactor system of industrialization both culturing microalgae is carried out.
Background technology
Photosynthetic microalgae is biological, especially rich oil energy microalgae, plastics microalgae, haematococcus pluvialis, green alga, blue-green algae, ball algae etc.
The growth and breeding of (abbreviation algae) relies primarily on corresponding mineral matter.Single celled algae has extremely strong fertility, in light
Substantial amounts of carbon dioxide can be absorbed under cooperation.Algae utilizes luminous energy, water, mineral matter and carbon dioxide by photosynthesis
Deng cycling of elements into rich in a large amount of greases, protein, astaxanthin, anti-oxidant contour attached value organic compound.
Different alga microbials are cultivated by bioreactor, a variety of different high value added products can be harvested.
And because algae has the quick nutriment such as absorbing carbon dioxide, nitrogen, phosphorus from surrounding environment and is translated into organic
The ability of compound (being such as stored in grease, albumen, the astaxanthin in cell), it can be also used for purification waste water and recycling
Thermal power plant rich carbonated flue gas improves environment.Algae can play important during biological cleaning in systems
Part, after maturation, by system process, output a large amount of bio-fuels (such as biodiesel, alcohol and first such as collection, extractions
Alkane etc.), its frond also can as animal feed and organic fertilizer, and improved soil important source material.In algae aquaculture
In, its economic benefit produced is by the selection of algae, the scale of cultivation, the application efficiency of bioreactor design and application
Into what is determined originally.
The bioreactor that there is scale the current country is generally open pond, transverse tube, pipeline, transparent light film, plastic foil etc.,
Building up 1000~5000 square metres of annular shallow ponds (15~30 centimetres of the depth of water) more in open the pond, nutrient solution is followed with manpower or paddle wheel
Circulation is moved.This production model has significant limitation, and its is simple in construction, although has the advantages that investment is low and easily builds, but together
The efficiency of sample is excessively low, and algae grows out of doors many uncontrollable factors, and such as opening is pool cultivated to be lacked temperature, illumination, mixes
Close uneven, volume small, be easy to microbiological contamination with air contact so that overall productivity is low, often result in culture failure.
Open defect has promoted the development of closed culture systems, with transparent pipeline, flat or other containers
The closed photo bioreactor being made is occurred in that.Transverse tube type, flat-plate reactor application it is also low by its high cost, efficiency
Limitation, be only used for production small lot, high added value Special Products；The bioreactor of other species also because of many reasons such as
Extract that difficult, construction cost is high, volume is small, cleaning difficulty etc. and in succession can not large-scale farming produce economic benefit, limit
The development scale of microalgae industry.
Patent document (publication number WO 2008/151376A1) discloses a kind of film reactor, still, film reaction
There is also following shortcoming for device：
A, the construction unit size of reactor can not be adjusted after determining；
B, manufacture craft are complex, and each film unit side is in wavy, it is necessary to hot pick or other auxiliary processes；
C, because reactor width is smaller, while wavy sidewalls cause internal run-through poor, there is a dead angle, cleaning is very not
It is convenient；
D, due to the reactor side be main smooth surface, reactor pose pattern (direction) is restricted；
E, reactor are deeper, and the stress distribution on side plate is uneven, and material top mechanical property utilization rate is low.Meanwhile, hydraulic pressure
Material performance requirement to membrane wall is higher.Material cost is increased.
The content of the invention
An object of the present invention is to provide a kind of recycle stream dynamic formula photo-bioreactor system.
The second object of the present invention is to provide a kind of recycle stream dynamic formula bioreactor and recycle stream dynamic formula photo-biological is anti-
Answer device unit.
The third object of the present invention, which is to provide, a kind of can clean the wiper of transparent pipeline inwall automatically.
The these and other objects of the present invention will be expanded on further by the following detailed description and explanation.
The content of the invention
The recycle stream dynamic formula photo-bioreactor system of the present invention, including hold-up vessel 1, liquid run pump 2, reclaim/draw
Flow back to return pipeline 3, recovery/recurrence pipe 4, spilling/liquid, gas return pipe 5, bioreactor 6, harvest mouth 7, harvest storage tank 8,
Waste liq/useless nutrient solution 9, ultraviolet sterilization apparatus 10, the biomass product 11 of concentration, recurrent canal 12, flue gas/carbon dioxide
Imported unit 13, gas operation pump 14, flue and pH value and dissolved oxygen probe 15.Deployed nutrient solution is wherein put into centre
In storage tank 1, pump 2 is run by liquid and enters the lower section that nutrient input is sent to bioreactor 6, while being run by gas
Pump 14 is carried out the microalgae that flue gas/carbon dioxide is introduced in bioreactor after the completion of photosynthesis, photosynthesis, is supported
Grow microalgae density saturated solution and pipeline 3 is returned by recovery/drainage, recovery/recurrence is passed through in the case where liquid runs the dynamic action of pump 2
Pipe 4, enters in harvest storage tank 8 through harvesting mouth 7, the micro algae biomass product 11 of solid-liquor separation removal concentration, and remaining waste liq/
Useless nutrient solution 9 is returned to re-start in hold-up vessel 1 after the sterilization of UV-device 10 and used after nutrition deployment.From photo-biological
The gas and liquid that the top of reactor 6 is overflowed return pipe 5 by spilling/liquid, gas and also return to hold-up vessel 1 through recurrent canal 12
In re-start and used after nutrition deployment.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, described bioreactor 6 has side by side many
The individual transparent shape pipeline of transparent column is constituted, and the upper and lower part of cylindrical transparent pipeline connects insertion, every group of photoproduction by threeway
Thing reactor unit is made up of two bioreactors for being staggered at 45 ° 6, the top of two bioreactors 6 and
Bottom is interconnected to an inlet and outlet, and the transparent shape pipeline of described transparent column and connection threeway are by poly- terephthaldehyde
Sour glycol ester is made.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, connect in the bottom of described bioreactor 6
There is multiple be used in the state of setting certain pressure of being gathered on an air shooter line, described air shooter line in siphunculus
The aperture of gas effusion.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, in each circle of described bioreactor 6
It is built-in in the transparent shape pipeline of post that wiper is installed.Described each wiper includes:Lathery cylinder, pad,
Two rubber seal, two have disk rubber sheet gaskets jaggy and two and have porous disk；Wiper is foam
Cylinder is sequentially connected rubber seal, has disk rubber sheet gasket jaggy, pad and with porous disk up and down,
Pass through screw rod and nut connection shaping.Having has several breach for allowing enough current logical on disk rubber sheet gasket jaggy, on
Angle between the breach up and down that the reserved current of lower rubber sheet gasket pass through is 45 degree, and the breach of rubber sheet gasket is 4 up and down,
Wiper is rotated in the presence of the current turn of speed that water passes through, to ensure that transparent pipeline inside tube wall seamless cleans its all face
Product.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, the bottom of described bioreactor unit,
Top I-steel branch pipe tee connection and it is multiple circulate pipeline support and it is fixed by snap get off, it is and in 90 ° with ground,
There is the rubber ring for preventing leakage between connector between the transparent shape pipeline of cylinder and branch pipe tee connection, fixed by buckle.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, multiple bioreactor unit series connection, with two
It is behavior unit, arranged in parallel, overlook head and the tail connection extension and communicate, constitute recycle stream dynamic formula bioreactor, multiple circulations
Flow-type bioreactor is connected in parallel again constitutes recycle stream dynamic formula photo-bioreactor system.Described photo-biological is anti-
Answer device：By 20-500000 cylindrical transparent pipeline and branch pipe tee connection, I-steel, connector, support frame and fixation constitutes photoproduction
The series connection of thing reactor unit embarks on journey, two behavior units, arranged in parallel, is cascaded, overlooks head and the tail connection extension and communicate, constitute
Recycle stream dynamic formula bioreactor；Multiple recycle stream dynamic formula bioreactors are connected in parallel again constitutes recycle stream dynamic formula
Photo-bioreactor system.Cylindrical transparent pipeline is highly 0.80 meter -6.00 meters, a diameter of 0.05 meter -0.55 meter.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, after micro algae growth liquid is deployed in blend tank,
Using flowing velocity as 10-20 cels, into recycle stream dynamic formula photo-bioreactor system, micro algae growth liquid rests on each
Cylindrical transparent pipeline, is uniformly passed through carbon dioxide, carries out photosynthesis, micro algae growth liquid residence time, according to microalgae kind
The difference of son, about 8-16 hours is a cycle period.Detector is when detecting microalgae liquid nutritional deficiency, by importing and exporting
Nutriment microalgae liquid returned to required for the appropriate micro algae growth of nutrient solution allotment storage tank, supplement；Into next micro-
Algae optical biological reaction cycle.
Bioreactor is squeezed into by running pump by micro algae growth liquid, fully absorbs photosynthetic microalgae biology, in photoproduction
The front end of thing reactor assembly device is equipped with carbon dioxide air accumulator, allotment storage tank, harvest storage tank, first micro- in blend tank
Algae seed, the nutrient solution of plant growth are pumped into photo-bioreactor system together, are then turned on being passed through the titanium dioxide of cylindrical pipe
Carbon control valve, supplements carbon dioxide always, remains carbon dioxide and in pH value setting range, allows microalgae plant to protect in nutrient solution
Hold optimum growh state and carry out photosynthesis；Microalgae plant growth liquid is returned in blend tank in time according to Testing index and supplemented
The nutrition of microalgae plant growth, when microalgae liquid reaches setting density, microalgae liquid enters harvest storage tank, and by collector, (liquid is solid
Seperator) it can collect automatically, obtain both culturing microalgae product.
Micro algae growth liquid enters recycle stream dynamic formula photo-bioreactor system, and micro algae growth liquid rests on each cylinder thoroughly
In open conduit, the air admission hole upstream end of branch pipe tee connection bottom is connected to the inlet manifold of autocontrol valve, and it is saturating that unlatching is passed through cylinder
The carbon dioxide control valve of bright shape pipeline, supplements carbon dioxide always, when the pH value of slurry is higher or lower than setting range, gas
Control valve regulation increases or decreases the amount of carbon dioxide supplement, in pH value setting range, remains carbon dioxide most appropriate molten
Xie Du, allows microalgae plant to keep optimum growh state to carry out photosynthesis.
It is saturating to determine hold-up vessel, blend tank and cylinder with a series of probe on flue and pH value and dissolved oxygen probe 15
Liquid microalgae density in bright shape pipeline, carbon dioxide content in the gas phase is tracked by carbon dioxide sensor, to ensure
System has enough carbon dioxide to go to guide the growth of algae, and photosynthetically active radiation can use quantum sensor, measure photon
Quantify in photosynthetic spectrum flux, dissolved oxygen sensor is the product of the photosynthetic response for tracking, it is ensured that in systems without excessive
Oxygen dissolving.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, algae harvest is carried out when reaching setting density,
Microalgae liquid enters harvest storage tank by importing and exporting, and can collect algae by standard mode from here：Such as flocculation, precipitation, centrifugation
Separate, flotation etc. is collected by collector, obtain both culturing microalgae product.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, described recycle stream dynamic formula bioreactor bag
Include air-liquid bulk detector, sensor, operation liquid pump, air pump, hold-up vessel, liquid-solid separating centrifuge, bioreactor and component
(the transparent shape pipeline of cylinder and branch pipe tee connection, support frame, connector etc.), liquid transmission pipeline, carbon dioxide conveying circulation
Pipeline and carbon dioxide air accumulator, time liquid menifold, allotment storage tank, harvest storage tank, wherein：By transfer pipeline, operation pump by
Between storage tank, allotment storage tank and photo-bioreactor system connection, the cultivating microalgae high density come out from photo-bioreactor system
Liquid, enters harvest storage tank by time liquid menifold to harvest mouthful；The solid come out through flocculation, liquid-solid separating centrifuge is used for for microalgae product
Reprocessing, waste liq is given back by carrying out the addition such as seed, nutrient again into allotment storage tank after hold-up vessel and ultraviolet sterilization,
The amount into photo-bioreactor system is controlled by detector；The carbon dioxide ejected from photo-bioreactor system gives up
Gas and the gas and liquid that are overflowed from the top of bioreactor 6 are returned pipe 5 and returned through recurrent canal 12 by spilling/liquid, gas
Return in hold-up vessel 1 and use again after nutrition deployment；Circulation line is conveyed through carbon dioxide, is controlled by gas detector
System enters the amount of photo-bioreactor system carbon dioxide.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, described recycle stream dynamic formula bioreactor material
The selection of material and chain joint assembly：Thin-walled PET is managed, and is commonly used for the material of packaging, is constituted with the polyvinyl chloride pipe fitting of US spec 40
The main body of bioreactor, thinner transverse tube above is the blast pipe under system quiescence state；Here is cylindrical pipe
Branch pipe tee connection is circulated pipeline branch by threeway, the transparent shape pipeline of cylinder, the bottom of connector or top I-steel multiple
Support and be fixed up, the series connection of recycle stream dynamic formula bioreactor unit, parallel, two behavior units arrangement are overlooked head and the tail and connected
Extension is communicated.In order to ensure that sealing is water-tight, appropriately sized elastic tape is used as creating the pad between pipeline and accessory, makes
Sealing is reinforced with a worm-drive clip.Vertical pipe is that bottom I-steel is recycle stream by prefabricated I-shaped steel frame
The series connection of dynamic formula bioreactor unit is overlooked head and the tail connection extension and communicated, and supports and is fixed up, keeps the stability of system；
Top is also to be fixed by I-steel, without being suspended from top.Support frame is uniformly laid along parallel pipe deferent, support frame
Height is not less than the transparent shape pipeline of cylinder and the height of branch pipe tee connection；90 degree perpendicular to the ground of support frame, per the rectangular rectangle in side, directly
The rectangular vertical edges in angle are support frame column, overlook and are communicated to connect extension from beginning to end.Each recycle stream dynamic formula photo-biological
Reactor assembly all has the ability of certain scale and independent continuous culture microalgae, and the expense produced in independent operating is low.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, described recycle stream dynamic formula bioreactor system
In the transparent shape pipeline of each cylinder in system, built-in automatic cleaning machine device --- wiper, it is ensured that " recycle stream
It is dynamic " the continuity operation of photo-bioreactor system, no automatic cleaning of dismounting；
The effect of wiper is the formation for preventing from having in PET pipes biomembrane.In order to complete this task, when note in pipeline
When having expired water, each honest and upright official's device will lift the top arranged to corresponding pipe, and be required to return to bottom when pipe row is sky.
The body part of wiper is filled with the cylinder (part A) of foam, and sufficiently large volume makes its buoyancy be enough to make it in pipe
With water level rise and fall in row.With the rising (and decline) of wiper, edge (the B portions of its two rubber seal
Point) remove all algae for being grown in PET pipe walls.But, although the edge that these pads must be arranged with pipe keeps contacting, still
They must can not also limit the current on wiper periphery.In order to prevent such limitation, each pad should have several permissions
The breach that enough current pass through.Angle between the breach up and down that the reserved current of rubber sheet gasket pass through up and down is 45 degree, works as water
By when, rotate wiper in the presence of current turn of speed, to ensure that transparent pipeline inside tube wall seamless cleans its all face
Product.Each wiper can also while two also has a porous disk (C portion) to prevent wiper from departing from pipe row
Micro algae growth liquid is set to flow into and flow out at the top (and bottom) of manifold, respectively as shown in Figures 2 and 3.Finally, whole wiper
Accessory be one piece (D is with E parts) by screw rod and nut group.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, described recycle stream dynamic formula bioreactor gram
Product in the prior art has been taken to lack temperature, illumination, mix small uneven, volume, easy microbiological contamination, difficult in maintenance, extraction difficulty etc.
The other bioreactor produced problems of almost all, realize automation closed culture.Recycle stream dynamic formula photo-biological is anti-
Answer device system architecture clearly demarcated, each unit plate has the ability of certain scale and continuous culture microalgae, and single follows
The expense produced in circulation dynamic formula bioreactor independent operating is low.
In the recycle stream dynamic formula photo-bioreactor system of the present invention, described recycle stream dynamic formula bioreactor system
System is best suitable for extensive industrialization.The each square formation of recycle stream dynamic formula photo-bioreactor system is that multiple units are combined connection
Form, with the big small-scale change in soil, operation can be gradually extended in batches.Reactor dismounting is simple, convenient transport, can be
Built in the sufficient environment of any illumination, after the completion of building run and safeguard it is proper in the case of service life it is very long.
Corner angle are not present in the recycle stream dynamic formula photo-bioreactor system of the present invention, and the arrangement of the transparent shape pipeline of its cylinder connects
Connect mode, the algae that can be allowed in closed system does not have the dead angle of inactive state, this to clean, sterilize become it is simpler,
Convenient, the agitation of bioreactor culture liquid more fully and is evenly distributed, so as to substantially increase culture efficiency, independent is automatic clear
Cleaning device is designed, and will not also increase cleaning difficulty with the increase of height；The energy is few, expense is low for consumption in the process of running,
And in addition to cylindrical pipe threeway junction, all it is made of the polyethylene terephthalate of translucency.
The recycle stream dynamic formula photo-bioreactor system collection service life length of the present invention, capacity are big, operating cost is low, most suitable
Preferably extensive industrialization is；Solve to overcome cleaning dead angle, glass wall pressure, reactor that existing reactor is also present high
The some problems such as degree is limited, the mobile inconvenience of assembling, photosynthetic microalgae biological culture cost height, efficiency are low caused by, it is impossible to
The problem of extensive industrialization is cultivated.It is the automation enclosed circulation flowing of optimum microalgae aquaculture industrialized scale
Formula photo-bioreactor system；Particularly cubical content is higher than common closed photo bioreactor algal grown nutrient liquid capacity
As many as 10-20 times, earlier above 50% more than the algal grown nutrient liquid capacity of the snakelike photo-bioreactor system of our company.
Brief description of the drawings
Fig. 1 is recycle stream dynamic formula bioreactor schematic flow sheet of the present invention.
Fig. 2 is wiper assembling schematic diagram of the present invention.
Fig. 3 is the sectional view of wiper of the present invention use state in the duct.
Fig. 4 is recycle stream dynamic formula bioreactor unit connection diagram of the present invention.
Fig. 5 is pH value determination and set-point schematic diagram.
Fig. 6 is dissolved oxygen associated production and PAR schematic diagrames.
Fig. 7 is recycle stream dynamic formula bioreactor circular pipe connection diagram of the present invention.
In Fig. 1, symbol 1 represents hold-up vessel, and symbol 2 represents liquid operation pump, and symbol 3 represents recovery/drainage and returned
Pipeline, symbol 4 represents recovery/recurrence pipe, and symbol 5 represents spilling/liquid, and gas returns pipe, and symbol 6 represents bioreactor,
Symbol 7 represents harvest mouth, and symbol 8 represents harvest storage tank, and symbol 9 represents waste liq/useless nutrient solution, and symbol 10 represents ultraviolet and killed
Bacterium device, symbol 11 represents the biomass product of concentration, and symbol 12 represents recurrent canal, and symbol 13 represents flue gas/carbon dioxide
Imported unit, symbol 14 represents gas operation pump, and symbol 15 represents flue and pH value and dissolved oxygen probe.
In Fig. 2, Fig. 3, symbol A represents lathery cylinder, and symbol B represents rubber seal, and symbol C represents tool
Disk jaggy, symbol D represents screw rod, and symbol E represents nut.
In Fig. 4, symbol 16 represents bioreactor A, and symbol 17 represents bioreactor B, and symbol 18, which is represented, to be connected
Joint, symbol 19 represents connecting tube.
Below in conjunction with accompanying drawing, the present invention will be described in detail
With reference to Fig. 1, the transparent shape pipeline of cylinder and branch pipe tee connection are made up of polyethylene terephthalate, micro algae growth liquid
In blend tank it is deployed after, it is micro- into recycle stream dynamic formula photo-bioreactor system using flowing velocity as 10-20 cels
Algae growth-promoting media rests on each cylindrical transparent pipeline, is uniformly passed through carbon dioxide, carries out photosynthesis；Micro algae growth liquid stops
The time is stayed, is within about 6-12 hours a cycle period according to the difference of microalgae seed；Return in micro algae growth liquid pool and supplement light
The nutriment required for microalgae biological growth is closed, and removes harmful substance.
With reference to Fig. 7, the selection of material and chain joint assembly：Thin-walled PET is managed, and the material of packaging is commonly used for, with US spec 40
Polyvinyl chloride pipe fitting constitutes the main body of bioreactor, and thinner transverse tube above is the exhaust under system quiescence state
Pipe；Here is cylindrical pipe threeway, the transparent shape pipeline of cylinder, and the bottom of connector or top I-steel are branch pipe tee connection handle
It is multiple to circulate pipeline support and in parallel, parallel, the two behavior units arrangement that is fixed up, overlook head and the tail connection extension and communicate.
Seal water-tight to ensure that the transparent shape pipeline of cylinder is connected with branch pipe tee connection, appropriately sized elastic tape is used as creating pipeline
Pad between accessory.Sealing is reinforced using a worm-drive clip.Vertical pipe be by prefabricated I-shaped steel frame,
Parallel connection is overlooked head and the tail connection extension and communicated by bottom I-steel, supports and is fixed up, keeps the stability of system；Top is also
Fixed by I-steel, without being suspended from top.
With reference to Fig. 5, carbon dioxide is injected according to the pH value of system.When carbon dioxide injection is to reactor, carbon will be formed
Acid (carbon dioxide solubility generates carbonic acid in water), then reduces the pH value of algae.When microalgae biology after photosynthesis, dissolving
Carbon dioxide is consumed, and the pH value of system rises.This mode can make the pH value of system be maintained at most suitable algal grown
Value range (pH=4.2-6.2), while enough carbon dioxide can be provided again to maintain growth.This point is particularly significant, especially
Contain other acidic flue gas compositions such as SOx and NOx in nutriment gas.Especially SOx lysate can form sulfuric acid, make culture medium
It is peracid, so as to suppress growth.For this reason, SOx will not be added into the culture systems that algae utilizes.
Fig. 5 describes the method for control ph, the carbon dioxide stream for adjusting the marine alga of 650 liters of pipelines in six days
Amount.Horizontal line is the pH set points for representing reactor, and it is the pH value of systematic survey that trace line, which is represented,.This figure also captures production
The generation of the breathing of raw carbon dioxide, so as to reduce the pH value at night.
With reference to Fig. 6, except pH value, also constantly monitoring tracking photo-biological is anti-among running for number of different ways
Answer the performance of device and the health of algae.These metering systems (including pH value) monitor nutriment tank and PBR by a series of probe
Between working fluid.Environment is all by the systematic function measurement related with ambient influnence to the temperature of process.In the gas phase
Carbon dioxide content is tracked by carbon dioxide detector, to ensure that system has enough carbon dioxide to go to guide the life of algae
It is long.If desired, photosynthetically active radiation (PAR) can use quantum sensor, measurement photon quantifies in photosynthetic spectrum flux.Dissolving
Lambda sensor is the product of the photosynthetic response for tracking, it is ensured that dissolved in systems without excessive oxygen.The generation of oxygen
It is equal to the consumption of carbon dioxide.Therefore, by monitoring dissolved oxygen, performance and the health of algae be can confirm that.Fig. 6 is shown
Dissolved oxygen and the relation of PAR values in bioreactor.
It is worth noting that, oxygen inhibiting rate can be by the energy of the solvent absorbing carbon dioxide in alga cells
The productivity of power interference limitation algae.However, when the carbon dioxide source using flue gas, problem just becomes very little, because
Carbon dioxide is as the gas after burning, its oxygen content compared to little air 80%.
Claims (9)
1. a kind of recycle stream dynamic formula photo-bioreactor system, it is characterised in that including hold-up vessel (1), liquid operation pump (2),
Recovery/drainage returns pipeline (3), recovery/recurrence pipe (4), spilling/liquid, gas recurrence pipe (5), bioreactor (6), receipts
Obtain mouth (7), harvest storage tank (8), waste liq/useless nutrient solution (9), ultraviolet sterilization apparatus (10), the biomass product of concentration
(11), recurrent canal (12), flue gas/carbon dioxide imported unit (13), gas operation pump (14), flue and pH value and dissolved oxygen
Probe (15)；Wherein deployed nutrient solution is put into hold-up vessel (1), running pump (2) by liquid conveys nutrient solution
Enter to the lower section of bioreactor (6), introduce purification flue gas/carbon dioxide while running pump (14) by gas
Microalgae in bioreactor is carried out after the completion of photosynthesis, photosynthesis, and cultivating microalgae density saturated solution is by reclaiming/drawing
Flow back to and return pipeline (3), in the case where liquid runs the dynamic action of pump (2) by recovery/recurrence pipe (4), enter from harvest mouth (7) and receive
Obtain in storage tank (8), solid-liquor separation removes the biomass product (11) of concentration, and remaining waste liq/useless nutrient solution (9) is by ultraviolet
The nutrient solution that given up after line sterilizing unit (10) sterilization is used after returning in hold-up vessel (1) nutrition deployment again；From optical-biological reaction
The gas and liquid that the top of device (6) is overflowed return pipe (5) by spilling/liquid, gas and also return to middle storage through recurrent canal (12)
Used again after nutrition deployment in groove (1)；Wherein described bioreactor (6) has and saturating into multiple transparent columns of two rows
Bright shape pipeline is constituted, and the upper and lower part of the transparent shape pipeline of cylinder connects insertion, every group of bioreactor unit by threeway
It is made up of two bioreactors for being staggered at 45 ° (6), the upper and lower part of two bioreactors (6) is mutual
Connect into an inlet and outlet；There is an air shooter line in the bottom communicating pipe of described bioreactor (6), it is described
Air shooter line on gather it is multiple for gas escape apertures；
Wherein：It is built-in in the transparent shape pipeline of each cylinder of described bioreactor (6) that wiper is installed, it is described
Wiper includes lathery cylinder, pad, two rubber seal, two tool disk rubber sheet gaskets jaggy and two
Individual have a porous disk, and lathery cylinder is sequentially connected rubber seal, has disk rubber jaggy up and down
Glue gasket, pad and with porous disk, pass through screw rod and nut connection shaping.
2. recycle stream dynamic formula photo-bioreactor system according to claim 1, it is characterised in that in wiper, have
There are several breach for allowing enough current logical on the disk rubber sheet gasket of breach, it is upper that the current that rubber sheet gasket is reserved up and down pass through
Angle between lower breach is 45 degree, rotates wiper in the presence of the current turn of speed that water passes through, to ensure transparent pipeline
Inside tube wall seamless cleans its all area.
3. recycle stream dynamic formula photo-bioreactor system according to claim 1, it is characterised in that described photo-biological is anti-
Answer device unit：Bottom, top I-steel support branch pipe tee connection and multiple transparent pipelines that circulate and fixed by snap
Get off, and it is in 90 ° with ground, there is the rubber ring for preventing leakage between connector between the transparent shape pipeline of cylinder and branch pipe tee connection, pass through
Buckle is fixed.
4. recycle stream dynamic formula photo-bioreactor system according to claim 1, it is characterised in that described photo-biological is anti-
Device is answered by 20-500000 cylindrical transparent pipeline and branch pipe tee connection, I-steel, connector, support frame and fixation constitutes photoproduction
The series connection of thing reactor unit is embarked on journey, parallel, two behavior units arrangement, is cascaded, and is overlooked head and the tail connection extension and is communicated, constitutes
Recycle stream dynamic formula bioreactor；Cylindrical transparent pipeline is highly 0.80 meter -6.00 meters, a diameter of 0.05 meter -0.55 meter.
5. recycle stream dynamic formula photo-bioreactor system according to claim 1, it is characterised in that multiple optical-biological reactions
The series connection of device unit is embarked on journey, parallel, two behavior units arrangement, is cascaded, and is overlooked head and the tail connection extension and is communicated, constitutes recycle stream
Dynamic formula bioreactor；Multiple recycle stream dynamic formula bioreactors, then be connected in parallel and constitute recycle stream dynamic formula photoproduction
Thing reactor assembly.
6. the application method of recycle stream dynamic formula photo-bioreactor system according to claim 1, it is characterised in that microalgae
After growth-promoting media is deployed in blend tank, recycle stream dynamic formula bioreactor system is entered by 10-20 cels of flowing velocity
System, micro algae growth liquid rests on each cylindrical transparent pipeline, is uniformly passed through carbon dioxide, carries out photosynthesis, microalgae life
The long liquid residence time, 8-16 hours is a cycle period according to the difference of microalgae seed；When probe (15) detects microalgae liquid
During body subalimentation, as required for importing and exporting and microalgae liquids recovery to nutrient solution allocated the appropriate micro algae growth of storage tank, supplement
Nutriment, into next microalgae photobiological reaction cycle；Pump is run by liquid micro algae growth liquid is squeezed into photo-biological
Reactor assembly, fully absorbs photosynthesis microalgae biology；It is furnished with titanium dioxide in the front end of photo-bioreactor system device
Carbon air accumulator, allotment storage tank, intermediate storage tank, harvest storage tank, in blend tank microalgae seed, plant growth nutrient solution together
Photo-bioreactor system is pumped into, is then turned on being passed through the carbon dioxide control valve of the transparent shape pipeline of cylinder, titanium dioxide is supplemented always
Carbon, remains carbon dioxide and in pH value setting range, allows microalgae biology to keep optimum growh state to carry out photosynthetic in nutrient solution
Effect；Microalgae biological growth liquid is returned in blend tank in time according to Testing index the nutrition for supplementing microalgae biological growth, when
When microalgae liquid reaches setting density, micro algae growth liquid enters harvest storage tank, can be collected automatically by collector, obtains both culturing microalgae
Product.
7. the application method of recycle stream dynamic formula photo-bioreactor system according to claim 6, it is characterised in that microalgae
Growth-promoting media enters recycle stream dynamic formula photo-bioreactor system, and micro algae growth liquid is rested in each cylindrical transparent pipeline, manages
The air admission hole upstream end of road threeway bottom is connected to the inlet manifold of autocontrol valve, and unlatching is passed through the two of the transparent shape pipeline of cylinder
Carbonoxide control valve, supplements carbon dioxide always, and when the pH value of slurry is higher or lower than setting range, gas control valve regulation increases
The amount for few carbon dioxide supplement that adds deduct, in pH value setting range, remains the most appropriate solubility of carbon dioxide, allows microalgae to give birth to
Thing keeps optimum growh state to carry out photosynthesis.
8. the application method of recycle stream dynamic formula photo-bioreactor system according to claim 6, it is characterised in that use cigarette
Road and pH value and dissolved oxygen probe (15) are close to determine microalgae in the liquid in hold-up vessel, blend tank and the transparent shape pipeline of cylinder
Degree, carbon dioxide content in the gas phase is tracked by carbon dioxide sensor, to ensure that system has enough carbon dioxide to go
The growth of algae is guided, photosynthetically active radiation can use quantum sensor, and measurement photon quantifies in photosynthetic spectrum flux, dissolved oxygen
Sensor is the product of the photosynthetic response for tracking, it is ensured that dissolved in systems without excessive oxygen.
9. the application method of recycle stream dynamic formula photo-bioreactor system according to claim 6, it is characterised in that when up to
Algae harvest is carried out during to setting density, microalgae liquid enters harvest storage tank by importing and exporting.
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| CN201510284884.2A CN105039134B (en) | 2015-05-28 | 2015-05-28 | Recycle stream dynamic formula photo-bioreactor system |
| PCT/CN2015/090215 WO2016187996A1 (en) | 2015-05-28 | 2015-09-22 | Circular flow type photobioreactor system |
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| CN105039134B (en) * | 2015-05-28 | 2017-08-25 | 连衡会投资有限公司 | Recycle stream dynamic formula photo-bioreactor system |
| US20170355942A1 (en) * | 2016-06-13 | 2017-12-14 | University Of Kentucky Research Foundation | Cyclic photobioreactor and method for biofilm control |
| CN107022474A (en) * | 2017-06-12 | 2017-08-08 | 李永亮 | A kind of wiper and microalgae culturing device adopting |
| AU2018313189A1 (en) * | 2017-08-08 | 2020-03-26 | Forelight, Inc. | Photosynthetic bioreactor for the conversion of electricity and fertilizer into biomass |
| CN107475090B (en) * | 2017-09-19 | 2019-06-14 | 广西大学 | A kind of tidal type bioreactor |
| CN107475089B (en) * | 2017-09-19 | 2019-06-14 | 广西大学 | A kind of tidal type photo-biological culture apparatus |
| CN107400626B (en) * | 2017-09-19 | 2019-05-17 | 广西大学 | A kind of micro algae culturing device |
| CN109337820A (en) * | 2018-12-10 | 2019-02-15 | 中国石油化工股份有限公司 | The method of continuous production microalgae |
| FR3098828B1 (en) * | 2019-07-19 | 2021-08-27 | Centralesupelec | Device and method for producing photosynthetic microorganisms in a photobioreactor |
| CN113186079A (en) * | 2021-05-13 | 2021-07-30 | 云南爱尔发生物技术股份有限公司 | System for realizing production of circulating liquid flow in microalgae culture photobioreactor |
| CN113403188A (en) * | 2021-06-17 | 2021-09-17 | 刘正君 | Anti-pollution circulation runway formula culture system based on algae is cultivated |
| CN113663506A (en) * | 2021-07-28 | 2021-11-19 | 中铁时代建筑设计院有限公司 | Biological carbon capture device based on cast-in-place major diameter excavation supporting tubular pile |
| CN113699034A (en) * | 2021-08-31 | 2021-11-26 | 松山湖材料实验室 | Closed microorganism culture system |
| CN114106992A (en) * | 2022-01-05 | 2022-03-01 | 山西农业大学 | Device for improving utilization efficiency of microalgae carbon dioxide |
| CN114368838A (en) * | 2022-01-11 | 2022-04-19 | 苏州科特环保股份有限公司 | Treatment method of nitrogen and phosphorus wastewater |
| WO2024033597A1 (en) * | 2022-08-12 | 2024-02-15 | Grossman Scott Oliver | Gas processing device |
| CN118272196B (en) * | 2024-06-04 | 2024-08-27 | 江苏省沿海开发投资有限公司 | Real-time monitoring method and equipment for photo-bioreactor |
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| AU2008261616A1 (en) * | 2007-06-14 | 2008-12-18 | Roger Stroud | Apparatus and method for the culture of photosynthetic microorganisms |
| CN102533528B (en) * | 2012-01-12 | 2013-04-24 | 天津大学 | Sealed continuous culture experimental apparatus based on simulation of microalgae amplification culture |
| CN102660448A (en) * | 2012-04-27 | 2012-09-12 | 天津大学 | Sleeve type photobiological reaction system for culturing microalgae on scale by utilizing waste gas and waste heat |
| CN103396950B (en) * | 2013-08-09 | 2016-01-06 | 烟台大学 | A kind of natural pond liquid ecological purification method based on both culturing microalgae |
| CN103525686B (en) * | 2013-10-24 | 2015-01-21 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
| CN103602579B (en) * | 2013-11-27 | 2015-03-04 | 青岛旭能生物工程有限责任公司 | Pipeline-type photobioreactor for scale culture of microalgae |
| CN104513794A (en) * | 2015-01-26 | 2015-04-15 | 连衡会投资有限公司 | S-shaped photobioreactor system |
| CN105039134B (en) * | 2015-05-28 | 2017-08-25 | 连衡会投资有限公司 | Recycle stream dynamic formula photo-bioreactor system |
| CN204779556U (en) * | 2015-05-28 | 2015-11-18 | 连衡会投资有限公司 | Circulatory flow dynamic formula photobioreactor system |
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| CN105039134A (en) | 2015-11-11 |
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