Snakelike photo-bioreactor system
Technical field
The utility model relates to a kind of bioreactor, relates to one especially and cultivates multiple photosynthetic microorganism by photosynthesis, carry out the snakelike photo-bioreactor system of industrialization cultivation.
Background technology
The growth and breeding of photosynthetic microorganism, especially rich oil energy microalgae, Haematocoocus Pluvialls, green alga, blue-green algae etc. (being called for short algae) mainly relies on corresponding mineral substance.Single celled algae has extremely strong fecundity, can absorb a large amount of carbonic acid gas under photosynthesis.Algae by photosynthesis, utilizes the cycling of elements such as luminous energy, water, mineral substance and carbonic acid gas to become to be rich in a large amount of grease, protein, astaxanthin, the contour attached value organic compound of spirulina.
Cultivate different alga microbials by bioreactor, multiple different high value added product can be gathered in the crops.And because algae has the fast nutritive substance such as absorbing carbon dioxide, nitrogen, phosphorus be translated into the ability of organic compound (as grease, albumen, astaxanthin of being stored in cell) from surrounding environment, it can also be used for purifying liquid waste and recycle the rich carbonated stack gas of thermal power plant and carry out environmental protect.Algae can play the integral part in biopurification process in systems in which, after treating maturation, through system process such as extractions, output large number of biological fuel (as biofuel, alcohol and methane etc.), its frond also can be used as the important source material of animal-feed and fertilizer and improvement soil.In algal culture, its economic benefit produced is decided by the scale of the selection of algae, cultivation, the application efficiency of bioreactor.
Domestic at present have the bioreactor of scale to be generally open pond, transverse tube, transparent light film etc., builds up 1000 ~ 5000 square metres of annular shallow ponds (depth of water 15 ~ 30 centimetres) more, with manpower or paddle wheel, nutrient solution is circulated.This production model has significant limitation, its structure is simple, although there is the advantage of investing low easy construction, but same efficiency is too low, algae grows out of doors a lot of uncontrollable factor, lacks temperature, illumination, mixing is uneven, volume is little, contact be easy to microbiological contamination with air as open pool cultivated, make overall productivity low, often cause cultivating unsuccessfully.
Open defect has promoted the development of closed culture systems, has occurred with the closed photo bioreactor that transparent transverse tube or other container are made.The application of transverse tube type reactor is also subject to its high cost, inefficient restriction, only for the production of short run, high added value Special Products; The bioreactor of other kind also because of many reasons as extracted difficulty, construction cost is high, volume is little, cleaning is difficult etc. and in succession cannot produce economic benefit by large-scale farming.
Patent document (publication number WO2008/151376A1) discloses a kind of film reactor, but this film reactor also exists following shortcoming:
The structural unit size of a, reactor cannot adjust after determining;
B, manufacture craft are comparatively complicated, and each film unit side is wavy, need hot pick or other auxiliary processes;
C, because reactor width is less, simultaneously wavy sidewalls causes internal run-through poor, has dead angle, cleans very inconvenient;
D, because this reactor side is main sensitive surface, reactor pose pattern (direction) is restricted;
E, reactor are comparatively dark, and the stress distribution on side plate is uneven, and material top mechanical property utilization ratio is low.Meanwhile, the material performance requirement of hydraulic pressure to membrane wall is higher.Material cost increases.
Summary of the invention
One of the purpose of this utility model is to provide a kind of snakelike bioreactor.
Two of the purpose of this utility model is to provide a kind of snakelike photo-bioreactor system.
These and other objects of the present utility model will be come to explain further by the following detailed description and explanation.
Snakelike photo-bioreactor system of the present utility model, comprise process tank, detector, process pump, bioreactor, transfer line, oil return menifold, results storage tank, wherein: pass through transfer line, process tank is connected with bioreactor by process pump, results storage tank is entered by oil return menifold to harvest mouth from bioreactor product out, enter process tank from results storage tank liquid out by ultra-violet sterilization to carry out nutrient and give back, process tank is entered by head space circulation from the carbonic acid gas of harvest mouth gas out and interpolation, the amount that process tank enters bioreactor is controlled by detector.
In the utility model, said bioreactor is snakelike, with snakelike arrangement mode, row with to comprise multiple pipeline in the ranks and be all connected in the serpentine path that straight line extends, comprise transparent cylindrical pipe and bracing frame, cylindrical pipe is connected with bracing frame, is interconnected between bracing frame, and in 90 ° with ground; The upper and lower of two cylindrical pipes is connected by " U " type web member, the bottom of " U " type web member or top are supported each " U " pipe be fixed up by bottom or top joist steel, form snakelike bioreactor by many cylindrical pipes after " U " type web member connects.
In the utility model, have the rubber ring preventing from revealing, fixed by buckle between cylindrical pipe and " U " type web member, opening is arranged at bottom described " U " type web member or top, and the upper opening of each " U " type web member is all connected with vapor pipe.Multiple snakelike bioreactor is interconnected the snakelike bioreactor unit of a formation standard, the snakelike bioreactor unit of multiple standard is interconnected and forms snakelike bioreactor, except junction, it adopts the material of whole printing opacity, and described snakelike bioreactor has import, outlet and thief hole.With support frame supports outside two long side surfaces of serpentine tube, support frame is evenly laid along serpentine tube, and the height of support frame is not less than the height of serpentine tube; Support frame vertical ground, the rectangular rectangle in every side, the rectangular vertical edges in right angle is support frame column, is serpentine tube between support frame column.Snakelike bioreactor structure is clearly demarcated, and each unit has the ability of certain scale and the micro-algae of cultured continuously, and the expense produced in independent operating is low.Described bioreactor is made up of 20-500000 cylindrical pipe and U-shaped pipe, be preferably and be made up of 500-50000 cylindrical pipe and U-shaped pipe, the height of cylindrical pipe (transparent) and U-shaped pipe is 1.25 meters-4.20 meters, diameter is 0.07 meter-0.30 meter, is specifically determined by the kind difference of cultivating microalgae.Cylindrical pipe is made up of polyethylene terephthalate, and is transparent.The velocity of flow of photo bio nutrient solution is 10-20 cel, each photo bio nutrient solution residence time by snakelike bioreactor transparent pipeline is 10-20 minute, it within 1 to 2 hour, is a loop cycle, partly stop half operation interval, cultivate in liquid pool the nutritive substance supplemented required for photosynthetic microorganism growth in photo bio, and remove objectionable impurities.
In snakelike bioreactor of the present utility model, by process pump, photo bio nutrient solution is squeezed into bioreactor, photosynthetic microorganism is fully absorbed, blend tank is furnished with in the front end of photo-bioreactor system device, first in blend tank the nutritive medium of micro-algae seed, plant-growth, the saturated lysate of carbonic acid gas pumps into bioreactor together, allows micro-algae plant carry out photosynthesis; Supplement the nutritive medium of micro-algae plant-growth in time according to Testing index in blend tank and supplement carbonic acid gas always, remain the saturated lysate of carbonic acid gas, keep the optimum growh state of micro-algae biology; The circulation repeatedly in bioreactor of photo bio nutrient solution supplements, flow in unit successively, from the initial beginning, flowing out to the last recycle system flows in photo bio cultivation liquid pool again, realize the stirring cycling and reutilization of photo bio nutrient solution, when reaching setting density, collector can be collected automatically, obtains both culturing microalgae product.
In snakelike photo-bioreactor system of the present utility model, photo bio nutrient solution is introduced into cylindrical pipe again to oil return manifold by process pump, oil return manifold is by the flow set out of each pipeline and turn back to charging stock tank, when being back to the top of charging stock tank, by T-shaped tube fittings 11 (Fig. 3) formed suction flow to be under, introduce gas and enter system, carbonic acid gas enters blend tank with the velocity of flow same with phegma along with phegma, the mixing that two-phase lubrication natural energy is extraordinary and mass transfer, thus in growth promoting effects medium, dissolve consumption carbonic acid gas.The suction side returning pipe arrangement is connected to the intake manifold of automatically controlled valve, and when the pH value of slurry is higher than setting point, control valve (A) is connected to CO 2 raw material gas and opens, and allows gas to be introduced in feed chute; When pH value is lower than setting point, air intake control valve is closed, and another control valve (B) is opened, and air is recycled at headspace, and exhaust system prevents carbonic acid gas at headspace; 3rd control valve (C) adds system to as safety valve and prevents nutriment tank from becoming pressurization flue gas, and in this fashion, carbonic acid gas is consumed by algae, and extra carbonic acid gas automatic input system, to maintain the pH value of action required.According to the pH value injecting carbon dioxide of system, when carbon dioxide injection is to reactor, carbonic acid will be formed, then the pH value of algae is reduced, when micro-algae is biological, after photosynthesis, the carbonic acid gas of dissolving is consumed, and the pH value of system rises, this mode can make the pH value of system remain on the value range of the most applicable algal grown, and enough carbonic acid gas can be provided again to maintain growth simultaneously.
In snakelike photo-bioreactor system of the present utility model, the fluxion in process tank and cylindrical pipe is measured with probe a series of on detector, carbon dioxide content is in the gas phase followed the tracks of by CO2sensors, and with the CO2sensors dissolved to guarantee that system has enough carbonic acid gas to go to guide the growth of algae, photosynthetically active radiation can use quantum sensor, measurement of photon quantizes at photosynthetic spectrum flux, dissolved oxygen sensor is used to the product of the photosynthetic response of following the tracks of, and guarantees do not have excessive oxygen dissolution in systems in which.Algae results are by harvest mouth to an independent collection box, from concentrating algae by standard manner here, as flocculation, precipitation, centrifugation and flotation etc.Photo bio nutrient solution in systems in which circulation repeatedly supplements, and flows successively in each square formation, from the initial beginning, flows out and again flows into photo bio and cultivate in liquid pool, realize the stirring cycling and reutilization of photo bio nutrient solution to the last recycle system.When reaching setting density, collector can be collected automatically, obtains both culturing microalgae product.
Snakelike photo-bioreactor system of the present utility model overcomes that product in prior art lacks temperature, illumination, mixing is uneven, volume is little, easy microbiological contamination, difficult in maintenance, extract difficulty and wait almost all other bioreactor produced problem, achieve automatization closed culture." snakelike " bioreactor structure is clearly demarcated, and each unit has the ability of certain scale and the micro-algae of cultured continuously, and the expense produced in independent operating is low.
Snakelike photo-bioreactor system of the present utility model is when extensive industrialization, and floor space is very large, and the increase of volume can increase cost of manufacture and the difficulty of reactor; The each square formation of snakelike bioreactor is that multiple unit carries out being connected forming, and along with the large small-scale change in soil, successively can extend operation in batches.Reactor dismounting is simple, and convenient transport, can build in the environment of any illumination abundance, after having built, when operation and maintenance is proper, work-ing life is very long.
There are not corner angle in snakelike photo-bioreactor system of the present utility model, the arrangement mode of connection of its " U " type, the algae in closed system can be allowed there is no the dead angle of stationary state, this makes to clean, sterilize the more convenient of change, the stirring of bioreactor culture liquid is more abundant and be evenly distributed, thus substantially increase culture efficiency, and along with height increase also can not increase cleaning difficulty.In operational process, consume the little expense of the energy low, and except " U " type junction whole printing opacity.
Snakelike bioreactor of the present utility model and system thereof, solve and overcome that cleaning dead angle that existing reactor also exists, glass wall pressure, height for reactor are limited, the mobile some problem such as inconvenient of assembling, thus the photosynthetic microorganism toxigenic capacity caused is high, efficiency is low, cannot on a large scale industrialization cultivation problem.
Accompanying drawing explanation
Fig. 1 is the snakelike bioreactor schematic diagram of the utility model.
Fig. 2 is the snakelike bioreactor connection diagram of the utility model.
Fig. 3 is the snakelike bioreactor operation chart of the utility model.
Fig. 4 is serpentine tube assembly schematic diagram in the snakelike bioreactor of the utility model.
In FIG, symbol 1 represents outlet, and symbol 2 represents import.
In figs. 2 and 3, symbol 1 represents process tank, symbol 2 represents detector, symbol 3 represents process pump, symbol 4 represents loop shunting, and symbol 5 represents transfer line, and symbol 6 represents phototube row, symbol 7 represents oil return menifold, symbol 8 represents harvest mouth, and symbol 9 represents gas entrainment, and symbol 10 represents head space circulation, symbol 11 represents carbonic acid gas to be added, symbol 12 representative returns process tank, and symbol 13 represents results storage tank, and symbol 14 represents nutrient and gives back, symbol 15 represents ultra-violet sterilization, and symbol 16 represents removes concentrated biomass.
In figure 3, symbol A, B, C represents control valve.
With reference to figure 2, photo bio nutrient solution is incorporated into PET pipe by manifold out by impeller pump.Generally remain in the scope of 10-20 cel by the velocity of flow of each pipe, each photo bio nutrient solution by PBR bioreactor transparent pipeline provides the residence time of about 10-20 minute.At the end of PBR, to combine common flowing turn back to process tank at each parallel lines of manifold.When being back to the top of process tank, being formed under suction flows to and be, introduce gas simultaneously and enter system by T-shaped tube fittings, carbonic acid gas enters process tank along with the velocity of flow that phegma is same with phegma.The mixing that two-phase lubrication natural energy is extraordinary and mass transfer, thus in growth promoting effects medium, dissolve consumption carbonic acid gas.Use such system to utilize the Infrastructure of pipeline and impeller pump extremely to put in place, can well produce a desired effect.
With reference to figure 3, describe nutriment tank and associated conduit comprises return line.The suction side returning pipe arrangement is connected to the intake manifold of automatically controlled valve.Open when the pH value of slurry is connected to CO 2 raw material gas higher than setting point (such as pH7.0) control valve (A), allow gas to be introduced in feed chute.When pH value is lower than setting point, air intake control valve is closed, and another control valve (B) is opened, and air is recycled at headspace, and exhaust system prevents carbonic acid gas at headspace.3rd control valve (C) adds system to as safety valve and prevents nutriment tank from becoming pressurization flue gas.In this fashion, carbonic acid gas is consumed by algae, and extra carbonic acid gas automatic input system, to maintain the pH value of action required.
With reference to figure 4, the selection of material and chain joint assembly: thin-walled PET manages, commonly use the material into packaging, form the main body of bioreactor with US spec 40 polyvinyl chloride tube fittings, transverse tube is above the vapor pipe under system quiescence state; To be " U " type pipe be here between series unit is connected in parallel mouth.Water-tight in order to ensure sealing, the rubber ring of suitable size is used as creating the pad between pipeline and accessory.A worm drive clip is used to reinforce sealing.Vertical ducting is by prefabricated joist steel framework, and bottom joist steel supports each " U " pipe be fixed up, the stability of keeping system; Top is also fixed by joist steel, instead of is suspended on top.The weight of water can be made to become the force of compression of reactor from bottom support reactor, thus keep together, instead of attempt to tear up it.