CN103548565A - Shiitake cultivating and inoculating method - Google Patents
Shiitake cultivating and inoculating method Download PDFInfo
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- CN103548565A CN103548565A CN201310515076.3A CN201310515076A CN103548565A CN 103548565 A CN103548565 A CN 103548565A CN 201310515076 A CN201310515076 A CN 201310515076A CN 103548565 A CN103548565 A CN 103548565A
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Abstract
The invention discloses a shiitake cultivating and inoculating method and belongs to the technical field of mushroom cultivation. The shiitake cultivating and inoculating method is characterized by comprising the following steps of: 1, filling a shiitake cultivating culture medium in fungus bags, enabling the fungus bags to be cylindrical, placing the fungus bags in a sterilization room for sterilization, and cooling to a room temperature; 2, moving the fungus bags in an inoculation room for inoculation; 3, forming rectangular holes in the axial direction of the centers of the two end faces of each cylindrical fungus bag in the inoculation room, inserting shiitake strains with rectangular sections in the rectangular holes, and sealing the holes by using cotton plugs or sponge, wherein the length of each rectangular hole is 2 cm to 3 cm greater than that of each rectangular shiitake strain, the width of each rectangular hole is 0.2 cm to 0.3 cm greater than that of each rectangular shiitake strain, and the height of each rectangular hole is 0.2 cm to 0.3 cm greater than that of each rectangular shiitake strain; 4, moving the inoculated fungus bags in a fungus culturing room for mycelium cultivation. According to the invention, contact space between the strains and the culture mediums in the fungus bags is suitable, the fungus growing speed is fast, and the inoculation efficiency is high.
Description
Technical field
The present invention relates to mushroom-cultivating technical field.
Background technology
Existing cultivating champignon inoculation method is to pack medium into bacterium bag, swing in sterilizing indoor sterilizing, and then bacterium bag is moved into cooling chamber, during inoculation, take to beat cave inoculation method, vaccinator lays out operating desk by bacterium bag, manual equidistant spherical cavity hole of getting 3-4 diameter 1.5cm, degree of depth 6cm on bacterium bag face, with hand, Saw-dust being broken into piece fills in each hole, cave and compresses, then bagging or seal inoculation cave with adhesive tape, inoculation completes, then bacterium bag is moved into bacteria chamber carries out Mycelium culture.The drawback of the method is: pollution probability is high; The gap of bacterial classification and bacterium bag culture medium is large, and a bacterium time is long and inoculation efficiency is low.
Summary of the invention
This technical problem to be solved is to provide a kind of cultivating champignon inoculation method, have medium gap in bacterial classification and bacterium bag suitable, send out that bacterium speed is fast, mycelia is difficult for the feature aging, inoculation efficiency is high.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of cultivating champignon inoculation method, is characterized in that operating as follows:
One, pack cultivating champignon medium into bacterium bag, described bacterium bag forms cylinder, and bacterium bag is put into sterilizing chamber's sterilizing cool to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, in the axial of center of cylinder bacterium bag both ends of the surface, beat rectangular opening, in described rectangular opening, insert the mushroom strain that cross section is rectangle, and seal with tampon or sponge; The length of described rectangular opening is greater than the mushroom strain length 2cm-3cm of rectangle, and the width of rectangular opening is greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, postvaccinal bacterium bag is moved into bacteria chamber and carry out mycelium cultivation.
The further improvement that the present invention is done is:
The mushroom strain of described rectangle expands numerous technique with conventional edible fungus species to be made, and its material is broadleaf tree, specification: 12cm-18cm, wide 0.6cm-0.8cm, high 0.2cm-0.4cm.
Described bacterium bag is the knuckle bag of polyethylene or polypropylene material, the specification of bacterium bag after charging: long 40cm-45cm, and directly through 10cm-12cm.
Described rectangular opening is the rectangular opening going out with the jack machine transplanting of rice through sterilization.
By packed sterilizing chamber's sterilizing the cool to room temperature of pushing on sterilizing frame of described bacterium, the specification of described sterilizing frame: long 150cm, wide 43cm-48cm, high 100cm; Between described sterilizing chamber and transfer room, be provided with telescopic aseptic tunnel, the bottom of described telescopic aseptic tunnel is provided with roller bearing, is provided with ultraviolet sterilizing lamp and ion blower at top; Bacterium bag after sterilizing is moved into transfer room together with sterilizing frame through described telescopic aseptic tunnel to be inoculated.
The beneficial effect that adopts technique scheme to produce is: the method vaccination is few, and pollution probability is low; Chip bacterial classification has been accessed to the shaft centre line position of bacterial spawn, it is most suitable that bacterial classification diffusion grows into the distance at each position in bacterium bag; The method is in tampon or sponge sealing for vaccination, and in mycelial growth process, existing gas permeability can touch sufficient oxygen again, sends out bacterium speed fast, and mycelia is difficult for aging; The method broken bag rate is little, can reduce labor intensity, and saves labour.
Embodiment
Embodiment 1: a kind of cultivating champignon inoculation method, is characterized in that operating as follows:
One, pack cultivating champignon medium into bacterium bag, described bacterium bag forms cylinder, and bacterium bag is put into sterilizing chamber's sterilizing cool to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, in the axial of center of cylinder bacterium bag both ends of the surface, beat rectangular opening, in described rectangular opening, insert the mushroom strain that cross section is rectangle, and seal with tampon or sponge; The length of described rectangular opening is greater than the mushroom strain length 2cm-3cm of rectangle, and the width of rectangular opening is greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, postvaccinal bacterium bag is moved into bacteria chamber and carry out mycelium cultivation.
The mushroom strain of described rectangle expands numerous technique with conventional edible fungus species to be made, and its material is broadleaf tree, specification: 12cm-18cm, wide 0.6cm-0.8cm, high 0.2cm-0.4cm.
Embodiment 2: a kind of cultivating champignon inoculation method, is characterized in that operating as follows:
One, pack cultivating champignon medium into bacterium bag, described bacterium bag forms cylinder, and bacterium bag is put into sterilizing chamber's sterilizing cool to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, in the axial of center of cylinder bacterium bag both ends of the surface, beat rectangular opening, in described rectangular opening, insert the mushroom strain that cross section is rectangle, and seal with tampon or sponge; The length of described rectangular opening is greater than the mushroom strain length 2cm-3cm of rectangle, and the width of rectangular opening is greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, postvaccinal bacterium bag is moved into bacteria chamber and carry out mycelium cultivation.
Described bacterium bag is the knuckle bag of polyethylene or polypropylene material, the specification of bacterium bag after charging: long 40cm-45cm, and directly through 10cm-12cm.
Embodiment 3: a kind of cultivating champignon inoculation method, is characterized in that operating as follows:
One, pack cultivating champignon medium into bacterium bag, described bacterium bag forms cylinder, and bacterium bag is put into sterilizing chamber's sterilizing cool to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, in the axial of center of cylinder bacterium bag both ends of the surface, beat rectangular opening, in described rectangular opening, insert the mushroom strain that cross section is rectangle, and seal with tampon or sponge; The length of described rectangular opening is greater than the mushroom strain length 2cm-3cm of rectangle, and the width of rectangular opening is greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, postvaccinal bacterium bag is moved into bacteria chamber and carry out mycelium cultivation.
Described rectangular opening is the rectangular opening going out with the jack machine transplanting of rice through sterilization.
Embodiment 4: a kind of cultivating champignon inoculation method, is characterized in that operating as follows:
One, pack cultivating champignon medium into bacterium bag, described bacterium bag forms cylinder, and bacterium bag is put into sterilizing chamber's sterilizing cool to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, in the axial of center of cylinder bacterium bag both ends of the surface, beat rectangular opening, in described rectangular opening, insert the mushroom strain that cross section is rectangle, and seal with tampon or sponge; The length of described rectangular opening is greater than the mushroom strain length 2cm-3cm of rectangle, and the width of rectangular opening is greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, postvaccinal bacterium bag is moved into bacteria chamber and carry out mycelium cultivation.
By packed sterilizing chamber's sterilizing the cool to room temperature of pushing on sterilizing frame of described bacterium, the specification of described sterilizing frame: long 150cm, wide 43cm-48cm, high 100cm; Between described sterilizing chamber and transfer room, be provided with telescopic aseptic tunnel, the bottom of described telescopic aseptic tunnel is provided with roller bearing, is provided with ultraviolet sterilizing lamp and ion blower at top; Bacterium bag after sterilizing is moved into transfer room together with sterilizing frame through described telescopic aseptic tunnel to be inoculated.
Claims (5)
1. a cultivating champignon inoculation method, is characterized in that operating as follows:
One, pack cultivating champignon medium into bacterium bag, described bacterium bag forms cylinder, and bacterium bag is put into sterilizing chamber's sterilizing cool to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, in the axial of center of cylinder bacterium bag both ends of the surface, beat rectangular opening, in described rectangular opening, insert the mushroom strain that cross section is rectangle, and seal with tampon or sponge; The length of described rectangular opening is greater than the mushroom strain length 2cm-3cm of rectangle, and the width of rectangular opening is greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, postvaccinal bacterium bag is moved into bacteria chamber and carry out mycelium cultivation.
2. a kind of cultivating champignon inoculation method according to claim 1, it is characterized in that: the mushroom strain of described rectangle expands numerous technique with conventional edible fungus species to be made, and its material is broadleaf tree, specification: long 12cm-18cm, wide 0.6cm-0.8cm, high 0.2cm-0.4cm.
3. a kind of cultivating champignon inoculation method according to claim 1, is characterized in that: described bacterium bag is the knuckle bag of polyethylene or polypropylene material, the specification of bacterium bag after charging: long 40cm-45cm, and directly through 10cm-12cm.
4. a kind of cultivating champignon inoculation method according to claim 1, is characterized in that: described rectangular opening is the rectangular opening going out with the jack machine transplanting of rice through sterilization.
5. a kind of cultivating champignon inoculation method according to claim 1, is characterized in that: by packed sterilizing chamber's sterilizing the cool to room temperature of pushing on sterilizing frame of described bacterium, the specification of described sterilizing frame: long 150cm, wide 43cm-48cm, high 100cm; Between described sterilizing chamber and transfer room, be provided with telescopic aseptic tunnel, the bottom of described telescopic aseptic tunnel is provided with roller bearing, is provided with ultraviolet sterilizing lamp and ion blower at top; Bacterium bag after sterilizing is moved into transfer room together with sterilizing frame through described telescopic aseptic tunnel to be inoculated.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322276A (en) * | 2014-09-26 | 2015-02-04 | 浙江省农业科学院 | Method for improving preparing efficiency and yield and quality of shiitake sticks |
| CN104488558A (en) * | 2014-12-31 | 2015-04-08 | 天津市金三农农业科技开发有限公司 | Method for increasing spawn running rate of mushroom |
| CN104956919A (en) * | 2015-07-08 | 2015-10-07 | 翔天农业开发集团股份有限公司 | Inoculation method for mushroom wood flour liquid strains |
| CN105009944A (en) * | 2015-08-03 | 2015-11-04 | 廊坊市森淼春食用菌有限公司 | Fast inoculation method for mushroom stick |
| CN105145110A (en) * | 2015-07-08 | 2015-12-16 | 翔天农业开发集团股份有限公司 | Mushroom wood powder solid and liquid mixing bacterial strain inoculation method |
| CN107853081A (en) * | 2017-10-27 | 2018-03-30 | 黄兴根 | A kind of inoculation method of mushroom culture medium |
| CN108391557A (en) * | 2018-05-15 | 2018-08-14 | 务川自治县惠农现代农业开发有限公司 | A kind of breeding method of mushroom |
| CN109275492A (en) * | 2018-06-14 | 2019-01-29 | 陕西蓝米信息技术有限公司 | A kind of mushroom implantation methods |
| CN109418025A (en) * | 2017-06-29 | 2019-03-05 | 祁海杰 | A kind of cultivating champignon inoculation method |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010037380A (en) * | 1999-10-16 | 2001-05-07 | 장현유 | Method and Receptacle used to the same for cultivating mushroom |
| DE102006011413A1 (en) * | 2005-03-26 | 2006-10-05 | Basic Holdings | Electric grill for use in open air has serpentine heating element mounted in casing, on top of which grill plates fit which have fat channels with holes which drop fat on to drip pan below the heating element |
| CN102197762A (en) * | 2011-03-30 | 2011-09-28 | 肥东县丰宝种养殖有限责任公司 | Method for producing oyster mushroom |
| CN102577841A (en) * | 2012-03-28 | 2012-07-18 | 常熟市汇康食品厂 | Method for using corncobs to cultivate Agrocybe aegerita |
| CN102027860B (en) * | 2010-12-03 | 2013-01-02 | 福建省农业科学院土壤肥料研究所 | Edible fungus inoculation working system |
| CN202663834U (en) * | 2012-06-21 | 2013-01-16 | 芜湖野树林生物科技有限公司 | Fungi inoculation chamber |
| CN102986450A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Trench cultivation technique for flammulina velutipes |
| CN103250549A (en) * | 2012-02-21 | 2013-08-21 | 天津津金源食用菌专业合作社 | Mushroom cultivation method |
| CN103314741A (en) * | 2013-05-28 | 2013-09-25 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for biologically converting mushrooms by mixed materials of rape stalk |
-
2013
- 2013-10-28 CN CN201310515076.3A patent/CN103548565B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010037380A (en) * | 1999-10-16 | 2001-05-07 | 장현유 | Method and Receptacle used to the same for cultivating mushroom |
| DE102006011413A1 (en) * | 2005-03-26 | 2006-10-05 | Basic Holdings | Electric grill for use in open air has serpentine heating element mounted in casing, on top of which grill plates fit which have fat channels with holes which drop fat on to drip pan below the heating element |
| CN102027860B (en) * | 2010-12-03 | 2013-01-02 | 福建省农业科学院土壤肥料研究所 | Edible fungus inoculation working system |
| CN102197762A (en) * | 2011-03-30 | 2011-09-28 | 肥东县丰宝种养殖有限责任公司 | Method for producing oyster mushroom |
| CN103250549A (en) * | 2012-02-21 | 2013-08-21 | 天津津金源食用菌专业合作社 | Mushroom cultivation method |
| CN102577841A (en) * | 2012-03-28 | 2012-07-18 | 常熟市汇康食品厂 | Method for using corncobs to cultivate Agrocybe aegerita |
| CN202663834U (en) * | 2012-06-21 | 2013-01-16 | 芜湖野树林生物科技有限公司 | Fungi inoculation chamber |
| CN102986450A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Trench cultivation technique for flammulina velutipes |
| CN103314741A (en) * | 2013-05-28 | 2013-09-25 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for biologically converting mushrooms by mixed materials of rape stalk |
Non-Patent Citations (4)
| Title |
|---|
| 于磊等: "袋料香菇栽培关键技术", 《河南林业科技》, vol. 27, no. 4, 15 December 2007 (2007-12-15), pages 50 - 52 * |
| 吴元朝: "香菇菌袋打孔供氧促熟技术", 《食用菌》, no. 6, 15 December 1998 (1998-12-15), pages 42 * |
| 彭学文等: "夏季代料香菇高效优质栽培关键技术", 《食用菌》, no. 3, 23 May 2010 (2010-05-23), pages 38 - 39 * |
| 李威: "玉米芯栽培香菇技术", 《食用菌》, no. 3, 30 May 2000 (2000-05-30), pages 21 - 2 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322276A (en) * | 2014-09-26 | 2015-02-04 | 浙江省农业科学院 | Method for improving preparing efficiency and yield and quality of shiitake sticks |
| CN104488558A (en) * | 2014-12-31 | 2015-04-08 | 天津市金三农农业科技开发有限公司 | Method for increasing spawn running rate of mushroom |
| CN104956919A (en) * | 2015-07-08 | 2015-10-07 | 翔天农业开发集团股份有限公司 | Inoculation method for mushroom wood flour liquid strains |
| CN105145110A (en) * | 2015-07-08 | 2015-12-16 | 翔天农业开发集团股份有限公司 | Mushroom wood powder solid and liquid mixing bacterial strain inoculation method |
| CN104956919B (en) * | 2015-07-08 | 2018-04-17 | 翔天农业开发集团股份有限公司 | A kind of mushroom wood powder liquid spawn inoculation method |
| CN105145110B (en) * | 2015-07-08 | 2018-04-17 | 翔天农业开发集团股份有限公司 | A kind of mushroom wood powder solid and liquid mixed bacteria inoculation method |
| CN105009944A (en) * | 2015-08-03 | 2015-11-04 | 廊坊市森淼春食用菌有限公司 | Fast inoculation method for mushroom stick |
| CN109418025A (en) * | 2017-06-29 | 2019-03-05 | 祁海杰 | A kind of cultivating champignon inoculation method |
| CN107853081A (en) * | 2017-10-27 | 2018-03-30 | 黄兴根 | A kind of inoculation method of mushroom culture medium |
| CN108391557A (en) * | 2018-05-15 | 2018-08-14 | 务川自治县惠农现代农业开发有限公司 | A kind of breeding method of mushroom |
| CN109275492A (en) * | 2018-06-14 | 2019-01-29 | 陕西蓝米信息技术有限公司 | A kind of mushroom implantation methods |
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